##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3317993 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82152343299097 31.0 31.0 34.0 30.0 34.0 2 32.05290788738855 33.0 31.0 34.0 30.0 34.0 3 32.117734124213044 33.0 31.0 34.0 30.0 34.0 4 35.789985994545496 37.0 35.0 37.0 35.0 37.0 5 35.657759977191034 37.0 35.0 37.0 33.0 37.0 6 35.700674172609766 37.0 35.0 37.0 33.0 37.0 7 35.63414901719202 37.0 35.0 37.0 33.0 37.0 8 35.613926551382114 37.0 35.0 37.0 33.0 37.0 9 37.18999407171745 39.0 37.0 39.0 33.0 39.0 10-11 37.124226151170305 39.0 37.0 39.0 33.0 39.0 12-13 37.02007478617345 39.0 37.0 39.0 33.0 39.0 14-15 38.07847997268228 40.0 37.0 41.0 33.0 41.0 16-17 38.001544005668485 40.0 37.0 41.0 33.0 41.0 18-19 38.012809400140384 40.0 37.0 41.0 33.0 41.0 20-21 38.00689437861985 40.0 37.0 41.0 33.0 41.0 22-23 38.03466628772273 40.0 37.0 41.0 33.0 41.0 24-25 37.94959950186754 40.0 37.0 41.0 33.0 41.0 26-27 37.775177494346735 40.0 37.0 41.0 32.0 41.0 28-29 37.64701040659217 40.0 37.0 41.0 32.0 41.0 30-31 37.387778696338415 39.0 36.0 41.0 31.5 41.0 32-33 37.146831985480375 39.0 36.0 41.0 31.0 41.0 34-35 36.970076036929555 39.0 36.0 41.0 31.0 41.0 36-37 36.95450563036149 39.0 36.0 41.0 31.0 41.0 38-39 36.79516728938247 39.0 35.0 41.0 30.0 41.0 40-41 36.60730673633127 39.0 35.0 41.0 30.0 41.0 42-43 36.36955126186221 39.0 35.0 40.0 30.0 41.0 44-45 36.084594361712035 38.5 35.0 40.0 29.5 41.0 46-47 35.70393367315724 38.0 35.0 40.0 28.5 41.0 48-49 35.27994423134709 38.0 34.0 40.0 27.0 41.0 50-51 34.88760826198247 38.0 34.0 40.0 26.0 41.0 52-53 34.36659058653831 38.0 33.0 40.0 24.0 41.0 54-55 34.0359033307183 37.0 33.0 40.0 23.5 41.0 56-57 33.66587331558566 37.0 33.0 40.0 23.0 41.0 58-59 33.195728261030084 36.5 32.0 39.0 22.0 41.0 60-61 32.78174547083131 36.0 31.0 39.0 20.5 40.0 62-63 32.30328590204982 35.5 31.0 39.0 18.5 40.0 64-65 31.807451221265385 35.0 30.5 38.5 17.5 40.0 66-67 31.3125304965984 35.0 30.0 38.0 13.5 40.0 68-69 30.749889164925904 34.0 29.0 37.5 9.5 39.5 70-71 30.171221277440914 34.0 29.0 37.0 7.5 39.0 72-73 29.594957704853506 34.0 28.0 36.0 6.5 39.0 74-75 28.99984282667263 33.5 27.5 36.0 2.0 38.5 76-77 27.716506936572802 31.5 26.0 34.5 2.0 36.5 78-79 28.33916919053175 33.0 27.0 35.0 2.0 37.0 80-81 28.04872734812882 33.0 26.0 35.0 2.0 36.5 82-83 27.56626023623317 33.0 26.0 35.0 2.0 36.0 84-85 26.990938196674918 32.5 25.0 35.0 2.0 36.0 86-87 26.36504567670878 32.0 24.5 35.0 2.0 35.0 88-89 25.609861443348432 31.5 21.5 34.0 2.0 35.0 90-91 25.074725745352687 31.0 19.5 34.0 2.0 35.0 92-93 24.31899223416083 31.0 16.5 34.0 2.0 35.0 94-95 23.539474314743885 31.0 4.5 34.0 2.0 35.0 96-97 22.57208197847313 30.0 2.0 34.0 2.0 35.0 98-99 21.40357378692481 29.5 2.0 34.0 2.0 35.0 100-101 18.23684965580096 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2548716112880669 1101 2 0.334617919008771 1101 3 0.31116317633684787 1101 4 0.25023893658925545 1101 5 0.13172284496781828 1101 6 0.2878684801962237 1101 7 0.3041372872106365 1101 8 0.3058806964879679 1101 9 0.394262050247967 1101 10-11 0.4255863787876919 1101 12-13 0.37705271638396454 1101 14-15 0.5424782638060179 1101 16-17 0.544689691597469 1101 18-19 0.6103285283485178 1101 20-21 0.568457098391633 1101 22-23 0.5940862394291457 1101 24-25 0.533442639202363 1101 26-27 0.5655363592992515 1101 28-29 0.5579476194336692 1101 30-31 0.6111009096334357 1101 32-33 0.49476127208648535 1101 34-35 0.49727085331205245 1101 36-37 0.6569057265084979 1101 38-39 0.818429113694016 1101 40-41 0.8738101141790153 1101 42-43 0.7522811614010934 1101 44-45 0.8056617814631792 1101 46-47 0.9117026919850488 1101 48-49 1.0461250840414849 1101 50-51 1.0173018585715567 1101 52-53 0.9693850401823454 1101 54-55 1.0595116508644296 1101 56-57 1.293717182572017 1101 58-59 1.3027278380853673 1101 60-61 1.3745556800193057 1101 62-63 1.424629894378043 1101 64-65 1.4423027088560474 1101 66-67 1.5264738169136542 1101 68-69 1.5633999489663317 1101 70-71 1.614892551993865 1101 72-73 1.665708567248526 1101 74-75 1.8070171766718701 1101 76-77 1.5205338785347813 1101 78-79 1.8564320877992806 1101 80-81 2.223686763314781 1101 82-83 2.2825130791890906 1101 84-85 2.595102110112478 1101 86-87 2.6590071494829814 1101 88-89 2.764009814107432 1101 90-91 3.0673391441297397 1101 92-93 3.5376788438372273 1101 94-95 3.4961410516453135 1101 96-97 3.7862581840255913 1101 98-99 4.046745744358105 1101 100-101 4.438425116879252 1102 1 0.2975393016539698 1102 2 0.2667627136855373 1102 3 0.2958775242225542 1102 4 0.21354800603643298 1102 5 0.09804680234036312 1102 6 0.12827870935325336 1102 7 0.2424355618260492 1102 8 0.2588075650158572 1102 9 0.37514178432463297 1102 10-11 0.35358676714613324 1102 12-13 0.3773322748476673 1102 14-15 0.5180396802242058 1102 16-17 0.49272886470058097 1102 18-19 0.556481267340132 1102 20-21 0.531425608994418 1102 22-23 0.4988840205056917 1102 24-25 0.45961674216674453 1102 26-27 0.45967749097234645 1102 28-29 0.3758362525396919 1102 30-31 0.4492261262752777 1102 32-33 0.3270178901487242 1102 34-35 0.40471336817144987 1102 36-37 0.4677297064501218 1102 38-39 0.5871179057204046 1102 40-41 0.6199768843227602 1102 42-43 0.6228005610125606 1102 44-45 0.6265506113034149 1102 46-47 0.734798287778851 1102 48-49 0.8502635074254314 1102 50-51 1.0257604715698179 1102 52-53 0.9874768162664438 1102 54-55 0.8251423330552115 1102 56-57 0.9244292880283709 1102 58-59 0.9646223161563157 1102 60-61 1.1682263103267587 1102 62-63 1.2573078728360727 1102 64-65 1.1276214395711932 1102 66-67 1.1531113524386534 1102 68-69 1.4867263029192834 1102 70-71 1.4298706259233462 1102 72-73 1.3842877289223132 1102 74-75 1.5552769092666452 1102 76-77 1.6991557681541138 1102 78-79 1.9768849512188957 1102 80-81 2.116140643620323 1102 82-83 2.063949034538094 1102 84-85 2.2168700480568866 1102 86-87 2.2540290287120186 1102 88-89 2.453502972270666 1102 90-91 2.678123393980485 1102 92-93 2.946440585079788 1102 94-95 3.0965915389244465 1102 96-97 3.5737300897839397 1102 98-99 3.9441534304486545 1102 100-101 4.514197170912176 1103 1 0.2782634587560153 1103 2 0.27152453016756084 1103 3 0.2614481690724091 1103 4 0.24867684338436646 1103 5 0.16950644149433458 1103 6 0.15567229424485163 1103 7 0.2837497286477131 1103 8 0.24861266546036376 1103 9 0.33889369397113 1103 10-11 0.34767386597267347 1103 12-13 0.44035351233730324 1103 14-15 0.49347656883938384 1103 16-17 0.4785956471089605 1103 18-19 0.46158607151058106 1103 20-21 0.4892552462191304 1103 22-23 0.4705920690430574 1103 24-25 0.41254012345167723 1103 26-27 0.43585246725392324 1103 28-29 0.36110083038227714 1103 30-31 0.45021672833927084 1103 32-33 0.5159544795969921 1103 34-35 0.5728026480541715 1103 36-37 0.6214228644813531 1103 38-39 0.7373477253992462 1103 40-41 0.6599783124195184 1103 42-43 0.6950853283947467 1103 44-45 0.8148297363792238 1103 46-47 0.8500815510756894 1103 48-49 0.9397430384800245 1103 50-51 0.8676116924091914 1103 52-53 1.0757007528385785 1103 54-55 1.1182317911953774 1103 56-57 1.1620946880146406 1103 58-59 1.2965523899386042 1103 60-61 1.1818278114377634 1103 62-63 1.295878555782096 1103 64-65 1.3603343385444475 1103 66-67 1.4047673131331955 1103 68-69 1.452332039019634 1103 70-71 1.5473168650071152 1103 72-73 1.4482536289869152 1103 74-75 1.6605922711261911 1103 76-77 1.5039684032859881 1103 78-79 1.7914213769555225 1103 80-81 2.032567545540637 1103 82-83 2.162271764439769 1103 84-85 2.2277447196302838 1103 86-87 2.4803264933207885 1103 88-89 2.5944540847091453 1103 90-91 2.9384418004771256 1103 92-93 3.0002271194515906 1103 94-95 2.9777816813490823 1103 96-97 3.498268010845841 1103 98-99 3.805191874772543 1103 100-101 4.106522352821347 1104 1 0.33390474561678474 1104 2 0.3211085210366349 1104 3 0.2653143312935953 1104 4 0.21009098150231154 1104 5 0.20025139548901905 1104 6 0.13892973843672962 1104 7 0.26000983809562683 1104 8 0.265024318467745 1104 9 0.27883817519845877 1104 10-11 0.3479180355998608 1104 12-13 0.39763976205690454 1104 14-15 0.49901899991967014 1104 16-17 0.5168245576133899 1104 18-19 0.6083600220558765 1104 20-21 0.5086439748567955 1104 22-23 0.5145276216197274 1104 24-25 0.5562044654975367 1104 26-27 0.5520648057110051 1104 28-29 0.49100045557855765 1104 30-31 0.5038137267035552 1104 32-33 0.5559414305003756 1104 34-35 0.6488569069535473 1104 36-37 0.7161390680987907 1104 38-39 0.7520541030736467 1104 40-41 0.8824223292093052 1104 42-43 0.8508140258828902 1104 44-45 0.7449704588064421 1104 46-47 0.7887534686774558 1104 48-49 0.8463831737646856 1104 50-51 0.9225402692151334 1104 52-53 1.0702681456117062 1104 54-55 1.0747823833043384 1104 56-57 1.1387222923117548 1104 58-59 1.2975637998831857 1104 60-61 1.1979592752656885 1104 62-63 1.2996396172434785 1104 64-65 1.407114910962406 1104 66-67 1.619445800701289 1104 68-69 1.7109881099267277 1104 70-71 1.7427557114883463 1104 72-73 1.9117117216452328 1104 74-75 1.9370706733732774 1104 76-77 1.8822253699206222 1104 78-79 2.1007521393992725 1104 80-81 2.293411076700984 1104 82-83 2.449825344120125 1104 84-85 2.58847934293113 1104 86-87 2.6640254942863066 1104 88-89 2.873413590049534 1104 90-91 2.906634388960466 1104 92-93 3.3192658907068235 1104 94-95 3.6418990701672236 1104 96-97 3.909823837001266 1104 98-99 4.2214259967098435 1104 100-101 4.474174447646945 1105 1 0.28223852769070845 1105 2 0.3058258514516794 1105 3 0.2327315756413597 1105 4 0.1867430130761818 1105 5 0.19633402828348068 1105 6 0.18054639525961846 1105 7 0.23326971165862176 1105 8 0.20494183041106595 1105 9 0.32343345352055763 1105 10-11 0.27535588012634804 1105 12-13 0.3791146186359313 1105 14-15 0.4281467293163459 1105 16-17 0.46054691739888654 1105 18-19 0.4419962441467291 1105 20-21 0.3806507704024682 1105 22-23 0.3108870921539548 1105 24-25 0.3570006747937171 1105 26-27 0.5065828748056447 1105 28-29 0.5439297869122939 1105 30-31 0.693899884005539 1105 32-33 0.6351778335491076 1105 34-35 0.5908874211353989 1105 36-37 0.7836675305958849 1105 38-39 0.8138492472459404 1105 40-41 0.7616474313033592 1105 42-43 0.8069222498139865 1105 44-45 0.7683273396573966 1105 46-47 0.8372808197060593 1105 48-49 0.9354850963440597 1105 50-51 0.9338672481678216 1105 52-53 1.014172630798413 1105 54-55 1.0251224858916856 1105 56-57 1.0698015308092224 1105 58-59 1.360915320801844 1105 60-61 1.2482307878316234 1105 62-63 1.2873477346176116 1105 64-65 1.5235929506139598 1105 66-67 1.5466019680413403 1105 68-69 1.6334089722733403 1105 70-71 1.5828725141630748 1105 72-73 1.619478614678581 1105 74-75 1.7228137525857505 1105 76-77 1.6918938090012112 1105 78-79 1.9998568061142485 1105 80-81 2.190138309533001 1105 82-83 2.368940187041755 1105 84-85 2.4631842414047966 1105 86-87 2.664773736912096 1105 88-89 2.9501849946414467 1105 90-91 3.0742326268314812 1105 92-93 3.1565026878629787 1105 94-95 3.3428976449813668 1105 96-97 3.6295232636917163 1105 98-99 3.8710666789345716 1105 100-101 4.4755617556944784 1106 1 0.1523817539201282 1106 2 0.18745457108334307 1106 3 0.1538254356929869 1106 4 0.13196343931236498 1106 5 0.03188574448422088 1106 6 0.06983246420547573 1106 7 0.05109865319194995 1106 8 0.048747609245303636 1106 9 0.1093244376757383 1106 10-11 0.20780237024852255 1106 12-13 0.03855110302176712 1106 14-15 -0.044622566087070936 1106 16-17 0.07923370949266939 1106 18-19 0.26070088988887363 1106 20-21 0.3773745507635127 1106 22-23 0.2544439633635349 1106 24-25 0.13210395226903415 1106 26-27 0.06292342691402553 1106 28-29 0.28851751840510076 1106 30-31 0.26587820707663923 1106 32-33 0.32580496881318055 1106 34-35 0.2843522607227129 1106 36-37 0.37081221403318665 1106 38-39 0.3658218012629533 1106 40-41 0.3609633244195223 1106 42-43 0.5122815631090774 1106 44-45 0.0078023309759629456 1106 46-47 -0.13879798854390657 1106 48-49 0.3363062226837812 1106 50-51 0.20597603816708698 1106 52-53 0.4322244290162871 1106 54-55 0.6087646619923319 1106 56-57 0.47415872297771244 1106 58-59 0.7873931302378949 1106 60-61 0.7368409294997562 1106 62-63 0.7567999565656081 1106 64-65 0.36902147487241876 1106 66-67 0.6807359683824963 1106 68-69 0.5104904056905042 1106 70-71 0.5900448349419349 1106 72-73 0.9554517353433312 1106 74-75 1.40061924473585 1106 76-77 1.3473626096401574 1106 78-79 1.558067039323216 1106 80-81 1.5036037185977555 1106 82-83 1.8498498264466932 1106 84-85 1.8765304669764014 1106 86-87 1.9946278932087154 1106 88-89 2.309438733362544 1106 90-91 2.3748876231603866 1106 92-93 1.9685485594477576 1106 94-95 2.377419915749673 1106 96-97 3.022221952735187 1106 98-99 3.3827183288760807 1106 100-101 3.8432378358645245 1107 1 0.1864370059144349 1107 2 0.20175079342447333 1107 3 0.17083769247186353 1107 4 0.1343260680738183 1107 5 0.11970096779852923 1107 6 0.10009929023999575 1107 7 0.21004080544028625 1107 8 0.15936410399114465 1107 9 0.22441518816070527 1107 10-11 0.2222372609297807 1107 12-13 0.2150222804273625 1107 14-15 0.35387596490058826 1107 16-17 0.3463707548260757 1107 18-19 0.26609902560269916 1107 20-21 0.3093462030175118 1107 22-23 0.24277561831491568 1107 24-25 0.24196532623743394 1107 26-27 0.3524617986282408 1107 28-29 0.28834979533493055 1107 30-31 0.2585799869961676 1107 32-33 0.3604622538096791 1107 34-35 0.3627807771502063 1107 36-37 0.40093592501555975 1107 38-39 0.45007684877758436 1107 40-41 0.4137319811610638 1107 42-43 0.46307524076528495 1107 44-45 0.4961505639605335 1107 46-47 0.686954664955941 1107 48-49 0.6680259320052357 1107 50-51 0.6333341619343642 1107 52-53 0.7190026877201205 1107 54-55 0.6061613495458857 1107 56-57 0.6696501282604501 1107 58-59 0.7833572857671314 1107 60-61 0.7822000688442543 1107 62-63 0.9464995841901711 1107 64-65 1.032802038354589 1107 66-67 1.2307969228748483 1107 68-69 1.2137695579244259 1107 70-71 1.151207372973193 1107 72-73 1.329929257473065 1107 74-75 1.3431661711469118 1107 76-77 1.4068872897985258 1107 78-79 1.5664322544305342 1107 80-81 1.7896379948901924 1107 82-83 1.9938249935891008 1107 84-85 2.155212774096384 1107 86-87 2.3244083180225488 1107 88-89 2.5843371044067283 1107 90-91 2.6825032607701047 1107 92-93 2.9108960313969945 1107 94-95 2.9333909390745383 1107 96-97 3.400996207498629 1107 98-99 3.79775012373052 1107 100-101 3.9939050887488676 1108 1 0.18645205720230962 1108 2 0.2028300928024791 1108 3 0.1617882073357464 1108 4 0.13458405528380268 1108 5 0.0055320677783825545 1108 6 0.08406560073895264 1108 7 0.10218986656128948 1108 8 0.13699422908687353 1108 9 0.16055143469657196 1108 10-11 0.1810369638417697 1108 12-13 0.2950421269741952 1108 14-15 0.32998595060023916 1108 16-17 0.36538228061418465 1108 18-19 0.4107379561899833 1108 20-21 0.39574079880785007 1108 22-23 0.29993276197750873 1108 24-25 0.2818131227329559 1108 26-27 0.4025309653978937 1108 28-29 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0.6159255212542583 2316 72-73 0.6887148252046167 2316 74-75 0.8103901971853631 2316 76-77 1.0029820323876173 2316 78-79 1.0876576626703418 2316 80-81 1.1629723940719856 2316 82-83 1.3750225763808643 2316 84-85 1.536458830315354 2316 86-87 1.6587536404502075 2316 88-89 2.1277194044056493 2316 90-91 2.0632277315735124 2316 92-93 2.0900643067578066 2316 94-95 2.7457097660969687 2316 96-97 2.9065578954624236 2316 98-99 3.2084946699030183 2316 100-101 3.5065189516954938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 55.0 5 121.0 6 225.0 7 651.0 8 1478.0 9 2786.0 10 4455.0 11 6995.0 12 9935.0 13 13872.0 14 17979.0 15 23894.0 16 30288.0 17 38192.0 18 36390.0 19 32303.0 20 31964.0 21 33214.0 22 36188.0 23 40431.0 24 46607.0 25 54668.0 26 64042.0 27 76713.0 28 90897.0 29 108296.0 30 126717.0 31 151048.0 32 180605.0 33 219872.0 34 275619.0 35 350731.0 36 442563.0 37 486498.0 38 265180.0 39 16494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44475045290961 19.028653182000795 15.124965679533187 23.40163068555641 2 15.988833850150701 20.463796852781226 38.8279100677471 24.71945922932097 3 19.23343303230753 23.6438468131892 30.151796811802818 26.970923342700452 4 13.37251163579911 16.878757730953623 35.90917762635424 33.83955300689303 5 13.702619860740706 37.45354000989538 34.09949075023833 14.744349379125588 6 33.04720654926035 36.051613128779955 16.632856066905504 14.268324255054186 7 29.185353917262635 32.85187762602272 19.833917672520705 18.12885078419394 8 27.342342193006434 34.435997905963035 19.28117389036083 18.9404860106697 9 26.67326302376165 16.384302197141466 19.583254093664454 37.35918068543243 10-11 25.222566774553172 26.40236130697081 26.99543067149328 21.37964124698274 12-13 26.350748780964878 23.594670030949434 27.29253798907954 22.762043199006147 14-15 22.481738207404295 25.21364571896324 25.66604269508706 26.638573378545406 16-17 22.199715309827354 28.426144961728372 26.810002311638392 22.56413741680588 18-19 22.44076464296338 26.494389831443282 29.183711358040842 21.881134167552492 20-21 22.626886192948568 25.732830057206268 29.592331870501233 22.04795187934393 22-23 22.477805106882386 25.737712526819678 29.58591232712064 22.198570039177298 24-25 22.40429228438554 25.99974653050442 29.542939646381424 22.05302153872862 26-27 21.731651289730532 26.420976293023546 29.180309684832338 22.66706273241358 28-29 22.065869337277082 27.00704914085111 28.91448233917311 22.012599182698697 30-31 22.10489895548303 26.31782224977569 28.852185643550182 22.725093151191096 32-33 21.58766779787702 26.359200477398897 28.953173316294855 23.099958408429224 34-35 22.711345537159406 26.5220632995545 28.62359331685813 22.14299784642796 36-37 22.872717732160762 25.819621177860203 28.695756745364786 22.61190434461425 38-39 22.13288441714241 26.2427131497881 28.737830131238656 22.886572301830828 40-41 22.70468098445198 26.046353214569358 28.86419704712632 22.384768753852345 42-43 22.389995643242603 26.246728099306942 28.49088765578874 22.872388601661715 44-45 22.305197804461574 26.45113543599202 28.870338726737803 22.3733280328086 46-47 22.921800293671595 26.840043616741692 27.88462118052123 22.353534909065484 48-49 22.47658448947903 26.900132097927877 27.70619166465993 22.917091747933164 50-51 22.802941416693766 27.0471938910058 27.758482311445505 22.39138238085493 52-53 23.244473390992688 26.596198364493233 27.78984163016619 22.369486614347892 54-55 22.366593299021424 27.0272872787857 28.08200620073641 22.524113221456467 56-57 22.559330293945766 27.045762302693227 27.72011574466854 22.67479165869247 58-59 22.4423619941332 26.616180323466626 27.720176022071175 23.221281660328998 60-61 21.8358507688232 26.981401106030063 28.17521013456026 23.007537990586478 62-63 22.219079425423743 26.93426417716975 28.247452601617905 22.5992037957886 64-65 21.851688656365457 27.068893755954278 27.916439245049645 23.16297834263062 66-67 21.69245066616556 27.08239934430014 28.02194647559066 23.20320351394364 68-69 22.671452301403967 26.45784618407766 28.092889495749755 22.777812018768618 70-71 22.23372683426397 26.80594865631121 27.49100133725418 23.469323172170647 72-73 22.220328506014635 26.936149013062423 27.572166410336855 23.271356070586087 74-75 22.205396455025674 27.32251092753963 27.40658283486433 23.065509782570366 76-77 22.262328461814114 26.822856467750235 27.785818113540323 23.128996956895328 78-79 22.313925918469387 27.104668394417953 27.694241669587612 22.887164017525052 80-81 22.60835626388265 27.068646982058414 27.358747313885818 22.964249440173116 82-83 22.356960300907787 27.167461331661624 27.533288526685073 22.94228984074552 84-85 22.186799338969315 27.343182284666963 27.50147761396013 22.968540762403595 86-87 22.55511924104784 27.365268179712547 26.9629104538565 23.116702125383107 88-89 22.212435508781596 27.484994306041564 26.760203201305128 23.542366983871712 90-91 22.046310972311005 27.405038817514733 27.112298788322104 23.436351421852155 92-93 22.288700005786648 26.90692601290422 27.359324216150533 23.4450497651586 94-95 21.860353532994193 26.648941091798566 27.628102289546725 23.86260308566052 96-97 22.202473000997895 26.118997237185248 27.669784113468594 24.008745648348263 98-99 21.82815033063662 26.561177193562497 27.190126682003246 24.420545793797636 100-101 21.76295197453623 26.134376180127063 27.81309891683738 24.289572928499332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 655.0 1 465.5 2 384.5 3 519.0 4 458.5 5 465.5 6 848.5 7 1883.5 8 2961.5 9 3316.0 10 2898.0 11 2329.5 12 2280.0 13 2455.0 14 2694.0 15 2949.0 16 3226.0 17 3564.0 18 3978.5 19 4475.0 20 5195.5 21 6329.5 22 7657.0 23 9275.5 24 11382.5 25 14158.0 26 18032.0 27 22979.0 28 27629.5 29 32332.0 30 37928.5 31 46642.0 32 61650.5 33 65980.0 34 61591.0 35 64682.0 36 70786.0 37 77365.0 38 83255.5 39 87056.5 40 91928.5 41 98868.5 42 102766.0 43 105499.5 44 109053.0 45 116434.0 46 121197.5 47 123478.5 48 124061.0 49 121314.0 50 120697.0 51 118139.0 52 114209.5 53 116442.0 54 121712.0 55 115529.0 56 104776.0 57 95629.0 58 88354.0 59 77437.0 60 60443.5 61 50177.5 62 44084.0 63 32827.5 64 21632.5 65 16054.5 66 12169.5 67 9552.0 68 7415.0 69 5762.0 70 4558.0 71 2985.0 72 2116.0 73 1781.5 74 1377.5 75 1044.5 76 780.0 77 524.5 78 330.0 79 203.0 80 123.0 81 64.0 82 30.0 83 18.0 84 12.0 85 8.0 86 5.5 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.0277402634664994E-5 2 0.04644373873000937 3 1.5069350658666249E-4 4 0.0 5 0.09984951746432255 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011904787020346336 26-27 0.010910209876874364 28-29 0.0 30-31 0.0 32-33 3.918031171253225E-4 34-35 0.014511784684295597 36-37 0.003119355586343913 38-39 0.011241735591365022 40-41 0.00626884987400516 42-43 0.0047769841587972 44-45 0.0024864428586799307 46-47 3.0138701317332497E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.465950651493237E-4 68-69 2.2604025987999374E-4 70-71 0.0 72-73 6.0277402634664994E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.041610395199749E-5 82-83 9.945771434719723E-4 84-85 0.004339972989695879 86-87 0.001506935065866625 88-89 7.534675329333124E-5 90-91 9.644384421546399E-4 92-93 2.7124831185599245E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.233288316159799E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3317993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.79504555547208 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66384768986788 34.72971910573119 2 14.568252684377414 13.925806014268938 3 5.194841827439286 7.448631053877975 4 2.4754206769473384 4.7325137609462224 5 1.4139404593080824 3.378967433267746 6 0.816392104283658 2.341169868921909 7 0.5694986207647609 1.9053448766261183 8 0.4115106673185199 1.5734536872841098 9 0.2817043346471958 1.2117664356873008 >10 1.4420935461938096 12.208056766333904 >50 0.0988100576567379 3.262725974237269 >100 0.05281161989548965 4.914735471020301 >500 0.005943702688135559 1.9032422591789178 >1k 0.004362930695016472 4.523271596515622 >5k 5.05847037103359E-4 1.6019668394095095 >10k+ 6.323087963791988E-5 0.338628856693019 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11205 0.3377041482607106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9137 0.275377313936467 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6947 0.20937355805150884 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6715 0.20238137934588768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6679 0.20129638609846373 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6288 0.18951215388338674 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6096 0.18372552323045888 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5643 0.17007269153370727 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5503 0.1658532733492807 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4788 0.144304101907388 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4526 0.13640776216224687 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4296 0.1294758608592604 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4225 0.1273360130657298 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4035 0.12160965981543662 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3804 0.1146476198111328 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3596 0.10837876993712765 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3586 0.10807738292395433 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3556 0.10717322188443436 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3464 0.10440046136323976 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3454 0.10409907435006643 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0138701317332497E-5 2 0.0 0.0 0.0 0.0 3.0138701317332497E-5 3 0.0 0.0 0.0 0.0 3.0138701317332497E-5 4 0.0 0.0 0.0 0.0 3.0138701317332497E-5 5 0.0 0.0 0.0 0.0 3.0138701317332497E-5 6 0.0 0.0 0.0 0.0 6.0277402634664994E-5 7 0.0 0.0 0.0 0.0 6.0277402634664994E-5 8 0.0 0.0 0.0 0.0 6.0277402634664994E-5 9 0.0 0.0 0.0 0.0 6.0277402634664994E-5 10-11 0.0 0.0 0.0 0.0 6.0277402634664994E-5 12-13 1.5069350658666249E-5 0.0 0.0 0.0 3.1645636383199123E-4 14-15 3.0138701317332497E-5 0.0 0.0 0.0 4.068724677839887E-4 16-17 3.0138701317332497E-5 0.0 0.0 0.0 4.8221922107731996E-4 18-19 7.534675329333124E-5 0.0 0.0 1.5069350658666249E-5 5.877046756879837E-4 20-21 1.2055480526932999E-4 0.0 0.0 4.5208051975998746E-5 6.931901302986473E-4 22-23 1.2055480526932999E-4 0.0 0.0 3.465950651493237E-4 7.534675329333123E-4 24-25 1.5069350658666249E-4 0.0 0.0 6.027740263466498E-4 7.534675329333123E-4 26-27 1.5069350658666249E-4 0.0 0.0 0.0012959641566452974 7.534675329333123E-4 28-29 1.5069350658666249E-4 0.0 0.0 0.003782407015325228 7.534675329333123E-4 30-31 1.5069350658666249E-4 0.0 0.0 0.012673323903938315 7.836062342506449E-4 32-33 1.5069350658666249E-4 0.0 0.0 0.03135931872068446 8.740223382026423E-4 34-35 1.9590155856266125E-4 0.0 0.0 0.058273178997062386 9.041610395199749E-4 36-37 2.1097090922132748E-4 0.0 0.0 0.10349630032371979 9.041610395199749E-4 38-39 2.1097090922132748E-4 0.0 0.0 0.17720049439525642 9.493690914959736E-4 40-41 2.1097090922132748E-4 0.0 0.0 0.2564954175611582 9.945771434719723E-4 42-43 2.1097090922132748E-4 3.0138701317332497E-5 0.0 0.33802060462454264 9.945771434719723E-4 44-45 2.1097090922132748E-4 3.0138701317332497E-5 0.0 0.44029628754491046 0.0010548545461066374 46-47 3.0138701317332497E-4 3.0138701317332497E-5 0.0 0.5648294013881283 0.0011151319487413024 48-49 3.0138701317332497E-4 3.0138701317332497E-5 0.0 0.6962492084823566 0.0012055480526932999 50-51 3.0138701317332497E-4 3.0138701317332497E-5 0.0 0.8444412028596806 0.0012959641566452974 52-53 3.0138701317332497E-4 3.0138701317332497E-5 0.0 0.9845258865826421 0.0014165189619146272 54-55 3.0138701317332497E-4 3.0138701317332497E-5 0.0 1.1367866056378055 0.0014466576632319599 56-57 3.3152571449065744E-4 3.0138701317332497E-5 0.0 1.313821337175817 0.0014767963645492923 58-59 3.3152571449065744E-4 4.5208051975998746E-5 0.0 1.5034540458644727 0.0015370737671839573 60-61 3.3152571449065744E-4 6.0277402634664994E-5 0.0 1.7117275413178992 0.0016274898711359548 62-63 3.3152571449065744E-4 6.0277402634664994E-5 0.0 1.917891327679112 0.0016877672737706198 64-65 3.3152571449065744E-4 6.0277402634664994E-5 0.0 2.1326446439157647 0.0017179059750879523 66-67 3.9180311712532244E-4 6.0277402634664994E-5 0.0 2.3872563926445896 0.0017480446764052847 68-69 3.9180311712532244E-4 6.0277402634664994E-5 0.0 2.670741017235419 0.0017480446764052847 70-71 4.219418184426549E-4 6.0277402634664994E-5 0.0 2.9632220441694725 0.0017480446764052847 72-73 4.5208051975998743E-4 6.0277402634664994E-5 0.0 3.2788043856632605 0.0018233914296986158 74-75 4.5208051975998743E-4 6.0277402634664994E-5 0.0 3.5922318100128603 0.001959015585626612 76-77 4.5208051975998743E-4 6.0277402634664994E-5 0.0 3.9309154660663843 0.0020042236376026108 78-79 4.5208051975998743E-4 6.0277402634664994E-5 0.0 4.313466001887285 0.00204943168957861 80-81 5.424966237119849E-4 6.0277402634664994E-5 0.0 4.718530147592234 0.0020946397415546087 82-83 5.424966237119849E-4 6.0277402634664994E-5 0.0 5.1421597333086595 0.0022151945468239388 84-85 5.575659743706511E-4 6.0277402634664994E-5 0.0 5.572706150977413 0.002260402598799937 86-87 5.726353250293174E-4 6.0277402634664994E-5 0.0 6.016046447355374 0.0023658880534106006 88-89 5.726353250293174E-4 6.0277402634664994E-5 0.0 6.488229480894022 0.0024713735080212646 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5835 0.0 58.36651 1 GTATCAA 10910 0.0 43.162045 1 ATCAACG 14290 0.0 32.538807 3 TCAACGC 14395 0.0 32.36752 4 TATCAAC 14500 0.0 32.114876 2 CAACGCA 14800 0.0 31.481787 5 AACGCAG 15365 0.0 30.35489 6 TAAGGTG 2440 0.0 30.20208 5 GTGTAAG 1255 0.0 27.667551 1 AGGTAAG 2345 0.0 27.58593 2 TGGTATC 2465 0.0 27.014858 2 ACGCAGA 17550 0.0 26.494476 7 CGCAGAG 17595 0.0 26.372725 8 GTAAGGT 2785 0.0 25.60714 4 GGTAAGG 2855 0.0 24.812763 3 GTGGTAT 2850 0.0 24.533754 1 AAGGTAA 2650 0.0 24.231455 1 GTACATG 12475 0.0 24.059155 1 TACATGG 12135 0.0 23.949308 2 TAAGGTA 1365 0.0 23.336576 4 >>END_MODULE