##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3305834 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10547414056483 31.0 31.0 34.0 28.0 34.0 2 31.28393379703881 31.0 31.0 34.0 28.0 34.0 3 31.335962725291107 31.0 31.0 34.0 28.0 34.0 4 35.00803246623999 37.0 35.0 37.0 32.0 37.0 5 35.00700640141036 37.0 35.0 37.0 32.0 37.0 6 34.98152962308452 37.0 35.0 37.0 32.0 37.0 7 34.903784037553 37.0 35.0 37.0 32.0 37.0 8 34.84976771368435 37.0 35.0 37.0 32.0 37.0 9 36.338867589842685 39.0 35.0 39.0 32.0 39.0 10-11 36.18333543063566 39.0 35.0 39.0 32.0 39.0 12-13 36.009361480340516 38.0 35.0 39.0 30.0 39.0 14-15 37.15946248359718 39.0 36.0 41.0 31.0 41.0 16-17 37.096003308091085 39.0 36.0 41.0 31.0 41.0 18-19 37.10053166613932 39.5 36.0 41.0 31.0 41.0 20-21 37.031960316216725 39.0 36.0 41.0 31.0 41.0 22-23 36.89550050002511 39.0 36.0 41.0 30.5 41.0 24-25 36.75257151448016 39.0 36.0 41.0 30.0 41.0 26-27 36.5350799525929 39.0 36.0 41.0 30.0 41.0 28-29 36.30389925809946 39.0 35.5 40.5 29.5 41.0 30-31 36.01293092756623 38.5 35.0 40.0 28.5 41.0 32-33 35.703219822894916 38.0 35.0 40.0 27.0 41.0 34-35 35.39786873751072 38.0 34.0 40.0 26.5 41.0 36-37 35.15027539192833 38.0 34.0 40.0 25.5 41.0 38-39 34.90156432537145 38.0 34.0 40.0 25.0 41.0 40-41 34.61413988119186 38.0 33.0 40.0 24.0 41.0 42-43 34.19010013811946 38.0 33.0 40.0 23.5 41.0 44-45 33.72766675519702 38.0 33.0 40.0 22.0 41.0 46-47 33.23391495156744 37.5 32.0 40.0 18.5 41.0 48-49 32.727516112424276 37.0 31.0 40.0 15.5 41.0 50-51 31.972042304604525 36.0 30.5 39.0 13.0 40.5 52-53 31.953851736052084 36.0 31.0 39.0 10.5 40.0 54-55 31.939518287972113 36.0 31.0 39.0 8.5 40.0 56-57 31.520004029240425 36.0 30.0 39.0 6.5 40.0 58-59 31.075133385402896 35.5 29.5 39.0 2.0 40.0 60-61 30.531106220094536 35.0 28.5 39.0 2.0 40.0 62-63 29.817197717731744 34.5 28.0 38.0 2.0 40.0 64-65 29.16743293825401 34.0 26.5 38.0 2.0 40.0 66-67 28.48933098274142 34.0 26.0 37.0 2.0 39.5 68-69 27.65684998097303 33.5 24.5 36.5 2.0 39.0 70-71 26.828862701514957 32.5 22.5 36.0 2.0 39.0 72-73 25.9512339397562 32.0 19.5 35.0 2.0 38.0 74-75 25.05427087385513 31.0 15.5 35.0 2.0 37.5 76-77 24.12785396967906 30.5 7.5 34.5 2.0 36.5 78-79 23.16043757793041 30.0 3.5 34.0 2.0 36.0 80-81 22.22593209459398 29.0 2.0 34.0 2.0 35.5 82-83 21.238027227017447 28.5 2.0 34.0 2.0 35.0 84-85 20.280363442326504 26.5 2.0 33.0 2.0 35.0 86-87 19.31219973537691 25.5 2.0 33.0 2.0 35.0 88-89 18.201966735171823 24.0 2.0 32.0 2.0 34.0 90-91 17.046941558469058 19.0 2.0 31.5 2.0 34.0 92-93 15.890867176028802 11.0 2.0 31.0 2.0 34.0 94-95 14.627339122291076 2.0 2.0 31.0 2.0 34.0 96-97 13.199017857520976 2.0 2.0 30.0 2.0 34.0 98-99 11.47145924447507 2.0 2.0 28.5 2.0 34.0 100-101 8.074180070747653 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6231140550712162 1101 2 0.5784810458500402 1101 3 0.4917842790316165 1101 4 0.46933069049781295 1101 5 0.46698613119360033 1101 6 0.5492966599191007 1101 7 0.4357016816610155 1101 8 0.5534063947858385 1101 9 0.5179058600563167 1101 10-11 0.6662039413968657 1101 12-13 0.6003238966449871 1101 14-15 0.7941639143196824 1101 16-17 0.8268283342084928 1101 18-19 0.758007700451472 1101 20-21 0.9060863045824945 1101 22-23 0.9382360534484562 1101 24-25 0.8569663959610523 1101 26-27 0.9472138094339044 1101 28-29 0.9739732216746404 1101 30-31 1.0350447886992882 1101 32-33 1.0633445026598736 1101 34-35 1.1256867792630416 1101 36-37 1.3483781071102001 1101 38-39 1.4100253163773857 1101 40-41 1.383044263721466 1101 42-43 1.5617378345125275 1101 44-45 1.47873226107167 1101 46-47 1.5438296668343838 1101 48-49 1.4665966925981948 1101 50-51 1.5885136623331917 1101 52-53 1.7668709336703792 1101 54-55 1.805970804661218 1101 56-57 2.0565446618152983 1101 58-59 1.816736336103645 1101 60-61 1.9028559381319106 1101 62-63 1.795424528942533 1101 64-65 1.8642004963298398 1101 66-67 2.034561740970595 1101 68-69 2.2320828032592566 1101 70-71 2.542542238894356 1101 72-73 2.5352391434654074 1101 74-75 2.599121404155632 1101 76-77 2.8224204939385658 1101 78-79 2.8629738596693315 1101 80-81 3.1843079300334374 1101 82-83 3.272452959276599 1101 84-85 3.264138941660409 1101 86-87 3.4898277455725513 1101 88-89 3.82287907808146 1101 90-91 3.7382696108936635 1101 92-93 3.5592077627183283 1101 94-95 3.3634304063913163 1101 96-97 3.4628728808473532 1101 98-99 3.236727335624394 1101 100-101 2.476937862083666 1102 1 0.5923384329964598 1102 2 0.4667825560076828 1102 3 0.35179104023394814 1102 4 0.231224408759239 1102 5 0.4077543637207981 1102 6 0.5001478080512314 1102 7 0.4268802340055444 1102 8 0.4616796242154493 1102 9 0.39183342795005416 1102 10-11 0.5618494570631754 1102 12-13 0.5037591794237031 1102 14-15 0.5692249962263034 1102 16-17 0.6724673327404531 1102 18-19 0.7588579398198405 1102 20-21 0.7044121540299315 1102 22-23 0.6105624834661185 1102 24-25 0.6987706710973214 1102 26-27 0.7124745752984509 1102 28-29 0.664866422200376 1102 30-31 0.5764640076505927 1102 32-33 0.7671922083949028 1102 34-35 0.6347635116393846 1102 36-37 0.7132754713318121 1102 38-39 0.8273531562770984 1102 40-41 0.9244717394405981 1102 42-43 1.2053844593899115 1102 44-45 1.117314008006403 1102 46-47 1.032754588927368 1102 48-49 0.9366717772560307 1102 50-51 1.0503258515035299 1102 52-53 1.07953520570128 1102 54-55 1.0373363961651556 1102 56-57 1.277335878276066 1102 58-59 1.4367248285580487 1102 60-61 1.2936690635885633 1102 62-63 1.3037390422112658 1102 64-65 1.5721426761061572 1102 66-67 1.650771126371069 1102 68-69 1.7390759221001098 1102 70-71 1.8114315686057871 1102 72-73 1.8595230765741668 1102 74-75 1.925101609723189 1102 76-77 2.0504326433310176 1102 78-79 2.5475553064523844 1102 80-81 2.7854350567353556 1102 82-83 2.9438663154034543 1102 84-85 2.8790833798444773 1102 86-87 2.9027965313551256 1102 88-89 3.0540528425392424 1102 90-91 3.1017099931352803 1102 92-93 3.2379970609086417 1102 94-95 3.1037208369012674 1102 96-97 2.9595249432477466 1102 98-99 2.660598897706201 1102 100-101 1.9756941020450718 1103 1 0.3877459593028618 1103 2 0.39727123087838123 1103 3 0.3271343610828872 1103 4 0.28890231138095857 1103 5 0.3423985584773561 1103 6 0.34683944350690865 1103 7 0.41512706831979784 1103 8 0.4214442042047466 1103 9 0.4615587712709015 1103 10-11 0.544224167246874 1103 12-13 0.4577898553644957 1103 14-15 0.539900045272141 1103 16-17 0.6053369599921012 1103 18-19 0.7003685515551723 1103 20-21 0.6110021308446321 1103 22-23 0.6866370331361722 1103 24-25 0.7299103179673097 1103 26-27 0.7216684401102995 1103 28-29 0.7837936560152485 1103 30-31 0.6897063832525205 1103 32-33 0.8935030698181237 1103 34-35 0.822653648967723 1103 36-37 0.9670520006701437 1103 38-39 0.996971490502176 1103 40-41 1.2022450585060511 1103 42-43 1.3690281191009817 1103 44-45 1.4997422227379928 1103 46-47 1.5383368967568671 1103 48-49 1.3736345024210053 1103 50-51 1.6782463080756713 1103 52-53 1.6850105044350023 1103 54-55 1.678071191956132 1103 56-57 1.8754676778123702 1103 58-59 1.9991690946540004 1103 60-61 2.1350236203925554 1103 62-63 2.287812521790361 1103 64-65 2.316845725865388 1103 66-67 2.406897397104082 1103 68-69 2.5507800419503006 1103 70-71 2.3955540940204507 1103 72-73 2.7949252524257773 1103 74-75 2.5824807786192068 1103 76-77 2.719868170350818 1103 78-79 3.0654258254465 1103 80-81 3.0255902199242293 1103 82-83 3.1088832585102892 1103 84-85 3.3359810078095222 1103 86-87 3.4236142591953254 1103 88-89 3.6738272316956575 1103 90-91 3.6399835797234736 1103 92-93 3.5561573962038064 1103 94-95 3.4247213719467453 1103 96-97 3.4958010267148403 1103 98-99 3.5328567896270133 1103 100-101 2.6045999340407917 1104 1 0.43085685372082594 1104 2 0.4135378457500991 1104 3 0.4084125071257638 1104 4 0.37761324007720987 1104 5 0.3736802342779768 1104 6 0.43653945940025807 1104 7 0.3869235489059406 1104 8 0.40476411690836755 1104 9 0.5235180057778166 1104 10-11 0.5579251484440277 1104 12-13 0.5948228844591412 1104 14-15 0.7324274254330803 1104 16-17 0.673665142144209 1104 18-19 0.7184965001769967 1104 20-21 0.6189877830400476 1104 22-23 0.7991890215600748 1104 24-25 0.899598041998388 1104 26-27 0.8727008281858275 1104 28-29 0.7945631450913098 1104 30-31 0.7670338432199699 1104 32-33 0.8047628365822987 1104 34-35 0.8120992957948872 1104 36-37 1.0191968570203258 1104 38-39 0.9905472370270587 1104 40-41 0.9295877417335063 1104 42-43 1.0935289226399547 1104 44-45 1.0100716246384707 1104 46-47 1.0711989413662124 1104 48-49 1.097396510675729 1104 50-51 1.2334803159136243 1104 52-53 1.3116374490578302 1104 54-55 1.5877471450945428 1104 56-57 1.53987751741791 1104 58-59 1.5927802991998732 1104 60-61 1.6512520591410684 1104 62-63 1.5532404888726319 1104 64-65 1.509244043360109 1104 66-67 1.6409814929217177 1104 68-69 1.8798155177144515 1104 70-71 1.999697472780067 1104 72-73 2.279811282777164 1104 74-75 2.0977168269213955 1104 76-77 2.3303795529701397 1104 78-79 2.499121685663564 1104 80-81 2.899336353706957 1104 82-83 3.0115135698047197 1104 84-85 2.9897111595287065 1104 86-87 3.073311237986953 1104 88-89 3.0671961287539524 1104 90-91 2.916694502379496 1104 92-93 2.8260229954868326 1104 94-95 3.048958746078796 1104 96-97 3.0692050760076235 1104 98-99 2.8721187002943207 1104 100-101 2.290857180897703 1105 1 0.5042454340783387 1105 2 0.4172547866759224 1105 3 0.3623571277787043 1105 4 0.2173221830171883 1105 5 0.24186150952422736 1105 6 0.2819326914843856 1105 7 0.3191286742419095 1105 8 0.3019273007745653 1105 9 0.39219971018536626 1105 10-11 0.4149415249550259 1105 12-13 0.39720451875331975 1105 14-15 0.46528475161387917 1105 16-17 0.41678135493894075 1105 18-19 0.4746575735375984 1105 20-21 0.4188065273314052 1105 22-23 0.46633449280937356 1105 24-25 0.5420273302922141 1105 26-27 0.4571282453097396 1105 28-29 0.5171884488677989 1105 30-31 0.5372909004034341 1105 32-33 0.5960229101058729 1105 34-35 0.5008708554332131 1105 36-37 0.6345841878590548 1105 38-39 0.6987371798662423 1105 40-41 0.7954112274468557 1105 42-43 0.933534215555305 1105 44-45 0.7995740683934827 1105 46-47 1.1275222212686273 1105 48-49 0.8972396325721874 1105 50-51 0.8613492025852878 1105 52-53 0.856421241014278 1105 54-55 1.0977965352769807 1105 56-57 1.3024464818403665 1105 58-59 1.2794865117592735 1105 60-61 1.3579614954056147 1105 62-63 1.335665700911175 1105 64-65 1.4427898925732983 1105 66-67 1.643929851030066 1105 68-69 1.8859501487001218 1105 70-71 1.91109757766149 1105 72-73 2.0869077412750663 1105 74-75 2.2293037202065413 1105 76-77 2.322995181190791 1105 78-79 2.682075167953389 1105 80-81 2.6855738857164404 1105 82-83 2.9191014879261594 1105 84-85 3.140953729132537 1105 86-87 3.150325776186751 1105 88-89 3.2432213535807435 1105 90-91 3.0655661389693485 1105 92-93 3.3162390947414284 1105 94-95 3.308541431673852 1105 96-97 3.3452804769615057 1105 98-99 3.0113971054216986 1105 100-101 2.2718040621405784 1106 1 0.3320634111200427 1106 2 0.17314411924717632 1106 3 0.12794794031361434 1106 4 0.17684357632669645 1106 5 0.24231046250729094 1106 6 -0.07209187910063974 1106 7 0.26511874906308464 1106 8 0.055067444351386996 1106 9 0.1869701337227383 1106 10-11 0.30583104545406314 1106 12-13 0.38013510341608736 1106 14-15 0.4584659541702152 1106 16-17 0.041560215785558796 1106 18-19 0.4764265571593711 1106 20-21 0.5306791563632629 1106 22-23 0.38225664145895877 1106 24-25 0.5416758405433484 1106 26-27 0.43653032392058577 1106 28-29 0.5835244922493033 1106 30-31 0.367059438182757 1106 32-33 0.4793193707129717 1106 34-35 0.3881708257535834 1106 36-37 0.41324803431299273 1106 38-39 0.5012123831198494 1106 40-41 0.6275567276134026 1106 42-43 0.2326636563211153 1106 44-45 0.8344753674280483 1106 46-47 0.803493645877829 1106 48-49 0.7790101196161956 1106 50-51 0.5493091234223328 1106 52-53 0.872102323685656 1106 54-55 0.8115947112110824 1106 56-57 1.2487275207051347 1106 58-59 1.2009591397542891 1106 60-61 1.3631865356804838 1106 62-63 1.3545731795295275 1106 64-65 1.347919802510532 1106 66-67 1.5580687594677656 1106 68-69 1.9049931945298724 1106 70-71 1.7625064527776537 1106 72-73 1.6852157432398833 1106 74-75 1.994727082610357 1106 76-77 2.2595026795091613 1106 78-79 2.5079568408475943 1106 80-81 2.395195787986218 1106 82-83 2.640936362915305 1106 84-85 2.637606540985729 1106 86-87 2.1609137568444474 1106 88-89 2.142712522919222 1106 90-91 2.404837860504447 1106 92-93 2.5598945881296107 1106 94-95 2.6930793820686922 1106 96-97 2.6535407452705293 1106 98-99 2.5447695795751475 1106 100-101 1.8397984061624655 1107 1 0.3007838143812762 1107 2 0.27358487136202214 1107 3 0.3204015527782005 1107 4 0.2577565261467498 1107 5 0.27177621996214185 1107 6 0.20871265849502407 1107 7 0.28504352548806366 1107 8 0.33467980012081 1107 9 0.36851048402721887 1107 10-11 0.40610807092891577 1107 12-13 0.34756483697994867 1107 14-15 0.5057876130384571 1107 16-17 0.4513032606778893 1107 18-19 0.6065180778322556 1107 20-21 0.5944747378598336 1107 22-23 0.4881324883035134 1107 24-25 0.524964766174179 1107 26-27 0.478305050169638 1107 28-29 0.6462959006409719 1107 30-31 0.6387966685123772 1107 32-33 0.767367930523065 1107 34-35 0.7040278515911567 1107 36-37 0.7344147150853431 1107 38-39 0.8294112490232024 1107 40-41 0.7898084424610374 1107 42-43 1.0156615011747618 1107 44-45 0.8356365671362624 1107 46-47 0.7340880340338956 1107 48-49 0.7211479915527477 1107 50-51 1.0100506237427354 1107 52-53 1.0151826565393982 1107 54-55 1.0312154850486728 1107 56-57 1.3191409329899706 1107 58-59 1.3655566795222285 1107 60-61 1.3296257230724216 1107 62-63 1.5005779220605717 1107 64-65 1.6152061871039045 1107 66-67 1.7759619653663563 1107 68-69 1.6903877455057845 1107 70-71 1.973814573215975 1107 72-73 1.9242488291192963 1107 74-75 2.1714780508645184 1107 76-77 2.1222622240166125 1107 78-79 2.608777564710774 1107 80-81 2.588447756736091 1107 82-83 2.776613984657395 1107 84-85 2.6899611567671613 1107 86-87 2.603576057803231 1107 88-89 2.940749037486359 1107 90-91 2.9149395423044915 1107 92-93 3.0482445560895695 1107 94-95 2.932689793269411 1107 96-97 2.618663822364116 1107 98-99 2.292248944980768 1107 100-101 1.6890812331018967 1108 1 0.2935137460611372 1108 2 0.2133854376848454 1108 3 0.1306038699728056 1108 4 0.11285553011575189 1108 5 0.09951392752734023 1108 6 0.17472263610611805 1108 7 0.08831186433852878 1108 8 0.1859253835332808 1108 9 0.19783644901234254 1108 10-11 0.28761016244022386 1108 12-13 0.23390547073862678 1108 14-15 0.2763512974432416 1108 16-17 0.3162345784966192 1108 18-19 0.2925068343570132 1108 20-21 0.29740600153251506 1108 22-23 0.3008998143985764 1108 24-25 0.3917216308680054 1108 26-27 0.44402827799186895 1108 28-29 0.46314965152043897 1108 30-31 0.4821147816795488 1108 32-33 0.487252314289762 1108 34-35 0.585614486231556 1108 36-37 0.7147979858143074 1108 38-39 0.6359675987208178 1108 40-41 0.687679741786809 1108 42-43 0.7209290547473088 1108 44-45 0.7207330432216494 1108 46-47 0.9262851637806477 1108 48-49 1.0851998766042001 1108 50-51 1.0014291068715977 1108 52-53 1.178790031359494 1108 54-55 1.2569975830831375 1108 56-57 1.3744989809511914 1108 58-59 1.4825689758017262 1108 60-61 1.5757523982358528 1108 62-63 1.4509660034577827 1108 64-65 1.6124139410104341 1108 66-67 1.8832418696848414 1108 68-69 2.0434056909163125 1108 70-71 1.9918195764391058 1108 72-73 1.970735695658572 1108 74-75 2.1034035708908654 1108 76-77 2.0207751460193855 1108 78-79 2.391395713633557 1108 80-81 2.5963342169557286 1108 82-83 2.8279496059016154 1108 84-85 2.8017536457168823 1108 86-87 2.854030972726651 1108 88-89 2.802977338056529 1108 90-91 2.797806578830329 1108 92-93 2.795973706015813 1108 94-95 2.8176615572259536 1108 96-97 2.8329916279945344 1108 98-99 2.7494726847875324 1108 100-101 1.9405841864645268 1109 1 0.6916900189272255 1109 2 0.6397038280083542 1109 3 0.44787181090294226 1109 4 0.4565881082553105 1109 5 0.39767227536452765 1109 6 0.5255471207170572 1109 7 0.4849536123980229 1109 8 0.4313957109386024 1109 9 0.5492080250760694 1109 10-11 0.6009621295008785 1109 12-13 0.5997013131104652 1109 14-15 0.7984980042418144 1109 16-17 0.8424991047754631 1109 18-19 0.8479905810705759 1109 20-21 0.8539236176548926 1109 22-23 0.8661678691949035 1109 24-25 0.6582619624054189 1109 26-27 0.5824804005955073 1109 28-29 0.8925742314053622 1109 30-31 0.712083028135801 1109 32-33 0.8367607548433824 1109 34-35 0.9284012188635202 1109 36-37 1.0532637189857965 1109 38-39 1.0778340612586135 1109 40-41 0.8667603434806566 1109 42-43 1.0759664548975607 1109 44-45 1.1529485456584325 1109 46-47 1.3231227527886418 1109 48-49 1.5235719815229984 1109 50-51 1.399116911580407 1109 52-53 1.4118887529001718 1109 54-55 1.6990115379182917 1109 56-57 1.8344756828615267 1109 58-59 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0.1987167924630029 2316 96-97 0.32180960850728546 2316 98-99 0.13869889644231392 2316 100-101 0.2796298460321296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9984.0 3 3723.0 4 3979.0 5 4702.0 6 5754.0 7 7299.0 8 9824.0 9 12813.0 10 16656.0 11 21103.0 12 26189.0 13 31289.0 14 36178.0 15 41932.0 16 48759.0 17 56523.0 18 55327.0 19 54725.0 20 58647.0 21 64478.0 22 71232.0 23 79176.0 24 88148.0 25 96897.0 26 107862.0 27 119757.0 28 133606.0 29 148305.0 30 164945.0 31 186125.0 32 208912.0 33 238566.0 34 267843.0 35 292162.0 36 285692.0 37 197565.0 38 48498.0 39 659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71115362424463 18.239682973490023 14.533661291791928 23.515502110473413 2 15.51671498455754 19.753247425222757 39.93374583831213 24.796291751907575 3 19.191170018004932 23.549089918750852 29.698727550572677 27.56101251267154 4 12.868517336028736 16.19660512227673 36.35799648346593 34.5768810582286 5 13.727422669303305 37.37605188847296 33.921138747120274 14.975386695103463 6 32.926133517593215 35.62417279025094 16.99119468987712 14.458499002278732 7 29.203391733338417 32.74579278786113 19.94917423258266 18.1016412462178 8 27.282724510969576 34.12704729147608 19.48725832178612 19.10296987576822 9 26.841532548221213 16.522335388810852 19.69215648419538 36.94397557877256 10-11 25.22284798162081 26.228867759464286 27.070979998529328 21.477304260385576 12-13 26.45876750721469 23.515792897640235 27.269127920390634 22.756311674754436 14-15 22.677886011165384 25.05862046480934 25.700037806966016 26.563455717059263 16-17 22.292171030029056 28.266833640155692 26.78770227545754 22.653293054357707 18-19 22.49463494558545 26.345582879027763 29.175245332572857 21.984536842813927 20-21 22.756602775009842 25.62375386153516 29.527138395544057 22.09250496791094 22-23 22.474668654916204 25.696788818638144 29.530997735229253 22.2975447912164 24-25 22.34214710890337 25.85901741889182 29.563423883003104 22.235411589201707 26-27 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27.62181360536809 22.488972790473944 52-53 23.19137733115778 26.491388116149366 27.826579150417963 22.490655402274893 54-55 22.316221726157615 26.982311387465955 28.1094230864059 22.592043799970536 56-57 22.54417465690589 27.027506974269134 27.703795155017623 22.724523213807352 58-59 22.496667497050925 26.438875172675306 27.76274061291434 23.301716717359433 60-61 21.82852956017614 26.84512868481206 28.23575536756458 23.090586387447225 62-63 22.23887485230833 26.792580920512954 28.358328161320216 22.610216065858495 64-65 21.872930591920667 26.970394134792674 28.00952627287977 23.147149000406884 66-67 21.725620890733303 26.965108450835668 28.083641221890154 23.225629436540878 68-69 22.724482723883746 26.33820814349324 28.15857053701969 22.778738595603322 70-71 22.32328564880854 26.684551514867316 27.547426109659785 23.44473672666436 72-73 22.305370290928828 26.843967082561637 27.59526447277583 23.255398153733704 74-75 22.369307546837028 27.245510521862222 27.504824606385032 22.880357324915725 76-77 22.563218531629044 26.71452705385451 27.60961400556894 23.112640408947513 78-79 22.638946263256 27.019073937126297 27.4458888078967 22.896090991721 80-81 22.95646083880595 27.05917232710301 27.032353165848455 22.95201366824259 82-83 22.757172898956345 27.126517179651554 27.179793749185045 22.936516172207057 84-85 22.649257653942932 27.25754352316206 27.190626117905627 22.902572704989385 86-87 23.098685836479614 27.285417271042334 26.602395883782 23.013501008696053 88-89 22.808185255901943 27.312726283684825 26.424049914849114 23.455038545564122 90-91 22.720732346593827 27.313844131265046 26.598804687151333 23.36661883498979 92-93 23.04424549590091 26.767491890004774 26.8597324026793 23.328530211415014 94-95 22.67623069176457 26.45304838928543 27.13171375636939 23.73900716258061 96-97 23.131189375079135 25.97637881157645 27.024057923088407 23.868373890256006 98-99 22.82639672105755 26.37855412384857 26.458180051405915 24.336869103687963 100-101 23.64140866599964 25.344612102875487 26.971658252616553 24.042320978508318 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 541.0 1 411.5 2 392.5 3 501.5 4 433.0 5 430.0 6 737.0 7 1561.0 8 2527.0 9 2881.5 10 2586.5 11 2197.0 12 2120.5 13 2306.5 14 2514.5 15 2775.0 16 3091.5 17 3432.0 18 3928.0 19 4528.0 20 5280.5 21 6305.0 22 7816.0 23 9611.5 24 11606.0 25 14427.5 26 18348.5 27 23127.0 28 27556.0 29 31905.5 30 37443.0 31 46762.0 32 59501.5 33 63469.5 34 61642.0 35 64553.0 36 69883.5 37 76186.5 38 81743.0 39 85914.5 40 90672.0 41 96898.5 42 101223.0 43 103954.5 44 107268.0 45 113708.5 46 118736.5 47 121485.5 48 122591.0 49 120401.0 50 118192.0 51 115859.0 52 113645.0 53 114270.0 54 116751.5 55 112903.0 56 104865.0 57 96881.5 58 89941.5 59 79101.0 60 63480.5 61 52861.5 62 45983.0 63 35857.5 64 25546.5 65 19211.0 66 14680.0 67 11164.0 68 8576.0 69 6669.0 70 5211.5 71 3849.5 72 2822.0 73 2161.5 74 1639.0 75 1294.5 76 970.5 77 656.5 78 430.5 79 275.5 80 158.0 81 96.5 82 65.0 83 41.5 84 30.0 85 18.0 86 9.0 87 4.5 88 3.5 89 4.0 90 3.0 91 2.5 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3851978048504553 2 0.029795809468957 3 0.03581547046826913 4 0.04368035418596336 5 0.06452229603785307 6 0.054600442732454194 7 0.03663220839279891 8 0.038870675297065733 9 0.03533147762410333 10-11 0.03711620123696471 12-13 0.028631201687683048 14-15 0.026846478074821663 16-17 0.021734303658320412 18-19 0.02666498075825949 20-21 0.03403074685540774 22-23 0.03280563996861306 24-25 0.042485496851929046 26-27 0.0364809606289971 28-29 0.0313234118833553 30-31 0.03407612118454829 32-33 0.028631201687683048 34-35 0.030037805891039902 36-37 0.03226114801892654 38-39 0.01462565875963524 40-41 0.01267456260659186 42-43 0.013294678438179292 44-45 0.011494830048937726 46-47 0.019934455269078846 48-49 0.017665738812051666 50-51 0.011313332732375551 52-53 0.009513484343133987 54-55 0.007895133270454597 56-57 0.008787495076885287 58-59 0.004098814399029111 60-61 0.0071993935569662606 62-63 0.011600703483598995 64-65 0.007229643109726623 66-67 0.0036148215548633114 68-69 0.00608016010483285 70-71 0.012977058134195486 72-73 0.010647842571647578 74-75 0.011585578707218814 76-77 0.0053692956149643326 78-79 0.009150489710009637 80-81 0.01093521332287102 82-83 0.013340052767319834 84-85 0.00806150581063659 86-87 0.0024502137735893576 88-89 0.01588101519919028 90-91 0.02545499864784499 92-93 0.010436095702325041 94-95 0.020282325125823016 96-97 0.01886059614608598 98-99 0.011434330943417002 100-101 0.12446178483251126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3305834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.9977737316443 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42499713123672 38.465069374087314 2 13.216080496565471 13.479813655660863 3 4.685502983858317 7.168506629692523 4 2.235799568891786 4.560832020946048 5 1.2178084329310566 3.1052759455553174 6 0.7509452424748162 2.2977921336351264 7 0.4875103723680953 1.7403360563300452 8 0.33873949036125584 1.3819967906732666 9 0.2642229895325108 1.2127305811389846 >10 1.2393847202123447 11.31229575380998 >50 0.08280043071911855 2.898051329383862 >100 0.04701138037452046 4.658420589420025 >500 0.004837257133483768 1.6688077340297136 >1k 0.003881749549821057 4.366401400563583 >5k 4.1803456690380603E-4 1.3586491796089977 >10k+ 5.9719223843400875E-5 0.3250208254642851 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10672 0.32282322705858796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8167 0.24704809739388003 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6632 0.2006150339067237 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6581 0.1990723067159452 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6439 0.19477687022397375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5858 0.17720188007020315 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5633 0.17039573069912162 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5301 0.1603528791826813 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4940 0.14943279063619044 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4921 0.14885804913374356 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4711 0.14250564305406743 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4413 0.13349127633147945 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4306 0.13025457418612066 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3962 0.11984872803655598 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3682 0.1113788532636545 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3456 0.10454245433981259 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3392 0.10260648296314939 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3332 0.10079150979752764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.0499105520724873E-5 2 0.0 0.0 0.0 0.0 6.0499105520724873E-5 3 0.0 0.0 0.0 0.0 9.074865828108732E-5 4 0.0 0.0 0.0 0.0 9.074865828108732E-5 5 0.0 0.0 0.0 0.0 9.074865828108732E-5 6 0.0 0.0 0.0 0.0 1.8149731656217464E-4 7 0.0 0.0 0.0 0.0 2.1174686932253707E-4 8 0.0 0.0 0.0 0.0 2.1174686932253707E-4 9 0.0 0.0 0.0 3.0249552760362437E-5 2.1174686932253707E-4 10-11 0.0 0.0 0.0 3.0249552760362437E-5 2.1174686932253707E-4 12-13 0.0 0.0 0.0 3.0249552760362437E-5 3.024955276036244E-4 14-15 3.0249552760362437E-5 0.0 0.0 3.0249552760362437E-5 3.932441858847117E-4 16-17 3.0249552760362437E-5 0.0 0.0 6.0499105520724873E-5 4.2349373864507413E-4 18-19 4.537432914054365E-5 0.0 0.0 1.0587343466126853E-4 4.5374329140543656E-4 20-21 6.0499105520724873E-5 0.0 0.0 1.512477638018122E-4 6.352406079676112E-4 22-23 6.0499105520724873E-5 0.0 0.0 2.5712119846308073E-4 6.503653843477924E-4 24-25 9.074865828108732E-5 0.0 0.0 5.747415024468863E-4 6.654901607279736E-4 26-27 9.074865828108732E-5 0.0 0.0 0.00124023166317486 6.806149371081548E-4 28-29 1.8149731656217464E-4 0.0 0.0 0.003887067529706573 7.56238819009061E-4 30-31 1.8149731656217464E-4 0.0 0.0 0.01265943783021168 8.016131481496046E-4 32-33 1.8149731656217464E-4 0.0 0.0 0.030839419039189506 9.074865828108731E-4 34-35 1.8149731656217464E-4 0.0 0.0 0.057655647561250814 9.377361355712355E-4 36-37 1.8149731656217464E-4 0.0 0.0 0.10056463815182493 9.377361355712355E-4 38-39 1.8149731656217464E-4 0.0 0.0 0.17228632774664426 9.67985688331598E-4 40-41 1.8149731656217464E-4 0.0 0.0 0.2520090240465795 9.67985688331598E-4 42-43 1.8149731656217464E-4 0.0 0.0 0.33431805710752566 9.67985688331598E-4 44-45 1.8149731656217464E-4 0.0 0.0 0.4394957520553059 9.831104647117792E-4 46-47 1.8149731656217464E-4 0.0 0.0 0.5641692837571397 9.982352410919605E-4 48-49 1.8149731656217464E-4 0.0 0.0 0.6959968346867991 9.982352410919605E-4 50-51 1.8149731656217464E-4 0.0 0.0 0.8470328516192889 0.0010587343466126854 52-53 1.8149731656217464E-4 0.0 0.0 0.9901434857285636 0.0011494830048937726 54-55 2.419964220828995E-4 0.0 0.0 1.1419659910328226 0.0011494830048937726 56-57 2.7224597484326195E-4 0.0 0.0 1.3199543594747953 0.0011948573340343164 58-59 2.7224597484326195E-4 0.0 0.0 1.5102089215610945 0.0012099821104144975 60-61 2.7224597484326195E-4 0.0 0.0 1.7198080726376461 0.00124023166317486 62-63 2.8737075122344316E-4 0.0 0.0 1.9268208869531862 0.00124023166317486 64-65 3.024955276036244E-4 0.0 0.0 2.1418800823029835 0.00124023166317486 66-67 3.327450803639868E-4 0.0 0.0 2.3994550240574695 0.0012553564395550412 68-69 3.327450803639868E-4 0.0 0.0 2.680851488610741 0.0012704812159352223 70-71 3.629946331243493E-4 0.0 0.0 2.9718824357181877 0.0012856059923154035 72-73 3.781194095045305E-4 3.0249552760362437E-5 0.0 3.2802312517809424 0.001376354650596491 74-75 4.083689622648929E-4 3.0249552760362437E-5 0.0 3.5788397118548603 0.0014973528616379407 76-77 4.5374329140543656E-4 3.0249552760362437E-5 0.0 3.9026762989309205 0.0015578519671586655 78-79 4.5374329140543656E-4 3.0249552760362437E-5 0.0 4.274382803250253 0.0016183510726793904 80-81 4.5374329140543656E-4 4.537432914054365E-5 0.0 4.6609267131985455 0.001648600625439753 82-83 4.5374329140543656E-4 6.0499105520724873E-5 0.0 5.059283073499758 0.0016637254018199341 84-85 4.688680677856178E-4 6.0499105520724873E-5 0.0 5.461481127001537 0.0016637254018199341 86-87 4.83992844165799E-4 6.0499105520724873E-5 0.0 5.871014697047704 0.00173934928372084 88-89 4.83992844165799E-4 6.0499105520724873E-5 0.0 6.302932936136539 0.0018300979420019276 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5875 0.0 55.595062 1 GTATCAA 11100 0.0 44.116493 1 TATCAAC 14220 0.0 34.060028 2 ATCAACG 14365 0.0 33.65005 3 TCAACGC 14555 0.0 33.210785 4 CAACGCA 14930 0.0 32.344788 5 AACGCAG 15470 0.0 31.208193 6 TGGTATC 2640 0.0 28.446154 2 AGGTAAG 2145 0.0 27.919888 2 ACGCAGA 17575 0.0 27.275679 7 CGCAGAG 17665 0.0 27.05603 8 TAAGGTG 2555 0.0 26.97413 5 GTGGTAT 3085 0.0 26.120714 1 GTACATG 12790 0.0 25.313532 1 TACATGG 12690 0.0 24.944971 2 GTAAGGT 2695 0.0 24.514692 4 GGTAAGG 2590 0.0 24.407442 3 AAGGTAA 2315 0.0 24.304377 1 GCAGAGT 19420 0.0 24.046461 9 ACATGGG 13155 0.0 23.340601 3 >>END_MODULE