FastQCFastQC Report
Thu 26 May 2016
SRR938356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305834
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108990.3296898755351902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88110.26652880937155343No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA68050.2058482065342664No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67240.20339799276067705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63910.19332489169147635No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63070.19078392925960588No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC61630.1864279936621137No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA56230.17009323517151798No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53300.16123011621273178No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47770.14450211353625136No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA44180.13364252409528124No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG42780.1294075867088305No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA42720.12922608939226835No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40850.12356942302608055No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39050.11812450352921532No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT34970.10578268600298746No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG34540.10448195523429185No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34490.10433070747049006No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA33980.10278798027971156No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG33160.10030751695336185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.053.5187231
GTATCAA112100.042.3845441
TATCAAC143950.032.867662
ATCAACG144100.032.797013
TCAACGC147700.031.997144
CAACGCA149250.031.569375
AACGCAG154650.030.528476
TAAGGTG25050.028.8206945
AGGTAAG23050.027.2034592
ACGCAGA177800.026.2597377
CGCAGAG179100.025.9895698
GGTAAGG26850.025.1199763
GTAAGGT27450.025.089634
TGGTATC30300.024.4569952
GTGGTAT32850.023.7203771
AAGGTAA26100.023.1193871
GCAGAGT201950.022.7666999
GTACATG130000.022.5138851
GAGTACT118150.022.21094912-13
TACATGG128450.022.1520462
Produced by FastQC (version 0.11.5)