##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3305834 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835778203019267 31.0 31.0 34.0 30.0 34.0 2 32.092038801706316 33.0 31.0 34.0 30.0 34.0 3 32.144513003375245 33.0 31.0 34.0 30.0 34.0 4 35.79570541049551 37.0 35.0 37.0 35.0 37.0 5 35.69213517678141 37.0 35.0 37.0 33.0 37.0 6 35.7263377410965 37.0 35.0 37.0 33.0 37.0 7 35.6482515456009 37.0 35.0 37.0 33.0 37.0 8 35.61312122750265 37.0 35.0 37.0 33.0 37.0 9 37.164047257061306 39.0 37.0 39.0 33.0 39.0 10-11 37.09638838489773 39.0 37.0 39.0 33.0 39.0 12-13 36.97872080086296 39.0 37.0 39.0 33.0 39.0 14-15 38.00296838861237 40.0 37.0 41.0 33.0 41.0 16-17 38.08926627894806 40.0 37.0 41.0 33.0 41.0 18-19 38.235158359433655 40.0 38.0 41.0 33.0 41.0 20-21 38.189753931988115 40.0 37.5 41.0 33.0 41.0 22-23 38.071621109831895 40.0 37.0 41.0 33.0 41.0 24-25 37.940014834380676 40.0 37.0 41.0 33.0 41.0 26-27 37.72269176250229 40.0 37.0 41.0 32.0 41.0 28-29 37.58302564496584 39.5 37.0 41.0 32.0 41.0 30-31 37.31036903244386 39.0 36.0 41.0 31.5 41.0 32-33 37.17758181445288 39.0 36.0 41.0 31.0 41.0 34-35 37.03585373615251 39.0 36.0 41.0 31.0 41.0 36-37 36.88827221814526 39.0 35.5 41.0 30.5 41.0 38-39 36.663514259941664 39.0 35.0 41.0 30.0 41.0 40-41 36.42684251538341 39.0 35.0 40.0 30.0 41.0 42-43 36.150064401297826 38.5 35.0 40.0 30.0 41.0 44-45 35.84690792096639 38.0 35.0 40.0 29.0 41.0 46-47 35.407260013660704 38.0 34.5 40.0 27.5 41.0 48-49 34.96540963641853 38.0 34.0 40.0 26.0 41.0 50-51 34.54647253915351 38.0 33.0 40.0 24.5 41.0 52-53 33.939928320659774 37.0 33.0 40.0 23.5 41.0 54-55 33.5014185527767 37.0 32.0 40.0 22.5 41.0 56-57 33.0983523371107 36.5 32.0 39.0 21.5 40.5 58-59 32.5487769500828 36.0 31.0 39.0 19.0 40.0 60-61 32.052077630032244 35.5 31.0 39.0 17.5 40.0 62-63 31.55288241938343 35.0 30.0 38.5 13.5 40.0 64-65 30.901056889123893 35.0 29.0 38.0 8.0 40.0 66-67 30.24932755244214 34.0 28.5 37.5 7.0 40.0 68-69 29.52042510301485 34.0 27.5 37.0 3.5 39.0 70-71 28.73156395632691 33.5 26.0 36.0 2.0 39.0 72-73 28.162773448394567 33.0 26.0 36.0 2.0 38.5 74-75 27.460923174000875 32.0 25.0 35.0 2.0 38.0 76-77 26.312581635980514 30.5 24.0 34.0 2.0 36.0 78-79 26.800212896352328 32.0 25.0 35.0 2.0 36.0 80-81 26.43833038803521 32.0 24.0 35.0 2.0 36.0 82-83 25.771765158202136 31.5 22.0 34.5 2.0 36.0 84-85 25.00802187889652 31.0 19.0 34.0 2.0 35.0 86-87 24.122349458563257 30.5 14.5 34.0 2.0 35.0 88-89 23.14062049092604 30.0 6.5 34.0 2.0 35.0 90-91 22.284849753496395 29.0 2.0 34.0 2.0 35.0 92-93 21.237171315922097 29.0 2.0 33.5 2.0 35.0 94-95 20.074785818041683 27.0 2.0 33.0 2.0 34.5 96-97 18.713099024330923 25.0 2.0 32.5 2.0 34.0 98-99 16.927116878826943 12.5 2.0 32.0 2.0 34.0 100-101 12.650309876418477 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2998583970971822 1101 2 0.2750435817899515 1101 3 0.3301088076875871 1101 4 0.23081787548681376 1101 5 0.18730308380020944 1101 6 0.193610112744814 1101 7 0.20103243176747299 1101 8 0.27548604454515413 1101 9 0.3522049866374459 1101 10-11 0.3640319783342534 1101 12-13 0.3605569369441284 1101 14-15 0.6223665226431265 1101 16-17 0.46205441019585436 1101 18-19 0.4278143024121732 1101 20-21 0.4464246850547724 1101 22-23 0.41071778718725227 1101 24-25 0.4150024241046353 1101 26-27 0.5337050317448373 1101 28-29 0.6157260066010437 1101 30-31 0.6689038397812652 1101 32-33 0.5311448779757839 1101 34-35 0.4575908058637097 1101 36-37 0.5908130458948762 1101 38-39 0.5974247334463385 1101 40-41 0.678913966762984 1101 42-43 0.7728498537164583 1101 44-45 0.8248375284683291 1101 46-47 0.8511641730493054 1101 48-49 0.7939438301847659 1101 50-51 0.8843142043465448 1101 52-53 0.9630334839554138 1101 54-55 0.9605495395606951 1101 56-57 1.0892842865134966 1101 58-59 1.1743965029129413 1101 60-61 1.1768307590343383 1101 62-63 1.2916482593798086 1101 64-65 1.3371203135900522 1101 66-67 1.3287579824063585 1101 68-69 1.5057016647554669 1101 70-71 1.6629723317411376 1101 72-73 1.8748189587980768 1101 74-75 1.8828900787714709 1101 76-77 1.8374914935513935 1101 78-79 1.8032093240804663 1101 80-81 1.8222991142029663 1101 82-83 2.0280867328485463 1101 84-85 2.22730809278195 1101 86-87 2.19115779216294 1101 88-89 2.2873436966104457 1101 90-91 2.57459545091033 1101 92-93 2.835807176047286 1101 94-95 2.490331860174095 1101 96-97 2.5341742119360937 1101 98-99 2.7180987207014873 1101 100-101 2.2396598086019885 1102 1 0.11060725478114009 1102 2 0.14596599777214436 1102 3 0.13666769324203187 1102 4 0.1226710421130619 1102 5 0.12333795540227754 1102 6 0.19957606889670387 1102 7 0.21273055810433306 1102 8 0.17230822752031827 1102 9 0.2396846627852014 1102 10-11 0.27487638603578546 1102 12-13 0.2735553743897654 1102 14-15 0.33430752258444585 1102 16-17 0.17092051709867206 1102 18-19 0.23898586506520303 1102 20-21 0.11475805906610503 1102 22-23 0.2287165024549509 1102 24-25 0.19187755425259212 1102 26-27 0.2690670027339763 1102 28-29 0.24862247556053063 1102 30-31 0.29774919571286773 1102 32-33 0.2295097263706225 1102 34-35 0.45869446049609763 1102 36-37 0.41370194874699706 1102 38-39 0.5377302234699286 1102 40-41 0.5170538467957826 1102 42-43 0.5152374513241753 1102 44-45 0.5817926090548937 1102 46-47 0.6031535088364706 1102 48-49 0.5382933189142776 1102 50-51 0.7447217043997796 1102 52-53 0.781758794791557 1102 54-55 0.7014847769012249 1102 56-57 0.8151670206772508 1102 58-59 0.9169524554972881 1102 60-61 0.8895757812159601 1102 62-63 1.0554263382324613 1102 64-65 1.2010826378026955 1102 66-67 1.2258355539611188 1102 68-69 1.3353418514311208 1102 70-71 1.3356566588898566 1102 72-73 1.3956468373851614 1102 74-75 1.3927046753868204 1102 76-77 1.387935827097813 1102 78-79 1.4404820877037459 1102 80-81 1.5963423807049502 1102 82-83 1.7898359836377438 1102 84-85 1.825030626955364 1102 86-87 1.8159567606381835 1102 88-89 1.738014961448613 1102 90-91 1.9606266095633451 1102 92-93 1.8279723861820294 1102 94-95 1.7899691855421587 1102 96-97 1.8329979081327323 1102 98-99 1.6718193812636848 1102 100-101 1.6608092856993224 1103 1 0.20636256064880598 1103 2 0.2242520687548435 1103 3 0.24506790726022132 1103 4 0.14369832943248895 1103 5 0.0839764430070602 1103 6 0.18470180458423613 1103 7 0.18642415539343205 1103 8 0.19035709500739273 1103 9 0.23988976355209957 1103 10-11 0.22894273167516843 1103 12-13 0.271116458748601 1103 14-15 0.4419497555075793 1103 16-17 0.3206663704962054 1103 18-19 0.28759028810782894 1103 20-21 0.30936301715421166 1103 22-23 0.39255634550827523 1103 24-25 0.34898028298453454 1103 26-27 0.39485173610297863 1103 28-29 0.47022939548205756 1103 30-31 0.3318761798398526 1103 32-33 0.35262369105761593 1103 34-35 0.3791599500627143 1103 36-37 0.44315620235855135 1103 38-39 0.40801736454358917 1103 40-41 0.5382505651835103 1103 42-43 0.6613449954654342 1103 44-45 0.6281810520725557 1103 46-47 0.578113380425556 1103 48-49 0.3528064275285132 1103 50-51 0.47774738658277016 1103 52-53 0.48065749284756265 1103 54-55 0.6032682618307774 1103 56-57 0.7373491144885591 1103 58-59 0.9204168749729362 1103 60-61 0.8330571437984489 1103 62-63 0.8433878149579819 1103 64-65 1.014365141815528 1103 66-67 0.9704405120492261 1103 68-69 1.0886279224138171 1103 70-71 1.3122261256278662 1103 72-73 1.3172582160190132 1103 74-75 1.2944614080424266 1103 76-77 1.4010533587546696 1103 78-79 1.5479727244078667 1103 80-81 1.676255881685865 1103 82-83 1.8560612377843455 1103 84-85 2.0262032682965447 1103 86-87 1.8258996891204404 1103 88-89 1.7502095396544277 1103 90-91 2.11759557692557 1103 92-93 2.178311248318643 1103 94-95 1.8848951709175807 1103 96-97 2.038178931853082 1103 98-99 2.136363700864184 1103 100-101 1.6566582245257901 1104 1 0.23706560156341538 1104 2 0.22615030451774487 1104 3 0.24056491791404966 1104 4 0.13166563066276638 1104 5 0.08289958375907247 1104 6 0.08845607002277944 1104 7 0.09548570417562985 1104 8 0.16191177775012022 1104 9 0.17908515621782328 1104 10-11 0.23703559790263284 1104 12-13 0.1555104276572834 1104 14-15 0.27500212389951884 1104 16-17 0.27444065362505654 1104 18-19 0.32052514576798785 1104 20-21 0.3512405726893846 1104 22-23 0.40061617924347814 1104 24-25 0.2612964288514874 1104 26-27 0.46094206128238824 1104 28-29 0.4299844000933959 1104 30-31 0.41480028778325106 1104 32-33 0.5503945074735981 1104 34-35 0.4761698677381858 1104 36-37 0.43559393233547894 1104 38-39 0.4891587560684627 1104 40-41 0.5704324235877536 1104 42-43 0.6572878031970717 1104 44-45 0.5538171729329733 1104 46-47 0.6078986843263721 1104 48-49 0.7616904030701193 1104 50-51 0.7583765762825152 1104 52-53 0.7153661387154671 1104 54-55 0.9038877248772366 1104 56-57 0.9660978493441021 1104 58-59 0.9984231526565281 1104 60-61 0.7504039966702152 1104 62-63 0.9307527167835268 1104 64-65 1.1146176705000066 1104 66-67 1.1272368812686793 1104 68-69 1.3894218442087336 1104 70-71 1.5283201126949848 1104 72-73 1.5979546032458956 1104 74-75 1.675043757668142 1104 76-77 1.6949777018894032 1104 78-79 1.755867905325882 1104 80-81 1.8990463099643975 1104 82-83 1.9117094258860803 1104 84-85 2.152512581911555 1104 86-87 2.338191079493903 1104 88-89 2.0170288825003624 1104 90-91 2.297420831266326 1104 92-93 2.332133655069807 1104 94-95 2.352845442482419 1104 96-97 2.657858010809047 1104 98-99 2.665652007324166 1104 100-101 2.07226506727263 1105 1 0.08076350220314055 1105 2 0.1517680592293047 1105 3 0.1065076274337926 1105 4 0.14492092013684044 1105 5 -0.007128288413468908 1105 6 0.06636294401897658 1105 7 0.15110651829883182 1105 8 0.13393863130288253 1105 9 0.23809272570843376 1105 10-11 0.2542549790311668 1105 12-13 0.3281524050052056 1105 14-15 0.4192168830518952 1105 16-17 0.24998916084087597 1105 18-19 0.2621879826196434 1105 20-21 0.2344882735792737 1105 22-23 0.24298312480948425 1105 24-25 0.20114209063685706 1105 26-27 0.2886107762895307 1105 28-29 0.3235743332525729 1105 30-31 0.4679118071119319 1105 32-33 0.5694297759625044 1105 34-35 0.67394537796946 1105 36-37 0.5995974899561034 1105 38-39 0.6387267751371724 1105 40-41 0.7819242220111633 1105 42-43 0.7323546639965102 1105 44-45 0.7020809679266407 1105 46-47 0.8345010456237958 1105 48-49 0.876000590111623 1105 50-51 0.9434451377418327 1105 52-53 0.8624389389474203 1105 54-55 0.9281727039653305 1105 56-57 1.0150571831472277 1105 58-59 1.072015234952481 1105 60-61 1.0573500155941673 1105 62-63 1.1697683855003156 1105 64-65 1.3010482251859905 1105 66-67 1.1905854850180333 1105 68-69 1.289626811467702 1105 70-71 1.416450883227121 1105 72-73 1.4841796578290847 1105 74-75 1.349639406909482 1105 76-77 1.504881448092334 1105 78-79 1.579468046884724 1105 80-81 1.638555347661768 1105 82-83 1.939229166639798 1105 84-85 2.0714513612640353 1105 86-87 2.2058924844698637 1105 88-89 2.0771022728568624 1105 90-91 1.9091516989536537 1105 92-93 1.9130797370469388 1105 94-95 1.6315122849875507 1105 96-97 1.8221018365922035 1105 98-99 2.035031115494572 1105 100-101 2.084502136596056 1106 1 0.060629568019336944 1106 2 0.09661345947011313 1106 3 0.08813477094452082 1106 4 0.10599887387549956 1106 5 0.1197167411559974 1106 6 0.035424588218553765 1106 7 0.10369272854724443 1106 8 0.029054971558437614 1106 9 -0.0362701667204135 1106 10-11 0.11793437164858744 1106 12-13 0.11807092211246584 1106 14-15 0.18932781228347295 1106 16-17 0.033035094278154986 1106 18-19 0.14775914698127934 1106 20-21 0.10110750468248852 1106 22-23 0.22223499080271836 1106 24-25 0.21643344997680458 1106 26-27 0.035629399284374585 1106 28-29 0.19589088657934894 1106 30-31 0.3407290259622613 1106 32-33 0.19623268378845893 1106 34-35 0.1822753739646501 1106 36-37 0.29862013590146574 1106 38-39 0.33477264506782234 1106 40-41 0.36541690514030023 1106 42-43 0.3951592204027108 1106 44-45 0.33415207461388974 1106 46-47 0.14063176818665823 1106 48-49 0.23863082730240137 1106 50-51 0.4624029032416601 1106 52-53 0.3803307003232135 1106 54-55 0.29079477601814574 1106 56-57 0.563938753653197 1106 58-59 0.6781112232776323 1106 60-61 0.7502326073904015 1106 62-63 0.7059118223494458 1106 64-65 0.856424190907962 1106 66-67 0.8587442982476361 1106 68-69 0.7546889637203087 1106 70-71 0.7891466618507863 1106 72-73 1.0765042985306117 1106 74-75 0.8831146581170835 1106 76-77 0.8586166098981529 1106 78-79 1.1061689552887266 1106 80-81 1.3467007856177382 1106 82-83 1.5494835430889466 1106 84-85 1.6789722309678758 1106 86-87 1.64742711567132 1106 88-89 1.7351350189482915 1106 90-91 1.67452697776719 1106 92-93 1.546005591113893 1106 94-95 1.642141739361925 1106 96-97 1.7218204633456011 1106 98-99 2.0161113243665802 1106 100-101 1.948682453947752 1107 1 0.07196826755626873 1107 2 0.07721411961592395 1107 3 0.0418427007546569 1107 4 0.04757062946840307 1107 5 0.09349579381854056 1107 6 0.05555566020991165 1107 7 -0.0038217700764420215 1107 8 0.06337434228391459 1107 9 0.15553886655690263 1107 10-11 0.06521011149149558 1107 12-13 0.17821086556884325 1107 14-15 0.23556497107716012 1107 16-17 0.22039252615780214 1107 18-19 0.15047839458431866 1107 20-21 0.13359225543512565 1107 22-23 0.15333642776846546 1107 24-25 0.21039067752560214 1107 26-27 0.19231541592101564 1107 28-29 0.21332449920513596 1107 30-31 0.2603934014635101 1107 32-33 0.33461217292104095 1107 34-35 0.3195859800252592 1107 36-37 0.34670463496847503 1107 38-39 0.4601626923017008 1107 40-41 0.46048817912498663 1107 42-43 0.46062254664289526 1107 44-45 0.4906768834819175 1107 46-47 0.6212069877314903 1107 48-49 0.732215513773447 1107 50-51 0.5837225802618988 1107 52-53 0.5869159369157657 1107 54-55 0.643607927146391 1107 56-57 0.6803826513346252 1107 58-59 0.6405841957876746 1107 60-61 0.7689565118828625 1107 62-63 0.9064120953951083 1107 64-65 1.0698330500808844 1107 66-67 1.0724534068895792 1107 68-69 1.2695602544303064 1107 70-71 1.1520148956584748 1107 72-73 1.4100104264409161 1107 74-75 1.4044598261157297 1107 76-77 1.5073198193433157 1107 78-79 1.4813714725480303 1107 80-81 1.6726521885283425 1107 82-83 1.8439947712100277 1107 84-85 1.999634381801922 1107 86-87 2.239337835786422 1107 88-89 2.2095139408310374 1107 90-91 2.0771955204892656 1107 92-93 2.3096416272969726 1107 94-95 2.2968556056481404 1107 96-97 2.186865077595563 1107 98-99 2.203577949320735 1107 100-101 1.8404219915635682 1108 1 0.013534027132429571 1108 2 0.031566757137284185 1108 3 0.0563156354192742 1108 4 0.09799293661554742 1108 5 0.0060109963632157815 1108 6 0.01178330494006019 1108 7 0.010644196087724822 1108 8 0.07345263618994835 1108 9 0.07383023509990494 1108 10-11 0.07125804900759647 1108 12-13 0.07712703947441923 1108 14-15 0.19773154034501061 1108 16-17 0.12366517426486467 1108 18-19 0.05071313693242274 1108 20-21 0.02146904305573827 1108 22-23 0.10375678854135373 1108 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-0.5824026461824232 2316 60-61 -0.4369693205901335 2316 62-63 -0.5515515816623839 2316 64-65 -0.49472694498822634 2316 66-67 -0.6167046692413152 2316 68-69 -0.2848803398960875 2316 70-71 -0.33269308390914887 2316 72-73 -0.15724935957898012 2316 74-75 -0.18687817872270784 2316 76-77 -0.12179262678927216 2316 78-79 -0.3591668676936841 2316 80-81 -0.7632274553689093 2316 82-83 -0.05376148100501865 2316 84-85 -0.019286305338212628 2316 86-87 0.05426106670104858 2316 88-89 0.022961435835942012 2316 90-91 0.3582707242737264 2316 92-93 0.40210946736148756 2316 94-95 0.6125510538198604 2316 96-97 0.4366647601694851 2316 98-99 0.3677815784047702 2316 100-101 -0.07479125018874733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 24.0 4 52.0 5 128.0 6 263.0 7 694.0 8 1620.0 9 2953.0 10 5033.0 11 7588.0 12 10900.0 13 15070.0 14 20221.0 15 27466.0 16 34671.0 17 43715.0 18 43806.0 19 40016.0 20 41190.0 21 43544.0 22 48349.0 23 53476.0 24 60538.0 25 69306.0 26 78588.0 27 92530.0 28 108686.0 29 128003.0 30 148885.0 31 173823.0 32 204850.0 33 245067.0 34 299012.0 35 363216.0 36 414557.0 37 359313.0 38 116178.0 39 2496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46544795271669 19.074593935154283 15.12305242882481 23.336905683304217 2 16.000070187688532 20.460467540765585 38.884342488087 24.65511978345888 3 19.30524927475732 23.633594390448273 30.142658789578284 26.91849754521612 4 13.389268789660944 16.87087131416762 35.933836968220426 33.80602292795101 5 13.68696993154201 37.52941400673902 34.06207773374437 14.721538327974606 6 33.01384764026264 36.01617625083413 16.633412324998776 14.336563783904454 7 29.20960338601394 32.7904244435746 19.827371852307163 18.172600318104294 8 27.30907901825592 34.45787156088741 19.314447713751854 18.91860170710481 9 26.69211870195225 16.421705195615253 19.511280993010523 37.37489510942197 10-11 25.216133054472788 26.383266673402233 27.01728217448305 21.383318097641926 12-13 26.436929379999118 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28.696267957694232 22.595473004112026 38-39 22.16441979937781 26.246945544238443 28.711279091261403 22.877355565122343 40-41 22.748334553551437 26.023334402284732 28.8193028419989 22.40902820216493 42-43 22.42564112802958 26.242294272426246 28.466874068642312 22.865190530901867 44-45 22.32357252663949 26.38967622419465 28.90836606866828 22.378385180497585 46-47 22.946734740301384 26.821402297515423 27.85988519682791 22.371977765355286 48-49 22.4978477443211 26.885968865950318 27.688670997999292 22.927512391729287 50-51 22.793416124342603 27.078613142704683 27.720191031975595 22.40777970097712 52-53 23.293108486391027 26.551333188538806 27.78320085037543 22.372357474694738 54-55 22.386121021200704 26.999616435670998 28.035224999198388 22.579037543929914 56-57 22.588327573061378 27.08831222141103 27.655309924108394 22.668050281419195 58-59 22.459960179488746 26.567214203737997 27.693571425546477 23.279254191226784 60-61 21.850643438236766 26.930617205824614 28.201839535802463 23.016899820136157 62-63 22.202627234156346 26.96044326484633 28.239651476752915 22.59727802424441 64-65 21.814073100821986 27.071434221617164 27.90266231052611 23.21183036703474 66-67 21.66222171537056 27.07022982714829 28.00726175925692 23.260286698224235 68-69 22.69092544783796 26.47215900419853 28.022313512964107 22.8146020349994 70-71 22.24136851438049 26.765840863709688 27.4786717901358 23.51411883177402 72-73 22.195150503897963 26.92508503164714 27.553626504464233 23.32613795999066 74-75 22.20822340141701 27.278018194500998 27.37688885769824 23.136869546383757 76-77 22.232725539152902 26.728096450093986 27.818199582919167 23.220978427833945 78-79 22.31154074887003 27.083150575618742 27.64145144614037 22.963857229370863 80-81 22.576985937877648 27.086033738566794 27.333581178227202 23.00339914532836 82-83 22.411732721075627 27.101705412990363 27.4734613962289 23.013100469705115 84-85 22.177647092633684 27.341207718288814 27.434593144172638 23.046552044904868 86-87 22.549878785642072 27.314673925209497 26.894870518139196 23.240576771009227 88-89 22.196199204194766 27.460407872869602 26.648691374098032 23.694701548837603 90-91 22.05466461168947 27.32297374761593 27.049363315027097 23.572998325667502 92-93 22.253056688278523 26.84238077369201 27.251809646882343 23.652752891147124 94-95 21.825055617592355 26.51877355589748 27.551614441562165 24.104556384948 96-97 22.13057279948116 26.068943570669305 27.551927894746076 24.248555735103455 98-99 21.75098628666775 26.514186737749085 27.079656752274918 24.655170223308247 100-101 21.824695923841283 25.952808263454326 27.69112958821574 24.531366224488654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 429.0 2 373.5 3 487.0 4 424.5 5 441.0 6 815.0 7 1766.0 8 2761.0 9 3099.5 10 2731.0 11 2277.0 12 2236.0 13 2384.0 14 2627.0 15 2874.0 16 3138.5 17 3513.5 18 3993.5 19 4493.5 20 5153.0 21 6208.5 22 7623.5 23 9281.0 24 11454.0 25 14361.0 26 18135.5 27 22926.5 28 27485.5 29 32083.5 30 37759.0 31 46754.0 32 60914.0 33 64751.0 34 61330.0 35 64491.5 36 70324.0 37 76973.0 38 82566.0 39 86075.0 40 90775.0 41 98222.5 42 102744.5 43 105196.5 44 108269.5 45 115210.0 46 120389.0 47 122906.0 48 123435.5 49 121045.5 50 119745.0 51 116999.5 52 114099.0 53 116126.0 54 120574.0 55 115002.0 56 104742.5 57 95839.5 58 88398.0 59 77443.0 60 61049.5 61 50637.5 62 44288.0 63 33361.0 64 22405.5 65 16600.0 66 12651.5 67 9840.5 68 7591.5 69 5876.5 70 4601.0 71 3126.0 72 2288.5 73 1927.5 74 1428.5 75 1050.0 76 773.5 77 525.5 78 346.5 79 212.0 80 123.5 81 74.5 82 41.0 83 18.0 84 8.0 85 5.0 86 3.0 87 2.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0248046332634972 2 0.012432566184508961 3 0.0013309803214559472 4 0.0 5 3.327450803639868E-4 6 0.0 7 0.0 8 9.07486582810873E-5 9 2.419964220828995E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015124776380181218 26-27 0.00905974105172855 28-29 1.3612298742163097E-4 30-31 0.0 32-33 3.327450803639868E-4 34-35 0.010133600174721417 36-37 0.001315855545075766 38-39 0.012614063501071136 40-41 0.012523314842790048 42-43 0.005323921285823789 44-45 8.469874772901483E-4 46-47 6.0499105520724873E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.5124776380181218E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.56238819009061E-5 66-67 7.108644898685172E-4 68-69 0.0010436095702325043 70-71 3.0249552760362437E-5 72-73 1.814973165621746E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.07486582810873E-5 82-83 0.0011041086757532291 84-85 0.005414669944104876 86-87 0.0036299463312434925 88-89 0.0 90-91 0.001179732557654135 92-93 2.8737075122344316E-4 94-95 1.5124776380181218E-5 96-97 0.0 98-99 0.0 100-101 3.0249552760362437E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3305834.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.10453147382213 #Duplication Level Percentage of deduplicated Percentage of total 1 72.91852298007817 35.07711383719792 2 14.344236224274907 13.800455258371436 3 5.315253763548095 7.670633758798526 4 2.452324829180874 4.718717477174673 5 1.3519248123630572 3.2516854843279877 6 0.842787516206159 2.4325139159450133 7 0.5350170259512882 1.8015720354733116 8 0.3812696568501442 1.4672638566368899 9 0.2801128758058832 1.212722878538424 >10 1.4174390202225062 12.093221378548973 >50 0.09571103204246269 3.1362168492353124 >100 0.054811889628616516 5.074639460363537 >500 0.005798395203136927 1.9081781219681435 >1k 0.0042227443315123745 4.443400952320601 >5k 5.042082783895372E-4 1.581224725141777 >10k+ 6.302603479869216E-5 0.33044000995743816 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10899 0.3296898755351902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8811 0.26652880937155343 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6805 0.2058482065342664 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6724 0.20339799276067705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6391 0.19332489169147635 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6307 0.19078392925960588 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6163 0.1864279936621137 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5623 0.17009323517151798 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5330 0.16123011621273178 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4777 0.14450211353625136 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4418 0.13364252409528124 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4278 0.1294075867088305 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4272 0.12922608939226835 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4085 0.12356942302608055 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3905 0.11812450352921532 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3497 0.10578268600298746 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3454 0.10448195523429185 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3449 0.10433070747049006 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3398 0.10278798027971156 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 3316 0.10030751695336185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0249552760362437E-5 0.0 10-11 1.5124776380181218E-5 0.0 0.0 3.0249552760362437E-5 3.0249552760362437E-5 12-13 3.0249552760362437E-5 0.0 0.0 3.0249552760362437E-5 1.814973165621746E-4 14-15 3.0249552760362437E-5 0.0 0.0 3.0249552760362437E-5 2.8737075122344316E-4 16-17 3.0249552760362437E-5 0.0 0.0 6.0499105520724873E-5 3.629946331243493E-4 18-19 7.56238819009061E-5 0.0 0.0 9.074865828108732E-5 3.629946331243493E-4 20-21 9.074865828108732E-5 0.0 0.0 1.2099821104144976E-4 4.083689622648929E-4 22-23 9.074865828108732E-5 0.0 0.0 2.2687164570271828E-4 4.83992844165799E-4 24-25 1.2099821104144975E-4 0.0 0.0 5.444919496865239E-4 5.142423969261615E-4 26-27 1.2099821104144975E-4 0.0 0.0 0.00124023166317486 5.444919496865239E-4 28-29 1.512477638018122E-4 0.0 0.0 0.0039778161879876605 5.444919496865239E-4 30-31 1.512477638018122E-4 0.0 0.0 0.012931683805054942 5.596167260667051E-4 32-33 1.512477638018122E-4 0.0 0.0 0.03133853665973549 6.049910552072487E-4 34-35 1.663725401819934E-4 0.0 0.0 0.058275763392838235 6.95739713488336E-4 36-37 2.1174686932253707E-4 0.0 0.0 0.10145699995825562 6.95739713488336E-4 38-39 2.1174686932253707E-4 0.0 0.0 0.17337531164601733 7.108644898685173E-4 40-41 2.1174686932253707E-4 0.0 0.0 0.2530677583931922 7.259892662486986E-4 42-43 2.2687164570271828E-4 0.0 0.0 0.3358305347455438 7.259892662486986E-4 44-45 2.7224597484326195E-4 0.0 0.0 0.44141659865558885 7.713635953892422E-4 46-47 3.176203039838056E-4 0.0 0.0 0.5663018772267452 8.621122536703295E-4 48-49 3.327450803639868E-4 0.0 0.0 0.6982050520383056 9.67985688331598E-4 50-51 3.327450803639868E-4 0.0 0.0 0.8490444468778529 9.67985688331598E-4 52-53 3.327450803639868E-4 0.0 0.0 0.9920643323288465 0.0010587343466126854 54-55 3.327450803639868E-4 0.0 0.0 1.144416204806412 0.0011192334521334102 56-57 3.327450803639868E-4 0.0 0.0 1.322374323695624 0.0011192334521334102 58-59 3.327450803639868E-4 0.0 0.0 1.512235641596039 0.0011192334521334102 60-61 3.327450803639868E-4 0.0 0.0 1.7216986696851686 0.0011192334521334102 62-63 3.327450803639868E-4 0.0 0.0 1.9288022326589902 0.0012099821104144975 64-65 3.327450803639868E-4 0.0 0.0 2.1433018112827202 0.0012099821104144975 66-67 3.629946331243493E-4 0.0 0.0 2.402358981122464 0.0012099821104144975 68-69 3.781194095045305E-4 0.0 0.0 2.6858426648162004 0.00124023166317486 70-71 4.3861851502525535E-4 0.0 0.0 2.9794599486846587 0.0012856059923154035 72-73 4.991176205459803E-4 0.0 0.0 3.2914084615258963 0.001391479426976672 74-75 5.142423969261615E-4 0.0 0.0 3.5976398088954253 0.0014973528616379407 76-77 5.142423969261615E-4 0.0 0.0 3.9308991316563384 0.001512477638018122 78-79 5.142423969261615E-4 0.0 0.0 4.3152499490295035 0.0015881015199190282 80-81 6.352406079676112E-4 0.0 0.0 4.717009384016258 0.0016637254018199341 82-83 6.352406079676112E-4 6.0499105520724873E-5 0.0 5.132774361931059 0.0018300979420019276 84-85 6.503653843477924E-4 6.0499105520724873E-5 0.0 5.556510096998216 0.001875472271142471 86-87 6.654901607279736E-4 6.0499105520724873E-5 0.0 5.992875020342824 0.0019057218239028335 88-89 6.654901607279736E-4 6.0499105520724873E-5 0.0 6.456525040277279 0.001935971376663196 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6250 0.0 53.518723 1 GTATCAA 11210 0.0 42.384544 1 TATCAAC 14395 0.0 32.86766 2 ATCAACG 14410 0.0 32.79701 3 TCAACGC 14770 0.0 31.99714 4 CAACGCA 14925 0.0 31.56937 5 AACGCAG 15465 0.0 30.52847 6 TAAGGTG 2505 0.0 28.820694 5 AGGTAAG 2305 0.0 27.203459 2 ACGCAGA 17780 0.0 26.259737 7 CGCAGAG 17910 0.0 25.989569 8 GGTAAGG 2685 0.0 25.119976 3 GTAAGGT 2745 0.0 25.08963 4 TGGTATC 3030 0.0 24.456995 2 GTGGTAT 3285 0.0 23.720377 1 AAGGTAA 2610 0.0 23.119387 1 GCAGAGT 20195 0.0 22.766699 9 GTACATG 13000 0.0 22.513885 1 GAGTACT 11815 0.0 22.210949 12-13 TACATGG 12845 0.0 22.152046 2 >>END_MODULE