FastQCFastQC Report
Thu 26 May 2016
SRR938355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361476
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61680.45303773257846636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.36350255164248213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.2645658094597334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13820.10150748158616091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.049.0292851
GTATCAA53700.040.9957961
ATCAACG68800.031.8587783
TCAACGC70050.031.426034
TATCAAC70900.030.9822082
CAACGCA71250.030.896755
AACGCAG74650.029.5973196
TGGTATC13750.027.6632982
GTACATG59750.026.2608411
TACATGG58900.025.83162
CGCAGAG84250.025.775528
GTGGTAT15500.025.767971
ACGCAGA84650.025.653727
GTACCGT950.001224440425.0078056
ACATGGG60650.023.8319423
GCAGAGT89650.023.4270259
CATGGGG27550.022.608224
GAGTACT54600.021.92992212-13
AGTACTT55650.021.30269612-13
GAGTACA45500.021.109241
Produced by FastQC (version 0.11.5)