##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938355_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1361476 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.016569517200452 31.0 31.0 34.0 28.0 34.0 2 31.04158648408051 31.0 31.0 34.0 27.0 34.0 3 31.07407328516992 31.0 31.0 34.0 27.0 34.0 4 34.73032502960023 37.0 35.0 37.0 32.0 37.0 5 34.71509670387139 37.0 35.0 37.0 32.0 37.0 6 34.736999403588456 37.0 35.0 37.0 32.0 37.0 7 34.64748405407073 37.0 35.0 37.0 30.0 37.0 8 34.61788015359801 37.0 35.0 37.0 30.0 37.0 9 36.09180624557465 39.0 35.0 39.0 30.0 39.0 10-11 35.917324653537776 38.0 35.0 39.0 30.0 39.0 12-13 35.76199396831086 38.0 35.0 39.0 30.0 39.0 14-15 36.68522728274314 39.0 36.0 41.0 30.0 41.0 16-17 36.51502266657657 39.0 36.0 40.5 30.0 41.0 18-19 36.67914087358132 39.0 36.0 41.0 30.0 41.0 20-21 36.66722476194953 39.0 36.0 41.0 30.0 41.0 22-23 36.557635977424496 39.0 36.0 41.0 30.0 41.0 24-25 36.42195565694878 39.0 36.0 41.0 29.0 41.0 26-27 36.19884889634485 39.0 35.0 41.0 28.5 41.0 28-29 35.97717917906742 39.0 35.0 40.0 27.0 41.0 30-31 35.7032151135973 38.0 35.0 40.0 27.0 41.0 32-33 35.400955654010794 38.0 34.0 40.0 26.5 41.0 34-35 35.12646752495086 38.0 34.0 40.0 25.5 41.0 36-37 34.83080715341291 38.0 34.0 40.0 25.0 41.0 38-39 34.67258548810262 38.0 33.5 40.0 24.0 41.0 40-41 34.55140781034701 38.0 33.0 40.0 24.0 41.0 42-43 34.24480637190813 38.0 33.0 40.0 23.5 41.0 44-45 33.87241236716622 38.0 33.0 40.0 21.5 41.0 46-47 33.44128725001396 38.0 32.5 40.0 18.5 41.0 48-49 33.05258116926042 37.5 31.5 40.0 16.0 41.0 50-51 32.36220616448619 36.5 30.5 39.5 14.5 40.5 52-53 32.393468926371085 37.0 31.0 39.0 13.5 40.0 54-55 32.49422538480296 37.0 31.0 40.0 10.5 41.0 56-57 32.17087264116297 37.0 31.0 40.0 8.0 41.0 58-59 31.80800175691676 36.0 30.5 39.0 7.0 41.0 60-61 31.322867975638204 36.0 30.0 39.0 2.0 40.5 62-63 30.747354341905403 35.0 29.0 39.0 2.0 40.0 64-65 30.220615714122026 35.0 28.0 38.5 2.0 40.0 66-67 29.680750156447857 34.5 28.0 38.0 2.0 40.0 68-69 29.09553822469144 34.0 26.5 37.5 2.0 40.0 70-71 28.472479132941015 34.0 26.0 37.0 2.0 39.0 72-73 27.829021591273 33.5 25.5 36.0 2.0 39.0 74-75 27.13976338914531 33.0 24.0 36.0 2.0 38.5 76-77 26.396891682262485 32.0 21.5 35.0 2.0 37.5 78-79 25.610232203872854 31.5 19.5 35.0 2.0 37.0 80-81 24.909428076587467 31.0 17.0 35.0 2.0 36.5 82-83 24.165073053068873 31.0 9.0 34.0 2.0 36.0 84-85 23.443485232203873 30.0 4.5 34.0 2.0 35.5 86-87 22.674780899553134 29.5 2.0 34.0 2.0 35.0 88-89 21.849454195299806 29.0 2.0 34.0 2.0 35.0 90-91 20.93787624607411 28.5 2.0 34.0 2.0 35.0 92-93 19.992565788893817 26.5 2.0 33.5 2.0 35.0 94-95 18.999211517500125 25.5 2.0 33.0 2.0 35.0 96-97 17.762349832093992 23.0 2.0 32.5 2.0 34.0 98-99 16.28865327042122 10.0 2.0 32.0 2.0 34.0 100-101 12.899369140550402 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46910235437205117 1101 2 0.4472999187428286 1101 3 0.2529212680030426 1101 4 0.408689475077459 1101 5 0.39579974008915286 1101 6 0.5324063459130812 1101 7 0.5483471432727853 1101 8 0.4769100792184844 1101 9 0.6516316247219507 1101 10-11 0.7524437931085686 1101 12-13 0.734952902094463 1101 14-15 0.9656205105275646 1101 16-17 0.6294700504670487 1101 18-19 0.791363599572513 1101 20-21 0.8725368569377991 1101 22-23 0.9200794420770038 1101 24-25 0.9831282138571495 1101 26-27 1.0260631931348811 1101 28-29 1.1197891561594489 1101 30-31 0.9042414962323804 1101 32-33 1.0643346290322313 1101 34-35 1.045871836738037 1101 36-37 0.8753192590472025 1101 38-39 1.0177092989369214 1101 40-41 0.8630178959213239 1101 42-43 1.1805839741144268 1101 44-45 1.102474030575742 1101 46-47 1.1614888703466661 1101 48-49 0.9737682008277204 1101 50-51 0.9915017792662297 1101 52-53 1.1431501138668239 1101 54-55 1.190365715701482 1101 56-57 1.1443366692467691 1101 58-59 1.160542060149595 1101 60-61 1.360263596062424 1101 62-63 1.2488286804931867 1101 64-65 1.370021052773037 1101 66-67 1.0701671652496252 1101 68-69 0.8651919408730606 1101 70-71 1.1321151251177533 1101 72-73 1.2407125653463993 1101 74-75 1.07019811425576 1101 76-77 1.1269647649661465 1101 78-79 1.5424013936844858 1101 80-81 1.2375395650025744 1101 82-83 1.2124477600047747 1101 84-85 1.112524849786471 1101 86-87 1.352988816448871 1101 88-89 1.1538964660756754 1101 90-91 1.151372959829196 1101 92-93 1.1322782752151639 1101 94-95 1.1994766304548037 1101 96-97 1.0529737212776524 1101 98-99 0.972702233176026 1101 100-101 0.7932068114277087 1102 1 0.5800948237437495 1102 2 0.5660665441971027 1102 3 0.45606090308602987 1102 4 0.5305095642460955 1102 5 0.493510578948694 1102 6 0.584109167088755 1102 7 0.5277784122398543 1102 8 0.607347305249732 1102 9 0.8168745905156669 1102 10-11 0.7955856761403055 1102 12-13 0.5969382154959888 1102 14-15 0.883139524427385 1102 16-17 0.995426739987856 1102 18-19 0.8934954043007011 1102 20-21 0.8587588044107264 1102 22-23 0.9041564717873882 1102 24-25 0.8614392550320744 1102 26-27 0.8636863619933024 1102 28-29 0.7022045770878478 1102 30-31 0.9196212122415304 1102 32-33 0.8813235016859267 1102 34-35 0.8155413882722655 1102 36-37 0.9040087049278327 1102 38-39 0.7763358022526461 1102 40-41 1.0206834011478136 1102 42-43 0.9944631843351033 1102 44-45 1.1908652485893043 1102 46-47 1.193316078019656 1102 48-49 1.3329997770144502 1102 50-51 1.3016812405078468 1102 52-53 1.2443452732838622 1102 54-55 0.9623487811542546 1102 56-57 1.1243392918536443 1102 58-59 1.002726691673331 1102 60-61 1.085339464332634 1102 62-63 1.201818452326389 1102 64-65 1.088849496817847 1102 66-67 0.867552051540887 1102 68-69 0.6888871939546277 1102 70-71 0.7877012777534702 1102 72-73 0.8553766843378199 1102 74-75 0.6164028274549658 1102 76-77 0.632462872942618 1102 78-79 0.9945388182845392 1102 80-81 1.3047906983433997 1102 82-83 1.0947301772989952 1102 84-85 1.2400022766344705 1102 86-87 1.2658433366991453 1102 88-89 1.307272135777076 1102 90-91 1.2947171582481367 1102 92-93 1.2230766716783918 1102 94-95 1.1033487221711127 1102 96-97 1.3545266792035449 1102 98-99 1.3748415310667035 1102 100-101 1.231744146109813 1103 1 0.3276141065559983 1103 2 0.32416136272072293 1103 3 0.2962157716319105 1103 4 0.29741771768809855 1103 5 0.3762764416695177 1103 6 0.4427519927637462 1103 7 0.3736278000149369 1103 8 0.5092899218381959 1103 9 0.5323863863054967 1103 10-11 0.6047988861195819 1103 12-13 0.5000865177460483 1103 14-15 0.5017127944520254 1103 16-17 0.43209997994319593 1103 18-19 0.5878013512621862 1103 20-21 0.5283047813016566 1103 22-23 0.6415442655166572 1103 24-25 0.5441044205647287 1103 26-27 0.5975672362873112 1103 28-29 0.5373776154157142 1103 30-31 0.7242068969241302 1103 32-33 0.7533139494387768 1103 34-35 0.6092659954483395 1103 36-37 0.7434264094515157 1103 38-39 0.6409872431315051 1103 40-41 0.8489370042190743 1103 42-43 0.7727819375547327 1103 44-45 0.8976919118993933 1103 46-47 0.5666881984180705 1103 48-49 0.37609619224116386 1103 50-51 0.6184444086465106 1103 52-53 0.7488585818624642 1103 54-55 0.7232290890265887 1103 56-57 0.8120218346441135 1103 58-59 0.7417414908966578 1103 60-61 0.8611763348877943 1103 62-63 0.7036173656359033 1103 64-65 1.0225612604405185 1103 66-67 0.9050629196112538 1103 68-69 1.0957760937212981 1103 70-71 1.1905599590398808 1103 72-73 1.0721716699908015 1103 74-75 1.0211367607757147 1103 76-77 1.2253531153384642 1103 78-79 0.9550891796988665 1103 80-81 1.1796773557328706 1103 82-83 1.2861393318854937 1103 84-85 1.5564857303615476 1103 86-87 1.5885661700196572 1103 88-89 1.283814003197108 1103 90-91 1.1294385743489634 1103 92-93 1.3194586823122023 1103 94-95 1.4087758810419047 1103 96-97 1.8025633171295574 1103 98-99 1.7089965254840713 1103 100-101 1.879367505992704 1104 1 0.4982568073496374 1104 2 0.38378061241074235 1104 3 0.37915743259135226 1104 4 0.272968409045788 1104 5 0.4257659247067451 1104 6 0.42192845123975786 1104 7 0.46089016679037087 1104 8 0.6004824411196665 1104 9 0.7051676182605959 1104 10-11 0.7794685384487963 1104 12-13 0.714742002725977 1104 14-15 0.772294058636156 1104 16-17 0.9277602500404711 1104 18-19 0.7604359858430527 1104 20-21 0.7333866743997177 1104 22-23 0.8433786351031856 1104 24-25 0.8675530622449372 1104 26-27 0.8232074527590427 1104 28-29 0.874675792236765 1104 30-31 0.8752799673222071 1104 32-33 0.848059496683419 1104 34-35 0.700818293781829 1104 36-37 0.90069182008083 1104 38-39 1.0144429349144488 1104 40-41 1.170548142933761 1104 42-43 0.947058238914174 1104 44-45 1.0196939396432256 1104 46-47 0.9465259117676013 1104 48-49 0.7743833529969351 1104 50-51 0.8403827616529895 1104 52-53 1.010579657417189 1104 54-55 1.110181251281908 1104 56-57 1.0675427365201102 1104 58-59 1.3222861169278701 1104 60-61 1.3186479889985634 1104 62-63 1.3183852046339375 1104 64-65 1.5547018954421539 1104 66-67 1.7213372424656725 1104 68-69 1.760932583620626 1104 70-71 1.4956777740899447 1104 72-73 1.696901975584776 1104 74-75 1.6645095523030555 1104 76-77 1.5701557348433006 1104 78-79 1.6151735123562858 1104 80-81 1.7691358854619246 1104 82-83 1.748766513500616 1104 84-85 1.6232876918567989 1104 86-87 1.7634848140722816 1104 88-89 1.7719401754165673 1104 90-91 1.926943957046447 1104 92-93 2.178123706193027 1104 94-95 2.0258165388724407 1104 96-97 1.932359625113854 1104 98-99 2.027518488232669 1104 100-101 1.972325348616506 1105 1 0.371081902576492 1105 2 0.23641453082296593 1105 3 0.21302512096612602 1105 4 0.2245308525530305 1105 5 0.14551398560939788 1105 6 0.2407762435293037 1105 7 0.3051083920723343 1105 8 0.23422326669555105 1105 9 0.3983808307073957 1105 10-11 0.594777092595713 1105 12-13 0.6422340113266998 1105 14-15 0.6429522245382415 1105 16-17 0.6427772749642813 1105 18-19 0.4918234161110533 1105 20-21 0.5198860480611742 1105 22-23 0.7165913371212937 1105 24-25 0.7310309319707713 1105 26-27 0.563203050311536 1105 28-29 0.5836904947100052 1105 30-31 0.5734935200548037 1105 32-33 0.7568006944468806 1105 34-35 0.7846729555909135 1105 36-37 0.56872866877913 1105 38-39 0.682310969955374 1105 40-41 0.9266743506824966 1105 42-43 0.802436774962139 1105 44-45 0.983033196076228 1105 46-47 0.8265568246055253 1105 48-49 0.8994016965282157 1105 50-51 0.8353635461393623 1105 52-53 0.7815715692491381 1105 54-55 0.9004535437235219 1105 56-57 0.8158080764975324 1105 58-59 0.8961940653660676 1105 60-61 0.8250206381022309 1105 62-63 0.6775318741930327 1105 64-65 0.6997556286963693 1105 66-67 0.7591951008344715 1105 68-69 0.7426797066105486 1105 70-71 0.7421108877262625 1105 72-73 0.8505795611719726 1105 74-75 1.1095238868422754 1105 76-77 1.1998742759530145 1105 78-79 1.2088483437553066 1105 80-81 1.3445371036850773 1105 82-83 1.307963215705879 1105 84-85 1.3425681256361166 1105 86-87 1.3174222397676871 1105 88-89 1.2982817521733452 1105 90-91 0.9857698009020552 1105 92-93 1.1047987630313898 1105 94-95 1.1164617632802916 1105 96-97 1.1366573815670549 1105 98-99 1.133059989275857 1105 100-101 1.086905691156696 1106 1 0.16171878282019847 1106 2 0.011788628737729567 1106 3 -0.030422446081626475 1106 4 -0.1313172093952275 1106 5 0.05301213026147167 1106 6 -0.22897933719327312 1106 7 0.21149994004345984 1106 8 0.1053930906715621 1106 9 0.052371534660515806 1106 10-11 -0.010787046901057806 1106 12-13 -0.07360391563474167 1106 14-15 0.13768160822419162 1106 16-17 -0.008372088610393291 1106 18-19 -0.034062853044794394 1106 20-21 0.08622842336143322 1106 22-23 0.15142829131442426 1106 24-25 0.026512346489091954 1106 26-27 -0.07406333850696711 1106 28-29 0.028495018003290795 1106 30-31 -0.37953139794225166 1106 32-33 -0.03752334266324908 1106 34-35 -0.4225422980931981 1106 36-37 -0.18939717326847472 1106 38-39 -0.16319342813167736 1106 40-41 0.059129530078642745 1106 42-43 -0.643707618977821 1106 44-45 -0.42920765691303586 1106 46-47 -0.4012193339488377 1106 48-49 0.03413539657597653 1106 50-51 -0.29022882955761986 1106 52-53 -0.69992467429946 1106 54-55 -0.5241582349719565 1106 56-57 -1.221208164342766 1106 58-59 -1.0833283418943083 1106 60-61 -0.6401863647716404 1106 62-63 -0.7519540608679414 1106 64-65 -0.6373950752779116 1106 66-67 -1.0124553415661666 1106 68-69 -0.9566074060343297 1106 70-71 -1.0601970963264762 1106 72-73 -0.6906668200400041 1106 74-75 -0.04625313498444328 1106 76-77 -0.1440553906239046 1106 78-79 -0.24538102961580677 1106 80-81 -0.09599628437859664 1106 82-83 -0.5684478669762534 1106 84-85 -0.48720165253145 1106 86-87 -0.5631978246497837 1106 88-89 -0.41161482970184693 1106 90-91 -0.24508784855329324 1106 92-93 -0.22838972180395345 1106 94-95 0.15166735381518848 1106 96-97 -3.545746493784918E-4 1106 98-99 0.18593806633666787 1106 100-101 -0.0866654793549344 1107 1 0.1254398013467899 1107 2 0.11036847882161638 1107 3 0.23060141633622067 1107 4 0.09224352920586654 1107 5 0.022799569528004326 1107 6 0.24601822577201204 1107 7 0.3121392127296829 1107 8 0.21299971735376033 1107 9 0.2674734540177539 1107 10-11 0.5140081139175763 1107 12-13 0.37336877418460546 1107 14-15 0.4430951601894648 1107 16-17 0.6015684477424728 1107 18-19 0.4723498831551822 1107 20-21 0.6039985109407553 1107 22-23 0.5650074487673962 1107 24-25 0.33767806998056216 1107 26-27 0.5962317735709703 1107 28-29 0.5809210219333281 1107 30-31 0.4682585963627801 1107 32-33 0.7248670549069018 1107 34-35 0.726752757548148 1107 36-37 0.959477429751395 1107 38-39 0.7248566362573499 1107 40-41 0.7648134646327165 1107 42-43 0.8578148863964827 1107 44-45 0.9186836667303027 1107 46-47 0.7851591811530838 1107 48-49 0.7420267715253104 1107 50-51 0.6198286844617442 1107 52-53 0.6583930534606779 1107 54-55 0.8950214847947038 1107 56-57 0.6064544884849639 1107 58-59 0.5073924560165182 1107 60-61 0.794726851139373 1107 62-63 0.586760929204349 1107 64-65 0.779798332927971 1107 66-67 0.8920377808653264 1107 68-69 0.9491924800663654 1107 70-71 0.937520813056878 1107 72-73 0.9556468442854005 1107 74-75 1.049280165171016 1107 76-77 0.9677319049858397 1107 78-79 1.244219245384322 1107 80-81 1.2350738560459114 1107 82-83 1.0483792164319006 1107 84-85 0.8847585955843371 1107 86-87 1.1933185496582297 1107 88-89 1.33989972324337 1107 90-91 0.9648588445188544 1107 92-93 0.8543717207153776 1107 94-95 1.022176668418414 1107 96-97 0.9357143087782731 1107 98-99 0.8401074479455239 1107 100-101 0.9093949898896874 1108 1 0.3181657669699547 1108 2 0.3010161489085377 1108 3 0.4019562297471069 1108 4 0.35549429712953184 1108 5 0.24724895192131413 1108 6 0.36158583573214287 1108 7 0.5172565253236314 1108 8 0.522816069226181 1108 9 0.487478682434201 1108 10-11 0.6744420724823783 1108 12-13 0.49074672333825475 1108 14-15 0.6221259424912802 1108 16-17 0.5249982889293179 1108 18-19 0.6507957363673853 1108 20-21 0.4437508147117555 1108 22-23 0.26779158052348606 1108 24-25 0.6657612018020203 1108 26-27 0.8775565235873088 1108 28-29 0.7195916970526 1108 30-31 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-2.1363290360070515 2316 76-77 -2.2617571355810426 2316 78-79 -2.7209227317337046 2316 80-81 -2.6005767541556004 2316 82-83 -2.716055317763111 2316 84-85 -2.544068598512318 2316 86-87 -2.7755286045182643 2316 88-89 -2.8926605641073344 2316 90-91 -3.1130203621028656 2316 92-93 -3.0801157146702636 2316 94-95 -3.7368395648493173 2316 96-97 -3.9103406675162233 2316 98-99 -3.6458390739291424 2316 100-101 -3.264530585532894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5833.0 3 2020.0 4 2192.0 5 2430.0 6 2923.0 7 3518.0 8 4545.0 9 5677.0 10 7041.0 11 8709.0 12 10587.0 13 12246.0 14 13902.0 15 15453.0 16 17760.0 17 20610.0 18 19680.0 19 18076.0 20 18994.0 21 20589.0 22 22254.0 23 24721.0 24 27385.0 25 29960.0 26 33796.0 27 37837.0 28 42945.0 29 48799.0 30 55164.0 31 64510.0 32 74650.0 33 88764.0 34 106404.0 35 129712.0 36 149486.0 37 144198.0 38 65136.0 39 2970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.728013762677556 18.776035487963167 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22.26322918438475 44-45 21.913429596125983 26.88897825915295 29.09846827937145 22.09912386534962 46-47 22.161658664441322 27.13084960713677 28.490848373011442 22.216643355410465 48-49 22.08477736136229 27.068124822786725 28.548672865397247 22.29842495045374 50-51 22.218888109841085 27.312073286157073 28.2826917574743 22.186346846527545 52-53 22.376347154626522 27.246983949288257 28.233767941401915 22.142900954683302 54-55 22.107317281003674 27.278884405082277 28.33047519739135 22.283323116522695 56-57 22.035939008806796 27.49022334518616 28.30500885748214 22.168828788524905 58-59 22.088855862583472 26.993519342611638 28.420086768740475 22.497538026064415 60-61 22.00527357496661 27.282081159275485 28.433186934934753 22.279458330823157 62-63 21.969137126536754 27.31728655884911 28.58679714863308 22.126779165981052 64-65 21.802231091676177 27.16091325421833 28.48401019134294 22.552845462762555 66-67 21.765519318459372 27.319252714721475 28.342996082867643 22.57223188395151 68-69 21.996174284551543 27.077294437580164 28.515647381867815 22.410883896000485 70-71 21.902310017152193 27.10806836337079 28.30802383893911 22.681597780537903 72-73 21.964171899939938 27.211098519009685 28.15057132833609 22.674158252714292 74-75 21.92477792394476 27.521708000983047 28.01892472612671 22.534589348945484 76-77 22.102771827910615 27.260685241027478 28.011966426708756 22.624576504353147 78-79 22.127234952279274 27.379838777503185 28.00084911935085 22.49207715086669 80-81 22.15482895699465 27.510408311370842 27.740757390121434 22.594005341513075 82-83 22.23513787675454 27.482027652477566 27.765449754920862 22.51738471584703 84-85 22.189763864837918 27.425724574473747 27.792258351222358 22.592253209465973 86-87 22.304611242624198 27.42030092724378 27.609936879840298 22.665150950291725 88-89 22.292373279880554 27.40537506098672 27.591641733139742 22.71060992599298 90-91 22.204873905494697 27.41228344096229 27.62091633890594 22.761926314637073 92-93 22.313944235987176 27.174357870390438 27.664748794503097 22.84694909911929 94-95 22.23604981165574 27.087476098723844 27.668675770095074 23.007798319525342 96-97 22.48481120928346 26.92641188884774 27.57019028975539 23.01858661211341 98-99 22.256242483195788 26.999626042434265 27.314183865163688 23.429947609206263 100-101 22.852883679236648 26.54636589267974 27.591224350358544 23.009526077725063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1030.0 1 704.0 2 744.5 3 994.0 4 671.5 5 570.0 6 920.5 7 1802.5 8 2689.0 9 2870.0 10 2377.0 11 1772.5 12 1588.5 13 1640.0 14 1757.0 15 1856.0 16 1948.0 17 2035.0 18 2180.5 19 2385.0 20 2628.5 21 3058.5 22 3573.0 23 4296.5 24 5144.0 25 6182.0 26 7659.5 27 9573.0 28 11389.0 29 13326.5 30 15580.5 31 18259.0 32 21819.5 33 24303.5 34 25940.5 35 28350.0 36 31365.0 37 34228.5 38 36396.5 39 37904.0 40 39604.0 41 42176.0 42 44817.0 43 46978.5 44 48515.0 45 50128.0 46 50929.5 47 51649.0 48 52282.0 49 51410.0 50 50033.0 51 48070.5 52 45949.5 53 44738.0 54 43902.0 55 41214.5 56 37163.5 57 33526.5 58 30647.0 59 27209.0 60 22651.0 61 18653.0 62 15347.5 63 11920.0 64 9068.5 65 7104.0 66 5411.5 67 4161.5 68 3256.0 69 2470.5 70 1875.5 71 1404.5 72 1083.0 73 883.5 74 653.5 75 488.5 76 368.0 77 238.5 78 161.5 79 114.5 80 71.0 81 47.5 82 31.0 83 18.0 84 14.5 85 11.0 86 7.0 87 6.5 88 3.5 89 2.0 90 1.5 91 1.5 92 2.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008299815788159321 2 0.11149664041084824 3 0.11898850952936373 4 0.12317514227206355 5 0.1639397242404567 6 0.09878984278826804 7 0.025193246153439357 8 0.027617086162370838 9 0.021961459474864047 10-11 0.05240635898098828 12-13 0.0544996753523382 14-15 0.027617086162370838 16-17 0.031546644964729455 18-19 0.03621070073949156 20-21 0.05997167779674412 22-23 0.030701973446465452 24-25 0.048403350481389315 26-27 0.0578783614253942 28-29 0.05479347414129959 30-31 0.07025463541039284 32-33 0.051451512916863756 34-35 0.06415831053944396 36-37 0.05490364868716011 38-39 0.033272712849877636 40-41 0.03801021832188008 42-43 0.03382358557918024 44-45 0.007234795178174275 46-47 0.013808543081185419 48-49 0.008960863063322453 50-51 0.009364836398144366 52-53 0.009878984278826802 54-55 0.011568327315354806 56-57 0.014506315204968725 58-59 0.019941592800754478 60-61 0.02570739403412179 62-63 0.012045750347417068 64-65 0.009144487306423322 66-67 0.010907280040191675 68-69 0.011898850952936373 70-71 0.03125284617576806 72-73 0.028902455864076932 74-75 0.02934315404751902 76-77 0.024568923726896397 78-79 0.004810955169242792 80-81 0.00470078062338227 82-83 0.00580252608198749 84-85 0.005398552747165576 86-87 0.013478019443603854 88-89 0.03848764135394234 90-91 0.03474170679468459 92-93 0.030775423143705804 94-95 0.049211297151033145 96-97 0.04436361713317018 98-99 0.026368441309284923 100-101 0.07730580634546624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1361476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.83536747005992 #Duplication Level Percentage of deduplicated Percentage of total 1 84.17955079184392 54.578121090537756 2 9.496808444135876 12.314581305366351 3 2.7016310682377385 5.25483729233173 4 1.0929320904503852 2.83442614816686 5 0.5936756273868736 1.9245588729823164 6 0.3700211457737637 1.439427417476075 7 0.2603071832355689 1.1813978316122031 8 0.1929418967946843 1.0007567023242987 9 0.1436267383843056 0.8380883125505352 >10 0.8766784568193577 10.448005650001319 >50 0.06230870461881331 2.782016230417404 >100 0.02690670022083195 3.1726957894716703 >500 0.0019299819854511591 0.8620796121008896 >1k 5.676417604268115E-4 0.9150027030418509 >5k 1.1352835208536229E-4 0.4540050416187062 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6168 0.45303773257846636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4949 0.36350255164248213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3602 0.2645658094597334 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1382 0.10150748158616091 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.344969724034797E-5 0.0 3 0.0 0.0 0.0 7.344969724034797E-5 0.0 4 0.0 0.0 0.0 7.344969724034797E-5 0.0 5 0.0 0.0 0.0 7.344969724034797E-5 0.0 6 0.0 0.0 0.0 7.344969724034797E-5 0.0 7 0.0 0.0 0.0 7.344969724034797E-5 0.0 8 0.0 0.0 0.0 7.344969724034797E-5 0.0 9 0.0 0.0 0.0 7.344969724034797E-5 0.0 10-11 0.0 0.0 0.0 1.4689939448069594E-4 0.0 12-13 0.0 0.0 0.0 2.2034909172104393E-4 0.0 14-15 0.0 0.0 0.0 2.2034909172104393E-4 0.0 16-17 0.0 0.0 0.0 2.2034909172104393E-4 0.0 18-19 0.0 0.0 0.0 2.2034909172104393E-4 0.0 20-21 0.0 0.0 0.0 2.570739403412179E-4 0.0 22-23 0.0 0.0 0.0 3.6724848620173987E-4 0.0 24-25 0.0 0.0 0.0 0.0011751951558455678 0.0 26-27 0.0 0.0 0.0 0.0019096921282490474 0.0 28-29 0.0 0.0 0.0 0.004443706683041053 0.0 30-31 0.0 0.0 0.0 0.0108705551915715 0.0 32-33 0.0 0.0 0.0 0.02515652130481918 0.0 34-35 0.0 0.0 0.0 0.04601623532107801 0.0 36-37 0.0 0.0 0.0 0.08439370212915982 0.0 38-39 0.0 0.0 0.0 0.14098669385284793 0.0 40-41 0.0 0.0 0.0 0.20378618499334544 0.0 42-43 0.0 0.0 0.0 0.26831174401899116 0.0 44-45 0.0 0.0 0.0 0.34682947036892314 0.0 46-47 0.0 0.0 0.0 0.4435994464830816 0.0 48-49 0.0 0.0 0.0 0.5458781498902662 0.0 50-51 0.0 0.0 0.0 0.655611997567346 0.0 52-53 0.0 0.0 0.0 0.7655294694875268 0.0 54-55 0.0 0.0 0.0 0.8880435644844271 0.0 56-57 0.0 0.0 0.0 1.0346491601761618 0.0 58-59 0.0 0.0 0.0 1.1958345207701053 0.0 60-61 0.0 0.0 0.0 1.3702041020186915 0.0 62-63 0.0 0.0 0.0 1.5437657365976336 0.0 64-65 0.0 0.0 0.0 1.7256639118133554 0.0 66-67 0.0 0.0 0.0 1.937492838654519 0.0 68-69 0.0 0.0 0.0 2.17414776316292 0.0 70-71 0.0 0.0 0.0 2.426374023486275 0.0 72-73 0.0 0.0 0.0 2.68495368262092 0.0 74-75 0.0 0.0 0.0 2.941770549021797 0.0 76-77 0.0 0.0 0.0 3.234320693130103 0.0 78-79 0.0 0.0 0.0 3.5547082724925008 0.0 80-81 0.0 0.0 0.0 3.900472722251439 0.0 82-83 0.0 0.0 0.0 4.257291351445049 0.0 84-85 0.0 0.0 0.0 4.61197993941869 0.0 86-87 0.0 0.0 0.0 4.978567378345266 0.0 88-89 0.0 0.0 0.0 5.373249326466276 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3210 0.0 49.029285 1 GTATCAA 5370 0.0 40.995796 1 ATCAACG 6880 0.0 31.858778 3 TCAACGC 7005 0.0 31.42603 4 TATCAAC 7090 0.0 30.982208 2 CAACGCA 7125 0.0 30.89675 5 AACGCAG 7465 0.0 29.597319 6 TGGTATC 1375 0.0 27.663298 2 GTACATG 5975 0.0 26.260841 1 TACATGG 5890 0.0 25.8316 2 CGCAGAG 8425 0.0 25.77552 8 GTGGTAT 1550 0.0 25.76797 1 ACGCAGA 8465 0.0 25.65372 7 GTACCGT 95 0.0012244404 25.007805 6 ACATGGG 6065 0.0 23.831942 3 GCAGAGT 8965 0.0 23.427025 9 CATGGGG 2755 0.0 22.60822 4 GAGTACT 5460 0.0 21.929922 12-13 AGTACTT 5565 0.0 21.302696 12-13 GAGTACA 4550 0.0 21.10924 1 >>END_MODULE