FastQCFastQC Report
Thu 26 May 2016
SRR938355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361476
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.45648986834876265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.386492306878711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.28931835742973067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14860.1091462500991571No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.10069953491651709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.047.2250751
GTATCAA53200.040.908691
ATCAACG69800.030.7633083
TATCAAC70800.030.4708372
TCAACGC71000.030.1764554
CAACGCA72150.029.695475
AACGCAG75400.028.4649036
TGGTATC14800.024.7224082
ACGCAGA88500.024.2514577
TACCTGG10650.024.0938342
CGCAGAG88750.024.0226358
TACATGG58950.023.2957022
GTACATG61450.023.2758941
GTGGTAT16800.022.062111
GCAGAGT95450.021.8886729
ACATGGG60350.021.883543
GAGTACT57150.021.06223112-13
AGTACTT57950.020.89437512-13
TAAGGTG6400.020.783955
GTACCTG13100.020.6759431
Produced by FastQC (version 0.11.5)