##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938355_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1361476 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820130505422057 31.0 31.0 34.0 30.0 34.0 2 32.03244640375593 31.0 31.0 34.0 30.0 34.0 3 32.090547317763956 33.0 31.0 34.0 30.0 34.0 4 35.761591096721496 37.0 35.0 37.0 35.0 37.0 5 35.665416063154986 37.0 35.0 37.0 33.0 37.0 6 35.666924719936304 37.0 35.0 37.0 33.0 37.0 7 35.61493628973261 37.0 35.0 37.0 33.0 37.0 8 35.59234683534635 37.0 35.0 37.0 33.0 37.0 9 37.17744565456901 39.0 37.0 39.0 33.0 39.0 10-11 37.07739504772761 39.0 37.0 39.0 33.0 39.0 12-13 36.96567108050381 39.0 37.0 39.0 33.0 39.0 14-15 37.98967333981649 40.0 37.0 41.0 33.0 41.0 16-17 37.89924170532569 40.0 37.0 41.0 33.0 41.0 18-19 38.15843724017169 40.0 37.5 41.0 33.0 41.0 20-21 38.14449244790213 40.0 37.0 41.0 33.0 41.0 22-23 38.02933617632628 40.0 37.0 41.0 33.0 41.0 24-25 37.90098944087153 40.0 37.0 41.0 32.5 41.0 26-27 37.704499748802036 40.0 37.0 41.0 32.0 41.0 28-29 37.57877663653271 39.5 36.5 41.0 32.0 41.0 30-31 37.31419099565471 39.0 36.0 41.0 31.5 41.0 32-33 37.09654485279212 39.0 36.0 41.0 31.0 41.0 34-35 36.970058230919975 39.0 36.0 41.0 31.0 41.0 36-37 36.91735219717424 39.0 35.5 41.0 31.0 41.0 38-39 36.748897152795934 39.0 35.0 41.0 30.0 41.0 40-41 36.581795786337764 39.0 35.0 41.0 30.0 41.0 42-43 36.35773858665155 39.0 35.0 40.0 30.0 41.0 44-45 36.03491761881958 39.0 35.0 40.0 29.5 41.0 46-47 35.62830817436371 38.0 35.0 40.0 27.5 41.0 48-49 35.252880329877286 38.0 34.0 40.0 27.0 41.0 50-51 34.878316253830405 38.0 34.0 40.0 26.0 41.0 52-53 34.39349279752269 38.0 33.0 40.0 24.0 41.0 54-55 34.055206995936764 38.0 33.0 40.0 23.5 41.0 56-57 33.72008100032612 37.0 33.0 40.0 23.0 41.0 58-59 33.24866101201931 37.0 32.0 39.5 22.0 41.0 60-61 32.85803495617991 36.0 31.0 39.0 20.5 40.5 62-63 32.38403394551207 36.0 31.0 39.0 18.5 40.0 64-65 31.858359603841713 35.0 30.5 38.5 17.5 40.0 66-67 31.346066328014594 35.0 30.0 38.0 13.5 40.0 68-69 30.838463917101734 34.5 29.0 38.0 9.5 40.0 70-71 30.261542252672832 34.0 29.0 37.0 7.5 39.0 72-73 29.748596743534222 34.0 28.0 36.5 7.0 39.0 74-75 29.144262917598255 34.0 27.5 36.0 3.5 39.0 76-77 27.927187478883212 31.5 26.0 34.5 2.0 37.0 78-79 28.598386603950416 33.0 27.0 35.0 2.0 37.0 80-81 28.2043436681954 33.0 26.5 35.0 2.0 37.0 82-83 27.751045556440218 33.0 26.0 35.0 2.0 36.0 84-85 27.170925892193473 32.5 25.5 35.0 2.0 36.0 86-87 26.539405395320962 32.0 24.5 34.5 2.0 35.5 88-89 25.774964450346538 31.5 22.5 34.0 2.0 35.0 90-91 25.141759017419332 31.0 20.0 34.0 2.0 35.0 92-93 24.28815785221333 31.0 17.0 34.0 2.0 35.0 94-95 23.458810878781556 30.5 4.5 34.0 2.0 35.0 96-97 22.3847067447388 30.0 2.0 34.0 2.0 35.0 98-99 21.016871395456107 29.0 2.0 34.0 2.0 35.0 100-101 17.072432051685084 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30689692439106153 1101 2 0.2882923808523614 1101 3 0.35365809768087075 1101 4 0.2943197223510481 1101 5 0.1707613647761761 1101 6 0.3054448643275407 1101 7 0.24572257599037073 1101 8 0.32498106616511535 1101 9 0.4246344455363982 1101 10-11 0.36430116796140766 1101 12-13 0.5137135376888082 1101 14-15 0.5202907420397125 1101 16-17 0.44453175487982577 1101 18-19 0.46066525511136547 1101 20-21 0.37831079969622294 1101 22-23 0.3616249894711743 1101 24-25 0.5123936352591798 1101 26-27 0.45996109567110466 1101 28-29 0.3874374997454382 1101 30-31 0.6160680604041247 1101 32-33 0.5208117999135737 1101 34-35 0.4459116029222372 1101 36-37 0.4998355212495511 1101 38-39 0.617222621961723 1101 40-41 0.7551584996987799 1101 42-43 0.7129131105609545 1101 44-45 0.7779899233362286 1101 46-47 0.588959914440089 1101 48-49 0.7631109472808291 1101 50-51 0.7763723933148512 1101 52-53 0.9011718296086073 1101 54-55 1.0089076171492266 1101 56-57 0.8406207126656113 1101 58-59 1.0933532021628949 1101 60-61 1.1301634704800776 1101 62-63 1.2003698460685897 1101 64-65 0.9111218095620792 1101 66-67 0.7731871776828889 1101 68-69 1.0220770564506232 1101 70-71 0.9445549587289364 1101 72-73 1.1075265547901374 1101 74-75 0.9269338845487702 1101 76-77 1.0634378797986095 1101 78-79 1.308492449897912 1101 80-81 1.159906516985373 1101 82-83 1.0239301144343145 1101 84-85 1.1519227816492652 1101 86-87 1.5843577587455755 1101 88-89 1.407759393194258 1101 90-91 1.350900297651993 1101 92-93 1.7744558121087834 1101 94-95 1.361013007746248 1101 96-97 1.1546449086458033 1101 98-99 1.2397392334014476 1101 100-101 1.36825143619782 1102 1 0.2829937360504218 1102 2 0.2874119342598789 1102 3 0.29549242385823504 1102 4 0.1814352294508197 1102 5 0.12692636421418513 1102 6 0.2761653319008843 1102 7 0.15430599362177588 1102 8 0.22146302637641924 1102 9 0.23869161836618957 1102 10-11 0.3375974101404182 1102 12-13 0.3614993081732436 1102 14-15 0.6268435126365333 1102 16-17 0.5589898119027836 1102 18-19 0.6092265679134385 1102 20-21 0.59557504607141 1102 22-23 0.5051190511399071 1102 24-25 0.6170918481399283 1102 26-27 0.5497385487451751 1102 28-29 0.5498611631525776 1102 30-31 0.4865674796840338 1102 32-33 0.5741537503837648 1102 34-35 0.5015670673109867 1102 36-37 0.672515450813826 1102 38-39 0.7285241842861012 1102 40-41 0.6231758089041293 1102 42-43 0.32531991435249097 1102 44-45 0.49248169959039956 1102 46-47 0.557451166172882 1102 48-49 0.5975682549875501 1102 50-51 0.8457403450588785 1102 52-53 0.6445029149931898 1102 54-55 0.6791335359982753 1102 56-57 0.7219290188362351 1102 58-59 0.9159151144179631 1102 60-61 0.9341610726680827 1102 62-63 0.8990510494704793 1102 64-65 0.8265240050586904 1102 66-67 0.790505749486119 1102 68-69 0.9122179279054201 1102 70-71 0.998252834417368 1102 72-73 0.9094448043873804 1102 74-75 0.8824994309876466 1102 76-77 0.9721617942266931 1102 78-79 1.2924383492647387 1102 80-81 1.2484475982487204 1102 82-83 1.126193049506064 1102 84-85 1.0561320656775912 1102 86-87 0.9725223085938381 1102 88-89 0.8922298139353337 1102 90-91 1.0090715913464976 1102 92-93 1.1086789584877117 1102 94-95 1.2994379449910163 1102 96-97 0.9782933294618097 1102 98-99 1.158822444970891 1102 100-101 1.6920309873573878 1103 1 0.18379418545824677 1103 2 0.15674218141086982 1103 3 0.1519744557037157 1103 4 0.16761617204176815 1103 5 0.029236105967854087 1103 6 0.007517121723473963 1103 7 0.1425568228584595 1103 8 0.21524287714878199 1103 9 0.22360685324471063 1103 10-11 0.2424251643367228 1103 12-13 0.1908612796004192 1103 14-15 0.3729729644662214 1103 16-17 0.423364452061854 1103 18-19 0.3931891421251663 1103 20-21 0.3527409796532197 1103 22-23 0.1892346524443056 1103 24-25 0.24836706614235027 1103 26-27 0.3821878679902255 1103 28-29 0.2753138925824956 1103 30-31 0.19960963031490309 1103 32-33 0.20450183165944935 1103 34-35 0.4430426540702399 1103 36-37 0.4387697718187482 1103 38-39 0.42995177671423335 1103 40-41 0.49476766362046476 1103 42-43 0.567032792170906 1103 44-45 0.6184522503247649 1103 46-47 0.6485896970661713 1103 48-49 0.7144982028441191 1103 50-51 0.66816598181709 1103 52-53 0.5924171778641423 1103 54-55 0.6730934529230908 1103 56-57 0.8103764639947286 1103 58-59 0.7995515882448458 1103 60-61 0.9097128676187722 1103 62-63 0.6083461924628253 1103 64-65 0.6380693339445607 1103 66-67 0.645336883564898 1103 68-69 0.7595527157536957 1103 70-71 0.8000174827114606 1103 72-73 0.7343826262046385 1103 74-75 0.825004716424548 1103 76-77 0.8949751850879579 1103 78-79 1.058237551612475 1103 80-81 1.0824842967795227 1103 82-83 1.1647196305335008 1103 84-85 1.2148892246183145 1103 86-87 1.0464176114895842 1103 88-89 1.0963438301913975 1103 90-91 0.9978148344952444 1103 92-93 1.4674890300712917 1103 94-95 1.5110809408256003 1103 96-97 1.1337883190774107 1103 98-99 1.2625347214033162 1103 100-101 1.2628465180339 1104 1 0.23809004805509915 1104 2 0.3252978279836256 1104 3 0.2941806230888204 1104 4 0.23206530163384542 1104 5 0.20284768335559988 1104 6 0.21651238136868756 1104 7 0.32234563311445896 1104 8 0.27563428094733666 1104 9 0.36020651036352547 1104 10-11 0.27239649391894716 1104 12-13 0.30196530330666604 1104 14-15 0.37198203336026836 1104 16-17 0.4106339808464128 1104 18-19 0.41380733541884496 1104 20-21 0.4287707422773792 1104 22-23 0.44441088263128137 1104 24-25 0.46066300483554556 1104 26-27 0.44898647229369715 1104 28-29 0.5495349121840221 1104 30-31 0.5860621305275302 1104 32-33 0.6318203083001137 1104 34-35 0.7471745738020132 1104 36-37 0.5467711907918016 1104 38-39 0.5083455618808159 1104 40-41 0.5017679680058578 1104 42-43 0.7437928559127869 1104 44-45 0.8064370847704438 1104 46-47 0.7293497429030396 1104 48-49 1.0102774294366625 1104 50-51 1.1741731169686673 1104 52-53 1.0771150977481128 1104 54-55 0.8748892148041989 1104 56-57 0.78995914267567 1104 58-59 0.96505666655694 1104 60-61 1.1398198277487666 1104 62-63 1.0742886009569688 1104 64-65 0.8434074926815391 1104 66-67 0.8387654044757262 1104 68-69 0.8713583925411719 1104 70-71 0.8735383040148577 1104 72-73 0.9419611509202852 1104 74-75 1.2775485725397822 1104 76-77 1.2045306481342308 1104 78-79 1.2786321559338525 1104 80-81 1.1464499907558228 1104 82-83 1.3041527575114458 1104 84-85 1.565445511295632 1104 86-87 1.5630586515923568 1104 88-89 1.736081342557057 1104 90-91 1.7109444758128731 1104 92-93 2.027476451792772 1104 94-95 2.0072207298149074 1104 96-97 1.9930374943273002 1104 98-99 2.1565828680096466 1104 100-101 2.3195237486640217 1105 1 0.14437055414515143 1105 2 0.17872716444758652 1105 3 0.09671277477972495 1105 4 0.01790111799010674 1105 5 0.047066972030677334 1105 6 0.04826072726068986 1105 7 0.19516487967916873 1105 8 0.07574189633080408 1105 9 0.1351882731715648 1105 10-11 0.15926077092859714 1105 12-13 0.17246369439288145 1105 14-15 0.41115238468408677 1105 16-17 0.23404798318162534 1105 18-19 0.2731291989940132 1105 20-21 0.18206114643056992 1105 22-23 0.15476625797715116 1105 24-25 0.1646433384432342 1105 26-27 0.06955637656704994 1105 28-29 0.1385403844049975 1105 30-31 0.3090761375416733 1105 32-33 0.46419951215894883 1105 34-35 0.27862681788050736 1105 36-37 0.477826895576861 1105 38-39 0.18303180774156402 1105 40-41 0.16688234656954393 1105 42-43 0.23110099320960842 1105 44-45 0.2872492193619891 1105 46-47 0.22655487994250478 1105 48-49 0.40955735278728156 1105 50-51 0.5090579408601386 1105 52-53 0.28646104717739007 1105 54-55 0.4316943458886655 1105 56-57 0.47275216596609937 1105 58-59 0.46177822557632453 1105 60-61 0.5789009603900439 1105 62-63 0.425832541423965 1105 64-65 0.481801726538702 1105 66-67 0.4317833247765357 1105 68-69 0.6317301973107057 1105 70-71 0.6472341586958059 1105 72-73 0.7160996429863111 1105 74-75 0.6822185697536902 1105 76-77 0.6048800791125721 1105 78-79 0.9729531581605961 1105 80-81 0.959858527250411 1105 82-83 1.1339721082886776 1105 84-85 1.16563389138636 1105 86-87 0.9679256423812461 1105 88-89 1.2520168201162711 1105 90-91 1.1044395406054157 1105 92-93 1.1080928157114158 1105 94-95 1.1631665329212915 1105 96-97 1.3206511019309843 1105 98-99 1.171164114433985 1105 100-101 1.0337065416873124 1106 1 -0.09019250150047498 1106 2 0.14074159357964078 1106 3 -0.025470415635837185 1106 4 0.003150903626675472 1106 5 -0.14573052236360695 1106 6 -0.10056700494079251 1106 7 0.03941192580128927 1106 8 0.08109767615614771 1106 9 -0.22443131570555153 1106 10-11 0.02032126294095349 1106 12-13 -0.06519989492644385 1106 14-15 0.11949830299800368 1106 16-17 0.09526055289271795 1106 18-19 -0.058829969069563504 1106 20-21 0.07946291878374723 1106 22-23 0.04533484872204241 1106 24-25 -0.04749156267451582 1106 26-27 -0.05482122892065178 1106 28-29 -0.011380191363805636 1106 30-31 0.14954266431448815 1106 32-33 0.2530204524381716 1106 34-35 0.12591383107979937 1106 36-37 0.18541507022659687 1106 38-39 -0.14240044997486478 1106 40-41 -0.09748899596179683 1106 42-43 0.11471976536335404 1106 44-45 -0.025274154319099296 1106 46-47 -0.7183258144416271 1106 48-49 -0.28500080463403066 1106 50-51 0.027021358635828108 1106 52-53 0.15669618839277177 1106 54-55 0.18609838380282184 1106 56-57 0.2687236673988025 1106 58-59 0.1581475622689439 1106 60-61 0.20752007901646863 1106 62-63 0.03138372125597044 1106 64-65 0.3149283223627286 1106 66-67 0.2767132658867588 1106 68-69 -0.0840897448098481 1106 70-71 0.046894350996073086 1106 72-73 0.5357677591958883 1106 74-75 0.5403332506206233 1106 76-77 0.29712744896183096 1106 78-79 0.587844818008012 1106 80-81 -0.3420979589560069 1106 82-83 -0.05401787944460068 1106 84-85 0.4422407443512988 1106 86-87 0.5244543776607884 1106 88-89 0.42028642980819697 1106 90-91 0.629599804051594 1106 92-93 0.5643425743707198 1106 94-95 0.8690075331746634 1106 96-97 0.7605285722678978 1106 98-99 0.7274788830034673 1106 100-101 0.6240938558153033 1107 1 0.0508538329427779 1107 2 0.06728474961656161 1107 3 0.07014035657068263 1107 4 0.03408687887666417 1107 5 -0.033245721764352254 1107 6 0.15964314441094984 1107 7 0.08166035968582008 1107 8 0.029445366616826618 1107 9 0.21557709847747475 1107 10-11 0.1666210746479564 1107 12-13 0.15541023595513792 1107 14-15 0.1769756385751151 1107 16-17 0.12644869710360496 1107 18-19 0.14636040182146814 1107 20-21 0.059601342355769305 1107 22-23 0.1679266736249403 1107 24-25 0.19238268633399258 1107 26-27 0.266064158619173 1107 28-29 0.2048776520400324 1107 30-31 0.1850405120280314 1107 32-33 0.2548461762374856 1107 34-35 0.29486677835262753 1107 36-37 0.33747038834940923 1107 38-39 0.27107840425967566 1107 40-41 0.43161253346988104 1107 42-43 0.1834461966723211 1107 44-45 0.3443932289212839 1107 46-47 0.1521817044518059 1107 48-49 0.005896485896421666 1107 50-51 0.17836685833055554 1107 52-53 0.14157143786817272 1107 54-55 0.02375826610687426 1107 56-57 0.33108276831256944 1107 58-59 0.06945565989398972 1107 60-61 0.18151640550728843 1107 62-63 0.16325243947832746 1107 64-65 0.10514914336124903 1107 66-67 0.1715123772669429 1107 68-69 0.2938948531307304 1107 70-71 0.44951608729352444 1107 72-73 0.5350702542063779 1107 74-75 0.4861887524189008 1107 76-77 0.36212290838135885 1107 78-79 0.7078836566455315 1107 80-81 0.4645895208422566 1107 82-83 0.48439183996291746 1107 84-85 0.7270659704824673 1107 86-87 0.8397615209943439 1107 88-89 0.7979488261059551 1107 90-91 0.9411056862838194 1107 92-93 1.3613316821081796 1107 94-95 1.310321684907013 1107 96-97 1.078320721424685 1107 98-99 1.1766841531551755 1107 100-101 0.8956526345274192 1108 1 0.15092572366887325 1108 2 0.10692921729841487 1108 3 0.1091182011560008 1108 4 0.02785852778850284 1108 5 0.08741959422806644 1108 6 0.11018886037722808 1108 7 0.14391772848524909 1108 8 0.09895062771087026 1108 9 0.1414381533104958 1108 10-11 0.09634135436823499 1108 12-13 0.1792256728546917 1108 14-15 0.22663579150628976 1108 16-17 0.274925920814475 1108 18-19 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-1.6846200042626691 2316 94-95 -1.771273167346525 2316 96-97 -2.02399702071342 2316 98-99 -2.025215776589061 2316 100-101 -2.212272540763024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 19.0 5 64.0 6 119.0 7 324.0 8 666.0 9 1248.0 10 1920.0 11 3036.0 12 4256.0 13 6165.0 14 7709.0 15 10017.0 16 12769.0 17 15710.0 18 15467.0 19 12949.0 20 12641.0 21 13382.0 22 14536.0 23 16513.0 24 18953.0 25 22430.0 26 26408.0 27 30935.0 28 36750.0 29 43291.0 30 51114.0 31 60844.0 32 72733.0 33 88406.0 34 112368.0 35 145027.0 36 186427.0 37 209617.0 38 102578.0 39 4077.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.53484463874395 19.60334108964415 15.309238823861232 22.55257544775067 2 15.884722734601553 21.000930366546783 39.72341804617146 23.390928852680204 3 19.32843472819205 25.09805534581587 29.927887087249427 25.64562283874266 4 13.162195413063039 17.57402816797958 36.52318257771901 32.740593841238365 5 13.511992198953148 37.599047061270106 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21.58153401829234 26.779360737569814 29.72703981948578 21.91206542465206 28-29 21.71091772593527 26.96709595629443 29.758812874869445 21.563173442900858 30-31 21.79342349197768 26.589038761557514 29.740123045101846 21.87741470136296 32-33 21.56580754625515 26.764049152754012 29.581922480847904 22.088220820142933 34-35 22.205102004461214 26.816982586718392 29.397001443852645 21.58091396496775 36-37 22.231817958074757 26.381253319834812 29.443876700141132 21.943052021949295 38-39 21.76404393692991 26.836153212193864 29.403241942521184 21.99656090835504 40-41 22.166314103976614 26.62799494660144 29.400533251068705 21.805157698353238 42-43 21.968544016782012 26.6307150128759 29.167811272914772 22.232929697427316 44-45 21.86976966605563 26.853868165375605 29.24182952283745 22.034532645731314 46-47 22.19660868057902 27.096805231968833 28.59198399384198 22.11460209361017 48-49 22.019190936894958 27.124716116920165 28.672117613531196 22.18397533265368 50-51 22.26205677783005 27.259506215549507 28.44796908035428 22.030467926266166 52-53 22.45640025971813 27.197578216582592 28.266418210824135 22.079603312875147 54-55 22.074406452804702 27.303409698965336 28.390188581403198 22.231995266826765 56-57 22.164254088944645 27.404118765222453 28.32818940620327 22.103437739629637 58-59 22.18457452572535 27.014324205685234 28.371257551018157 22.429843717571256 60-61 21.941995305095354 27.285093530844467 28.50120016805291 22.271710996007275 62-63 21.91652171102505 27.276660574612 28.61619027190936 22.19062744245359 64-65 21.769144000614133 27.166205638343943 28.452101844713223 22.612548516328705 66-67 21.765508700356477 27.313331095409733 28.268280518091554 22.65287968614223 68-69 21.98035461889048 27.10613736339621 28.423873597230752 22.489634420482563 70-71 21.821582540412958 27.11102770486872 28.32298487927252 22.744404875445795 72-73 21.945883652570668 27.157991999847226 28.1226082907202 22.7735160568619 74-75 21.77030724659402 27.481237655385566 27.935442327026628 22.813012770993787 76-77 21.832233903826587 27.185591514875828 28.16440405752136 22.817770523776225 78-79 21.82805582128021 27.298175390027463 28.229289273491652 22.644479515200675 80-81 21.797595799923297 27.35567379169183 28.064429388194117 22.782301020190754 82-83 21.862088778869886 27.317912058763667 28.014066365559447 22.805932796807 84-85 21.88576453398039 27.267691760680147 27.994785640456026 22.85175806488344 86-87 21.856789314512426 27.254810210361534 27.9378957088068 22.950504766319234 88-89 21.77374032165285 27.33394961102831 27.760577953308697 23.13173211401015 90-91 21.67901680824146 27.19789908002098 27.987323655994555 23.135760455743 92-93 21.69450358595944 27.05650843189028 27.9126645125931 23.33632346955718 94-95 21.555860946140196 26.86932650355577 28.005577847598833 23.569234702705206 96-97 21.61918393829021 26.75764961413012 27.944246339508183 23.678920108071488 98-99 21.434424241711632 26.816665956280378 27.785424637078343 23.963485164929647 100-101 21.429770422340177 26.603431158106773 28.155558763366745 23.811239656186306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1084.0 1 724.5 2 757.5 3 1015.0 4 682.5 5 575.5 6 929.5 7 1866.0 8 2726.0 9 2864.5 10 2357.5 11 1754.5 12 1606.5 13 1664.0 14 1756.5 15 1817.5 16 1897.5 17 2022.5 18 2158.0 19 2329.5 20 2607.0 21 2997.0 22 3429.0 23 4117.0 24 4972.5 25 5969.5 26 7463.5 27 9359.0 28 11222.0 29 13167.5 30 15603.5 31 18345.0 32 21946.5 33 24541.5 34 26153.0 35 28536.0 36 31390.0 37 34457.0 38 36457.0 39 37823.0 40 39940.0 41 42821.5 42 45188.5 43 47287.0 44 48987.5 45 50560.5 46 51583.5 47 52357.0 48 52986.0 49 51993.0 50 50538.0 51 48563.5 52 46143.5 53 45166.5 54 44891.5 55 41844.5 56 37171.0 57 33304.0 58 30388.0 59 26848.5 60 21890.5 61 17773.5 62 14688.0 63 11268.0 64 8407.0 65 6440.0 66 4744.5 67 3682.0 68 2829.0 69 2078.0 70 1595.5 71 1230.0 72 956.0 73 794.5 74 621.5 75 445.0 76 319.5 77 201.5 78 136.0 79 93.0 80 50.0 81 22.5 82 15.5 83 13.0 84 6.0 85 2.5 86 3.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.937987889613919E-4 2 0.05303068140753124 3 0.0 4 7.344969724034797E-5 5 0.04649365835314027 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006977721237833057 26-27 0.002350390311691135 28-29 1.4689939448069594E-4 30-31 0.0017627927337683512 32-33 4.406981834420878E-4 34-35 0.013771818232565246 36-37 0.024899447364477963 38-39 0.005141478806824358 40-41 0.0011751951558455675 42-43 0.0013220945503262635 44-45 0.00929138670090402 46-47 0.0 48-49 0.0 50-51 8.446715182640017E-4 52-53 0.0 54-55 0.0015791684906674814 56-57 0.0 58-59 0.02339372857105083 60-61 0.0 62-63 0.010282957613648716 64-65 0.015240812177372205 66-67 0.006977721237833057 68-69 0.017627927337683515 70-71 3.6724848620173986E-5 72-73 1.4689939448069594E-4 74-75 0.022438882506926307 76-77 0.051010814733421665 78-79 0.030701973446465452 80-81 0.02629499161204457 82-83 0.03665139892293364 84-85 0.017260678851481775 86-87 0.01123780367777324 88-89 0.08509147425294313 90-91 0.02629499161204457 92-93 0.02453219887827622 94-95 0.027727260708231363 96-97 0.03856109105118269 98-99 0.05244308382960845 100-101 0.015828409755294988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1361476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.35281419416171 #Duplication Level Percentage of deduplicated Percentage of total 1 82.60020119508513 51.503549975175176 2 10.529799793075986 13.131252999987788 3 2.8025494830620388 5.242405415619338 4 1.2259913155041418 3.0577603479714255 5 0.6746185237089369 2.1032181730381514 6 0.4205683585825919 1.573417243118636 7 0.2840361623127231 1.2397317837175583 8 0.2152893845873824 1.0739119196121982 9 0.1623883604791184 0.9112834141424121 >10 0.9819040396033352 11.273837551006192 >50 0.06874273491159226 2.944985430683198 >100 0.030967051897837612 3.5200616247214107 >500 0.0021193899762169247 0.9167534268434376 >1k 5.887194378380347E-4 0.6652280612339545 >5k 2.3548777513521387E-4 0.8426026331290251 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6215 0.45648986834876265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5262 0.386492306878711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3939 0.28931835742973067 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1486 0.1091462500991571 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.10069953491651709 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.344969724034797E-5 7 0.0 0.0 0.0 0.0 7.344969724034797E-5 8 0.0 0.0 0.0 0.0 7.344969724034797E-5 9 0.0 0.0 0.0 0.0 7.344969724034797E-5 10-11 0.0 0.0 0.0 0.0 7.344969724034797E-5 12-13 0.0 0.0 0.0 0.0 7.344969724034797E-5 14-15 0.0 0.0 0.0 0.0 7.344969724034797E-5 16-17 0.0 0.0 0.0 0.0 7.344969724034797E-5 18-19 0.0 0.0 0.0 0.0 7.344969724034797E-5 20-21 0.0 0.0 0.0 1.1017454586052195E-4 7.344969724034797E-5 22-23 0.0 0.0 0.0 2.203490917210439E-4 7.344969724034797E-5 24-25 0.0 0.0 0.0 0.0011017454586052197 7.344969724034797E-5 26-27 0.0 0.0 0.0 0.0018362424310086993 7.344969724034797E-5 28-29 0.0 0.0 0.0 0.004370256985800704 7.344969724034797E-5 30-31 0.0 0.0 0.0 0.010944004888811847 7.344969724034797E-5 32-33 0.0 0.0 0.0 0.02596446797446301 7.344969724034797E-5 34-35 0.0 0.0 0.0 0.0470078062338227 7.344969724034797E-5 36-37 0.0 0.0 0.0 0.08553217243638522 7.344969724034797E-5 38-39 0.0 0.0 0.0 0.1427494865866163 7.344969724034797E-5 40-41 0.0 0.0 0.0 0.206430374093998 7.344969724034797E-5 42-43 0.0 0.0 0.0 0.27055195978482177 7.344969724034797E-5 44-45 0.0 0.0 0.0 0.3488493370430327 7.344969724034797E-5 46-47 0.0 0.0 0.0 0.44631708528097447 7.344969724034797E-5 48-49 0.0 0.0 0.0 0.5494037353578028 7.344969724034797E-5 50-51 0.0 0.0 0.0 0.6600924290990073 7.344969724034797E-5 52-53 0.0 0.0 0.0 0.7696793773816064 7.344969724034797E-5 54-55 0.0 0.0 0.0 0.8932584929884919 7.344969724034797E-5 56-57 0.0 0.0 0.0 1.0403782365609089 7.344969724034797E-5 58-59 0.0 0.0 0.0 1.2013432480631314 7.344969724034797E-5 60-61 0.0 0.0 0.0 1.376447326284121 7.344969724034797E-5 62-63 0.0 0.0 0.0 1.5510372566244282 7.344969724034797E-5 64-65 0.0 0.0 0.0 1.7326049082025685 7.344969724034797E-5 66-67 0.0 0.0 0.0 1.9464537017178416 7.344969724034797E-5 68-69 0.0 0.0 0.0 2.1840634722903673 7.344969724034797E-5 70-71 0.0 0.0 0.0 2.4370242295861253 7.344969724034797E-5 72-73 0.0 0.0 0.0 2.700121045101052 7.344969724034797E-5 74-75 0.0 0.0 0.0 2.9613081684877294 7.344969724034797E-5 76-77 0.0 0.0 0.0 3.2596608386780233 7.344969724034797E-5 78-79 0.0 0.0 0.0 3.5867323404892923 7.344969724034797E-5 80-81 0.0 0.0 0.0 3.940539532096049 7.344969724034797E-5 82-83 0.0 0.0 0.0 4.3053641782888565 7.344969724034797E-5 84-85 0.0 0.0 0.0 4.66908707902306 7.344969724034797E-5 86-87 0.0 0.0 0.0 5.048014067086015 7.344969724034797E-5 88-89 0.0 0.0 0.0 5.455990410407528 7.344969724034797E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3240 0.0 47.225075 1 GTATCAA 5320 0.0 40.90869 1 ATCAACG 6980 0.0 30.763308 3 TATCAAC 7080 0.0 30.470837 2 TCAACGC 7100 0.0 30.176455 4 CAACGCA 7215 0.0 29.69547 5 AACGCAG 7540 0.0 28.464903 6 TGGTATC 1480 0.0 24.722408 2 ACGCAGA 8850 0.0 24.251457 7 TACCTGG 1065 0.0 24.093834 2 CGCAGAG 8875 0.0 24.022635 8 TACATGG 5895 0.0 23.295702 2 GTACATG 6145 0.0 23.275894 1 GTGGTAT 1680 0.0 22.06211 1 GCAGAGT 9545 0.0 21.888672 9 ACATGGG 6035 0.0 21.88354 3 GAGTACT 5715 0.0 21.062231 12-13 AGTACTT 5795 0.0 20.894375 12-13 TAAGGTG 640 0.0 20.78395 5 GTACCTG 1310 0.0 20.675943 1 >>END_MODULE