FastQCFastQC Report
Thu 26 May 2016
SRR938354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351460
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62650.4635727287526083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49060.36301481360898585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.26637858316191376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13720.10151983780504047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.047.1086241
GTATCAA53550.040.192561
TATCAAC68950.030.7429892
ATCAACG70200.030.1955683
TGGTATC15100.029.586692
TCAACGC71900.029.4816274
CAACGCA72700.029.0253855
GTGGTAT16900.028.7395611
AACGCAG75200.028.0531716
GTACATG57550.025.2360381
ACGCAGA83950.025.0698437
TACATGG57450.024.4876542
CGCAGAG86000.024.4170078
TAAGGTG5300.024.2112545
GCAGAGT90750.022.6146379
ACATGGG61400.022.6026823
GGACCGA4600.021.6909356
GAGTACT54350.021.67486412-13
GTATACG1353.7815803E-421.1233584
GTAAGGT5700.020.8454194
Produced by FastQC (version 0.11.5)