##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.963330768206237 31.0 31.0 34.0 28.0 34.0 2 31.045940686368816 31.0 31.0 34.0 27.0 34.0 3 31.10752223521229 31.0 31.0 34.0 27.0 34.0 4 34.73978586121676 37.0 35.0 37.0 32.0 37.0 5 34.76729166974975 37.0 35.0 37.0 32.0 37.0 6 34.79398576354461 37.0 35.0 37.0 32.0 37.0 7 34.72474065085167 37.0 35.0 37.0 31.0 37.0 8 34.67832640255723 37.0 35.0 37.0 30.0 37.0 9 36.16428973110562 39.0 35.0 39.0 31.0 39.0 10-11 36.01154418184778 39.0 35.0 39.0 30.0 39.0 12-13 35.855013096947005 38.0 35.0 39.0 30.0 39.0 14-15 36.80266785550442 39.0 36.0 41.0 30.5 41.0 16-17 36.657930682373134 39.0 36.0 41.0 30.0 41.0 18-19 36.668849614490995 39.0 36.0 41.0 30.0 41.0 20-21 36.763937519423436 39.0 36.0 41.0 30.0 41.0 22-23 36.66732089739985 39.0 36.0 41.0 30.0 41.0 24-25 36.54504313853167 39.0 36.0 41.0 30.0 41.0 26-27 36.344232903674545 39.0 35.5 41.0 29.0 41.0 28-29 36.14666064848386 39.0 35.0 40.5 28.5 41.0 30-31 35.904761147203764 38.5 35.0 40.0 27.0 41.0 32-33 35.600419176298224 38.0 34.5 40.0 27.0 41.0 34-35 35.327609770174476 38.0 34.0 40.0 26.0 41.0 36-37 35.03067904340491 38.0 34.0 40.0 25.0 41.0 38-39 34.76213428440353 38.0 34.0 40.0 24.5 41.0 40-41 34.67293075636719 38.0 33.5 40.0 24.0 41.0 42-43 34.47370510410963 38.0 33.0 40.0 24.0 41.0 44-45 34.15497055036775 38.0 33.0 40.0 23.0 41.0 46-47 33.76087083598479 38.0 33.0 40.0 20.5 41.0 48-49 33.37589495804537 38.0 32.5 40.0 18.5 41.0 50-51 32.64055909904844 37.0 31.5 39.5 15.5 40.5 52-53 32.703742989063684 37.0 31.5 39.5 15.0 40.0 54-55 32.83250447664008 37.0 32.0 40.0 14.0 41.0 56-57 32.53109895964364 37.0 31.0 40.0 11.0 41.0 58-59 32.20328607579951 36.5 31.0 40.0 8.0 41.0 60-61 31.762088778062243 36.0 30.5 39.0 7.5 41.0 62-63 31.237030322762052 35.5 29.5 39.0 3.5 40.0 64-65 30.736463528332322 35.0 29.0 39.0 2.0 40.0 66-67 30.199702173945216 35.0 28.5 38.0 2.0 40.0 68-69 29.650127639737768 34.5 28.0 38.0 2.0 40.0 70-71 29.03290219466355 34.0 26.5 37.0 2.0 39.0 72-73 28.476256418983915 34.0 26.0 36.5 2.0 39.0 74-75 27.86607261776153 33.5 26.0 36.0 2.0 39.0 76-77 27.135646634010627 33.0 24.5 35.5 2.0 38.0 78-79 26.353505468160357 32.0 22.0 35.0 2.0 37.0 80-81 25.69611901203143 32.0 20.0 35.0 2.0 36.5 82-83 25.028574652597932 31.0 18.0 35.0 2.0 36.0 84-85 24.370639900551996 31.0 13.0 34.0 2.0 35.5 86-87 23.69984387255265 30.5 7.0 34.0 2.0 35.0 88-89 22.920416808488596 30.0 2.0 34.0 2.0 35.0 90-91 22.084047622571145 29.0 2.0 34.0 2.0 35.0 92-93 21.24188100276738 29.0 2.0 34.0 2.0 35.0 94-95 20.29836399153508 28.0 2.0 34.0 2.0 35.0 96-97 19.089030751927545 25.5 2.0 33.0 2.0 35.0 98-99 17.657222189335975 22.5 2.0 33.0 2.0 34.5 100-101 14.341326417356044 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5019625651681245 1101 2 0.4927663514020857 1101 3 0.6266161977405389 1101 4 0.5136127365425054 1101 5 0.48114614461103145 1101 6 0.5825217260447246 1101 7 0.5299855545861405 1101 8 0.44990453842119393 1101 9 0.42848695512772395 1101 10-11 0.46447698225921386 1101 12-13 0.5042476221310466 1101 14-15 0.7612076734624509 1101 16-17 0.715725833748877 1101 18-19 0.5715867617876143 1101 20-21 0.6486456068871647 1101 22-23 0.731168498051332 1101 24-25 0.962926281854827 1101 26-27 0.7829807736457894 1101 28-29 0.9182556208550281 1101 30-31 0.5059492297617965 1101 32-33 0.7615951871837225 1101 34-35 0.8603628026225607 1101 36-37 0.6430322828890596 1101 38-39 0.6143909398807423 1101 40-41 0.6718343436735594 1101 42-43 0.9848604727447849 1101 44-45 0.8846099791112323 1101 46-47 0.7592741850673974 1101 48-49 0.6923937234368651 1101 50-51 0.8088253898463833 1101 52-53 0.6696263022855291 1101 54-55 0.6897957924812701 1101 56-57 0.6706028706919724 1101 58-59 0.6955188545272719 1101 60-61 0.6181230159356943 1101 62-63 0.3244688591404312 1101 64-65 0.48118295183443394 1101 66-67 0.6405919393039703 1101 68-69 0.6845571610198924 1101 70-71 0.818566527433692 1101 72-73 0.7847436661163734 1101 74-75 0.3461491139872166 1101 76-77 0.5607921425631694 1101 78-79 0.3594610090765542 1101 80-81 0.40707226005801544 1101 82-83 0.41838646970862925 1101 84-85 0.6273710845461373 1101 86-87 0.4673460319699636 1101 88-89 0.47755597830145646 1101 90-91 0.7034928042758963 1101 92-93 0.8337290817096594 1101 94-95 0.5951166459632873 1101 96-97 0.816168386557429 1101 98-99 0.7697007228775163 1101 100-101 0.7331228353048793 1102 1 0.5648659489629431 1102 2 0.6627011654023818 1102 3 0.5110053673220918 1102 4 0.5613157436930578 1102 5 0.47931474347541325 1102 6 0.4543887377579807 1102 7 0.4216921133151601 1102 8 0.488745030207383 1102 9 0.5565561161147059 1102 10-11 0.5234957481172913 1102 12-13 0.459292664614118 1102 14-15 0.5696635267734109 1102 16-17 0.5412219962114762 1102 18-19 0.5585195440575248 1102 20-21 0.5851500826975595 1102 22-23 0.5813816243567089 1102 24-25 0.4956507280266109 1102 26-27 0.7022231246390618 1102 28-29 0.40793424092144903 1102 30-31 0.45473919686862274 1102 32-33 0.4300461744530182 1102 34-35 0.33020914313804184 1102 36-37 0.36479770808314527 1102 38-39 0.8030116698501857 1102 40-41 0.7493554430198017 1102 42-43 0.9673395629845771 1102 44-45 0.8908119703965909 1102 46-47 0.9004010844825245 1102 48-49 0.7730998682363577 1102 50-51 1.1722292807838528 1102 52-53 0.977926379067874 1102 54-55 0.8908650577405837 1102 56-57 1.145120611205563 1102 58-59 0.8400450316195887 1102 60-61 0.6630985119401629 1102 62-63 0.7035881687944681 1102 64-65 0.6825394399426479 1102 66-67 0.44305971757677653 1102 68-69 0.39241487037817535 1102 70-71 0.6487021762445124 1102 72-73 0.6528458150741194 1102 74-75 0.5895728482634226 1102 76-77 0.6693494244049347 1102 78-79 0.6063358412139195 1102 80-81 0.7624547656903005 1102 82-83 0.8692637791035658 1102 84-85 0.9855029037189773 1102 86-87 0.9744915297755021 1102 88-89 0.8662784527784346 1102 90-91 0.92258727815884 1102 92-93 0.9267270373402035 1102 94-95 1.0274398016861 1102 96-97 1.1934651424243263 1102 98-99 1.3478458762684227 1102 100-101 1.1624189339401756 1103 1 0.6724304690168275 1103 2 0.5846624083851992 1103 3 0.5726992251687477 1103 4 0.42212475853685305 1103 5 0.5286398801055796 1103 6 0.40371224406461437 1103 7 0.5331977895582654 1103 8 0.445218410948911 1103 9 0.5252164671897859 1103 10-11 0.6150981931195432 1103 12-13 0.4570491582833327 1103 14-15 0.6849560822934748 1103 16-17 0.7108532351426362 1103 18-19 0.5695710242403393 1103 20-21 0.7945584986691827 1103 22-23 0.8035757016932195 1103 24-25 0.916181513063627 1103 26-27 0.8094495035153884 1103 28-29 0.7704348673883601 1103 30-31 0.743002739271823 1103 32-33 0.9426528021408629 1103 34-35 0.994376986757409 1103 36-37 0.9954174182116233 1103 38-39 0.808727765309861 1103 40-41 0.7626809226217404 1103 42-43 0.8080611804104407 1103 44-45 0.756066334078298 1103 46-47 0.592723407898859 1103 48-49 0.577732160839588 1103 50-51 0.6191568153180356 1103 52-53 0.780709160194526 1103 54-55 0.7821100995946111 1103 56-57 0.916942642673213 1103 58-59 0.9076382453676857 1103 60-61 1.2267629478814328 1103 62-63 1.2556607024923458 1103 64-65 1.1746299555052993 1103 66-67 1.1741924965598542 1103 68-69 1.2469827652017784 1103 70-71 0.969016369780821 1103 72-73 1.0963840881267437 1103 74-75 0.8967043664629664 1103 76-77 0.8748197033049863 1103 78-79 1.0453668735166666 1103 80-81 0.8136218660029044 1103 82-83 0.9355590741709925 1103 84-85 0.9843803957741706 1103 86-87 1.057928469163091 1103 88-89 1.087298093346437 1103 90-91 1.1186650820285031 1103 92-93 1.1665298164983895 1103 94-95 1.1682614122887571 1103 96-97 1.2458148311231056 1103 98-99 1.1949532294502418 1103 100-101 1.1216596379259531 1104 1 0.4852054466037785 1104 2 0.4898465641449512 1104 3 0.5482522916657899 1104 4 0.6094736577046334 1104 5 0.5538387167011933 1104 6 0.49920105597706765 1104 7 0.5560953993706903 1104 8 0.5045899467210617 1104 9 0.570422263930503 1104 10-11 0.5432225953421792 1104 12-13 0.44212344976590856 1104 14-15 0.7585547193034685 1104 16-17 0.597652640073278 1104 18-19 0.6358211769256386 1104 20-21 0.7377497712925347 1104 22-23 0.7929326718636958 1104 24-25 0.7051465141353006 1104 26-27 0.7176527950648008 1104 28-29 0.886412030276496 1104 30-31 0.8611077014865316 1104 32-33 0.9439353586805268 1104 34-35 0.9051681501387492 1104 36-37 0.9264307415887245 1104 38-39 0.8331081510678331 1104 40-41 0.91493467291847 1104 42-43 0.9217567063277627 1104 44-45 0.8941906441135856 1104 46-47 1.2380478382386357 1104 48-49 1.3473441557969323 1104 50-51 1.18404518795775 1104 52-53 1.1860387991197072 1104 54-55 1.1632780096381978 1104 56-57 1.2032388198941888 1104 58-59 1.2081595072281601 1104 60-61 1.3441641172779981 1104 62-63 1.1863415752300135 1104 64-65 1.3241575529415677 1104 66-67 1.3603158801122497 1104 68-69 1.4330638001891813 1104 70-71 1.5412048283852435 1104 72-73 1.4567415676323456 1104 74-75 1.1292273729690905 1104 76-77 1.323192193216233 1104 78-79 1.3946359430466728 1104 80-81 1.4901061976100252 1104 82-83 1.2412572320372206 1104 84-85 1.081781519077058 1104 86-87 1.4534897057642375 1104 88-89 1.5535627079226408 1104 90-91 1.5356656397619837 1104 92-93 1.344208838574957 1104 94-95 1.1802979115662389 1104 96-97 0.9017494495481309 1104 98-99 0.9107713115384684 1104 100-101 0.784470568580323 1105 1 0.6403806001590588 1105 2 0.5916794985912759 1105 3 0.47333612279713577 1105 4 0.536451391377831 1105 5 0.49551203148465817 1105 6 0.639690357373496 1105 7 0.4855069923891904 1105 8 0.5801239040044806 1105 9 0.6471204523997471 1105 10-11 0.5991851453652046 1105 12-13 0.6843208307321831 1105 14-15 0.6630691149085806 1105 16-17 0.6939506119112053 1105 18-19 0.6861790401884704 1105 20-21 0.7031617275252202 1105 22-23 0.6365073480517651 1105 24-25 0.7748374253488919 1105 26-27 0.8892390681629507 1105 28-29 0.9031628523728941 1105 30-31 0.7059917323856837 1105 32-33 0.881952209224373 1105 34-35 0.7847731871411909 1105 36-37 0.9578795770332391 1105 38-39 0.9192345042028265 1105 40-41 0.942566516425039 1105 42-43 1.057609351629651 1105 44-45 1.0892982787460568 1105 46-47 1.1302275449431818 1105 48-49 0.7892858274902252 1105 50-51 0.876600594336054 1105 52-53 0.8399013549801069 1105 54-55 0.9353100308602791 1105 56-57 1.0630859780674768 1105 58-59 1.0599137993172292 1105 60-61 1.1638558392375415 1105 62-63 1.1455059231632667 1105 64-65 1.1630534142456348 1105 66-67 1.1634878425714668 1105 68-69 0.9251549734358662 1105 70-71 1.0571927385416195 1105 72-73 0.9643285282430618 1105 74-75 1.1745642865565458 1105 76-77 1.2824676133447852 1105 78-79 1.1260617533061748 1105 80-81 1.3173986628614571 1105 82-83 1.3401998571677538 1105 84-85 1.281070981325481 1105 86-87 1.3894205069758172 1105 88-89 1.4578833352454303 1105 90-91 1.3343436374140651 1105 92-93 1.2474981970479 1105 94-95 1.5470331673403734 1105 96-97 1.5391943998980473 1105 98-99 1.6915991155844132 1105 100-101 1.5306941139399388 1106 1 0.5236939350922647 1106 2 0.5745512688496532 1106 3 0.3136576970711964 1106 4 0.5375217057526811 1106 5 0.6644393173017988 1106 6 0.2730348898109014 1106 7 0.4330823863576754 1106 8 0.5894047615358815 1106 9 0.4822040457122867 1106 10-11 0.2930624351820157 1106 12-13 0.41875007639253425 1106 14-15 0.6364776422263887 1106 16-17 0.38124122866186383 1106 18-19 0.1855358916403631 1106 20-21 0.32588790943066925 1106 22-23 0.27271245164347135 1106 24-25 0.675041409030193 1106 26-27 0.2898750172870237 1106 28-29 0.4056362545007275 1106 30-31 0.5298314252102543 1106 32-33 0.6019834067285714 1106 34-35 0.4681361181067203 1106 36-37 0.22916347432146011 1106 38-39 0.303333376293061 1106 40-41 0.6583180563018516 1106 42-43 0.41186181143287115 1106 44-45 0.8142306841536211 1106 46-47 0.33256735775815827 1106 48-49 0.6462580697108464 1106 50-51 0.3440551979677622 1106 52-53 0.8133210814019947 1106 54-55 0.5725222538824326 1106 56-57 0.17448506614042714 1106 58-59 -0.32829194422329167 1106 60-61 0.48055050366438223 1106 62-63 0.5258610947495335 1106 64-65 0.6856140093980194 1106 66-67 -0.05800243820607065 1106 68-69 -0.07402829190290916 1106 70-71 0.1500837072641268 1106 72-73 0.3137485746393516 1106 74-75 0.33972787346959166 1106 76-77 0.6731571581811977 1106 78-79 0.7386399470506753 1106 80-81 0.6568179093217381 1106 82-83 0.687868843648836 1106 84-85 0.6086383759339107 1106 86-87 0.8944857017602352 1106 88-89 0.7470562014161359 1106 90-91 0.4868618359581838 1106 92-93 0.3438584882246083 1106 94-95 0.5084410547093832 1106 96-97 0.5774401402388243 1106 98-99 0.3206511914183494 1106 100-101 -0.30845235434260054 1107 1 0.3459860385164433 1107 2 0.4283038893947264 1107 3 0.3362572777839823 1107 4 0.4035035366401729 1107 5 0.3244253410614064 1107 6 0.3648308367011879 1107 7 0.3591201222067326 1107 8 0.21999875175251304 1107 9 0.37963241748028054 1107 10-11 0.4601064603392473 1107 12-13 0.47493456276570356 1107 14-15 0.6190213179776194 1107 16-17 0.6360141147555041 1107 18-19 0.4599873242049952 1107 20-21 0.6458040519883284 1107 22-23 0.7215594118111923 1107 24-25 0.7802475585759723 1107 26-27 0.6282048565602523 1107 28-29 0.5565656744867411 1107 30-31 0.6927105307308707 1107 32-33 0.7446039518019418 1107 34-35 0.7058579909229294 1107 36-37 0.4657672479063635 1107 38-39 0.7374913078094849 1107 40-41 0.6898274902641148 1107 42-43 0.8012328806152595 1107 44-45 0.7270329203520802 1107 46-47 0.7721550586666126 1107 48-49 0.6593188663565499 1107 50-51 0.45541089328571616 1107 52-53 0.6055502917444144 1107 54-55 0.6539408422821609 1107 56-57 0.6600895385373562 1107 58-59 0.654419446412625 1107 60-61 0.7614729355302075 1107 62-63 0.8466717166922315 1107 64-65 0.5955161757152823 1107 66-67 0.4708934388030137 1107 68-69 0.5224007786771026 1107 70-71 0.6538363278091985 1107 72-73 0.4855864502508105 1107 74-75 0.6674637225261932 1107 76-77 0.783702231556461 1107 78-79 0.77075734746996 1107 80-81 0.6886278324524184 1107 82-83 0.7126696822232574 1107 84-85 1.0010073486802433 1107 86-87 1.0154360861068419 1107 88-89 0.9396138534077529 1107 90-91 1.07244534399452 1107 92-93 1.140783715338511 1107 94-95 0.9344648143180478 1107 96-97 1.0446588684009477 1107 98-99 1.0626637700346997 1107 100-101 0.639325423435178 1108 1 0.3725543339526638 1108 2 0.308511382809769 1108 3 0.36320185661535476 1108 4 0.2942738425048219 1108 5 0.278370941233419 1108 6 0.3266246334006411 1108 7 0.2930823863576748 1108 8 0.22894892108003972 1108 9 0.2261356696439094 1108 10-11 0.21995702207660628 1108 12-13 0.276371159013614 1108 14-15 0.48204744279619405 1108 16-17 0.3279791203997604 1108 18-19 0.3387837548882189 1108 20-21 0.28721269575545705 1108 22-23 0.2543363832674075 1108 24-25 0.30125223524102296 1108 26-27 0.2874533648653639 1108 28-29 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-1.1161253683806933 2316 58-59 -1.2255565159668862 2316 60-61 -1.2902984914741928 2316 62-63 -1.380667246853232 2316 64-65 -1.3367931474205044 2316 66-67 -1.4678129930098578 2316 68-69 -1.2010833713395854 2316 70-71 -1.5493068605702867 2316 72-73 -1.2595762386719294 2316 74-75 -0.9997514028297125 2316 76-77 -0.8180987933047952 2316 78-79 -0.7066950902870701 2316 80-81 -0.7926378116224804 2316 82-83 -0.7767795801470854 2316 84-85 -0.8765523222067166 2316 86-87 -0.6686982805983099 2316 88-89 -0.9131502838241765 2316 90-91 -1.2014126089874395 2316 92-93 -1.2417577098408508 2316 94-95 -1.4131461752810957 2316 96-97 -1.6950918098438699 2316 98-99 -1.5828640694446818 2316 100-101 -1.1340044016537814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5139.0 3 1858.0 4 1942.0 5 2215.0 6 2498.0 7 3231.0 8 4124.0 9 5223.0 10 6499.0 11 7985.0 12 9650.0 13 11288.0 14 12609.0 15 14542.0 16 16427.0 17 19098.0 18 17894.0 19 16479.0 20 17206.0 21 18707.0 22 20270.0 23 22449.0 24 24930.0 25 27726.0 26 31127.0 27 35194.0 28 40000.0 29 45694.0 30 52506.0 31 61300.0 32 71847.0 33 86674.0 34 105782.0 35 131736.0 36 155125.0 37 159321.0 38 80653.0 39 4512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79568320577866 18.757654021901253 14.746056974092712 22.70060579822738 2 15.356363943256573 20.28040053835482 40.89231274920749 23.47092276918111 3 19.254766441762573 24.9803449516508 29.493899161828786 26.27098944475784 4 12.657045174224823 16.78319659205494 37.050075292842514 33.509682940877724 5 13.601797096599125 37.47115220161363 33.94418723803756 14.982863463749691 6 31.506435483381324 36.07646907687854 17.903253393594802 14.513842046145337 7 28.483225371389825 32.61361171747947 20.799236679080256 18.103926232050448 8 26.172656555914227 35.04479370763695 19.932330153645044 18.850219582803778 9 26.66931184422668 16.992255453087825 20.12960383037503 36.20882887231047 10-11 24.874782358933057 26.89099735869617 27.27897587293315 20.955244409437626 12-13 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29.309015479294004 22.106418804497615 38-39 21.752099049062682 26.756769359212722 29.351937105503122 22.139194486221474 40-41 22.169734339768436 26.57253634153662 29.36473132557006 21.892997993124887 42-43 21.93516084708989 26.656050967199263 29.125403778355146 22.283384407355694 44-45 21.885952790317276 26.84667305265331 29.160353752581123 22.10702040444829 46-47 22.165380260635974 27.07321044023947 28.562617016080694 22.19879228304386 48-49 22.020295083483653 27.09991212247352 28.588316914111278 22.29147587993155 50-51 22.253689140558457 27.22748904598503 28.371398273258 22.147423540198517 52-53 22.366504800484265 27.19170560384572 28.29314552712085 22.14864406854917 54-55 22.07025851590624 27.251047964955475 28.424391303398664 22.25430221573962 56-57 22.02941685771245 27.52306239612117 28.28497617909414 22.16254456707224 58-59 22.107929201640637 26.963059714608683 28.43364087611767 22.495370207633005 60-61 21.967349352163517 27.278102546019383 28.48782159178661 22.266726510030484 62-63 21.982886680403002 27.251039382390235 28.636968899370174 22.12910503783659 64-65 21.78969424003262 27.135082495881036 28.547130487161 22.52809277692535 66-67 21.753019356514383 27.34604824384687 28.327544915275244 22.5733874843635 68-69 21.990089807420212 27.081791649153136 28.542235239792323 22.38588330363433 70-71 21.963881089806573 27.100802630113304 28.262690115352406 22.67262616472772 72-73 22.00874428966061 27.176201322189886 28.15550255612276 22.659551832026747 74-75 21.92030225712208 27.5110228746621 28.097367646868353 22.471307221347463 76-77 22.06685078866081 27.181083389206368 28.13810333624909 22.613962485883736 78-79 22.065787453358467 27.342251262119397 28.1009843513844 22.490976933137734 80-81 22.12420257027482 27.450933236049558 27.85592874802146 22.568935445654162 82-83 22.204339387104675 27.467849745706086 27.843537279051407 22.484273588137835 84-85 22.220211944895215 27.407874416618753 27.84446225328162 22.527451385204408 86-87 22.27657124589669 27.39527494028185 27.721652749698823 22.60650106412264 88-89 22.298263027662617 27.40433402983948 27.657622449259044 22.639780493238856 90-91 22.190980566472863 27.44621609347872 27.668024858274453 22.694778481773966 92-93 22.31805508989836 27.17122690001088 27.715657257554195 22.795060752536568 94-95 22.237075362415386 27.071994552203734 27.780911247552748 22.910018837828137 96-97 22.39523209769714 26.926819302409672 27.6883201864169 22.98962841347629 98-99 22.252546061500695 27.00281351776313 27.469858206549873 23.274782214186303 100-101 22.7917869183237 26.551714046838228 27.630265374153517 23.026233660684554 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1078.0 1 733.0 2 791.0 3 1046.5 4 705.5 5 577.0 6 948.5 7 1859.0 8 2692.5 9 2866.5 10 2355.0 11 1803.5 12 1727.5 13 1724.5 14 1791.5 15 1889.5 16 1931.0 17 2008.5 18 2130.0 19 2335.5 20 2585.5 21 2974.0 22 3481.0 23 4182.0 24 5003.0 25 6087.5 26 7783.5 27 9592.0 28 11173.5 29 13128.5 30 15455.5 31 18262.5 32 21659.5 33 23976.5 34 25651.5 35 27889.5 36 31028.5 37 34148.0 38 36253.5 39 37605.5 40 39330.0 41 42040.5 42 44314.5 43 46339.0 44 48185.5 45 50065.5 46 50954.5 47 51553.5 48 52110.5 49 51030.5 50 49541.0 51 47679.0 52 45548.5 53 44299.0 54 43576.0 55 40888.0 56 36871.0 57 33337.0 58 30529.0 59 27144.5 60 22348.0 61 18410.0 62 15225.0 63 11722.5 64 8918.5 65 7015.0 66 5312.0 67 4102.5 68 3179.0 69 2318.5 70 1747.5 71 1347.5 72 1074.5 73 831.5 74 634.5 75 507.5 76 363.5 77 220.5 78 166.0 79 112.5 80 65.0 81 42.5 82 25.5 83 19.5 84 13.0 85 6.0 86 5.5 87 3.5 88 2.0 89 3.5 90 3.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18468915099226021 2 0.050167966495493765 3 0.04898406168144081 4 0.05431163334467909 5 0.06193302058514496 6 0.06666863984135675 7 0.03855090050759919 8 0.03618309087949329 9 0.042472585204149586 10-11 0.04528435913752534 12-13 0.04069672798307016 14-15 0.03633107898124991 16-17 0.035073180116318645 18-19 0.043323516789250144 20-21 0.052757758276234586 22-23 0.049834993266541366 24-25 0.06962840187648912 26-27 0.055162564929779646 28-29 0.046912228256848154 30-31 0.055569532209610355 32-33 0.051795835614816564 34-35 0.05105589510603348 36-37 0.0485031003507318 38-39 0.02752578692673109 40-41 0.026230891036360678 42-43 0.023419117102984922 44-45 0.004698622230772646 46-47 0.011099107631746407 48-49 0.010914122504550634 50-51 0.009545232563301911 52-53 0.010211179021206695 54-55 0.011136104657185562 56-57 0.009323250410666983 58-59 0.011654063013333727 60-61 0.0114320808606988 62-63 0.00418066387462448 64-65 0.00662246755360869 66-67 0.004032675772867862 68-69 0.009175262308910364 70-71 0.025120980273186037 72-73 0.021643259881905495 74-75 0.021643259881905495 76-77 0.013466917259852308 78-79 0.004624628179894336 80-81 0.006437482426412917 82-83 0.005253577612359966 84-85 0.005253577612359966 86-87 0.008287333698370652 88-89 0.03189143592855134 90-91 0.038587897533038346 92-93 0.023900078433693933 94-95 0.03366729314963077 96-97 0.03769996892249863 98-99 0.02253118849244521 100-101 0.12479096680626878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1351460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.71664943220206 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14345633447373 54.454825656119375 2 9.499656521183496 12.29571881615529 3 2.6386029507242625 5.12284626458388 4 1.1220958962386398 2.904731469847545 5 0.618803215659126 2.002343538766549 6 0.39216573434537927 1.5227791409371196 7 0.27539765752777556 1.2475969559672284 8 0.18991154562022014 0.9832351136825148 9 0.1507089945764469 0.87780430514552 >10 0.8779395195331661 10.415505204308483 >50 0.061439281690892404 2.741633998062459 >100 0.027184255137347162 3.1730572342076635 >500 0.0019498950400757655 0.8665587795408415 >1k 5.734985411987546E-4 0.9263137750462703 >5k 1.1469970823975091E-4 0.46504974762920775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6265 0.4635727287526083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4906 0.36301481360898585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3600 0.26637858316191376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1372 0.10151983780504047 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.399405087830939E-5 0.0 14-15 0.0 0.0 0.0 1.8498512719577348E-4 0.0 16-17 0.0 0.0 0.0 2.2198215263492815E-4 0.0 18-19 0.0 0.0 0.0 2.2198215263492815E-4 0.0 20-21 0.0 0.0 0.0 2.589791780740828E-4 0.0 22-23 0.0 0.0 0.0 7.029434833439392E-4 0.0 24-25 0.0 0.0 0.0 0.0013688899412487236 0.0 26-27 0.0 0.0 0.0 0.002145827475470972 0.0 28-29 0.0 0.0 0.0 0.004365649001820253 0.0 30-31 0.0 0.0 0.0 0.011654063013333727 0.0 32-33 0.0 0.0 0.0 0.02537995945126012 0.0 34-35 0.0 0.0 0.0 0.047837153892827014 0.0 36-37 0.0 0.0 0.0 0.08457520015390763 0.0 38-39 0.0 0.0 0.0 0.14269752711881964 0.0 40-41 0.0 0.0 0.0 0.2038166131443032 0.0 42-43 0.0 0.0 0.0 0.26882038684089804 0.0 44-45 0.0 0.0 0.0 0.3475870540008583 0.0 46-47 0.0 0.0 0.0 0.44107853728560226 0.0 48-49 0.0 0.0 0.0 0.5469270270670238 0.0 50-51 0.0 0.0 0.0 0.6637636334038743 0.0 52-53 0.0 0.0 0.0 0.7769745312476877 0.0 54-55 0.0 0.0 0.0 0.8988797300697025 0.0 56-57 0.0 0.0 0.0 1.0472378020807127 0.0 58-59 0.0 0.0 0.0 1.2102096991401892 0.0 60-61 0.0 0.0 0.0 1.3847246681366818 0.0 62-63 0.0 0.0 0.0 1.5577967531410475 0.0 64-65 0.0 0.0 0.0 1.739896112352567 0.0 66-67 0.0 0.0 0.0 1.9499282257706483 0.0 68-69 0.0 3.6997025439154694E-5 0.0 2.1959214479155875 0.0 70-71 0.0 7.399405087830939E-5 0.0 2.460376185754665 0.0 72-73 0.0 7.399405087830939E-5 0.0 2.723425036627055 0.0 74-75 0.0 7.399405087830939E-5 0.0 2.9904695662468734 0.0 76-77 0.0 7.399405087830939E-5 0.0 3.2786393973924497 0.0 78-79 0.0 7.399405087830939E-5 0.0 3.599662587127995 3.6997025439154694E-5 80-81 0.0 7.399405087830939E-5 0.0 3.945510780933213 7.399405087830939E-5 82-83 0.0 7.399405087830939E-5 0.0 4.301126189454368 7.399405087830939E-5 84-85 0.0 1.1099107631746408E-4 0.0 4.6573705474079885 7.399405087830939E-5 86-87 0.0 1.4798810175661878E-4 0.0 5.033963269353144 7.399405087830939E-5 88-89 0.0 2.2198215263492815E-4 0.0 5.437600816894322 7.399405087830939E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3275 0.0 47.108624 1 GTATCAA 5355 0.0 40.19256 1 TATCAAC 6895 0.0 30.742989 2 ATCAACG 7020 0.0 30.195568 3 TGGTATC 1510 0.0 29.58669 2 TCAACGC 7190 0.0 29.481627 4 CAACGCA 7270 0.0 29.025385 5 GTGGTAT 1690 0.0 28.739561 1 AACGCAG 7520 0.0 28.053171 6 GTACATG 5755 0.0 25.236038 1 ACGCAGA 8395 0.0 25.069843 7 TACATGG 5745 0.0 24.487654 2 CGCAGAG 8600 0.0 24.417007 8 TAAGGTG 530 0.0 24.211254 5 GCAGAGT 9075 0.0 22.614637 9 ACATGGG 6140 0.0 22.602682 3 GGACCGA 460 0.0 21.690935 6 GAGTACT 5435 0.0 21.674864 12-13 GTATACG 135 3.7815803E-4 21.123358 4 GTAAGGT 570 0.0 20.845419 4 >>END_MODULE