FastQCFastQC Report
Thu 26 May 2016
SRR938354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351460
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61900.4580231749367351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.3794414929039705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.2871709114587187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14410.10662542731564383No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.10188980805943201No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.045.247911
GTATCAA52150.040.8625031
ATCAACG68950.030.7522323
TATCAAC69250.030.5662022
TCAACGC70800.030.0147154
CAACGCA71700.029.637965
AACGCAG74900.028.599136
TGGTATC14400.027.0864832
ATCACGC950.001220385125.0220243
ACGCAGA85850.024.8407177
CGCAGAG86900.024.3766048
GTGGTAT16300.024.2209841
GTACATG58900.023.904391
TACATGG59400.022.3418452
GCAGAGT94850.021.9829169
ACATGGG59350.021.7883433
ATACCGT1100.002878990521.5891446
AGTACTT57350.020.45604312-13
GAGTACT56050.020.42205812-13
AGAGTAC87200.020.18040310-11
Produced by FastQC (version 0.11.5)