##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1351460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806820031669453 31.0 31.0 34.0 30.0 34.0 2 32.02201175025528 31.0 31.0 34.0 30.0 34.0 3 32.08231838160212 33.0 31.0 34.0 30.0 34.0 4 35.7497558196321 37.0 35.0 37.0 33.0 37.0 5 35.626874639279 37.0 35.0 37.0 33.0 37.0 6 35.65974575644118 37.0 35.0 37.0 33.0 37.0 7 35.60381883296583 37.0 35.0 37.0 33.0 37.0 8 35.59413375164637 37.0 35.0 37.0 33.0 37.0 9 37.17510766134403 39.0 37.0 39.0 33.0 39.0 10-11 37.08662816509553 39.0 37.0 39.0 33.0 39.0 12-13 36.97455381587321 39.0 37.0 39.0 33.0 39.0 14-15 38.00821888920131 40.0 37.0 41.0 33.0 41.0 16-17 37.936699199384364 40.0 37.0 41.0 33.0 41.0 18-19 38.06028517307209 40.0 37.0 41.0 33.0 41.0 20-21 38.126290086277066 40.0 37.0 41.0 33.0 41.0 22-23 38.02030766726355 40.0 37.0 41.0 33.0 41.0 24-25 37.91365597205984 40.0 37.0 41.0 32.5 41.0 26-27 37.71762834268125 40.0 37.0 41.0 32.0 41.0 28-29 37.59002597191186 39.5 37.0 41.0 32.0 41.0 30-31 37.3433150074734 39.0 36.0 41.0 31.5 41.0 32-33 37.113873884539686 39.0 36.0 41.0 31.0 41.0 34-35 36.94472681396415 39.0 35.5 41.0 31.0 41.0 36-37 36.9231383096799 39.0 35.5 41.0 31.0 41.0 38-39 36.793765261272995 39.0 35.0 41.0 30.0 41.0 40-41 36.61750995219984 39.0 35.0 41.0 30.0 41.0 42-43 36.38935373595963 39.0 35.0 40.5 30.0 41.0 44-45 36.106987258224436 39.0 35.0 40.0 29.5 41.0 46-47 35.70711156822991 38.0 35.0 40.0 28.5 41.0 48-49 35.336753954982015 38.0 34.5 40.0 27.0 41.0 50-51 34.97147270359463 38.0 34.0 40.0 26.0 41.0 52-53 34.48908106788214 38.0 33.0 40.0 24.0 41.0 54-55 34.182566631642814 38.0 33.0 40.0 24.0 41.0 56-57 33.851747739481745 37.0 33.0 40.0 23.0 41.0 58-59 33.41904902845811 37.0 32.0 40.0 22.0 41.0 60-61 33.05966806268776 36.0 32.0 39.0 21.5 40.5 62-63 32.60036516064109 36.0 31.0 39.0 19.5 40.0 64-65 32.10937097657349 35.0 31.0 39.0 18.0 40.0 66-67 31.619353513977476 35.0 30.0 38.0 16.0 40.0 68-69 31.0861668121883 35.0 29.5 38.0 13.0 40.0 70-71 30.505051943823716 34.0 29.0 37.0 8.0 39.0 72-73 30.063629704171785 34.0 29.0 36.5 7.5 39.0 74-75 29.531076391458125 34.0 28.0 36.0 7.0 39.0 76-77 28.259958489337457 31.5 26.5 34.5 3.5 37.0 78-79 28.95572713953798 33.5 28.0 35.0 2.0 37.0 80-81 28.66312136504225 33.5 27.5 35.0 2.0 37.0 82-83 28.18623599662587 33.0 26.5 35.0 2.0 36.5 84-85 27.652609030233968 33.0 26.0 35.0 2.0 36.0 86-87 27.07120151539816 32.5 25.5 35.0 2.0 35.5 88-89 26.42836894913649 32.0 24.5 34.0 2.0 35.0 90-91 25.877424045106775 32.0 23.5 34.0 2.0 35.0 92-93 25.121792357894428 31.0 20.0 34.0 2.0 35.0 94-95 24.35255020496352 31.0 16.5 34.0 2.0 35.0 96-97 23.35553586491646 31.0 2.0 34.0 2.0 35.0 98-99 22.11692687907892 30.0 2.0 34.0 2.0 35.0 100-101 18.447366921699498 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2976309440107556 1101 2 0.32662755872114957 1101 3 0.3045824880491921 1101 4 0.13141152509893317 1101 5 0.20919453532309262 1101 6 0.13929827924552995 1101 7 0.3153238841324537 1101 8 0.1645915365888868 1101 9 0.22911270440084763 1101 10-11 0.31976641657725224 1101 12-13 0.30882696594568415 1101 14-15 0.39231118301395185 1101 16-17 0.3148377123200845 1101 18-19 0.4515747319472112 1101 20-21 0.42029826566520967 1101 22-23 0.4130687431852067 1101 24-25 0.4715913160989018 1101 26-27 0.4840955823268942 1101 28-29 0.5029608041695113 1101 30-31 0.41831761911348053 1101 32-33 0.3342397859427777 1101 34-35 0.19377980207342915 1101 36-37 0.3704359504797452 1101 38-39 0.4039508812785968 1101 40-41 0.453367512415646 1101 42-43 0.4581560739630106 1101 44-45 0.41876553284132 1101 46-47 0.6269752231408674 1101 48-49 0.6831701406745836 1101 50-51 0.4115417490229518 1101 52-53 0.3634181099463589 1101 54-55 0.388869882875035 1101 56-57 0.41107633374458175 1101 58-59 0.4103461584786743 1101 60-61 0.35181738919586536 1101 62-63 0.3126470741343397 1101 64-65 0.3997453838877547 1101 66-67 0.2890289170958589 1101 68-69 0.3485072333240673 1101 70-71 0.19603167628550722 1101 72-73 0.2754991772208868 1101 74-75 0.2004449799832031 1101 76-77 0.10299893520173597 1101 78-79 0.15182882726908176 1101 80-81 0.30369299183768916 1101 82-83 0.4207281970509449 1101 84-85 0.5518781995508597 1101 86-87 0.3368569352942572 1101 88-89 0.41235538734505184 1101 90-91 0.388580318722866 1101 92-93 0.41514037936913795 1101 94-95 0.29112190263938587 1101 96-97 0.5278847692196464 1101 98-99 0.5181217245261784 1101 100-101 0.30466297142590903 1102 1 0.28960955870557115 1102 2 0.22477056347027968 1102 3 0.22334423843389573 1102 4 0.2824136453028032 1102 5 0.24152590644092697 1102 6 0.2637014781939584 1102 7 0.2399116134920689 1102 8 0.23079118964643186 1102 9 0.3339146027890507 1102 10-11 0.4047107572760069 1102 12-13 0.4330257279488521 1102 14-15 0.4597245026970924 1102 16-17 0.40164157608355566 1102 18-19 0.3706502472262727 1102 20-21 0.3145964655457689 1102 22-23 0.30669991618562875 1102 24-25 0.42009469231027197 1102 26-27 0.36287142527381633 1102 28-29 0.4164593301380606 1102 30-31 0.28567501091515624 1102 32-33 0.2628745452790966 1102 34-35 0.2536333784560654 1102 36-37 0.4546804027182816 1102 38-39 0.4594989587181786 1102 40-41 0.39592768228473574 1102 42-43 0.3863683724093576 1102 44-45 0.5719962369322644 1102 46-47 0.4222140857510297 1102 48-49 0.4619418759240048 1102 50-51 0.4645064007060071 1102 52-53 0.3882494573659372 1102 54-55 0.24043444166093053 1102 56-57 0.4967145593456408 1102 58-59 0.6252675276690525 1102 60-61 0.5649149406921694 1102 62-63 0.6226411021741143 1102 64-65 0.5085001511866878 1102 66-67 0.5271328671209794 1102 68-69 0.4332442932869718 1102 70-71 0.21635435172368744 1102 72-73 0.28996269612735404 1102 74-75 0.179770938419626 1102 76-77 0.23032270707917846 1102 78-79 0.21666598866051956 1102 80-81 0.32478823038432125 1102 82-83 0.2868535955765985 1102 84-85 0.18222713265542723 1102 86-87 0.1283625328220559 1102 88-89 0.23262245087849465 1102 90-91 0.011696544443999812 1102 92-93 0.07778782201145873 1102 94-95 -0.17639948108591597 1102 96-97 -0.1491054892177175 1102 98-99 -0.030224611249654032 1102 100-101 0.2804218685407278 1103 1 0.24074091266511033 1103 2 0.2198711393367816 1103 3 0.21411902252170023 1103 4 0.1540685384169791 1103 5 0.12717682518862006 1103 6 0.1112197332888698 1103 7 0.14479310406668588 1103 8 0.06228638351303317 1103 9 0.1952791352816945 1103 10-11 0.23227385230635633 1103 12-13 0.26542665711249924 1103 14-15 0.3991172252951216 1103 16-17 0.3226010758278406 1103 18-19 0.18772824854622883 1103 20-21 0.3872937996044925 1103 22-23 0.3623485947480205 1103 24-25 0.32706601612694897 1103 26-27 0.5382486489294465 1103 28-29 0.5866518230416204 1103 30-31 0.5797159368664637 1103 32-33 0.5336936258611615 1103 34-35 0.4395524552340362 1103 36-37 0.5253250386821264 1103 38-39 0.4914086170567131 1103 40-41 0.3447026149131247 1103 42-43 0.46488475383985417 1103 44-45 0.44417615460696425 1103 46-47 0.3601772561385488 1103 48-49 0.43892593437895044 1103 50-51 0.4051496418374967 1103 52-53 0.38908424339804526 1103 54-55 0.31226076027565597 1103 56-57 0.38530972163227517 1103 58-59 0.3694220630177796 1103 60-61 0.14997918449679304 1103 62-63 0.24222201241393293 1103 64-65 0.335107091655118 1103 66-67 0.24163966707967788 1103 68-69 -0.049283469009441916 1103 70-71 -0.16325319921033454 1103 72-73 0.17806785561828065 1103 74-75 0.22529742121397334 1103 76-77 -0.04151804291795358 1103 78-79 0.027490491577889742 1103 80-81 -0.0016940205248836548 1103 82-83 -0.02566674572861416 1103 84-85 -0.27927881530827037 1103 86-87 -0.19472079542909526 1103 88-89 -0.06437147405295462 1103 90-91 0.0655648493241614 1103 92-93 0.15500235990833033 1103 94-95 2.3278977965546233E-4 1103 96-97 0.14257961922596962 1103 98-99 0.2227089144215988 1103 100-101 -0.16611469025689374 1104 1 0.24756220056851674 1104 2 0.24205141631367866 1104 3 0.21350828131233612 1104 4 0.17178925222364683 1104 5 0.1316392692136148 1104 6 0.2092085509630266 1104 7 0.19910585887829058 1104 8 0.23557094973697446 1104 9 0.264302110674592 1104 10-11 0.30568667418426543 1104 12-13 0.18452073200089814 1104 14-15 0.3200907698296831 1104 16-17 0.34800099608577995 1104 18-19 0.4022594528126575 1104 20-21 0.27952707278558364 1104 22-23 0.4610172941458117 1104 24-25 0.3668805812676865 1104 26-27 0.43405216541600566 1104 28-29 0.45928520085535496 1104 30-31 0.2223363969274459 1104 32-33 0.2980780217689656 1104 34-35 0.3434514616848503 1104 36-37 0.45075180850643903 1104 38-39 0.481230269100152 1104 40-41 0.3708708591358558 1104 42-43 0.48173055205556636 1104 44-45 0.41400957206126066 1104 46-47 0.4704762542924996 1104 48-49 0.6203883830133137 1104 50-51 0.4789266037572091 1104 52-53 0.4106511863963007 1104 54-55 0.3019835990007991 1104 56-57 0.40986699187258324 1104 58-59 0.526910614500963 1104 60-61 0.5372582517451647 1104 62-63 0.5899400297405464 1104 64-65 0.6913478297070625 1104 66-67 0.7489596309422346 1104 68-69 0.6383105908985094 1104 70-71 0.42878521295470406 1104 72-73 0.14248152101045974 1104 74-75 0.34483243973210875 1104 76-77 0.049914525490823536 1104 78-79 0.22951705766815778 1104 80-81 0.23189951161047517 1104 82-83 0.06057135346036446 1104 84-85 -0.09076172953485084 1104 86-87 0.27419276509996493 1104 88-89 -0.12425320920330307 1104 90-91 0.0981646018011233 1104 92-93 0.1539243440567155 1104 94-95 0.3122985009244168 1104 96-97 0.485220418121564 1104 98-99 0.2809713211107159 1104 100-101 -0.21758548184663695 1105 1 0.1740194353410871 1105 2 0.2673077741834291 1105 3 0.26945623790824413 1105 4 0.1941852983777963 1105 5 0.15136147522091647 1105 6 0.2028960829874933 1105 7 0.22598857312600984 1105 8 0.1840334511152406 1105 9 0.08167023473817636 1105 10-11 0.26191774326630224 1105 12-13 0.28950908968903377 1105 14-15 0.4121720519757588 1105 16-17 0.3238699535962013 1105 18-19 0.3367682056259511 1105 20-21 0.2824579193989365 1105 22-23 0.2742846651885529 1105 24-25 0.29960488224252657 1105 26-27 0.32939297058402417 1105 28-29 0.40286539011589184 1105 30-31 0.48023873771315095 1105 32-33 0.3624407105916987 1105 34-35 0.3588305016319211 1105 36-37 0.34207455674064136 1105 38-39 0.5537717630779397 1105 40-41 0.49701076794315924 1105 42-43 0.34119665963181944 1105 44-45 0.4161321049613278 1105 46-47 0.22111593284794395 1105 48-49 0.2506209373819317 1105 50-51 0.40206626609772655 1105 52-53 0.4496099615861837 1105 54-55 0.6070332490828534 1105 56-57 0.5493842088736045 1105 58-59 0.599186183806772 1105 60-61 0.5270947405017381 1105 62-63 0.5650241851702731 1105 64-65 0.2739141973706225 1105 66-67 0.37131659914153303 1105 68-69 0.2503435201560613 1105 70-71 0.4030853770496883 1105 72-73 0.7277938852105166 1105 74-75 0.5953099690973254 1105 76-77 0.7393534244329594 1105 78-79 0.7829346761505711 1105 80-81 0.7406685398179462 1105 82-83 0.38203779830694273 1105 84-85 0.5873210508697433 1105 86-87 0.5735939481404593 1105 88-89 0.3176430607167262 1105 90-91 0.36488380480542304 1105 92-93 0.18704007317676385 1105 94-95 0.17835029786174417 1105 96-97 -0.06602521195776134 1105 98-99 -0.03525202966987351 1105 100-101 -0.06701293305594547 1106 1 0.21770234070865513 1106 2 0.2620313962936578 1106 3 0.19613090393495725 1106 4 0.18534280577720352 1106 5 0.09551314308748715 1106 6 0.17904839080286905 1106 7 0.18990665967908882 1106 8 0.2466620408280633 1106 9 0.11459240096488088 1106 10-11 0.25010108859867586 1106 12-13 0.28670291418307414 1106 14-15 0.40143211117102595 1106 16-17 0.1900630581478353 1106 18-19 0.43458678013998764 1106 20-21 0.27886140711991914 1106 22-23 0.20178806491657753 1106 24-25 0.3332869876740858 1106 26-27 0.15889701026085135 1106 28-29 0.32454045611061133 1106 30-31 0.28601507560611594 1106 32-33 0.28774268643362433 1106 34-35 0.13907208230546786 1106 36-37 -0.12253147477683513 1106 38-39 0.09335739622728312 1106 40-41 0.24550048876549369 1106 42-43 0.2279017232267364 1106 44-45 0.20762318567487625 1106 46-47 -0.026996218179967002 1106 48-49 0.021104098729026077 1106 50-51 0.16366133849194142 1106 52-53 0.04406960481472311 1106 54-55 -0.08500338798618401 1106 56-57 -0.06996784296224945 1106 58-59 -0.04461591202556292 1106 60-61 -0.5030420485551375 1106 62-63 -0.18731722751670787 1106 64-65 -0.6133068249475926 1106 66-67 -0.2331174461348411 1106 68-69 -0.9527154351275158 1106 70-71 -0.8494480202785262 1106 72-73 -0.6714173778884387 1106 74-75 -0.6768642569645884 1106 76-77 -0.5796650540887569 1106 78-79 -0.5515089683578687 1106 80-81 -1.1698322201212576 1106 82-83 -0.7209451630561503 1106 84-85 -0.6840700378431599 1106 86-87 -0.4719784060712051 1106 88-89 -0.384048003998096 1106 90-91 -0.37948304584652703 1106 92-93 -0.38054012040774765 1106 94-95 -0.393367164741246 1106 96-97 -0.453047850146703 1106 98-99 -0.7448144646750698 1106 100-101 -0.6916645609257195 1107 1 0.18801680611746008 1107 2 0.2800327939176981 1107 3 0.21907989065753242 1107 4 0.1676908141629454 1107 5 0.09129231848930175 1107 6 0.15299667871342137 1107 7 0.10944544374617493 1107 8 0.09605407437103963 1107 9 0.15207667769443844 1107 10-11 0.1409606273827393 1107 12-13 0.17977069894897824 1107 14-15 0.3885460175302171 1107 16-17 0.3527604725433662 1107 18-19 0.22687888356416153 1107 20-21 0.3573100444364741 1107 22-23 0.22986633186277317 1107 24-25 0.3442164076601131 1107 26-27 0.31575934429299224 1107 28-29 0.3142958719037665 1107 30-31 0.17049446065153973 1107 32-33 0.19046805330994232 1107 34-35 0.16122442332573428 1107 36-37 0.19454749098137114 1107 38-39 0.25816522292189603 1107 40-41 0.20219510791293516 1107 42-43 0.25187097549788007 1107 44-45 0.19586217938556416 1107 46-47 0.17157121717991686 1107 48-49 0.2788888646269996 1107 50-51 0.42837133150381845 1107 52-53 0.37912201571618453 1107 54-55 0.5740811682681866 1107 56-57 0.31795668533963806 1107 58-59 0.14355320048316855 1107 60-61 -0.05304903667533267 1107 62-63 0.31626069002346924 1107 64-65 0.10936962508734638 1107 66-67 0.17773510452903096 1107 68-69 0.28812313929596556 1107 70-71 -0.011209026567836844 1107 72-73 0.010441462083612407 1107 74-75 -0.11854839393174288 1107 76-77 0.055590012298385005 1107 78-79 0.15798089886784794 1107 80-81 0.15774989029103992 1107 82-83 0.04361807943162077 1107 84-85 0.11848761414845299 1107 86-87 0.25317882663319935 1107 88-89 0.02233913786074382 1107 90-91 0.04660360684390241 1107 92-93 0.2032614169786946 1107 94-95 0.14558461722939953 1107 96-97 0.29277325397628573 1107 98-99 0.23522746404610118 1107 100-101 0.14332495689398783 1108 1 0.16975362275994144 1108 2 0.2583561826184422 1108 3 0.21647278427683858 1108 4 0.12135420178859846 1108 5 -0.025825888251546303 1108 6 0.0027520945065688807 1108 7 0.026259936032367648 1108 8 0.08449679866281912 1108 9 0.15858100495348282 1108 10-11 0.18306405156163663 1108 12-13 0.1476858201659823 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-0.8239012881636079 2316 68-69 -0.6878520060692317 2316 70-71 -0.7162219389874913 2316 72-73 -0.7269919781991661 2316 74-75 -0.2855020557918344 2316 76-77 -0.34939141102756466 2316 78-79 -0.5472168819548209 2316 80-81 -0.6101665056923515 2316 82-83 -0.5327569206865839 2316 84-85 -0.6096336122429982 2316 86-87 -0.3735796690391773 2316 88-89 -0.5518413182866766 2316 90-91 -0.624860182116759 2316 92-93 -0.5184913056931713 2316 94-95 -0.44723776064161314 2316 96-97 -0.4143689547425069 2316 98-99 -0.35526301877721167 2316 100-101 -0.45683471077787274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 21.0 5 56.0 6 119.0 7 309.0 8 621.0 9 1122.0 10 1920.0 11 2944.0 12 4054.0 13 5824.0 14 7458.0 15 9642.0 16 12140.0 17 15009.0 18 14459.0 19 12066.0 20 11819.0 21 12162.0 22 13478.0 23 14844.0 24 17309.0 25 20333.0 26 24163.0 27 29048.0 28 34630.0 29 41425.0 30 48963.0 31 57916.0 32 70251.0 33 86065.0 34 109202.0 35 143701.0 36 187092.0 37 219704.0 38 116122.0 39 5460.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55915670023301 19.514256137653327 15.373379614385405 22.55320754772826 2 15.849002686597883 21.024238606019193 39.7167856473931 23.409973059989827 3 19.30959926656391 25.06084219358954 29.911198885342895 25.718359654503654 4 13.181226229411156 17.531632456750476 36.57407544433428 32.71306586950409 5 13.555856242172707 37.64277985518162 34.081346839050454 14.72001706359522 6 31.65709676941974 36.515842126293045 17.451422905598392 14.375638198688826 7 28.387743625412515 32.7546505260977 20.662912701818772 18.194693146671007 8 26.26929394876652 35.37263404022317 19.610643304278337 18.747428706731977 9 26.35949269678718 16.89683749426546 20.135039142852914 36.60863066609444 10-11 24.87143533659894 27.062325189054796 27.206502597191186 20.85973687715508 12-13 25.868468175158714 24.329317922839003 27.75860920781969 22.043604694182587 14-15 22.520385361016974 25.701870569606207 26.361157562931943 25.416586506444883 16-17 21.889216107024996 28.943808917763015 27.006459680641676 22.160515294570317 18-19 21.77567223595223 27.170985452769596 29.685599277818064 21.36774303346011 20-21 22.46148609651784 26.258971778668993 29.919642460746154 21.359899664067008 22-23 22.24042886951889 26.27232770485253 29.768879582081603 21.718363843546978 24-25 22.133992374898952 26.464280230787875 29.82816699748235 21.57356039683083 26-27 21.687932319907944 26.83386890589648 29.670313000075847 21.80788577411973 28-29 21.75861660722478 26.973606322051708 29.695810456839272 21.571966613884243 30-31 21.828319003152146 26.585914492474807 29.72962573809066 21.85614076628239 32-33 21.53667145161529 26.712296331374958 29.572721353203203 22.17831086380655 34-35 22.219167600320276 26.81474141618356 29.372569972219797 21.593521011276366 36-37 22.309843543221238 26.416514886313646 29.321115431132043 21.95252613933307 38-39 21.779038847958372 26.794580029221343 29.405150696365535 22.02123042645475 40-41 22.172767207847585 26.561375584441727 29.44676118370885 21.819096024001837 42-43 21.92938578836455 26.593651587621054 29.237133876352615 22.23982874766178 44-45 21.92188448048777 26.878486969647643 29.230609858967338 21.96901869089725 46-47 22.178976810264455 27.099396208544835 28.605508117147384 22.11611886404333 48-49 22.035465348585973 27.068503692303135 28.616718215855446 22.279312743255442 50-51 22.249789116954997 27.27590901691504 28.419487073239313 22.05481479289065 52-53 22.477135838278603 27.23129060423542 28.23979252068134 22.05178103680464 54-55 22.05836650733281 27.259149364391106 28.429143296878927 22.253340831397157 56-57 22.037056220679858 27.445096414248294 28.340165450697764 22.177681914374084 58-59 22.13546830834801 27.037870155239517 28.364842466665678 22.46181906974679 60-61 22.017447797197107 27.249826113980436 28.512867565447735 22.21985852337472 62-63 21.913079188433247 27.326113980435974 28.55212140943868 22.208685421692095 64-65 21.761465378183594 27.21427197253341 28.41549139449188 22.608771254791115 66-67 21.698940405191422 27.40421470113803 28.249670726473592 22.647174167196958 68-69 21.942780484141228 27.133966655678428 28.433761060850077 22.48949179933027 70-71 21.870696780294484 27.118765719122816 28.22978721515044 22.780750285432262 72-73 21.90431089340417 27.241427789205748 28.115334527104018 22.738926790286058 74-75 21.78259067970935 27.45663948618531 27.98495700945644 22.7758128246489 76-77 21.7918029390437 27.199547156408627 28.275753629408197 22.732896275139478 78-79 21.84744646530419 27.25667056368668 28.24105041954627 22.65483255146286 80-81 21.819424938472945 27.37199190787887 28.072689979118536 22.73589317452965 82-83 21.902512116615487 27.388101668578198 27.97724666099375 22.732139553812573 84-85 21.89157440038479 27.30304964342204 27.969216823449973 22.8361591327432 86-87 21.867839747230246 27.330359934217707 27.866348731248998 22.935451587303053 88-89 21.74001514658782 27.374257238193227 27.78769915043699 23.098028464781965 90-91 21.65807195875724 27.296947713245324 27.943493217839404 23.101487110158033 92-93 21.712058053223597 27.02550113785236 27.982991595320435 23.279449213603616 94-95 21.504336051381472 26.967168839625295 28.029020466754474 23.499474642238763 96-97 21.645442706406403 26.7568407500037 27.972969973214152 23.62474657037574 98-99 21.42350547685669 26.849158261864304 27.80364487430071 23.923691386978298 100-101 21.42164769952496 26.60082429372678 28.168610243736403 23.808917763011852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1084.0 1 737.0 2 771.5 3 1032.0 4 685.0 5 559.0 6 960.0 7 1900.5 8 2744.5 9 2889.5 10 2351.0 11 1729.5 12 1602.5 13 1677.0 14 1789.0 15 1845.5 16 1898.0 17 2008.5 18 2130.0 19 2255.5 20 2474.5 21 2849.5 22 3403.5 23 4171.0 24 4934.0 25 5887.0 26 7456.5 27 9294.5 28 10947.0 29 12981.0 30 15375.0 31 18163.0 32 21753.0 33 24140.0 34 25750.5 35 28120.0 36 30968.0 37 33995.0 38 36341.0 39 37851.5 40 39602.5 41 42398.0 42 44983.5 43 46908.5 44 48769.0 45 50684.5 46 51180.5 47 51836.0 48 52667.5 49 51730.5 50 50274.5 51 48231.5 52 46107.5 53 45138.0 54 44567.0 55 41404.5 56 36960.0 57 33178.5 58 30281.0 59 26837.0 60 21726.5 61 17567.5 62 14656.5 63 11236.0 64 8181.0 65 6197.5 66 4526.5 67 3525.5 68 2766.5 69 2070.0 70 1549.0 71 1146.0 72 942.5 73 784.5 74 601.5 75 455.0 76 322.0 77 196.5 78 121.0 79 80.0 80 45.0 81 23.5 82 13.5 83 7.5 84 4.0 85 4.5 86 3.0 87 2.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.179583561481658E-4 2 0.05038994864812869 3 9.61922661418022E-4 4 0.0 5 0.09005075991890252 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8498512719577348E-4 26-27 0.007584390215026712 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010211179021206695 36-37 3.699702543915469E-5 38-39 0.003736699569354624 40-41 0.004698622230772646 42-43 2.959762035132375E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0011469077886137955 70-71 7.399405087830937E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0016278691193228065 82-83 7.399405087830939E-4 84-85 0.00536456868867743 86-87 0.002404806653545055 88-89 3.699702543915469E-5 90-91 0.003477720391280541 92-93 7.769375342222486E-4 94-95 0.0 96-97 0.0 98-99 3.699702543915469E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1351460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.199958502625286 #Duplication Level Percentage of deduplicated Percentage of total 1 82.48082872795418 51.303041241408934 2 10.465946641533398 13.019628935881359 3 2.9277634752270334 5.463202999938704 4 1.2701740836700492 3.1601910118154857 5 0.6917343717092459 2.1512924607577335 6 0.4226253241693431 1.5772366575295023 7 0.29358412666361877 1.2782644346854604 8 0.21328450526698658 1.0613029901487623 9 0.1664963043922978 0.9320456901637252 >10 0.9650732202659733 11.209295720918197 >50 0.06831352073367516 2.923539459800991 >100 0.03107967894396175 3.467661583263565 >500 0.002262475766695047 0.9517514734741633 >1k 5.953883596565914E-4 0.6632633809308275 >5k 2.3815534386263657E-4 0.8382819592825355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6190 0.4580231749367351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5128 0.3794414929039705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3881 0.2871709114587187 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1441 0.10662542731564383 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1377 0.10188980805943201 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.6997025439154694E-5 0.0 14-15 0.0 0.0 0.0 1.1099107631746408E-4 0.0 16-17 0.0 0.0 0.0 1.4798810175661878E-4 0.0 18-19 0.0 0.0 0.0 1.4798810175661878E-4 0.0 20-21 0.0 0.0 0.0 1.8498512719577348E-4 0.0 22-23 0.0 0.0 0.0 6.659464579047845E-4 0.0 24-25 0.0 0.0 0.0 0.001331892915809569 0.0 26-27 0.0 0.0 0.0 0.0020718334245926626 0.0 28-29 0.0 0.0 0.0 0.004365649001820253 0.0 30-31 0.0 0.0 0.0 0.011802051115090346 0.0 32-33 0.0 0.0 0.0 0.025712932680212512 0.0 34-35 0.0 0.0 0.0 0.04831811522353603 0.0 36-37 0.0 0.0 0.0 0.08572210794252141 0.0 38-39 0.0 0.0 0.0 0.1438074378819943 0.0 40-41 0.0 0.0 0.0 0.2052594971364302 0.0 42-43 0.0 0.0 0.0 0.2708182262146124 0.0 44-45 0.0 0.0 0.0 0.35017684578159913 0.0 46-47 0.0 0.0 0.0 0.44466724875320024 0.0 48-49 0.0 0.0 0.0 0.5515146582214789 0.0 50-51 0.0 0.0 0.0 0.668943216965356 0.0 52-53 0.0 0.0 0.0 0.7826720731653175 0.0 54-55 0.0 0.0 0.0 0.9043552898346973 0.0 56-57 0.0 0.0 0.0 1.0524913796930728 0.0 58-59 0.0 0.0 0.0 1.215241294599914 0.0 60-61 0.0 0.0 0.0 1.3896822695455286 0.0 62-63 0.0 0.0 0.0 1.5638272682876297 0.0 64-65 0.0 0.0 0.0 1.7456306512956359 0.0 66-67 0.0 0.0 0.0 1.9565136962988174 0.0 68-69 0.0 0.0 0.0 2.2022849362911225 0.0 70-71 0.0 0.0 0.0 2.4695514480635756 0.0 72-73 0.0 0.0 0.0 2.7359670282509287 0.0 74-75 0.0 0.0 0.0 3.0061933020585148 0.0 76-77 0.0 0.0 0.0 3.29876577923135 0.0 78-79 0.0 0.0 0.0 3.625079543604694 0.0 80-81 0.0 0.0 0.0 3.9781421573705473 0.0 82-83 0.0 0.0 0.0 4.338752164325988 0.0 84-85 0.0 0.0 0.0 4.704060793512202 0.0 86-87 0.0 0.0 0.0 5.091123673656638 0.0 88-89 0.0 0.0 0.0 5.507599189025202 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3385 0.0 45.24791 1 GTATCAA 5215 0.0 40.862503 1 ATCAACG 6895 0.0 30.752232 3 TATCAAC 6925 0.0 30.566202 2 TCAACGC 7080 0.0 30.014715 4 CAACGCA 7170 0.0 29.63796 5 AACGCAG 7490 0.0 28.59913 6 TGGTATC 1440 0.0 27.086483 2 ATCACGC 95 0.0012203851 25.022024 3 ACGCAGA 8585 0.0 24.840717 7 CGCAGAG 8690 0.0 24.376604 8 GTGGTAT 1630 0.0 24.220984 1 GTACATG 5890 0.0 23.90439 1 TACATGG 5940 0.0 22.341845 2 GCAGAGT 9485 0.0 21.982916 9 ACATGGG 5935 0.0 21.788343 3 ATACCGT 110 0.0028789905 21.589144 6 AGTACTT 5735 0.0 20.456043 12-13 GAGTACT 5605 0.0 20.422058 12-13 AGAGTAC 8720 0.0 20.180403 10-11 >>END_MODULE