FastQCFastQC Report
Thu 26 May 2016
SRR938353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348536
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.467618217088754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.36261545854170746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36270.26895833704105787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30200.047.651121
GTATCAA52750.044.8056031
ATCAACG67600.034.9450263
TATCAAC68700.034.593062
TCAACGC68700.034.3163154
CAACGCA69950.033.5671845
AACGCAG72500.032.249446
GTACATG59100.028.784181
TACATGG58400.028.5673482
ACGCAGA84450.027.5110787
CGCAGAG84350.027.4873668
ACATGGG60750.027.0710743
GCAGAGT90500.025.0410259
TGGTATC14100.024.9452762
GTAAGGT6100.024.934224
GTGGTAT14950.024.2239821
CGGTTCG601.7118617E-423.74956330-31
TAAGGTG6900.023.4210035
CATGGGA29000.023.2737314
GAGTACT54600.022.44422712-13
Produced by FastQC (version 0.11.5)