FastQCFastQC Report
Thu 26 May 2016
SRR938353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348536
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.45508610819436784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53650.39783884152888765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.28171290940694205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14050.10418705915155398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.048.290281
GTATCAA52250.040.3242951
TATCAAC68400.030.5251432
ATCAACG68400.030.4443053
TCAACGC69800.029.8336774
CAACGCA70800.029.4794485
AACGCAG72500.028.7593866
GTACATG59350.025.7237131
TACATGG60050.024.7902362
ACGCAGA85150.024.3752967
TGGTATC15450.024.3191222
CGCAGAG85350.024.3181788
ACATGGG60400.023.3780143
GTGGTAT17250.023.1600511
GCAGAGT93900.021.8004159
CATGGGG29350.021.706254
CCATGCG1100.002879180221.5888969
GAGTACT56200.020.36731512-13
AGAGTAC85450.019.62075610-11
CAGAGTA90600.019.39664510-11
Produced by FastQC (version 0.11.5)