##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1348536 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816845823915713 31.0 31.0 34.0 30.0 34.0 2 32.03890070417104 31.0 31.0 34.0 30.0 34.0 3 32.099804528763045 33.0 31.0 34.0 30.0 34.0 4 35.769133341638636 37.0 35.0 37.0 35.0 37.0 5 35.643178231801 37.0 35.0 37.0 33.0 37.0 6 35.68906354743218 37.0 35.0 37.0 33.0 37.0 7 35.632324239026616 37.0 35.0 37.0 33.0 37.0 8 35.616580499148704 37.0 35.0 37.0 33.0 37.0 9 37.19442491709528 39.0 37.0 39.0 33.0 39.0 10-11 37.121245558146015 39.0 37.0 39.0 33.0 39.0 12-13 37.008883707961814 39.0 37.0 39.0 33.0 39.0 14-15 38.04304297401033 40.0 37.0 41.0 33.0 41.0 16-17 37.964875242485185 40.0 37.0 41.0 33.0 41.0 18-19 37.97698986159806 40.0 37.0 41.0 33.0 41.0 20-21 37.981376470483546 40.0 37.0 41.0 33.0 41.0 22-23 38.007235253638015 40.0 37.0 41.0 33.0 41.0 24-25 37.920193083462365 40.0 37.0 41.0 33.0 41.0 26-27 37.741544534220814 40.0 37.0 41.0 32.0 41.0 28-29 37.60080746824705 39.5 37.0 41.0 32.0 41.0 30-31 37.34475312487022 39.0 36.0 41.0 31.5 41.0 32-33 37.101975401472416 39.0 36.0 41.0 31.0 41.0 34-35 36.91995171059578 39.0 35.5 40.5 31.0 41.0 36-37 36.89990923490363 39.0 35.5 41.0 31.0 41.0 38-39 36.751247649302655 39.0 35.0 41.0 30.0 41.0 40-41 36.57007710583922 39.0 35.0 40.5 30.0 41.0 42-43 36.33324064022021 39.0 35.0 40.0 30.0 41.0 44-45 36.026608485053416 38.5 35.0 40.0 29.5 41.0 46-47 35.624460896854075 38.0 35.0 40.0 27.5 41.0 48-49 35.21555486839061 38.0 34.0 40.0 26.5 41.0 50-51 34.835035178890294 38.0 34.0 40.0 26.0 41.0 52-53 34.327617505205644 38.0 33.0 40.0 24.0 41.0 54-55 33.998590693908056 37.5 33.0 40.0 23.5 41.0 56-57 33.63468086873469 37.0 33.0 40.0 23.0 41.0 58-59 33.18035781024756 36.5 32.0 39.5 21.5 41.0 60-61 32.764219865098156 36.0 31.0 39.0 20.0 40.5 62-63 32.29244751345163 35.5 31.0 39.0 18.5 40.0 64-65 31.787268563835152 35.0 30.5 38.5 16.0 40.0 66-67 31.288028276590317 35.0 29.5 38.0 12.5 40.0 68-69 30.718379042161274 34.5 29.0 38.0 8.0 40.0 70-71 30.15123734182847 34.0 28.5 37.0 7.0 39.0 72-73 29.56015931350739 34.0 28.0 36.5 4.0 39.0 74-75 28.931304763091234 33.5 27.0 36.0 2.0 39.0 76-77 27.64232953365724 31.5 26.0 34.5 2.0 36.5 78-79 28.25474662893686 33.0 26.0 35.0 2.0 37.0 80-81 27.928487633997165 33.0 26.0 35.0 2.0 37.0 82-83 27.41164863229458 33.0 26.0 35.0 2.0 36.0 84-85 26.804960342178482 32.0 25.0 35.0 2.0 36.0 86-87 26.14052387181358 32.0 23.5 35.0 2.0 35.5 88-89 25.384556659963103 31.0 20.5 34.0 2.0 35.0 90-91 24.82456901410122 31.0 19.0 34.0 2.0 35.0 92-93 24.026884339758077 31.0 11.0 34.0 2.0 35.0 94-95 23.24349813427302 30.5 2.0 34.0 2.0 35.0 96-97 22.250664053462422 30.0 2.0 34.0 2.0 35.0 98-99 21.082451265668844 29.0 2.0 34.0 2.0 35.0 100-101 17.98515019250506 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2572741306405142 1101 2 0.38619402947387726 1101 3 0.3630748454849666 1101 4 0.2951569983771307 1101 5 0.2643523022009475 1101 6 0.23954207095286506 1101 7 0.25081972676263575 1101 8 0.3143955018457021 1101 9 0.34177228588458775 1101 10-11 0.27104210370993087 1101 12-13 0.3544492456927486 1101 14-15 0.5026443796988858 1101 16-17 0.5249131807700351 1101 18-19 0.6178708301165798 1101 20-21 0.5577613869036 1101 22-23 0.4486866637612792 1101 24-25 0.41704136430188754 1101 26-27 0.45283819779578494 1101 28-29 0.42620527552524123 1101 30-31 0.5040602831327092 1101 32-33 0.6096411290274233 1101 34-35 0.3398113204392885 1101 36-37 0.5439432043379142 1101 38-39 0.5609349467886986 1101 40-41 0.8728650171706036 1101 42-43 0.6689340905409153 1101 44-45 0.7508718550227584 1101 46-47 0.7513268773761439 1101 48-49 0.7386385743891637 1101 50-51 0.8388451387180638 1101 52-53 0.8135810090147757 1101 54-55 1.1622589619749562 1101 56-57 1.2390777109745983 1101 58-59 1.4142181575826456 1101 60-61 1.2809708957040655 1101 62-63 1.308160419735941 1101 64-65 1.4922186322918911 1101 66-67 1.5035539105262323 1101 68-69 1.63967187184522 1101 70-71 1.7923811830093044 1101 72-73 1.7718166922452916 1101 74-75 1.987567844429158 1101 76-77 1.7759899670006831 1101 78-79 1.9313802548007786 1101 80-81 2.153441802735422 1101 82-83 2.368896762307987 1101 84-85 2.5193780515655853 1101 86-87 2.6270052254000156 1101 88-89 2.8339254290121687 1101 90-91 3.154486518910989 1101 92-93 3.463890474184975 1101 94-95 3.8307605887320086 1101 96-97 4.080925209765855 1101 98-99 3.9827436494961894 1101 100-101 4.218984827435861 1102 1 0.28150977167571156 1102 2 0.2827414823268981 1102 3 0.21227024545930817 1102 4 0.1381211166855394 1102 5 0.05997195134060718 1102 6 0.07559300567671556 1102 7 0.25255576436266836 1102 8 0.28456624275520426 1102 9 0.31651979574183287 1102 10-11 0.32476636886423904 1102 12-13 0.34409590168745297 1102 14-15 0.4603373898566403 1102 16-17 0.36387215226729097 1102 18-19 0.40193669716529 1102 20-21 0.3665649997830158 1102 22-23 0.25688737170059994 1102 24-25 0.3133840569865711 1102 26-27 0.2285667237311202 1102 28-29 0.25681047453758765 1102 30-31 0.21185709532023367 1102 32-33 0.2671058063184404 1102 34-35 0.3630358001493832 1102 36-37 0.46033122232899615 1102 38-39 0.6426854439956813 1102 40-41 0.6341320402295096 1102 42-43 0.6592326296023927 1102 44-45 0.5751256969618126 1102 46-47 0.45809578087911973 1102 48-49 0.4313166288389354 1102 50-51 0.5935724759068748 1102 52-53 0.6862173404203702 1102 54-55 0.5841476975746076 1102 56-57 0.7731592194250609 1102 58-59 0.7188829607632599 1102 60-61 0.8502765249023625 1102 62-63 0.7720635403693876 1102 64-65 0.8973309375564433 1102 66-67 1.1126616933717592 1102 68-69 1.1471721609741614 1102 70-71 1.2337034913136584 1102 72-73 1.3462887238390273 1102 74-75 1.5767867159857545 1102 76-77 1.6827915436539769 1102 78-79 1.9999508961108923 1102 80-81 2.2127129197337396 1102 82-83 2.2804628047299964 1102 84-85 2.382385283264984 1102 86-87 2.4220747395812374 1102 88-89 2.646666883277028 1102 90-91 2.7396992956927058 1102 92-93 2.9797341081976576 1102 94-95 3.279574338503707 1102 96-97 3.8812281157803064 1102 98-99 3.7372106126437856 1102 100-101 4.244350441251864 1103 1 0.187212811115689 1103 2 0.25016823576041247 1103 3 0.23136947400359276 1103 4 0.18541170882028268 1103 5 0.07903368654449139 1103 6 0.17278300076024777 1103 7 0.25466264820044415 1103 8 0.25572297882688133 1103 9 0.37014797186619575 1103 10-11 0.38946384900939535 1103 12-13 0.4006881682626826 1103 14-15 0.5502969151909483 1103 16-17 0.5748928358918306 1103 18-19 0.47015431818865494 1103 20-21 0.4506274878773624 1103 22-23 0.662697750945739 1103 24-25 0.5316802628419453 1103 26-27 0.4349817737998478 1103 28-29 0.3825610216682307 1103 30-31 0.6422029351015155 1103 32-33 0.7665654789048233 1103 34-35 0.7676436712109975 1103 36-37 0.8610966307312538 1103 38-39 0.7999349325441827 1103 40-41 0.6830832245050402 1103 42-43 0.6957599117524822 1103 44-45 1.0608900198713869 1103 46-47 1.086537079914514 1103 48-49 1.057643787260993 1103 50-51 1.0900384262088423 1103 52-53 1.1290929103040312 1103 54-55 0.9731033084832319 1103 56-57 1.1823059012342014 1103 58-59 1.4901212750868567 1103 60-61 1.4065972671253704 1103 62-63 1.5218305676373234 1103 64-65 1.497341701773518 1103 66-67 1.4162322688401012 1103 68-69 1.52352305509962 1103 70-71 1.5278952891011883 1103 72-73 1.6139763583539448 1103 74-75 1.6791571417226372 1103 76-77 1.7798152376309169 1103 78-79 2.002185075886622 1103 80-81 2.3966114047004226 1103 82-83 2.6515685619533613 1103 84-85 2.4926154016653257 1103 86-87 2.524231695170375 1103 88-89 3.0327633246147627 1103 90-91 3.418116193820275 1103 92-93 3.532262580458937 1103 94-95 3.5312746917272584 1103 96-97 3.9591903769190004 1103 98-99 4.1171917087434515 1103 100-101 4.255532527659501 1104 1 0.2403958039148968 1104 2 0.24863147527229756 1104 3 0.26158415880900066 1104 4 0.2293522097014815 1104 5 0.059378964252061905 1104 6 0.1285637708832681 1104 7 0.21089238552577427 1104 8 0.22605190212505022 1104 9 0.18436788269600157 1104 10-11 0.2656343088141355 1104 12-13 0.4073762214647161 1104 14-15 0.48233032112077723 1104 16-17 0.46157045551158404 1104 18-19 0.5393486471211943 1104 20-21 0.5437368223556192 1104 22-23 0.5870398524123317 1104 24-25 0.47123992873648035 1104 26-27 0.561389412936407 1104 28-29 0.5713026785534083 1104 30-31 0.6282933194705365 1104 32-33 0.617604162796134 1104 34-35 0.6088616303607566 1104 36-37 0.7161480311280499 1104 38-39 0.844948511205196 1104 40-41 0.8206400234727553 1104 42-43 0.7970738621675721 1104 44-45 0.9259352686095994 1104 46-47 0.9082041909317553 1104 48-49 1.0316521641349183 1104 50-51 1.0683148768980502 1104 52-53 1.2190860199847506 1104 54-55 1.2919655286433596 1104 56-57 1.4199523782266468 1104 58-59 1.513100836114667 1104 60-61 1.5539237193726834 1104 62-63 1.7564595002841656 1104 64-65 1.5520493364088601 1104 66-67 1.599777620875404 1104 68-69 1.7215773189607582 1104 70-71 1.6390478536964856 1104 72-73 1.9465309240272255 1104 74-75 1.99753490434248 1104 76-77 1.8109503629089225 1104 78-79 2.041685822049338 1104 80-81 2.348897674960213 1104 82-83 2.533390641675627 1104 84-85 2.7483472355174534 1104 86-87 2.7747248088906495 1104 88-89 2.8969666155789824 1104 90-91 3.1147015039365797 1104 92-93 3.3990572431737682 1104 94-95 3.4469132927500716 1104 96-97 3.886422199806635 1104 98-99 4.293268797219998 1104 100-101 4.4769665566034575 1105 1 0.3221368482863305 1105 2 0.3885561937641171 1105 3 0.3580146669591784 1105 4 0.28222470962172963 1105 5 0.15305021373369954 1105 6 0.2939597962748337 1105 7 0.25510432211067524 1105 8 0.2887971252581636 1105 9 0.3560161011399714 1105 10-11 0.3716026012636533 1105 12-13 0.44563103023054396 1105 14-15 0.47041606548264525 1105 16-17 0.4860130214626537 1105 18-19 0.5026568866184604 1105 20-21 0.29541949165926695 1105 22-23 0.38768740097847143 1105 24-25 0.3680149454834236 1105 26-27 0.47522239006485734 1105 28-29 0.53841486713997 1105 30-31 0.4714860847899942 1105 32-33 0.4191129828541946 1105 34-35 0.5010431991508213 1105 36-37 0.6692167095547958 1105 38-39 0.5607687925538798 1105 40-41 0.7377745413202135 1105 42-43 0.7797926091770435 1105 44-45 0.6353917106013753 1105 46-47 0.9540908220842681 1105 48-49 1.1000594498537595 1105 50-51 1.163916012500458 1105 52-53 0.9687271568631175 1105 54-55 1.1609804892481606 1105 56-57 1.1631738525958468 1105 58-59 1.5036188749675787 1105 60-61 1.536219805372241 1105 62-63 1.497958431669538 1105 64-65 1.6733131725758845 1105 66-67 1.6950996407165455 1105 68-69 1.6717272439982374 1105 70-71 1.7444818388315788 1105 72-73 1.8662492563504287 1105 74-75 2.1785454628929912 1105 76-77 2.0855524235807152 1105 78-79 2.2488136411185273 1105 80-81 2.521239695439334 1105 82-83 2.601981346323303 1105 84-85 2.4980521702841116 1105 86-87 2.6735892064790896 1105 88-89 3.0437416323259257 1105 90-91 3.025655601355954 1105 92-93 3.3638727077818906 1105 94-95 3.5500266296986176 1105 96-97 3.743905692103038 1105 98-99 3.9741198610497825 1105 100-101 4.476256805096231 1106 1 0.08092561436762225 1106 2 0.03988938813952814 1106 3 0.020099776659456836 1106 4 0.03190251706475067 1106 5 -0.04694635839181416 1106 6 0.012277528171921404 1106 7 0.0872838808105385 1106 8 -0.11538853063521515 1106 9 -0.03366853993669139 1106 10-11 0.1180615239932834 1106 12-13 0.0142808512899677 1106 14-15 -0.038349617734212416 1106 16-17 -0.004562811690533408 1106 18-19 0.13929085762859472 1106 20-21 0.3520338795542344 1106 22-23 0.2683932224492409 1106 24-25 0.09686343951312182 1106 26-27 -0.05830968627059008 1106 28-29 0.1644940256078442 1106 30-31 0.21217705582390778 1106 32-33 0.2469938763794346 1106 34-35 0.045825891259688945 1106 36-37 0.22591264768940533 1106 38-39 0.3097604722179099 1106 40-41 0.26733387699814415 1106 42-43 0.5687231731563926 1106 44-45 0.23170862533742564 1106 46-47 -0.017394238188273903 1106 48-49 0.5227995821365425 1106 50-51 0.3030125243064461 1106 52-53 0.561150669649372 1106 54-55 0.7611136787582069 1106 56-57 0.6609918999126805 1106 58-59 1.0989560810618926 1106 60-61 0.8073710349010526 1106 62-63 1.1072081086476544 1106 64-65 1.0016250383845495 1106 66-67 1.0205876567014975 1106 68-69 0.7820486846343684 1106 70-71 0.9695848271088003 1106 72-73 1.2231717106422089 1106 74-75 1.5514802376291144 1106 76-77 1.606211172594513 1106 78-79 1.8113449826724946 1106 80-81 1.490718343375061 1106 82-83 1.7379894537993295 1106 84-85 1.8054390739237967 1106 86-87 2.2417345218186604 1106 88-89 2.6432213141528322 1106 90-91 2.620524515565652 1106 92-93 2.0539229150853657 1106 94-95 2.6302720655113916 1106 96-97 3.2299829928840964 1106 98-99 3.2153902534474206 1106 100-101 3.7922426977340997 1107 1 0.2512122772849814 1107 2 0.34639284828558203 1107 3 0.14110000342368778 1107 4 0.14632976743423853 1107 5 0.12803543939715212 1107 6 0.08059304166014414 1107 7 0.08931997933986224 1107 8 0.14871107993571542 1107 9 0.2566942987691476 1107 10-11 0.26744365783941504 1107 12-13 0.21868119265776897 1107 14-15 0.3965650642143501 1107 16-17 0.42724063310777893 1107 18-19 0.301859305111428 1107 20-21 0.36062330511847307 1107 22-23 0.35803015007533645 1107 24-25 0.27369599457248484 1107 26-27 0.39033936713477857 1107 28-29 0.35731717658980955 1107 30-31 0.23524328000823402 1107 32-33 0.4448454047544317 1107 34-35 0.40853103455928874 1107 36-37 0.5278967550747495 1107 38-39 0.507189008292805 1107 40-41 0.532375329181427 1107 42-43 0.7989178133420936 1107 44-45 0.7528550539600545 1107 46-47 0.6331488010791162 1107 48-49 0.7597833416751101 1107 50-51 0.7619400516256931 1107 52-53 0.9410046589739309 1107 54-55 0.9057714692248595 1107 56-57 0.729420529985866 1107 58-59 0.6418089450623015 1107 60-61 0.945236519632175 1107 62-63 1.0623903086643338 1107 64-65 1.3826250633445447 1107 66-67 1.618174078037942 1107 68-69 1.5648404125725115 1107 70-71 1.692061251859986 1107 72-73 1.680679970011635 1107 74-75 1.9972063159939495 1107 76-77 1.844631725187618 1107 78-79 2.0192147637095843 1107 80-81 2.303326116130748 1107 82-83 2.468181438666136 1107 84-85 2.502935586162426 1107 86-87 2.7640673469239587 1107 88-89 3.084291821897601 1107 90-91 3.053259180700735 1107 92-93 3.38812666195275 1107 94-95 3.4215480683855084 1107 96-97 3.7902337718218924 1107 98-99 3.866037381215527 1107 100-101 4.2243669050550245 1108 1 0.07835262507787633 1108 2 0.15705412961676757 1108 3 0.10835373777218393 1108 4 0.12356981305332226 1108 5 -0.05943103373402181 1108 6 -0.0393959592729729 1108 7 0.11972368416216739 1108 8 0.10123477976937778 1108 9 0.09353696553198176 1108 10-11 0.15770692922035323 1108 12-13 0.2802609411517807 1108 14-15 0.3411775499583811 1108 16-17 0.3484417853051056 1108 18-19 0.38491653481913346 1108 20-21 0.27142042192044613 1108 22-23 0.2779848916198091 1108 24-25 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1.1014848364499343 2316 76-77 1.0009049695168528 2316 78-79 1.121736122835145 2316 80-81 1.3052904910348389 2316 82-83 1.4220588249210344 2316 84-85 1.8577514446472847 2316 86-87 2.1608488411879527 2316 88-89 2.1627940000836787 2316 90-91 2.5094379082725524 2316 92-93 2.315569167516731 2316 94-95 3.0149987021951397 2316 96-97 3.3202186510955514 2316 98-99 3.448979256830693 2316 100-101 3.7442921517809005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 15.0 5 49.0 6 123.0 7 315.0 8 668.0 9 1151.0 10 1915.0 11 2851.0 12 4116.0 13 6140.0 14 7554.0 15 9879.0 16 12689.0 17 15969.0 18 15491.0 19 13276.0 20 12765.0 21 14096.0 22 15483.0 23 17443.0 24 19889.0 25 22743.0 26 26912.0 27 31813.0 28 37850.0 29 44575.0 30 52240.0 31 60928.0 32 73012.0 33 88166.0 34 109536.0 35 138339.0 36 172009.0 37 196901.0 38 114517.0 39 7103.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.51840514454193 19.57367100322127 15.271449927921834 22.636473924314963 2 15.841177967415682 21.011032316504554 39.75773564133361 23.390054074746153 3 19.292120708769144 25.06890811139496 29.963627195438015 25.675343984397887 4 13.145588994287138 17.5218162511049 36.57314302324892 32.75945173135904 5 13.5097859023028 37.627287991911764 34.0831181865015 14.779807919283936 6 31.56682506065837 36.61014611400808 17.485035623817236 14.33799320151631 7 28.404432658824085 32.783922713223824 20.638158714339106 18.173485913612986 8 26.17683176422431 35.46356938190749 19.708780484911046 18.65081836895715 9 26.41019594582569 16.882456234019706 20.057009972295884 36.650337847858715 10-11 24.899261124656665 27.04058326956047 27.21907312819235 20.841082477590515 12-13 25.8316055337047 24.32797492984985 27.784612349985466 22.055807186459983 14-15 22.52969145799593 25.69086031073698 26.34764663308951 25.43180159817758 16-17 21.89763565822492 28.88766039616295 27.01359103501872 22.201112910593416 18-19 21.78165803508397 27.103392123013403 29.723010731637867 21.39193911026476 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26.896286551615717 29.224718651186798 21.943909695484773 46-47 22.18275967419483 27.140803063470308 28.57947433364775 22.09696292868711 48-49 22.046945724845312 27.099981016450432 28.61061921965747 22.242454039046788 50-51 22.25376259884794 27.235424193347452 28.439174037623022 22.07163917018159 52-53 22.472295882349453 27.20476131152598 28.239883844406084 22.083058961718486 54-55 22.119246353082154 27.294080395332422 28.360681509429487 22.225991742155937 56-57 22.04839173741005 27.448989125985513 28.2985771236363 22.204042012968138 58-59 22.159920091121037 27.043920221632938 28.4030237235046 22.393135963741422 60-61 21.994333113836042 27.29226361031519 28.509694958087884 22.20370831776089 62-63 21.94031156750728 27.374945867221932 28.506877087448906 22.177865477821875 64-65 21.74873344130227 27.196641394816307 28.40617528935082 22.648449874530602 66-67 21.737774696244443 27.380564835172983 28.250990890114085 22.63066957846849 68-69 21.96495078909852 27.150921464629143 28.39683137039708 22.487296375875257 70-71 21.87702070986611 27.196715549306806 28.188680168716296 22.737583572110793 72-73 21.924940769056793 27.252092085114587 28.054852117297663 22.76811502853096 74-75 21.80827949717323 27.509165495025716 27.9018505994649 22.78070440833615 76-77 21.848397076533367 27.132683146760634 28.271510734604043 22.747409042101953 78-79 21.86463690995272 27.26430736739694 28.228278666642936 22.642777056007404 80-81 21.811031300251273 27.338953998615533 28.117724746586205 22.73228995454699 82-83 21.962044371541584 27.334259285350925 28.04848567542836 22.655210667679132 84-85 21.889143249542634 27.251073246227786 28.039628351646556 22.82015515258302 86-87 21.864224281767093 27.3344886757166 27.928029140137546 22.87325790237876 88-89 21.765306969928872 27.364156388854283 27.81386629648745 23.056670344729397 90-91 21.689269156499996 27.228844277150426 28.058640409811026 23.02324615653856 92-93 21.705104732327648 27.04131081701375 28.061864262869356 23.19172018778925 94-95 21.524416107541807 26.96813433234263 28.0452653840906 23.462184176024962 96-97 21.65830204013834 26.837177502120817 27.94163448361779 23.56288597412305 98-99 21.46464758819935 26.873587356955987 27.802298195969556 23.859466858875106 100-101 21.461949080052815 26.613490185783984 28.128029922986425 23.796530811176776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1069.0 1 734.5 2 776.0 3 1035.0 4 707.0 5 569.5 6 939.5 7 1877.5 8 2758.5 9 2931.0 10 2385.5 11 1753.0 12 1637.0 13 1694.5 14 1761.0 15 1871.5 16 1936.0 17 1982.5 18 2113.0 19 2293.0 20 2508.5 21 2866.5 22 3442.5 23 4151.5 24 4896.5 25 6057.0 26 7579.5 27 9424.0 28 11231.5 29 13183.0 30 15526.5 31 18188.0 32 21699.0 33 24036.5 34 25729.5 35 28223.5 36 31083.5 37 33996.5 38 36180.0 39 37510.0 40 39062.5 41 42041.5 42 44647.5 43 46832.0 44 48756.0 45 50259.5 46 51059.0 47 51587.0 48 52296.0 49 51481.0 50 50019.5 51 48141.5 52 45457.5 53 44393.5 54 44387.5 55 41377.0 56 36804.0 57 33020.0 58 30201.5 59 26715.5 60 21852.5 61 17863.0 62 14706.0 63 11305.0 64 8351.0 65 6327.5 66 4663.5 67 3616.5 68 2799.5 69 2064.5 70 1611.0 71 1220.5 72 984.5 73 803.5 74 615.5 75 504.5 76 365.0 77 205.5 78 128.0 79 89.5 80 49.5 81 24.5 82 16.0 83 9.5 84 3.5 85 2.0 86 4.0 87 3.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04352868592310476 3 7.415449049932668E-5 4 0.0 5 0.10337135975606139 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011123173574899001 26-27 0.010307474179406407 28-29 0.0 30-31 0.0 32-33 3.707724524966334E-4 34-35 0.013607349006626445 36-37 0.002410020941228117 38-39 0.010604092141403714 40-41 0.005487432296950174 42-43 0.004300960448960947 44-45 0.002372943695978454 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.415449049932668E-5 68-69 2.595407167476434E-4 70-71 0.0 72-73 7.415449049932668E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.707724524966334E-5 82-83 0.0010381628669905735 84-85 0.004226805958461621 86-87 0.0013347808289878801 88-89 0.0 90-91 8.898538859919201E-4 92-93 2.2246347149798004E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 5.561586787449501E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1348536.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.29687087844172 #Duplication Level Percentage of deduplicated Percentage of total 1 82.53858025018403 51.41895276335614 2 10.541681229940492 13.13427508846591 3 2.886261061364877 5.394150979839656 4 1.2059500726648245 3.005076638505919 5 0.671469791280585 2.091523344309041 6 0.4019629899305415 1.5024621888969187 7 0.29826466314063704 1.3006668645091928 8 0.20566977909407405 1.0250066937456932 9 0.15949337563210944 0.8942344404948291 >10 0.989538241360158 11.425596494567852 >50 0.06688507096890137 2.8712380141102885 >100 0.031141314397258958 3.478763282386567 >500 0.0023862769550473057 1.0313863410258572 >1k 4.7725539100946113E-4 0.5717370809434198 >5k 2.3862769550473057E-4 0.8549297848428515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6137 0.45508610819436784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5365 0.39783884152888765 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3799 0.28171290940694205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1405 0.10418705915155398 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.415449049932668E-5 10-11 0.0 0.0 0.0 0.0 7.415449049932668E-5 12-13 0.0 0.0 0.0 0.0 7.415449049932668E-5 14-15 0.0 0.0 0.0 7.415449049932668E-5 7.415449049932668E-5 16-17 0.0 0.0 0.0 7.415449049932668E-5 7.415449049932668E-5 18-19 0.0 0.0 0.0 1.4830898099865337E-4 7.415449049932668E-5 20-21 0.0 0.0 0.0 1.853862262483167E-4 7.415449049932668E-5 22-23 0.0 0.0 0.0 4.078496977462968E-4 7.415449049932668E-5 24-25 0.0 0.0 0.0 7.415449049932668E-4 7.415449049932668E-5 26-27 0.0 0.0 0.0 0.0015572443004858603 7.415449049932668E-5 28-29 0.0 0.0 0.0 0.0035594155439676806 7.415449049932668E-5 30-31 0.0 0.0 0.0 0.010937787348650686 7.415449049932668E-5 32-33 0.0 0.0 0.0 0.027363006994251544 7.415449049932668E-5 34-35 0.0 0.0 0.0 0.04894196372955561 7.415449049932668E-5 36-37 0.0 0.0 0.0 0.08928200656118931 7.415449049932668E-5 38-39 0.0 0.0 0.0 0.1482719037534037 7.415449049932668E-5 40-41 0.0 0.0 0.0 0.21148860690407967 7.415449049932668E-5 42-43 0.0 0.0 0.0 0.27552100945024827 7.415449049932668E-5 44-45 0.0 0.0 0.0 0.35694264001850895 7.415449049932668E-5 46-47 0.0 0.0 0.0 0.4549007219681195 7.415449049932668E-5 48-49 0.0 0.0 0.0 0.5610528751179056 7.415449049932668E-5 50-51 0.0 0.0 0.0 0.6794405192000808 7.415449049932668E-5 52-53 0.0 0.0 0.0 0.7935642800785445 7.415449049932668E-5 54-55 0.0 0.0 0.0 0.9151776444974402 1.4830898099865337E-4 56-57 0.0 0.0 0.0 1.0610395273096156 1.4830898099865337E-4 58-59 0.0 0.0 0.0 1.21806166094194 1.4830898099865337E-4 60-61 0.0 0.0 0.0 1.3925471770868558 1.4830898099865337E-4 62-63 0.0 0.0 0.0 1.5666989980245245 1.4830898099865337E-4 64-65 0.0 0.0 0.0 1.7524559967253377 1.4830898099865337E-4 66-67 0.0 0.0 0.0 1.961682891669188 1.4830898099865337E-4 68-69 0.0 0.0 0.0 2.200089578624523 2.595407167476434E-4 70-71 0.0 0.0 0.0 2.448655430778266 2.9661796199730673E-4 72-73 0.0 0.0 0.0 2.7058973583204304 2.9661796199730673E-4 74-75 0.0 0.0 0.0 2.9751893905687354 2.9661796199730673E-4 76-77 0.0 0.0 0.0 3.2729567471687817 2.9661796199730673E-4 78-79 0.0 0.0 0.0 3.6011645221188013 2.9661796199730673E-4 80-81 0.0 0.0 0.0 3.951692798709119 2.9661796199730673E-4 82-83 0.0 0.0 0.0 4.320908006905266 2.9661796199730673E-4 84-85 0.0 0.0 0.0 4.687750271405435 2.9661796199730673E-4 86-87 0.0 0.0 0.0 5.075022098038168 2.9661796199730673E-4 88-89 0.0 0.0 0.0 5.489694008910403 2.9661796199730673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3260 0.0 48.29028 1 GTATCAA 5225 0.0 40.324295 1 TATCAAC 6840 0.0 30.525143 2 ATCAACG 6840 0.0 30.444305 3 TCAACGC 6980 0.0 29.833677 4 CAACGCA 7080 0.0 29.479448 5 AACGCAG 7250 0.0 28.759386 6 GTACATG 5935 0.0 25.723713 1 TACATGG 6005 0.0 24.790236 2 ACGCAGA 8515 0.0 24.375296 7 TGGTATC 1545 0.0 24.319122 2 CGCAGAG 8535 0.0 24.318178 8 ACATGGG 6040 0.0 23.378014 3 GTGGTAT 1725 0.0 23.160051 1 GCAGAGT 9390 0.0 21.800415 9 CATGGGG 2935 0.0 21.70625 4 CCATGCG 110 0.0028791802 21.588896 9 GAGTACT 5620 0.0 20.367315 12-13 AGAGTAC 8545 0.0 19.620756 10-11 CAGAGTA 9060 0.0 19.396645 10-11 >>END_MODULE