FastQCFastQC Report
Thu 26 May 2016
SRR938352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344860
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.4335767291762711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.35512990199723393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.257275850274378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.047.207681
GTATCAA49350.042.4142151
ATCAACG64950.032.120233
TATCAAC65150.032.021632
TCAACGC66250.031.5616784
CAACGCA67250.031.092365
AACGCAG70050.029.9796526
TATCGAC700.00837326627.155342
GTACATG56650.027.0171261
TGGTATC12850.026.6270242
TACATGG57100.026.216082
CGCAGAG80050.026.0545358
ACGCAGA80300.025.9734217
GTGGTAT14600.025.7993221
ACATGGG59350.024.5015743
GCAGAGT85100.023.948359
GTGTAAG5600.023.8398821
TACCTGG11350.022.6095122
GAGTACT51700.021.31873512-13
AGAGTAC78900.021.10410510-11
Produced by FastQC (version 0.11.5)