FastQCFastQC Report
Thu 26 May 2016
SRR938352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344860
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60620.450753238255283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.3845009889505227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.2880597236887111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14380.10692562794640335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.10417441220647503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30250.052.3149341
GTATCAA51150.040.3228231
ATCAACG66050.030.9945373
TCAACGC66400.030.9015464
TATCAAC67350.030.544142
CAACGCA67950.030.1966535
TGGTATC11850.029.6681822
AACGCAG70150.029.114236
GTGGTAT13250.027.6173291
ACCGTCC2101.2660166E-924.8792888
ACGCAGA82400.024.6130377
CGCAGAG82500.024.468068
TACATGG58000.023.8365062
GTACATG58850.023.8222261
GTAAGGT5950.023.1497124
TGGACCG1852.5439476E-723.1065625
ACATGGG61300.022.005873
GCAGAGT90400.021.9094859
TAAGGTG6800.021.6529645
GAGTACT53300.021.4760512-13
Produced by FastQC (version 0.11.5)