FastQCFastQC Report
Thu 26 May 2016
SRR938351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998570
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.27909955618267057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.21240186733514463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.14570417848761866No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24710.12363840145704179No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23390.11703367908054259No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23110.11563267736431548No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21750.10882781188549812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34000.046.5799751
GTATCAA57750.041.259091
ATCAACG75600.031.0164243
TATCAAC76100.030.937642
TCAACGC76950.030.4722774
CAACGCA77750.030.0363865
AACGCAG82650.028.5197856
ACGCAGA92500.025.3794257
CGCAGAG94450.024.9051368
TACATGG64900.023.378282
TGGTATC17300.023.3689632
GTACATG67250.023.3375661
GTGTAAG9400.023.2736191
TAAGGTG13250.022.6147215
GCAGAGT102700.022.117319
ACATGGG65900.021.9409163
TAAGGTA8150.021.5928864
GTGGTAT19750.021.4317111
AGAGTAC90300.021.1023810-11
GTAAGGT13600.020.983554
Produced by FastQC (version 0.11.5)