##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1998570 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.939608319948764 31.0 31.0 34.0 27.0 34.0 2 30.981331652131274 31.0 31.0 34.0 27.0 34.0 3 31.02032403168265 31.0 31.0 34.0 27.0 34.0 4 34.67170626998304 37.0 35.0 37.0 32.0 37.0 5 34.64144913613234 37.0 35.0 37.0 32.0 37.0 6 34.65577287760749 37.0 35.0 37.0 30.0 37.0 7 34.551074017922815 37.0 35.0 37.0 30.0 37.0 8 34.51324597086917 37.0 35.0 37.0 30.0 37.0 9 35.975318352622125 39.0 35.0 39.0 30.0 39.0 10-11 35.80640833195735 38.0 35.0 39.0 30.0 39.0 12-13 35.65557123343191 38.0 35.0 39.0 30.0 39.0 14-15 36.575802698929735 39.0 36.0 41.0 30.0 41.0 16-17 36.38045252355434 39.0 36.0 40.0 29.0 41.0 18-19 36.534230224610596 39.0 36.0 41.0 30.0 41.0 20-21 36.51317416953121 39.0 36.0 41.0 29.0 41.0 22-23 36.415354978809845 39.0 36.0 41.0 29.0 41.0 24-25 36.26017877782615 39.0 35.5 41.0 28.0 41.0 26-27 36.03591718078427 39.0 35.0 40.5 27.0 41.0 28-29 35.815827816889076 39.0 35.0 40.0 27.0 41.0 30-31 35.53526846695387 38.0 34.0 40.0 26.5 41.0 32-33 35.24821597442171 38.0 34.0 40.0 25.5 41.0 34-35 34.97640663074098 38.0 34.0 40.0 25.0 41.0 36-37 34.66964904907009 38.0 33.5 40.0 24.5 41.0 38-39 34.50199567690899 38.0 33.0 40.0 24.0 41.0 40-41 34.378657490105425 38.0 33.0 40.0 24.0 41.0 42-43 34.06264053798466 38.0 33.0 40.0 22.5 41.0 44-45 33.69285939446704 38.0 33.0 40.0 20.5 41.0 46-47 33.28743501603647 38.0 32.0 40.0 18.5 41.0 48-49 32.897080662673815 37.0 31.0 40.0 15.5 41.0 50-51 32.191866934858425 36.5 30.5 39.5 14.0 40.5 52-53 32.20250629199878 36.5 31.0 39.0 13.0 40.0 54-55 32.27575391404854 37.0 31.0 39.5 10.0 41.0 56-57 31.925355629274932 36.0 30.5 39.0 8.0 41.0 58-59 31.520107877132148 36.0 30.0 39.0 6.5 41.0 60-61 31.006992249458364 35.5 29.0 39.0 2.0 40.0 62-63 30.403799966976386 35.0 28.0 38.5 2.0 40.0 64-65 29.852266620633756 34.5 28.0 38.0 2.0 40.0 66-67 29.273329680721716 34.0 26.5 38.0 2.0 40.0 68-69 28.633189480478542 34.0 26.0 37.0 2.0 39.5 70-71 27.961415662198473 33.5 25.5 36.5 2.0 39.0 72-73 27.298132664855373 33.0 24.5 36.0 2.0 39.0 74-75 26.596251569872457 32.0 22.0 35.0 2.0 38.0 76-77 25.80705454399896 31.5 19.5 35.0 2.0 37.0 78-79 24.9938118254552 31.0 17.5 35.0 2.0 36.5 80-81 24.26775544514328 31.0 10.5 34.5 2.0 36.0 82-83 23.50073402482775 30.0 6.5 34.0 2.0 35.5 84-85 22.76112570487899 29.5 2.0 34.0 2.0 35.0 86-87 21.959574345657145 29.0 2.0 34.0 2.0 35.0 88-89 21.08699395067473 28.5 2.0 34.0 2.0 35.0 90-91 20.13970388828012 26.5 2.0 33.5 2.0 35.0 92-93 19.162659051221624 25.5 2.0 33.0 2.0 35.0 94-95 18.133655313549188 24.5 2.0 32.5 2.0 34.0 96-97 16.86935083584763 18.5 2.0 32.0 2.0 34.0 98-99 15.349863402332668 2.0 2.0 31.5 2.0 34.0 100-101 12.017985609710944 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.310932280729606 1101 2 0.2555046770560061 1101 3 0.2628366794646446 1101 4 0.1807665578009292 1101 5 0.06538345019441039 1101 6 0.4024714242483327 1101 7 0.27874992595457115 1101 8 0.4508257475702777 1101 9 0.5621610597164377 1101 10-11 0.5879152517265709 1101 12-13 0.6189050881913758 1101 14-15 0.8293009010266488 1101 16-17 0.7004031561997408 1101 18-19 0.6904508870443422 1101 20-21 0.767519653403312 1101 22-23 0.743028118648354 1101 24-25 0.8000662756816084 1101 26-27 0.791253929159403 1101 28-29 0.8223220672086313 1101 30-31 0.8723976594332754 1101 32-33 0.8085146208022138 1101 34-35 0.8502839138330032 1101 36-37 0.6868414279158586 1101 38-39 0.7769216616809373 1101 40-41 0.785929096281258 1101 42-43 0.9976655335033726 1101 44-45 0.8575500176541979 1101 46-47 0.877577562579674 1101 48-49 0.6909802376471035 1101 50-51 0.8814789247620638 1101 52-53 0.9780399188919873 1101 54-55 0.9645708122563548 1101 56-57 1.0606756828093573 1101 58-59 1.1384144602263966 1101 60-61 1.0426727767262065 1101 62-63 1.0056007035443066 1101 64-65 1.0642692988459643 1101 66-67 1.1064466311468948 1101 68-69 1.0307602811135546 1101 70-71 0.9893049428912803 1101 72-73 0.8879324411228389 1101 74-75 0.7112610893216349 1101 76-77 0.9196386644635943 1101 78-79 1.3045664956726988 1101 80-81 1.1502820946680181 1101 82-83 1.160473415158176 1101 84-85 1.1352279121823905 1101 86-87 1.4073709749822712 1101 88-89 1.3855164092902754 1101 90-91 1.1735695942216253 1101 92-93 1.3307622197373092 1101 94-95 1.2659771721679007 1101 96-97 1.1311712098662419 1101 98-99 1.1620062273322382 1101 100-101 0.8715680402054211 1102 1 0.4703380367650922 1102 2 0.377769143945315 1102 3 0.28598255686069507 1102 4 0.3278019506398877 1102 5 0.34725413569309893 1102 6 0.3008759504329106 1102 7 0.37843814156691025 1102 8 0.6221962555163998 1102 9 0.7142231166811328 1102 10-11 0.6517136374040646 1102 12-13 0.7164189357294362 1102 14-15 0.9245414509085066 1102 16-17 0.8822453603924814 1102 18-19 0.7828781920254997 1102 20-21 0.8390210087385839 1102 22-23 0.8454527785649049 1102 24-25 0.7703046126649227 1102 26-27 0.7014810610658557 1102 28-29 0.6691733468888046 1102 30-31 0.6977374825471827 1102 32-33 0.7291189451704412 1102 34-35 0.7380019624417926 1102 36-37 0.8171756849390803 1102 38-39 0.7002486322801147 1102 40-41 0.8663718634353899 1102 42-43 1.0787135934942782 1102 44-45 1.1157262872591716 1102 46-47 1.1058567918453406 1102 48-49 1.1341287913486795 1102 50-51 1.3415118943469437 1102 52-53 1.2688249407450058 1102 54-55 1.1816085169234327 1102 56-57 1.394302160114389 1102 58-59 1.276649806412241 1102 60-61 1.4643486646712773 1102 62-63 1.4953115063422437 1102 64-65 1.5345440835699904 1102 66-67 1.4003718862029757 1102 68-69 1.4147986699567987 1102 70-71 1.588014797006597 1102 72-73 1.7001200970020172 1102 74-75 1.7205664578655693 1102 76-77 1.77362953586659 1102 78-79 1.7628668958688891 1102 80-81 1.933434959616644 1102 82-83 2.104408979718059 1102 84-85 1.9865567968086282 1102 86-87 2.0103212633880396 1102 88-89 1.9427341553828192 1102 90-91 2.26049407137878 1102 92-93 2.370986206724421 1102 94-95 2.135698628119762 1102 96-97 2.3083100934615324 1102 98-99 2.353049663666887 1102 100-101 1.5443935940288416 1103 1 0.4462099732828406 1103 2 0.2584131582926581 1103 3 0.2917804839502445 1103 4 0.27191963037815015 1103 5 0.35849544764077024 1103 6 0.46310326351794373 1103 7 0.1734960652827553 1103 8 0.4366226900235617 1103 9 0.5121931351355116 1103 10-11 0.5744575215505776 1103 12-13 0.6546265681853001 1103 14-15 0.7436471358063201 1103 16-17 0.8816602647014307 1103 18-19 0.9309351793862177 1103 20-21 0.7348840766500828 1103 22-23 0.8956846451438878 1103 24-25 0.8807235583512849 1103 26-27 0.8382351208774352 1103 28-29 0.7263801500592848 1103 30-31 0.924920770779913 1103 32-33 0.9890163299416912 1103 34-35 0.7612110948840254 1103 36-37 0.7430493386129413 1103 38-39 0.8142321920255213 1103 40-41 0.8764770746001318 1103 42-43 0.895224857533286 1103 44-45 0.6923430149858874 1103 46-47 0.3814560540742704 1103 48-49 0.6757289182162154 1103 50-51 0.7417610334961822 1103 52-53 0.7786377303257055 1103 54-55 0.7224664788207491 1103 56-57 0.8482457606271119 1103 58-59 0.7796346277623449 1103 60-61 1.0179855508005886 1103 62-63 0.7458455923960088 1103 64-65 0.9295331695277937 1103 66-67 0.9300155561090087 1103 68-69 1.0885208280381917 1103 70-71 1.2359672398631751 1103 72-73 1.1027308308438286 1103 74-75 1.1263875160579708 1103 76-77 1.0354657919006272 1103 78-79 0.9738162407738358 1103 80-81 1.198154270249173 1103 82-83 1.0050773069756893 1103 84-85 1.0252774043990662 1103 86-87 1.1097420767341752 1103 88-89 1.116319193498633 1103 90-91 1.1756746404443135 1103 92-93 0.9778574573898204 1103 94-95 1.1570873225659781 1103 96-97 1.1675411711588026 1103 98-99 1.1732775605849959 1103 100-101 1.124757762435392 1104 1 0.4895576478805168 1104 2 0.34722889710839766 1104 3 0.40519100986077206 1104 4 0.3043049002634177 1104 5 0.43755142669674996 1104 6 0.49170397961865575 1104 7 0.5675463655830555 1104 8 0.6251395722904434 1104 9 0.6482291711715504 1104 10-11 0.7283946317376788 1104 12-13 0.7880778078865447 1104 14-15 0.9399311602153446 1104 16-17 1.0837007667419343 1104 18-19 0.9844752579262988 1104 20-21 0.857277383418392 1104 22-23 1.0256090406932827 1104 24-25 0.8992550706327904 1104 26-27 1.0365769723442853 1104 28-29 0.9395555851097228 1104 30-31 0.8754236775328224 1104 32-33 1.068179868480236 1104 34-35 0.8838385300114666 1104 36-37 1.2233885720771696 1104 38-39 1.3796187324120623 1104 40-41 1.1417216460947088 1104 42-43 1.077096151904584 1104 44-45 1.0673747775176565 1104 46-47 0.9388627396059519 1104 48-49 0.8176465570088567 1104 50-51 0.8255077609929131 1104 52-53 0.8905912704042507 1104 54-55 1.1936022780815563 1104 56-57 1.1047881202944758 1104 58-59 1.2200524494301739 1104 60-61 1.3370144354544777 1104 62-63 1.4050343484597647 1104 64-65 1.477075423320045 1104 66-67 1.338182858026311 1104 68-69 1.4307089296012911 1104 70-71 1.6460280217989585 1104 72-73 1.5814239470369458 1104 74-75 1.4010768688723232 1104 76-77 1.5507204696553067 1104 78-79 1.9895413408739344 1104 80-81 2.0279992594691585 1104 82-83 1.8873855767839594 1104 84-85 1.9720330542797164 1104 86-87 2.225254945372047 1104 88-89 2.151858097787539 1104 90-91 2.2280426334373082 1104 92-93 1.8802355566429192 1104 94-95 1.6878618470905025 1104 96-97 2.0324351516777845 1104 98-99 2.0311717528541884 1104 100-101 1.8188114314890615 1105 1 0.5524561046365761 1105 2 0.413500096019483 1105 3 0.4351541301933324 1105 4 0.3915823346893461 1105 5 0.31623295559348463 1105 6 0.4002060765174562 1105 7 0.6038776663960661 1105 8 0.48393758023659217 1105 9 0.6838102328645164 1105 10-11 0.7058895274445618 1105 12-13 0.6266513189044574 1105 14-15 0.635399720528774 1105 16-17 0.7229937071826527 1105 18-19 0.7477767699006961 1105 20-21 0.7581939719111972 1105 22-23 0.6669376054212393 1105 24-25 0.773316294559514 1105 26-27 0.8740083402948429 1105 28-29 0.6928196885935094 1105 30-31 0.7713694432687603 1105 32-33 0.8550297608762136 1105 34-35 0.7287668859692289 1105 36-37 1.0353462984134794 1105 38-39 0.8978534747142461 1105 40-41 1.2161815567005974 1105 42-43 1.2143082881662153 1105 44-45 1.1706714151220368 1105 46-47 1.0688547152892838 1105 48-49 1.2344623288147645 1105 50-51 1.446412600414611 1105 52-53 1.2485161453260716 1105 54-55 1.242214347205632 1105 56-57 1.3231413879617726 1105 58-59 1.3161058191833312 1105 60-61 1.2201816493381585 1105 62-63 1.3018578456822958 1105 64-65 1.2757269242581337 1105 66-67 1.3063635683967618 1105 68-69 1.4045929977688978 1105 70-71 1.6591493890137592 1105 72-73 1.4835930416916945 1105 74-75 1.5632709561695286 1105 76-77 1.7339871252856653 1105 78-79 1.6977805206922056 1105 80-81 1.8422136811665446 1105 82-83 1.827712711390145 1105 84-85 1.8766258834376437 1105 86-87 1.9157212984235876 1105 88-89 1.9067225811043187 1105 90-91 1.653005270931331 1105 92-93 1.5728523805769967 1105 94-95 1.6154951565118303 1105 96-97 2.0183191370730675 1105 98-99 2.3629992047542743 1105 100-101 1.8687111850114828 1106 1 0.30271829355218216 1106 2 0.045484754864745014 1106 3 0.03154620461704383 1106 4 -0.007995446304796872 1106 5 0.11352520805741051 1106 6 -0.09714650633026167 1106 7 0.24999616969667215 1106 8 0.10718159368726532 1106 9 0.013459337674980532 1106 10-11 0.002489128083645653 1106 12-13 0.26246236676546886 1106 14-15 0.39547648719492656 1106 16-17 0.1808453459629149 1106 18-19 0.024279748244993016 1106 20-21 0.1759475691337542 1106 22-23 0.24376926257228604 1106 24-25 0.04427079804935374 1106 26-27 0.1283933006046638 1106 28-29 0.12191427380104614 1106 30-31 -0.11718867461062388 1106 32-33 0.05595839443517292 1106 34-35 -0.3419346561559493 1106 36-37 -0.160795250344286 1106 38-39 0.013004176546779433 1106 40-41 0.18987249744706958 1106 42-43 -0.15294133020194778 1106 44-45 -0.04763030799428947 1106 46-47 -0.05156119432006534 1106 48-49 0.20461799338345088 1106 50-51 -0.08074977309885156 1106 52-53 -0.04109311707409091 1106 54-55 0.14328984871366401 1106 56-57 -0.7782994917751402 1106 58-59 -0.43130133268389415 1106 60-61 0.3673233803049243 1106 62-63 0.3736791066154197 1106 64-65 0.40774718177706504 1106 66-67 -0.06297032889450804 1106 68-69 0.040588713749563965 1106 70-71 -0.1914128826687609 1106 72-73 0.24318372252101383 1106 74-75 0.7009113542938543 1106 76-77 0.5413490012608193 1106 78-79 0.6987939118827526 1106 80-81 0.692327924804804 1106 82-83 0.49294102769934867 1106 84-85 0.41909311040869923 1106 86-87 0.35099728885129267 1106 88-89 0.348811970310706 1106 90-91 0.2685696902443624 1106 92-93 0.4072167873368109 1106 94-95 0.43259127901998085 1106 96-97 0.4452047308987588 1106 98-99 0.3423040797584296 1106 100-101 -0.04669184584085784 1107 1 0.18173948319406108 1107 2 0.0249578177444576 1107 3 0.05246253791274569 1107 4 0.11197970912231625 1107 5 0.1856256576454527 1107 6 0.28005110263097066 1107 7 0.32260644491110213 1107 8 0.21628359217629622 1107 9 0.45233556407868747 1107 10-11 0.30546734612082815 1107 12-13 0.28760287160024234 1107 14-15 0.4867287615291005 1107 16-17 0.5122409153474123 1107 18-19 0.38315162821956505 1107 20-21 0.4657253939356423 1107 22-23 0.5287964083303152 1107 24-25 0.46124154717538346 1107 26-27 0.40640034629884525 1107 28-29 0.54926754982489 1107 30-31 0.5028840583792302 1107 32-33 0.6037331232632468 1107 34-35 0.615054205376687 1107 36-37 0.6088137264258577 1107 38-39 0.6844852028524997 1107 40-41 0.7430378724488449 1107 42-43 0.8058691696008324 1107 44-45 0.7687948751521745 1107 46-47 0.8459909342647691 1107 48-49 0.6867082830219928 1107 50-51 0.727648227269519 1107 52-53 0.6958565123368388 1107 54-55 0.9365842363356194 1107 56-57 0.8460103240706367 1107 58-59 0.6286724196372866 1107 60-61 0.6044503403685084 1107 62-63 0.7045189180782003 1107 64-65 0.7370872238154007 1107 66-67 0.8841188661385928 1107 68-69 0.8905218491246067 1107 70-71 1.0189978206072432 1107 72-73 1.108062107057048 1107 74-75 1.0565646600708583 1107 76-77 1.102125311896593 1107 78-79 1.0764828364408991 1107 80-81 1.1872665743404767 1107 82-83 1.2338037001911175 1107 84-85 1.4398660481057668 1107 86-87 1.4844372338605076 1107 88-89 1.4181187765627996 1107 90-91 1.115821156535766 1107 92-93 1.330538533239352 1107 94-95 1.3102866021068351 1107 96-97 1.6308933474134513 1107 98-99 1.5408491685905759 1107 100-101 1.3510281298630158 1108 1 0.3968268505208812 1108 2 0.20243836341001398 1108 3 0.1548061131874796 1108 4 0.21492870478654424 1108 5 0.08298517383698112 1108 6 0.2554707162949512 1108 7 0.3406905635352757 1108 8 0.465365962794138 1108 9 0.37343638716374983 1108 10-11 0.3174503925297927 1108 12-13 0.3580321922393921 1108 14-15 0.5359367437955882 1108 16-17 0.5442040859483299 1108 18-19 0.6601616022986505 1108 20-21 0.6404128379808895 1108 22-23 0.6498489752467833 1108 24-25 0.5906384952286672 1108 26-27 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-3.9561341371951215 2316 90-91 -3.7664995987488297 2316 92-93 -3.694124388731627 2316 94-95 -3.9956785067631095 2316 96-97 -3.981279122717236 2316 98-99 -3.7076010155189802 2316 100-101 -3.068897841787347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8722.0 3 3191.0 4 3344.0 5 3878.0 6 4665.0 7 5637.0 8 7188.0 9 9014.0 10 11064.0 11 13654.0 12 15803.0 13 18600.0 14 20796.0 15 23451.0 16 26534.0 17 29578.0 18 29050.0 19 28633.0 20 30230.0 21 32605.0 22 35700.0 23 39304.0 24 43632.0 25 47408.0 26 53311.0 27 59658.0 28 66732.0 29 75389.0 30 85276.0 31 97782.0 32 114453.0 33 132886.0 34 159389.0 35 187165.0 36 208684.0 37 187060.0 38 75987.0 39 3117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.761139928003914 18.26542876901106 14.624142206779082 23.349289096205943 2 15.46223917568119 19.42679386770124 40.27936472951413 24.83160222710344 3 19.517008067065404 23.473993069627845 29.184511514968133 27.824487348338618 4 13.080363673527462 16.146880178911026 36.396109166184054 34.37664698137746 5 14.152203472569925 36.95320405545807 33.982629632469724 14.911962839502275 6 33.10389558353617 35.448708317055825 17.040498442367603 14.4068976570404 7 29.3937381351941 32.03253603172323 20.096785285113196 18.476940547969477 8 26.69932188782346 34.796278566019716 19.31881482267434 19.185584723482478 9 26.295978587701143 16.957325544616296 19.760833269939905 36.98586259774266 10-11 25.282403959875598 26.2467413002259 27.058315551022194 21.412539188876302 12-13 26.354427331626027 23.41059091247014 27.261813606088225 22.973168149815606 14-15 22.88642983286132 25.004460685036094 25.501504871218213 26.607604610884373 16-17 22.61517216387827 28.06313259476596 26.42077618645773 22.90091905489804 18-19 22.681019738142876 26.605447873593395 28.574056452900603 22.139475935363127 20-21 23.039708494629902 25.86476822359159 28.87720065927156 22.218322622506953 22-23 22.707355168153274 25.793404006067387 28.819314533375014 22.67992629240433 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26.72379757134858 27.441724425575952 23.00463422359866 50-51 22.97948529207557 26.869906382395275 27.47103864074954 22.679569684779615 52-53 23.247639261727755 26.548932291516802 27.5210002559588 22.682428190796642 54-55 22.714635003147603 26.796685859205567 27.707113783098624 22.78156535454821 56-57 22.755737888681157 26.916657992609704 27.480563440050926 22.847040678658217 58-59 22.76894797462802 26.475905551453227 27.48459417117054 23.270552302748214 60-61 22.394545770757716 26.750321871093913 27.779833915766744 23.075298442381634 62-63 22.598628473977136 26.739428070445836 27.8384553718603 22.823488083716732 64-65 22.3442679964071 26.79876500273971 27.692336561773633 23.164630439079556 66-67 22.260347662649735 26.817802873736348 27.648278050054692 23.273571413559225 68-69 22.842121144716966 26.391782602157637 27.77807941619705 22.98801683692835 70-71 22.64930838568437 26.481221819841682 27.419398834967783 23.45007095950616 72-73 22.622324379040723 26.701818177268215 27.42405909858147 23.251798345109588 74-75 22.612935798987227 27.01610249339021 27.306920856413907 23.064040851208656 76-77 22.82573390567391 26.70349645239929 27.322881461540426 23.147888180386374 78-79 22.834243784836218 26.932251299626365 27.26360660889215 22.96989830664527 80-81 22.970345284487916 26.898629350082164 27.009639383767965 23.121385981661955 82-83 22.94767955481799 26.9833549624499 27.10767547168112 22.961290011050988 84-85 22.849191796446842 27.070009114447487 27.075163043029548 23.005636046076123 86-87 23.090213139890068 27.031789830617043 26.791436972805048 23.086560056687844 88-89 23.01491482427517 27.079313308416058 26.575236596454822 23.33053527085395 90-91 22.90008534152879 27.071334005721138 26.68759744826326 23.34098320448681 92-93 23.033486713899574 26.73016106586105 26.92067929897899 23.31567292126039 94-95 22.83331535370763 26.574420757782402 27.042149665741654 23.550114222768315 96-97 23.182849814488147 26.24557244373074 26.98048671582948 23.591091025951634 98-99 22.94984167086659 26.479352649804582 26.57852419330218 23.99228148602665 100-101 23.56157846494427 25.814836597036166 27.016439067490584 23.60714587052898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 238.0 1 182.0 2 188.5 3 245.5 4 223.5 5 230.5 6 385.5 7 812.0 8 1328.0 9 1558.5 10 1401.5 11 1200.0 12 1209.5 13 1264.0 14 1367.0 15 1600.0 16 1788.5 17 1916.0 18 2130.5 19 2434.0 20 2883.5 21 3407.5 22 4084.0 23 5114.5 24 6339.0 25 7884.0 26 9942.0 27 12511.5 28 15149.0 29 17960.5 30 21053.0 31 25337.5 32 31758.0 33 34846.5 34 35256.5 35 38139.5 36 42249.5 37 46340.0 38 49985.5 39 52873.0 40 55954.5 41 60048.0 42 62887.5 43 64726.5 44 67043.5 45 70423.0 46 72941.0 47 74780.5 48 75767.0 49 74537.5 50 72852.5 51 71194.5 52 69838.5 53 69496.0 54 70084.5 55 67218.0 56 62254.5 57 57774.5 58 54028.0 59 48528.5 60 39976.0 61 33373.0 62 28297.0 63 21918.5 64 15925.0 65 12239.5 66 9387.5 67 7080.5 68 5500.0 69 4338.0 70 3384.5 71 2486.0 72 1933.0 73 1543.5 74 1165.5 75 894.0 76 685.5 77 475.5 78 308.5 79 209.5 80 131.0 81 78.0 82 52.5 83 30.5 84 22.0 85 16.0 86 11.5 87 8.5 88 5.0 89 5.0 90 5.0 91 3.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006404579274181039 2 0.11283067393186128 3 0.12153689888270112 4 0.12478922429537119 5 0.16676924000660473 6 0.09756976238010177 7 0.02696928303737172 8 0.02616871062809909 9 0.023316671420065348 10-11 0.0516619382858744 12-13 0.053763440860215055 14-15 0.027944980661172736 16-17 0.03242318257554151 18-19 0.03817729676718854 20-21 0.060743431553560795 22-23 0.03379916640397884 24-25 0.05221233181724933 26-27 0.058366732213532675 28-29 0.05536458567876032 30-31 0.06952471016776995 32-33 0.05173699194924371 34-35 0.06392070330286155 36-37 0.053963583962533215 38-39 0.03199787848311543 40-41 0.03737672435791591 42-43 0.03269837934122898 44-45 0.007480348449141135 46-47 0.013059337426259775 48-49 0.008856332277578469 50-51 0.008731242838629619 52-53 0.010082208779277183 54-55 0.01120801372981682 56-57 0.013709802508793788 58-59 0.01908864838359427 60-61 0.024392440595025443 62-63 0.010607584422862347 64-65 0.009256618482214785 66-67 0.009807012013589717 68-69 0.011233031617606588 70-71 0.02999644745993385 72-73 0.027644766007695505 74-75 0.029396018152979382 76-77 0.02296642099100857 78-79 0.00555397108932887 80-81 0.005103649109113016 82-83 0.005328810099220943 84-85 0.00472838079226647 86-87 0.01290923009952116 88-89 0.03797715366487038 90-91 0.03577557953937065 92-93 0.029946411684354312 94-95 0.0469585753813977 96-97 0.04273055234492663 98-99 0.025868495974621854 100-101 0.07647968297332593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1998570.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.61278712207192 #Duplication Level Percentage of deduplicated Percentage of total 1 80.46841259924042 48.77414762928811 2 11.517352050993889 13.96197616153702 3 3.5161753661331794 6.39375566853911 4 1.5123982494499724 3.6668269255082167 5 0.7880977803362749 2.388440149545002 6 0.47539380920962976 1.7288966254064502 7 0.31829739278582153 1.350502447730626 8 0.2185962093138626 1.0599780400666423 9 0.16249867910494353 0.8864548059785238 >10 0.9251364228589902 10.196349834665167 >50 0.062212219284725555 2.56942734915526 >100 0.030298919373818952 3.3379947874362834 >500 0.002730644567406799 1.1250802083593652 >1k 0.0023169105420421266 2.280403969038657 >5k 8.27468050729331E-5 0.2797653977455656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5578 0.27909955618267057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4245 0.21240186733514463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.14570417848761866 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2471 0.12363840145704179 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2339 0.11703367908054259 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2311 0.11563267736431548 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2175 0.10882781188549812 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.003577557953937E-5 0.0 0.0 0.0 0.0 2 5.003577557953937E-5 0.0 0.0 0.0 0.0 3 5.003577557953937E-5 0.0 0.0 0.0 0.0 4 5.003577557953937E-5 0.0 0.0 0.0 0.0 5 5.003577557953937E-5 0.0 0.0 0.0 0.0 6 5.003577557953937E-5 0.0 0.0 0.0 0.0 7 5.003577557953937E-5 0.0 0.0 0.0 0.0 8 5.003577557953937E-5 0.0 0.0 1.0007155115907874E-4 0.0 9 5.003577557953937E-5 0.0 0.0 2.0014310231815748E-4 0.0 10-11 5.003577557953937E-5 0.0 0.0 2.0014310231815748E-4 0.0 12-13 7.505366336930906E-5 0.0 0.0 2.0014310231815748E-4 7.505366336930906E-5 14-15 1.0007155115907874E-4 1.2508943894884843E-4 0.0 2.0014310231815748E-4 1.501073267386181E-4 16-17 1.0007155115907874E-4 1.751252145283878E-4 0.0 2.0014310231815748E-4 2.0014310231815748E-4 18-19 1.2508943894884843E-4 2.0014310231815748E-4 0.0 2.0014310231815748E-4 2.0014310231815748E-4 20-21 1.501073267386181E-4 2.0014310231815748E-4 0.0 2.0014310231815748E-4 2.0014310231815748E-4 22-23 2.0014310231815748E-4 2.0014310231815748E-4 0.0 2.5017887789769686E-4 2.0014310231815748E-4 24-25 2.5017887789769686E-4 2.0014310231815748E-4 0.0 3.502504290567756E-4 2.0014310231815748E-4 26-27 2.5017887789769686E-4 2.0014310231815748E-4 0.0 7.755545214828603E-4 2.2516099010792717E-4 28-29 2.5017887789769686E-4 2.0014310231815748E-4 0.0 0.0023516814522383505 2.5017887789769686E-4 30-31 2.5017887789769686E-4 2.0014310231815748E-4 0.0 0.008030741980516069 2.5017887789769686E-4 32-33 2.5017887789769686E-4 2.0014310231815748E-4 0.0 0.020364560660872524 2.5017887789769686E-4 34-35 3.502504290567756E-4 2.5017887789769686E-4 0.0 0.03975342369794403 2.5017887789769686E-4 36-37 5.003577557953937E-4 3.002146534772362E-4 0.0 0.0737777510920308 2.5017887789769686E-4 38-39 6.754829703237814E-4 3.002146534772362E-4 0.0 0.12363840145704179 2.5017887789769686E-4 40-41 7.005008581135512E-4 3.002146534772362E-4 0.0 0.18185502634383585 2.5017887789769686E-4 42-43 7.505366336930905E-4 4.0028620463631497E-4 0.0 0.24029681222073782 3.002146534772362E-4 44-45 7.505366336930905E-4 4.5032198021585434E-4 0.0 0.31412459908834817 3.002146534772362E-4 46-47 7.505366336930905E-4 4.7533986800562405E-4 0.0 0.4046142992239451 3.002146534772362E-4 48-49 7.505366336930905E-4 5.003577557953937E-4 0.0 0.5011333103168766 3.002146534772362E-4 50-51 7.505366336930905E-4 5.003577557953937E-4 0.0 0.6123878573179824 3.502504290567756E-4 52-53 7.505366336930905E-4 5.003577557953937E-4 0.0 0.7186388267611342 3.502504290567756E-4 54-55 8.005724092726299E-4 5.003577557953937E-4 0.0 0.8360227562707336 3.502504290567756E-4 56-57 8.005724092726299E-4 5.003577557953937E-4 0.0 0.9740214253191032 3.502504290567756E-4 58-59 8.005724092726299E-4 5.003577557953937E-4 0.0 1.128181649879664 3.502504290567756E-4 60-61 8.005724092726299E-4 5.253756435851634E-4 0.0 1.2912732603811725 3.502504290567756E-4 62-63 9.006439604317087E-4 5.50393531374933E-4 0.0 1.4560660872523856 4.0028620463631497E-4 64-65 9.006439604317087E-4 5.50393531374933E-4 0.0 1.6301905862691823 4.0028620463631497E-4 66-67 9.006439604317087E-4 5.50393531374933E-4 0.0 1.8338361928779077 4.0028620463631497E-4 68-69 9.006439604317087E-4 5.50393531374933E-4 0.0 2.0591723082003632 4.0028620463631497E-4 70-71 9.006439604317087E-4 5.754114191647028E-4 0.0 2.2996192277478396 4.0028620463631497E-4 72-73 9.256618482214783E-4 6.254471947442421E-4 0.0 2.546320619242759 4.2530409242608463E-4 74-75 9.50679736011248E-4 6.504650825340118E-4 0.0 2.7922214383284047 4.5032198021585434E-4 76-77 9.50679736011248E-4 6.504650825340118E-4 0.0 3.0609635889661106 4.5032198021585434E-4 78-79 9.50679736011248E-4 6.504650825340118E-4 0.0 3.3630796019153695 4.7533986800562405E-4 80-81 9.50679736011248E-4 6.504650825340118E-4 0.0 3.6819325817959845 5.003577557953937E-4 82-83 9.756976238010178E-4 6.754829703237814E-4 0.0 4.022926392370545 5.50393531374933E-4 84-85 0.0010007155115907874 7.005008581135512E-4 0.0 4.361968807697504 5.50393531374933E-4 86-87 0.0010007155115907874 7.005008581135512E-4 0.0 4.7185237444773005 5.754114191647028E-4 88-89 0.0010007155115907874 7.005008581135512E-4 0.0 5.104024377429862 6.004293069544725E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3400 0.0 46.579975 1 GTATCAA 5775 0.0 41.25909 1 ATCAACG 7560 0.0 31.016424 3 TATCAAC 7610 0.0 30.93764 2 TCAACGC 7695 0.0 30.472277 4 CAACGCA 7775 0.0 30.036386 5 AACGCAG 8265 0.0 28.519785 6 ACGCAGA 9250 0.0 25.379425 7 CGCAGAG 9445 0.0 24.905136 8 TACATGG 6490 0.0 23.37828 2 TGGTATC 1730 0.0 23.368963 2 GTACATG 6725 0.0 23.337566 1 GTGTAAG 940 0.0 23.273619 1 TAAGGTG 1325 0.0 22.614721 5 GCAGAGT 10270 0.0 22.11731 9 ACATGGG 6590 0.0 21.940916 3 TAAGGTA 815 0.0 21.592886 4 GTGGTAT 1975 0.0 21.431711 1 AGAGTAC 9030 0.0 21.10238 10-11 GTAAGGT 1360 0.0 20.98355 4 >>END_MODULE