FastQCFastQC Report
Thu 26 May 2016
SRR938351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998570
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.2777485902420231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.23411739393666472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.1666191326798661No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23770.1189350385525651No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23740.11878493122582648No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23610.11813446614329244No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23250.11633317822242904No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21730.10872774033433906No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20350.10182280330436261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.047.264231
GTATCAA59700.041.1672551
ATCAACG77350.031.7576563
TATCAAC78500.031.4897612
TCAACGC78300.031.433034
CAACGCA79600.030.9196765
AACGCAG83150.029.6381846
ACGCAGA94850.025.7319247
CGCAGAG95400.025.5338028
TAAGGTG13250.025.1015425
TGGTATC17050.023.6990132
GCAGAGT107000.022.3662559
GTAAGGT13600.022.009994
GTACATG67400.021.7233371
TACATGG66750.021.5075722
GTGGTAT20650.021.409111
ACATGGG67350.021.0231063
AGAGTAC92450.021.00694510-11
GAGTACT62400.020.54592312-13
AGGTAAG13250.020.0912912
Produced by FastQC (version 0.11.5)