##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1998570 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.823897586774542 31.0 31.0 34.0 30.0 34.0 2 32.05537058997183 33.0 31.0 34.0 30.0 34.0 3 32.11789529513602 33.0 31.0 34.0 30.0 34.0 4 35.77720770350801 37.0 35.0 37.0 35.0 37.0 5 35.67434415607159 37.0 35.0 37.0 33.0 37.0 6 35.67491656534422 37.0 35.0 37.0 33.0 37.0 7 35.61496870262238 37.0 35.0 37.0 33.0 37.0 8 35.59255167444723 37.0 35.0 37.0 33.0 37.0 9 37.18939441700816 39.0 37.0 39.0 33.0 39.0 10-11 37.08817429462066 39.0 37.0 39.0 33.0 39.0 12-13 36.978005774128505 39.0 37.0 39.0 33.0 39.0 14-15 38.03612683068394 40.0 37.0 41.0 33.0 41.0 16-17 37.94560961087177 40.0 37.0 41.0 33.0 41.0 18-19 38.18528698019084 40.0 37.5 41.0 33.0 41.0 20-21 38.15481469250514 40.0 37.0 41.0 33.0 41.0 22-23 38.05121536898883 40.0 37.0 41.0 33.0 41.0 24-25 37.92167875030647 40.0 37.0 41.0 32.5 41.0 26-27 37.72990538234838 40.0 37.0 41.0 32.0 41.0 28-29 37.61553710903296 40.0 37.0 41.0 32.0 41.0 30-31 37.3676000840601 39.0 36.0 41.0 31.5 41.0 32-33 37.171813596721655 39.0 36.0 41.0 31.0 41.0 34-35 37.06148546210541 39.0 36.0 41.0 31.0 41.0 36-37 37.00967091470401 39.0 36.0 41.0 31.0 41.0 38-39 36.84456936709748 39.0 35.5 41.0 30.0 41.0 40-41 36.66780523073998 39.0 35.0 41.0 30.0 41.0 42-43 36.46052327414101 39.0 35.0 40.5 30.0 41.0 44-45 36.170855911977064 39.0 35.0 40.0 30.0 41.0 46-47 35.80125739904031 38.0 35.0 40.0 28.5 41.0 48-49 35.44362794397995 38.0 34.5 40.0 27.5 41.0 50-51 35.05842427335545 38.0 34.0 40.0 26.5 41.0 52-53 34.56621759558084 38.0 33.0 40.0 24.5 41.0 54-55 34.22658675953306 37.5 33.0 40.0 24.0 41.0 56-57 33.87945681162031 37.0 33.0 40.0 23.0 41.0 58-59 33.38990303066693 36.5 32.0 39.5 22.5 41.0 60-61 32.98193633447915 36.0 31.5 39.0 22.0 40.0 62-63 32.49518680856812 35.5 31.0 39.0 20.0 40.0 64-65 31.956471126855703 35.0 31.0 38.5 18.0 40.0 66-67 31.448273515563628 35.0 30.0 38.0 17.0 40.0 68-69 30.936278439083946 34.0 29.0 37.5 13.0 39.5 70-71 30.349535167644866 34.0 29.0 37.0 8.0 39.0 72-73 29.838111499722302 34.0 28.5 36.0 7.5 39.0 74-75 29.239536768789684 34.0 27.5 36.0 6.5 38.5 76-77 28.034566465022493 31.5 26.0 34.5 2.0 36.5 78-79 28.71006669768885 33.0 28.0 35.0 2.0 37.0 80-81 28.313969238005175 33.0 26.5 35.0 2.0 37.0 82-83 27.859693180624145 33.0 26.0 35.0 2.0 36.0 84-85 27.291211216019455 32.5 26.0 35.0 2.0 36.0 86-87 26.67171902910581 32.0 25.0 34.5 2.0 35.0 88-89 25.897685845379446 31.5 23.5 34.0 2.0 35.0 90-91 25.271919922744765 31.0 20.5 34.0 2.0 35.0 92-93 24.434089874260096 31.0 18.0 34.0 2.0 35.0 94-95 23.604605292784342 30.5 6.0 34.0 2.0 35.0 96-97 22.516036215894363 30.0 2.0 34.0 2.0 35.0 98-99 21.13687311427671 29.0 2.0 34.0 2.0 35.0 100-101 17.193642454354865 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18956844118345728 1101 2 0.1901550010085984 1101 3 0.22022532844148657 1101 4 0.17854220734876236 1101 5 0.058493299306206836 1101 6 0.10046290452946494 1101 7 0.17449478018048836 1101 8 0.1800860065009715 1101 9 0.24716644738891347 1101 10-11 0.24348064307567086 1101 12-13 0.26001077570595044 1101 14-15 0.4125010382818388 1101 16-17 0.3949365312196207 1101 18-19 0.4113778204259475 1101 20-21 0.35845948992149346 1101 22-23 0.3684253326356739 1101 24-25 0.2596557816410936 1101 26-27 0.39041460133763195 1101 28-29 0.12070127098701278 1101 30-31 0.2181005381914929 1101 32-33 0.2427556662221022 1101 34-35 0.1873388269589853 1101 36-37 0.3092750122520087 1101 38-39 0.35525774072382177 1101 40-41 0.22186783224358209 1101 42-43 0.38453756349092316 1101 44-45 0.42431443816893477 1101 46-47 0.2683654858776521 1101 48-49 0.19975073840726765 1101 50-51 0.34938240746856053 1101 52-53 0.449142428827912 1101 54-55 0.507153152021786 1101 56-57 0.4289155897354888 1101 58-59 0.6277389888913163 1101 60-61 0.6064318679185945 1101 62-63 0.6678295479020875 1101 64-65 0.6556800339122226 1101 66-67 0.18095260471965702 1101 68-69 0.41905270313365506 1101 70-71 0.516341073838749 1101 72-73 0.39986733170194455 1101 74-75 0.41523491776599997 1101 76-77 0.31319322337818534 1101 78-79 0.48511312714931165 1101 80-81 0.5048557555174078 1101 82-83 0.41061668580445243 1101 84-85 0.5345233595351182 1101 86-87 0.43113250823952143 1101 88-89 0.46950046792255407 1101 90-91 0.352986594743804 1101 92-93 0.7986078548781173 1101 94-95 0.912147065273512 1101 96-97 0.6418753676121796 1101 98-99 0.7244432082538417 1101 100-101 0.8210534695144354 1102 1 0.32729664309144013 1102 2 0.2888096628308432 1102 3 0.21803117445082165 1102 4 0.2765710093948357 1102 5 0.1103974854854286 1102 6 0.06570165285156548 1102 7 0.20354675361737407 1102 8 0.23693302700230134 1102 9 0.31876386855505956 1102 10-11 0.39102604394354046 1102 12-13 0.3471466497592175 1102 14-15 0.43210757971407787 1102 16-17 0.3829725603319929 1102 18-19 0.40539987026770774 1102 20-21 0.5180204091959908 1102 22-23 0.30489119307831203 1102 24-25 0.40699108731787703 1102 26-27 0.4071873309297587 1102 28-29 0.3641700961930212 1102 30-31 0.46301659904463577 1102 32-33 0.49470822376007106 1102 34-35 0.4523081744090476 1102 36-37 0.566798346616288 1102 38-39 0.5490857844486143 1102 40-41 0.5194638954710342 1102 42-43 0.4423090549012372 1102 44-45 0.5782696949224473 1102 46-47 0.7263690973280674 1102 48-49 0.5284846272041719 1102 50-51 0.7377666687093054 1102 52-53 0.7069276662432884 1102 54-55 0.6407919174222556 1102 56-57 0.8216147427941252 1102 58-59 0.7079544783480287 1102 60-61 0.6978927870889535 1102 62-63 0.6196086662624865 1102 64-65 0.6914579172148869 1102 66-67 0.7928825750720279 1102 68-69 0.8885178273319347 1102 70-71 0.8709257830742914 1102 72-73 0.7704226598930255 1102 74-75 0.940281284499001 1102 76-77 0.8965872161656989 1102 78-79 1.0989245819452655 1102 80-81 1.0597478754117304 1102 82-83 1.0495312616725094 1102 84-85 1.0579970062565387 1102 86-87 1.033119795267563 1102 88-89 1.0419268148488108 1102 90-91 0.9796052649427764 1102 92-93 1.4413460590875182 1102 94-95 1.6349593937738724 1102 96-97 1.2964223794701937 1102 98-99 1.2438867762169004 1102 100-101 1.5330231158359737 1103 1 0.2365999928589666 1103 2 0.20353215525263124 1103 3 0.1683902115908893 1103 4 0.16909622886253572 1103 5 0.05908987111020991 1103 6 0.019770757273846584 1103 7 0.12948227541566126 1103 8 0.11379525754768594 1103 9 0.23063133642656197 1103 10-11 0.24814552467553597 1103 12-13 0.2728440424091829 1103 14-15 0.33746702594132927 1103 16-17 0.2963975898014226 1103 18-19 0.3211637673635934 1103 20-21 0.36663794701635766 1103 22-23 0.2483338789528844 1103 24-25 0.24162769163157805 1103 26-27 0.38841957225281476 1103 28-29 0.3697607019349931 1103 30-31 0.21625116559908975 1103 32-33 0.2188021461385503 1103 34-35 0.20790660836268415 1103 36-37 0.39368527390684704 1103 38-39 0.36467660230986354 1103 40-41 0.299082818127296 1103 42-43 0.3687284106848665 1103 44-45 0.28397363296745226 1103 46-47 0.20286577564109365 1103 48-49 0.4488752499156803 1103 50-51 0.3576708817013099 1103 52-53 0.17108960820543473 1103 54-55 0.21306727047460328 1103 56-57 0.4530736487232687 1103 58-59 0.3890141959869595 1103 60-61 0.34180966057192563 1103 62-63 0.3664778866433096 1103 64-65 0.467477125121075 1103 66-67 0.5422310658433176 1103 68-69 0.5021675517283768 1103 70-71 0.3685934810447158 1103 72-73 0.28641796902657646 1103 74-75 0.35902545753177506 1103 76-77 0.38291309805162044 1103 78-79 0.6409787450892352 1103 80-81 0.5394104340349344 1103 82-83 0.8198409255240477 1103 84-85 0.9010431300796569 1103 86-87 0.8119033719825239 1103 88-89 0.7479259522303927 1103 90-91 0.8503190665344782 1103 92-93 1.1715310877084697 1103 94-95 1.0580297901036069 1103 96-97 1.1355819224642438 1103 98-99 1.1723710122187931 1103 100-101 1.2832819518667584 1104 1 0.2174178299268057 1104 2 0.2397809040013783 1104 3 0.17825055270122903 1104 4 0.2325808673471741 1104 5 0.09951298653331975 1104 6 0.013734913738005616 1104 7 0.16195657663995888 1104 8 0.2336617586641836 1104 9 0.19350238054760638 1104 10-11 0.2904077388127462 1104 12-13 0.2657239415390862 1104 14-15 0.3018491965734995 1104 16-17 0.4002884999423273 1104 18-19 0.3691088278086525 1104 20-21 0.3828854445138532 1104 22-23 0.3454215916656054 1104 24-25 0.3192562817601683 1104 26-27 0.3109192353774759 1104 28-29 0.27479698822127574 1104 30-31 0.20323117445408911 1104 32-33 0.3156963961578043 1104 34-35 0.47400876821484417 1104 36-37 0.48103416125573517 1104 38-39 0.36065262641088935 1104 40-41 0.5847134006328787 1104 42-43 0.691426108382565 1104 44-45 0.5782893429081568 1104 46-47 0.5406706478209671 1104 48-49 0.48798479215022184 1104 50-51 0.6891706507010795 1104 52-53 0.6592943995352272 1104 54-55 0.6743497067570345 1104 56-57 0.5524494668490831 1104 58-59 0.6048059492787132 1104 60-61 0.7163873632746274 1104 62-63 0.719998715164138 1104 64-65 0.5975776583466121 1104 66-67 0.5233042062914635 1104 68-69 0.7805271613379894 1104 70-71 0.7453045671307983 1104 72-73 0.6906734264070344 1104 74-75 0.8607675842739013 1104 76-77 0.9974451782087002 1104 78-79 1.1165439737794642 1104 80-81 1.0121771911766935 1104 82-83 1.4020466889798122 1104 84-85 1.4324201802067087 1104 86-87 1.4628054760096276 1104 88-89 1.373040249219688 1104 90-91 1.5510970079374218 1104 92-93 1.7832976619750447 1104 94-95 1.4250097508335635 1104 96-97 1.4465664646987868 1104 98-99 1.5578269007996859 1104 100-101 1.4458741113339144 1105 1 0.24883245487622574 1105 2 0.2320513487646494 1105 3 0.19397246529365475 1105 4 0.22013650874069413 1105 5 0.1528450322972219 1105 6 0.21625992937277516 1105 7 0.23681701330993832 1105 8 0.2432640376620867 1105 9 0.3541034455514662 1105 10-11 0.31765190336412275 1105 12-13 0.3704527702187761 1105 14-15 0.4479789008004005 1105 16-17 0.37632292403091583 1105 18-19 0.3679146071560453 1105 20-21 0.3820694473832944 1105 22-23 0.29055400438678447 1105 24-25 0.30615204381733463 1105 26-27 0.16155784682580787 1105 28-29 0.28242192844126635 1105 30-31 0.4011720980092548 1105 32-33 0.49069168273397423 1105 34-35 0.5504740682185272 1105 36-37 0.6096034876729775 1105 38-39 0.4809216134616179 1105 40-41 0.4854032849355647 1105 42-43 0.6195107568761102 1105 44-45 0.7947365479627919 1105 46-47 0.7391739633122754 1105 48-49 0.817775355185951 1105 50-51 0.8457138026710638 1105 52-53 0.7951365389238134 1105 54-55 0.7729287819143096 1105 56-57 0.9368820625890706 1105 58-59 0.9924788514602199 1105 60-61 0.9165831428059548 1105 62-63 1.0600430443404605 1105 64-65 1.1401372314253742 1105 66-67 0.9566831793628694 1105 68-69 0.8767221637291875 1105 70-71 0.9748852103637056 1105 72-73 1.0336487004753856 1105 74-75 1.0942729000962892 1105 76-77 0.8183657673636873 1105 78-79 1.245464507752903 1105 80-81 1.1666456319301055 1105 82-83 1.2959019937658915 1105 84-85 1.3321711734745598 1105 86-87 1.3793818978541594 1105 88-89 1.4222742603663612 1105 90-91 1.5623047250768067 1105 92-93 1.7055335263020126 1105 94-95 1.651820855200267 1105 96-97 1.8967584504987371 1105 98-99 1.8399130162026935 1105 100-101 1.6926019605411398 1106 1 -0.0989005419559632 1106 2 0.15502331340392317 1106 3 0.03711299902082743 1106 4 0.02203878944466453 1106 5 -0.03745868528164209 1106 6 -0.12290619980075945 1106 7 -0.027931259568880762 1106 8 0.08665891454454311 1106 9 -0.19462153642670188 1106 10-11 0.04429132767736377 1106 12-13 0.087799301291966 1106 14-15 0.09709565555036903 1106 16-17 0.05625220445525514 1106 18-19 0.0538381655619915 1106 20-21 0.10115797144823802 1106 22-23 0.08200643495649018 1106 24-25 0.14775492562365145 1106 26-27 0.05606469043662088 1106 28-29 0.2116297502193163 1106 30-31 0.15112139331945684 1106 32-33 0.002800150019020009 1106 34-35 0.12551888273304002 1106 36-37 0.21650466528647172 1106 38-39 0.1385516036579304 1106 40-41 0.047338419468104576 1106 42-43 0.17293933071361067 1106 44-45 0.018701504815787473 1106 46-47 -0.5544088839352241 1106 48-49 -0.23325546061855107 1106 50-51 0.13333832191331396 1106 52-53 0.09869228433896637 1106 54-55 0.2151788335558038 1106 56-57 0.13415433763940854 1106 58-59 0.11060625817647463 1106 60-61 0.012435967584131902 1106 62-63 -0.34229503907378955 1106 64-65 -0.23321205573460801 1106 66-67 -0.27684388569979745 1106 68-69 -0.5015920844994284 1106 70-71 -0.2796495219975057 1106 72-73 0.11400486052140124 1106 74-75 -0.0035528406488758435 1106 76-77 0.046585585426360154 1106 78-79 0.15895926233518054 1106 80-81 -0.23934775608466552 1106 82-83 0.2649059709808661 1106 84-85 0.3108853915379832 1106 86-87 0.6862348533169396 1106 88-89 0.7730475478710837 1106 90-91 0.8290534326341685 1106 92-93 0.8818633592552381 1106 94-95 1.0593496702187828 1106 96-97 0.9081524851915148 1106 98-99 1.1353133865225793 1106 100-101 1.4418450203220878 1107 1 0.20118496980979117 1107 2 0.18139896279745926 1107 3 0.16056484072305466 1107 4 0.0384039406807446 1107 5 0.1347053839632366 1107 6 0.1760913335664256 1107 7 0.04460154774444902 1107 8 0.08802637153614512 1107 9 0.2322526497604258 1107 10-11 0.23576707062652957 1107 12-13 0.3080071803488025 1107 14-15 0.43567197377296907 1107 16-17 0.3754695455649326 1107 18-19 0.3579809158232976 1107 20-21 0.3361373110560848 1107 22-23 0.39945069805850864 1107 24-25 0.2857747318122179 1107 26-27 0.24544925709398058 1107 28-29 0.32741889676070457 1107 30-31 0.3612022295888835 1107 32-33 0.27457537153380684 1107 34-35 0.27632512020162636 1107 36-37 0.317449682272283 1107 38-39 0.2787323986568353 1107 40-41 0.28363082857357824 1107 42-43 0.34337431183566736 1107 44-45 0.20110950425637242 1107 46-47 0.19646063041187745 1107 48-49 0.3114820237450431 1107 50-51 0.3501980565663274 1107 52-53 0.4293869863114921 1107 54-55 0.1801027847569685 1107 56-57 0.25320707659818 1107 58-59 0.34049145486039123 1107 60-61 0.3331264556684701 1107 62-63 0.5311198968289546 1107 64-65 0.2408135740291506 1107 66-67 0.37140636807041716 1107 68-69 0.6388420236804961 1107 70-71 0.7765414430057405 1107 72-73 0.6556402010579525 1107 74-75 0.7064323604683054 1107 76-77 0.6802531352423351 1107 78-79 0.9136673226074912 1107 80-81 0.8160880682720659 1107 82-83 0.9759974320072011 1107 84-85 1.0079471643052038 1107 86-87 1.2367783169227948 1107 88-89 1.2808264448835693 1107 90-91 1.2979228765974575 1107 92-93 1.6085170704247354 1107 94-95 1.6133611363822205 1107 96-97 1.7050249072128736 1107 98-99 1.7514827884840933 1107 100-101 1.7313362824455893 1108 1 0.11494225516027612 1108 2 0.15120825651234782 1108 3 0.21054694658038642 1108 4 0.08682551348601919 1108 5 0.020216770803656914 1108 6 0.12042724018289164 1108 7 0.06557512106481767 1108 8 0.1573675963418637 1108 9 0.04870879955522156 1108 10-11 0.1454204202439513 1108 12-13 0.17558381289639868 1108 14-15 0.3233394171038242 1108 16-17 0.2915720670578068 1108 18-19 0.2924684460061613 1108 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66-67 -1.0053311594939913 2316 68-69 -1.0712087814637563 2316 70-71 -0.881360180809093 2316 72-73 -1.0901066408959963 2316 74-75 -1.1246205733755268 2316 76-77 -1.1228017782400848 2316 78-79 -1.3255191391255678 2316 80-81 -1.324155143175819 2316 82-83 -1.3384845109157872 2316 84-85 -1.4634705595673339 2316 86-87 -1.6312562199894813 2316 88-89 -1.718843653051092 2316 90-91 -1.4870821980359565 2316 92-93 -1.7357266305041676 2316 94-95 -1.574084874245667 2316 96-97 -2.0555255689340868 2316 98-99 -1.5878045778162253 2316 100-101 -1.8531138962353424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 22.0 5 50.0 6 146.0 7 360.0 8 845.0 9 1605.0 10 2677.0 11 4102.0 12 5695.0 13 7793.0 14 10529.0 15 13655.0 16 16965.0 17 20918.0 18 20877.0 19 18639.0 20 18746.0 21 20041.0 22 21593.0 23 24166.0 24 27849.0 25 32247.0 26 38294.0 27 45956.0 28 54541.0 29 64355.0 30 76001.0 31 90069.0 32 107657.0 33 131108.0 34 166069.0 35 214627.0 36 279738.0 37 307048.0 38 147191.0 39 6381.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.21866300003402 19.22585416328924 15.336211399785046 23.219271436891688 2 16.025431471445913 20.229333800578214 39.2350033166043 24.51023141137157 3 19.560335639982586 23.680781758957657 29.70368813701797 27.055194464041787 4 13.584362819415881 16.782049165153083 36.166108767768954 33.46747924766208 5 14.093049589437046 37.105549946110955 34.107436688504286 14.693963775947713 6 33.16891577477897 35.89686625937546 16.675973320924463 14.25824464492112 7 29.310857262943003 32.21103088708426 20.002852039208033 18.475259810764697 8 26.83778901914869 35.10169771386541 19.114416808017733 18.94609645896816 9 26.155651290672832 16.857853365156085 19.66110769199978 37.3253876521713 10-11 25.307494858824057 26.43152353933062 27.00713510159764 21.25384650024768 12-13 26.24443977443872 23.471832360137498 27.351181094482556 22.932546770941222 14-15 22.70045582591553 25.125364635714536 25.519846690383623 26.65433284798631 16-17 22.52015190861466 28.182775684614498 26.478056810619595 22.81901559615125 18-19 22.561656584457886 26.672595906072843 28.67775459453509 22.08799291493418 20-21 22.924265850082808 25.926762635284227 28.966110769199975 22.182860745432983 22-23 22.713840395883057 25.807402292639235 28.92017792721796 22.558579384259748 24-25 22.68532290852228 26.04454730258014 28.86865363565285 22.401476153244722 26-27 22.210196704774717 26.406860449290676 28.69141688282576 22.691525963108848 28-29 22.37110389594144 26.7658277155582 28.53304392597296 22.3300244625274 30-31 22.47286446430068 26.30089828411597 28.44219446018516 22.78404279139819 32-33 22.143876983520396 26.32643528858131 28.422642985967656 23.107044741930633 34-35 22.94875811053852 26.38275736791695 28.2662588586647 22.40222566287983 36-37 22.972764328449603 25.9232974983941 28.376907721924344 22.72703045123196 38-39 22.443110314725292 26.33490670126642 28.285320842463218 22.93666214154507 40-41 23.017481726697035 26.06960367147283 28.375182132592524 22.537732469237607 42-43 22.65751733013367 26.188116136663623 28.169735737352532 22.984630795850176 44-45 22.64011303067886 26.426350539720488 28.32755077761134 22.605985651989307 46-47 23.05015586144093 26.66421491366327 27.621124103734168 22.66450512116163 48-49 22.79042015040754 26.72603411439179 27.51129557633708 22.972250158863588 50-51 22.95539100885165 26.885881771050023 27.571927610680618 22.586799609417707 52-53 23.299909435246203 26.52814262197471 27.5466958875596 22.625252055219484 54-55 22.781354428134428 26.78851768058929 27.67166524854349 22.758462642732784 56-57 22.819415882355884 26.93175620568707 27.420255482670107 22.82857242928694 58-59 22.756453967971954 26.56418559200838 27.49895212558978 23.180408314429886 60-61 22.395512791646027 26.818475209774988 27.74954092175906 23.036471076819925 62-63 22.580690359541514 26.842350331983976 27.784745976021892 22.79221333245261 64-65 22.32711366133427 26.900100162316498 27.59863410928477 23.174152067064462 66-67 22.274388120286304 26.84322008454628 27.603691293974475 23.278700501192937 68-69 22.81626083962559 26.50163345050405 27.69992253120771 22.982183178662652 70-71 22.61142847707535 26.591701362324745 27.379640132514815 23.417230028085097 72-73 22.58145703250676 26.7301754405277 27.36570516204344 23.3226623649221 74-75 22.518133449576926 27.078133609728827 27.19541985573316 23.208313084961084 76-77 22.579939461097133 26.762795682829815 27.443827051873576 23.213437804199476 78-79 22.64521936593556 26.97467881477603 27.351139404535097 23.028962414753313 80-81 22.76561375850053 26.944664451993518 27.08262482561013 23.20709696389582 82-83 22.717931969111678 27.01151367037191 27.18742843842795 23.083125922088453 84-85 22.56490082249036 27.19267582980297 27.16257464930123 23.07984869840544 86-87 22.773329823787964 27.15574950353027 26.90034000388819 23.170580668793576 88-89 22.61083333103809 27.254118730511824 26.63733262356066 23.497715314889426 90-91 22.452442643142525 27.267836167164067 26.849705789763174 23.43001539993023 92-93 22.571057714757167 26.910731395920024 27.019786279454266 23.49842460986854 94-95 22.293554219505634 26.7456034434219 27.18726734692601 23.77357499014646 96-97 22.551781512276943 26.427529909614172 27.200617288585416 23.820071289523465 98-99 22.22839949636164 26.764674224579654 26.80237200651826 24.204554272540445 100-101 22.299964469265916 26.461489188147745 27.28995581177719 23.94859053080915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 264.0 1 198.5 2 170.5 3 231.0 4 212.5 5 198.0 6 384.0 7 887.5 8 1422.5 9 1639.5 10 1404.5 11 1129.0 12 1133.0 13 1261.5 14 1427.0 15 1523.0 16 1668.0 17 1886.0 18 2145.5 19 2444.0 20 2835.5 21 3320.5 22 4025.5 23 5022.0 24 6196.0 25 7648.5 26 9706.5 27 12468.5 28 15032.5 29 17838.5 30 21151.5 31 25566.0 32 32398.5 33 35572.5 34 35605.5 35 38094.0 36 42071.0 37 46411.0 38 50335.0 39 53508.5 40 56618.0 41 60930.0 42 63746.5 43 65329.5 44 67886.0 45 71594.0 46 73845.0 47 75808.5 48 76759.5 49 75487.5 50 74106.5 51 71952.5 52 70391.0 53 70352.0 54 71266.0 55 67707.0 56 62161.5 57 57266.5 58 53307.5 59 47808.5 60 38633.5 61 32233.5 62 27427.0 63 20381.0 64 14145.0 65 10692.0 66 8070.5 67 6201.5 68 4862.0 69 3859.0 70 3038.0 71 2127.0 72 1569.0 73 1323.0 74 1065.5 75 775.0 76 554.5 77 375.0 78 237.0 79 153.5 80 90.5 81 55.0 82 34.0 83 17.0 84 8.0 85 5.0 86 3.5 87 1.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.002146534772362E-4 2 0.05228738548061864 3 0.0 4 0.0 5 0.04738387947382379 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006629740264288967 26-27 0.0021015025743406535 28-29 1.501073267386181E-4 30-31 0.0018763415842327263 32-33 4.253040924260847E-4 34-35 0.012208729241407605 36-37 0.023691939736911895 38-39 0.004853470231215319 40-41 0.0013009301650680235 42-43 0.0014010017162271024 44-45 0.008731242838629619 46-47 0.0 48-49 0.0 50-51 7.755545214828603E-4 52-53 0.0 54-55 0.001826305808653187 56-57 0.0 58-59 0.02389208283923005 60-61 0.0 62-63 0.01028235188159534 64-65 0.015686215644185594 66-67 0.006904937029976433 68-69 0.017312378350520623 70-71 5.0035775579539364E-5 72-73 5.0035775579539364E-5 74-75 0.023366707195644886 76-77 0.050611186998704066 78-79 0.030947127195945102 80-81 0.026644050496104715 82-83 0.03640102673411489 84-85 0.015486072541867435 86-87 0.010532530759493038 88-89 0.08438533551489315 90-91 0.025468209769985543 92-93 0.02469265524850268 94-95 0.027894944885593197 96-97 0.04007865623921104 98-99 0.05528953201539101 100-101 0.01501073267386181 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1998570.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.656123383415384 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39669520524771 45.20049531605771 2 12.6859461454631 14.628449523963665 3 3.852922558435048 6.664337352476267 4 1.682315462382456 3.8798315147580156 5 0.8801291079877134 2.5372416221737457 6 0.5268722366128404 1.822644640886757 7 0.36252136344134245 1.4631103521788607 8 0.2725487709494633 1.2571284452688376 9 0.18776607884775545 0.9743277788339659 >10 1.0392696806437263 11.022167494798904 >50 0.07166323331923617 2.820694152705641 >100 0.03579217661714416 3.801836801346086 >500 0.0029526769028624168 1.1933817257978012 >1k 0.002518459711264997 2.4564116004831016 >5k 8.684343831948265E-5 0.2779416782706883 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.2777485902420231 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4679 0.23411739393666472 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.1666191326798661 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2377 0.1189350385525651 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2374 0.11878493122582648 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2361 0.11813446614329244 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2325 0.11633317822242904 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2173 0.10872774033433906 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2035 0.10182280330436261 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.003577557953937E-5 0.0 5.003577557953937E-5 0.0 6 0.0 5.003577557953937E-5 0.0 5.003577557953937E-5 0.0 7 1.0007155115907874E-4 5.003577557953937E-5 0.0 5.003577557953937E-5 0.0 8 1.0007155115907874E-4 5.003577557953937E-5 0.0 1.0007155115907874E-4 0.0 9 1.0007155115907874E-4 5.003577557953937E-5 0.0 1.0007155115907874E-4 0.0 10-11 1.0007155115907874E-4 5.003577557953937E-5 0.0 1.2508943894884843E-4 0.0 12-13 1.0007155115907874E-4 5.003577557953937E-5 0.0 1.501073267386181E-4 1.0007155115907874E-4 14-15 1.0007155115907874E-4 1.0007155115907874E-4 0.0 1.751252145283878E-4 1.501073267386181E-4 16-17 1.0007155115907874E-4 1.501073267386181E-4 0.0 2.0014310231815748E-4 1.501073267386181E-4 18-19 1.751252145283878E-4 1.501073267386181E-4 0.0 2.0014310231815748E-4 1.501073267386181E-4 20-21 2.0014310231815748E-4 1.501073267386181E-4 0.0 2.0014310231815748E-4 1.751252145283878E-4 22-23 2.0014310231815748E-4 1.501073267386181E-4 0.0 2.7519676568746657E-4 2.0014310231815748E-4 24-25 4.5032198021585434E-4 1.501073267386181E-4 0.0 4.5032198021585434E-4 2.0014310231815748E-4 26-27 4.5032198021585434E-4 1.501073267386181E-4 0.0 8.756260726419389E-4 2.0014310231815748E-4 28-29 5.003577557953937E-4 1.501073267386181E-4 0.0 0.0024267351156076595 2.0014310231815748E-4 30-31 5.003577557953937E-4 1.501073267386181E-4 0.0 0.008080777756095609 2.0014310231815748E-4 32-33 5.003577557953937E-4 1.501073267386181E-4 0.0 0.02048965009982137 2.0014310231815748E-4 34-35 5.003577557953937E-4 1.751252145283878E-4 0.0 0.04005363835142127 2.0014310231815748E-4 36-37 5.003577557953937E-4 2.2516099010792717E-4 0.0 0.07415301940887735 2.0014310231815748E-4 38-39 5.003577557953937E-4 2.5017887789769686E-4 0.0 0.12436392020294511 2.0014310231815748E-4 40-41 5.50393531374933E-4 2.5017887789769686E-4 0.0 0.18305588495774477 2.0014310231815748E-4 42-43 6.004293069544725E-4 2.5017887789769686E-4 0.0 0.2419730107026524 2.0014310231815748E-4 44-45 7.505366336930905E-4 2.7519676568746657E-4 0.0 0.31650129842837627 2.0014310231815748E-4 46-47 7.505366336930905E-4 3.002146534772362E-4 0.0 0.407666481534297 2.0014310231815748E-4 48-49 7.505366336930905E-4 3.002146534772362E-4 0.0 0.504560760944075 2.0014310231815748E-4 50-51 7.505366336930905E-4 3.252325412670059E-4 0.0 0.6164907909155046 2.5017887789769686E-4 52-53 7.505366336930905E-4 4.0028620463631497E-4 0.0 0.7226917245830768 3.002146534772362E-4 54-55 7.755545214828603E-4 4.0028620463631497E-4 0.0 0.8403508508583637 3.002146534772362E-4 56-57 8.005724092726299E-4 4.0028620463631497E-4 0.0 0.9786247166724208 3.002146534772362E-4 58-59 8.005724092726299E-4 4.0028620463631497E-4 0.0 1.1332602811009873 3.002146534772362E-4 60-61 8.255902970623996E-4 5.003577557953937E-4 0.0 1.2963518916024959 3.252325412670059E-4 62-63 9.006439604317087E-4 5.003577557953937E-4 0.0 1.4618202014440325 3.502504290567756E-4 64-65 9.006439604317087E-4 5.003577557953937E-4 0.0 1.6372706485136872 3.502504290567756E-4 66-67 9.006439604317087E-4 5.50393531374933E-4 0.0 1.841741845419475 3.502504290567756E-4 68-69 9.006439604317087E-4 5.50393531374933E-4 0.0 2.0689042665505837 3.502504290567756E-4 70-71 9.256618482214783E-4 5.50393531374933E-4 0.0 2.31152774233577 3.502504290567756E-4 72-73 9.50679736011248E-4 5.50393531374933E-4 0.0 2.5620568706625235 4.2530409242608463E-4 74-75 9.756976238010178E-4 5.50393531374933E-4 0.0 2.8139619828177147 5.003577557953937E-4 76-77 0.0010007155115907874 5.50393531374933E-4 0.0 3.090259535567931 5.003577557953937E-4 78-79 0.0010007155115907874 5.50393531374933E-4 0.0 3.400706505151183 5.003577557953937E-4 80-81 0.001100787062749866 5.50393531374933E-4 0.0 3.7296667116988647 5.503935313749331E-4 82-83 0.001100787062749866 5.754114191647028E-4 0.0 4.081518285574186 6.504650825340118E-4 84-85 0.001125804950539636 6.004293069544725E-4 0.0 4.433119680571608 6.504650825340118E-4 86-87 0.0011508228383294056 6.004293069544725E-4 0.0 4.804310081708421 7.005008581135511E-4 88-89 0.0011508228383294056 6.504650825340118E-4 0.0 5.209499792351531 7.505366336930905E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3480 0.0 47.26423 1 GTATCAA 5970 0.0 41.167255 1 ATCAACG 7735 0.0 31.757656 3 TATCAAC 7850 0.0 31.489761 2 TCAACGC 7830 0.0 31.43303 4 CAACGCA 7960 0.0 30.919676 5 AACGCAG 8315 0.0 29.638184 6 ACGCAGA 9485 0.0 25.731924 7 CGCAGAG 9540 0.0 25.533802 8 TAAGGTG 1325 0.0 25.101542 5 TGGTATC 1705 0.0 23.699013 2 GCAGAGT 10700 0.0 22.366255 9 GTAAGGT 1360 0.0 22.00999 4 GTACATG 6740 0.0 21.723337 1 TACATGG 6675 0.0 21.507572 2 GTGGTAT 2065 0.0 21.40911 1 ACATGGG 6735 0.0 21.023106 3 AGAGTAC 9245 0.0 21.006945 10-11 GAGTACT 6240 0.0 20.545923 12-13 AGGTAAG 1325 0.0 20.091291 2 >>END_MODULE