FastQCFastQC Report
Thu 26 May 2016
SRR938350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975481
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.2814504416898973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.2133657575041218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.1547977429294435No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23840.12067946996199913No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23250.11769285556277179No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21870.11070721510356213No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21470.10868239178205208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.051.8818551
GTATCAA57200.043.6972851
ATCAACG73600.033.511883
TATCAAC75350.032.9858472
TCAACGC75150.032.8206834
CAACGCA76250.032.4078875
AACGCAG80050.030.9819246
ACGCAGA91300.026.7446257
CGCAGAG92150.026.3948298
TGGTATC14250.026.012912
TACATGG64950.025.6093252
GTATCGA950.001210547925.057491
GTACATG67250.024.990421
ACATGGG67750.023.0778753
GCAGAGT103450.022.6839499
GTGGTAT17750.022.2623421
AGAGTAC88700.021.20717210-11
GAGTACT62500.020.9749912-13
CAGAGTA95950.020.29785310-11
GGTAAGG11200.019.9429343
Produced by FastQC (version 0.11.5)