##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938350_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975481 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89070307433987 31.0 31.0 34.0 27.0 34.0 2 30.98838561342782 31.0 31.0 34.0 27.0 34.0 3 31.053452804658715 31.0 31.0 34.0 27.0 34.0 4 34.684830681742824 37.0 35.0 37.0 32.0 37.0 5 34.70239754267442 37.0 35.0 37.0 32.0 37.0 6 34.72259566151231 37.0 35.0 37.0 31.0 37.0 7 34.63740729472974 37.0 35.0 37.0 30.0 37.0 8 34.591171972800545 37.0 35.0 37.0 30.0 37.0 9 36.064183862056886 39.0 35.0 39.0 30.0 39.0 10-11 35.91371620380049 38.0 35.0 39.0 30.0 39.0 12-13 35.75311835446658 38.0 35.0 39.0 30.0 39.0 14-15 36.70043801990502 39.0 36.0 41.0 30.0 41.0 16-17 36.54328312952643 39.0 36.0 41.0 30.0 41.0 18-19 36.533527024557564 39.0 36.0 40.5 29.5 41.0 20-21 36.61590367105531 39.0 36.0 41.0 30.0 41.0 22-23 36.529932203853136 39.0 36.0 41.0 29.5 41.0 24-25 36.40067482299248 39.0 36.0 41.0 29.0 41.0 26-27 36.19091856616186 39.0 35.0 41.0 28.0 41.0 28-29 35.99910604050355 39.0 35.0 40.0 27.0 41.0 30-31 35.75805310200401 38.5 35.0 40.0 27.0 41.0 32-33 35.46294041805515 38.0 34.0 40.0 26.5 41.0 34-35 35.19798773058308 38.0 34.0 40.0 25.5 41.0 36-37 34.884216805932326 38.0 34.0 40.0 25.0 41.0 38-39 34.60727134302988 38.0 33.0 40.0 24.0 41.0 40-41 34.512748540735146 38.0 33.0 40.0 24.0 41.0 42-43 34.31536623232519 38.0 33.0 40.0 23.5 41.0 44-45 34.00967308721268 38.0 33.0 40.0 22.5 41.0 46-47 33.63903525268023 38.0 33.0 40.0 20.5 41.0 48-49 33.24737519621803 37.5 32.0 40.0 18.5 41.0 50-51 32.496192572846816 36.5 30.5 39.5 15.5 40.5 52-53 32.553593782982475 37.0 31.0 39.0 15.0 40.0 54-55 32.654331780462584 37.0 31.0 40.0 14.0 41.0 56-57 32.32067152253046 36.5 31.0 40.0 10.5 41.0 58-59 31.961221089952268 36.0 31.0 39.0 8.0 41.0 60-61 31.485193732564372 36.0 30.0 39.0 7.0 40.5 62-63 30.93394191085614 35.0 29.0 39.0 2.0 40.0 64-65 30.40726992565355 35.0 28.5 38.0 2.0 40.0 66-67 29.84102504655828 34.5 28.0 38.0 2.0 40.0 68-69 29.253063431133988 34.0 27.5 37.5 2.0 39.5 70-71 28.597781755430702 34.0 26.0 36.5 2.0 39.0 72-73 28.017495485909507 33.5 26.0 36.0 2.0 39.0 74-75 27.37709322438434 33.0 25.0 35.5 2.0 38.5 76-77 26.615028694277495 32.5 23.0 35.0 2.0 37.5 78-79 25.812408218555383 32.0 20.5 35.0 2.0 37.0 80-81 25.130058957793064 31.0 18.5 35.0 2.0 36.0 82-83 24.43928946924825 31.0 14.5 34.0 2.0 36.0 84-85 23.748868250314736 30.5 7.5 34.0 2.0 35.0 86-87 23.052500631491775 30.0 2.0 34.0 2.0 35.0 88-89 22.221243585739373 29.0 2.0 34.0 2.0 35.0 90-91 21.355064665263804 29.0 2.0 34.0 2.0 35.0 92-93 20.483235222206645 27.5 2.0 34.0 2.0 35.0 94-95 19.488565822703432 26.5 2.0 33.0 2.0 35.0 96-97 18.235305224398516 24.5 2.0 33.0 2.0 34.5 98-99 16.75931406072749 11.0 2.0 32.0 2.0 34.0 100-101 13.477307298830006 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.737334677112937 1101 2 0.8067805220514579 1101 3 0.809127102780618 1101 4 0.6884340082665474 1101 5 0.6781364395397063 1101 6 0.726662374741899 1101 7 0.662052972856813 1101 8 0.734526541082829 1101 9 0.8062288164922649 1101 10-11 0.8300662796957283 1101 12-13 0.9190866252682213 1101 14-15 1.170138255366787 1101 16-17 1.0147881925257565 1101 18-19 0.858059176214816 1101 20-21 1.0830738301254215 1101 22-23 1.067869991619098 1101 24-25 1.1943033673059844 1101 26-27 1.0911010293549808 1101 28-29 1.20068016900494 1101 30-31 1.2201158928300728 1101 32-33 1.1154200745474512 1101 34-35 1.154286615110614 1101 36-37 1.120761664015241 1101 38-39 1.0494477719791035 1101 40-41 0.9951764402121626 1101 42-43 1.2104756231720373 1101 44-45 1.1436364255987286 1101 46-47 1.4404197225089774 1101 48-49 1.2819557640577486 1101 50-51 1.2477846206785657 1101 52-53 1.4093025882702364 1101 54-55 1.5261345913334452 1101 56-57 1.1948921028187343 1101 58-59 1.3693887794078599 1101 60-61 1.35011127435574 1101 62-63 1.1708444492512733 1101 64-65 1.1804699210047005 1101 66-67 1.2404295727135484 1101 68-69 1.145492242747018 1101 70-71 1.2143215405357424 1101 72-73 1.304722898075692 1101 74-75 1.0498153230046583 1101 76-77 1.464859432613327 1101 78-79 1.5984662745218863 1101 80-81 1.5415351511396587 1101 82-83 1.5103176159806573 1101 84-85 1.7098098434299231 1101 86-87 1.6500785441763952 1101 88-89 1.7826418013815264 1101 90-91 1.9453801876888939 1101 92-93 1.8709272066910145 1101 94-95 1.7926457249375183 1101 96-97 1.8664135858117739 1101 98-99 1.808142160386943 1101 100-101 1.5763741404353109 1102 1 0.5896990529315076 1102 2 0.5923516465451719 1102 3 0.5737971366827459 1102 4 0.4377781075623943 1102 5 0.625336432855363 1102 6 0.5169846016292325 1102 7 0.4128524823934825 1102 8 0.46387715760860004 1102 9 0.5299980104654978 1102 10-11 0.6283517468996536 1102 12-13 0.7116872715601872 1102 14-15 0.6283407741090414 1102 16-17 0.6679574566845403 1102 18-19 0.7119552055089287 1102 20-21 0.6075249310568438 1102 22-23 0.7575955368626666 1102 24-25 0.6629290151123755 1102 26-27 0.5604240938734719 1102 28-29 0.5152709830529147 1102 30-31 0.49536337001455166 1102 32-33 0.7575915132050781 1102 34-35 0.6937150728457553 1102 36-37 0.6317359898892647 1102 38-39 0.7103656465266042 1102 40-41 0.8295223463682362 1102 42-43 0.9374788880535689 1102 44-45 0.9896501577364063 1102 46-47 0.909561839898501 1102 48-49 0.8880612805168866 1102 50-51 0.9665692826285976 1102 52-53 0.834869347055438 1102 54-55 0.7811823112429437 1102 56-57 0.9050505738598886 1102 58-59 1.0844531892747717 1102 60-61 0.917940859237433 1102 62-63 0.9168610013441985 1102 64-65 0.7697593653899624 1102 66-67 0.7747648779267067 1102 68-69 0.8514173865125159 1102 70-71 0.9188975445964793 1102 72-73 0.9883393235839648 1102 74-75 0.9314022684917909 1102 76-77 1.0211846444755182 1102 78-79 1.1126728435554405 1102 80-81 1.1066238961760142 1102 82-83 0.9619624332162218 1102 84-85 1.0786456301243348 1102 86-87 0.8956997803194469 1102 88-89 1.2261575753756908 1102 90-91 1.4548962311454474 1102 92-93 1.5448680523851515 1102 94-95 1.4453037416525412 1102 96-97 1.4143703193427122 1102 98-99 1.4805565747448988 1102 100-101 1.3954818439264134 1103 1 0.6191345983642407 1103 2 0.6591552517094712 1103 3 0.6256226717399365 1103 4 0.5945062173956899 1103 5 0.6502148915001484 1103 6 0.7145818684676826 1103 7 0.5568721076483314 1103 8 0.5433869105169222 1103 9 0.7456351198116948 1103 10-11 0.7229281099751788 1103 12-13 0.7137551103830049 1103 14-15 0.8336259665759158 1103 16-17 0.913698835583034 1103 18-19 0.8689969704524643 1103 20-21 0.8820606539851354 1103 22-23 0.9378205168957194 1103 24-25 0.9768200158071991 1103 26-27 1.0155664797918078 1103 28-29 0.8338096877819368 1103 30-31 0.8423290164712327 1103 32-33 1.0869892278848639 1103 34-35 1.1428994397349896 1103 36-37 1.0472158043134527 1103 38-39 0.9514769774732486 1103 40-41 0.9947227792538271 1103 42-43 0.9430296487710663 1103 44-45 0.9680824741832552 1103 46-47 1.1365695961116202 1103 48-49 1.2109892094272752 1103 50-51 1.1630028059206055 1103 52-53 1.3930521103569617 1103 54-55 1.6167626407054456 1103 56-57 1.4684396744486605 1103 58-59 1.45208917486325 1103 60-61 1.4150118969269414 1103 62-63 1.580622982813619 1103 64-65 1.2493750288276395 1103 66-67 1.4809683165006753 1103 68-69 1.4840688315589965 1103 70-71 1.5230677814268603 1103 72-73 1.4910634234093756 1103 74-75 1.4709111282100586 1103 76-77 1.3529007585587358 1103 78-79 1.3296322117169517 1103 80-81 1.4840314847602123 1103 82-83 1.66900479535078 1103 84-85 1.563448546701423 1103 86-87 1.5173242229844384 1103 88-89 1.7125083851147842 1103 90-91 1.6970949690471393 1103 92-93 1.7373540132031877 1103 94-95 1.8971175665727529 1103 96-97 1.9214321031678736 1103 98-99 2.1520556851899286 1103 100-101 1.4161604918028576 1104 1 0.48067320252548384 1104 2 0.5978951628581761 1104 3 0.5952823924779764 1104 4 0.6042018875549857 1104 5 0.5553194938428732 1104 6 0.5451011932860368 1104 7 0.5636550929586264 1104 8 0.3926230855365844 1104 9 0.577125339579716 1104 10-11 0.4337170454308321 1104 12-13 0.48900432972082797 1104 14-15 0.767734520899559 1104 16-17 0.492874880259734 1104 18-19 0.7525525907065145 1104 20-21 0.6818606120424704 1104 22-23 0.8031639017687482 1104 24-25 0.7122875975621881 1104 26-27 0.7304157605653572 1104 28-29 0.7659404977739186 1104 30-31 0.6934754116447692 1104 32-33 0.7518221349848702 1104 34-35 0.6769994000723685 1104 36-37 0.7787195038616801 1104 38-39 0.7079036130178267 1104 40-41 0.6306818969345613 1104 42-43 0.7935371324293428 1104 44-45 0.9226454674761868 1104 46-47 1.0216242781679554 1104 48-49 1.0816782792547883 1104 50-51 0.8956501570811568 1104 52-53 1.0169266551951424 1104 54-55 1.2938305780460126 1104 56-57 1.212201455162706 1104 58-59 1.2181916868378302 1104 60-61 1.380562163854151 1104 62-63 1.4026209818930901 1104 64-65 1.2950825080761739 1104 66-67 1.2073683658096144 1104 68-69 1.2149344965293345 1104 70-71 1.2703413204930385 1104 72-73 1.187528213246324 1104 74-75 1.076702222184423 1104 76-77 1.2090933019351944 1104 78-79 1.1612745174809582 1104 80-81 1.0324978089870136 1104 82-83 0.967078564825993 1104 84-85 0.8195648716924069 1104 86-87 0.8836689450616859 1104 88-89 1.018171421177204 1104 90-91 1.0077079407548801 1104 92-93 1.038107202185948 1104 94-95 0.8532435128194962 1104 96-97 0.5918865546648426 1104 98-99 0.6027304634437876 1104 100-101 0.4545063323510856 1105 1 0.39982892772072987 1105 2 0.44950731690717305 1105 3 0.5193388197930453 1105 4 0.5033941756808815 1105 5 0.3784338150292754 1105 6 0.47232369920274664 1105 7 0.38354395203555214 1105 8 0.28439940733653657 1105 9 0.43948927441268637 1105 10-11 0.5508364692713599 1105 12-13 0.5290463645892842 1105 14-15 0.6845271671536892 1105 16-17 0.6994096386049193 1105 18-19 0.7969627994768018 1105 20-21 0.8236495558430761 1105 22-23 0.7580021430167747 1105 24-25 0.8194529510599509 1105 26-27 0.7851517275452409 1105 28-29 0.8856398585477123 1105 30-31 0.8224301849728661 1105 32-33 0.836860279872937 1105 34-35 0.9083134296785289 1105 36-37 0.8873836297797197 1105 38-39 0.911288881676505 1105 40-41 0.9188665743546949 1105 42-43 0.9291090797564081 1105 44-45 0.9602863169825042 1105 46-47 0.9213295003584392 1105 48-49 1.063635910684674 1105 50-51 0.990079190663856 1105 52-53 0.7671469992636659 1105 54-55 0.7449226095005201 1105 56-57 0.7539620054723102 1105 58-59 0.9313764363836832 1105 60-61 0.9482061099579653 1105 62-63 0.9504284321912557 1105 64-65 0.9850229418498557 1105 66-67 0.8398383062145349 1105 68-69 0.800652907147775 1105 70-71 0.9352858654326539 1105 72-73 0.9474006613182127 1105 74-75 0.9593908237244513 1105 76-77 0.8588217245649723 1105 78-79 1.049227334759589 1105 80-81 1.122315604998171 1105 82-83 1.0867740788948375 1105 84-85 0.836363672674473 1105 86-87 1.0394784071865466 1105 88-89 1.0286008000016729 1105 90-91 1.0872764318175463 1105 92-93 1.3861660827371054 1105 94-95 1.22751083573403 1105 96-97 1.2500595765689404 1105 98-99 1.3808020333129285 1105 100-101 1.0577535764274 1106 1 0.2234487848475446 1106 2 0.29394701042950544 1106 3 0.13514758510036984 1106 4 0.21616782159610182 1106 5 0.37464243521752394 1106 6 -0.10778620546208373 1106 7 0.25063978533289344 1106 8 0.289298615804114 1106 9 0.31150807078816456 1106 10-11 0.08792317503955616 1106 12-13 0.009470405982774821 1106 14-15 0.38079279840727054 1106 16-17 0.0366412474974851 1106 18-19 -0.2797070056777997 1106 20-21 0.06096063470550206 1106 22-23 0.20486379904426144 1106 24-25 0.12521941876375564 1106 26-27 0.07086426508472954 1106 28-29 0.05819047026492541 1106 30-31 0.24681781990626206 1106 32-33 0.460724017668106 1106 34-35 0.44025150356832654 1106 36-37 0.17849134326169036 1106 38-39 0.3966556816569309 1106 40-41 0.4209314258811787 1106 42-43 -0.019790793840265053 1106 44-45 0.3917632634928836 1106 46-47 0.30689814254490244 1106 48-49 0.47321758479445464 1106 50-51 0.1302749430515533 1106 52-53 0.4643674938778446 1106 54-55 0.31533847354928923 1106 56-57 -0.25097391466612606 1106 58-59 -0.4535105204635492 1106 60-61 0.35128046044932404 1106 62-63 0.2123975166453711 1106 64-65 0.5411392501818213 1106 66-67 -0.09282241172178018 1106 68-69 -0.33447807433514143 1106 70-71 -0.2001290916218892 1106 72-73 0.268848411252705 1106 74-75 0.1114753987760615 1106 76-77 0.11292335398728781 1106 78-79 0.46244274444743994 1106 80-81 0.36233974832053306 1106 82-83 0.1735475117383949 1106 84-85 0.04614063847646932 1106 86-87 0.5900674495446161 1106 88-89 0.48076333336610233 1106 90-91 0.6670008639383838 1106 92-93 0.2901380695727305 1106 94-95 0.14795520531545847 1106 96-97 0.21226635109593772 1106 98-99 -0.040629232361514056 1106 100-101 -0.4405512081830665 1107 1 0.5471973487733592 1107 2 0.4763767955683633 1107 3 0.47672756351997236 1107 4 0.43534224917431175 1107 5 0.410159998099239 1107 6 0.34873488082109816 1107 7 0.4335605419737547 1107 8 0.299127124975783 1107 9 0.38217933289222117 1107 10-11 0.5392670611174069 1107 12-13 0.36487440987981046 1107 14-15 0.5907463202373435 1107 16-17 0.5099286962964271 1107 18-19 0.49868815304986924 1107 20-21 0.621079152292829 1107 22-23 0.6425278781584112 1107 24-25 0.5014694580484544 1107 26-27 0.6750588238913124 1107 28-29 0.8046228953359034 1107 30-31 0.8821021189449212 1107 32-33 0.7674977482483669 1107 34-35 0.8635859194135023 1107 36-37 0.810684197985104 1107 38-39 0.7342960153499334 1107 40-41 1.0701284465292247 1107 42-43 1.0596750040233403 1107 44-45 1.0531993027566386 1107 46-47 1.0394052749011848 1107 48-49 0.8118244968071977 1107 50-51 0.8448249498609002 1107 52-53 1.0611590684437147 1107 54-55 0.9543581368531875 1107 56-57 1.0766922945519823 1107 58-59 0.7846228799156769 1107 60-61 0.6413824610010579 1107 62-63 0.72173924468348 1107 64-65 0.797828300399896 1107 66-67 0.8042362988320129 1107 68-69 0.6672501034482217 1107 70-71 0.7828429970323398 1107 72-73 0.7783315642255957 1107 74-75 0.618184527842903 1107 76-77 0.6071710257138285 1107 78-79 0.7675623270867433 1107 80-81 0.5492815126751651 1107 82-83 0.6632702839464706 1107 84-85 0.6700889432978379 1107 86-87 0.6736443480434176 1107 88-89 0.5110942677135064 1107 90-91 0.5531104909606732 1107 92-93 0.6219101414654844 1107 94-95 0.6699375289445975 1107 96-97 0.8126005329788804 1107 98-99 0.5171049655845081 1107 100-101 0.4084254689246034 1108 1 0.37401173541433863 1108 2 0.39297991941536736 1108 3 0.31202662472605525 1108 4 0.3592883266008968 1108 5 0.37386474946233506 1108 6 0.4307344981976655 1108 7 0.4466066344319728 1108 8 0.28848677733506634 1108 9 0.15070855575670095 1108 10-11 0.42094486485891736 1108 12-13 0.5880244939203223 1108 14-15 0.5531327289064905 1108 16-17 0.4597083461959812 1108 18-19 0.5562685381330184 1108 20-21 0.5494732270957101 1108 22-23 0.589986165371954 1108 24-25 0.5431596970514505 1108 26-27 0.5107739880777515 1108 28-29 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-1.0198719259935416 2316 70-71 -1.404776016898559 2316 72-73 -1.195208580683996 2316 74-75 -0.9016936726369842 2316 76-77 -0.9458860479141613 2316 78-79 -0.9546604846781683 2316 80-81 -0.9683747013995792 2316 82-83 -0.8161440643435576 2316 84-85 -0.9989960274903211 2316 86-87 -0.9547721992408533 2316 88-89 -1.1587028706462696 2316 90-91 -1.185499839006372 2316 92-93 -1.2952148946440047 2316 94-95 -1.4041514802109987 2316 96-97 -1.471805473282064 2316 98-99 -1.247569046784097 2316 100-101 -0.5937528913213566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7718.0 3 2878.0 4 3088.0 5 3382.0 6 3962.0 7 5083.0 8 6430.0 9 8038.0 10 9965.0 11 12202.0 12 14729.0 13 16420.0 14 19096.0 15 21188.0 16 24056.0 17 27367.0 18 26643.0 19 25833.0 20 27197.0 21 29318.0 22 32201.0 23 35606.0 24 39074.0 25 43490.0 26 48837.0 27 55024.0 28 62319.0 29 70883.0 30 80853.0 31 93679.0 32 110083.0 33 131142.0 34 158061.0 35 190621.0 36 220262.0 37 209185.0 38 94825.0 39 4743.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.807432855638034 18.277385826563997 14.609594783135261 23.3055865346627 2 15.511598626053328 19.43166175223783 40.27277359153752 24.783966030171317 3 19.443015126683697 23.557221892466234 29.17857671013368 27.821186270716385 4 13.070716269335788 16.11840255917925 36.44824737652218 34.36263379496278 5 14.103108929276026 37.005471865453806 33.98999433211382 14.901424873156355 6 33.116908443559254 35.42968985458942 17.06982513956265 14.383576562288681 7 29.339578070096817 32.03896326836924 20.154366327844627 18.467092333689322 8 26.675271696473697 34.78864338616194 19.353670776083217 19.182414141281154 9 26.259224148582984 16.936985113748463 19.79700289865641 37.00678783901215 10-11 25.20649762655283 26.25834985727919 27.116978901932203 21.418173614235773 12-13 26.33430782093398 23.419985587779085 27.28669938365727 22.959007207629668 14-15 22.864796367259252 25.005728377432344 25.528536181191424 26.600939074116976 16-17 22.5313400155912 28.1397931924825 26.439825372443167 22.88904141948313 18-19 22.646546306419815 26.585836815326815 28.6032492303828 22.16436764787057 20-21 23.04143719440981 25.855525921544036 28.895499423132833 22.207537460913322 22-23 22.679285655501605 25.758968872457473 28.859112576995877 22.702632895045042 24-25 22.657283287971556 25.941668452264267 28.85561176599908 22.5454364937651 26-27 22.194332344694576 26.303235563443057 28.654671318130987 22.84776077373138 28-29 22.349444389861976 26.74223862622342 28.49944241476441 22.408874569150193 30-31 22.442954549771134 26.236817281089948 28.40933695466373 22.910891214475193 32-33 22.13969158041424 26.229896345149157 28.39461216605541 23.235799908381196 34-35 22.95799685489201 26.361829227220124 28.213467240990735 22.46670667689713 36-37 22.993205788563817 25.927952643351126 28.24260837350919 22.836233194575875 38-39 22.457210244123008 26.300326034065723 28.240783170383814 23.001680551427455 40-41 22.954449941011763 26.11153587143096 28.351443313062987 22.582570874494294 42-43 22.666831727534127 26.130806180567472 28.162700395791656 23.03966169610674 44-45 22.59672103595683 26.448452106536845 28.270453153914087 22.68437370359224 46-47 23.073751164713897 26.685889350344677 27.515573183520942 22.724786301420487 48-49 22.792140269449863 26.704691578527438 27.504044987961223 22.999123164061473 50-51 22.9177914886885 26.91748773928176 27.475095712703684 22.689625059326058 52-53 23.240807608610083 26.551083550580078 27.515955264256338 22.6921535765535 54-55 22.68466497693088 26.817640922623077 27.682919636635084 22.81477446381096 56-57 22.757969896340217 26.893021240231903 27.52628957104411 22.82271929238377 58-59 22.752240722215248 26.47973224736439 27.547846962621552 23.220180067798807 60-61 22.384989612683587 26.800769828358046 27.81107462338226 23.00316593557611 62-63 22.588626336396743 26.736641854152637 27.898296787838706 22.77643502161192 64-65 22.3061087547115 26.86235653517206 27.7175199274459 23.114014782670544 66-67 22.22706230964303 26.865083814649232 27.64857050506973 23.259283370638002 68-69 22.844257288578262 26.407615655246204 27.843853552334725 22.904273503840813 70-71 22.582654121661903 26.53863436998819 27.474724971487667 23.403986536862238 72-73 22.635559340501327 26.688061623957758 27.417147492045874 23.259231543495034 74-75 22.62821304742308 27.005471192948917 27.353888870886735 23.01242688874127 76-77 22.801469408171354 26.709596970864467 27.3751694117659 23.11376420919828 78-79 22.84057549892452 26.945189300996912 27.276869128717795 22.937366071360774 80-81 22.995181652412768 26.902381634593286 27.051518554029986 23.05091815896396 82-83 22.88974261752254 27.011390679395987 27.19474715587376 22.904119547207713 84-85 22.803356705945006 27.09066416791832 27.176495363697985 22.92948376243869 86-87 23.070550174263744 27.00985490635523 26.88460877416487 23.03498614521616 88-89 23.019268045183598 27.10582841656894 26.637393942708304 23.237509595539162 90-91 22.872291267617758 27.13734287548155 26.76944773952113 23.22091811737956 92-93 23.032296414513688 26.75727512356849 26.98107055226838 23.22935790964943 94-95 22.836585629537247 26.580897497538402 27.16046475769754 23.422052115226812 96-97 23.196440887560122 26.205107248367142 27.114229461575622 23.48422240249711 98-99 22.98936474543489 26.474644753130956 26.68013478242158 23.855855719012574 100-101 23.515415773799266 25.903325014546052 27.028236572524484 23.553022639130198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 187.0 2 185.5 3 248.0 4 228.5 5 237.5 6 399.0 7 883.0 8 1424.0 9 1589.0 10 1415.0 11 1213.0 12 1217.5 13 1334.5 14 1449.5 15 1564.5 16 1735.0 17 1904.5 18 2139.0 19 2489.5 20 2867.0 21 3358.5 22 4046.0 23 4990.5 24 6215.5 25 7759.0 26 9847.0 27 12389.0 28 14910.5 29 17808.5 30 20976.0 31 25058.5 32 31295.0 33 34509.5 34 35081.5 35 37699.0 36 41625.0 37 45602.5 38 49603.0 39 53133.5 40 56078.5 41 59782.5 42 62380.5 43 64222.5 44 66414.0 45 69689.5 46 72199.0 47 73895.5 48 74743.0 49 73781.0 50 72292.5 51 70483.0 52 68834.5 53 68426.5 54 68898.5 55 65811.5 56 61138.5 57 56869.5 58 53401.5 59 47861.0 60 39257.5 61 32807.0 62 27738.0 63 21442.0 64 15609.0 65 11977.5 66 9191.0 67 6992.5 68 5369.0 69 4195.5 70 3323.5 71 2469.5 72 1825.0 73 1471.5 74 1127.5 75 822.5 76 617.5 77 429.5 78 295.0 79 215.5 80 139.0 81 77.5 82 49.0 83 30.0 84 20.0 85 14.5 86 12.0 87 8.5 88 2.5 89 2.0 90 2.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1884098100665104 2 0.05178485644761959 3 0.048443897967128 4 0.055581400175450935 5 0.060744699645301575 6 0.06469310512224619 7 0.035333166960350416 8 0.03376392888618013 9 0.03912971068818177 10-11 0.041584808965512705 12-13 0.03887660777299301 14-15 0.033586756845547995 16-17 0.03287806868301948 18-19 0.04039522526412555 20-21 0.05178485644761959 22-23 0.047760520096118364 24-25 0.06843902826703978 26-27 0.05310099160660113 28-29 0.045305421818787425 30-31 0.05408809297583728 32-33 0.050392790414081436 34-35 0.049911894875222795 36-37 0.0472036936827031 38-39 0.0270313913421592 40-41 0.02586711793229092 42-43 0.022880503533063593 44-45 0.003568751104161468 46-47 0.01065563272944665 48-49 0.009972254858437009 50-51 0.008529568241861097 52-53 0.009744462234767128 54-55 0.011212459142861916 56-57 0.008757360865530977 58-59 0.011389631183494045 60-61 0.011921147305390433 62-63 0.0033662687720104625 64-65 0.006454124337313291 66-67 0.004074956934538981 68-69 0.009187635821351863 70-71 0.023209537322808976 72-73 0.02050133613028928 74-75 0.020703818462440286 76-77 0.013363833921966347 78-79 0.004150887809095608 80-81 0.005821367049341401 82-83 0.004758334805548624 84-85 0.0046570936394731205 86-87 0.0086308094079366 88-89 0.030625452737839544 90-91 0.03713019765819059 92-93 0.023437329946478857 94-95 0.03267558635086847 96-97 0.03639619920414319 98-99 0.022576780034837086 100-101 0.12336236086299995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.77075820163624 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58656653094897 48.97306748952372 2 11.514371728860546 13.994742003566813 3 3.4405781966658653 6.272596369902088 4 1.4826575181826336 3.6040888613325968 5 0.7773981166544381 2.3621536486807133 6 0.48427645481396736 1.765790840294714 7 0.3180284002779379 1.3528778910180623 8 0.2330843175273191 1.1331768560836892 9 0.16031783336869532 0.8768372658353273 >10 0.9040394223629644 10.003892210014783 >50 0.0627231295739684 2.5774560798410735 >100 0.030691858771464305 3.3644052591274622 >500 0.0029258288840509484 1.1990650372304872 >1k 0.0022570679962678683 2.2373944994155406 >5k 8.359511097288401E-5 0.2824556881329278 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.2814504416898973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4215 0.2133657575041218 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3058 0.1547977429294435 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2384 0.12067946996199913 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2325 0.11769285556277179 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2187 0.11070721510356213 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2147 0.10868239178205208 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.062058303775131E-5 0.0 0.0 0.0 0.0 3 5.062058303775131E-5 0.0 0.0 0.0 0.0 4 5.062058303775131E-5 0.0 0.0 0.0 0.0 5 5.062058303775131E-5 5.062058303775131E-5 0.0 0.0 5.062058303775131E-5 6 5.062058303775131E-5 5.062058303775131E-5 0.0 0.0 1.0124116607550262E-4 7 5.062058303775131E-5 5.062058303775131E-5 0.0 0.0 1.0124116607550262E-4 8 1.0124116607550262E-4 5.062058303775131E-5 0.0 0.0 1.0124116607550262E-4 9 1.0124116607550262E-4 5.062058303775131E-5 0.0 5.062058303775131E-5 1.0124116607550262E-4 10-11 1.0124116607550262E-4 5.062058303775131E-5 0.0 5.062058303775131E-5 1.2655145759437827E-4 12-13 1.0124116607550262E-4 5.062058303775131E-5 0.0 5.062058303775131E-5 2.784132067076322E-4 14-15 1.0124116607550262E-4 5.062058303775131E-5 0.0 1.2655145759437827E-4 3.037234982265079E-4 16-17 1.0124116607550262E-4 5.062058303775131E-5 0.0 1.5186174911325395E-4 3.037234982265079E-4 18-19 1.2655145759437827E-4 5.062058303775131E-5 0.0 1.5186174911325395E-4 3.037234982265079E-4 20-21 1.5186174911325395E-4 5.062058303775131E-5 0.0 1.771720406321296E-4 3.037234982265079E-4 22-23 1.5186174911325395E-4 5.062058303775131E-5 0.0 2.784132067076322E-4 3.037234982265079E-4 24-25 2.0248233215100524E-4 7.593087455662697E-5 0.0 4.302749558208861E-4 3.037234982265079E-4 26-27 2.0248233215100524E-4 1.0124116607550262E-4 0.0 6.833778710096427E-4 3.037234982265079E-4 28-29 3.290337897453835E-4 1.0124116607550262E-4 0.0 0.002429787985812063 3.037234982265079E-4 30-31 3.543440812642592E-4 1.0124116607550262E-4 0.0 0.009390118153502868 3.037234982265079E-4 32-33 3.543440812642592E-4 1.0124116607550262E-4 0.0 0.02348795052951661 4.049646643020105E-4 34-35 4.049646643020105E-4 1.0124116607550262E-4 0.0 0.04505231890359867 4.049646643020105E-4 36-37 4.555852473397618E-4 1.5186174911325395E-4 0.0 0.08061327848761897 4.049646643020105E-4 38-39 4.555852473397618E-4 2.0248233215100524E-4 0.0 0.13409392446700324 4.049646643020105E-4 40-41 4.555852473397618E-4 2.277926236698809E-4 0.0 0.19337062720421 4.049646643020105E-4 42-43 5.315161218963887E-4 2.5310291518875654E-4 0.0 0.2544696709307758 4.049646643020105E-4 44-45 5.568264134152644E-4 2.5310291518875654E-4 0.0 0.3281479295422229 4.049646643020105E-4 46-47 6.074469964530158E-4 2.5310291518875654E-4 0.0 0.4201002186302981 4.049646643020105E-4 48-49 6.58067579490767E-4 2.5310291518875654E-4 0.0 0.5187344246793566 4.049646643020105E-4 50-51 7.086881625285184E-4 2.5310291518875654E-4 0.0 0.6270371620886255 4.049646643020105E-4 52-53 7.086881625285184E-4 2.5310291518875654E-4 0.0 0.7336441099661297 4.555852473397618E-4 54-55 7.086881625285184E-4 2.5310291518875654E-4 0.0 0.8550069577991386 4.555852473397618E-4 56-57 7.086881625285184E-4 2.5310291518875654E-4 0.0 0.9961877638914269 4.555852473397618E-4 58-59 7.086881625285184E-4 2.5310291518875654E-4 0.0 1.1476951689234167 4.555852473397618E-4 60-61 7.086881625285184E-4 3.037234982265079E-4 0.0 1.3103897228067494 4.555852473397618E-4 62-63 7.086881625285184E-4 3.037234982265079E-4 0.0 1.4752609617607053 4.555852473397618E-4 64-65 7.086881625285184E-4 3.037234982265079E-4 0.0 1.6467888073841257 5.062058303775131E-4 66-67 7.593087455662697E-4 3.543440812642592E-4 0.0 1.8497773453655086 5.062058303775131E-4 68-69 7.593087455662697E-4 3.543440812642592E-4 0.0 2.073596253266926 5.062058303775131E-4 70-71 8.352396201228966E-4 3.7965437278313487E-4 0.0 2.3105258921751206 5.062058303775131E-4 72-73 8.605499116417723E-4 4.302749558208861E-4 0.0 2.5577821300230172 5.315161218963887E-4 74-75 8.605499116417723E-4 4.555852473397618E-4 0.0 2.8128086273672084 5.568264134152644E-4 76-77 8.605499116417723E-4 4.555852473397618E-4 0.0 3.092942933898124 5.568264134152644E-4 78-79 8.605499116417723E-4 4.555852473397618E-4 0.0 3.409827783714447 5.568264134152644E-4 80-81 9.364807861983993E-4 4.555852473397618E-4 0.0 3.7346347547761787 5.568264134152644E-4 82-83 9.617910777172749E-4 4.555852473397618E-4 0.0 4.080702370713766 6.074469964530158E-4 84-85 9.871013692361505E-4 4.555852473397618E-4 0.0 4.425074197119588 6.074469964530158E-4 86-87 0.0010377219522739018 5.062058303775131E-4 0.0 4.781848066369658 6.327572879718914E-4 88-89 0.0010630322437927776 5.062058303775131E-4 0.0 5.1675515988258045 6.58067579490767E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3120 0.0 51.881855 1 GTATCAA 5720 0.0 43.697285 1 ATCAACG 7360 0.0 33.51188 3 TATCAAC 7535 0.0 32.985847 2 TCAACGC 7515 0.0 32.820683 4 CAACGCA 7625 0.0 32.407887 5 AACGCAG 8005 0.0 30.981924 6 ACGCAGA 9130 0.0 26.744625 7 CGCAGAG 9215 0.0 26.394829 8 TGGTATC 1425 0.0 26.01291 2 TACATGG 6495 0.0 25.609325 2 GTATCGA 95 0.0012105479 25.05749 1 GTACATG 6725 0.0 24.99042 1 ACATGGG 6775 0.0 23.077875 3 GCAGAGT 10345 0.0 22.683949 9 GTGGTAT 1775 0.0 22.262342 1 AGAGTAC 8870 0.0 21.207172 10-11 GAGTACT 6250 0.0 20.97499 12-13 CAGAGTA 9595 0.0 20.297853 10-11 GGTAAGG 1120 0.0 19.942934 3 >>END_MODULE