FastQCFastQC Report
Thu 26 May 2016
SRR938350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975481
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.28393085025874715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.23001992932354196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.16871840326482512No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24350.12326111969692445No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23470.11880650838960233No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22970.11627547923771477No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22700.11490872349569548No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21480.10873301236508982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.048.6502761
GTATCAA61450.037.9280781
ATCAACG77900.029.6603533
TCAACGC79600.029.086634
TATCAAC80350.028.8882052
CAACGCA81750.028.2635085
AACGCAG85450.027.068586
ACGCAGA96400.023.9938817
CGCAGAG98500.023.4823388
TAAGGTG12500.022.8201625
GGACCGA6150.022.3960516
TACATGG63900.021.7354492
GAGTACT63950.021.612312-13
TAGCGAT1100.002879822221.588677
GTACATG67000.021.4397031
TGGTATC19750.021.434312
ACATGGG64100.020.8413473
GCAGAGT110500.020.6743289
GTAAGGT12700.020.589064
AGAGTAC93650.020.23548710-11
Produced by FastQC (version 0.11.5)