##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938350_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975481 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81487749059596 31.0 31.0 34.0 30.0 34.0 2 32.05006578144766 33.0 31.0 34.0 30.0 34.0 3 32.11118659202493 33.0 31.0 34.0 30.0 34.0 4 35.773564514161365 37.0 35.0 37.0 35.0 37.0 5 35.639536902658136 37.0 35.0 37.0 33.0 37.0 6 35.670564789031125 37.0 35.0 37.0 33.0 37.0 7 35.61432633368785 37.0 35.0 37.0 33.0 37.0 8 35.60089466818461 37.0 35.0 37.0 33.0 37.0 9 37.18725059871495 39.0 37.0 39.0 33.0 39.0 10-11 37.102586154966815 39.0 37.0 39.0 33.0 39.0 12-13 36.99561625750893 39.0 37.0 39.0 33.0 39.0 14-15 38.06172319551543 40.0 37.0 41.0 33.0 41.0 16-17 37.97950246041344 40.0 37.0 41.0 33.0 41.0 18-19 38.08955363276083 40.0 37.5 41.0 33.0 41.0 20-21 38.143036303563534 40.0 37.0 41.0 33.0 41.0 22-23 38.04834569403603 40.0 37.0 41.0 33.0 41.0 24-25 37.94495669662224 40.0 37.0 41.0 33.0 41.0 26-27 37.75392296863397 40.0 37.0 41.0 32.0 41.0 28-29 37.63469605630224 40.0 37.0 41.0 32.0 41.0 30-31 37.39538674378544 39.0 36.0 41.0 31.5 41.0 32-33 37.18539459503786 39.0 36.0 41.0 31.0 41.0 34-35 37.03160723894585 39.0 36.0 41.0 31.0 41.0 36-37 37.01098086997547 39.0 36.0 41.0 31.0 41.0 38-39 36.887676975885874 39.0 35.5 41.0 30.0 41.0 40-41 36.71525036181062 39.0 35.0 41.0 30.0 41.0 42-43 36.50135485990501 39.0 35.0 40.5 30.0 41.0 44-45 36.240497630703615 39.0 35.0 40.0 30.0 41.0 46-47 35.88625580301709 38.5 35.0 40.0 28.5 41.0 48-49 35.52466209495307 38.0 34.5 40.0 27.5 41.0 50-51 35.161148095071525 38.0 34.0 40.0 26.5 41.0 52-53 34.66647211489253 38.0 33.0 40.0 26.0 41.0 54-55 34.34797854294726 38.0 33.0 40.0 24.5 41.0 56-57 34.00220756362628 37.0 33.0 40.0 24.0 41.0 58-59 33.54943302415968 37.0 32.0 39.5 23.0 41.0 60-61 33.17753777434457 36.0 32.0 39.0 22.0 40.5 62-63 32.71527820313129 36.0 31.0 39.0 21.5 40.0 64-65 32.205610937285655 35.0 31.0 38.5 19.5 40.0 66-67 31.70721965941459 35.0 30.0 38.0 18.0 40.0 68-69 31.166272922898273 34.5 29.5 37.5 15.5 40.0 70-71 30.584080788425705 34.0 29.0 37.0 12.0 39.0 72-73 30.135493077382165 34.0 29.0 36.0 8.0 39.0 74-75 29.614202313259405 34.0 28.0 36.0 7.0 39.0 76-77 28.35009296470075 31.5 26.5 34.5 7.0 36.5 78-79 29.035827983159542 33.5 28.0 35.0 4.0 37.0 80-81 28.76709216641415 33.0 28.0 35.0 2.0 37.0 82-83 28.293596344383975 33.0 27.0 35.0 2.0 36.0 84-85 27.771147634424224 33.0 26.0 35.0 2.0 36.0 86-87 27.199318039505314 32.5 25.5 35.0 2.0 35.5 88-89 26.542287928863907 32.0 25.0 34.0 2.0 35.0 90-91 25.988041393463163 32.0 23.5 34.0 2.0 35.0 92-93 25.248107169848762 31.0 20.5 34.0 2.0 35.0 94-95 24.472923050133105 31.0 17.5 34.0 2.0 35.0 96-97 23.469442631946347 31.0 4.5 34.0 2.0 35.0 98-99 22.216474620611386 30.0 2.0 34.0 2.0 35.0 100-101 18.540255259352026 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37060530462999 1101 2 0.3697744753493737 1101 3 0.3482599440963057 1101 4 0.283353044528873 1101 5 0.15757065865714992 1101 6 0.24676164599123496 1101 7 0.3125817743510737 1101 8 0.25137257859882567 1101 9 0.2999589345466944 1101 10-11 0.3963526304832854 1101 12-13 0.3589179571047225 1101 14-15 0.5618370880861718 1101 16-17 0.5753443347685376 1101 18-19 0.581309689380852 1101 20-21 0.5442129819136952 1101 22-23 0.5336478600097365 1101 24-25 0.5837668041845561 1101 26-27 0.5910596564114741 1101 28-29 0.5176334778161475 1101 30-31 0.6134332418911796 1101 32-33 0.5858037210761324 1101 34-35 0.5665480478021152 1101 36-37 0.6631541547213899 1101 38-39 0.6878110809803957 1101 40-41 0.699338012987937 1101 42-43 0.8597100284394728 1101 44-45 0.8088062808182812 1101 46-47 0.8083037908345503 1101 48-49 1.0291020449575612 1101 50-51 1.0999177348869367 1101 52-53 0.9669229712663636 1101 54-55 1.0287236636743202 1101 56-57 1.077451962250656 1101 58-59 1.2033855476940403 1101 60-61 1.0716797219657437 1101 62-63 1.050040861170693 1101 64-65 1.0625380706366059 1101 66-67 1.0147001175865462 1101 68-69 0.8648075615097461 1101 70-71 0.7308021233392168 1101 72-73 0.7345925822387755 1101 74-75 0.9076498551720356 1101 76-77 0.9409788201456522 1101 78-79 0.9189789717473076 1101 80-81 0.8987531220365348 1101 82-83 0.7256186046348283 1101 84-85 0.9790320060328561 1101 86-87 0.8400041997835466 1101 88-89 0.8948898958260223 1101 90-91 1.0210858938254255 1101 92-93 0.7529695266914871 1101 94-95 0.8489863641390301 1101 96-97 0.921281653775246 1101 98-99 0.820575366701334 1101 100-101 0.7418846363327027 1102 1 0.17344082186200893 1102 2 0.2909494149292229 1102 3 0.2986224650109719 1102 4 0.20518597366861968 1102 5 0.14714204634668704 1102 6 0.2778542637661445 1102 7 0.25114226030247266 1102 8 0.16174761175941654 1102 9 0.3029982196567076 1102 10-11 0.33933522404835514 1102 12-13 0.34699737957788557 1102 14-15 0.45049663802131334 1102 16-17 0.39261410631038274 1102 18-19 0.3652032016037907 1102 20-21 0.34832479000633754 1102 22-23 0.34123317410052323 1102 24-25 0.4311722364628068 1102 26-27 0.42063722711401397 1102 28-29 0.4101487943509028 1102 30-31 0.33504282013031883 1102 32-33 0.215931339717919 1102 34-35 0.22051368821395556 1102 36-37 0.3724089345455468 1102 38-39 0.3992560260539335 1102 40-41 0.4167539522105912 1102 42-43 0.3337259684976672 1102 44-45 0.29298711514904596 1102 46-47 0.21311959553489856 1102 48-49 0.48975322484772477 1102 50-51 0.5012179953672486 1102 52-53 0.24538676408529625 1102 54-55 0.3081827962560837 1102 56-57 0.44377877208749794 1102 58-59 0.5742433467502934 1102 60-61 0.5838770728788845 1102 62-63 0.41605434264567265 1102 64-65 0.5704297676439012 1102 66-67 0.5670032377103702 1102 68-69 0.39825249196129775 1102 70-71 0.4899988329736473 1102 72-73 0.0807731617291374 1102 74-75 0.2570470114038237 1102 76-77 0.26969891556458236 1102 78-79 0.3202009001371593 1102 80-81 0.35922962136497105 1102 82-83 0.2117710576825722 1102 84-85 0.2558153144043658 1102 86-87 0.2942431147483191 1102 88-89 0.18899138497128476 1102 90-91 0.03126279901853124 1102 92-93 0.36077981054609864 1102 94-95 0.5125722296877413 1102 96-97 0.5481352521425968 1102 98-99 0.4565483870405238 1102 100-101 0.42363127484159335 1103 1 0.2878706706116958 1103 2 0.23239509495323318 1103 3 0.2603980852847272 1103 4 0.1591383915652429 1103 5 0.15563617985367273 1103 6 0.16652072063700984 1103 7 0.27853722998074915 1103 8 0.2567993908174273 1103 9 0.3430281934530015 1103 10-11 0.3778880588362341 1103 12-13 0.39687711049919017 1103 14-15 0.5919541839712608 1103 16-17 0.3973665784215612 1103 18-19 0.37962030162342586 1103 20-21 0.37007137121756273 1103 22-23 0.4372777183191445 1103 24-25 0.46714027272216896 1103 26-27 0.4884210044978232 1103 28-29 0.4567245010356018 1103 30-31 0.5657817219843153 1103 32-33 0.6160663308535277 1103 34-35 0.5460491348764833 1103 36-37 0.5551486080741981 1103 38-39 0.5984384455811451 1103 40-41 0.7957267005667532 1103 42-43 0.8189718450234906 1103 44-45 0.739574480041064 1103 46-47 0.6890765551990583 1103 48-49 0.8984847807328649 1103 50-51 0.6636870582758689 1103 52-53 0.8531781855141745 1103 54-55 0.8764020037887903 1103 56-57 0.7015282343559264 1103 58-59 0.8183229767203528 1103 60-61 0.7992220779212502 1103 62-63 0.6321520680229682 1103 64-65 0.6832090323407769 1103 66-67 0.8155244109881998 1103 68-69 0.5945939699415668 1103 70-71 0.557268247134445 1103 72-73 0.4552829457952505 1103 74-75 0.5848643768878468 1103 76-77 0.5574741037836155 1103 78-79 0.6112869932719533 1103 80-81 0.8050222029023182 1103 82-83 0.7799466443059089 1103 84-85 0.6364038539451613 1103 86-87 0.7856861463599749 1103 88-89 0.909519748429009 1103 90-91 0.668631274187323 1103 92-93 0.6547861677743398 1103 94-95 0.5886477447520129 1103 96-97 0.30957976894235983 1103 98-99 0.23992643180595863 1103 100-101 0.04344363222977776 1104 1 0.25173684195073065 1104 2 0.21968701963784554 1104 3 0.20895869280689539 1104 4 0.14589388113056145 1104 5 0.1470840360299306 1104 6 0.11765852182428205 1104 7 0.15572991792659252 1104 8 0.20774752297840848 1104 9 0.30361111126417484 1104 10-11 0.20883344994153674 1104 12-13 0.19755851589405893 1104 14-15 0.3746028755932329 1104 16-17 0.36407214770049023 1104 18-19 0.31949321585919677 1104 20-21 0.2241226840805055 1104 22-23 0.2794682026466475 1104 24-25 0.4193053434045666 1104 26-27 0.4960652675860473 1104 28-29 0.55267610712135 1104 30-31 0.4701636026541891 1104 32-33 0.5069818359331251 1104 34-35 0.45337126128425353 1104 36-37 0.3395571877288006 1104 38-39 0.4591589924493107 1104 40-41 0.5052519692666309 1104 42-43 0.6143752149184323 1104 44-45 0.42079884998035766 1104 46-47 0.26610836271982663 1104 48-49 0.33834579367570683 1104 50-51 0.38590975376587977 1104 52-53 0.5692694601626869 1104 54-55 0.5876969321091892 1104 56-57 0.560325281732851 1104 58-59 0.44155723199882857 1104 60-61 0.2658821717335087 1104 62-63 0.4007701374929411 1104 64-65 0.1922630348959231 1104 66-67 0.21548001290172536 1104 68-69 0.2006828467927484 1104 70-71 0.32978467906168163 1104 72-73 0.14272881057200593 1104 74-75 0.2094412232857472 1104 76-77 0.07445712351996647 1104 78-79 0.03823986390138856 1104 80-81 0.12316105115281317 1104 82-83 0.2603794154054597 1104 84-85 0.24425587602494048 1104 86-87 0.1874632724758456 1104 88-89 0.18728916050263322 1104 90-91 0.22150132735228212 1104 92-93 0.3411522720903015 1104 94-95 0.4662279137403509 1104 96-97 0.5189212566240187 1104 98-99 0.2967809125675842 1104 100-101 -0.04727503799241006 1105 1 0.2812901144868931 1105 2 0.30083951167757306 1105 3 0.24946497688029012 1105 4 0.17665477860529677 1105 5 0.10724082772412658 1105 6 0.17833740754925742 1105 7 0.14019259575255205 1105 8 0.2447460929658405 1105 9 0.33777201508597443 1105 10-11 0.2463644279374222 1105 12-13 0.38054687191814196 1105 14-15 0.4201461793934911 1105 16-17 0.23311985261205592 1105 18-19 0.32015064177887353 1105 20-21 0.29513562247263536 1105 22-23 0.30073699500248097 1105 24-25 0.37388561500117845 1105 26-27 0.31609609411792405 1105 28-29 0.3018839263185171 1105 30-31 0.4184957435089771 1105 32-33 0.5212850870719876 1105 34-35 0.4121283332109016 1105 36-37 0.4603225666148134 1105 38-39 0.4943136684557814 1105 40-41 0.3314130009913683 1105 42-43 0.3189042772552497 1105 44-45 0.3447651201479047 1105 46-47 0.06852476660845497 1105 48-49 0.14041575369882509 1105 50-51 0.24181074342689612 1105 52-53 0.2384953877234679 1105 54-55 0.12022611177049924 1105 56-57 0.25598203928753804 1105 58-59 0.36873682311332345 1105 60-61 0.3488108797887932 1105 62-63 0.5279397993002632 1105 64-65 0.28281122401147485 1105 66-67 0.32253128755550264 1105 68-69 0.374232831357844 1105 70-71 0.2790614264033664 1105 72-73 0.35151605509069483 1105 74-75 0.25010630414829293 1105 76-77 0.2867898279170369 1105 78-79 0.4072176068664888 1105 80-81 0.38436445914107153 1105 82-83 0.4205663075659736 1105 84-85 0.43310456684698906 1105 86-87 0.5073570601435051 1105 88-89 0.36938399909008623 1105 90-91 0.6129069229616526 1105 92-93 0.44292322292844943 1105 94-95 0.3076726255307918 1105 96-97 0.29925192419081625 1105 98-99 0.21810377517001456 1105 100-101 0.010728704851519666 1106 1 0.1201676299561818 1106 2 0.12858115365384037 1106 3 0.15811626207670315 1106 4 0.04069418534469804 1106 5 -0.01695403494542802 1106 6 0.01026418000311935 1106 7 0.03705871476746836 1106 8 0.05747050205190618 1106 9 0.08750855485983067 1106 10-11 0.056348058851241944 1106 12-13 0.09257512694764358 1106 14-15 0.20612073748574034 1106 16-17 -0.024561164718726047 1106 18-19 0.23946162850220531 1106 20-21 0.12692670571167497 1106 22-23 0.16486602140896878 1106 24-25 0.20567057962840352 1106 26-27 0.09186660730389917 1106 28-29 0.22024868772145823 1106 30-31 0.1565921641930501 1106 32-33 0.2675707297853691 1106 34-35 0.12552247834724994 1106 36-37 -0.2866418820910681 1106 38-39 -0.06164751507090216 1106 40-41 0.10778529764470335 1106 42-43 0.30892518131253865 1106 44-45 0.16916743974963566 1106 46-47 -0.07755025189134557 1106 48-49 0.1951629326588673 1106 50-51 0.06314727859380298 1106 52-53 -0.11204996054792105 1106 54-55 -0.2995408914917945 1106 56-57 0.060816678404613356 1106 58-59 -0.09721598151435984 1106 60-61 -0.39167215941926514 1106 62-63 -0.19831782736940085 1106 64-65 -0.6087666148084558 1106 66-67 -0.2325322596269146 1106 68-69 -0.9397307765787879 1106 70-71 -0.681969854819549 1106 72-73 -0.5199452277734515 1106 74-75 -0.26990335605999505 1106 76-77 -0.30392147150334736 1106 78-79 -0.3588833401506548 1106 80-81 -1.160581783455406 1106 82-83 -0.7676695454836846 1106 84-85 -0.5441917841568902 1106 86-87 -0.30582696466494497 1106 88-89 -0.2436289205387112 1106 90-91 -0.3477324071871237 1106 92-93 -0.5589494322035549 1106 94-95 -0.39808369247577957 1106 96-97 -0.4592381895721722 1106 98-99 -0.5992463044439198 1106 100-101 -0.6840727785879182 1107 1 0.16011720585597544 1107 2 0.21269302252323996 1107 3 0.25399817749411824 1107 4 0.1607719865186965 1107 5 0.08772276542890722 1107 6 0.17458808151549476 1107 7 0.1763130387039027 1107 8 0.24714333903609287 1107 9 0.29751445629538154 1107 10-11 0.26233120658616826 1107 12-13 0.3118054261050247 1107 14-15 0.3447594264591416 1107 16-17 0.3253422822085099 1107 18-19 0.32825378971964625 1107 20-21 0.36198996281522255 1107 22-23 0.25315394162434757 1107 24-25 0.3100247216330061 1107 26-27 0.3185690317812373 1107 28-29 0.2715782567071372 1107 30-31 0.3259560313444112 1107 32-33 0.40349859957665046 1107 34-35 0.32172146497649123 1107 36-37 0.5415303141159526 1107 38-39 0.41108505910735715 1107 40-41 0.49763692368225776 1107 42-43 0.5176289257559361 1107 44-45 0.5740401398484565 1107 46-47 0.6278794180123768 1107 48-49 0.673004613555328 1107 50-51 0.6666790607924185 1107 52-53 0.8916267385591823 1107 54-55 0.689796017622605 1107 56-57 0.7352456534055776 1107 58-59 0.6590679997546971 1107 60-61 0.5479896778477737 1107 62-63 0.7272720542876101 1107 64-65 0.6967042603849123 1107 66-67 0.7161493457032329 1107 68-69 0.6434906389975126 1107 70-71 0.5712900772266423 1107 72-73 0.7682127910203498 1107 74-75 0.6666164032731778 1107 76-77 0.49681533494702634 1107 78-79 0.6969826518666977 1107 80-81 0.6417438710277636 1107 82-83 0.5063445830418942 1107 84-85 0.6010320688170445 1107 86-87 0.5490734966247786 1107 88-89 0.5658793746448083 1107 90-91 0.8229189506668497 1107 92-93 0.31096581759478426 1107 94-95 0.1737119851591764 1107 96-97 0.208226043887219 1107 98-99 0.21272034022331 1107 100-101 -0.4398975653156256 1108 1 0.1459280258999769 1108 2 0.10085697666308846 1108 3 0.19236574195932832 1108 4 0.10261732466215534 1108 5 0.10964367169983547 1108 6 -0.002859000297597447 1108 7 0.11478104181954762 1108 8 0.17193118800916096 1108 9 0.19073655553150104 1108 10-11 0.17864394657988214 1108 12-13 0.18728131399636538 1108 14-15 0.27866430354136895 1108 16-17 0.30597982849911176 1108 18-19 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-0.3093244835572264 2316 62-63 -0.4656327994173708 2316 64-65 -0.19618998155161904 2316 66-67 -0.4048336577809657 2316 68-69 -0.10306259965526365 2316 70-71 -0.25697142032970177 2316 72-73 -0.22440318676568083 2316 74-75 0.06767993828906427 2316 76-77 0.016690587713451777 2316 78-79 0.04912003889557681 2316 80-81 -0.12469464261720375 2316 82-83 -0.16464755835401945 2316 84-85 -0.08885904264779043 2316 86-87 0.07751244653133682 2316 88-89 0.5039078259751122 2316 90-91 0.32717485935847535 2316 92-93 0.31575879967858356 2316 94-95 0.28208467046718155 2316 96-97 0.4039736724517411 2316 98-99 0.48609364455114346 2316 100-101 0.5300811521932047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 24.0 5 68.0 6 158.0 7 365.0 8 832.0 9 1494.0 10 2506.0 11 3858.0 12 5421.0 13 7539.0 14 9802.0 15 12927.0 16 16070.0 17 19702.0 18 19346.0 19 17195.0 20 17291.0 21 18205.0 22 19594.0 23 21863.0 24 25262.0 25 29408.0 26 35912.0 27 42781.0 28 51200.0 29 60839.0 30 72199.0 31 85971.0 32 103653.0 33 126881.0 34 161951.0 35 212184.0 36 280127.0 37 319459.0 38 165093.0 39 8287.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.27858917481832 19.193426570311438 15.370383119341845 23.15760113552839 2 16.109547332522354 20.18908497175576 39.26835419703205 24.43301349868984 3 19.52162173380863 23.65689918226889 29.705396093883675 27.116082990038805 4 13.608128855706534 16.851338990352225 36.03127542102405 33.509256732917194 5 14.04990039376382 37.02808632405364 34.185154111011805 14.736859171170732 6 33.16058215695317 35.971644374205574 16.66566269176975 14.202110777071509 7 29.37841467470454 32.128934674643794 20.040739445228784 18.45191120542288 8 26.729743287837245 35.22124485125395 19.069330456734335 18.979681404174478 9 26.09010160057221 16.82329518734931 19.75240460424575 37.334198607832725 10-11 25.260126521085247 26.43394191085614 27.0124845543946 21.293447013664014 12-13 26.23674942963258 23.438114565515946 27.383457497186757 22.941678507664715 14-15 22.707811414030303 25.11545289476335 25.51373564210438 26.663000049101964 16-17 22.534689020041196 28.258333033828215 26.47851333422088 22.728464611909708 18-19 22.578399893494293 26.692005643182597 28.631837005772265 22.097757457550845 20-21 22.938944996180677 25.9090570853377 28.958618179572472 22.193379738909158 22-23 22.74167658408256 25.77190061559691 28.932902923389292 22.55351987693124 24-25 22.685164444802165 26.051003793760557 28.886187436423693 22.377644325013584 26-27 22.211920000081 26.423301348350787 28.685942706482347 22.678835945085865 28-29 22.395988622517756 26.765177695963665 28.503589758646118 22.335243922872454 30-31 22.4706033619154 26.293343241468786 28.48012711840812 22.755926278207685 32-33 22.165487797655356 26.2520621560015 28.48789737790442 23.094552668438727 34-35 22.935694819853456 26.4092498530568 28.2748336542205 22.380221672869247 36-37 22.98742380325167 25.96538660516594 28.32775156190046 22.71943802968193 38-39 22.460746329139813 26.354866397166937 28.30311607058561 22.88127120310764 40-41 22.95776298500859 26.13132918000703 28.429968241304326 22.480939593680052 42-43 22.67416461779027 26.1806666049093 28.18557884618013 22.959589931120295 44-45 22.59738833159831 26.406967823777556 28.381145751628527 22.614498092995603 46-47 23.045551944058182 26.71048215599138 27.611857567853093 22.63210833209735 48-49 22.74932029212126 26.75292751486853 27.5658687681633 22.93188342484691 50-51 22.947626426171652 26.895778800201064 27.589812303940153 22.56678246968713 52-53 23.266131134645182 26.562619432938106 27.543216057253904 22.628033375162808 54-55 22.742942098658503 26.77689636093691 27.744863149784788 22.735298390619803 56-57 22.801940388189003 26.875960841941787 27.548176874391604 22.77392189547761 58-59 22.741094447377627 26.574439339077422 27.496417328235502 23.18804888530945 60-61 22.37829672874606 26.79322149896658 27.835195580215654 22.9932861920717 62-63 22.580551268273396 26.840678295564473 27.843699838165996 22.735070597996135 64-65 22.326284079674775 26.863913143178802 27.629422910167197 23.18037986697923 66-67 22.217213592953 26.879575313234078 27.633569470095743 23.26964162371718 68-69 22.78082066961619 26.514077733793652 27.751865388314624 22.95323620827554 70-71 22.59649431859311 26.618278402352036 27.344001125802908 23.441226153251947 72-73 22.570306312818577 26.781762715450746 27.386602904964136 23.26132806676654 74-75 22.473919010104375 27.059106111372373 27.249869778550135 23.21710509997312 76-77 22.585107120746795 26.72642763964827 27.47419489228193 23.21427034732301 78-79 22.670706526663633 26.928909971799275 27.37178945279656 23.028594048740533 80-81 22.68713351788158 26.963440565196517 27.18184600050267 23.167579916419236 82-83 22.650175667812306 27.065523676366254 27.22166440601348 23.062636249807955 84-85 22.560465208696186 27.14337530940383 27.22654953764393 23.069609944256054 86-87 22.779087617521732 27.120668511832218 26.94197346656998 23.15827040407607 88-89 22.62128457011417 27.291192308899415 26.68683225021995 23.400690870766468 90-91 22.411556111278447 27.24000901081044 26.897826015424688 23.450608862486426 92-93 22.519926943819836 26.920584813912264 27.10064359497962 23.458844647288284 94-95 22.273261895788792 26.771691010557696 27.24180496016535 23.713242133488166 96-97 22.512542514962178 26.467781770616877 27.205070562561723 23.81460515185922 98-99 22.221611962662237 26.7703798570474 26.85992771377083 24.148080466519527 100-101 22.31581691538259 26.48417093567133 27.26785388867516 23.93215826027092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 281.0 1 203.5 2 175.0 3 242.5 4 224.0 5 228.0 6 403.0 7 940.0 8 1511.5 9 1667.0 10 1403.5 11 1152.5 12 1169.5 13 1242.5 14 1405.5 15 1571.0 16 1679.5 17 1847.5 18 2054.0 19 2321.5 20 2709.0 21 3243.0 22 3979.5 23 4907.0 24 6070.0 25 7511.5 26 9661.0 27 12417.0 28 14952.5 29 17738.5 30 21102.0 31 25282.5 32 31890.0 33 35136.5 34 35207.0 35 37734.0 36 41558.5 37 45862.5 38 50037.0 39 53211.0 40 56139.0 41 60103.5 42 63005.0 43 65003.5 44 67334.5 45 70816.5 46 73065.5 47 74977.0 48 75704.5 49 74492.5 50 73366.5 51 71228.0 52 69443.0 53 69347.5 54 70572.5 55 67218.5 56 61344.5 57 56303.0 58 52494.0 59 47031.5 60 37954.0 61 31511.5 62 26824.5 63 20036.5 64 13981.0 65 10597.5 66 8008.0 67 6139.0 68 4781.5 69 3797.5 70 2967.0 71 2088.5 72 1525.5 73 1287.5 74 1021.0 75 744.0 76 523.5 77 349.5 78 240.0 79 141.0 80 68.5 81 42.0 82 30.5 83 17.0 84 9.0 85 4.5 86 2.0 87 1.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.58067579490767E-4 2 0.046520315811693455 3 7.593087455662696E-4 4 0.0 5 0.08651057641151699 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.531029151887566E-5 26-27 0.009061084363757485 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011541492932607299 36-37 1.5186174911325395E-4 38-39 0.004682403930991996 40-41 0.005897297923898028 42-43 4.0496466430201054E-4 44-45 2.531029151887566E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.593087455662697E-5 68-69 0.0010630322437927776 70-71 1.0124116607550264E-4 72-73 2.531029151887566E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.00134144545050041 82-83 9.871013692361505E-4 84-85 0.005542953842633769 86-87 0.0024550982773309383 88-89 1.5186174911325395E-4 90-91 0.0038471643108691 92-93 7.593087455662696E-4 94-95 1.2655145759437827E-4 96-97 0.0 98-99 5.062058303775132E-5 100-101 5.062058303775132E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.880055644553565 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62717067887974 45.5094501406737 2 12.422243025269564 14.380002350655396 3 3.8621369416616558 6.7062210327078775 4 1.7299933344227345 4.005284418443785 5 0.8991543219163048 2.602155109277827 6 0.5185572993932986 1.800847520626413 7 0.35419116348902663 1.4350422976087822 8 0.24717495200173037 1.1445199980640008 9 0.1908882306722836 0.9943759271881936 >10 1.037938960975882 10.988004205450878 >50 0.06945801010013956 2.754511558173817 >100 0.03567397605829126 3.807223145633477 >500 0.002884362423591073 1.1954927023001582 >1k 0.002447337813956057 2.393110185333105 >5k 8.740492192700204E-5 0.2837594078626202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5609 0.28393085025874715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4544 0.23001992932354196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3333 0.16871840326482512 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2435 0.12326111969692445 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2347 0.11880650838960233 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2297 0.11627547923771477 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2270 0.11490872349569548 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2148 0.10873301236508982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.062058303775131E-5 0.0 2 0.0 0.0 0.0 5.062058303775131E-5 0.0 3 0.0 0.0 0.0 5.062058303775131E-5 0.0 4 0.0 0.0 0.0 5.062058303775131E-5 0.0 5 0.0 5.062058303775131E-5 0.0 5.062058303775131E-5 0.0 6 0.0 5.062058303775131E-5 0.0 5.062058303775131E-5 0.0 7 0.0 5.062058303775131E-5 0.0 5.062058303775131E-5 0.0 8 0.0 5.062058303775131E-5 0.0 1.0124116607550262E-4 0.0 9 0.0 5.062058303775131E-5 0.0 1.0124116607550262E-4 0.0 10-11 0.0 5.062058303775131E-5 0.0 1.0124116607550262E-4 0.0 12-13 5.062058303775131E-5 5.062058303775131E-5 0.0 2.5310291518875654E-4 5.062058303775131E-5 14-15 1.0124116607550262E-4 1.0124116607550262E-4 0.0 2.5310291518875654E-4 1.0124116607550262E-4 16-17 1.0124116607550262E-4 1.0124116607550262E-4 0.0 2.5310291518875654E-4 1.5186174911325395E-4 18-19 1.5186174911325395E-4 1.0124116607550262E-4 0.0 2.5310291518875654E-4 2.0248233215100524E-4 20-21 2.0248233215100524E-4 1.0124116607550262E-4 0.0 2.784132067076322E-4 2.784132067076322E-4 22-23 2.0248233215100524E-4 1.0124116607550262E-4 0.0 3.7965437278313487E-4 3.037234982265079E-4 24-25 2.5310291518875654E-4 1.5186174911325395E-4 0.0 5.315161218963887E-4 3.037234982265079E-4 26-27 2.5310291518875654E-4 1.5186174911325395E-4 0.0 7.846190370851454E-4 3.037234982265079E-4 28-29 4.049646643020105E-4 1.5186174911325395E-4 0.0 0.0026069600264441925 3.037234982265079E-4 30-31 4.049646643020105E-4 1.5186174911325395E-4 0.0 0.009668531360210501 3.290337897453835E-4 32-33 4.049646643020105E-4 2.0248233215100524E-4 0.0 0.023994156359894124 3.543440812642592E-4 34-35 4.302749558208861E-4 2.0248233215100524E-4 0.0 0.046140661438910324 3.543440812642592E-4 36-37 4.555852473397618E-4 2.0248233215100524E-4 0.0 0.08203065481267599 3.543440812642592E-4 38-39 5.062058303775131E-4 2.0248233215100524E-4 0.0 0.13604281691395664 3.543440812642592E-4 40-41 5.062058303775131E-4 2.0248233215100524E-4 0.0 0.1954713814002767 3.7965437278313487E-4 42-43 6.074469964530158E-4 2.0248233215100524E-4 0.0 0.2567222868759558 4.049646643020105E-4 44-45 6.074469964530158E-4 2.0248233215100524E-4 0.0 0.3306536484025916 4.049646643020105E-4 46-47 7.593087455662697E-4 2.0248233215100524E-4 0.0 0.42278310953129894 4.049646643020105E-4 48-49 8.09929328604021E-4 2.277926236698809E-4 0.0 0.521695728787065 4.049646643020105E-4 50-51 8.09929328604021E-4 2.784132067076322E-4 0.0 0.6299984661963339 4.049646643020105E-4 52-53 8.352396201228966E-4 3.037234982265079E-4 0.0 0.7373394125278856 4.049646643020105E-4 54-55 8.605499116417723E-4 3.037234982265079E-4 0.0 0.8592843970658286 4.049646643020105E-4 56-57 8.605499116417723E-4 3.037234982265079E-4 0.0 1.0008195472393813 4.302749558208861E-4 58-59 8.605499116417723E-4 3.037234982265079E-4 0.0 1.1525547448950408 4.555852473397618E-4 60-61 8.605499116417723E-4 3.290337897453835E-4 0.0 1.316767916269506 5.062058303775131E-4 62-63 8.605499116417723E-4 3.543440812642592E-4 0.0 1.4821706713453584 5.062058303775131E-4 64-65 8.605499116417723E-4 3.543440812642592E-4 0.0 1.6539263095924488 5.062058303775131E-4 66-67 8.605499116417723E-4 3.543440812642592E-4 0.0 1.8575222945702845 5.568264134152644E-4 68-69 8.605499116417723E-4 3.543440812642592E-4 0.0 2.083340715501693 5.568264134152644E-4 70-71 9.364807861983993E-4 3.543440812642592E-4 0.0 2.3231810379345585 5.568264134152644E-4 72-73 9.617910777172749E-4 3.543440812642592E-4 0.0 2.574942507672815 6.327572879718914E-4 74-75 9.617910777172749E-4 3.543440812642592E-4 0.0 2.834651408947998 7.086881625285184E-4 76-77 9.617910777172749E-4 4.049646643020105E-4 0.0 3.119746532616613 7.086881625285184E-4 78-79 9.617910777172749E-4 4.049646643020105E-4 0.0 3.442452749482278 7.086881625285184E-4 80-81 9.617910777172749E-4 4.049646643020105E-4 0.0 3.7759158402434645 7.086881625285184E-4 82-83 0.0010124116607550262 4.049646643020105E-4 0.0 4.130386472965318 7.086881625285184E-4 84-85 0.0010124116607550262 4.049646643020105E-4 0.0 4.484933036561729 7.086881625285184E-4 86-87 0.0010124116607550262 4.049646643020105E-4 0.0 4.85226129737517 7.339984540473941E-4 88-89 0.0010124116607550262 4.049646643020105E-4 0.0 5.253176315034161 7.593087455662697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3725 0.0 48.650276 1 GTATCAA 6145 0.0 37.928078 1 ATCAACG 7790 0.0 29.660353 3 TCAACGC 7960 0.0 29.08663 4 TATCAAC 8035 0.0 28.888205 2 CAACGCA 8175 0.0 28.263508 5 AACGCAG 8545 0.0 27.06858 6 ACGCAGA 9640 0.0 23.993881 7 CGCAGAG 9850 0.0 23.482338 8 TAAGGTG 1250 0.0 22.820162 5 GGACCGA 615 0.0 22.396051 6 TACATGG 6390 0.0 21.735449 2 GAGTACT 6395 0.0 21.6123 12-13 TAGCGAT 110 0.0028798222 21.58867 7 GTACATG 6700 0.0 21.439703 1 TGGTATC 1975 0.0 21.43431 2 ACATGGG 6410 0.0 20.841347 3 GCAGAGT 11050 0.0 20.674328 9 GTAAGGT 1270 0.0 20.58906 4 AGAGTAC 9365 0.0 20.235487 10-11 >>END_MODULE