FastQCFastQC Report
Thu 26 May 2016
SRR938349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972871
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55580.2817214100668518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.22008534769886118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.15601628286897623No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24410.12372831269758641No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22430.11369217754227214No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22200.11252636386261444No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21200.10745760873366783No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20910.10598766974627331No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19940.10107097727119513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.054.5387461
GTATCAA54350.042.2542881
ATCAACG71400.031.8163153
TCAACGC71750.031.6611124
TATCAAC73200.031.1637942
CAACGCA72800.031.0086155
AACGCAG76750.029.5924916
ACGCAGA86350.025.8583877
TAAGGTG12950.025.3220885
CGCAGAG88900.025.063238
TACATGG61900.024.8756542
GTACATG65850.023.8770941
TATACCG1000.001649049323.7623925
GTAAGGT12050.023.2693964
ACATGGG65100.022.5578463
ATACCGT2554.8930815E-1022.3600656
GCAGAGT98550.022.2704649
AGGTAAG11450.021.9983712
TGGTATC13250.021.161982
AGAGTAC86800.021.12357710-11
Produced by FastQC (version 0.11.5)