##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938349_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1972871 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.941735673543786 31.0 31.0 34.0 27.0 34.0 2 31.08909959140765 31.0 31.0 34.0 27.0 34.0 3 31.143766115473337 31.0 31.0 34.0 27.0 34.0 4 34.81055375642908 37.0 35.0 37.0 32.0 37.0 5 34.81099017624568 37.0 35.0 37.0 32.0 37.0 6 34.81123651774495 37.0 35.0 37.0 32.0 37.0 7 34.71476087387366 37.0 35.0 37.0 31.0 37.0 8 34.66241431903049 37.0 35.0 37.0 30.0 37.0 9 36.12379927526939 39.0 35.0 39.0 30.0 39.0 10-11 35.97312698093286 38.5 35.0 39.0 30.0 39.0 12-13 35.78698936727237 38.0 35.0 39.0 30.0 39.0 14-15 36.73416609600932 39.0 36.0 41.0 30.0 41.0 16-17 36.539748163970174 39.0 36.0 40.5 30.0 41.0 18-19 36.3975488007072 39.0 36.0 40.0 29.0 41.0 20-21 36.36391152792048 39.0 35.5 40.0 29.5 41.0 22-23 36.46042645464402 39.0 36.0 41.0 29.5 41.0 24-25 36.35784017302703 39.0 35.5 41.0 29.0 41.0 26-27 36.14742854449176 39.0 35.0 40.5 28.0 41.0 28-29 35.94053944733335 39.0 35.0 40.0 27.0 41.0 30-31 35.65392846263136 38.0 34.5 40.0 27.0 41.0 32-33 35.32724389987992 38.0 34.0 40.0 26.5 41.0 34-35 35.04281400050992 38.0 34.0 40.0 25.0 41.0 36-37 34.71949483772634 38.0 33.5 40.0 24.5 41.0 38-39 34.36801975395249 38.0 33.0 40.0 24.0 41.0 40-41 34.223019650043014 38.0 33.0 40.0 24.0 41.0 42-43 33.959483159314516 38.0 33.0 40.0 22.5 41.0 44-45 33.63278339029769 38.0 32.5 40.0 21.0 41.0 46-47 33.21289607886172 37.5 31.5 40.0 18.5 41.0 48-49 32.78785764502595 37.0 31.0 40.0 15.0 41.0 50-51 31.989075312070582 36.0 30.5 39.0 13.5 40.5 52-53 31.982535604203214 36.5 30.5 39.0 11.0 40.0 54-55 32.008795303899745 36.5 30.5 39.0 8.5 41.0 56-57 31.628947356416106 36.0 30.0 39.0 7.5 41.0 58-59 31.135513168372388 35.5 29.0 39.0 4.0 40.5 60-61 30.598286710078867 35.0 28.5 39.0 2.0 40.0 62-63 29.96481929127652 35.0 28.0 38.0 2.0 40.0 64-65 29.415647044332854 34.0 26.5 38.0 2.0 40.0 66-67 28.807499831463893 34.0 26.0 37.5 2.0 40.0 68-69 28.133735302510907 34.0 25.5 37.0 2.0 39.0 70-71 27.45319435482604 33.0 23.5 36.0 2.0 39.0 72-73 26.765993316339486 32.5 22.0 36.0 2.0 39.0 74-75 26.024473216951336 32.0 20.0 35.0 2.0 38.0 76-77 25.247501990753577 31.5 17.0 35.0 2.0 37.0 78-79 24.405681111436074 31.0 9.0 35.0 2.0 36.5 80-81 23.59771774231564 30.5 4.5 34.5 2.0 36.0 82-83 22.829630776670143 30.0 2.0 34.0 2.0 36.0 84-85 22.053701433089138 29.0 2.0 34.0 2.0 35.0 86-87 21.30269186378633 29.0 2.0 34.0 2.0 35.0 88-89 20.492871556224404 27.5 2.0 34.0 2.0 35.0 90-91 19.64869167826989 26.0 2.0 33.5 2.0 35.0 92-93 18.774298471618266 25.0 2.0 33.0 2.0 35.0 94-95 17.873841726093595 23.5 2.0 33.0 2.0 35.0 96-97 16.817664459561726 17.0 2.0 32.0 2.0 34.5 98-99 15.591545772632879 2.0 2.0 32.0 2.0 34.0 100-101 12.977955983944213 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6786030883833725 1101 2 0.5810089598921948 1101 3 0.6565174195662813 1101 4 0.6083625543069076 1101 5 0.6135836574000351 1101 6 0.5643944254559159 1101 7 0.5380226906262351 1101 8 0.4484878504452823 1101 9 0.6583238880289386 1101 10-11 0.7453265175605921 1101 12-13 0.8542750642032786 1101 14-15 1.0319488026724741 1101 16-17 1.0788651090471646 1101 18-19 1.1252018597969666 1101 20-21 1.107172690832229 1101 22-23 0.9828816554699173 1101 24-25 1.0644063722223294 1101 26-27 1.1147782870857768 1101 28-29 1.2521054832504959 1101 30-31 0.995456958821606 1101 32-33 1.1368910789987865 1101 34-35 1.2155181871696357 1101 36-37 1.3890326954270265 1101 38-39 1.6353027671216012 1101 40-41 1.653136583616451 1101 42-43 1.69833887666055 1101 44-45 1.7422862133278514 1101 46-47 1.8882143563751868 1101 48-49 1.9484116409787617 1101 50-51 1.9773467843383727 1101 52-53 2.1545803197573044 1101 54-55 2.460748549823684 1101 56-57 2.6014146249025565 1101 58-59 2.726139897018168 1101 60-61 2.6750832729128575 1101 62-63 2.750952339603593 1101 64-65 2.8808567142767494 1101 66-67 3.0502957408109275 1101 68-69 3.3198862813650756 1101 70-71 3.293911545525358 1101 72-73 3.6260766782580767 1101 74-75 3.8939027441566516 1101 76-77 3.921551045201973 1101 78-79 4.214133905022557 1101 80-81 4.723966780457989 1101 82-83 4.956231791662123 1101 84-85 5.251195664749805 1101 86-87 5.358709556991137 1101 88-89 5.725066119698859 1101 90-91 5.96324002500635 1101 92-93 6.1744186439359225 1101 94-95 6.384305994787933 1101 96-97 6.340757474995545 1101 98-99 6.56347294793215 1101 100-101 6.276383681077441 1102 1 0.5610658591414968 1102 2 0.5430934213843734 1102 3 0.4361160099049428 1102 4 0.594475724755597 1102 5 0.5269125855416874 1102 6 0.5673988199032252 1102 7 0.6505551923453368 1102 8 0.5695572913082714 1102 9 0.6324954971057792 1102 10-11 0.5380962281072641 1102 12-13 0.6278525096218814 1102 14-15 0.8274632968275455 1102 16-17 1.0032168206252905 1102 18-19 1.08422954803833 1102 20-21 1.1268902213462368 1102 22-23 0.9154866548252514 1102 24-25 0.8543226467194955 1102 26-27 0.9157921526951966 1102 28-29 0.9727315845484128 1102 30-31 1.006801133224215 1102 32-33 0.7421660274258102 1102 34-35 0.8942784480429395 1102 36-37 0.8928270696749152 1102 38-39 1.1311436215138997 1102 40-41 1.2679341328256655 1102 42-43 1.4655028733512836 1102 44-45 1.5154510749403656 1102 46-47 1.5737931287234659 1102 48-49 1.5319634879359612 1102 50-51 1.6910552573485162 1102 52-53 1.7397682656486602 1102 54-55 1.9713693595194073 1102 56-57 2.0145817159718327 1102 58-59 1.9347748785378478 1102 60-61 2.2136520691136496 1102 62-63 2.3294030283177065 1102 64-65 2.539253573697419 1102 66-67 2.7070080173280324 1102 68-69 2.8174708126956567 1102 70-71 2.9469033153379733 1102 72-73 3.1083319483715357 1102 74-75 3.2302015235962287 1102 76-77 3.3394079194498687 1102 78-79 3.7211096872126888 1102 80-81 4.150285517564747 1102 82-83 4.379810848736216 1102 84-85 4.747482544799659 1102 86-87 4.917072303037209 1102 88-89 5.119176861919936 1102 90-91 5.439996998102444 1102 92-93 5.3940503537949525 1102 94-95 5.675817271004195 1102 96-97 5.695133656876237 1102 98-99 6.042147656759726 1102 100-101 5.892411460645953 1103 1 0.6448579884845387 1103 2 0.6636823602462663 1103 3 0.5082751332739193 1103 4 0.5007349936088801 1103 5 0.5290284601314497 1103 6 0.5540133240396301 1103 7 0.6536532229985212 1103 8 0.6560047636976876 1103 9 0.6632587641037944 1103 10-11 0.5963843861746554 1103 12-13 0.7290348011784928 1103 14-15 0.9935106704013634 1103 16-17 1.030536280645542 1103 18-19 1.2780415474550395 1103 20-21 1.2322090464215094 1103 22-23 1.0030957582771975 1103 24-25 1.1068661439724607 1103 26-27 1.0679890388561404 1103 28-29 1.1245799553041067 1103 30-31 1.254935493217154 1103 32-33 1.2688508033285828 1103 34-35 1.3649104165576063 1103 36-37 1.6336315194027407 1103 38-39 1.6195244413755248 1103 40-41 1.6623815728678508 1103 42-43 1.9343494830085532 1103 44-45 1.9865957732671546 1103 46-47 2.259742792768705 1103 48-49 2.1479670753490225 1103 50-51 2.2583999899529417 1103 52-53 2.3255657933283693 1103 54-55 2.5863334006076997 1103 56-57 2.4632724745484857 1103 58-59 2.71280134744811 1103 60-61 2.9572555984060287 1103 62-63 2.9843097782732926 1103 64-65 3.108926785723387 1103 66-67 3.0376985291265584 1103 68-69 3.134225795148584 1103 70-71 3.3861994185653153 1103 72-73 3.6034559935084545 1103 74-75 3.767574974809161 1103 76-77 3.790126380305665 1103 78-79 4.032838065366512 1103 80-81 4.41840081877362 1103 82-83 4.63425878963886 1103 84-85 4.699046338118546 1103 86-87 4.765384364148449 1103 88-89 5.23874451499476 1103 90-91 5.550136996427696 1103 92-93 5.49894957780036 1103 94-95 5.737225111505179 1103 96-97 5.870553251424475 1103 98-99 5.937246737132961 1103 100-101 5.723998043115884 1104 1 0.6336182463941533 1104 2 0.5904546462387543 1104 3 0.6051659578645001 1104 4 0.41263512290073834 1104 5 0.33612213935332846 1104 6 0.4900729633458383 1104 7 0.5455851089669181 1104 8 0.4792052785850842 1104 9 0.6342946131636751 1104 10-11 0.704166011068672 1104 12-13 0.7701822992187957 1104 14-15 0.8453610903582529 1104 16-17 0.949322681928102 1104 18-19 1.1279770006592926 1104 20-21 1.116072025308796 1104 22-23 0.9512370113003925 1104 24-25 1.0215317856226207 1104 26-27 1.204300248338427 1104 28-29 1.0065879418083838 1104 30-31 1.1801976394520182 1104 32-33 1.3163052754711089 1104 34-35 1.4705802992878816 1104 36-37 1.6470133625836425 1104 38-39 1.60162952687989 1104 40-41 1.6951228699548508 1104 42-43 1.6947995832177938 1104 44-45 1.68234827440903 1104 46-47 1.8463696979152786 1104 48-49 2.0493773899988525 1104 50-51 2.0214961494371835 1104 52-53 2.2392062945654096 1104 54-55 2.4393282901470528 1104 56-57 2.4496146749188874 1104 58-59 2.7994845095447083 1104 60-61 2.8837006275592785 1104 62-63 3.050689881453991 1104 64-65 3.2472512257498884 1104 66-67 3.393295240647607 1104 68-69 3.656338617842291 1104 70-71 3.7302759706437634 1104 72-73 3.8479428743629285 1104 74-75 3.901537890326832 1104 76-77 4.122501610692744 1104 78-79 4.305630618235032 1104 80-81 4.740598869507473 1104 82-83 5.1212616993870945 1104 84-85 5.338416364366008 1104 86-87 5.484422162127508 1104 88-89 5.828598140358668 1104 90-91 6.0153363727933815 1104 92-93 5.920628503277747 1104 94-95 6.078820657739101 1104 96-97 6.167838766845943 1104 98-99 6.490796538288182 1104 100-101 6.043662920216754 1105 1 0.5091694467861174 1105 2 0.5590974683847634 1105 3 0.5331424399811091 1105 4 0.5008271856150941 1105 5 0.35180072828032394 1105 6 0.5410283773585789 1105 7 0.5181475783446743 1105 8 0.4101214961255053 1105 9 0.5269942701945425 1105 10-11 0.5767774760368312 1105 12-13 0.683950060120317 1105 14-15 0.98622341274924 1105 16-17 0.8944476206836143 1105 18-19 1.0870656826779097 1105 20-21 1.0903493403504356 1105 22-23 1.0683364723488964 1105 24-25 0.9536728929229668 1105 26-27 1.1692684012046755 1105 28-29 1.1729279712057448 1105 30-31 1.1177282616960156 1105 32-33 1.2352175733642952 1105 34-35 1.2643088637170834 1105 36-37 1.4370672577330836 1105 38-39 1.4276951809710212 1105 40-41 1.563324731787283 1105 42-43 1.777602130988491 1105 44-45 1.7286491073340642 1105 46-47 1.737109531330269 1105 48-49 1.7358056114589218 1105 50-51 1.826003743753688 1105 52-53 1.9535620025516813 1105 54-55 2.027765330813395 1105 56-57 2.1865083544877315 1105 58-59 2.399190536002326 1105 60-61 2.6085903874808807 1105 62-63 2.9139921842467373 1105 64-65 3.348182141967431 1105 66-67 3.4315118011571677 1105 68-69 3.4539868295032434 1105 70-71 3.2256213895560606 1105 72-73 3.661269674950873 1105 74-75 3.6805677776369734 1105 76-77 3.7839420810504123 1105 78-79 4.186571333570651 1105 80-81 4.557845317327171 1105 82-83 4.731501777780043 1105 84-85 4.729646153684971 1105 86-87 4.934201681970727 1105 88-89 5.373429105571798 1105 90-91 5.42224475103934 1105 92-93 5.478198321994064 1105 94-95 5.7111087518106345 1105 96-97 5.75064131461664 1105 98-99 5.952579977778628 1105 100-101 5.321222939814987 1106 1 0.18491796991732556 1106 2 0.2636301229763802 1106 3 0.24144251319923882 1106 4 0.33626543046714374 1106 5 0.2120972341790619 1106 6 0.12523676587279198 1106 7 0.25952697535185365 1106 8 0.08013655383235374 1106 9 0.309126892425617 1106 10-11 0.10103113101931172 1106 12-13 -0.08511591446468714 1106 14-15 0.4631145838970383 1106 16-17 0.3008557508400216 1106 18-19 0.4693754980480662 1106 20-21 0.40731670239386375 1106 22-23 0.31083987176204886 1106 24-25 0.49504305089843825 1106 26-27 0.5281577499175683 1106 28-29 0.3513865704105612 1106 30-31 0.5617808983265746 1106 32-33 0.8525924102152018 1106 34-35 0.7183014777574925 1106 36-37 0.7014507665880672 1106 38-39 0.663104631638781 1106 40-41 0.7374895747823942 1106 42-43 0.5385912531057571 1106 44-45 0.8415371496574409 1106 46-47 1.3170017841149644 1106 48-49 1.2883286689465194 1106 50-51 0.7220399837358684 1106 52-53 1.1887712343070582 1106 54-55 1.0682076362916817 1106 56-57 1.2131862978792647 1106 58-59 0.5744542493223506 1106 60-61 1.723759944057619 1106 62-63 2.156686467799325 1106 64-65 2.2915142059312075 1106 66-67 2.237549699856686 1106 68-69 1.678194552615686 1106 70-71 2.2143260749179596 1106 72-73 2.677122034266869 1106 74-75 3.163117855702005 1106 76-77 3.2845728962848604 1106 78-79 3.72144389118116 1106 80-81 4.084462704371035 1106 82-83 4.416433204298503 1106 84-85 4.535787572623118 1106 86-87 4.577746435146164 1106 88-89 4.519295225300549 1106 90-91 4.969432267719263 1106 92-93 5.093220676381776 1106 94-95 5.145831881247791 1106 96-97 5.213014639952387 1106 98-99 5.092593434212885 1106 100-101 4.177180451758115 1107 1 0.3801432987970692 1107 2 0.4100292582754541 1107 3 0.31513605960432045 1107 4 0.39421832582379324 1107 5 0.327999075849867 1107 6 0.39553966440028177 1107 7 0.4385331221526272 1107 8 0.2018706666602128 1107 9 0.4038035599262244 1107 10-11 0.44237649081308206 1107 12-13 0.6308323632998096 1107 14-15 0.7560891676273087 1107 16-17 0.8529894997984684 1107 18-19 0.8250168562306328 1107 20-21 0.9136649043367484 1107 22-23 0.8605441465540835 1107 24-25 0.8919715321129686 1107 26-27 0.9996759778846354 1107 28-29 1.0694166904544815 1107 30-31 1.0236956357117606 1107 32-33 1.178434409954562 1107 34-35 1.193300670526753 1107 36-37 1.354405080115562 1107 38-39 1.3974015545681056 1107 40-41 1.5011086027557852 1107 42-43 1.6107114545255072 1107 44-45 1.676903426832375 1107 46-47 1.4734738814583963 1107 48-49 1.5183314727666897 1107 50-51 1.7372054683611964 1107 52-53 1.6819931674748467 1107 54-55 2.05167520222502 1107 56-57 2.1560601461816766 1107 58-59 2.317198057978697 1107 60-61 2.302886559883852 1107 62-63 2.627362155937398 1107 64-65 2.775670299036623 1107 66-67 2.9379703425703205 1107 68-69 3.264661973828222 1107 70-71 3.333282384802576 1107 72-73 3.3299086296370604 1107 74-75 3.4154196248033486 1107 76-77 3.601746747769731 1107 78-79 3.8578200082084777 1107 80-81 3.9539555613662145 1107 82-83 4.2236072671733 1107 84-85 4.4153833579262205 1107 86-87 4.837343131052002 1107 88-89 5.03799185079729 1107 90-91 5.204362082780957 1107 92-93 5.283234546812764 1107 94-95 5.240863962572053 1107 96-97 5.117074046559974 1107 98-99 5.239762400499908 1107 100-101 4.725565281766933 1108 1 0.4160271074011881 1108 2 0.38098965119375094 1108 3 0.31279650799510605 1108 4 0.376538559362281 1108 5 0.39761762064831174 1108 6 0.42559622893850246 1108 7 0.39663662699279456 1108 8 0.5329548802157831 1108 9 0.4110351302051498 1108 10-11 0.6272750159958349 1108 12-13 0.6191121690018733 1108 14-15 0.5570839635615812 1108 16-17 0.9738913638617959 1108 18-19 0.9840464974955694 1108 20-21 0.8671876362663511 1108 22-23 0.96237920301445 1108 24-25 1.047610437581021 1108 26-27 0.8731099904640445 1108 28-29 0.9559460335233254 1108 30-31 1.0697525674719017 1108 32-33 1.3236132142900772 1108 34-35 1.3923479998000374 1108 36-37 1.2135035869372146 1108 38-39 1.2171987308513437 1108 40-41 1.3457477465517798 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1.5997298090906895 2311 66-67 1.4738247887127507 2311 68-69 1.3942089506049804 2311 70-71 1.6757578440210033 2311 72-73 1.7215060874029682 2311 74-75 1.7881624432857102 2311 76-77 1.9793214826524874 2311 78-79 2.4366571631381504 2311 80-81 2.3220646872275843 2311 82-83 2.372475358566323 2311 84-85 2.665511158613622 2311 86-87 2.84596027452875 2311 88-89 3.0037243243702747 2311 90-91 3.2762385899311326 2311 92-93 3.3948134133341767 2311 94-95 3.419597092373049 2311 96-97 3.2164057331111415 2311 98-99 3.4456031686368203 2311 100-101 3.3396142085913 2312 1 -0.5335322546152099 2312 2 -0.3173548720267121 2312 3 -0.3470068614332469 2312 4 -0.4316443704927124 2312 5 -0.37001635438685554 2312 6 -0.23329460684016823 2312 7 -0.3595602596330707 2312 8 -0.3910991662921077 2312 9 -0.34476133120727326 2312 10-11 -0.33326664309476683 2312 12-13 -0.3455363455091245 2312 14-15 -0.16365100284379253 2312 16-17 -0.09609179512540322 2312 18-19 -0.1856000909338249 2312 20-21 -0.2694200130810174 2312 22-23 -0.3676719601595053 2312 24-25 -0.37457030446319806 2312 26-27 -0.1851462031310973 2312 28-29 -0.19518610974809292 2312 30-31 -0.06559251666165977 2312 32-33 0.20215952548629446 2312 34-35 -0.018275571670528734 2312 36-37 0.20285724752508116 2312 38-39 0.31239199390142147 2312 40-41 0.4118423090997183 2312 42-43 0.5033273107718159 2312 44-45 0.36958465745798463 2312 46-47 0.29519182867598204 2312 48-49 0.593276607386116 2312 50-51 0.6491134922591613 2312 52-53 0.6689371237412765 2312 54-55 0.8788443772635688 2312 56-57 1.0607642094145753 2312 58-59 1.264991706306244 2312 60-61 1.1280911406639191 2312 62-63 1.3654785801740772 2312 64-65 1.4417746922352634 2312 66-67 1.557613191305979 2312 68-69 1.758658897465974 2312 70-71 1.8246532357658083 2312 72-73 1.78998116022413 2312 74-75 2.0182544650430287 2312 76-77 2.1925781255885823 2312 78-79 2.5018887965831382 2312 80-81 2.5250670132491244 2312 82-83 2.6266275365937624 2312 84-85 2.8008954673193465 2312 86-87 2.97206815847629 2312 88-89 2.9006989285154567 2312 90-91 3.1306706296739577 2312 92-93 3.199482366826068 2312 94-95 3.0447768074942054 2312 96-97 3.133735433505681 2312 98-99 2.892828573226824 2312 100-101 2.4915515706342557 2313 1 -0.4727786887250609 2313 2 -0.5602548453286893 2313 3 -0.4525388053333188 2313 4 -0.44882494569309017 2313 5 -0.3795313024393181 2313 6 -0.5206796207288207 2313 7 -0.386870632666934 2313 8 -0.32371721983584223 2313 9 -0.5060285216096219 2313 10-11 -0.37767988304182154 2313 12-13 -0.4029136908168027 2313 14-15 -0.35444552463675905 2313 16-17 -0.4005569542058538 2313 18-19 -0.4632958811668928 2313 20-21 -0.20633383527218996 2313 22-23 -0.3473461306746586 2313 24-25 -0.24140499825958983 2313 26-27 -0.23699819383791976 2313 28-29 -0.11065744345633988 2313 30-31 -0.11703676607799451 2313 32-33 -0.1348182583506059 2313 34-35 0.06623480363550271 2313 36-37 0.10835749462381017 2313 38-39 0.20809895186055627 2313 40-41 0.3077042291987837 2313 42-43 0.4134512009577449 2313 44-45 0.4638926390306679 2313 46-47 0.4103899336844208 2313 48-49 0.5753638247137047 2313 50-51 0.641291312450825 2313 52-53 0.8391918910424501 2313 54-55 0.9045286703056163 2313 56-57 0.891225744380467 2313 58-59 1.0621627383836305 2313 60-61 1.2634956725112545 2313 62-63 1.2559228466317052 2313 64-65 1.103364871907278 2313 66-67 1.2535700882004868 2313 68-69 1.5722526217265234 2313 70-71 1.6252092362884092 2313 72-73 1.7846666631978323 2313 74-75 1.748079450983962 2313 76-77 2.0987937660855067 2313 78-79 2.254527981327776 2313 80-81 2.220135831662809 2313 82-83 2.253177072786624 2313 84-85 2.5003152681634617 2313 86-87 2.773031218637726 2313 88-89 2.658167274317332 2313 90-91 2.6071569426770296 2313 92-93 2.903846982289334 2313 94-95 3.020495126314035 2313 96-97 3.1619873946742594 2313 98-99 3.2343997802614677 2313 100-101 2.8180848607963185 2314 1 -0.45132803529323695 2314 2 -0.5431965061007489 2314 3 -0.4954155287814892 2314 4 -0.4368326454043512 2314 5 -0.3406922890228046 2314 6 -0.3425648903926515 2314 7 -0.3016844596785404 2314 8 -0.28668068099520383 2314 9 -0.3755608569181277 2314 10-11 -0.3898613132863815 2314 12-13 -0.3560553817383365 2314 14-15 -0.45099503354824577 2314 16-17 -0.3174993339268539 2314 18-19 -0.13668607331178606 2314 20-21 -0.1904261622316099 2314 22-23 -0.16678824948677828 2314 24-25 -0.2993079865360855 2314 26-27 -0.37930230924082053 2314 28-29 -0.1607040591781228 2314 30-31 -0.15724746450210603 2314 32-33 -0.041462506179264835 2314 34-35 -0.2418712297376402 2314 36-37 -0.02490398246839476 2314 38-39 0.15244465034265176 2314 40-41 0.18043020528794074 2314 42-43 0.342933789637776 2314 44-45 0.5987988857693054 2314 46-47 0.5835130710848375 2314 48-49 0.7461957182164909 2314 50-51 0.6127288624583542 2314 52-53 0.7121850378355852 2314 54-55 0.9181294449151167 2314 56-57 0.934989326137714 2314 58-59 1.2454608586800227 2314 60-61 1.0237994864194349 2314 62-63 1.1437341109382082 2314 64-65 1.1445028985083887 2314 66-67 1.3836295754275447 2314 68-69 1.7141543576403429 2314 70-71 1.8221488329812416 2314 72-73 1.8577439965930154 2314 74-75 1.9305565779712204 2314 76-77 1.9649877431487184 2314 78-79 2.2357542531136687 2314 80-81 2.3524775179459567 2314 82-83 2.59784131203428 2314 84-85 2.8623334274222465 2314 86-87 3.131862578726782 2314 88-89 3.178831258454071 2314 90-91 3.0584282067516178 2314 92-93 3.240925150592961 2314 94-95 3.4111404409701613 2314 96-97 3.3855220723648074 2314 98-99 3.324129478650791 2314 100-101 3.0671618231210758 2315 1 -0.8094606222703788 2315 2 -0.657506223723626 2315 3 -0.4675544125563995 2315 4 -0.5599145340708134 2315 5 -0.5401567582577513 2315 6 -0.5037666419371405 2315 7 -0.6845572629653702 2315 8 -0.6208309045991101 2315 9 -0.7175084364262716 2315 10-11 -0.6185789757808138 2315 12-13 -0.5638040318419186 2315 14-15 -0.6705393430095228 2315 16-17 -0.555591231751869 2315 18-19 -0.5297126377817278 2315 20-21 -0.5119127611451688 2315 22-23 -0.5449236562734256 2315 24-25 -0.4525068390367508 2315 26-27 -0.4442492805090552 2315 28-29 -0.6325349778068059 2315 30-31 -0.633650436255067 2315 32-33 -0.5548235093789344 2315 34-35 -0.5827654589159152 2315 36-37 -0.3874982771314919 2315 38-39 -0.27010986403255544 2315 40-41 -0.2949988834941166 2315 42-43 -0.10473252668892741 2315 44-45 -0.2527904614098375 2315 46-47 -0.1996179428580689 2315 48-49 -0.1573862555103389 2315 50-51 0.05348303332949911 2315 52-53 0.3978202057540763 2315 54-55 0.28037627379463004 2315 56-57 0.4656555702213616 2315 58-59 0.6425606496412506 2315 60-61 0.7076258980137453 2315 62-63 0.8542734402896279 2315 64-65 0.8191278754954787 2315 66-67 1.0786489994872817 2315 68-69 1.1713296076362631 2315 70-71 1.3370082969377854 2315 72-73 1.5272740904856796 2315 74-75 1.6014491525505186 2315 76-77 2.0276055750647686 2315 78-79 2.1684292036504793 2315 80-81 2.055880748375664 2315 82-83 2.2722855648663227 2315 84-85 2.410123605629547 2315 86-87 2.6435694490411805 2315 88-89 2.8307423202638304 2315 90-91 3.100148570204091 2315 92-93 3.0538898656873315 2315 94-95 3.014262739138214 2315 96-97 3.1903085242287936 2315 98-99 3.0542410825164925 2315 100-101 2.5919639121105327 2316 1 -0.8147961463105027 2316 2 -0.8298860231010039 2316 3 -0.8375301994813391 2316 4 -0.7470902518155356 2316 5 -0.8802726351169667 2316 6 -0.6565770881495254 2316 7 -0.8390712719587938 2316 8 -0.8268841733642418 2316 9 -0.6747118262567753 2316 10-11 -0.8149253957047122 2316 12-13 -0.7152827589259729 2316 14-15 -1.0943373367833047 2316 16-17 -0.9270500695242703 2316 18-19 -0.7724703340805874 2316 20-21 -0.7565815608684474 2316 22-23 -0.9052401557545764 2316 24-25 -0.8848442655327702 2316 26-27 -0.7881181840026557 2316 28-29 -0.7600384368175384 2316 30-31 -0.47585469083826126 2316 32-33 -0.4256726965114126 2316 34-35 -0.6463334284766233 2316 36-37 -0.5305889032124398 2316 38-39 -0.5639744437627527 2316 40-41 -0.702980550737287 2316 42-43 -0.4259967951081549 2316 44-45 -0.48568997714833273 2316 46-47 -0.2189019276384272 2316 48-49 -0.12883644575592967 2316 50-51 0.11579278773973556 2316 52-53 0.06739388268247026 2316 54-55 0.1075597743134793 2316 56-57 0.28677283068487114 2316 58-59 0.5186675330725912 2316 60-61 0.5393796164502724 2316 62-63 0.8103007664743416 2316 64-65 0.8534066717597462 2316 66-67 1.1837034789061676 2316 68-69 1.3980591129977284 2316 70-71 1.3022236203552886 2316 72-73 1.6630186425437898 2316 74-75 1.9336136285103969 2316 76-77 1.947369497582926 2316 78-79 1.88800720434228 2316 80-81 2.2798819590294173 2316 82-83 2.509824478736956 2316 84-85 2.4060160303960636 2316 86-87 2.939029497467331 2316 88-89 2.8826447761614453 2316 90-91 3.020712388951928 2316 92-93 3.06412421366381 2316 94-95 3.1051871597539176 2316 96-97 3.207806794723435 2316 98-99 3.217272905415145 2316 100-101 3.106958723594449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7163.0 3 2729.0 4 3064.0 5 3637.0 6 4128.0 7 5366.0 8 6759.0 9 8614.0 10 10641.0 11 13121.0 12 15917.0 13 18311.0 14 20890.0 15 23961.0 16 27410.0 17 30683.0 18 30530.0 19 30528.0 20 32475.0 21 35076.0 22 38796.0 23 42899.0 24 47232.0 25 52456.0 26 58122.0 27 64046.0 28 70284.0 29 78092.0 30 85930.0 31 95563.0 32 107587.0 33 122361.0 34 141252.0 35 164271.0 36 185781.0 37 183283.0 38 96957.0 39 6955.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69972917750186 18.319690529245218 14.64337806708894 23.337202226163985 2 15.507470663460632 19.396038971377923 40.283675284491665 24.81281508066978 3 19.424715080716563 23.488282228562213 29.279418163230325 27.807584527490896 4 13.025799456565284 16.09537390803397 36.46854661134276 34.410280024058 5 14.163799762532594 36.985776808026216 33.92989284549555 14.92053058394564 6 33.157003701400726 35.48485915100072 16.99768624415855 14.36045090344 7 29.324852261520558 32.142112882085556 20.088326188309203 18.444708668084687 8 26.776520410222503 34.764764998240544 19.319611925374634 19.139102666162323 9 26.297005844703104 16.91480201740125 19.821940912008973 36.96625122588667 10-11 25.290567078630833 26.285685166349083 27.007805583471672 21.415942171548412 12-13 26.326429820255804 23.403355549515855 27.260290365370338 23.009924264858 14-15 22.849888642554642 25.06314480524172 25.526117979315526 26.56084857288811 16-17 22.63391448181455 28.112807447826555 26.376764200746557 22.876513869612335 18-19 22.6802670593055 26.604551243829526 28.57304380275577 22.1421378941092 20-21 23.068386496123843 25.889756680337676 28.822102414941565 22.219754408596916 22-23 22.749890667089208 25.761014102360956 28.77244176834099 22.716653462208843 24-25 22.600087940354733 25.961118597254984 28.827218503380937 22.61157495900935 26-27 22.199182328892025 26.274048628205087 28.649879154660734 22.87688988824215 28-29 22.342464909449504 26.728501658023916 28.4530290787401 22.47600435378648 30-31 22.454068986779493 26.234655857142275 28.362983801939873 22.948291354138355 32-33 22.10086671159187 26.231652796137446 28.40994195724804 23.257538535022643 34-35 22.978215488334996 26.339113904859644 28.20301483484528 22.47965577196008 36-37 23.041938593230988 25.920662937146616 28.169454003741013 22.867944465881383 38-39 22.508050322532565 26.303356280417646 28.162148692746257 23.026444704303536 40-41 22.992405144151686 26.06484766307545 28.29727037925102 22.64547681352184 42-43 22.652929614125135 26.1326274685958 28.100051358047722 23.114391559231343 44-45 22.61617143714566 26.38514047837266 28.239006877924943 22.75968120655674 46-47 23.092997683688942 26.686651123200473 27.51330395993375 22.707047233176834 48-49 22.837237891855242 26.70137573111459 27.464383709214417 22.99700266781575 50-51 22.95130123584456 26.877388809473118 27.476479414418524 22.694830540263798 52-53 23.239621353689202 26.565157675941553 27.46944706273861 22.725773907630636 54-55 22.706158620890672 26.7918827914215 27.67449552585323 22.827463061834596 56-57 22.77973978496041 26.937837630965454 27.45358896932358 22.828833614750554 58-59 22.805601734263593 26.465591377954347 27.518102630872598 23.210704256909466 60-61 22.414781144601932 26.75891448878318 27.771293530106384 23.0550108365085 62-63 22.638182536138256 26.750950819938048 27.85490561246085 22.755961031462853 64-65 22.357167098773584 26.800572920798594 27.70005147770934 23.142208502718482 66-67 22.280959512412345 26.8158258163654 27.628674667587855 23.2745400036344 68-69 22.874563448744148 26.363526454644926 27.841166438088994 22.920743658521932 70-71 22.736640978539942 26.501599561790286 27.36853898578581 23.393220473883964 72-73 22.6601587075469 26.69453740040222 27.42807692951136 23.217226962539524 74-75 22.65134498174771 27.049359002630503 27.307593064889545 22.991702950732247 76-77 22.86890219637778 26.687964506754085 27.344211385963195 23.098921910904938 78-79 22.943382262555144 26.879745127037292 27.230220796123174 22.946651814284394 80-81 23.052408057662195 26.87424185051079 26.985508921388675 23.08784117043834 82-83 23.03623756137411 26.982328970557408 27.11636285568216 22.865070612386322 84-85 22.972661984282112 27.039174080892792 27.112040649153684 22.876123285671415 86-87 23.24439907281015 27.045537835499328 26.766777690875553 22.94328540081497 88-89 23.15649304575905 27.0784836669701 26.649013310741903 23.116009976528947 90-91 23.017603583335806 27.067515346125383 26.73938599273263 23.17549507780618 92-93 23.17271376934659 26.72764464632434 26.92809380655693 23.171547777772144 94-95 22.97575258254642 26.56315355852716 27.157031497560048 23.304062361366373 96-97 23.32811985094679 26.27323886537048 27.018352809957157 23.380288473725567 98-99 23.08066088502951 26.480418662585354 26.657396174629305 23.78152427775584 100-101 23.592641512210445 25.957246321288903 27.06277518342179 23.387336983078868 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 185.5 2 156.5 3 219.5 4 216.0 5 205.0 6 386.5 7 862.5 8 1427.0 9 1660.5 10 1471.0 11 1248.5 12 1235.5 13 1316.0 14 1453.0 15 1608.5 16 1784.0 17 2003.5 18 2207.5 19 2485.5 20 2941.5 21 3473.5 22 4187.5 23 5139.0 24 6354.0 25 7927.0 26 9924.0 27 12492.0 28 15008.0 29 17779.0 30 20907.5 31 25112.0 32 31052.5 33 34010.0 34 34928.0 35 37794.0 36 41641.0 37 45726.0 38 49417.0 39 52592.5 40 55709.5 41 59229.0 42 62068.0 43 64046.5 44 65823.0 45 68993.0 46 71374.5 47 73205.0 48 74244.5 49 73013.0 50 71665.0 51 69726.5 52 68271.0 53 68095.0 54 68595.5 55 65839.0 56 60855.5 57 56588.5 58 53116.0 59 47737.5 60 39608.5 61 33276.5 62 28238.5 63 22085.5 64 16535.5 65 12721.0 66 9679.5 67 7396.0 68 5635.5 69 4507.5 70 3631.0 71 2658.5 72 2001.5 73 1654.5 74 1274.5 75 951.0 76 705.0 77 499.5 78 335.5 79 210.5 80 136.0 81 90.5 82 61.5 83 37.5 84 24.5 85 15.0 86 10.0 87 8.0 88 4.5 89 5.0 90 4.0 91 1.5 92 2.0 93 2.5 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2990565526078492 2 0.03837047632612573 3 0.04607498412212456 4 0.04911623719949251 5 0.062447063188622066 6 0.060064748278017166 7 0.03223728262010035 8 0.03411272201781059 9 0.042019980018967285 10-11 0.035126473043599914 12-13 0.037255350197757484 14-15 0.031350250472534695 16-17 0.024507431048456794 18-19 0.02914534199144293 20-21 0.04131035430091476 22-23 0.034644941306349986 24-25 0.05474255539262324 26-27 0.047088735147913875 28-29 0.040271259499480704 30-31 0.03968835265965184 32-33 0.035684036107784034 34-35 0.034974410389731515 36-37 0.03436615977425792 38-39 0.02202374103527296 40-41 0.021162052663352037 42-43 0.022505272772522886 44-45 0.009275821885972271 46-47 0.017132392335839495 48-49 0.014369920790563599 50-51 0.006640069218920041 52-53 0.005398224212328125 54-55 0.0075271013664856955 56-57 0.005981131052156983 58-59 0.008135351981959287 60-61 0.007172288507459434 62-63 0.005068755128946596 64-65 0.008743602597432879 66-67 0.002863846647854827 68-69 0.00833810218711715 70-71 0.015180921611195056 72-73 0.01581451600231338 74-75 0.012266387412050763 76-77 0.010847135975945716 78-79 0.006259912584249045 80-81 0.007096257180525234 82-83 0.011936918328669234 84-85 0.004409816962183539 86-87 0.0014192514361050469 88-89 0.022378553894299224 90-91 0.03195850108800829 92-93 0.015256952938129254 94-95 0.02514102543957512 96-97 0.02133945909286517 98-99 0.01540901559199765 100-101 0.10847135975945717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1972871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.62035834086772 #Duplication Level Percentage of deduplicated Percentage of total 1 80.30717474441782 48.682497103492906 2 11.679587992312353 14.160416187353414 3 3.478805989818159 6.326593971034014 4 1.499583465427083 3.6362114814491995 5 0.8154968441987644 2.4717855460587943 6 0.4933145089153104 1.7942941383117181 7 0.3316194642967334 1.4072023530932196 8 0.23049624919197323 1.1178212177794686 9 0.16249500502607991 0.8865454889953859 >10 0.9052859885357145 9.983365889712614 >50 0.06039594894897487 2.4813052379425318 >100 0.030699271551096266 3.374346915805362 >500 0.0027744900479885243 1.1822778501979088 >1k 0.0021859618559909545 2.2120629070263207 >5k 8.407545599965211E-5 0.2832737117472073 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5558 0.2817214100668518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4342 0.22008534769886118 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3078 0.15601628286897623 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2441 0.12372831269758641 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2243 0.11369217754227214 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2220 0.11252636386261444 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2120 0.10745760873366783 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2091 0.10598766974627331 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1994 0.10107097727119513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.068755128946596E-5 0.0 0.0 0.0 0.0 3 5.068755128946596E-5 0.0 0.0 0.0 0.0 4 5.068755128946596E-5 0.0 0.0 0.0 0.0 5 5.068755128946596E-5 0.0 0.0 0.0 0.0 6 5.068755128946596E-5 0.0 0.0 0.0 0.0 7 5.068755128946596E-5 0.0 0.0 0.0 0.0 8 5.068755128946596E-5 0.0 0.0 5.068755128946596E-5 0.0 9 5.068755128946596E-5 0.0 0.0 5.068755128946596E-5 0.0 10-11 5.068755128946596E-5 0.0 0.0 1.0137510257893192E-4 0.0 12-13 7.603132693419894E-5 0.0 0.0 1.5206265386839788E-4 1.0137510257893192E-4 14-15 1.5206265386839788E-4 0.0 0.0 1.7740642951313086E-4 1.0137510257893192E-4 16-17 2.0275020515786384E-4 0.0 0.0 2.0275020515786384E-4 1.5206265386839788E-4 18-19 2.7878153209206277E-4 0.0 0.0 2.0275020515786384E-4 1.5206265386839788E-4 20-21 3.548128590262617E-4 0.0 0.0 2.534377564473298E-4 1.5206265386839788E-4 22-23 3.548128590262617E-4 0.0 0.0 4.561879616051936E-4 2.0275020515786384E-4 24-25 4.561879616051936E-4 0.0 0.0 5.068755128946596E-4 2.0275020515786384E-4 26-27 4.561879616051936E-4 0.0 0.0 8.363445962761884E-4 2.280939808025968E-4 28-29 5.322192885393926E-4 0.0 0.0 0.002483690013183832 2.534377564473298E-4 30-31 6.082506154735915E-4 0.0 0.0 0.008084664430669821 2.534377564473298E-4 32-33 6.082506154735915E-4 0.0 0.0 0.020933958682549443 3.0412530773679577E-4 34-35 6.335943911183245E-4 0.0 0.0 0.04232410532670408 3.0412530773679577E-4 36-37 7.096257180525234E-4 2.534377564473298E-5 0.0 0.0767662964278962 3.0412530773679577E-4 38-39 7.096257180525234E-4 5.068755128946596E-5 0.0 0.13026700681392753 3.0412530773679577E-4 40-41 7.349694936972565E-4 5.068755128946596E-5 0.0 0.18691034537990572 3.0412530773679577E-4 42-43 7.603132693419894E-4 5.068755128946596E-5 0.0 0.2468230310040545 3.0412530773679577E-4 44-45 7.603132693419894E-4 5.068755128946596E-5 0.0 0.3219419820150431 3.2946908338152877E-4 46-47 8.616883719209214E-4 5.068755128946596E-5 0.0 0.4133316369899502 4.055004103157277E-4 48-49 9.123759232103873E-4 5.068755128946596E-5 0.0 0.5104489852605669 4.561879616051936E-4 50-51 9.123759232103873E-4 5.068755128946596E-5 0.0 0.6230767242257603 4.561879616051936E-4 52-53 9.123759232103873E-4 5.068755128946596E-5 0.0 0.7322830534789148 5.068755128946596E-4 54-55 9.123759232103873E-4 5.068755128946596E-5 0.0 0.8482054832779233 5.068755128946596E-4 56-57 9.123759232103873E-4 5.068755128946596E-5 0.0 0.9877989995291127 5.068755128946596E-4 58-59 9.123759232103873E-4 5.068755128946596E-5 0.0 1.1378594951215768 5.068755128946596E-4 60-61 9.123759232103873E-4 5.068755128946596E-5 0.0 1.3013015042544596 5.322192885393926E-4 62-63 9.123759232103873E-4 5.068755128946596E-5 0.0 1.463096167970435 5.575630641841255E-4 64-65 9.123759232103873E-4 5.068755128946596E-5 0.0 1.6357886552136454 6.082506154735915E-4 66-67 9.123759232103873E-4 5.068755128946596E-5 0.0 1.839248486089562 6.082506154735915E-4 68-69 9.123759232103873E-4 5.068755128946596E-5 0.0 2.067697279751185 6.082506154735915E-4 70-71 9.630634744998533E-4 5.068755128946596E-5 0.0 2.303850581208807 6.082506154735915E-4 72-73 9.884072501445862E-4 5.068755128946596E-5 0.0 2.553360052431203 6.335943911183245E-4 74-75 0.0010137510257893193 5.068755128946596E-5 0.0 2.805074432134691 6.589381667630575E-4 76-77 0.0010137510257893193 1.0137510257893192E-4 0.0 3.076861082148808 6.589381667630575E-4 78-79 0.0010390948014340523 1.0137510257893192E-4 0.0 3.382659079078156 6.842819424077905E-4 80-81 0.001267188782236649 1.0137510257893192E-4 0.0 3.710734254799224 7.349694936972565E-4 82-83 0.001267188782236649 1.0137510257893192E-4 0.0 4.045956375252107 7.603132693419894E-4 84-85 0.001267188782236649 1.0137510257893192E-4 0.0 4.389288503911304 7.603132693419894E-4 86-87 0.001267188782236649 1.0137510257893192E-4 0.0 4.746737115604619 7.856570449867224E-4 88-89 0.001267188782236649 1.0137510257893192E-4 0.0 5.126006718128048 8.363445962761883E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3145 0.0 54.538746 1 GTATCAA 5435 0.0 42.254288 1 ATCAACG 7140 0.0 31.816315 3 TCAACGC 7175 0.0 31.661112 4 TATCAAC 7320 0.0 31.163794 2 CAACGCA 7280 0.0 31.008615 5 AACGCAG 7675 0.0 29.592491 6 ACGCAGA 8635 0.0 25.858387 7 TAAGGTG 1295 0.0 25.322088 5 CGCAGAG 8890 0.0 25.06323 8 TACATGG 6190 0.0 24.875654 2 GTACATG 6585 0.0 23.877094 1 TATACCG 100 0.0016490493 23.762392 5 GTAAGGT 1205 0.0 23.269396 4 ACATGGG 6510 0.0 22.557846 3 ATACCGT 255 4.8930815E-10 22.360065 6 GCAGAGT 9855 0.0 22.270464 9 AGGTAAG 1145 0.0 21.998371 2 TGGTATC 1325 0.0 21.16198 2 AGAGTAC 8680 0.0 21.123577 10-11 >>END_MODULE