FastQCFastQC Report
Thu 26 May 2016
SRR938349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972871
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.2833434117081147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.23017217040546492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.1627577271904752No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24050.12190356085116565No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23940.12134599778698152No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23880.12104187247924472No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22640.11475661611935094No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21040.10664660791303637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.051.5836141
GTATCAA58850.042.1012731
ATCAACG76350.032.1301273
TCAACGC77400.031.6328284
TATCAAC78650.031.3209552
CAACGCA79500.030.6776455
AACGCAG83350.029.288686
ACGCAGA95400.025.5394347
CGCAGAG96300.025.202118
TACATGG65300.023.3773822
TAAGGTG11400.023.3536595
GTACATG67250.023.1238141
GCAGAGT107950.022.2623069
ACATGGG67150.022.2308163
GAGTACT63250.021.77608112-13
AGAGTAC94000.021.1451110-11
GTACTTT66050.020.92485414-15
GTAAGGT11850.020.4608464
TGGTATC16400.020.2982312
AGTACTT65850.020.1950312-13
Produced by FastQC (version 0.11.5)