##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938349_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1972871 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.818896927371327 31.0 31.0 34.0 30.0 34.0 2 32.064664136682026 33.0 31.0 34.0 30.0 34.0 3 32.12810974463105 33.0 31.0 34.0 30.0 34.0 4 35.79193520508944 37.0 35.0 37.0 35.0 37.0 5 35.66163170323858 37.0 35.0 37.0 33.0 37.0 6 35.69976800307775 37.0 35.0 37.0 33.0 37.0 7 35.6365890116485 37.0 35.0 37.0 33.0 37.0 8 35.620137860001996 37.0 35.0 37.0 33.0 37.0 9 37.198071237298336 39.0 37.0 39.0 33.0 39.0 10-11 37.13262727263972 39.0 37.0 39.0 33.0 39.0 12-13 37.026683194187555 39.0 37.0 39.0 33.0 39.0 14-15 38.09252581643706 40.0 37.0 41.0 33.0 41.0 16-17 38.00697612768397 40.0 37.0 41.0 33.0 41.0 18-19 38.0037579750526 40.0 37.0 41.0 33.0 41.0 20-21 37.994124299054526 40.0 37.0 41.0 33.0 41.0 22-23 38.029492044842264 40.0 37.0 41.0 33.0 41.0 24-25 37.951704647693646 40.0 37.0 41.0 33.0 41.0 26-27 37.77808863326594 40.0 37.0 41.0 32.0 41.0 28-29 37.647295489669624 40.0 37.0 41.0 32.0 41.0 30-31 37.404684087302215 39.0 36.0 41.0 31.5 41.0 32-33 37.18527998029268 39.0 36.0 41.0 31.0 41.0 34-35 37.008256241791784 39.0 36.0 41.0 31.0 41.0 36-37 36.99696761724411 39.0 36.0 41.0 31.0 41.0 38-39 36.84395761304211 39.0 35.5 41.0 30.0 41.0 40-41 36.67242637759894 39.0 35.0 41.0 30.0 41.0 42-43 36.44076678100089 39.0 35.0 40.0 30.0 41.0 44-45 36.16758064769567 39.0 35.0 40.0 30.0 41.0 46-47 35.81452436575934 38.0 35.0 40.0 28.5 41.0 48-49 35.41306628765895 38.0 34.0 40.0 27.0 41.0 50-51 35.039979299204056 38.0 34.0 40.0 26.5 41.0 52-53 34.51447813871256 38.0 33.0 40.0 24.5 41.0 54-55 34.18009616442231 37.5 33.0 40.0 24.0 41.0 56-57 33.80234313343346 37.0 33.0 40.0 23.0 41.0 58-59 33.32995745793821 36.5 32.0 39.0 22.5 41.0 60-61 32.907510932037624 36.0 31.0 39.0 21.5 40.5 62-63 32.4088815234245 35.5 31.0 39.0 19.5 40.0 64-65 31.898661392457996 35.0 30.5 38.5 18.0 40.0 66-67 31.3984984319806 35.0 30.0 38.0 15.5 40.0 68-69 30.817270617288205 34.0 29.0 37.5 11.5 40.0 70-71 30.239329636859175 34.0 29.0 37.0 8.0 39.0 72-73 29.645393692745245 34.0 28.0 36.0 7.0 39.0 74-75 29.021567553073666 33.5 27.5 36.0 2.0 38.5 76-77 27.74897573130732 31.5 26.0 34.5 2.0 36.5 78-79 28.358834155907815 33.0 27.0 35.0 2.0 37.0 80-81 28.04765136696723 33.0 26.0 35.0 2.0 37.0 82-83 27.539263084104334 33.0 26.0 35.0 2.0 36.0 84-85 26.93530874547804 32.0 25.0 35.0 2.0 36.0 86-87 26.28953084109402 32.0 24.5 35.0 2.0 35.0 88-89 25.52239325328417 31.5 21.0 34.0 2.0 35.0 90-91 24.960585866992822 31.0 19.5 34.0 2.0 35.0 92-93 24.168670683486148 31.0 14.0 34.0 2.0 35.0 94-95 23.37472064823296 30.5 4.5 34.0 2.0 35.0 96-97 22.39163229628293 30.0 2.0 34.0 2.0 35.0 98-99 21.19923705097799 29.5 2.0 34.0 2.0 35.0 100-101 18.05699764454949 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34937542251624976 1101 2 0.3545105350013884 1101 3 0.29102742624071 1101 4 0.24246948542571545 1101 5 0.2342457733983352 1101 6 0.2440282224619139 1101 7 0.2619210604084401 1101 8 0.3364574975188219 1101 9 0.3915257710356457 1101 10-11 0.30918122331326003 1101 12-13 0.3618816269636511 1101 14-15 0.5940767501061899 1101 16-17 0.5414984842157509 1101 18-19 0.6694024647013919 1101 20-21 0.6421056613318896 1101 22-23 0.6118170274938421 1101 24-25 0.6210388913843019 1101 26-27 0.4853360031919678 1101 28-29 0.5320215139636915 1101 30-31 0.5455297618954589 1101 32-33 0.4553728842903766 1101 34-35 0.316376520416938 1101 36-37 0.5052410386914801 1101 38-39 0.6450322072102566 1101 40-41 0.7903228056605087 1101 42-43 0.692326711745288 1101 44-45 0.7223918030579242 1101 46-47 0.8099611555342037 1101 48-49 0.8394287385197359 1101 50-51 0.9361668240371799 1101 52-53 1.166570273699115 1101 54-55 1.1680749893632978 1101 56-57 1.2889337865920965 1101 58-59 1.3464338587320412 1101 60-61 1.4227337261568778 1101 62-63 1.4533193501181358 1101 64-65 1.5687531903400505 1101 66-67 1.6654843780929625 1101 68-69 1.6706240348145904 1101 70-71 1.6799963517074268 1101 72-73 1.915674324232981 1101 74-75 1.8888821451118183 1101 76-77 1.7742570786302458 1101 78-79 2.2068075179341946 1101 80-81 2.442985943148596 1101 82-83 2.6064367186074513 1101 84-85 2.7013082245439293 1101 86-87 2.78981195453407 1101 88-89 3.2472125955100672 1101 90-91 3.238431335083906 1101 92-93 3.3932919355260758 1101 94-95 3.4026784742409006 1101 96-97 3.5808567809232237 1101 98-99 3.91937825928375 1101 100-101 4.387161331795685 1102 1 0.2662927016120058 1102 2 0.2901179142346706 1102 3 0.2724888217194774 1102 4 0.2251543960325577 1102 5 0.21312073042085444 1102 6 0.2451665884581331 1102 7 0.25061019355267433 1102 8 0.3157872099994776 1102 9 0.35751097603822046 1102 10-11 0.4114026218004483 1102 12-13 0.3295100490454814 1102 14-15 0.45926029080586517 1102 16-17 0.5122322978224219 1102 18-19 0.5376394210362747 1102 20-21 0.5285155106958328 1102 22-23 0.5050795017073497 1102 24-25 0.45000329528665617 1102 26-27 0.46762632681238614 1102 28-29 0.3827799321775487 1102 30-31 0.5385365201041665 1102 32-33 0.4225303000545182 1102 34-35 0.5156796137210478 1102 36-37 0.5596862531491027 1102 38-39 0.6624775720890597 1102 40-41 0.7162413149866182 1102 42-43 0.6472061330535226 1102 44-45 0.6176137817410918 1102 46-47 0.7029274880509604 1102 48-49 0.7959810582292022 1102 50-51 0.8627083725157689 1102 52-53 0.8989712643300223 1102 54-55 0.9553203477954852 1102 56-57 1.046735273612903 1102 58-59 1.0105695547523226 1102 60-61 1.034952626578061 1102 62-63 1.355585337740628 1102 64-65 1.2729437632298577 1102 66-67 1.1593955436422192 1102 68-69 1.4187768090109039 1102 70-71 1.5998969394244646 1102 72-73 1.5000321332410635 1102 74-75 1.4883868296571166 1102 76-77 1.680473502474154 1102 78-79 2.019278911049202 1102 80-81 2.2595494638637383 1102 82-83 2.243527089503104 1102 84-85 2.4460124589001246 1102 86-87 2.785191203932385 1102 88-89 2.709295042432881 1102 90-91 2.9385865912296865 1102 92-93 3.160269248788925 1102 94-95 3.447150092987677 1102 96-97 3.6621427745625574 1102 98-99 3.8951216353796774 1102 100-101 4.414502395703199 1103 1 0.2774016301540847 1103 2 0.2004529351025539 1103 3 0.23900969103585368 1103 4 0.21226115209238117 1103 5 0.15286773849697255 1103 6 0.1441910322747617 1103 7 0.2974317774038866 1103 8 0.16992281744800408 1103 9 0.19938411069168893 1103 10-11 0.3383273411685934 1103 12-13 0.4090209953248021 1103 14-15 0.5775227067323883 1103 16-17 0.6013156788288043 1103 18-19 0.5556110509431704 1103 20-21 0.591142633385509 1103 22-23 0.47353064029100267 1103 24-25 0.43570046826847175 1103 26-27 0.5361619073396682 1103 28-29 0.6015013919353649 1103 30-31 0.5530261263365333 1103 32-33 0.5964728779676705 1103 34-35 0.621559167736109 1103 36-37 0.5716081669160573 1103 38-39 0.7618656353842539 1103 40-41 0.6808478277899965 1103 42-43 0.7307857879971849 1103 44-45 0.887038363503045 1103 46-47 0.9672121197476571 1103 48-49 1.0267149518480068 1103 50-51 1.003537682661353 1103 52-53 1.0989812815392739 1103 54-55 1.2084929205722048 1103 56-57 1.2993694213922993 1103 58-59 1.2967511797993154 1103 60-61 1.3490247447456483 1103 62-63 1.4748070840584475 1103 64-65 1.5592825593334716 1103 66-67 1.6017925470357994 1103 68-69 1.6630391524170172 1103 70-71 1.7184696540594757 1103 72-73 1.5103679888763821 1103 74-75 1.6487803494618412 1103 76-77 1.6642221456509851 1103 78-79 1.8773417079948906 1103 80-81 2.253026408500137 1103 82-83 2.524218553458237 1103 84-85 2.6337536121261245 1103 86-87 2.868021915965528 1103 88-89 2.9661977213320228 1103 90-91 3.2327677665457166 1103 92-93 3.3788797105640143 1103 94-95 3.511525923026941 1103 96-97 3.7937250788493735 1103 98-99 4.226358137255428 1103 100-101 4.439788023120926 1104 1 0.29512479986395235 1104 2 0.30696592859929694 1104 3 0.2608433355172082 1104 4 0.16718520892726474 1104 5 0.18377163898710336 1104 6 0.1733219714411689 1104 7 0.1493151584812793 1104 8 0.16147510530912257 1104 9 0.3480352945535259 1104 10-11 0.30633386499216897 1104 12-13 0.3362624196714705 1104 14-15 0.3997060117018023 1104 16-17 0.43284680713752266 1104 18-19 0.468504722581514 1104 20-21 0.4457130892675494 1104 22-23 0.3525361908941349 1104 24-25 0.42616020650761044 1104 26-27 0.4993893165187657 1104 28-29 0.5832113157357028 1104 30-31 0.5472382279251846 1104 32-33 0.5991364091148057 1104 34-35 0.6340946783868873 1104 36-37 0.6130025016181477 1104 38-39 0.7288537101499912 1104 40-41 0.713620846857637 1104 42-43 0.7952910725569851 1104 44-45 0.8639267940754038 1104 46-47 0.8779842600172927 1104 48-49 0.8976264408306918 1104 50-51 0.943316352454616 1104 52-53 1.1643703206532265 1104 54-55 1.273859531254523 1104 56-57 1.2853397558881952 1104 58-59 1.2584185118840878 1104 60-61 1.3233677342003247 1104 62-63 1.4708995803974076 1104 64-65 1.5230451530217444 1104 66-67 1.612657944971943 1104 68-69 1.5447403278898797 1104 70-71 1.4030463527281682 1104 72-73 1.6477057018256644 1104 74-75 1.7445176587489222 1104 76-77 1.7356947419488833 1104 78-79 2.0209653296686376 1104 80-81 2.348677597566361 1104 82-83 2.5819003904186566 1104 84-85 2.6782612857475847 1104 86-87 2.9116661569266853 1104 88-89 2.9248299042231203 1104 90-91 3.0050505372723784 1104 92-93 3.4060423999634395 1104 94-95 3.8565939109141 1104 96-97 4.099919638182737 1104 98-99 4.2736404928702285 1104 100-101 4.699978399613748 1105 1 0.28924532901632816 1105 2 0.26629958856990044 1105 3 0.20155867113700054 1105 4 0.18727340098998013 1105 5 0.06814204565050375 1105 6 0.18067131000070447 1105 7 0.18900158670273726 1105 8 0.21586021064963745 1105 9 0.27258208534235706 1105 10-11 0.28404087136159717 1105 12-13 0.36100519282189225 1105 14-15 0.5202351640780947 1105 16-17 0.4899266699498739 1105 18-19 0.3893768440905774 1105 20-21 0.4055367051421186 1105 22-23 0.41402565684219894 1105 24-25 0.4109715734845807 1105 26-27 0.46974698432865836 1105 28-29 0.4746860830066453 1105 30-31 0.5220054988708043 1105 32-33 0.5604298927012863 1105 34-35 0.6438937964662586 1105 36-37 0.824418474180618 1105 38-39 0.7972025587536109 1105 40-41 0.8035277552358764 1105 42-43 0.7338016066089139 1105 44-45 0.8700512428750145 1105 46-47 1.1016491301789841 1105 48-49 1.2577440302476433 1105 50-51 1.2152418791572117 1105 52-53 1.3512884980172615 1105 54-55 1.3689109766244414 1105 56-57 1.3296807651973523 1105 58-59 1.617066233589135 1105 60-61 1.6401242261160505 1105 62-63 1.722001981181343 1105 64-65 1.7307864563044468 1105 66-67 1.7792429523212832 1105 68-69 1.7598309697321106 1105 70-71 1.9336685967262106 1105 72-73 1.945108935534634 1105 74-75 2.2063010982393685 1105 76-77 2.1144306557166423 1105 78-79 2.305384731922434 1105 80-81 2.5030137073164873 1105 82-83 2.527025329174169 1105 84-85 2.8406816679131914 1105 86-87 2.954782276475928 1105 88-89 3.057852932150606 1105 90-91 3.2652171222797257 1105 92-93 3.5072182941329686 1105 94-95 3.295594400870005 1105 96-97 3.8108456548412377 1105 98-99 4.1462519578383805 1105 100-101 4.6470631619851375 1106 1 0.14580744702365678 1106 2 0.1363767823552422 1106 3 0.09702884091258568 1106 4 0.09745900260532636 1106 5 0.05624188559504972 1106 6 0.005176142181831267 1106 7 0.02832285775414789 1106 8 0.010200322384477545 1106 9 0.17891051089885934 1106 10-11 0.13652357390762404 1106 12-13 0.03892895740656854 1106 14-15 0.13081967165805253 1106 16-17 0.0717896369188935 1106 18-19 0.3027800701402441 1106 20-21 0.3697323457074049 1106 22-23 0.1831554190424498 1106 24-25 0.027679452774954427 1106 26-27 0.13734122424716588 1106 28-29 0.2996869745971438 1106 30-31 0.35490145420941843 1106 32-33 0.25731449599857115 1106 34-35 0.17277702013196716 1106 36-37 0.4060534572715113 1106 38-39 0.5689124282654561 1106 40-41 0.5232107463460665 1106 42-43 0.6429199325155182 1106 44-45 0.09323226063812484 1106 46-47 -0.15533752851562355 1106 48-49 0.4958933564565484 1106 50-51 0.25066135872207695 1106 52-53 0.5886241431829049 1106 54-55 0.5451359322064846 1106 56-57 0.605813162915176 1106 58-59 0.9748350505167593 1106 60-61 0.8527144652466063 1106 62-63 1.0689367169964967 1106 64-65 0.8669184423348391 1106 66-67 1.0276088285081997 1106 68-69 0.7974557295711442 1106 70-71 0.8721203695791111 1106 72-73 1.2414106660339925 1106 74-75 1.5273602024314705 1106 76-77 1.4885633296479988 1106 78-79 1.8041134076508492 1106 80-81 1.7725495321406157 1106 82-83 2.0820471139013783 1106 84-85 2.0755954182013134 1106 86-87 2.369071109191289 1106 88-89 2.4420982767916186 1106 90-91 2.186862474149205 1106 92-93 2.088017575690543 1106 94-95 2.5882072205770044 1106 96-97 2.9932168403597963 1106 98-99 3.2910963986340214 1106 100-101 3.842354271667041 1107 1 0.21583628200269445 1107 2 0.16445912035564447 1107 3 0.1619090536777037 1107 4 0.106222160176074 1107 5 0.05765726888098044 1107 6 0.06410816777813011 1107 7 0.11447470401281379 1107 8 0.2037252400295202 1107 9 0.23864372752637308 1107 10-11 0.2814247444735116 1107 12-13 0.22683310996411876 1107 14-15 0.35592403672054473 1107 16-17 0.31910376536173146 1107 18-19 0.2693623434460477 1107 20-21 0.30488093772276414 1107 22-23 0.23415044998552048 1107 24-25 0.27060573042376745 1107 26-27 0.3547569197397493 1107 28-29 0.37603044465793545 1107 30-31 0.4159789713581361 1107 32-33 0.49349476374829493 1107 34-35 0.5242778072690584 1107 36-37 0.3935754122055002 1107 38-39 0.3331659744475388 1107 40-41 0.5041813559458177 1107 42-43 0.552540171683475 1107 44-45 0.6315661967811792 1107 46-47 0.6461724459526508 1107 48-49 0.6836026121526402 1107 50-51 0.6270526121969411 1107 52-53 0.68749202239146 1107 54-55 0.6550430112368062 1107 56-57 0.6131699967680362 1107 58-59 0.692266077586055 1107 60-61 0.6981902911830389 1107 62-63 0.9578456221105327 1107 64-65 1.1163511159225514 1107 66-67 1.2805194470420815 1107 68-69 1.32569788764388 1107 70-71 1.363187403181513 1107 72-73 1.3945333946086365 1107 74-75 1.4391347604232756 1107 76-77 1.5075733232807487 1107 78-79 1.823030250340281 1107 80-81 2.068544668778646 1107 82-83 2.150977998921288 1107 84-85 2.42895585590389 1107 86-87 2.636669494358131 1107 88-89 2.8899272442261896 1107 90-91 2.9752106384600374 1107 92-93 3.146349695274054 1107 94-95 3.1203418569085173 1107 96-97 3.393700040552325 1107 98-99 3.5025514790887904 1107 100-101 3.8751639619668694 1108 1 0.2802400008343362 1108 2 0.26547536809398764 1108 3 0.20404525042623334 1108 4 0.1982987062570416 1108 5 0.2322451916297581 1108 6 0.2117586228791808 1108 7 0.23066930507221883 1108 8 0.1788285555351905 1108 9 0.31712985745836875 1108 10-11 0.24104258184318894 1108 12-13 0.28505896105929196 1108 14-15 0.4144089349481064 1108 16-17 0.41767869706281147 1108 18-19 0.43772466418782585 1108 20-21 0.4169718796355397 1108 22-23 0.35093068300659525 1108 24-25 0.3632614981087414 1108 26-27 0.4489922682377099 1108 28-29 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1.1297261949812807 2316 74-75 1.060608335588011 2316 76-77 1.0093442384941973 2316 78-79 1.1178247373219499 2316 80-81 1.504040364917305 2316 82-83 1.6783010893557524 2316 84-85 1.6547255289656952 2316 86-87 1.6754890378674006 2316 88-89 1.6532978421087101 2316 90-91 1.9153658588934306 2316 92-93 2.1007908726009035 2316 94-95 2.822528601791923 2316 96-97 2.962893345549073 2316 98-99 3.5853061929572227 2316 100-101 3.8859549117276586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 9.0 4 35.0 5 46.0 6 136.0 7 339.0 8 882.0 9 1559.0 10 2496.0 11 3926.0 12 5522.0 13 7836.0 14 10275.0 15 13195.0 16 16789.0 17 21062.0 18 20835.0 19 18916.0 20 19479.0 21 20611.0 22 22326.0 23 25140.0 24 28709.0 25 33473.0 26 39399.0 27 46774.0 28 55267.0 29 65264.0 30 76941.0 31 90536.0 32 108219.0 33 130618.0 34 162209.0 35 203966.0 36 258649.0 37 289211.0 38 161752.0 39 10462.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27173725168777 19.204214775537555 15.304057187780842 23.219990784993833 2 16.03886919881721 20.20404199504348 39.35491166851847 24.40217713762084 3 19.558550859510984 23.613767990324735 29.71413162373927 27.113549526425007 4 13.577116800845063 16.824465461755988 36.00919674930596 33.58922098809299 5 14.040784846993398 37.1121304222079 34.095939274315896 14.751145456482801 6 33.19406083824031 35.930631044807285 16.67838393893975 14.196924178012651 7 29.33607924694519 32.10949930329961 20.088946515002753 18.46547493475245 8 26.745590563194447 35.1626132676693 19.11974984679688 18.972046322339374 9 26.121373369064678 16.819396706627042 19.751722236273938 37.30750768803434 10-11 25.256086181002203 26.45261651674134 26.987699651928587 21.30359765032787 12-13 26.204729047160203 23.442232158108663 27.385444866897025 22.967593927834105 14-15 22.687342456754646 25.14163369019059 25.51243847164868 26.65858538140608 16-17 22.519769412191675 28.259501001332577 26.476819822482057 22.743909763993692 18-19 22.559280358421812 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28.166626283352986 22.973805253750832 44-45 22.630931731296588 26.442679572713608 28.322702200198496 22.603686495791308 46-47 23.09194219588721 26.682295336236038 27.598143820930925 22.62761864694582 48-49 22.77447435742124 26.75676716825378 27.53636705086141 22.93239142346357 50-51 22.981989192400317 26.829022272616914 27.605631589698465 22.583356945284308 52-53 23.316450999583854 26.53652975790105 27.519462752506374 22.627556490008725 54-55 22.7546359479753 26.824974066975 27.693201353053837 22.727188631995865 56-57 22.825947565755694 26.900745157691507 27.48170052679572 22.79160674975708 58-59 22.73227697097276 26.560530313436608 27.523923257019845 23.18326945857078 60-61 22.372268637939328 26.83414171529715 27.768769473523612 23.024820173239913 62-63 22.615315446372318 26.82253426605186 27.815528739588142 22.74662154798768 64-65 22.358684374193754 26.88224420147085 27.623194826220264 23.135876598115132 66-67 22.29514478660266 26.85021154470976 27.626796305279267 23.227847363408316 68-69 22.852351425615613 26.47183008281856 27.717453837486257 22.95836465407957 70-71 22.60048933762015 26.59233675187075 27.364764345970922 23.442409564538178 72-73 22.566554022031852 26.754688978650908 27.35214314569985 23.326613853617392 74-75 22.552488226549023 27.11799200251816 27.155399415369786 23.174120355563037 76-77 22.61019600369208 26.728432827082965 27.457471877279353 23.203899291945596 78-79 22.645778664697286 26.960911281072104 27.405111636797336 22.988198417433274 80-81 22.821436373690933 26.915039047155137 27.167589771454896 23.095934807699035 82-83 22.731622680453132 27.081591988860804 27.2285111967288 22.958274133957268 84-85 22.5603089211735 27.212830893739408 27.203377329999324 23.023482855087764 86-87 22.804704565654905 27.09986240714256 26.98236738281354 23.113065644388993 88-89 22.641740875410157 27.294151995354987 26.661165373584012 23.40294175565084 90-91 22.451489159381975 27.27968764097568 26.894890559786866 23.37393263985548 92-93 22.57769219442481 26.93151155007779 27.05262979153926 23.438166463958137 94-95 22.305107632480787 26.81199125538365 27.208063781159534 23.674837330976022 96-97 22.593621174420424 26.453985080626154 27.183531006335436 23.768862738617983 98-99 22.248819106773833 26.784062414623154 26.859713584922684 24.107404893680325 100-101 22.335529558819466 26.458818934695895 27.247346421137493 23.958305085347146 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 192.0 2 183.5 3 250.5 4 214.0 5 203.0 6 377.0 7 888.5 8 1472.5 9 1646.5 10 1409.0 11 1216.0 12 1211.0 13 1313.5 14 1485.5 15 1593.5 16 1720.0 17 1917.0 18 2177.0 19 2492.0 20 2814.0 21 3370.5 22 4049.0 23 4889.0 24 6108.0 25 7649.5 26 9634.0 27 12291.0 28 14940.5 29 17682.0 30 20920.5 31 25155.0 32 31767.0 33 35113.5 34 35386.5 35 37829.5 36 41680.5 37 45994.0 38 49612.5 39 52714.5 40 56221.5 41 60032.5 42 62584.5 43 64595.5 44 66958.5 45 70395.5 46 72576.0 47 74336.0 48 75442.0 49 74436.5 50 73036.5 51 70862.0 52 69172.5 53 69343.0 54 70345.5 55 66693.5 56 61322.5 57 56766.5 58 52578.0 59 46976.5 60 38334.5 61 31814.0 62 26902.5 63 20336.5 64 14232.0 65 10670.5 66 8086.0 67 6186.0 68 4847.5 69 3821.0 70 2943.5 71 2072.0 72 1571.0 73 1307.5 74 1033.5 75 770.5 76 566.0 77 368.0 78 241.0 79 169.5 80 91.0 81 49.0 82 36.0 83 22.5 84 14.0 85 8.0 86 4.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0137510257893192E-4 2 0.04683529739146655 3 2.534377564473298E-4 4 0.0 5 0.10345329218180004 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00119115745530245 26-27 0.010973854854169381 28-29 0.0 30-31 0.0 32-33 4.561879616051937E-4 34-35 0.015637109572800248 36-37 0.003066596853012691 38-39 0.012165012309471831 40-41 0.006893506975367371 42-43 0.00552494309055179 44-45 0.002483690013183832 46-47 5.068755128946596E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.534377564473298E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0137510257893192E-4 68-69 2.0275020515786384E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.123759232103874E-4 84-85 0.003548128590262617 86-87 0.001089782352723518 88-89 7.603132693419894E-5 90-91 6.589381667630575E-4 92-93 2.7878153209206277E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.363445962761883E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1972871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.768617347409204 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35670663190967 45.26558602021991 2 12.6424675035709 14.60675735081686 3 3.9442472771611996 6.83561135033658 4 1.6828869148825156 3.888722008992401 5 0.8818291746562207 2.5471026078248444 6 0.5410203885154654 1.8752399880777946 7 0.3547600343733396 1.4345797673107032 8 0.26001592026861936 1.2016608161785867 9 0.18748208817721887 0.9747522910262686 >10 1.038149679717163 10.946803831846333 >50 0.06960106365003946 2.7446173944350534 >100 0.03521493515576157 3.7536446929341034 >500 0.003160343228535374 1.2894418652724264 >1k 0.0023702574214015263 2.3519914586488055 >5k 8.778731190376023E-5 0.28348855607932255 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5590 0.2833434117081147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.23017217040546492 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3211 0.1627577271904752 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2405 0.12190356085116565 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2394 0.12134599778698152 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2388 0.12104187247924472 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2264 0.11475661611935094 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2104 0.10664660791303637 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.068755128946596E-5 0.0 0.0 0.0 0.0 7 5.068755128946596E-5 0.0 0.0 0.0 0.0 8 5.068755128946596E-5 0.0 0.0 1.5206265386839788E-4 0.0 9 5.068755128946596E-5 0.0 0.0 2.0275020515786384E-4 0.0 10-11 1.0137510257893192E-4 0.0 0.0 2.0275020515786384E-4 0.0 12-13 1.0137510257893192E-4 0.0 0.0 2.534377564473298E-4 5.068755128946596E-5 14-15 1.5206265386839788E-4 0.0 0.0 2.534377564473298E-4 5.068755128946596E-5 16-17 1.5206265386839788E-4 0.0 0.0 2.534377564473298E-4 5.068755128946596E-5 18-19 2.0275020515786386E-4 0.0 0.0 2.534377564473298E-4 5.068755128946596E-5 20-21 2.534377564473298E-4 0.0 0.0 3.0412530773679577E-4 7.603132693419894E-5 22-23 2.534377564473298E-4 0.0 0.0 5.068755128946596E-4 1.0137510257893192E-4 24-25 3.548128590262617E-4 5.068755128946596E-5 0.0 5.575630641841255E-4 1.0137510257893192E-4 26-27 3.548128590262617E-4 5.068755128946596E-5 0.0 8.870321475656543E-4 1.0137510257893192E-4 28-29 3.8015663467099467E-4 5.068755128946596E-5 0.0 0.002509033788828565 1.0137510257893192E-4 30-31 4.055004103157277E-4 5.068755128946596E-5 0.0 0.00816069575760402 1.0137510257893192E-4 32-33 4.055004103157277E-4 5.068755128946596E-5 0.0 0.02118739643899677 1.0137510257893192E-4 34-35 4.308441859604607E-4 5.068755128946596E-5 0.0 0.04252685553186194 1.0137510257893192E-4 36-37 4.561879616051936E-4 7.603132693419894E-5 0.0 0.07709576551127773 1.0137510257893192E-4 38-39 4.561879616051936E-4 1.267188782236649E-4 0.0 0.13130610161536158 1.0137510257893192E-4 40-41 4.561879616051936E-4 1.5206265386839788E-4 0.0 0.18815219038649766 1.0137510257893192E-4 42-43 4.8153173724992663E-4 1.5206265386839788E-4 0.0 0.24841968886967267 1.0137510257893192E-4 44-45 5.575630641841255E-4 1.5206265386839788E-4 0.0 0.32427360937435845 1.267188782236649E-4 46-47 6.082506154735915E-4 1.5206265386839788E-4 0.0 0.4163222025160287 1.5206265386839788E-4 48-49 7.096257180525234E-4 1.7740642951313086E-4 0.0 0.5134395507866455 1.5206265386839788E-4 50-51 7.096257180525234E-4 2.280939808025968E-4 0.0 0.6263967588352204 2.0275020515786384E-4 52-53 7.096257180525234E-4 2.534377564473298E-4 0.0 0.7365661515628746 2.0275020515786384E-4 54-55 7.096257180525234E-4 2.534377564473298E-4 0.0 0.8531982070799358 2.534377564473298E-4 56-57 7.096257180525234E-4 2.534377564473298E-4 0.0 0.9931972237414408 2.534377564473298E-4 58-59 7.096257180525234E-4 2.534377564473298E-4 0.0 1.144170095257115 2.534377564473298E-4 60-61 7.096257180525234E-4 3.0412530773679577E-4 0.0 1.309132730928682 2.534377564473298E-4 62-63 7.096257180525234E-4 3.548128590262617E-4 0.0 1.4719664894460915 2.534377564473298E-4 64-65 7.096257180525234E-4 3.548128590262617E-4 0.0 1.6458501341446046 2.534377564473298E-4 66-67 7.096257180525234E-4 4.055004103157277E-4 0.0 1.8502983722706654 2.534377564473298E-4 68-69 7.096257180525234E-4 4.055004103157277E-4 0.0 2.0808000117595116 2.534377564473298E-4 70-71 7.096257180525234E-4 4.055004103157277E-4 0.0 2.3196397534354753 2.7878153209206277E-4 72-73 7.349694936972565E-4 4.055004103157277E-4 0.0 2.573685760498279 3.0412530773679577E-4 74-75 7.856570449867224E-4 4.055004103157277E-4 0.0 2.831837459215529 3.0412530773679577E-4 76-77 8.110008206314553E-4 4.055004103157277E-4 0.0 3.1113286170256442 3.0412530773679577E-4 78-79 8.110008206314553E-4 4.055004103157277E-4 0.0 3.4265544984948333 3.2946908338152877E-4 80-81 9.630634744998533E-4 4.055004103157277E-4 0.0 3.7654514664162027 3.8015663467099467E-4 82-83 9.630634744998533E-4 4.308441859604607E-4 0.0 4.1147900699032025 4.055004103157277E-4 84-85 9.630634744998533E-4 4.561879616051936E-4 0.0 4.472998994865858 4.055004103157277E-4 86-87 9.630634744998533E-4 4.561879616051936E-4 0.0 4.846946404503893 4.561879616051936E-4 88-89 9.630634744998533E-4 4.561879616051936E-4 0.0 5.248036997857437 5.068755128946596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3365 0.0 51.583614 1 GTATCAA 5885 0.0 42.101273 1 ATCAACG 7635 0.0 32.130127 3 TCAACGC 7740 0.0 31.632828 4 TATCAAC 7865 0.0 31.320955 2 CAACGCA 7950 0.0 30.677645 5 AACGCAG 8335 0.0 29.28868 6 ACGCAGA 9540 0.0 25.539434 7 CGCAGAG 9630 0.0 25.20211 8 TACATGG 6530 0.0 23.377382 2 TAAGGTG 1140 0.0 23.353659 5 GTACATG 6725 0.0 23.123814 1 GCAGAGT 10795 0.0 22.262306 9 ACATGGG 6715 0.0 22.230816 3 GAGTACT 6325 0.0 21.776081 12-13 AGAGTAC 9400 0.0 21.14511 10-11 GTACTTT 6605 0.0 20.924854 14-15 GTAAGGT 1185 0.0 20.460846 4 TGGTATC 1640 0.0 20.298231 2 AGTACTT 6585 0.0 20.19503 12-13 >>END_MODULE