FastQCFastQC Report
Thu 26 May 2016
SRR938348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967772
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51570.2620730450479019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.20698536212528684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.1404634276735313No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22940.11657854670154875No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21860.11109010596756129No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21740.11048027921934046No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21510.10931144461858386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.051.9596021
GTATCAA53850.042.070151
ATCAACG69850.031.6360023
TATCAAC70850.031.5248492
TCAACGC70800.031.2115064
CAACGCA72000.030.8225365
AACGCAG76150.029.4480636
ACGCAGA85700.025.886037
CGCAGAG87600.025.2703468
GTACATG65150.024.0850521
TACATGG64450.023.5213812
GTGTAAG9100.023.060961
ACATGGG65050.022.427773
TGGTATC13600.022.0138842
TAAGGTG13100.021.7652655
GCAGAGT97600.021.707189
GTATAGG5300.021.5973991
GTGGTAT15650.021.028141
GAGTACT57900.020.75626812-13
GTAAGGT12200.020.6448174
Produced by FastQC (version 0.11.5)