##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1967772 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.911585793476075 31.0 31.0 34.0 27.0 34.0 2 31.086665020134447 31.0 31.0 34.0 27.0 34.0 3 31.132983394417646 31.0 31.0 34.0 27.0 34.0 4 34.804590674122814 37.0 35.0 37.0 32.0 37.0 5 34.78758260611494 37.0 35.0 37.0 32.0 37.0 6 34.753738237966594 37.0 35.0 37.0 32.0 37.0 7 34.65637177477878 37.0 35.0 37.0 30.0 37.0 8 34.597705933410985 37.0 35.0 37.0 30.0 37.0 9 36.04681030119343 39.0 35.0 39.0 30.0 39.0 10-11 35.8723866382894 38.0 35.0 39.0 30.0 39.0 12-13 35.67110747586611 38.0 35.0 39.0 30.0 39.0 14-15 36.79355789186958 39.0 36.0 41.0 30.0 41.0 16-17 36.71235514073785 39.0 36.0 41.0 30.0 41.0 18-19 36.69833903521343 39.0 36.0 41.0 30.0 41.0 20-21 36.61221777726281 39.0 36.0 41.0 30.0 41.0 22-23 36.44169700554739 39.0 36.0 41.0 29.0 41.0 24-25 36.28292911983705 39.0 35.5 41.0 29.0 41.0 26-27 36.037391272972684 39.0 35.0 40.5 27.0 41.0 28-29 35.776699739603984 38.5 35.0 40.0 27.0 41.0 30-31 35.474495266728056 38.0 34.0 40.0 26.5 41.0 32-33 35.14749574645843 38.0 34.0 40.0 25.5 41.0 34-35 34.81210323147194 38.0 34.0 40.0 25.0 41.0 36-37 34.54243225333016 38.0 33.0 40.0 24.0 41.0 38-39 34.25485981099436 38.0 33.0 40.0 24.0 41.0 40-41 33.9400469160045 38.0 33.0 40.0 22.5 41.0 42-43 33.47567401101347 37.5 32.0 40.0 20.0 41.0 44-45 32.974489676649526 37.0 31.0 40.0 17.0 41.0 46-47 32.44629255828419 37.0 30.5 40.0 14.0 41.0 48-49 31.908711730830603 36.5 30.0 39.0 9.0 40.5 50-51 31.16419026187993 35.5 29.5 38.5 8.0 40.0 52-53 31.131271814010972 36.0 30.0 39.0 7.5 40.0 54-55 31.03766747367073 36.0 29.5 39.0 2.0 40.0 56-57 30.544469582858177 35.0 28.0 39.0 2.0 40.0 58-59 30.03265241095005 35.0 28.0 39.0 2.0 40.0 60-61 29.4151410834182 34.5 26.5 38.0 2.0 40.0 62-63 28.63383791414859 34.0 26.0 38.0 2.0 40.0 64-65 27.91244615738002 34.0 24.0 37.5 2.0 40.0 66-67 27.145895967622266 33.0 22.5 37.0 2.0 39.0 68-69 26.239376309857036 32.0 19.0 36.0 2.0 39.0 70-71 25.342839007771225 31.5 15.5 35.5 2.0 38.5 72-73 24.382986189456908 31.0 7.5 35.0 2.0 38.0 74-75 23.395955425730214 30.0 2.0 34.5 2.0 37.0 76-77 22.39424105028428 29.0 2.0 34.0 2.0 36.0 78-79 21.381577997857477 28.5 2.0 34.0 2.0 36.0 80-81 20.37748783903826 26.5 2.0 33.5 2.0 35.0 82-83 19.329939393385004 25.5 2.0 33.0 2.0 35.0 84-85 18.32249213831684 24.0 2.0 32.0 2.0 35.0 86-87 17.316904600736265 20.5 2.0 32.0 2.0 34.0 88-89 16.208349341285473 14.0 2.0 31.0 2.0 34.0 90-91 15.047961603275176 4.5 2.0 30.5 2.0 34.0 92-93 13.915666550799585 2.0 2.0 30.0 2.0 34.0 94-95 12.708025370825482 2.0 2.0 29.0 2.0 34.0 96-97 11.373346861323364 2.0 2.0 28.0 2.0 33.5 98-99 9.816564368229653 2.0 2.0 22.0 2.0 32.5 100-101 6.937399759728262 2.0 2.0 2.0 2.0 27.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6414979425159153 1101 2 0.5310719696193402 1101 3 0.5623420974231692 1101 4 0.32219455847499745 1101 5 0.38562924929374276 1101 6 0.4565129571934392 1101 7 0.40755882413979094 1101 8 0.3376330519635715 1101 9 0.46184138211828696 1101 10-11 0.5754190220849509 1101 12-13 0.5146409052088288 1101 14-15 0.6259764925153775 1101 16-17 0.692345133880437 1101 18-19 0.5688542173951561 1101 20-21 0.835449353374706 1101 22-23 0.88284978106784 1101 24-25 0.7605059125687532 1101 26-27 0.8719648578356143 1101 28-29 0.9311268496082903 1101 30-31 0.9679051125159717 1101 32-33 0.9451978216260528 1101 34-35 1.0753495966875164 1101 36-37 1.1531553548689502 1101 38-39 1.259917958081374 1101 40-41 1.2829037399141257 1101 42-43 1.4268325085516835 1101 44-45 1.4860682032591157 1101 46-47 1.4991259933447836 1101 48-49 1.2525879963000968 1101 50-51 1.455709251022089 1101 52-53 1.4975167457988903 1101 54-55 1.6760470885300158 1101 56-57 1.763378365680314 1101 58-59 1.526311693122885 1101 60-61 1.8100025809083284 1101 62-63 1.7027511978969336 1101 64-65 1.8037211043469767 1101 66-67 2.2356897058382224 1101 68-69 2.3284286915339862 1101 70-71 2.6875425130489496 1101 72-73 3.0297964970534466 1101 74-75 3.1157135047667666 1101 76-77 3.1602200058824543 1101 78-79 3.303131437328272 1101 80-81 3.7889931775670007 1101 82-83 3.7855432312305446 1101 84-85 3.6294470146052866 1101 86-87 3.8016012069555387 1101 88-89 3.9904160326139895 1101 90-91 4.175583765736368 1101 92-93 4.073636419792846 1101 94-95 4.137260454859765 1101 96-97 3.8529247853322754 1101 98-99 3.442583987374407 1101 100-101 2.6051491139636402 1102 1 0.8411389856687101 1102 2 0.6104178346246769 1102 3 0.5681031531596226 1102 4 0.5014285687624067 1102 5 0.4964493110827206 1102 6 0.6608069952831954 1102 7 0.5695838785210867 1102 8 0.7801883945052026 1102 9 0.6821497354608894 1102 10-11 0.9476892695366246 1102 12-13 0.8948076655359003 1102 14-15 0.8801403919429234 1102 16-17 1.068438070093677 1102 18-19 0.9616118932907369 1102 20-21 0.9025816780437808 1102 22-23 0.8828474955748078 1102 24-25 0.9009513417171249 1102 26-27 0.7843606998515824 1102 28-29 0.7064835881828344 1102 30-31 0.8155111257825922 1102 32-33 1.0128066043724644 1102 34-35 0.9558301417641601 1102 36-37 1.0647225039987163 1102 38-39 1.3574753037904301 1102 40-41 1.4775630804552762 1102 42-43 1.6186351172403164 1102 44-45 1.4509521405451906 1102 46-47 1.5900989680618594 1102 48-49 1.5725051270114783 1102 50-51 1.6471028908982994 1102 52-53 1.6933431666361258 1102 54-55 1.707215298128549 1102 56-57 1.7396577712370167 1102 58-59 2.1534765255693564 1102 60-61 2.0297745155916225 1102 62-63 2.163748452616474 1102 64-65 2.4863830853047837 1102 66-67 2.764224758579335 1102 68-69 2.672259608070469 1102 70-71 2.6724766774054736 1102 72-73 2.7981344865186664 1102 74-75 2.558298289836312 1102 76-77 2.8807902498386717 1102 78-79 3.4331132071603037 1102 80-81 3.775741082307654 1102 82-83 3.8770188799744645 1102 84-85 3.826785221864551 1102 86-87 3.8793281362477607 1102 88-89 4.093659416111656 1102 90-91 4.321166848785806 1102 92-93 4.405678628815975 1102 94-95 3.997645197446028 1102 96-97 4.110133499245013 1102 98-99 3.5597434690783647 1102 100-101 2.5608381094491195 1103 1 0.547535771329656 1103 2 0.4674611708217711 1103 3 0.38534444829236136 1103 4 0.3798500840659287 1103 5 0.4611878100481164 1103 6 0.3323000528347677 1103 7 0.5737285846761182 1103 8 0.6360996143218856 1103 9 0.7723948715996514 1103 10-11 0.7530207685946806 1103 12-13 0.7672419309950058 1103 14-15 0.6027307991662099 1103 16-17 0.6527462230902898 1103 18-19 0.8851297277329593 1103 20-21 0.6969216998671399 1103 22-23 0.9348995188555023 1103 24-25 0.944032792936504 1103 26-27 0.9609415876376701 1103 28-29 1.0572802038557043 1103 30-31 1.012698736602296 1103 32-33 1.16628029598796 1103 34-35 1.1820360766998874 1103 36-37 1.3136299578813109 1103 38-39 1.3902212385529111 1103 40-41 1.4723150004989378 1103 42-43 1.4259270176865186 1103 44-45 1.5673237561064965 1103 46-47 1.896220691178165 1103 48-49 1.835529387060685 1103 50-51 1.700047377822262 1103 52-53 1.8629183460672607 1103 54-55 2.099521447806783 1103 56-57 2.1311663471564053 1103 58-59 2.2391439794414545 1103 60-61 2.3791267838535965 1103 62-63 2.6374063382283595 1103 64-65 2.6088951132210454 1103 66-67 2.8684849862783075 1103 68-69 2.848674471369975 1103 70-71 2.9184475321677787 1103 72-73 3.5535524626788657 1103 74-75 3.477210486900681 1103 76-77 3.4694719996658883 1103 78-79 3.654704711326403 1103 80-81 3.82882271989444 1103 82-83 3.915140595741068 1103 84-85 4.146691308440349 1103 86-87 4.236409795883652 1103 88-89 4.3366224059068585 1103 90-91 4.310921103671847 1103 92-93 4.315630884635953 1103 94-95 4.20298533240228 1103 96-97 4.015016167837301 1103 98-99 3.3596271338175736 1103 100-101 2.4841245595673582 1104 1 0.7655202716548217 1104 2 0.7004907129370466 1104 3 0.6658914230256023 1104 4 0.6148502044985165 1104 5 0.5672889252539406 1104 6 0.7018805861102919 1104 7 0.6281084170339426 1104 8 0.6013307244695341 1104 9 0.8507400374353367 1104 10-11 0.8806653930858559 1104 12-13 0.812922013852635 1104 14-15 0.9821190618056121 1104 16-17 0.9114467252942049 1104 18-19 1.011465400469426 1104 20-21 0.9971055853839133 1104 22-23 1.0418079087921654 1104 24-25 1.1149375578520804 1104 26-27 1.2635713838657168 1104 28-29 1.3132912956975957 1104 30-31 1.2284104480698872 1104 32-33 1.4280412914837797 1104 34-35 1.3965306662656118 1104 36-37 1.490749420992806 1104 38-39 1.4926563103307373 1104 40-41 1.5716214592989388 1104 42-43 1.677012416438842 1104 44-45 1.5678314547600607 1104 46-47 1.6641022969059307 1104 48-49 1.7147561947539138 1104 50-51 1.7798433800623066 1104 52-53 2.0517088565810226 1104 54-55 2.277194900850109 1104 56-57 2.4713850376928157 1104 58-59 2.686610905640361 1104 60-61 2.774363599495377 1104 62-63 2.9611197417738992 1104 64-65 2.8619868437169913 1104 66-67 3.035890431638041 1104 68-69 3.365739017218665 1104 70-71 3.5342257826434853 1104 72-73 3.9612600734166357 1104 74-75 3.8597552125552355 1104 76-77 4.086750764990935 1104 78-79 4.126984139030718 1104 80-81 4.453289429314676 1104 82-83 4.515036045195512 1104 84-85 4.823679424754147 1104 86-87 4.886660370949286 1104 88-89 5.023203731026687 1104 90-91 4.837071912376716 1104 92-93 4.630647173144274 1104 94-95 4.419968736790848 1104 96-97 4.10670413601901 1104 98-99 3.643988810961874 1104 100-101 2.3664434741083893 1105 1 0.6257948994149309 1105 2 0.6442811445662997 1105 3 0.5680195096587717 1105 4 0.4367128637280686 1105 5 0.536672290087445 1105 6 0.5433559468257272 1105 7 0.6098450371418167 1105 8 0.6110496814295416 1105 9 0.5893581976713591 1105 10-11 0.7565083589427388 1105 12-13 0.8949146689333816 1105 14-15 0.9305519280143741 1105 16-17 0.8507142885634664 1105 18-19 0.8690656344158612 1105 20-21 0.9696051148020075 1105 22-23 0.8455149898013943 1105 24-25 0.9147791446158635 1105 26-27 0.8427775217903957 1105 28-29 1.012073737038122 1105 30-31 1.145978183053522 1105 32-33 1.1265677938885403 1105 34-35 1.0875636319688269 1105 36-37 1.2164457861797402 1105 38-39 1.295163407474213 1105 40-41 1.4418642603561196 1105 42-43 1.7620062316480443 1105 44-45 1.6543380947668211 1105 46-47 1.614732732607159 1105 48-49 1.4847883383793565 1105 50-51 1.6571590752174323 1105 52-53 1.6610175909653861 1105 54-55 2.0267545706360615 1105 56-57 2.2530530392755033 1105 58-59 2.3060241860380337 1105 60-61 2.5335310632701784 1105 62-63 2.680893737609729 1105 64-65 2.67366452584179 1105 66-67 2.8207593505673394 1105 68-69 2.854653932168187 1105 70-71 3.1224140326934275 1105 72-73 3.2077775272035716 1105 74-75 3.238078817327896 1105 76-77 3.3609646050929634 1105 78-79 3.664928406766851 1105 80-81 3.7323625078038987 1105 82-83 4.0324444545902445 1105 84-85 4.242899958643729 1105 86-87 4.2480271809033425 1105 88-89 4.049031715092209 1105 90-91 4.0909193996614 1105 92-93 4.285403795864353 1105 94-95 3.9549838722416073 1105 96-97 3.9671225793724734 1105 98-99 3.622041927174477 1105 100-101 2.540236815662502 1106 1 0.3440835462797409 1106 2 0.20172739482441315 1106 3 0.10644235801147062 1106 4 0.21916636018453772 1106 5 0.23792384588885795 1106 6 -0.15094067938220945 1106 7 0.2913911958989175 1106 8 0.0032115681311992716 1106 9 0.2845911463720583 1106 10-11 0.18947912976774717 1106 12-13 0.36660216171086546 1106 14-15 0.6260226277982213 1106 16-17 0.05766737108676523 1106 18-19 0.3134366170382421 1106 20-21 0.47031647362048545 1106 22-23 0.3707007387551897 1106 24-25 0.4191375198003371 1106 26-27 0.5656547171990525 1106 28-29 0.5628428286395462 1106 30-31 0.4702055393940938 1106 32-33 0.5500311262304649 1106 34-35 0.44762533358458967 1106 36-37 0.6414547373659332 1106 38-39 0.7908699165494752 1106 40-41 0.9763357993728761 1106 42-43 0.4410507943470776 1106 44-45 0.8397417885282721 1106 46-47 1.0944845810981505 1106 48-49 0.8681693008639435 1106 50-51 0.8434339704080358 1106 52-53 1.14987218012962 1106 54-55 0.9696756031767002 1106 56-57 1.4536739863773107 1106 58-59 1.506364520556648 1106 60-61 1.8251592887756622 1106 62-63 1.7773671596908365 1106 64-65 1.562720019407358 1106 66-67 1.8934647684503894 1106 68-69 1.9757561405063342 1106 70-71 2.024240595646315 1106 72-73 2.066333866632551 1106 74-75 2.048828244736143 1106 76-77 2.2201629198071977 1106 78-79 2.5010056654495045 1106 80-81 2.409295490414916 1106 82-83 2.7371597405640706 1106 84-85 2.5710871492594762 1106 86-87 2.420665725374164 1106 88-89 2.382245778210862 1106 90-91 2.354905272889871 1106 92-93 2.657725070513271 1106 94-95 2.529122900739532 1106 96-97 2.445701064699156 1106 98-99 2.0736406374458927 1106 100-101 1.207261450842462 1107 1 0.2370843923526067 1107 2 0.10771804281289121 1107 3 0.07210213338235505 1107 4 0.1329234403935402 1107 5 0.19278004579456365 1107 6 0.13734734707210094 1107 7 0.23115532886798462 1107 8 0.1747237780606099 1107 9 0.2177691510609847 1107 10-11 0.2829201274878699 1107 12-13 0.2574450375835795 1107 14-15 0.35129437219379156 1107 16-17 0.2862257210509256 1107 18-19 0.32899693040813105 1107 20-21 0.38620811170797964 1107 22-23 0.4522103329631477 1107 24-25 0.35984218224309217 1107 26-27 0.33846235510569755 1107 28-29 0.4971373588753494 1107 30-31 0.5069862967261827 1107 32-33 0.8723727782038821 1107 34-35 0.5814743838516492 1107 36-37 0.6759951777113358 1107 38-39 0.5984117519463226 1107 40-41 0.5873618726259906 1107 42-43 0.8873396991671143 1107 44-45 0.8414371635452298 1107 46-47 0.8647176709433424 1107 48-49 0.7011595225867033 1107 50-51 0.8934321943008747 1107 52-53 1.1030039414466515 1107 54-55 1.2584488298144372 1107 56-57 1.5032071931226376 1107 58-59 1.5847893395551829 1107 60-61 1.7362796571725774 1107 62-63 1.871026134198857 1107 64-65 1.8767180043330498 1107 66-67 1.8590551141777212 1107 68-69 2.104261530184246 1107 70-71 2.320040748068603 1107 72-73 2.234546553850393 1107 74-75 2.4188050907573384 1107 76-77 2.4219012443245838 1107 78-79 2.9824178966921977 1107 80-81 2.8557111447005 1107 82-83 3.1318809886184233 1107 84-85 3.0396766941239406 1107 86-87 3.106733940806272 1107 88-89 3.359982274952511 1107 90-91 3.3739378757587684 1107 92-93 3.4271710219577276 1107 94-95 3.045931333050472 1107 96-97 2.7591847858697083 1107 98-99 2.2470209891796973 1107 100-101 1.5317521924802273 1108 1 0.33177776435709916 1108 2 0.26852435674865305 1108 3 0.1491426337423043 1108 4 0.1488245491888236 1108 5 0.1104921993884247 1108 6 0.10155358118571911 1108 7 0.17062044992384529 1108 8 0.26019458197635004 1108 9 0.21670353209852777 1108 10-11 0.3472164428069391 1108 12-13 0.18141069773673024 1108 14-15 0.27810819135230247 1108 16-17 0.34445319297252297 1108 18-19 0.31979532169856384 1108 20-21 0.26801122282997625 1108 22-23 0.4995104670811088 1108 24-25 0.43900586771385264 1108 26-27 0.381142049239962 1108 28-29 0.421413217840346 1108 30-31 0.5681679379867717 1108 32-33 0.7739453273299048 1108 34-35 0.65708346892621 1108 36-37 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-0.9537268920925044 2315 38-39 -0.896406639722592 2315 40-41 -0.7512358489831996 2315 42-43 -0.7944026290627662 2315 44-45 -0.8213728363645956 2315 46-47 -0.6871968002627682 2315 48-49 -0.5875058219131368 2315 50-51 -0.48247847180251924 2315 52-53 -0.5130225564599158 2315 54-55 -0.485987282682121 2315 56-57 -0.41843546157931755 2315 58-59 -0.27883509477223 2315 60-61 -0.3551202696203539 2315 62-63 -0.5854205158609531 2315 64-65 -0.27243885418192093 2315 66-67 -0.32407620603059684 2315 68-69 -0.44904019878357815 2315 70-71 -0.4112030622228886 2315 72-73 -0.4395538905946701 2315 74-75 -0.6493406084198767 2315 76-77 -0.5359113904385104 2315 78-79 -0.2646731859885527 2315 80-81 -0.21411131081626067 2315 82-83 -0.2862762373192602 2315 84-85 -0.40717372552181885 2315 86-87 -0.5037768230144479 2315 88-89 -0.44526322192254497 2315 90-91 -0.24426379237273288 2315 92-93 -0.4400372290292136 2315 94-95 -0.5449027766338794 2315 96-97 -0.465513974905269 2315 98-99 -0.6282070892226219 2315 100-101 -0.3343283551807881 2316 1 -0.7332059429689579 2316 2 -0.5168959543735561 2316 3 -0.47438293384357877 2316 4 -0.6338780224244331 2316 5 -0.5067153891154845 2316 6 -0.34847921298175777 2316 7 -0.5417879898046536 2316 8 -0.55347142627582 2316 9 -0.5997604213688774 2316 10-11 -0.753996158316312 2316 12-13 -0.6738169346003104 2316 14-15 -0.8118194906268599 2316 16-17 -0.8303400756330603 2316 18-19 -0.8027566245474844 2316 20-21 -0.7850353600747226 2316 22-23 -0.8341574917358656 2316 24-25 -0.7785086855878163 2316 26-27 -0.9739352567215533 2316 28-29 -0.9857464228380906 2316 30-31 -1.0139483488971734 2316 32-33 -1.0451418035933386 2316 34-35 -0.6473198480780624 2316 36-37 -1.0779595281040741 2316 38-39 -1.1383589549707906 2316 40-41 -0.9622888002071548 2316 42-43 -0.6732946604148893 2316 44-45 -0.9032009955763911 2316 46-47 -0.659225381866726 2316 48-49 -0.7586819592433152 2316 50-51 -0.5244720961281679 2316 52-53 -0.4837232041743107 2316 54-55 -0.7369123224989984 2316 56-57 -0.7944643336649264 2316 58-59 -0.4506927945753567 2316 60-61 -0.6286420711636858 2316 62-63 -0.6363887300056206 2316 64-65 -0.5364690185129604 2316 66-67 -0.47288973272656065 2316 68-69 -0.19026829950112756 2316 70-71 -0.2120791330009304 2316 72-73 -0.15794299574206505 2316 74-75 0.072329969469493 2316 76-77 0.30117130332120823 2316 78-79 0.24828955774992778 2316 80-81 0.2763233124081452 2316 82-83 0.42609594329267964 2316 84-85 0.5258516010959511 2316 86-87 0.6459637330987462 2316 88-89 0.5399596431616587 2316 90-91 0.6507154013815128 2316 92-93 0.6726046170413742 2316 94-95 0.5944310504519432 2316 96-97 0.4411719030108632 2316 98-99 0.6098109156658253 2316 100-101 0.7181928663940402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7554.0 3 3061.0 4 3418.0 5 4002.0 6 4819.0 7 6196.0 8 8045.0 9 10214.0 10 12787.0 11 15879.0 12 19485.0 13 22609.0 14 25567.0 15 29061.0 16 32915.0 17 36681.0 18 37475.0 19 38185.0 20 41822.0 21 45515.0 22 50106.0 23 54766.0 24 60290.0 25 66027.0 26 72085.0 27 78127.0 28 84922.0 29 92386.0 30 101210.0 31 109678.0 32 120231.0 33 132410.0 34 144056.0 35 149660.0 36 138149.0 37 88377.0 38 19743.0 39 259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74211834420951 18.259424317739388 14.613559100685219 23.384898237365885 2 15.5122552794769 19.365969683706684 40.30611129665467 24.815663740161742 3 19.416000951675038 23.5230989630199 29.187090568758013 27.87380951654705 4 13.087246786644155 16.20107897373948 36.37034291556784 34.341331324048525 5 14.113035072187103 37.01961924996199 33.93111675795467 14.93622891989624 6 33.21925156529812 35.437058083568665 16.968319809957503 14.375370541175705 7 29.395882328419336 32.06751891426381 20.093196152176322 18.443402605140534 8 26.732340047595155 34.77181321672978 19.33429825109926 19.161548484575803 9 26.306036825198 17.001012161699823 19.727189123760787 36.965761889341394 10-11 25.29152475421683 26.27542000535325 27.005429763727367 21.42762547670255 12-13 26.33611505568994 23.35281886084517 27.335486493019346 22.97557959044554 14-15 22.883075635830906 25.03379118546951 25.479773834159623 26.60335934453996 16-17 22.624686471398086 28.038841584090473 26.415647240706857 22.92082470380458 18-19 22.684969262547686 26.576516012017944 28.57333844035674 22.16517628507764 20-21 23.033706008771873 25.85971864423582 28.846731136086056 22.259844210906255 22-23 22.792395011243688 25.77884183521609 28.744420413059103 22.68434274048112 24-25 22.671570060454403 25.939112437256505 28.829977777630905 22.559339724658187 26-27 22.19175623869176 26.298080515943 28.61834462865251 22.891818616712733 28-29 22.384209112289984 26.73213244950968 28.43923483625548 22.44442360194486 30-31 22.498847910746825 26.216238955384853 28.38544572572981 22.899467408138516 32-33 22.12929459500951 26.234710362335562 28.40433676192645 23.231658280728475 34-35 23.0049350482982 26.38171693726063 28.127462313575858 22.48588570086531 36-37 22.97765984946941 25.91059415380494 28.216059256517877 22.895686740207772 38-39 22.45090168494281 26.325474705781147 28.161272262574684 23.062351346701362 40-41 23.02135127676152 26.09817348465096 28.281839496177163 22.59863574241036 42-43 22.67503398354203 26.1864252363346 28.061905065650688 23.076635714472683 44-45 22.674839822228275 26.406074062064583 28.178429384937175 22.740656730769963 46-47 23.095995563672332 26.671234471201227 27.482155319247347 22.75061464587909 48-49 22.8407967797821 26.697144442956333 27.429007527277133 23.033051249984435 50-51 23.028929232145398 26.82416292260465 27.398122547724086 22.74878529752587 52-53 23.260362050121454 26.507692999671427 27.48782082541654 22.744124124790577 54-55 22.720233784384728 26.776087923257734 27.686017406772123 22.817660885585415 56-57 22.765038953408887 26.867763735339423 27.461429035371392 22.905768275880295 58-59 22.831972776324022 26.37364759549098 27.524179257091657 23.270200371093345 60-61 22.418336046846406 26.717892643478436 27.76474032226218 23.099030987412974 62-63 22.660545049891667 26.671674553749998 27.873211683691956 22.79456871266638 64-65 22.338103372129602 26.71995076307576 27.709364926009933 23.2325809387847 66-67 22.306621165266137 26.786738865836092 27.585639107488163 23.321000861409612 68-69 22.876143018139636 26.330640191802164 27.74514300670469 23.048073783353516 70-71 22.69914406672625 26.381631729378608 27.371669094026103 23.54755510986904 72-73 22.682200659091787 26.55703319244789 27.344151831002524 23.4166143174578 74-75 22.702861634349066 26.88844055451265 27.166352524828724 23.242345286309558 76-77 22.8793112254427 26.585321663684713 27.229256350853813 23.306110760018772 78-79 22.937538117503557 26.759021142508637 27.08713661313275 23.216304126855054 80-81 23.0956360958399 26.728741449491327 26.830211636366226 23.345410818302547 82-83 23.029831588208253 26.779510436570554 26.960778458863494 23.229879516357705 84-85 23.009418504423625 26.818418837822016 26.905707211163154 23.266455446591205 86-87 23.213151794337794 26.77296165660813 26.60833045565414 23.405556093399937 88-89 23.160012645926265 26.79841031354578 26.40711343517845 23.634463605349513 90-91 23.053477565458913 26.8011033701691 26.480884058446186 23.6645350059258 92-93 23.154942634357177 26.41498022417682 26.663003912472032 23.76707322899397 94-95 22.963650485121057 26.200832187965094 26.77444819004709 24.06106913686676 96-97 23.296318058581285 25.89273279366632 26.64627073690674 24.16467841084566 98-99 23.069465494440262 26.050526088949745 26.239213677194545 24.640794739415455 100-101 23.991242107491274 25.08083954057191 26.59525480165246 24.332663550284355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 166.5 2 172.0 3 227.0 4 202.5 5 188.5 6 318.0 7 699.5 8 1167.5 9 1338.0 10 1222.0 11 1098.5 12 1081.0 13 1122.5 14 1257.5 15 1435.0 16 1614.0 17 1833.0 18 2075.5 19 2376.5 20 2768.5 21 3260.0 22 3949.5 23 4913.5 24 6128.5 25 7647.5 26 9822.0 27 12366.0 28 14781.5 29 17610.5 30 20744.0 31 25024.0 32 30868.0 33 33903.0 34 34817.0 35 37234.5 36 40891.5 37 45103.5 38 48709.0 39 51834.0 40 55022.5 41 58643.0 42 61447.0 43 63221.5 44 65350.0 45 68446.5 46 70757.0 47 72465.5 48 73633.5 49 72948.5 50 71495.5 51 70069.5 52 68941.0 53 68260.0 54 68323.0 55 65713.0 56 61366.5 57 57351.0 58 53514.5 59 48245.5 60 40718.5 61 34337.0 62 28971.0 63 22991.5 64 17281.5 65 13256.5 66 10152.0 67 7695.0 68 5906.0 69 4614.0 70 3693.5 71 2803.5 72 2102.0 73 1591.0 74 1228.0 75 987.5 76 711.0 77 504.0 78 348.0 79 216.5 80 142.5 81 88.0 82 53.5 83 31.0 84 17.0 85 12.5 86 10.0 87 7.0 88 6.0 89 4.0 90 0.5 91 0.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38236137113446067 2 0.02942414060165507 3 0.03628469151913941 4 0.04588946280361749 5 0.06530228095531393 6 0.05356311605206294 7 0.03750434501558107 8 0.03887645519907794 9 0.0353191325011231 10-11 0.037783848941848955 12-13 0.029754463423608022 14-15 0.027594660356992574 16-17 0.02274145581906847 18-19 0.027772526491890322 20-21 0.035039628574855215 22-23 0.03397243176546876 24-25 0.04243377789703279 26-27 0.03725025053715573 28-29 0.03239704599923162 30-31 0.0352429041575955 32-33 0.029983148454190832 34-35 0.03140607753337277 36-37 0.03371833728704342 38-39 0.016084180484324404 40-41 0.012958818399692647 42-43 0.014000605761236568 44-45 0.009782637419375823 46-47 0.01926036146464123 48-49 0.016643188336860164 50-51 0.010773605885234672 52-53 0.00871544060998937 54-55 0.0074703776657051735 56-57 0.008385117788036418 58-59 0.003709779385010052 60-61 0.0076482438006029154 62-63 0.010621149198179464 64-65 0.007267102082964897 66-67 0.0034048660108996365 68-69 0.006072858034365769 70-71 0.01374651128281122 72-73 0.010976881467974948 74-75 0.012323582203629281 76-77 0.005259755703404662 78-79 0.008740850057831904 80-81 0.010112960241328772 82-83 0.01237440109931435 84-85 0.007927747726870797 86-87 0.0020581652752453025 88-89 0.01727842453292353 90-91 0.026756148578188935 92-93 0.011078519259345086 94-95 0.02175048735320962 96-97 0.019616093734436714 98-99 0.011891621590306194 100-101 0.12496366448958517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1967772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.35938280555926 #Duplication Level Percentage of deduplicated Percentage of total 1 80.96851629648643 49.681781866342746 2 11.246865419802777 13.802014413125713 3 3.3658185272782983 6.195736424079384 4 1.4817168339706246 3.6366892170017944 5 0.8038639403385192 2.466229761940822 6 0.4569377666912973 1.6822451606837179 7 0.30684692549144954 1.3179556574757139 8 0.23035951984952086 1.130777436908126 9 0.15460016266050589 0.8537553506238937 >10 0.8919785521166077 9.890560101123492 >50 0.057836574759289675 2.427220708403056 >100 0.029495484139640264 3.279836211352337 >500 0.002915159266571532 1.2304375032623696 >1k 0.0021655468837388477 2.1412045221043123 >5k 8.329026475918645E-5 0.26355566557252164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5157 0.2620730450479019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4073 0.20698536212528684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.1404634276735313 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2294 0.11657854670154875 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2186 0.11109010596756129 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2174 0.11048027921934046 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2151 0.10931144461858386 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.0818895685069204E-5 2 0.0 0.0 0.0 0.0 5.0818895685069204E-5 3 0.0 0.0 0.0 0.0 5.0818895685069204E-5 4 0.0 0.0 0.0 0.0 5.0818895685069204E-5 5 0.0 5.0818895685069204E-5 0.0 0.0 5.0818895685069204E-5 6 0.0 5.0818895685069204E-5 0.0 0.0 5.0818895685069204E-5 7 0.0 5.0818895685069204E-5 0.0 5.0818895685069204E-5 5.0818895685069204E-5 8 0.0 5.0818895685069204E-5 0.0 5.0818895685069204E-5 5.0818895685069204E-5 9 0.0 5.0818895685069204E-5 0.0 5.0818895685069204E-5 5.0818895685069204E-5 10-11 0.0 5.0818895685069204E-5 0.0 5.0818895685069204E-5 5.0818895685069204E-5 12-13 0.0 5.0818895685069204E-5 0.0 5.0818895685069204E-5 1.5245668705520763E-4 14-15 1.0163779137013841E-4 5.0818895685069204E-5 0.0 5.0818895685069204E-5 1.7786613489774222E-4 16-17 1.0163779137013841E-4 5.0818895685069204E-5 0.0 1.0163779137013841E-4 2.0327558274027682E-4 18-19 1.0163779137013841E-4 5.0818895685069204E-5 0.0 1.2704723921267303E-4 2.54094478425346E-4 20-21 1.0163779137013841E-4 5.0818895685069204E-5 0.0 1.5245668705520763E-4 2.54094478425346E-4 22-23 1.0163779137013841E-4 5.0818895685069204E-5 0.0 2.286850305828114E-4 2.54094478425346E-4 24-25 1.0163779137013841E-4 1.2704723921267303E-4 0.0 4.573700611656228E-4 2.54094478425346E-4 26-27 1.0163779137013841E-4 1.7786613489774222E-4 0.0 9.147401223312457E-4 2.54094478425346E-4 28-29 1.5245668705520763E-4 2.0327558274027682E-4 0.0 0.002693401471308668 2.54094478425346E-4 30-31 2.0327558274027682E-4 2.0327558274027682E-4 0.0 0.008385117788036418 2.54094478425346E-4 32-33 2.0327558274027682E-4 2.0327558274027682E-4 0.0 0.021115251157146256 2.54094478425346E-4 34-35 2.286850305828114E-4 2.5409447842534606E-4 0.0 0.04040102206963002 2.54094478425346E-4 36-37 2.54094478425346E-4 3.0491337411041525E-4 0.0 0.07566933567506805 2.54094478425346E-4 38-39 3.0491337411041525E-4 3.3032282195294985E-4 0.0 0.12811443602205946 2.54094478425346E-4 40-41 3.0491337411041525E-4 3.5573226979548444E-4 0.0 0.1901897171013715 2.54094478425346E-4 42-43 3.3032282195294985E-4 4.0655116548055364E-4 0.0 0.24919045499173686 2.54094478425346E-4 44-45 4.0655116548055364E-4 4.0655116548055364E-4 0.0 0.32424996391858407 2.54094478425346E-4 46-47 5.08188956850692E-4 4.573700611656228E-4 0.0 0.4194083460888761 2.795039262678806E-4 48-49 5.08188956850692E-4 4.573700611656228E-4 0.0 0.522392838194669 3.0491337411041525E-4 50-51 5.08188956850692E-4 4.573700611656228E-4 0.0 0.633711629192813 3.0491337411041525E-4 52-53 5.08188956850692E-4 4.573700611656228E-4 0.0 0.7388051054695361 3.0491337411041525E-4 54-55 5.08188956850692E-4 4.573700611656228E-4 0.0 0.8544943214965961 3.0491337411041525E-4 56-57 5.08188956850692E-4 4.573700611656228E-4 0.0 0.9949069302744423 3.0491337411041525E-4 58-59 5.08188956850692E-4 4.573700611656228E-4 0.0 1.144797263097554 3.0491337411041525E-4 60-61 5.08188956850692E-4 5.335984046932266E-4 0.0 1.310339815791667 3.3032282195294985E-4 62-63 5.08188956850692E-4 5.590078525357612E-4 0.0 1.4710291639478559 3.8114171763801904E-4 64-65 5.335984046932266E-4 5.590078525357612E-4 0.0 1.6399003543093407 4.3196061332308823E-4 66-67 6.098267482208305E-4 5.590078525357612E-4 0.0 1.8436841260064682 4.573700611656228E-4 68-69 6.098267482208305E-4 5.590078525357612E-4 0.0 2.069828211805026 4.573700611656228E-4 70-71 6.352361960633652E-4 5.590078525357612E-4 0.0 2.306466399562551 4.573700611656228E-4 72-73 6.606456439058997E-4 5.590078525357612E-4 0.0 2.5494315398328666 4.573700611656228E-4 74-75 6.606456439058997E-4 5.590078525357612E-4 0.0 2.792803231268663 4.827795090081574E-4 76-77 6.606456439058997E-4 6.606456439058997E-4 0.0 3.0582557328796223 5.08188956850692E-4 78-79 6.606456439058997E-4 6.860550917484342E-4 0.0 3.35747739067331 5.08188956850692E-4 80-81 7.622834352760381E-4 7.622834352760381E-4 0.0 3.669048040118469 5.335984046932266E-4 82-83 7.622834352760381E-4 8.131023309611073E-4 0.0 3.9931455473499975 6.098267482208305E-4 84-85 8.131023309611073E-4 8.639212266461765E-4 0.0 4.318056156912488 6.098267482208305E-4 86-87 8.639212266461765E-4 8.639212266461765E-4 0.0 4.6534354589861024 6.098267482208305E-4 88-89 8.639212266461765E-4 8.639212266461765E-4 0.0 5.001239981054716 6.098267482208305E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3075 0.0 51.959602 1 GTATCAA 5385 0.0 42.07015 1 ATCAACG 6985 0.0 31.636002 3 TATCAAC 7085 0.0 31.524849 2 TCAACGC 7080 0.0 31.211506 4 CAACGCA 7200 0.0 30.822536 5 AACGCAG 7615 0.0 29.448063 6 ACGCAGA 8570 0.0 25.88603 7 CGCAGAG 8760 0.0 25.270346 8 GTACATG 6515 0.0 24.085052 1 TACATGG 6445 0.0 23.521381 2 GTGTAAG 910 0.0 23.06096 1 ACATGGG 6505 0.0 22.42777 3 TGGTATC 1360 0.0 22.013884 2 TAAGGTG 1310 0.0 21.765265 5 GCAGAGT 9760 0.0 21.70718 9 GTATAGG 530 0.0 21.597399 1 GTGGTAT 1565 0.0 21.02814 1 GAGTACT 5790 0.0 20.756268 12-13 GTAAGGT 1220 0.0 20.644817 4 >>END_MODULE