FastQCFastQC Report
Thu 26 May 2016
SRR938348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967772
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.26547791105880153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.23102269978432463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.16074016705187388No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24830.12618331798602683No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24070.12232108191396157No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23460.11922112927717236No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22760.1156638065792175No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21100.10722786989549601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.049.152731
GTATCAA55400.042.106641
ATCAACG72950.031.5772233
TCAACGC74250.031.0243554
CAACGCA75550.030.4276475
TATCAAC76150.030.376562
AACGCAG79850.028.7296096
TAAGGTG14450.027.281475
TGGTATC15850.025.1726862
AAGGTAA12600.024.885841
ACGCAGA92000.024.8321727
AGGTAAG13050.024.3861852
CGCAGAG93800.024.3050148
TACATGG63500.023.4874272
GTAAGGT13950.023.1521954
GTACATG66700.022.935541
GTATAGG6350.022.4453531
ACATGGG66450.022.2291533
TAAGGTA7950.022.105084
GGTAAGG15000.021.8481833
Produced by FastQC (version 0.11.5)