##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938348_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1967772 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.833471560729595 31.0 31.0 34.0 30.0 34.0 2 32.09687555265549 33.0 31.0 34.0 30.0 34.0 3 32.151388982056865 33.0 31.0 34.0 30.0 34.0 4 35.797527355811546 37.0 35.0 37.0 35.0 37.0 5 35.69012314434802 37.0 35.0 37.0 33.0 37.0 6 35.72353910920574 37.0 35.0 37.0 33.0 37.0 7 35.64418794453829 37.0 35.0 37.0 33.0 37.0 8 35.61365544382174 37.0 35.0 37.0 33.0 37.0 9 37.17046487093017 39.0 37.0 39.0 33.0 39.0 10-11 37.09865395990999 39.0 37.0 39.0 33.0 39.0 12-13 36.983586767166116 39.0 37.0 39.0 33.0 39.0 14-15 38.00744191908412 40.0 37.0 41.0 33.0 41.0 16-17 38.09258770833206 40.0 37.0 41.0 33.0 41.0 18-19 38.228417977285986 40.0 38.0 41.0 33.0 41.0 20-21 38.182908639822095 40.0 37.5 41.0 33.0 41.0 22-23 38.05459346916208 40.0 37.0 41.0 33.0 41.0 24-25 37.93330121579126 40.0 37.0 41.0 33.0 41.0 26-27 37.71497180567667 40.0 37.0 41.0 32.0 41.0 28-29 37.57798337409009 39.5 37.0 41.0 32.0 41.0 30-31 37.30936587165586 39.0 36.0 41.0 31.0 41.0 32-33 37.193229957535735 39.0 36.0 41.0 31.0 41.0 34-35 37.053657385103556 39.0 36.0 41.0 31.0 41.0 36-37 36.9105584894998 39.0 36.0 41.0 30.5 41.0 38-39 36.69314381950754 39.0 35.0 40.5 30.0 41.0 40-41 36.468324074130535 39.0 35.0 40.0 30.0 41.0 42-43 36.19547539044157 38.5 35.0 40.0 30.0 41.0 44-45 35.90078169625343 38.0 35.0 40.0 29.0 41.0 46-47 35.479575885824175 38.0 34.5 40.0 27.5 41.0 48-49 35.05070201222499 38.0 34.0 40.0 26.0 41.0 50-51 34.64568786424444 38.0 33.0 40.0 25.0 41.0 52-53 34.031484846821684 37.5 33.0 40.0 23.5 41.0 54-55 33.59043705266667 37.0 32.0 40.0 23.0 41.0 56-57 33.176047834810134 36.5 32.0 39.0 22.0 40.5 58-59 32.60944281146393 36.0 31.0 39.0 19.5 40.0 60-61 32.0968211764371 35.5 31.0 39.0 18.0 40.0 62-63 31.5621189853296 35.0 30.0 38.5 13.5 40.0 64-65 30.88423328515702 34.5 29.0 38.0 8.0 40.0 66-67 30.207203629282255 34.0 28.0 37.5 7.5 40.0 68-69 29.45526463431739 34.0 27.5 37.0 3.5 39.0 70-71 28.661288248841835 33.5 26.0 36.0 2.0 39.0 72-73 28.067281676942248 33.0 26.0 36.0 2.0 38.5 74-75 27.35753735697022 32.0 25.0 35.0 2.0 38.0 76-77 26.207028049997664 30.5 23.0 34.0 2.0 36.0 78-79 26.673084330908257 32.0 24.5 35.0 2.0 36.0 80-81 26.291486767775943 32.0 23.5 35.0 2.0 36.0 82-83 25.597828661044062 31.0 21.0 34.5 2.0 36.0 84-85 24.79941781873103 31.0 18.5 34.0 2.0 35.0 86-87 23.87773888438295 30.5 11.5 34.0 2.0 35.0 88-89 22.882170292086684 29.5 4.5 34.0 2.0 35.0 90-91 21.993800348820898 29.0 2.0 34.0 2.0 35.0 92-93 20.90475852893526 28.5 2.0 33.5 2.0 35.0 94-95 19.725895835493134 26.5 2.0 33.0 2.0 34.5 96-97 18.33875215218023 24.5 2.0 32.5 2.0 34.0 98-99 16.564348664377782 11.0 2.0 32.0 2.0 34.0 100-101 12.379658314072973 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.328260035259472 1101 2 0.21691568334852462 1101 3 0.28219514354718456 1101 4 0.1434853251278625 1101 5 0.1292845771087343 1101 6 0.15719700930797842 1101 7 0.19291725058999987 1101 8 0.2271680935065703 1101 9 0.32060024553959465 1101 10-11 0.29443588462799397 1101 12-13 0.251724939909721 1101 14-15 0.3708877675784237 1101 16-17 0.3381382747276618 1101 18-19 0.45321279309833784 1101 20-21 0.30153418210064586 1101 22-23 0.4473090811416398 1101 24-25 0.26695271552770805 1101 26-27 0.3437184354159015 1101 28-29 0.42227889619387327 1101 30-31 0.36751831951819725 1101 32-33 0.43828735235350536 1101 34-35 0.3693858599192623 1101 36-37 0.5437602883438828 1101 38-39 0.5703685824609792 1101 40-41 0.5507067170149966 1101 42-43 0.5562252067913676 1101 44-45 0.5382261803548047 1101 46-47 0.6401345868758312 1101 48-49 0.6701246284366675 1101 50-51 0.7558764641908979 1101 52-53 0.9396321504838099 1101 54-55 0.9183926373987177 1101 56-57 0.9828570719008312 1101 58-59 0.9301383117530833 1101 60-61 0.9583907844564905 1101 62-63 0.9067858304360605 1101 64-65 1.0942759619365212 1101 66-67 1.1968035617079664 1101 68-69 1.4412248304149635 1101 70-71 1.7740266416840633 1101 72-73 1.6327530827142311 1101 74-75 1.5436412718317456 1101 76-77 1.5692329450745603 1101 78-79 1.6372225576977328 1101 80-81 1.7465766100385345 1101 82-83 1.900157504516887 1101 84-85 1.9918957517757825 1101 86-87 2.084755242215323 1101 88-89 1.8987945972060558 1101 90-91 2.2715153251189726 1101 92-93 2.7701223622257025 1101 94-95 2.8933050889253735 1101 96-97 2.9029147687681345 1101 98-99 2.9132618973390016 1101 100-101 2.6492178307580083 1102 1 0.23924492142847598 1102 2 0.19664766223594654 1102 3 0.26107960784106155 1102 4 0.2674187393049081 1102 5 0.23329897840366698 1102 6 0.24263035223142282 1102 7 0.3161687811981295 1102 8 0.2840120696191377 1102 9 0.23210138425289273 1102 10-11 0.3145007657420251 1102 12-13 0.2985497178808032 1102 14-15 0.5370168952460688 1102 16-17 0.42418049988368267 1102 18-19 0.4335223295196897 1102 20-21 0.41047435864826554 1102 22-23 0.48623492630352416 1102 24-25 0.3404206957702911 1102 26-27 0.5638439493157321 1102 28-29 0.5688826535444278 1102 30-31 0.5419203942081197 1102 32-33 0.5485263611486246 1102 34-35 0.48034090023804765 1102 36-37 0.5320248193741577 1102 38-39 0.7192000233164535 1102 40-41 0.5867876611118348 1102 42-43 0.5742608389721795 1102 44-45 0.5996090111933725 1102 46-47 0.7049016951356037 1102 48-49 0.9675448445636405 1102 50-51 1.0325598642516667 1102 52-53 1.0371475506736374 1102 54-55 0.8179847441124224 1102 56-57 1.0609334127458183 1102 58-59 1.2361143382755628 1102 60-61 1.2176818514053096 1102 62-63 1.2341454675535424 1102 64-65 1.2893144254398834 1102 66-67 1.3857451364395565 1102 68-69 1.6202879664878402 1102 70-71 1.6049708729652004 1102 72-73 1.6496540348237403 1102 74-75 1.704051961485991 1102 76-77 1.7460601967557423 1102 78-79 1.894014774115103 1102 80-81 2.1028088806624226 1102 82-83 2.157238096378922 1102 84-85 2.2922693626665627 1102 86-87 2.229700538267334 1102 88-89 2.097167998369237 1102 90-91 2.3095193099432976 1102 92-93 2.5959988488044807 1102 94-95 2.7663060219442954 1102 96-97 3.1165110323903455 1102 98-99 2.7590233832859106 1102 100-101 2.616251744785828 1103 1 0.2314820626679328 1103 2 0.2883462016666982 1103 3 0.2586599814105952 1103 4 0.17172248773096044 1103 5 0.21266338154629238 1103 6 0.27242000117559684 1103 7 0.26107306315994094 1103 8 0.29405359805237197 1103 9 0.31861969721311567 1103 10-11 0.3132765156149091 1103 12-13 0.44775034607020814 1103 14-15 0.5409857335655133 1103 16-17 0.4795548503210796 1103 18-19 0.3769770142844635 1103 20-21 0.36761024857494107 1103 22-23 0.4436882970468332 1103 24-25 0.40201906140686816 1103 26-27 0.4135974324832219 1103 28-29 0.4189479740084323 1103 30-31 0.4714769263988785 1103 32-33 0.45030273128374887 1103 34-35 0.3794875212525781 1103 36-37 0.5251916977384425 1103 38-39 0.4912057487332149 1103 40-41 0.5105254615931898 1103 42-43 0.4940992295557436 1103 44-45 0.5991127751587868 1103 46-47 0.709533975178104 1103 48-49 0.7609943714135454 1103 50-51 0.8070585818601543 1103 52-53 0.9706771210127627 1103 54-55 1.2015392378112395 1103 56-57 1.3563529218435662 1103 58-59 1.3887552825650928 1103 60-61 1.459447496227348 1103 62-63 1.4347516078608997 1103 64-65 1.7427435726200002 1103 66-67 1.634293111216433 1103 68-69 1.7490027479694312 1103 70-71 1.8348447022300824 1103 72-73 1.7106990730014964 1103 74-75 1.672922042141078 1103 76-77 1.690786248478208 1103 78-79 1.6070566078234023 1103 80-81 1.6816464940151512 1103 82-83 2.1336708876980843 1103 84-85 2.0569711918757925 1103 86-87 2.101438682084698 1103 88-89 2.0884792085788106 1103 90-91 2.199692209024821 1103 92-93 2.3456058366898134 1103 94-95 2.193368905720064 1103 96-97 2.4143462305789605 1103 98-99 2.448623215712736 1103 100-101 2.1393047369145606 1104 1 0.2586110098462022 1104 2 0.25855870497859 1104 3 0.29828456290732674 1104 4 0.20160482508673994 1104 5 0.15268493898059887 1104 6 0.15551307148414395 1104 7 0.13595789949204828 1104 8 0.2302604530756156 1104 9 0.2844243675891036 1104 10-11 0.32796891571607745 1104 12-13 0.39509533426780763 1104 14-15 0.4644308735081921 1104 16-17 0.4537815225276489 1104 18-19 0.46216500956350615 1104 20-21 0.4139210971430032 1104 22-23 0.42459596739873007 1104 24-25 0.42373832700891256 1104 26-27 0.5241681023293054 1104 28-29 0.5568741548500142 1104 30-31 0.5198396452851242 1104 32-33 0.6241291156564159 1104 34-35 0.6724296443587718 1104 36-37 0.6738570787871723 1104 38-39 0.7862012475650175 1104 40-41 0.6624120381762779 1104 42-43 0.5911291359796138 1104 44-45 0.7119223621954234 1104 46-47 0.5264053019839849 1104 48-49 0.6525034219229795 1104 50-51 0.7459514600787216 1104 52-53 0.7330436666446758 1104 54-55 1.0174000598841175 1104 56-57 1.09744580247078 1104 58-59 1.2442158469122262 1104 60-61 1.1050121143355938 1104 62-63 1.2178483664176376 1104 64-65 1.3252342541480502 1104 66-67 1.252230868641103 1104 68-69 1.3676962199206777 1104 70-71 1.6000451773366642 1104 72-73 1.9413907173881348 1104 74-75 1.902341617254887 1104 76-77 1.9152703453541875 1104 78-79 1.9769845138618898 1104 80-81 2.320067953899052 1104 82-83 2.2687239984719874 1104 84-85 2.5499288959488275 1104 86-87 2.7184152368694896 1104 88-89 2.552343408785955 1104 90-91 2.9934735967696007 1104 92-93 3.1672944672507874 1104 94-95 2.9040589543748325 1104 96-97 2.9980536576570245 1104 98-99 2.934957503474376 1104 100-101 2.222251180540063 1105 1 0.22118822369398217 1105 2 0.22912620755239033 1105 3 0.2006834742304946 1105 4 0.1605105396797697 1105 5 0.11601220276768487 1105 6 0.16918683721502248 1105 7 0.1810141964541998 1105 8 0.21164461693800263 1105 9 0.3456201834436854 1105 10-11 0.2921055323666124 1105 12-13 0.26713907149487426 1105 14-15 0.4121441815645497 1105 16-17 0.31074911191900156 1105 18-19 0.3161232756708543 1105 20-21 0.2781927900085961 1105 22-23 0.4509595985239798 1105 24-25 0.2311507603591494 1105 26-27 0.22791758372883208 1105 28-29 0.307929122658372 1105 30-31 0.48345886192950616 1105 32-33 0.5154803964075043 1105 34-35 0.582888711415734 1105 36-37 0.5707105664713765 1105 38-39 0.5642289619582925 1105 40-41 0.6962689659102637 1105 42-43 0.6616407929657413 1105 44-45 0.8125531820451428 1105 46-47 0.6798819899324826 1105 48-49 0.7519234537304698 1105 50-51 0.8843901749483436 1105 52-53 0.8967867231606022 1105 54-55 0.8619385246730715 1105 56-57 1.0885971819480318 1105 58-59 1.027790905686949 1105 60-61 1.099254208559536 1105 62-63 1.2453958951903594 1105 64-65 1.3860442408565135 1105 66-67 1.5484821075200053 1105 68-69 1.610967618912678 1105 70-71 1.7134076387297412 1105 72-73 1.611816277092494 1105 74-75 1.6334847279357554 1105 76-77 1.5368883980286299 1105 78-79 1.407543670809602 1105 80-81 1.529893223955444 1105 82-83 1.7117701423893799 1105 84-85 1.9661595540720285 1105 86-87 2.027461981979279 1105 88-89 1.927253133889291 1105 90-91 2.101495913876498 1105 92-93 2.4528260423364507 1105 94-95 2.344482804618387 1105 96-97 2.364095794905598 1105 98-99 2.286139576416492 1105 100-101 2.180516327515278 1106 1 0.028465869598321092 1106 2 0.08146857668983642 1106 3 0.004409535727511127 1106 4 0.004958815084563639 1106 5 0.14613460195080563 1106 6 0.06622735719842865 1106 7 0.054846516582720994 1106 8 0.08849050308648998 1106 9 0.009649960109221922 1106 10-11 0.22483168479630677 1106 12-13 0.12805018858396977 1106 14-15 0.020852785880443037 1106 16-17 -0.020975218068137735 1106 18-19 0.13954052598011657 1106 20-21 0.1027800882280232 1106 22-23 0.244087976531155 1106 24-25 0.18903377167080038 1106 26-27 0.052910763777440195 1106 28-29 0.05916405482827258 1106 30-31 0.23382638153213975 1106 32-33 0.01222978971599531 1106 34-35 -0.08256804535638196 1106 36-37 0.1324791977260773 1106 38-39 0.10347470375114654 1106 40-41 0.2312041668999072 1106 42-43 0.34234203552299647 1106 44-45 0.23865711431545122 1106 46-47 -0.03092033945981143 1106 48-49 0.1496291557781646 1106 50-51 0.3833624461626499 1106 52-53 0.27052124329177474 1106 54-55 0.1211658241807001 1106 56-57 0.07888538172714021 1106 58-59 0.448288743194027 1106 60-61 0.5881130601933933 1106 62-63 0.423793272919049 1106 64-65 0.7068559382098059 1106 66-67 0.5561794172386243 1106 68-69 0.4453455730409246 1106 70-71 0.42851672006334596 1106 72-73 0.7855959794016165 1106 74-75 0.7839870836606302 1106 76-77 0.748855092038248 1106 78-79 0.932542107904073 1106 80-81 1.1533944922769592 1106 82-83 1.3813299997766144 1106 84-85 1.2156442688559785 1106 86-87 1.3346259620271326 1106 88-89 1.5068715670812374 1106 90-91 1.5890888120337863 1106 92-93 1.329011008890273 1106 94-95 1.268859726281768 1106 96-97 1.3396564517426945 1106 98-99 1.4500864717283442 1106 100-101 1.5662797737124912 1107 1 0.07140568217057464 1107 2 0.009904375259907283 1107 3 -0.0074919968915097 1107 4 0.031028838916697055 1107 5 -0.044254397810227886 1107 6 -0.04197632031218035 1107 7 -0.0058236893811240975 1107 8 0.06563684785795232 1107 9 0.05085726087843767 1107 10-11 0.00769693316068043 1107 12-13 -0.009030569393985388 1107 14-15 0.11652844933719564 1107 16-17 -0.019938617343484566 1107 18-19 0.04164795785833775 1107 20-21 0.09955483908862561 1107 22-23 0.1483259296806807 1107 24-25 0.2462228075998283 1107 26-27 0.18526546206527428 1107 28-29 0.13239376038200135 1107 30-31 0.14735418245312815 1107 32-33 0.22380694058175976 1107 34-35 0.1926171541811854 1107 36-37 0.18533211021888718 1107 38-39 0.1586204091240333 1107 40-41 0.14699085320064142 1107 42-43 0.136589708822612 1107 44-45 0.06024715003135128 1107 46-47 0.1493303176998424 1107 48-49 0.17223173403990444 1107 50-51 0.2599213838568133 1107 52-53 0.29045320224108906 1107 54-55 0.3597454551379329 1107 56-57 0.37943985008983105 1107 58-59 0.4360917309292063 1107 60-61 0.6004004924591229 1107 62-63 0.8680453057544248 1107 64-65 1.0288552858940818 1107 66-67 0.6341335678395943 1107 68-69 0.8480391202821451 1107 70-71 0.9481047600806534 1107 72-73 1.254077343193572 1107 74-75 1.3405488308333915 1107 76-77 1.1838003233519885 1107 78-79 1.3197350835617208 1107 80-81 1.3720235958918217 1107 82-83 1.6308173506643477 1107 84-85 1.7810502223671811 1107 86-87 2.1970244817225435 1107 88-89 2.1862818026253414 1107 90-91 2.2789595114324968 1107 92-93 2.300984845217158 1107 94-95 2.0727637712850964 1107 96-97 2.1260642265710565 1107 98-99 2.3929200407995346 1107 100-101 1.9760131638812553 1108 1 0.014135833252399976 1108 2 0.0156507751555921 1108 3 0.0213551688598983 1108 4 -0.03974282144340435 1108 5 -0.05605320453761209 1108 6 0.03343773222349711 1108 7 0.013276041301885755 1108 8 0.020267955062529097 1108 9 0.035188029004380894 1108 10-11 0.0701088899790605 1108 12-13 -0.013582946551608188 1108 14-15 0.152344388040504 1108 16-17 0.07518442193417485 1108 18-19 0.028366330210310764 1108 20-21 0.01584738083695214 1108 22-23 -0.06892730175918871 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-0.30568755860404195 2316 54-55 -0.27896834379919255 2316 56-57 -0.20342260523712952 2316 58-59 -0.2560286474372333 2316 60-61 -0.056646945840192586 2316 62-63 -0.028816750778670297 2316 64-65 0.04724367573277988 2316 66-67 0.05011704897530578 2316 68-69 0.36654669401836415 2316 70-71 0.12914151414724984 2316 72-73 0.313303850457455 2316 74-75 0.49923985931033243 2316 76-77 0.5890374096511692 2316 78-79 0.3014008464868958 2316 80-81 -0.17200056891237026 2316 82-83 0.521090462298897 2316 84-85 0.8047865861513408 2316 86-87 0.6502551423719183 2316 88-89 0.6796173068509255 2316 90-91 0.8360328187342461 2316 92-93 1.0092218272752582 2316 94-95 0.9534880650995845 2316 96-97 1.1238072636971417 2316 98-99 1.202839296996384 2316 100-101 0.9544201975721638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 32.0 5 70.0 6 134.0 7 344.0 8 931.0 9 1677.0 10 2731.0 11 4352.0 12 6375.0 13 8736.0 14 11716.0 15 16003.0 16 19935.0 17 24912.0 18 25380.0 19 24479.0 20 25641.0 21 27746.0 22 30028.0 23 33651.0 24 37653.0 25 42236.0 26 48944.0 27 57345.0 28 66877.0 29 78192.0 30 90202.0 31 104567.0 32 121517.0 33 145230.0 34 174132.0 35 210195.0 36 241500.0 37 211428.0 38 71231.0 39 1640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.306474314757764 19.147642679270085 15.379680851442359 23.166202154529795 2 16.063455469814535 20.15442531916548 39.340947867732154 24.44117134328783 3 19.53322085234838 23.67787410938437 29.69701281673392 27.09189222153333 4 13.594562784712863 16.789241843059052 36.00838918329969 33.60780618892839 5 14.065253760105378 37.05764512411041 34.13188776702897 14.745213348755238 6 33.162327749353075 35.98699442821628 16.668140414641535 14.182537407789114 7 29.397918051481575 32.05732168157693 20.062131181864565 18.48262908507693 8 26.77166194643584 35.13615151356535 19.12849615122898 18.963690388769834 9 26.110724322456385 16.914832526159053 19.721562987544274 37.25288016384029 10-11 25.279656382954936 26.433296133901692 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22.398657637279992 36-37 23.04643132113155 25.916061278265225 28.316372294174297 22.72113510642893 38-39 22.4710880567779 26.3038963652831 28.29548376997305 22.929531807965944 40-41 22.93898412075605 26.12314279223212 28.39872896926462 22.539144117747213 42-43 22.655589913829314 26.18833139491006 28.173400495867057 22.982678195393575 44-45 22.59145574525284 26.43083107399041 28.345601967724644 22.63211121303211 46-47 23.07225946121752 26.69943641787404 27.59547609730812 22.632828023600318 48-49 22.795247620151116 26.76168783781861 27.50844610046286 22.934618441567416 50-51 22.970064621307753 26.88563512439449 27.558731397743237 22.58556885655452 52-53 23.292180191607564 26.54354772809045 27.524225367573074 22.640046712728914 54-55 22.742675472564912 26.801047072526696 27.67719024358513 22.77908721132326 56-57 22.81857349327056 26.948828421178877 27.446294591040022 22.786303494510545 58-59 22.790953423465727 26.535619980363577 27.465656590296028 23.207770005874664 60-61 22.369842644371403 26.86779769200903 27.728669784914107 23.03368987870546 62-63 22.605337406976012 26.774875341248883 27.816662702792804 22.8031245489823 64-65 22.34064718880033 26.843734944902153 27.610160120176523 23.205457746120995 66-67 22.236513686731154 26.851092623681655 27.584031312706024 23.328362376881167 68-69 22.895110765990836 26.428889412893497 27.690150994637534 22.985848826478133 70-71 22.633059114572216 26.57777425433434 27.287434723128495 23.50173190796495 72-73 22.56844615184858 26.797320519501906 27.271004047984277 23.36322928066524 74-75 22.529566433509572 27.049500653531 27.152078594471313 23.268854318488117 76-77 22.60510872194543 26.743647129850412 27.423502316325266 23.227741831878895 78-79 22.643832720457453 26.91937887112938 27.380407892784326 23.056380515628845 80-81 22.777038361718272 26.885879389298236 27.13646774596002 23.200614503023477 82-83 22.7236599308958 27.009538290628953 27.17043246789612 23.096369310579124 84-85 22.532333399486244 27.115535761068905 27.18602545608027 23.166105383364584 86-87 22.794224520226173 27.053973729708524 26.876964859444897 23.27483689062041 88-89 22.5917687618281 27.252979511854015 26.578434899978248 23.576816826339638 90-91 22.464354565636818 27.15972086880005 26.875106244522613 23.50081832104052 92-93 22.525770366771624 26.865162692274076 26.97170494022657 23.63736200072773 94-95 22.21960222515673 26.68899818906819 27.097302710197805 23.99409687557727 96-97 22.524357496701857 26.378716639935927 27.090054132287683 24.006871731074536 98-99 22.212481933882582 26.65695009381168 26.72095649292703 24.40961147937871 100-101 22.345726380623752 26.307444467595296 27.115846872347888 24.230982279433064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 226.0 1 179.0 2 187.5 3 233.0 4 189.0 5 178.5 6 331.5 7 820.0 8 1338.0 9 1490.5 10 1339.5 11 1137.5 12 1115.5 13 1228.0 14 1374.5 15 1528.0 16 1721.5 17 1924.5 18 2126.5 19 2453.0 20 2836.5 21 3348.0 22 4128.5 23 4941.0 24 6046.5 25 7623.5 26 9678.0 27 12378.0 28 14882.5 29 17570.5 30 20983.5 31 25359.0 32 31665.5 33 34642.5 34 35099.0 35 37813.5 36 41690.5 37 45838.0 38 49505.5 39 52465.5 40 55597.0 41 59797.0 42 62325.5 43 64200.5 44 66569.5 45 69932.5 46 72459.0 47 73783.0 48 74947.5 49 74179.5 50 72580.0 51 70621.0 52 69084.0 53 69171.0 54 70002.5 55 66518.5 56 61205.5 57 56423.5 58 52310.5 59 47002.5 60 38611.5 61 32214.5 62 27058.5 63 20671.5 64 14796.0 65 10987.5 66 8256.5 67 6253.0 68 4922.5 69 3942.0 70 3101.5 71 2243.5 72 1674.5 73 1344.5 74 1070.5 75 812.5 76 563.0 77 398.0 78 250.5 79 153.5 80 99.5 81 60.0 82 36.0 83 20.0 84 10.0 85 3.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024138975450407874 2 0.011129338155030156 3 0.0016262046619222145 4 0.0 5 4.0655116548055364E-4 6 0.0 7 0.0 8 5.0818895685069204E-5 9 1.0163779137013841E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001346700735654334 26-27 0.008512165027249092 28-29 2.0327558274027682E-4 30-31 0.0 32-33 4.319606133230883E-4 34-35 0.009985913002116098 36-37 0.0013721101834968685 38-39 0.012501448338527024 40-41 0.011942440485991263 42-43 0.0054630312861449395 44-45 8.639212266461766E-4 46-47 1.0163779137013841E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.319606133230883E-4 68-69 0.001016377913701384 70-71 0.0 72-73 1.27047239212673E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5245668705520763E-4 82-83 9.65559018016315E-4 84-85 0.005513850181830009 86-87 0.0037605982806951208 88-89 0.0 90-91 0.0011942440485991264 92-93 4.0655116548055364E-4 94-95 2.5409447842534602E-5 96-97 0.0 98-99 0.0 100-101 1.0163779137013841E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1967772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.87512179440204 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36859346250252 45.35591891498312 2 12.713302072075125 14.715678116607434 3 3.8722825596899018 6.723264742931759 4 1.6801890346038622 3.889645800612692 5 0.8886114801695344 2.571424882135784 6 0.5464996505312173 1.897724030105541 7 0.3520495374776017 1.4262436901425375 8 0.24427282854293705 1.1309855762388454 9 0.1910489646064969 0.9951283885765873 >10 1.0326323187126567 10.92277254398791 >50 0.0693599557039994 2.7174354868073443 >100 0.03561928809769049 3.785008050947522 >500 0.0029892191705872154 1.213706751872069 >1k 0.0024617099051894684 2.3892514250159236 >5k 8.791821089962386E-5 0.2658115990349089 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5224 0.26547791105880153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4546 0.23102269978432463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3163 0.16074016705187388 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2483 0.12618331798602683 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2407 0.12232108191396157 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2346 0.11922112927717236 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2276 0.1156638065792175 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2110 0.10722786989549601 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.0163779137013841E-4 0.0 0.0 5.0818895685069204E-5 0.0 8 1.0163779137013841E-4 0.0 0.0 1.5245668705520763E-4 0.0 9 1.0163779137013841E-4 0.0 0.0 1.5245668705520763E-4 0.0 10-11 1.0163779137013841E-4 0.0 0.0 1.5245668705520763E-4 0.0 12-13 1.5245668705520763E-4 0.0 0.0 3.0491337411041525E-4 5.0818895685069204E-5 14-15 2.54094478425346E-4 5.0818895685069204E-5 0.0 3.0491337411041525E-4 5.0818895685069204E-5 16-17 2.54094478425346E-4 5.0818895685069204E-5 0.0 3.5573226979548444E-4 5.0818895685069204E-5 18-19 3.0491337411041525E-4 5.0818895685069204E-5 0.0 3.5573226979548444E-4 5.0818895685069204E-5 20-21 3.0491337411041525E-4 5.0818895685069204E-5 0.0 3.5573226979548444E-4 5.0818895685069204E-5 22-23 3.0491337411041525E-4 5.0818895685069204E-5 0.0 4.3196061332308823E-4 5.0818895685069204E-5 24-25 3.5573226979548444E-4 5.0818895685069204E-5 0.0 6.606456439058997E-4 5.0818895685069204E-5 26-27 3.5573226979548444E-4 5.0818895685069204E-5 0.0 0.0011180157050715224 1.0163779137013841E-4 28-29 5.335984046932266E-4 5.0818895685069204E-5 0.0 0.002820448710521341 1.0163779137013841E-4 30-31 5.590078525357612E-4 5.0818895685069204E-5 0.0 0.00861380281861923 1.0163779137013841E-4 32-33 5.590078525357612E-4 5.0818895685069204E-5 0.0 0.021470983426941738 1.0163779137013841E-4 34-35 6.352361960633652E-4 7.622834352760381E-5 0.0 0.0408329826829531 1.0163779137013841E-4 36-37 8.131023309611073E-4 1.5245668705520763E-4 0.0 0.0767365324844545 1.0163779137013841E-4 38-39 8.639212266461765E-4 2.286850305828114E-4 0.0 0.1293849084141862 1.0163779137013841E-4 40-41 8.639212266461765E-4 2.54094478425346E-4 0.0 0.19156182728486837 1.0163779137013841E-4 42-43 9.147401223312457E-4 2.54094478425346E-4 0.0 0.2504609273838636 1.0163779137013841E-4 44-45 0.0011180157050715224 2.54094478425346E-4 0.0 0.32557125520639585 1.2704723921267303E-4 46-47 0.0011942440485991261 2.54094478425346E-4 0.0 0.42080586572021556 1.5245668705520763E-4 48-49 0.00127047239212673 2.54094478425346E-4 0.0 0.5240444523044336 1.5245668705520763E-4 50-51 0.00127047239212673 2.54094478425346E-4 0.0 0.6354140621982628 1.5245668705520763E-4 52-53 0.00127047239212673 2.54094478425346E-4 0.0 0.7405329479228284 2.54094478425346E-4 54-55 0.00127047239212673 2.54094478425346E-4 0.0 0.8562221639498885 2.54094478425346E-4 56-57 0.00127047239212673 3.0491337411041525E-4 0.0 0.9965331349363646 2.54094478425346E-4 58-59 0.00127047239212673 3.0491337411041525E-4 0.0 1.1464742866551614 2.54094478425346E-4 60-61 0.00127047239212673 3.3032282195294985E-4 0.0 1.3112799653618405 2.54094478425346E-4 62-63 0.00127047239212673 3.5573226979548444E-4 0.0 1.4727061875054632 2.54094478425346E-4 64-65 0.00127047239212673 3.5573226979548444E-4 0.0 1.6419839290324285 2.54094478425346E-4 66-67 0.00127047239212673 3.8114171763801904E-4 0.0 1.8493504328753536 2.54094478425346E-4 68-69 0.0013721101834968685 4.0655116548055364E-4 0.0 2.0776543217405266 2.54094478425346E-4 70-71 0.0013721101834968685 4.573700611656228E-4 0.0 2.3202891391888896 2.54094478425346E-4 72-73 0.0013975196313394031 5.08188956850692E-4 0.0 2.570470562646485 2.54094478425346E-4 74-75 0.0014229290791819378 5.08188956850692E-4 0.0 2.8241076710106663 2.54094478425346E-4 76-77 0.0014229290791819378 5.08188956850692E-4 0.0 3.103662416174232 2.54094478425346E-4 78-79 0.0014229290791819378 5.335984046932266E-4 0.0 3.4176977820601167 2.54094478425346E-4 80-81 0.0015245668705520762 5.590078525357612E-4 0.0 3.7500787692883115 2.54094478425346E-4 82-83 0.0015499763183946108 5.590078525357612E-4 0.0 4.0942243308676005 3.0491337411041525E-4 84-85 0.0016770235576072838 5.590078525357612E-4 0.0 4.44398538042009 3.0491337411041525E-4 86-87 0.001727842453292353 6.098267482208305E-4 0.0 4.803122516226473 3.5573226979548444E-4 88-89 0.001727842453292353 6.098267482208305E-4 0.0 5.187287958157754 4.0655116548055364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3180 0.0 49.15273 1 GTATCAA 5540 0.0 42.10664 1 ATCAACG 7295 0.0 31.577223 3 TCAACGC 7425 0.0 31.024355 4 CAACGCA 7555 0.0 30.427647 5 TATCAAC 7615 0.0 30.37656 2 AACGCAG 7985 0.0 28.729609 6 TAAGGTG 1445 0.0 27.28147 5 TGGTATC 1585 0.0 25.172686 2 AAGGTAA 1260 0.0 24.88584 1 ACGCAGA 9200 0.0 24.832172 7 AGGTAAG 1305 0.0 24.386185 2 CGCAGAG 9380 0.0 24.305014 8 TACATGG 6350 0.0 23.487427 2 GTAAGGT 1395 0.0 23.152195 4 GTACATG 6670 0.0 22.93554 1 GTATAGG 635 0.0 22.445353 1 ACATGGG 6645 0.0 22.229153 3 TAAGGTA 795 0.0 22.10508 4 GGTAAGG 1500 0.0 21.848183 3 >>END_MODULE