FastQCFastQC Report
Thu 26 May 2016
SRR938347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645293
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69821.081989111922801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.8833196702893105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.6662089934339904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40030.6203383579242298No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14470.22423922156291792No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.20595295470429714No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.1955700743693175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.19293561219477046No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10330.16008231919453642No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9920.15372861630298174No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9760.15124912249164332No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8190.12691908946788513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.12350978547729481No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT7970.12350978547729481No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7740.11994551312349583No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC7470.11576136731686226No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7430.11514149386402765No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7410.11483155713761035No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7320.1134368418687325No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7280.11281696841589789No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT7180.11126728478381138No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.11080237969418544No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7090.10987256951493353No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6930.10739307570359512No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT6860.10630829716113456No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6740.10444867680263073No Hit
GTGTAGGGTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGA6700.10382880334979613No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA6630.10274402480733558No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG6510.10088440444883176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT802.2374661E-741.6168141
TACAATC702.0496905E-433.9360247
GGATATG3900.031.7080461
CGACGAT300.00394838431.67116294-95
GGTATCA41600.031.0982761
GTATCAA61850.030.144431
AATGGCC953.4593635E-530.036875
AGGGTCT1901.2732926E-1130.036875
CCTGACT650.0058359329.2371888
CTATACA1153.8243543E-628.9485784
CTAATGG1004.908505E-528.5350283
CCTAATG856.3582713E-427.9755152
TGGGAAC2053.092282E-1127.8152966
TACCTGG1205.32575E-627.7423862
ATACTCG1056.883631E-527.1530286
TGGGGAC1405.7804937E-727.1530286
GATTGGC4200.027.1488179
CATGGGG10550.027.0474194
GTACATG34500.026.7450491
ATGGGAC3050.026.5079465
Produced by FastQC (version 0.11.5)