##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938347_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645293 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23991737086874 31.0 31.0 34.0 28.0 34.0 2 31.30686835282577 31.0 31.0 34.0 28.0 34.0 3 31.29066176140141 31.0 31.0 34.0 28.0 34.0 4 34.96923568053582 37.0 35.0 37.0 32.0 37.0 5 34.98055456978458 37.0 35.0 37.0 32.0 37.0 6 35.030748822627864 37.0 35.0 37.0 32.0 37.0 7 34.98050343022472 37.0 35.0 37.0 32.0 37.0 8 34.97631308568356 37.0 35.0 37.0 32.0 37.0 9 36.462668896144855 39.0 37.0 39.0 32.0 39.0 10-11 36.34534932193593 39.0 37.0 39.0 32.0 39.0 12-13 36.267206524787966 39.0 37.0 39.0 32.0 39.0 14-15 37.19794496453549 39.5 36.5 41.0 31.5 41.0 16-17 37.06661005155797 39.0 36.0 41.0 31.5 41.0 18-19 37.30319250325046 39.5 37.0 41.0 32.0 41.0 20-21 37.3616868616272 40.0 37.0 41.0 32.0 41.0 22-23 37.272899287610436 40.0 37.0 41.0 32.0 41.0 24-25 37.149728108006755 40.0 36.5 41.0 31.0 41.0 26-27 36.96613088937893 39.0 36.0 41.0 31.0 41.0 28-29 36.7926941714849 39.0 36.0 41.0 31.0 41.0 30-31 36.497114489077056 39.0 35.5 40.5 30.0 41.0 32-33 36.1852383335942 38.5 35.0 40.0 30.0 41.0 34-35 35.9409617026684 38.0 35.0 40.0 30.0 41.0 36-37 35.666690170201754 38.0 35.0 40.0 29.0 41.0 38-39 35.472830946562254 38.0 35.0 40.0 27.0 41.0 40-41 35.37987937262608 38.0 35.0 40.0 27.0 41.0 42-43 35.045886132346084 38.0 35.0 40.0 25.5 41.0 44-45 34.616165059903025 38.0 34.0 40.0 24.0 41.0 46-47 34.12692838756968 38.0 33.5 40.0 21.5 41.0 48-49 33.701148935444834 38.0 33.0 40.0 18.0 41.0 50-51 33.01978791029811 37.5 32.5 39.5 15.0 40.5 52-53 33.09562865241061 38.0 33.0 39.5 13.5 40.5 54-55 33.27743986065245 38.0 33.0 40.0 9.0 41.0 56-57 33.05415989325779 38.0 33.0 40.0 8.0 41.0 58-59 32.78203079841251 38.0 32.5 40.0 4.0 41.0 60-61 32.415767721019755 37.5 32.0 40.0 2.0 41.0 62-63 31.957602980351563 37.0 31.0 39.5 2.0 41.0 64-65 31.55141462870355 36.0 31.0 39.0 2.0 40.5 66-67 31.08668697785347 36.0 30.0 39.0 2.0 40.0 68-69 30.565624452767967 35.0 29.5 38.5 2.0 40.0 70-71 30.04868873519471 35.0 29.0 38.0 2.0 40.0 72-73 29.446219779232067 34.5 28.0 37.5 2.0 39.5 74-75 28.801787715037975 34.0 27.5 37.0 2.0 39.0 76-77 28.119591410413562 34.0 26.0 36.0 2.0 39.0 78-79 27.378464511469986 33.5 25.5 36.0 2.0 37.5 80-81 26.685035325038392 33.0 24.5 35.0 2.0 37.0 82-83 25.987940361975106 32.0 21.0 35.0 2.0 36.5 84-85 25.304116114695184 32.0 19.0 35.0 2.0 36.0 86-87 24.62608226030656 31.0 14.5 34.5 2.0 35.5 88-89 23.862000672562694 31.0 4.5 34.0 2.0 35.0 90-91 23.035371528902374 30.5 2.0 34.0 2.0 35.0 92-93 22.174860102310113 29.5 2.0 34.0 2.0 35.0 94-95 21.247790538561553 29.0 2.0 34.0 2.0 35.0 96-97 20.071368355150295 28.0 2.0 34.0 2.0 35.0 98-99 18.667073716900696 25.5 2.0 33.5 2.0 35.0 100-101 14.889989508641811 10.0 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6096952402791764 1101 2 0.6594284772893069 1101 3 0.48525019065557373 1101 4 0.5518541578061615 1101 5 0.48180655980206666 1101 6 0.7323938636282605 1101 7 0.6772802305143912 1101 8 0.849641485992862 1101 9 0.958706230302397 1101 10-11 0.9183182820219997 1101 12-13 0.8542403745032345 1101 14-15 0.8537644481369284 1101 16-17 0.6488965885679292 1101 18-19 0.9513931908327322 1101 20-21 1.0562591348144608 1101 22-23 1.0387315676085507 1101 24-25 0.8387767873111045 1101 26-27 1.0863444084029226 1101 28-29 1.150896147948437 1101 30-31 0.9261614812626675 1101 32-33 1.027414237051019 1101 34-35 0.9370081989302719 1101 36-37 0.7646995566899548 1101 38-39 0.942702391965689 1101 40-41 0.8081186985981503 1101 42-43 0.9823573700480281 1101 44-45 0.8619026946583404 1101 46-47 0.48672780459680354 1101 48-49 0.4561597400203041 1101 50-51 0.6101820099743733 1101 52-53 0.283701233936533 1101 54-55 0.469201443492814 1101 56-57 0.5018336884418204 1101 58-59 0.5206793631367219 1101 60-61 0.7339076047189579 1101 62-63 0.7913358896778568 1101 64-65 0.6093674840356513 1101 66-67 0.8393417838848372 1101 68-69 0.775532673331309 1101 70-71 0.7841034893784702 1101 72-73 1.0194780295237962 1101 74-75 0.9608138221033151 1101 76-77 0.83995861849532 1101 78-79 1.1100289437876683 1101 80-81 1.0775576306008041 1101 82-83 1.1298372120166036 1101 84-85 1.1108471041013566 1101 86-87 0.9898267246223256 1101 88-89 1.099739605374456 1101 90-91 0.9116961704041486 1101 92-93 1.3552105643834658 1101 94-95 0.9549049049579281 1101 96-97 1.0730002242295669 1101 98-99 1.2398000557006021 1101 100-101 1.1777730256917653 1102 1 0.5809175600528569 1102 2 0.2871233363274506 1102 3 0.16798846009270108 1102 4 0.12087181462013064 1102 5 0.36023305477816336 1102 6 0.4169124471810832 1102 7 0.45515555975056543 1102 8 0.47131254247515386 1102 9 0.6319576204106738 1102 10-11 0.592850035021705 1102 12-13 0.5301622357850633 1102 14-15 0.7184849632086241 1102 16-17 0.8323543000107136 1102 18-19 0.8020906837602553 1102 20-21 0.755739673298514 1102 22-23 0.9335223197303009 1102 24-25 0.6563921419106578 1102 26-27 0.6933217764645718 1102 28-29 0.5043860513724852 1102 30-31 0.7577754701418016 1102 32-33 0.5950539795151712 1102 34-35 0.5527773921797063 1102 36-37 0.7775275734688094 1102 38-39 0.8180316281377671 1102 40-41 0.5558384332581809 1102 42-43 0.7346986050494877 1102 44-45 0.9131781799768532 1102 46-47 0.6662224880423153 1102 48-49 0.5926342303139336 1102 50-51 0.8622671723975515 1102 52-53 0.6792480098608351 1102 54-55 0.8043882545356311 1102 56-57 0.8277043352079829 1102 58-59 0.9761105405828232 1102 60-61 0.9032569364914664 1102 62-63 0.9920845971210319 1102 64-65 1.1432788097599769 1102 66-67 1.2466191226810572 1102 68-69 1.4506375411490815 1102 70-71 1.3913857057476058 1102 72-73 1.385039925924147 1102 74-75 1.0442447068950393 1102 76-77 1.2067764899224969 1102 78-79 1.4225472346861174 1102 80-81 1.7159831500623177 1102 82-83 1.7242304419453909 1102 84-85 1.7555037229678057 1102 86-87 1.5746233298315708 1102 88-89 1.4279149053451903 1102 90-91 1.6681345666581713 1102 92-93 1.4890999410296466 1102 94-95 1.806004797651326 1102 96-97 1.671917403041391 1102 98-99 1.6585274969258457 1102 100-101 1.3634622267453214 1103 1 0.17540734958908644 1103 2 -0.027337057080309535 1103 3 0.05286665379442468 1103 4 0.00847853194820658 1103 5 0.0037502702768037466 1103 6 0.16323011300339374 1103 7 0.2883794394140722 1103 8 0.010567319346030501 1103 9 0.3054573233221447 1103 10-11 0.1166210266719574 1103 12-13 0.21060051955151948 1103 14-15 0.30494554425125386 1103 16-17 0.3005228273897629 1103 18-19 0.2419380740720385 1103 20-21 0.2166295316341902 1103 22-23 0.5104995220944986 1103 24-25 0.2441700760508212 1103 26-27 0.39110846689831646 1103 28-29 0.6746529619644761 1103 30-31 0.5452907154743869 1103 32-33 0.547969356192155 1103 34-35 0.7037843659420986 1103 36-37 0.9240860373647379 1103 38-39 0.5198485590568609 1103 40-41 0.4140920217749269 1103 42-43 0.5355007440869244 1103 44-45 0.7614480301816826 1103 46-47 0.6062418769781175 1103 48-49 0.5312150853034581 1103 50-51 0.6225206358243938 1103 52-53 0.5777453673365258 1103 54-55 0.38736977250167826 1103 56-57 0.31999566960458026 1103 58-59 0.3158899132569388 1103 60-61 0.14360155918902962 1103 62-63 0.3635664434480752 1103 64-65 0.6279904775211733 1103 66-67 0.392786680613316 1103 68-69 0.46682104804466107 1103 70-71 0.351755659151614 1103 72-73 0.6581229230783521 1103 74-75 0.38398107991314845 1103 76-77 0.3460406485365013 1103 78-79 0.4006135010650773 1103 80-81 0.4474307530269499 1103 82-83 0.11875228577064334 1103 84-85 0.4836481705394995 1103 86-87 0.5840128910787179 1103 88-89 0.9273559098171553 1103 90-91 0.6496134420998949 1103 92-93 0.9865355979080306 1103 94-95 0.6760559281513743 1103 96-97 0.26853372101993855 1103 98-99 0.05882693089401059 1103 100-101 0.35992843683382425 1104 1 0.2726681341563726 1104 2 0.09254640645640322 1104 3 0.3442382392836585 1104 4 0.14047965775738191 1104 5 0.34870682266369357 1104 6 0.42327174765803477 1104 7 0.21756502804238664 1104 8 0.41756762108317247 1104 9 0.5477852127088596 1104 10-11 0.6746597859491104 1104 12-13 0.751920759014169 1104 14-15 0.8350279768104656 1104 16-17 0.8437701276030154 1104 18-19 0.8713364000647914 1104 20-21 0.6527322541146177 1104 22-23 0.5683924840122287 1104 24-25 0.8859231435004844 1104 26-27 0.7746899093032908 1104 28-29 0.8217548908001504 1104 30-31 0.840777589908626 1104 32-33 0.6997837092448975 1104 34-35 0.605462430158866 1104 36-37 0.6605871035871616 1104 38-39 0.8290720803546847 1104 40-41 0.6944844321983012 1104 42-43 0.8596323623361926 1104 44-45 0.5464806000439779 1104 46-47 0.47191052902447694 1104 48-49 -0.005316661754605434 1104 50-51 0.38595998285506994 1104 52-53 0.6105763970715756 1104 54-55 0.5990358433008751 1104 56-57 0.44517474679604163 1104 58-59 0.6231914450166727 1104 60-61 0.39301139683416153 1104 62-63 0.7481126840314793 1104 64-65 0.9637389758083756 1104 66-67 0.558224846051484 1104 68-69 0.7124287441167532 1104 70-71 0.8592800707007946 1104 72-73 0.7755848826455747 1104 74-75 0.9121567365717773 1104 76-77 0.8787867354621568 1104 78-79 0.9411172753149799 1104 80-81 1.2888501346940124 1104 82-83 1.28864044017892 1104 84-85 1.418438716785154 1104 86-87 1.1787127133327182 1104 88-89 1.4796946262425585 1104 90-91 1.592573711685553 1104 92-93 1.6514387504272818 1104 94-95 1.6803423705183107 1104 96-97 1.9065049317465679 1104 98-99 1.7329622257723365 1104 100-101 1.8057869043641173 1105 1 0.4974616151429885 1105 2 0.6101623017860192 1105 3 0.4579898613678637 1105 4 0.41333795881133994 1105 5 0.39687389794544714 1105 6 0.4057422823310688 1105 7 0.4674767720537574 1105 8 0.6951637470713337 1105 9 0.540549438965563 1105 10-11 0.5279272052466126 1105 12-13 0.5748816804797343 1105 14-15 0.6655643054100295 1105 16-17 0.6024113018824195 1105 18-19 0.5674972036933923 1105 20-21 0.7863262570897618 1105 22-23 0.6596430896309045 1105 24-25 0.7229064199036372 1105 26-27 0.6614690404748202 1105 28-29 0.6790212193753717 1105 30-31 0.7247641798676554 1105 32-33 0.7790351523824768 1105 34-35 0.5859000337835667 1105 36-37 0.8235794807043888 1105 38-39 0.7549492231492181 1105 40-41 0.5668772285619141 1105 42-43 0.5338533358545234 1105 44-45 0.38964301297488646 1105 46-47 0.41339741722787693 1105 48-49 0.3425166232915444 1105 50-51 0.4665383091319839 1105 52-53 0.41994954826078157 1105 54-55 0.49501672644507266 1105 56-57 0.4847572533635045 1105 58-59 0.6341205797184415 1105 60-61 0.5538990207881724 1105 62-63 0.4829150027901896 1105 64-65 0.9693617952767966 1105 66-67 0.8625996649745424 1105 68-69 0.9091397781847839 1105 70-71 1.0202504726855182 1105 72-73 0.9613180890525896 1105 74-75 0.9611515921606326 1105 76-77 1.0684981566392118 1105 78-79 1.2225114521239853 1105 80-81 1.6369962720032838 1105 82-83 1.6228494352292806 1105 84-85 1.578261182255762 1105 86-87 1.6841054585687232 1105 88-89 1.88520228686237 1105 90-91 2.020342575946625 1105 92-93 1.8960502658252132 1105 94-95 2.413498867254667 1105 96-97 2.449530030532408 1105 98-99 2.642141335518968 1105 100-101 2.3090627231970924 1106 1 0.0763283839025597 1106 2 6.676616635061805E-4 1106 3 -0.061152558233974474 1106 4 -0.08589634440458838 1106 5 -0.004351216909071809 1106 6 -0.2190662934729133 1106 7 0.26227003392205717 1106 8 0.2832188772397899 1106 9 -0.053631265475480916 1106 10-11 0.08994864475656783 1106 12-13 -0.05911525673312923 1106 14-15 0.206146234965928 1106 16-17 0.2930698011167223 1106 18-19 -0.09636190809834488 1106 20-21 0.0765253382536244 1106 22-23 0.1810825995849683 1106 24-25 -0.07724671945317851 1106 26-27 -0.181563118790109 1106 28-29 -0.06523605474408356 1106 30-31 -0.47508268077553595 1106 32-33 0.001852916547868233 1106 34-35 -0.12849506575701497 1106 36-37 -0.5477834595712565 1106 38-39 -0.40967558542657656 1106 40-41 0.05998595750525482 1106 42-43 -0.4378158831347534 1106 44-45 -0.43577812025635865 1106 46-47 -0.5965566409650762 1106 48-49 -0.10388460182331016 1106 50-51 -0.3014555652937432 1106 52-53 -0.3786721975278837 1106 54-55 -0.5065146671230778 1106 56-57 -1.212026820143386 1106 58-59 -0.7548533865909022 1106 60-61 -0.3327778551689491 1106 62-63 -0.3738996985880618 1106 64-65 -0.48622779124694304 1106 66-67 -1.1473543932184178 1106 68-69 -0.9292982003757082 1106 70-71 -0.8013421766253579 1106 72-73 -0.344194927792735 1106 74-75 -0.03578562070307001 1106 76-77 -0.2255294084450128 1106 78-79 -0.18637063057126824 1106 80-81 0.11708815561737396 1106 82-83 -0.018038773040245815 1106 84-85 -0.16216760794332075 1106 86-87 0.08992475412768286 1106 88-89 0.23971989788840276 1106 90-91 0.22478361729425345 1106 92-93 0.4726199442325658 1106 94-95 0.6324881474996893 1106 96-97 0.760402924866252 1106 98-99 0.5716972313842028 1106 100-101 0.3404562913441076 1107 1 0.4197586602546295 1107 2 0.17970498740245588 1107 3 0.1944001572186238 1107 4 0.23405859597963996 1107 5 0.25107934898269946 1107 6 0.34514252798741296 1107 7 0.4840122033435037 1107 8 0.3326939734325691 1107 9 0.36543673337541094 1107 10-11 0.583621749911508 1107 12-13 0.536687322432229 1107 14-15 0.7540537451280898 1107 16-17 0.5945609305703741 1107 18-19 0.4942108602404929 1107 20-21 0.5965635213015261 1107 22-23 0.7584564292677598 1107 24-25 0.5810489322486561 1107 26-27 0.7290969186238456 1107 28-29 0.7362154892037509 1107 30-31 0.5431209968310569 1107 32-33 0.6798463159475219 1107 34-35 0.7050953848617638 1107 36-37 0.47870829194034314 1107 38-39 0.5627434961444422 1107 40-41 0.5613160305009259 1107 42-43 0.4741561310436353 1107 44-45 0.38646599337891985 1107 46-47 0.48310975501212994 1107 48-49 0.5534281983196507 1107 50-51 0.5731613035606031 1107 52-53 0.7982297729254881 1107 54-55 0.5128483002969233 1107 56-57 0.6079439544431722 1107 58-59 0.3621477964502162 1107 60-61 0.4855065768695255 1107 62-63 0.5518820020505082 1107 64-65 0.5513954045022516 1107 66-67 0.8108297404584448 1107 68-69 0.8223321569569997 1107 70-71 0.9061446866417846 1107 72-73 0.6745030031294235 1107 74-75 0.699434847944076 1107 76-77 0.7285106882793428 1107 78-79 0.5489471053187636 1107 80-81 0.6022605561503092 1107 82-83 0.7465089003499266 1107 84-85 0.6839160283133836 1107 86-87 0.6278160003020261 1107 88-89 0.6245882817366137 1107 90-91 0.7146802651936177 1107 92-93 1.030999517206748 1107 94-95 0.9262838546212322 1107 96-97 0.9341131725357954 1107 98-99 0.6515558998182662 1107 100-101 1.247852221049298 1108 1 0.41782914959934203 1108 2 0.438646222153551 1108 3 0.48249215845821425 1108 4 0.24947884701960987 1108 5 0.4106564400587658 1108 6 0.42258761158068836 1108 7 0.5685487475514606 1108 8 0.5374302095521912 1108 9 0.49767041905744236 1108 10-11 0.5103161792129285 1108 12-13 0.6139322164674894 1108 14-15 0.888382069575421 1108 16-17 0.9469748547154992 1108 18-19 0.8661189494820576 1108 20-21 0.9073063489733713 1108 22-23 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2316 100-101 -3.3390532655226703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5152.0 3 930.0 4 740.0 5 716.0 6 719.0 7 944.0 8 1044.0 9 1231.0 10 1741.0 11 2487.0 12 3347.0 13 4580.0 14 5736.0 15 7209.0 16 9170.0 17 11022.0 18 9031.0 19 6493.0 20 6701.0 21 7045.0 22 7656.0 23 8543.0 24 9520.0 25 10734.0 26 12448.0 27 14509.0 28 16759.0 29 19475.0 30 22837.0 31 26709.0 32 32170.0 33 38765.0 34 48210.0 35 62562.0 36 77682.0 37 95507.0 38 52366.0 39 2803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.45320529908066 25.569706963567267 16.584423876858985 19.392663860493084 2 14.360007074659375 24.20619529162801 40.96491527474812 20.468882358964493 3 16.203606731862774 30.632706352198625 33.49857335027625 19.66511356566235 4 10.774775948958567 21.249627615997614 42.343009110995254 25.63258732404856 5 9.927758046890522 41.17216992847209 36.92551038148222 11.974561643155175 6 23.844639652286677 43.47998740438892 19.249103028065566 13.426269915258837 7 22.837079522554642 37.25778949000155 24.812897225236398 15.09223376220741 8 23.5901967167372 36.05512409121208 22.296113720566122 18.0585654714846 9 25.169036746310024 18.28650527183837 24.854059517055582 31.690398464796026 10-11 21.314728989873462 30.015784438528293 30.098505440049365 18.570981131548876 12-13 22.998712998712996 29.75996650695446 29.511172101533546 17.730148392798995 14-15 21.338866407116523 30.58671612742708 28.733797815965357 19.34061964949104 16-17 17.397137953967288 33.33844911272232 30.341765067908096 18.922647865402293 18-19 17.08222873354728 31.32365936468071 34.46335829341271 17.1307536083593 20-21 17.809618988326122 30.677785677347625 34.492244164418906 17.02035116990734 22-23 18.356500123630283 30.22578222312824 34.15645005142864 17.26126760181283 24-25 17.84728881873697 31.711009931785355 33.974598011162115 16.46710323831556 26-27 17.374564372133772 31.112454983505263 34.59050011435838 16.9224805300026 28-29 16.78451955019246 30.546676641716118 35.42680161409738 17.24200219399404 30-31 17.63117738643906 30.412327169645124 34.73921147798254 17.21728396593327 32-33 17.39188410785734 31.011505641183078 34.55886514265956 17.03774510830002 34-35 17.841580473583825 30.831640485679284 34.39894862530433 16.927830415432567 36-37 18.17579217653954 30.20644036890711 33.7558068137416 17.861960640811745 38-39 17.04774291963991 31.543703754230236 33.88023421113417 17.528319114995682 40-41 16.918385444443064 30.84761686241923 34.95927333837701 17.274724354760693 42-43 17.190555658473933 30.590941822006673 34.11268813070341 18.105814388815986 44-45 17.299874618936105 30.533388713844456 33.91906489593682 18.247671771282622 46-47 18.200550994075456 30.976483944838595 32.92046233905122 17.902502722034725 48-49 17.568377045305724 30.904603415074526 32.922186360624735 18.604833178995005 50-51 16.948132006766635 30.911721424093475 32.95589364632 19.184252922819887 52-53 17.522899521086156 31.583127973837975 32.66804606252228 18.225926442553586 54-55 17.869309551707676 30.967957421218717 32.37224195758241 18.7904910694912 56-57 17.80351407987898 31.173276749290117 32.13185239376542 18.891356777065482 58-59 17.73829665045071 30.257081143585218 32.79906191849448 19.205560287469588 60-61 18.057808611379585 31.132292634684084 32.17738328955537 18.632515464380965 62-63 17.631500637005857 30.641376552604903 32.847571163318385 18.87955164707086 64-65 17.75431445332176 30.44969506288602 32.56278914780344 19.233201335988777 66-67 17.703957366116228 30.506598968250092 31.712709494284457 20.076734171349216 68-69 17.70388062107296 30.04614030247704 32.2603168600416 19.9896622164084 70-71 18.21528201399703 30.43923446533724 32.262227634248 19.083255886417735 72-73 18.271101417328207 29.650341736851 32.41865050760595 19.659906338214846 74-75 17.62187266985904 30.85916608663435 31.612794711965513 19.906166531541096 76-77 17.762841757063004 30.597700899984808 32.13020172928531 19.509255613666873 78-79 18.057912526791984 30.089546826108993 31.759677271874885 20.092863375224137 80-81 18.22678993604839 30.16496617220984 31.314359395183445 20.293884496558327 82-83 18.515794930527413 30.202656471206325 31.408459762775777 19.873088835490492 84-85 18.19657636840266 29.839961379340274 31.304276401180626 20.65918585107644 86-87 18.059571368235062 30.085672105611895 31.65458370951305 20.200172816639995 88-89 18.921099876282522 29.721263592643925 31.211919667358117 20.145716863715432 90-91 18.857691213551934 29.53245861332048 31.478990984342943 20.13085918878465 92-93 19.484809683110054 29.60964652555799 31.05806703643878 19.847476754893176 94-95 19.265669563707373 28.864277867833856 31.308738333732578 20.56131423472619 96-97 19.896340362064542 28.631362544389845 30.75757892884524 20.714718164700376 98-99 19.640897338120126 28.68341425001705 30.4162248801751 21.259463531687718 100-101 20.1111210195528 28.33872912603667 31.108534916271513 20.441614938139015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2540.0 1 1578.5 2 706.5 3 750.5 4 625.0 5 646.5 6 1039.0 7 1984.0 8 2928.5 9 3158.5 10 2525.5 11 1773.0 12 1548.5 13 1487.5 14 1481.5 15 1497.5 16 1532.5 17 1595.0 18 1716.0 19 1829.0 20 1954.5 21 2168.5 22 2414.0 23 2678.0 24 3026.0 25 3802.5 26 5408.0 27 7326.5 28 8126.0 29 9136.0 30 11764.5 31 14332.0 32 17438.0 33 22451.0 34 28426.5 35 32442.0 36 37234.0 37 42563.0 38 41896.5 39 38879.0 40 36047.5 41 32709.5 42 29715.0 43 27472.5 44 25669.5 45 23842.5 46 20772.0 47 17009.5 48 15397.0 49 13192.0 50 9314.5 51 5914.5 52 3806.5 53 2673.0 54 2065.5 55 1732.5 56 1480.0 57 1308.5 58 1230.0 59 1240.0 60 1297.0 61 1293.0 62 1126.0 63 837.5 64 553.5 65 397.5 66 312.5 67 275.0 68 234.5 69 186.0 70 164.5 71 147.0 72 120.5 73 105.5 74 91.0 75 89.5 76 76.0 77 53.5 78 49.5 79 47.5 80 38.5 81 26.0 82 13.0 83 9.5 84 7.5 85 3.5 86 1.5 87 1.0 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0075934497972238975 2 0.11452162041119306 3 0.12025544984991315 4 0.12350978547729481 5 0.16566118027004786 6 0.0967002586421982 7 0.029908894099269633 8 0.031458577731356144 9 0.025879716655844712 10-11 0.05485880057586244 12-13 0.05935288310891331 14-15 0.03138109354975182 16-17 0.035642723537989716 18-19 0.040756679523875204 20-21 0.06067011419618684 22-23 0.03479039754034214 24-25 0.053231632762171605 26-27 0.06051514583297819 28-29 0.05431641130463216 30-31 0.06834104817501507 32-33 0.05400647457821485 34-35 0.06555161763725935 36-37 0.055943579118323 38-39 0.03626259699082432 40-41 0.0404467427974579 42-43 0.03556523935638539 44-45 0.009298101792519057 46-47 0.014799478686426165 48-49 0.010150427790166637 50-51 0.010615332879792591 52-53 0.012862374146318028 54-55 0.013792184325569935 56-57 0.01813129849541216 58-59 0.022702865210067365 60-61 0.028126757922370148 62-63 0.013482247599152633 64-65 0.012087532330274774 66-67 0.0121650165118791 68-69 0.013482247599152633 70-71 0.035255302629968094 72-73 0.0319234828209821 74-75 0.033163229726651304 76-77 0.026499590108679315 78-79 0.006818607981180642 80-81 0.0061212503467417126 82-83 0.005966281983533062 84-85 0.006586155436367665 86-87 0.015574320502469421 88-89 0.04261629988237901 90-91 0.03850963825734976 92-93 0.03269832463702535 94-95 0.05028723386120723 96-97 0.04610308805457366 98-99 0.028669147193600426 100-101 0.0775616657859298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 645293.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.05301803796737 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17443554253951 17.019292190895737 2 8.200453312127767 3.6168948962372585 3 3.1293264882735863 2.070332804777595 4 1.7974547558297855 1.5855720861097797 5 1.1770630680178378 1.2978896535411295 6 0.8821215225159856 1.167206510863456 7 0.6650943540521921 1.0267136450804273 8 0.5706454827221297 1.006756409900458 9 0.42175014116165715 0.8370777123497979 >10 4.43730753684991 21.092983624572543 >50 0.8532153202299484 13.145958924244772 >100 0.6525413222153388 27.300336393274588 >500 0.03227623744290923 4.608675454638302 >1k 0.004911601350007926 2.2619398937651995 >5k 0.001403314671430836 1.9623697997489575 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6982 1.081989111922801 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5700 0.8833196702893105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.6662089934339904 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4003 0.6203383579242298 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1447 0.22423922156291792 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1329 0.20595295470429714 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1262 0.1955700743693175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.19293561219477046 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1033 0.16008231919453642 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 992 0.15372861630298174 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 976 0.15124912249164332 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 819 0.12691908946788513 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.12350978547729481 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 797 0.12350978547729481 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 774 0.11994551312349583 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 747 0.11576136731686226 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 743 0.11514149386402765 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 741 0.11483155713761035 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 732 0.1134368418687325 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 728 0.11281696841589789 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 718 0.11126728478381138 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 715 0.11080237969418544 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 709 0.10987256951493353 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 693 0.10739307570359512 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 686 0.10630829716113456 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 674 0.10444867680263073 No Hit GTGTAGGGTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGA 670 0.10382880334979613 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 663 0.10274402480733558 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 651 0.10088440444883176 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5496836320865096E-4 0.0 12-13 0.0 0.0 0.0 3.0993672641730193E-4 0.0 14-15 0.0 0.0 0.0 3.0993672641730193E-4 0.0 16-17 0.0 0.0 0.0 6.973576344389294E-4 0.0 18-19 0.0 0.0 0.0 0.0012397469056692077 0.0 20-21 0.0 0.0 0.0 0.002092072903316788 0.0 22-23 0.0 0.0 0.0 0.007128544707597944 0.0 24-25 0.0 0.0 0.0 0.014954447049634816 0.0 26-27 0.0 0.0 0.0 0.02603468501905336 0.0 28-29 0.0 0.0 0.0 0.052689243490941326 0.0 30-31 0.0 0.0 0.0 0.11537394640884063 0.0 32-33 0.0 0.0 0.0 0.2052555970698582 0.0 34-35 0.0 0.0 0.0 0.3367462532523985 0.0 36-37 0.0 0.0 0.0 0.542079334503861 0.0 38-39 0.0 0.0 0.0 0.7989393965222 0.0 40-41 0.0 0.0 0.0 1.063935297608993 0.0 42-43 0.0 0.0 0.0 1.3267616416108652 0.0 44-45 0.0 0.0 0.0 1.63406390585362 0.0 46-47 0.0 0.0 0.0 2.0166033104341747 0.0 48-49 0.0 0.0 0.0 2.4061937755407232 0.0 50-51 0.0 0.0 0.0 2.7888106643028827 0.0 52-53 0.0 0.0 0.0 3.14756242513091 0.0 54-55 0.0 0.0 0.0 3.5442039507634515 0.0 56-57 0.0 0.0 0.0 4.023056193078183 0.0 58-59 0.0 0.0 0.0 4.515933072263298 0.0 60-61 0.0 0.0 0.0 5.033372437016983 0.0 62-63 0.0 0.0 0.0 5.521600265305838 0.0 64-65 0.0 0.0 0.0 6.06298224217526 0.0 66-67 0.0 0.0 0.0 6.663639617971991 0.0 68-69 0.0 0.0 0.0 7.3114848603657565 0.0 70-71 7.748418160432548E-5 0.0 0.0 8.027826119297746 0.0 72-73 1.5496836320865096E-4 0.0 0.0 8.69302781837088 1.5496836320865096E-4 74-75 1.5496836320865096E-4 0.0 0.0 9.408981656394847 1.5496836320865096E-4 76-77 1.5496836320865096E-4 0.0 0.0 10.174292918100768 1.5496836320865096E-4 78-79 1.5496836320865096E-4 0.0 0.0 11.007480322892082 1.5496836320865096E-4 80-81 1.5496836320865096E-4 0.0 0.0 11.90149281024279 1.5496836320865096E-4 82-83 1.5496836320865096E-4 1.5496836320865096E-4 0.0 12.760017542418716 1.5496836320865096E-4 84-85 1.5496836320865096E-4 1.5496836320865096E-4 0.0 13.586463823410451 1.5496836320865096E-4 86-87 1.5496836320865096E-4 1.5496836320865096E-4 0.0 14.460562876088847 1.5496836320865096E-4 88-89 1.5496836320865096E-4 1.5496836320865096E-4 0.0 15.378905396463313 1.5496836320865096E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 80 2.2374661E-7 41.616814 1 TACAATC 70 2.0496905E-4 33.936024 7 GGATATG 390 0.0 31.708046 1 CGACGAT 30 0.003948384 31.671162 94-95 GGTATCA 4160 0.0 31.098276 1 GTATCAA 6185 0.0 30.14443 1 AATGGCC 95 3.4593635E-5 30.03687 5 AGGGTCT 190 1.2732926E-11 30.03687 5 CCTGACT 65 0.00583593 29.237188 8 CTATACA 115 3.8243543E-6 28.948578 4 CTAATGG 100 4.908505E-5 28.535028 3 CCTAATG 85 6.3582713E-4 27.975515 2 TGGGAAC 205 3.092282E-11 27.815296 6 TACCTGG 120 5.32575E-6 27.742386 2 ATACTCG 105 6.883631E-5 27.153028 6 TGGGGAC 140 5.7804937E-7 27.153028 6 GATTGGC 420 0.0 27.148817 9 CATGGGG 1055 0.0 27.047419 4 GTACATG 3450 0.0 26.745049 1 ATGGGAC 305 0.0 26.507946 5 >>END_MODULE