FastQCFastQC Report
Thu 26 May 2016
SRR938347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645293
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64360.9973763856108776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56350.8732467266807481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.6649692465283212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42180.6536565560140898No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19110.29614454209173197No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15040.23307241826581102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.19851447327028188No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT12470.19324554892118775TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.18999121329380605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.1779036809635313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10070.1560531417511115No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9770.15140409085485199No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC9730.1507842174020174No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT8790.13621719126040419No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC8490.13156814036414466No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8360.12955355164243218No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8120.12583431092542458No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8110.1256793425622159No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7900.12242500693483424No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7620.11808589276499204No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA7590.11762098767536606No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7560.11715608258574012No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7510.11638124076969686No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT7480.11591633568007091No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT7390.11452162041119306RNA PCR Primer, Index 11 (95% over 23bp)
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA7170.11111231642060274No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7060.10940766442530758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.10816791751963838No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6970.1080129491564297No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6850.10615332879792591No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG255.2527314E-576.060751
CGTCAGA353.3991473E-667.9113852
GTTGTAC405.3837255E-447.5048073
TAGACCC901.1941665E-842.2264945
TCGTATC250.001613856838.0097494-95
TTGGACG250.001617548237.99206516-17
CATGGCA550.002581432734.5382429
TGGGGCC550.002581432734.5382428
CCGGGTC702.0548567E-433.9214869
CTTTAGG2150.033.1660232
AGGGGTG1753.6379788E-1232.564638
TCTAGAC1351.1943484E-831.669873
CGTAATC300.003953671531.66250622-23
TACGTGT300.003953671531.66250636-37
TGGACGT300.003954421331.66128218-19
AGCTGAT600.003953718631.6600537
CCTTTAG2300.031.0030231
GGTATCA45400.030.2609541
ATGCCGC404.4872047E-429.69510794-95
GTCAGAT804.4888185E-429.6905043
Produced by FastQC (version 0.11.5)