##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645293 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775963786992886 31.0 31.0 34.0 30.0 34.0 2 32.011503301600975 31.0 31.0 34.0 30.0 34.0 3 32.032518561335706 33.0 31.0 34.0 30.0 34.0 4 35.733197167798195 37.0 35.0 37.0 33.0 37.0 5 35.64487294918742 37.0 35.0 37.0 33.0 37.0 6 35.656520371366184 37.0 35.0 37.0 33.0 37.0 7 35.60857625915669 37.0 35.0 37.0 33.0 37.0 8 35.61009339943251 37.0 35.0 37.0 33.0 37.0 9 37.136635605841064 39.0 37.0 39.0 33.0 39.0 10-11 37.06339135865413 39.0 37.0 39.0 33.0 39.0 12-13 36.99829379832107 39.0 37.0 39.0 33.0 39.0 14-15 37.95102379849154 40.0 37.0 41.0 32.5 41.0 16-17 37.87202557597867 40.0 37.0 41.0 32.5 41.0 18-19 38.18059315690702 40.0 37.5 41.0 33.0 41.0 20-21 38.20763823565419 40.0 37.0 41.0 33.0 41.0 22-23 38.091505719107445 40.0 37.0 41.0 33.0 41.0 24-25 37.950789796263095 40.0 37.0 41.0 33.0 41.0 26-27 37.754546384355635 40.0 37.0 41.0 32.0 41.0 28-29 37.637303519486494 39.5 36.5 41.0 32.0 41.0 30-31 37.33018876696322 39.0 36.0 41.0 32.0 41.0 32-33 37.05544458098879 39.0 35.0 41.0 31.5 41.0 34-35 36.84823483285887 39.0 35.0 41.0 31.0 41.0 36-37 36.72706119545695 39.0 35.0 41.0 31.0 41.0 38-39 36.53479194722398 39.0 35.0 41.0 30.0 41.0 40-41 36.377169750795375 39.0 35.0 41.0 30.0 41.0 42-43 36.109426260628894 39.0 35.0 40.5 30.0 41.0 44-45 35.704441238321195 39.0 35.0 40.0 28.5 41.0 46-47 35.20525559706986 38.0 35.0 40.0 26.0 41.0 48-49 34.760085728498524 38.0 34.0 40.0 24.0 41.0 50-51 34.41595445789742 38.0 34.0 40.0 23.5 41.0 52-53 34.0360099985588 38.0 33.0 40.0 22.5 41.0 54-55 33.73577971557107 38.0 33.0 40.0 20.5 41.0 56-57 33.43178680072463 38.0 33.0 40.0 19.0 41.0 58-59 33.00020223371399 37.5 32.5 40.0 14.5 41.0 60-61 32.63182616268889 37.0 32.0 40.0 10.0 41.0 62-63 32.1844464452582 36.5 31.0 39.0 7.5 40.5 64-65 31.706229573232626 36.0 31.0 39.0 4.0 40.0 66-67 31.216862727474187 35.5 30.0 39.0 2.0 40.0 68-69 30.72205106827441 35.0 29.0 38.0 2.0 40.0 70-71 30.229389595114156 35.0 29.0 38.0 2.0 40.0 72-73 29.759054414041373 34.5 28.5 37.0 2.0 39.0 74-75 29.167376680050765 34.0 28.0 36.5 2.0 39.0 76-77 27.846029633050414 32.5 26.0 35.0 2.0 37.5 78-79 28.513049111023985 34.0 28.0 36.0 2.0 38.0 80-81 28.124090141997513 34.0 26.5 35.0 2.0 37.0 82-83 27.698317663449007 33.0 26.0 35.0 2.0 37.0 84-85 27.164189755661383 33.0 26.0 35.0 2.0 36.0 86-87 26.65111894906655 32.5 25.0 35.0 2.0 36.0 88-89 25.9847960538856 32.0 23.5 34.5 2.0 35.0 90-91 25.451101282673143 32.0 21.0 34.0 2.0 35.0 92-93 24.754330978330774 31.0 18.5 34.0 2.0 35.0 94-95 24.031691805117987 31.0 10.0 34.0 2.0 35.0 96-97 23.080634688428358 31.0 2.0 34.0 2.0 35.0 98-99 21.918948446674612 30.0 2.0 34.0 2.0 35.0 100-101 18.00146057682324 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30297305276321396 1101 2 0.2837120326042566 1101 3 0.4519289694680211 1101 4 0.3503259145205888 1101 5 0.16642616660146814 1101 6 0.3220453747183285 1101 7 0.49379708786223375 1101 8 0.26005821877403434 1101 9 0.2683846288062881 1101 10-11 0.37800404274091903 1101 12-13 0.3061403558233735 1101 14-15 0.44911652908067623 1101 16-17 0.573655706131639 1101 18-19 0.6326764261064142 1101 20-21 0.5616117020680349 1101 22-23 0.46220937275930396 1101 24-25 0.4468288279771073 1101 26-27 0.16171435058891603 1101 28-29 0.4171175994001928 1101 30-31 0.7560209425801503 1101 32-33 0.5596918989162916 1101 34-35 0.49240293864142615 1101 36-37 0.4161257415936035 1101 38-39 0.5994345963868426 1101 40-41 0.8058207307917016 1101 42-43 0.5275861329807867 1101 44-45 0.6999469179232634 1101 46-47 0.5345047448721445 1101 48-49 0.5670165109078837 1101 50-51 0.7698012166052308 1101 52-53 0.8937442397007729 1101 54-55 0.7541969935861275 1101 56-57 0.46058082731566685 1101 58-59 0.6016329969623868 1101 60-61 0.8476482133843817 1101 62-63 1.2645708478814583 1101 64-65 1.3531006923407887 1101 66-67 1.0019271540810344 1101 68-69 1.131178303605278 1101 70-71 1.3949733685736199 1101 72-73 1.4262907071093878 1101 74-75 1.458180231590358 1101 76-77 1.2612335100661554 1101 78-79 1.54377013164056 1101 80-81 1.4920871606976078 1101 82-83 1.5422683089541636 1101 84-85 1.7073699875497006 1101 86-87 1.5129995076928893 1101 88-89 1.8552582489517668 1101 90-91 1.7038552000496665 1101 92-93 1.649317618344405 1101 94-95 1.177024784776723 1101 96-97 1.6435810372690547 1101 98-99 1.68672203031511 1101 100-101 2.344854760919361 1102 1 0.3099235941738101 1102 2 0.2483008532071267 1102 3 0.39561739010991204 1102 4 0.06798760606287857 1102 5 -0.002191529233087408 1102 6 0.026513231512787172 1102 7 -0.005959033491777177 1102 8 0.2748372647207731 1102 9 0.35218133156699594 1102 10-11 0.42526772433296145 1102 12-13 0.397094896843754 1102 14-15 0.49277080671212303 1102 16-17 0.4638493457765449 1102 18-19 0.35833733723703176 1102 20-21 0.38737260344350943 1102 22-23 0.2935916769247484 1102 24-25 0.28789311438819 1102 26-27 0.4730254421897371 1102 28-29 0.4762825589163313 1102 30-31 0.6014262688897816 1102 32-33 0.6735344508624408 1102 34-35 0.6361328186531381 1102 36-37 0.7887967005244434 1102 38-39 0.7661134569858135 1102 40-41 0.8467923433173112 1102 42-43 0.9158043555932167 1102 44-45 1.1182597654503326 1102 46-47 0.7764933313515101 1102 48-49 0.9319321288963707 1102 50-51 0.9892798040601036 1102 52-53 0.9077916497095515 1102 54-55 0.7174688695006779 1102 56-57 1.0003940162728355 1102 58-59 0.9993380989036567 1102 60-61 0.9109713038145841 1102 62-63 1.2531207454524278 1102 64-65 1.0492766751717326 1102 66-67 1.0165598728401797 1102 68-69 1.1322269817830168 1102 70-71 0.9272385134375334 1102 72-73 0.936582385969988 1102 74-75 0.9254639112313683 1102 76-77 0.7603311590760065 1102 78-79 1.0521961388593688 1102 80-81 1.1934211768911496 1102 82-83 0.99502901522672 1102 84-85 1.071200345563554 1102 86-87 0.837467852244643 1102 88-89 0.8237247400258134 1102 90-91 1.0528943181844852 1102 92-93 1.2649770662031479 1102 94-95 1.199205547629127 1102 96-97 1.016861692814853 1102 98-99 1.28420032511562 1102 100-101 1.3679012929650156 1103 1 -0.00642096597879771 1103 2 0.07458224295601212 1103 3 -0.01681185591066736 1103 4 -0.08206555714818364 1103 5 -0.05396311506039808 1103 6 -0.22124296825580103 1103 7 -0.024972699564266065 1103 8 0.026862157612534077 1103 9 0.08830416985701817 1103 10-11 0.0010253000905393606 1103 12-13 0.05245265398170318 1103 14-15 0.19219539313362333 1103 16-17 0.2551485608373767 1103 18-19 0.2559277928770243 1103 20-21 0.11898829597251392 1103 22-23 0.009496858774205919 1103 24-25 0.12458042088973542 1103 26-27 0.18307485270354107 1103 28-29 0.26622533028325535 1103 30-31 0.31508765050781307 1103 32-33 0.07318107187129641 1103 34-35 0.5089906539722691 1103 36-37 0.5998562120483442 1103 38-39 0.6233897805933992 1103 40-41 0.7242667780672889 1103 42-43 0.9242987421835807 1103 44-45 0.6056139575258825 1103 46-47 0.9740454782070671 1103 48-49 1.0296038929768088 1103 50-51 1.0282101289811436 1103 52-53 1.1514667912175085 1103 54-55 1.003096039028776 1103 56-57 0.7250281282908588 1103 58-59 0.7380839162098454 1103 60-61 0.993065465243113 1103 62-63 0.9556608524043 1103 64-65 1.1414992101187238 1103 66-67 0.9031189621856477 1103 68-69 0.9255080900795996 1103 70-71 0.619306249622916 1103 72-73 0.9126745772324547 1103 74-75 1.1372450351641952 1103 76-77 0.9417662530498028 1103 78-79 1.1342824821686186 1103 80-81 1.0416519364996155 1103 82-83 1.2018729968385031 1103 84-85 1.075957671141314 1103 86-87 0.8801430562975554 1103 88-89 1.062654283134922 1103 90-91 1.3438847175340172 1103 92-93 1.7036869201606812 1103 94-95 1.5077515338066938 1103 96-97 1.541019681369125 1103 98-99 1.284145598280709 1103 100-101 1.5986218108372334 1104 1 0.31067434492456414 1104 2 0.35252272213487856 1104 3 0.277219580535629 1104 4 0.20201869597632083 1104 5 0.11430732255988119 1104 6 0.07464960317857106 1104 7 0.2593651141853073 1104 8 0.21716194233956543 1104 9 0.1893125804629534 1104 10-11 0.3060308403901999 1104 12-13 0.3288869830476102 1104 14-15 0.42061041102231655 1104 16-17 0.3755036471367319 1104 18-19 0.32287540471627807 1104 20-21 0.4239165002815284 1104 22-23 0.3954288081736408 1104 24-25 0.3595677301804514 1104 26-27 0.28732503590109104 1104 28-29 0.43256236813732585 1104 30-31 0.5768281413504752 1104 32-33 0.40017285397143354 1104 34-35 0.3353688193597222 1104 36-37 0.15679705381891296 1104 38-39 0.3851074509798025 1104 40-41 0.498885613057638 1104 42-43 0.3708697150703415 1104 44-45 0.37238889457946556 1104 46-47 0.5330071981594884 1104 48-49 0.511114252196144 1104 50-51 0.23267761363439377 1104 52-53 0.21286142536756358 1104 54-55 0.2972250963157279 1104 56-57 0.42322125527654464 1104 58-59 0.30244267406705916 1104 60-61 0.642931204892129 1104 62-63 0.4091223352775444 1104 64-65 0.21991790463649608 1104 66-67 0.22782996646321152 1104 68-69 0.21670852214690584 1104 70-71 0.012426642743310623 1104 72-73 0.3699863781975061 1104 74-75 0.6736753579722254 1104 76-77 0.5338252413936182 1104 78-79 0.3991975520372506 1104 80-81 0.3558262290020835 1104 82-83 0.5820719405049424 1104 84-85 0.5473750496794345 1104 86-87 0.5303003906654169 1104 88-89 0.5435180627603096 1104 90-91 0.5710889834379742 1104 92-93 0.8929800692061534 1104 94-95 1.1340006368359816 1104 96-97 1.3178685820570344 1104 98-99 1.3658555097119809 1104 100-101 1.3563750608505494 1105 1 0.15573578326588589 1105 2 0.23954288345465358 1105 3 0.11533983753707133 1105 4 0.24558672125770187 1105 5 0.1404432684327901 1105 6 0.24814992090012566 1105 7 0.13587003095686612 1105 8 0.13877845120046572 1105 9 0.4894462426360704 1105 10-11 0.31347599385822633 1105 12-13 0.3095790073843503 1105 14-15 0.39012740121312106 1105 16-17 0.2974817012200006 1105 18-19 0.38675569794528286 1105 20-21 0.3676834363014123 1105 22-23 0.3575298606719528 1105 24-25 0.26775228623456115 1105 26-27 0.24655685577609887 1105 28-29 0.30319004472842437 1105 30-31 0.4219054049035478 1105 32-33 0.2635513662554487 1105 34-35 0.2085224432114856 1105 36-37 4.7682944175875264E-4 1105 38-39 0.09439344177196318 1105 40-41 0.06662238865857262 1105 42-43 0.050042762543540675 1105 44-45 0.08864406516307355 1105 46-47 0.17868557629440573 1105 48-49 -0.12459180788609814 1105 50-51 0.08399974921019293 1105 52-53 -0.18310606721284728 1105 54-55 0.011661584871667685 1105 56-57 -0.16375410852072037 1105 58-59 -0.2507957729197017 1105 60-61 0.026682851322810563 1105 62-63 -7.640397020693968E-4 1105 64-65 0.17324448587947927 1105 66-67 -0.008578002626496328 1105 68-69 -0.13552417176671483 1105 70-71 0.06960556745669066 1105 72-73 0.13463261167537866 1105 74-75 0.34151877115389695 1105 76-77 0.16575759713463967 1105 78-79 0.4937166725450197 1105 80-81 0.5477823956001764 1105 82-83 0.6350839652312246 1105 84-85 0.8336604342442548 1105 86-87 0.8707043474552805 1105 88-89 0.912613128459288 1105 90-91 1.0243583504538485 1105 92-93 1.3987100131849033 1105 94-95 1.3995416034399142 1105 96-97 1.464899407134883 1105 98-99 1.2721118245801328 1105 100-101 1.205280808323991 1106 1 -0.13982317538648914 1106 2 0.0680268038221854 1106 3 0.06020966908377545 1106 4 0.05416252523932741 1106 5 0.00874342157214869 1106 6 -0.14541822611365518 1106 7 0.04704920400434531 1106 8 -0.08327361618084694 1106 9 -0.19508668232564474 1106 10-11 0.15727384990723436 1106 12-13 0.04081943617455153 1106 14-15 0.04403245481189799 1106 16-17 -0.011094102761624924 1106 18-19 -0.103290243861764 1106 20-21 -0.12925377656229387 1106 22-23 0.11174119298436125 1106 24-25 0.11461292941986301 1106 26-27 -0.13552583947658547 1106 28-29 0.020647931405299857 1106 30-31 -0.22051419693412555 1106 32-33 0.050687660283010416 1106 34-35 0.02322382146569879 1106 36-37 -0.06154461006819645 1106 38-39 0.03084060869385752 1106 40-41 -0.0528263096568935 1106 42-43 -0.03188679335232081 1106 44-45 -0.27506190727184077 1106 46-47 -0.8259086044100385 1106 48-49 -0.27466837756450246 1106 50-51 0.22948213818416008 1106 52-53 0.1438464595865412 1106 54-55 0.3945099769084166 1106 56-57 0.5353270553383851 1106 58-59 0.2583925884891798 1106 60-61 0.06956187123093116 1106 62-63 0.022206863820137812 1106 64-65 0.19544969261761125 1106 66-67 0.277026897832922 1106 68-69 -0.09417654542674825 1106 70-71 -0.4342229164636784 1106 72-73 0.14382097308426367 1106 74-75 0.18991080790734216 1106 76-77 0.1450837839646546 1106 78-79 0.3336860446736587 1106 80-81 -0.3588025967428585 1106 82-83 0.4490196467013625 1106 84-85 0.3076267435857538 1106 86-87 0.43808566445375163 1106 88-89 0.5787693306032367 1106 90-91 0.41103522641097 1106 92-93 0.2807927084375841 1106 94-95 0.27549872442000733 1106 96-97 0.5154353948837347 1106 98-99 0.8402588383626757 1106 100-101 1.1172256781555383 1107 1 0.14915327915464616 1107 2 0.20252025637952187 1107 3 0.30319455194460687 1107 4 0.15608049954720116 1107 5 0.0598517026337646 1107 6 0.15380974342134124 1107 7 0.18361241300902975 1107 8 0.09401742324405404 1107 9 0.38720706022380114 1107 10-11 0.2457994820877616 1107 12-13 0.27982314816398457 1107 14-15 0.1395013847138884 1107 16-17 0.24271685043473923 1107 18-19 0.2068158226735548 1107 20-21 0.3265156412593342 1107 22-23 0.3422589035396655 1107 24-25 0.36560765663048755 1107 26-27 0.29287415961860574 1107 28-29 0.3336550500102575 1107 30-31 0.3998280027985075 1107 32-33 0.3522737949506407 1107 34-35 0.3055883655198386 1107 36-37 0.32200501092885503 1107 38-39 0.1810121835862475 1107 40-41 0.0350218448668187 1107 42-43 0.2696880311707872 1107 44-45 0.2762725766801921 1107 46-47 0.2601208788747016 1107 48-49 0.41919089256924735 1107 50-51 0.4562030332237583 1107 52-53 0.3596360999465631 1107 54-55 0.569092236720536 1107 56-57 0.5198389434312958 1107 58-59 0.4855602342373615 1107 60-61 0.37953334704644703 1107 62-63 0.5009811155975257 1107 64-65 0.38615795648780704 1107 66-67 0.32938268783520286 1107 68-69 0.45154471379950323 1107 70-71 0.5075162670796125 1107 72-73 0.4632330265314799 1107 74-75 0.40292720083081335 1107 76-77 0.7647021344479406 1107 78-79 1.0579006116873977 1107 80-81 0.7889056286728469 1107 82-83 0.8290936602871071 1107 84-85 0.9127941224145211 1107 86-87 1.0326158523604931 1107 88-89 0.9896600949654264 1107 90-91 0.9026544093208884 1107 92-93 1.0295876125388688 1107 94-95 1.01479310712363 1107 96-97 0.4973697010403022 1107 98-99 1.1222377064886544 1107 100-101 1.5316644348539725 1108 1 0.19861480317400293 1108 2 0.19666386354653298 1108 3 0.18425254810368585 1108 4 0.11388687439706047 1108 5 0.06999916429802511 1108 6 0.1225756483120719 1108 7 0.07614568179913306 1108 8 0.1556237804500853 1108 9 0.14641408337114115 1108 10-11 0.2376720122349525 1108 12-13 0.2743569553169749 1108 14-15 0.39472158191756535 1108 16-17 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2316 68-69 -1.0315813849834825 2316 70-71 -1.2861168575292972 2316 72-73 -1.1871156042731776 2316 74-75 -0.935677807704046 2316 76-77 -1.2793130367387242 2316 78-79 -1.3352306378071113 2316 80-81 -1.255720391359592 2316 82-83 -1.7003430817388647 2316 84-85 -1.4529021388499999 2316 86-87 -1.2073349391889323 2316 88-89 -1.2078484079653755 2316 90-91 -1.1888462781501126 2316 92-93 -1.742724333280755 2316 94-95 -1.650017955412821 2316 96-97 -1.9872633001217999 2316 98-99 -2.156415786843091 2316 100-101 -2.099508437497775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 9.0 5 30.0 6 101.0 7 184.0 8 459.0 9 704.0 10 1246.0 11 1946.0 12 2688.0 13 3758.0 14 4733.0 15 6383.0 16 8180.0 17 10619.0 18 9172.0 19 6495.0 20 6086.0 21 6311.0 22 6963.0 23 7521.0 24 8248.0 25 9364.0 26 10254.0 27 12369.0 28 14819.0 29 17419.0 30 20649.0 31 24699.0 32 29681.0 33 36657.0 34 47051.0 35 62797.0 36 84585.0 37 115398.0 38 65090.0 39 2624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.158371713890766 26.261444431358207 16.824166423882524 19.756017430868507 2 14.332297558797261 24.607708147266052 40.42916418576957 20.630830108167114 3 16.00296299510455 30.70713613815739 33.83796198006177 19.451938886676285 4 10.731714120562287 22.00953675307186 41.64619792869286 25.612551197672996 5 9.745974256326612 41.62338326816275 36.92505434327585 11.705588132234787 6 23.655920643800567 43.731452224028466 19.016942691149602 13.595684441021366 7 22.540458365424698 37.40285420731358 24.513205629070825 15.5434817981909 8 23.603386368672837 36.46777510371258 22.009071847982234 17.919766679632353 9 24.637180319637746 18.324079139243725 24.538310503910626 32.500430037207906 10-11 21.277156268547774 30.144755948073197 29.902462912196476 18.675624871182546 12-13 22.691242582826714 29.608565411371266 29.495128569502537 18.20506343629948 14-15 21.02982676086677 30.706593748886164 28.31349480003657 19.950084690210492 16-17 17.278662561038164 33.576375382965566 30.034418473468644 19.11054358252763 18-19 17.11323383331293 31.49429793907574 33.897779768260314 17.494688459351025 20-21 17.706917632765272 30.878531147866163 33.95403328410505 17.460517935263518 22-23 18.169188260216675 30.6033073348076 33.680204186315365 17.54730021866036 24-25 17.801284455423755 32.04583001422696 33.52516993308062 16.627715597268672 26-27 17.314846311261054 31.436900019603847 34.33137320688723 16.91688046224787 28-29 16.535602071928622 30.723664074818785 35.15870709794395 17.58202675530864 30-31 17.51968702225604 30.572204086408266 34.65311090982209 17.254997981513608 32-33 17.232147976649294 31.378711310091106 34.26717558434877 17.121965128910833 34-35 17.70291842965856 31.202166736411403 34.28217169603695 16.812743137893086 36-37 18.042977702032942 30.461126391830717 33.56242787513262 17.933468031003716 38-39 16.817858130825623 31.77546847140742 33.68507594184391 17.721597455923046 40-41 16.791107226540706 30.95369819367476 34.7637024088469 17.49149217093763 42-43 17.141378287008948 30.647394606891666 34.12120042802693 18.090026678072455 44-45 17.11834369505466 30.570552047405357 33.89782306989532 18.413281187644664 46-47 18.184917549082353 30.930832970449085 32.727303720945365 18.156945759523193 48-49 17.357851394637784 30.873339707698673 32.810754184533224 18.958054713130316 50-51 16.749226509961808 30.81368904964361 32.94451560113716 19.49256883925742 52-53 17.23131972607792 31.751080516912474 32.584422890066996 18.433176866942613 54-55 17.591283238194567 31.130406618465777 32.1475532923024 19.130756851037255 56-57 17.703585812956284 31.364279482343683 31.75859648252809 19.173538222171942 58-59 17.294368201277827 30.795479556945743 32.540013454068614 19.370138787707816 60-61 17.78943828617388 31.914029750826366 31.456253205908013 18.84027875709174 62-63 17.12352438856179 31.488312119037694 32.24260392628491 19.145559566115605 64-65 17.344413067535438 31.15156183644812 31.873428786643565 19.630596309372876 66-67 17.245467500133667 31.28275344765996 30.90848368052085 20.563295371685523 68-69 17.21617143556829 30.637629090480743 31.539150664846005 20.60704880910496 70-71 17.678713390661297 30.773385113429093 31.6691797369567 19.87872175895291 72-73 17.704529341345204 29.88226269482444 31.811232890510894 20.601975073319466 74-75 16.933040066837478 31.103066432921487 30.92799005497972 21.03590344526131 76-77 16.93014926761579 30.65796358815937 31.889082261921835 20.52280488230301 78-79 17.047472374502306 30.11417583124516 31.540850548324567 21.297501245927965 80-81 17.162060464110525 30.163231552261454 31.061815008374328 21.612892975253693 82-83 17.27577351751358 30.179914472608218 31.147039057818965 21.397272952059236 84-85 16.75524618596584 29.781627848222907 31.148759344248067 22.314366621563185 86-87 16.481810820130725 30.02003153901888 31.564436075368178 21.93372156548221 88-89 17.23292124392994 29.643760575591344 30.966649761230098 22.156668419248625 90-91 17.02726414553278 29.204598121627633 31.650682882444396 22.11745485039519 92-93 17.335179930884344 29.32246041815049 31.12700059908502 22.215359051880142 94-95 16.86419975074559 28.425930518783986 31.635701043520314 23.074168686950106 96-97 17.326399600656682 28.425926930714862 31.110177518645582 23.13749594998287 98-99 16.78082218330469 28.559888426064823 30.707795213143275 23.951494177487216 100-101 16.506756374519433 28.388552174462244 31.567074366229054 23.537617084789265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2493.0 1 1544.0 2 641.5 3 714.0 4 641.0 5 645.5 6 1067.5 7 1989.5 8 2772.5 9 2957.5 10 2409.0 11 1679.5 12 1494.0 13 1443.0 14 1375.5 15 1352.0 16 1389.5 17 1435.0 18 1540.0 19 1649.0 20 1784.0 21 2017.0 22 2199.5 23 2376.0 24 2628.5 25 3322.5 26 5038.0 27 7064.0 28 7726.0 29 8677.0 30 11429.5 31 13986.5 32 17334.5 33 22548.5 34 28359.0 35 32521.0 36 37644.5 37 42848.5 38 42036.0 39 39179.5 40 36441.0 41 33032.5 42 29847.0 43 27861.5 44 26635.0 45 25269.0 46 22194.5 47 18380.0 48 16767.5 49 14105.5 50 10035.0 51 6281.0 52 3598.0 53 2293.0 54 1603.0 55 1255.0 56 1059.5 57 944.0 58 967.0 59 1017.5 60 1035.5 61 1063.0 62 887.5 63 557.0 64 305.0 65 212.5 66 160.5 67 126.0 68 110.0 69 88.5 70 69.0 71 60.0 72 50.0 73 49.0 74 41.0 75 40.0 76 35.0 77 23.0 78 19.5 79 21.0 80 19.5 81 13.0 82 7.5 83 3.0 84 1.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.5496836320865094E-4 2 0.0565634525711576 3 0.0 4 0.0 5 0.049434907863559654 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006043766165137387 26-27 0.0017821361768994859 28-29 7.748418160432547E-5 30-31 0.0011622627240648821 32-33 3.099367264173019E-4 34-35 0.012862374146318028 36-37 0.022392928483650064 38-39 0.004571566714655203 40-41 7.748418160432547E-4 42-43 0.0010072943608562313 44-45 0.007670933978828223 46-47 0.0 48-49 0.0 50-51 5.423892712302784E-4 52-53 0.0 54-55 0.0011622627240648821 56-57 0.0 58-59 0.02092072903316788 60-61 0.0 62-63 0.00805835488684985 64-65 0.014102121051987236 66-67 0.00565634525711576 68-69 0.016426646500117003 70-71 0.0 72-73 7.748418160432547E-5 74-75 0.02262538102846304 76-77 0.04811767677628612 78-79 0.029676441554456656 80-81 0.02580223247424038 82-83 0.03665001789884595 84-85 0.017046519952951606 86-87 0.009375585974123383 88-89 0.082133232500585 90-91 0.024794938113384152 92-93 0.02208299175723276 94-95 0.026189653382262014 96-97 0.03626259699082432 98-99 0.052611759309336995 100-101 0.01355973178075696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 645293.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.019585615906053 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3980279337582 13.960000763247782 2 9.307106254680573 3.540346084944637 3 3.6951580142045173 2.1084112264639265 4 2.181994007963169 1.6600248739139796 5 1.4423279792503243 1.3716240243784155 6 1.021759929005602 1.1660070269174485 7 0.7551992451376454 1.0054503689974148 8 0.6191251347948318 0.9420402806551743 9 0.5319537585443295 0.9105786048903202 >10 5.184293381837239 21.331985903277754 >50 1.0337630012615329 13.809702400422392 >100 0.7730820237960556 27.861264392980534 >500 0.04724842716561773 5.699271772659455 >1k 0.007331652491216544 2.763025052246522 >5k 0.001629256109159232 1.8702672240042486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6436 0.9973763856108776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5635 0.8732467266807481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4291 0.6649692465283212 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4218 0.6536565560140898 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1911 0.29614454209173197 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1504 0.23307241826581102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.19851447327028188 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1247 0.19324554892118775 TruSeq Adapter, Index 11 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1226 0.18999121329380605 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.1779036809635313 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1007 0.1560531417511115 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 977 0.15140409085485199 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 973 0.1507842174020174 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 879 0.13621719126040419 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 849 0.13156814036414466 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 836 0.12955355164243218 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 812 0.12583431092542458 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 811 0.1256793425622159 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 790 0.12242500693483424 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 762 0.11808589276499204 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 759 0.11762098767536606 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 756 0.11715608258574012 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 751 0.11638124076969686 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 748 0.11591633568007091 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 739 0.11452162041119306 RNA PCR Primer, Index 11 (95% over 23bp) GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 717 0.11111231642060274 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 706 0.10940766442530758 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.10816791751963838 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 697 0.1080129491564297 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 685 0.10615332879792591 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5496836320865096E-4 0.0 14-15 0.0 0.0 0.0 1.5496836320865096E-4 0.0 16-17 0.0 0.0 0.0 3.8742090802162737E-4 0.0 18-19 0.0 0.0 0.0 7.748418160432547E-4 0.0 20-21 0.0 0.0 0.0 0.001782136176899486 0.0 22-23 0.0 0.0 0.0 0.006896092162784967 0.0 24-25 0.0 0.0 0.0 0.014799478686426167 0.0 26-27 0.0 0.0 0.0 0.026422105927074988 0.0 28-29 0.0 0.0 0.0 0.052844211854149975 0.0 30-31 0.0 0.0 0.0 0.11653620913290552 0.0 32-33 0.0 0.0 0.0 0.2074251541547793 0.0 34-35 0.0 0.0 0.0 0.3399231046981759 0.0 36-37 0.0 0.0 0.0 0.5467283854001206 0.0 38-39 0.0 0.0 0.0 0.8055255519585677 0.0 40-41 0.0 0.0 0.0 1.0699015795925262 0.0 42-43 0.0 0.0 0.0 1.333347797047233 0.0 44-45 0.0 0.0 0.0 1.642354713285283 0.0 46-47 0.0 0.0 0.0 2.026676254042737 0.0 48-49 0.0 0.0 0.0 2.417506466054955 0.0 50-51 0.0 0.0 0.0 2.8004332915435315 0.0 52-53 0.0 0.0 0.0 3.159804925824393 0.0 54-55 0.0 0.0 0.0 3.558770976905065 0.0 56-57 0.0 0.0 0.0 4.038010640127817 0.0 58-59 0.0 0.0 0.0 4.530190161678494 0.0 60-61 0.0 0.0 0.0 5.047707010613783 0.0 62-63 0.0 0.0 0.0 5.535547417994616 0.0 64-65 0.0 0.0 0.0 6.078169141769708 0.0 66-67 0.0 0.0 0.0 6.680066264472108 0.0 68-69 0.0 0.0 0.0 7.327834022684269 0.0 70-71 0.0 0.0 0.0 8.04921175342054 0.0 72-73 0.0 0.0 0.0 8.716350557033781 0.0 74-75 0.0 0.0 0.0 9.441137591760642 0.0 76-77 0.0 0.0 0.0 10.215746955259084 0.0 78-79 0.0 0.0 0.0 11.057535104208476 0.0 80-81 0.0 0.0 0.0 11.9661921018824 0.0 82-83 0.0 0.0 0.0 12.83804411329427 0.0 84-85 0.0 0.0 0.0 13.6805296198781 0.0 86-87 0.0 0.0 0.0 14.572682486870306 0.0 88-89 0.0 0.0 0.0 15.508226495560931 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 25 5.2527314E-5 76.06075 1 CGTCAGA 35 3.3991473E-6 67.911385 2 GTTGTAC 40 5.3837255E-4 47.504807 3 TAGACCC 90 1.1941665E-8 42.226494 5 TCGTATC 25 0.0016138568 38.00974 94-95 TTGGACG 25 0.0016175482 37.992065 16-17 CATGGCA 55 0.0025814327 34.538242 9 TGGGGCC 55 0.0025814327 34.538242 8 CCGGGTC 70 2.0548567E-4 33.921486 9 CTTTAGG 215 0.0 33.166023 2 AGGGGTG 175 3.6379788E-12 32.56463 8 TCTAGAC 135 1.1943484E-8 31.66987 3 CGTAATC 30 0.0039536715 31.662506 22-23 TACGTGT 30 0.0039536715 31.662506 36-37 TGGACGT 30 0.0039544213 31.661282 18-19 AGCTGAT 60 0.0039537186 31.660053 7 CCTTTAG 230 0.0 31.003023 1 GGTATCA 4540 0.0 30.260954 1 ATGCCGC 40 4.4872047E-4 29.695107 94-95 GTCAGAT 80 4.4888185E-4 29.690504 3 >>END_MODULE