##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653293 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.186564068496065 31.0 31.0 34.0 28.0 34.0 2 31.31638330733683 31.0 31.0 34.0 28.0 34.0 3 31.32450064519289 31.0 31.0 34.0 28.0 34.0 4 34.98007938245167 37.0 35.0 37.0 32.0 37.0 5 35.02420353501415 37.0 35.0 37.0 32.0 37.0 6 35.09051068969054 37.0 35.0 37.0 32.0 37.0 7 35.05018268984973 37.0 35.0 37.0 32.0 37.0 8 35.033712285299245 37.0 35.0 37.0 32.0 37.0 9 36.54324935365908 39.0 37.0 39.0 32.0 39.0 10-11 36.42098797935995 39.0 37.0 39.0 32.0 39.0 12-13 36.3352278380451 39.0 37.0 39.0 32.0 39.0 14-15 37.304094793607156 40.0 37.0 41.0 32.0 41.0 16-17 37.20145478368818 39.0 37.0 41.0 32.0 41.0 18-19 37.31058805160931 39.5 37.0 41.0 32.0 41.0 20-21 37.43563531217998 40.0 37.0 41.0 32.0 41.0 22-23 37.35477649385498 40.0 37.0 41.0 32.0 41.0 24-25 37.24744180635641 40.0 37.0 41.0 31.5 41.0 26-27 37.08095601820317 39.5 36.0 41.0 31.0 41.0 28-29 36.92339348500596 39.0 36.0 41.0 31.0 41.0 30-31 36.66471629115879 39.0 35.5 41.0 30.5 41.0 32-33 36.331479902585826 39.0 35.0 40.5 30.0 41.0 34-35 36.09635416268045 38.0 35.0 40.0 30.0 41.0 36-37 35.802037523745085 38.0 35.0 40.0 29.5 41.0 38-39 35.56460730483872 38.0 35.0 40.0 28.5 41.0 40-41 35.44965658594229 38.0 35.0 40.0 27.0 41.0 42-43 35.20739469120288 38.0 35.0 40.0 26.5 41.0 44-45 34.83082246403987 38.0 34.5 40.0 24.5 41.0 46-47 34.3499295109545 38.0 34.0 40.0 22.5 41.0 48-49 33.91595271953013 38.0 33.0 40.0 19.0 41.0 50-51 33.18874149883743 37.5 33.0 39.5 15.0 40.5 52-53 33.29208257244453 38.0 33.0 39.5 15.0 40.5 54-55 33.49479023960153 38.0 33.0 40.0 12.0 41.0 56-57 33.285218883410664 38.0 33.0 40.0 8.5 41.0 58-59 33.04972424318032 38.0 33.0 40.0 7.5 41.0 60-61 32.709658606475195 38.0 32.5 40.0 2.0 41.0 62-63 32.29475518641712 37.0 32.0 40.0 2.0 41.0 64-65 31.90226131307086 36.5 31.0 39.0 2.0 41.0 66-67 31.4210185934948 36.0 31.0 39.0 2.0 40.5 68-69 30.922850849465707 35.5 30.0 39.0 2.0 40.0 70-71 30.382201401208953 35.0 29.0 38.0 2.0 40.0 72-73 29.869920541012988 35.0 29.0 37.5 2.0 39.5 74-75 29.30027797634446 34.5 28.5 37.0 2.0 39.0 76-77 28.63953922665634 34.0 27.5 36.0 2.0 39.0 78-79 27.907044771641516 34.0 26.0 36.0 2.0 38.0 80-81 27.266389659769814 33.5 26.0 35.0 2.0 37.0 82-83 26.6397030122778 33.0 24.5 35.0 2.0 37.0 84-85 26.006426672258847 32.5 22.0 35.0 2.0 36.0 86-87 25.412981617742727 32.0 20.0 35.0 2.0 36.0 88-89 24.685001216911857 31.5 15.0 34.5 2.0 35.0 90-91 23.935580206737253 31.0 4.5 34.0 2.0 35.0 92-93 23.18902621029155 31.0 2.0 34.0 2.0 35.0 94-95 22.31673460453426 30.0 2.0 34.0 2.0 35.0 96-97 21.183643480031165 29.0 2.0 34.0 2.0 35.0 98-99 19.87702914312567 29.0 2.0 34.0 2.0 35.0 100-101 16.306299011316515 13.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6849051345784751 1101 2 0.6275533457858771 1101 3 0.5963735357452968 1101 4 0.6148289926643073 1101 5 0.7082818072385848 1101 6 0.6442217114683828 1101 7 0.3987469173931686 1101 8 0.6562928172634344 1101 9 0.8351365271233391 1101 10-11 0.84356832158673 1101 12-13 0.7935109751427447 1101 14-15 0.8540544895274351 1101 16-17 1.1278237702521636 1101 18-19 0.9578201713540722 1101 20-21 0.8917325797083464 1101 22-23 0.9582845222428205 1101 24-25 1.0390783529309005 1101 26-27 0.8994042645244988 1101 28-29 0.978868179133265 1101 30-31 1.0588998258355602 1101 32-33 1.128119360604309 1101 34-35 1.0907973674843774 1101 36-37 1.2099294896676582 1101 38-39 1.1313997490785326 1101 40-41 1.1627081218184543 1101 42-43 0.9521074511250447 1101 44-45 0.8482485989001702 1101 46-47 0.6371113906137467 1101 48-49 0.6569541924895361 1101 50-51 0.8540115037766043 1101 52-53 1.0100392942472922 1101 54-55 1.0320603931743833 1101 56-57 0.7230773688591441 1101 58-59 0.8971277502772992 1101 60-61 0.9389739952346687 1101 62-63 0.9159214786415077 1101 64-65 0.9216987204251943 1101 66-67 0.7766425252756619 1101 68-69 0.7824518462876782 1101 70-71 0.8071840715703296 1101 72-73 0.6149971289209581 1101 74-75 0.5515962808414443 1101 76-77 0.784157191942068 1101 78-79 0.4086890943982695 1101 80-81 0.679538635399421 1101 82-83 0.7118985631216859 1101 84-85 0.8333421777013648 1101 86-87 1.1350353165099953 1101 88-89 1.2179432057902453 1101 90-91 1.4101707345637742 1101 92-93 1.3763242761806787 1101 94-95 1.3669337096234884 1101 96-97 1.5121241937719674 1101 98-99 1.8548769373649279 1101 100-101 1.341788695233884 1102 1 0.44680197669485366 1102 2 0.32992505841371766 1102 3 0.16910359239818362 1102 4 0.16079808437017817 1102 5 0.3093831858097502 1102 6 0.38187608134361994 1102 7 0.2760535557597876 1102 8 0.2552083342408267 1102 9 0.35505479490722536 1102 10-11 0.49033256004628356 1102 12-13 0.413363772676 1102 14-15 0.2977421541930312 1102 16-17 0.3290418337055101 1102 18-19 0.4971419206769383 1102 20-21 0.21072317627960757 1102 22-23 0.41475472535899627 1102 24-25 0.2932679209856417 1102 26-27 0.464691040061858 1102 28-29 0.3665418204935733 1102 30-31 0.24832532438623645 1102 32-33 0.3388042111051064 1102 34-35 0.22617559017826494 1102 36-37 0.3209306037178834 1102 38-39 0.4455091160762521 1102 40-41 0.45113361508927596 1102 42-43 0.5469350373319415 1102 44-45 0.5657748265993661 1102 46-47 0.29131759022408943 1102 48-49 0.35910943090156167 1102 50-51 0.4905446956097208 1102 52-53 0.4537507182780445 1102 54-55 0.5227889609070573 1102 56-57 0.5965455497893473 1102 58-59 0.7274990827553012 1102 60-61 0.6450429290727584 1102 62-63 0.30254706800575804 1102 64-65 0.4648114084541781 1102 66-67 0.3309458742901725 1102 68-69 0.026482965776168044 1102 70-71 0.12758439047380676 1102 72-73 0.053168660977622295 1102 74-75 -0.1531540082308318 1102 76-77 -0.07711055498373298 1102 78-79 0.13615993637736779 1102 80-81 0.3455136405472814 1102 82-83 0.3541920810388888 1102 84-85 0.0984742533723022 1102 86-87 0.1977508007522566 1102 88-89 0.6044697977997053 1102 90-91 0.6368865567170054 1102 92-93 0.8057338823560016 1102 94-95 0.8358049813831094 1102 96-97 1.1887975130608766 1102 98-99 1.4844974739041916 1102 100-101 0.867224415591437 1103 1 0.46137810323970996 1103 2 0.32678835928792793 1103 3 0.2255768758491108 1103 4 0.2014405616259367 1103 5 0.12403601544254172 1103 6 0.12929941635324838 1103 7 0.004097931797950594 1103 8 0.18449926244978343 1103 9 0.19767631342517689 1103 10-11 0.1256677931779464 1103 12-13 0.17000417742448093 1103 14-15 0.35276265068043955 1103 16-17 0.4349428918017466 1103 18-19 0.45131817522959494 1103 20-21 0.5670140994088868 1103 22-23 0.44706653611063274 1103 24-25 0.4651984005577319 1103 26-27 0.5899621676988502 1103 28-29 0.28299895057896407 1103 30-31 0.4388055060354006 1103 32-33 0.5190056710036117 1103 34-35 0.5090652139267533 1103 36-37 0.5773525824543668 1103 38-39 0.47749582764723897 1103 40-41 0.4975745410123764 1103 42-43 0.33011039278218135 1103 44-45 0.5100825431331941 1103 46-47 0.4645497364374478 1103 48-49 0.5158402538596718 1103 50-51 0.7133100197183637 1103 52-53 0.6585657419239226 1103 54-55 0.5044259368751156 1103 56-57 0.47525053752193 1103 58-59 0.5194127486041396 1103 60-61 0.5418658465331205 1103 62-63 0.367500868364381 1103 64-65 0.20096548514710477 1103 66-67 0.0587108682199613 1103 68-69 0.1735560572153929 1103 70-71 0.18869287708632143 1103 72-73 0.24387361823216125 1103 74-75 0.1423713063280232 1103 76-77 0.20182581193132876 1103 78-79 0.30298788271568355 1103 80-81 0.29938003494338616 1103 82-83 0.46261646754117436 1103 84-85 0.23058262315230138 1103 86-87 0.4448198284517204 1103 88-89 0.3974387660669798 1103 90-91 0.6474916475669872 1103 92-93 0.9068536966430933 1103 94-95 1.185722182685538 1103 96-97 1.7213087010444 1103 98-99 2.169128410139038 1103 100-101 1.6436120557269653 1104 1 0.4635118933299225 1104 2 0.4100376232271188 1104 3 0.6027908040308638 1104 4 0.5626534833942216 1104 5 0.6181584156418864 1104 6 0.7007801747438549 1104 7 0.5963117753054945 1104 8 0.3214029443263584 1104 9 0.68260067516308 1104 10-11 0.5443745034881218 1104 12-13 0.6925722736337363 1104 14-15 0.5804467626536152 1104 16-17 0.6752894270540395 1104 18-19 0.8494042961588804 1104 20-21 0.7411417736018819 1104 22-23 0.7345451558726239 1104 24-25 0.6401691879553084 1104 26-27 0.6621287871898858 1104 28-29 0.7782971126647524 1104 30-31 0.6992222483040464 1104 32-33 0.6978593757422189 1104 34-35 0.7006490813124273 1104 36-37 0.5975933475221495 1104 38-39 0.8478027595619224 1104 40-41 0.8821248846687055 1104 42-43 0.8375169668878399 1104 44-45 1.1324113742178241 1104 46-47 1.3475384457363688 1104 48-49 1.194836695123584 1104 50-51 0.8992630521437448 1104 52-53 0.80960275906925 1104 54-55 1.0636023229314517 1104 56-57 1.3001273365464812 1104 58-59 1.1822146424033235 1104 60-61 1.4164972113120413 1104 62-63 1.1999560091775905 1104 64-65 1.133550298464666 1104 66-67 1.201742543632264 1104 68-69 1.1769793145596736 1104 70-71 1.1239134775958206 1104 72-73 1.0983730847846367 1104 74-75 0.7962533769269626 1104 76-77 1.0642048185335007 1104 78-79 0.9774748174850956 1104 80-81 1.1779959261152513 1104 82-83 1.3570615974435114 1104 84-85 1.2118478493896951 1104 86-87 1.2021689450577853 1104 88-89 1.4144280414734745 1104 90-91 1.2054656598423286 1104 92-93 1.2544703595599032 1104 94-95 1.513366618743202 1104 96-97 1.7741333117244764 1104 98-99 1.711980818152604 1104 100-101 0.9151924877589757 1105 1 0.30083486932266723 1105 2 0.3988043193495905 1105 3 0.39704969744864727 1105 4 0.37434759331219425 1105 5 0.36362785022917876 1105 6 0.37470206904531267 1105 7 0.34335424256097014 1105 8 0.2808395210371444 1105 9 0.6300035519622327 1105 10-11 0.4506714920558821 1105 12-13 0.4333630926748313 1105 14-15 0.5357425621937253 1105 16-17 0.59962886899757 1105 18-19 0.453437274326113 1105 20-21 0.32728266181015186 1105 22-23 0.4513703595572238 1105 24-25 0.6224054852214636 1105 26-27 0.6372899073742033 1105 28-29 0.500254335429311 1105 30-31 0.42086833445996064 1105 32-33 0.2810866835893435 1105 34-35 0.5706228949450676 1105 36-37 0.5515821419776685 1105 38-39 0.6001923812475596 1105 40-41 0.4195329894881965 1105 42-43 0.3475392669391866 1105 44-45 0.3618983342396618 1105 46-47 0.2635419711801674 1105 48-49 0.2224607680752868 1105 50-51 0.11783362810503206 1105 52-53 0.043549729362062806 1105 54-55 0.1074563917655027 1105 56-57 0.19916986857388963 1105 58-59 0.3193356687608784 1105 60-61 0.44132915127770644 1105 62-63 0.3754844787556024 1105 64-65 0.2924578272766034 1105 66-67 0.3580002063833163 1105 68-69 0.20450969953628473 1105 70-71 0.3395820396126332 1105 72-73 0.12961050630650206 1105 74-75 -0.21842440583722578 1105 76-77 0.11310762824743748 1105 78-79 0.2548201397948624 1105 80-81 0.16821305088912553 1105 82-83 0.08301733045360038 1105 84-85 -0.171440066480816 1105 86-87 -0.11647773792523708 1105 88-89 0.04239540567217759 1105 90-91 -0.04719461599357544 1105 92-93 0.02179453845998225 1105 94-95 -0.0321703637174835 1105 96-97 0.1333434179967128 1105 98-99 0.3369906496067827 1105 100-101 0.3071633126295481 1106 1 0.2322714645397248 1106 2 0.33653073195606 1106 3 0.11887043649640816 1106 4 0.15683418783526548 1106 5 0.17711706801554783 1106 6 -0.01591201514894891 1106 7 5.996772963072772E-4 1106 8 0.18510891676127272 1106 9 0.3104720381033559 1106 10-11 0.1714801696443331 1106 12-13 0.2745018295295978 1106 14-15 0.1991297641850025 1106 16-17 0.12015925860075072 1106 18-19 -0.01966155376454992 1106 20-21 0.46931941525578935 1106 22-23 0.22459109767967078 1106 24-25 0.1761569368067626 1106 26-27 0.17361423895098227 1106 28-29 0.5922327941348939 1106 30-31 0.2436326617803033 1106 32-33 0.40536320422614125 1106 34-35 0.3109094182190191 1106 36-37 0.06083990188661659 1106 38-39 0.1579099571116913 1106 40-41 0.3216348387269008 1106 42-43 0.1797834293686904 1106 44-45 0.18677584588704832 1106 46-47 0.016757435016607758 1106 48-49 0.10754726173920659 1106 50-51 -0.1933862587291486 1106 52-53 0.037014703939078686 1106 54-55 0.030677361216284282 1106 56-57 -0.24581447049792615 1106 58-59 -0.4640334433547153 1106 60-61 -0.075080124063156 1106 62-63 0.29107392648234054 1106 64-65 0.45361155420738797 1106 66-67 0.05626015465829681 1106 68-69 -0.02975881714017703 1106 70-71 -0.046836905252572336 1106 72-73 0.4391081536973509 1106 74-75 0.39349686850429677 1106 76-77 0.32071324119812417 1106 78-79 0.5103997792002559 1106 80-81 0.2752394176925854 1106 82-83 0.5469543998937425 1106 84-85 0.33053085092108603 1106 86-87 0.4831796648127664 1106 88-89 -0.15525036587721175 1106 90-91 0.17713487883160894 1106 92-93 0.08448567349558189 1106 94-95 0.3265366034292647 1106 96-97 -0.026785524416411022 1106 98-99 -0.21221520022411866 1106 100-101 -0.4745471753344397 1107 1 0.33884776812492134 1107 2 0.5133165432145184 1107 3 0.5114697870117588 1107 4 0.39397862757329705 1107 5 0.37876358762498796 1107 6 0.38921000759388136 1107 7 0.29626290104268094 1107 8 0.2710711423865888 1107 9 0.510243958210225 1107 10-11 0.47374684934413125 1107 12-13 0.4960369158198219 1107 14-15 0.6125354314273039 1107 16-17 0.5726827997040047 1107 18-19 0.5656739952020331 1107 20-21 0.5872117398243333 1107 22-23 0.5201229039638164 1107 24-25 0.5701931906488227 1107 26-27 0.7022306628754791 1107 28-29 0.4470371341694275 1107 30-31 0.5566603680568463 1107 32-33 0.4014869321376935 1107 34-35 0.4142784840689373 1107 36-37 0.43803625695269943 1107 38-39 0.45170509792387037 1107 40-41 0.46466808408656135 1107 42-43 0.1827506401688268 1107 44-45 0.3514593035782241 1107 46-47 0.12232258440894128 1107 48-49 0.13737902566565907 1107 50-51 0.1720878759248734 1107 52-53 0.05410874888167427 1107 54-55 0.12784699835203384 1107 56-57 0.311733344822521 1107 58-59 0.16184493575750736 1107 60-61 0.26311891365691764 1107 62-63 0.2867002230079194 1107 64-65 0.1268099945753569 1107 66-67 0.25635464489973714 1107 68-69 0.11546725347770348 1107 70-71 0.1568015120901869 1107 72-73 0.2608343926599126 1107 74-75 0.41874857095778495 1107 76-77 0.45938690231484713 1107 78-79 0.6283550972630962 1107 80-81 0.6390257611374643 1107 82-83 0.725115207079341 1107 84-85 0.6089631817889831 1107 86-87 0.3788287693276615 1107 88-89 0.6810393173089544 1107 90-91 0.6766906660873744 1107 92-93 0.8292327763378928 1107 94-95 0.7175328781243486 1107 96-97 0.541422310670967 1107 98-99 1.1402453473848446 1107 100-101 0.5357131615721435 1108 1 0.7262428562354231 1108 2 0.6220966231178906 1108 3 0.5300395785816434 1108 4 0.5399478430956108 1108 5 0.6956331097532669 1108 6 0.6412029463655955 1108 7 0.6085116544881259 1108 8 0.6172137915860105 1108 9 0.4251437692914024 1108 10-11 0.4647097159550455 1108 12-13 0.47078992768145156 1108 14-15 0.5605208134459474 1108 16-17 0.5680651280511242 1108 18-19 0.646683007559183 1108 20-21 0.8392913954346071 1108 22-23 0.8113366300005467 1108 24-25 0.6520848565412365 1108 26-27 0.6783711441539353 1108 28-29 0.5688216428256325 1108 30-31 0.5646290261137707 1108 32-33 0.6074146474587039 1108 34-35 0.9695914389452085 1108 36-37 0.8937084673029716 1108 38-39 0.7831554370486487 1108 40-41 0.9354940619737633 1108 42-43 1.0101810344207323 1108 44-45 1.1944027208410688 1108 46-47 1.3947497267794162 1108 48-49 1.1022865793216994 1108 50-51 1.2630971979850258 1108 52-53 1.2632140169296235 1108 54-55 1.2684490609739854 1108 56-57 1.0284387243635464 1108 58-59 1.1183006946846987 1108 60-61 1.4295842733769462 1108 62-63 1.6559312304717722 1108 64-65 1.430281765147381 1108 66-67 1.3525669772729927 1108 68-69 1.3446412755904973 1108 70-71 1.5047590208392982 1108 72-73 1.344627634940565 1108 74-75 1.592166762911699 1108 76-77 1.8536604939404775 1108 78-79 1.4879047891086188 1108 80-81 1.4545294930585477 1108 82-83 1.347327105868402 1108 84-85 1.5683827983766783 1108 86-87 1.769029766571304 1108 88-89 1.711418084071628 1108 90-91 1.6337565399143728 1108 92-93 1.8007153245741385 1108 94-95 1.5755619282652624 1108 96-97 1.6159740155859552 1108 98-99 1.4047224138780763 1108 100-101 0.8380421280932531 1109 1 0.4568633431492586 1109 2 0.6164454543534426 1109 3 0.4955331996108434 1109 4 0.6038659867722984 1109 5 0.55451800286626 1109 6 0.505139641858932 1109 7 0.5127557393127375 1109 8 0.3671495737591357 1109 9 0.4984305803768265 1109 10-11 0.4428471135632961 1109 12-13 0.2676368323821947 1109 14-15 0.3812567497418584 1109 16-17 0.42702850961916994 1109 18-19 0.3864117229172308 1109 20-21 0.2080016160585174 1109 22-23 0.0901062164377393 1109 24-25 0.24939983784670972 1109 26-27 0.3783918365648091 1109 28-29 0.08977806032712721 1109 30-31 0.3227846901260989 1109 32-33 0.33194514379114537 1109 34-35 0.4257163925656684 1109 36-37 0.3316776142048212 1109 38-39 0.21965114158955856 1109 40-41 0.5274896666202835 1109 42-43 0.7364775209920751 1109 44-45 0.9446417538633085 1109 46-47 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-1.6522709216375198 2316 96-97 -1.7141867383532094 2316 98-99 -2.128455796685145 2316 100-101 -1.904432823829314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4968.0 3 918.0 4 717.0 5 650.0 6 685.0 7 841.0 8 1031.0 9 1230.0 10 1659.0 11 2308.0 12 3282.0 13 4350.0 14 5321.0 15 7203.0 16 8730.0 17 10819.0 18 9048.0 19 6386.0 20 6265.0 21 6514.0 22 7149.0 23 7854.0 24 8804.0 25 9941.0 26 11615.0 27 13328.0 28 15775.0 29 18467.0 30 21842.0 31 25467.0 32 30986.0 33 37327.0 34 47598.0 35 62509.0 36 79578.0 37 103509.0 38 64217.0 39 4402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.524436810869666 25.402321765400483 16.588661074391574 19.48458034933828 2 14.256396119062387 24.27316654797185 41.07039751276926 20.400039820196497 3 16.215545679254355 30.517515460223045 33.543553569404835 19.72338529111776 4 10.826391473420296 21.272033608863524 42.29013222079786 25.611442696918317 5 9.967620588037079 41.127520355836566 36.9154398024764 11.98941925364996 6 23.732611605843665 43.60202059190231 19.301921645391634 13.363446156862386 7 22.829530785097706 37.24547377060979 24.883197785083926 15.041797659208578 8 23.55186813489712 36.15019807635472 22.207674673943504 18.090259114804656 9 25.087786195247524 18.25769095890243 24.956240897877052 31.698281947972994 10-11 21.410664980084135 30.03298636900323 30.03536003993893 18.52098861097371 12-13 23.040075955957796 29.698626361005193 29.46095771887107 17.80033996416594 14-15 21.329727693549202 30.599600950002376 28.78504129080271 19.285630065645716 16-17 17.31104776910845 33.282726084629715 30.447954540756445 18.958271605505395 18-19 17.08962332050069 31.328883253550416 34.50274263347942 17.07875079246947 20-21 17.84256385635164 30.685583323570242 34.45467207886433 17.017180741213796 22-23 18.336387241122928 30.18353309438068 34.15917738214379 17.3209022823526 24-25 17.823800705507896 31.630832196035 34.099650309482996 16.445716788974103 26-27 17.333370089508946 31.04097318100962 34.70380265609314 16.9218540733883 28-29 16.8385137587423 30.41999807045297 35.51247084622888 17.229017324575842 30-31 17.71950379048932 30.392143349414198 34.729994639712075 17.15835822038441 32-33 17.421224194132563 30.946978949472147 34.563954445527514 17.067842410867772 34-35 17.861838352235093 30.82491617296968 34.45227774446902 16.860967730326205 36-37 18.225255136419882 30.125163863126815 33.767136713916415 17.882444286536884 38-39 16.983306002520255 31.580486565029727 33.837383958879755 17.59882347357026 40-41 16.90419575173056 30.80929333821253 34.989197887654974 17.29731302240194 42-43 17.19740298116694 30.579735995780343 34.11216849235264 18.110692530700078 44-45 17.241954680617372 30.483006188265637 34.00760043015526 18.267438700961726 46-47 18.1510257195346 30.8877066748316 33.07440293937538 17.886864666258422 48-49 17.506912103759635 30.852400941821976 33.01206977320951 18.628617181208877 50-51 16.908087205323806 30.73059947919298 33.12674042469807 19.234572890785145 52-53 17.469256099409233 31.5216642504275 32.75126718604327 18.257812464119997 54-55 17.795925803170046 30.968672330503967 32.44989922819437 18.785502638131625 56-57 17.754481255195408 31.13946122368999 32.269783120873335 18.836274400241265 58-59 17.674326750998524 30.22765927806091 32.93642349432727 19.161590476613295 60-61 18.081188720565393 31.107237910216757 32.21186561983079 18.59970774938706 62-63 17.548047852676213 30.602061981921302 32.92879503409847 18.921095131304007 64-65 17.736815798920603 30.38480055783949 32.60368213865006 19.274701504589853 66-67 17.668471709305216 30.551654197202506 31.699049004037427 20.08082508945485 68-69 17.68063848727442 30.02113339604719 32.258931175241855 20.039296941436536 70-71 18.181059600418294 30.395953044785674 32.25238963580159 19.170597718994447 72-73 18.286172284552446 29.548022512370782 32.489144946352454 19.676660256724315 74-75 17.5454014457728 30.91263738324768 31.733051823827925 19.808909347151594 76-77 17.696868098852185 30.52805621576935 32.35016974192339 19.42490594345508 78-79 17.99716800612323 30.07102946804439 31.87500956754688 20.056792958285495 80-81 18.197919752250133 30.157392503055924 31.411832872935573 20.23285487175837 82-83 18.450964033402474 30.160276772114596 31.498365850484888 19.890393343998042 84-85 18.123967663271852 29.846490858797004 31.43791590062625 20.591625577304896 86-87 18.065961049179926 29.998515080354053 31.647082056353465 20.288441814112552 88-89 18.94882063680789 29.600488756831993 31.332727491270283 20.117963115089832 90-91 18.82722429710481 29.557047020973926 31.56646744731253 20.049261234608736 92-93 19.483091498974943 29.486031175886072 31.225263460887753 19.80561386425123 94-95 19.3271951637798 28.783809442144204 31.442934316583777 20.44606107749222 96-97 19.89052981029914 28.4683743852925 30.89680876557776 20.744287038830603 98-99 19.63470005029592 28.70878815925357 30.440284504952654 21.216227285497858 100-101 20.06872025385421 28.4074111820604 31.021125654309845 20.50274290977555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2448.0 1 1522.0 2 685.5 3 764.0 4 653.5 5 646.5 6 1077.5 7 2087.5 8 3117.5 9 3362.0 10 2591.5 11 1763.5 12 1588.5 13 1567.5 14 1518.5 15 1497.0 16 1517.5 17 1612.5 18 1754.5 19 1889.0 20 2106.5 21 2334.5 22 2523.0 23 2806.0 24 3127.0 25 3819.0 26 5481.0 27 7468.0 28 8121.5 29 9062.5 30 11663.5 31 14245.0 32 17637.5 33 22601.0 34 28560.5 35 32879.5 36 37692.0 37 43113.5 38 42401.0 39 39468.0 40 36807.0 41 33146.5 42 30030.0 43 27889.0 44 26029.0 45 24111.5 46 21014.5 47 17340.5 48 15737.5 49 13283.0 50 9385.0 51 5996.5 52 3798.5 53 2678.0 54 2007.0 55 1656.5 56 1435.0 57 1311.5 58 1257.0 59 1247.0 60 1288.5 61 1304.0 62 1149.5 63 808.5 64 515.0 65 395.0 66 328.0 67 276.5 68 238.0 69 198.0 70 179.5 71 157.0 72 129.0 73 122.0 74 101.5 75 83.5 76 70.5 77 46.5 78 40.5 79 49.0 80 41.5 81 24.5 82 15.5 83 9.5 84 4.0 85 2.5 86 1.5 87 0.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1841440211359987 2 0.05479930138544267 3 0.050054110483351275 4 0.056636149476574825 5 0.06260590577275434 6 0.06352432981832042 7 0.04071679935342947 8 0.04010451665638542 9 0.04377821283864974 10-11 0.04530891958125986 12-13 0.0433190008158667 14-15 0.03704310317116516 16-17 0.03436436637159743 18-19 0.04148215272473454 20-21 0.04951836312343772 22-23 0.046610020312478476 24-25 0.06582038993223562 26-27 0.05227363526013595 28-29 0.04439049553569378 30-31 0.05250324127152747 32-33 0.04844686840361063 34-35 0.04768151503230557 36-37 0.04729883834665303 38-39 0.028165004064026403 40-41 0.02755272136698235 42-43 0.02441477254463158 44-45 0.006199362307571029 46-47 0.014235572706274215 48-49 0.013929431357752186 50-51 0.011633371243836992 52-53 0.011327229895314966 54-55 0.012934471975055602 56-57 0.011021088546792939 58-59 0.012245653940881044 60-61 0.013240613323577628 62-63 0.005816685621918496 64-65 0.008954634444269263 66-67 0.004668655564960898 68-69 0.009413846467052303 70-71 0.024491307881762087 72-73 0.021582965070802842 74-75 0.022577924453499425 76-77 0.014618249391926746 78-79 0.006582038993223561 80-81 0.008189281072964199 82-83 0.007347392364528626 84-85 0.006428968318962548 86-87 0.00903116978139977 88-89 0.03176216490916021 90-91 0.037349244519687186 92-93 0.024491307881762087 94-95 0.03382861901168389 96-97 0.03650735581125161 98-99 0.02426170187037057 100-101 0.12130850935185285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 653293.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.834561976676152 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02059615266934 16.817109801760033 2 8.163967506069634 3.565133089736952 3 3.271176258643245 2.1427410226793264 4 1.8445707256966377 1.6110157532234295 5 1.2196584850485026 1.3315354391085237 6 0.8622023711205813 1.1295486665201677 7 0.6398020812153244 0.9778858736571672 8 0.5272348389830579 0.9209553414430758 9 0.4801519741144281 0.9435517233322396 >10 4.411930519585629 20.91550273987468 >50 0.8578506844913754 13.068401475933122 >100 0.6602086150252251 27.445050969592096 >500 0.035042920118111734 4.989216296866994 >1k 0.004205150414173409 2.104153730697211 >5k 0.0014017168047244693 2.038198075574993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7338 1.1232326077273138 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5981 0.9155157027551191 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.6846851259695114 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3962 0.6064660114221336 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1488 0.22776916330038743 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.20572698620680155 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1297 0.19853266451653395 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.18154181967356148 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 986 0.15092768482135888 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 976 0.14939697807874874 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 944 0.14449871650239632 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 854 0.13072235581890515 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 812 0.12429338749994259 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 805 0.12322189278011551 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 795 0.12169118603750538 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 771 0.11801748985524106 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 766 0.11725213648393601 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 761 0.11648678311263093 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 754 0.11541528839280385 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.11373151097593269 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 734 0.11235387490758357 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 730 0.11174159221053953 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 727 0.11128238018775649 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 725 0.11097623883923446 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.10638411861140407 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 683 0.1045472705202719 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 674 0.1031696344519228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5307067426101304E-4 0.0 4 0.0 0.0 0.0 1.5307067426101304E-4 0.0 5 0.0 0.0 0.0 1.5307067426101304E-4 0.0 6 0.0 0.0 0.0 1.5307067426101304E-4 0.0 7 0.0 0.0 0.0 1.5307067426101304E-4 0.0 8 0.0 0.0 0.0 1.5307067426101304E-4 0.0 9 0.0 0.0 0.0 3.061413485220261E-4 0.0 10-11 0.0 0.0 0.0 3.061413485220261E-4 0.0 12-13 0.0 0.0 0.0 6.888180341745587E-4 0.0 14-15 0.0 0.0 0.0 9.184240455660783E-4 0.0 16-17 0.0 0.0 0.0 0.0010714947198270915 0.0 18-19 1.5307067426101304E-4 0.0 0.0 0.001148030056957598 0.0 20-21 1.5307067426101304E-4 0.0 0.0 0.0017603127540016502 0.0 22-23 1.5307067426101304E-4 0.0 0.0 0.005893220959049003 0.0 24-25 1.5307067426101304E-4 0.0 0.0 0.014082502032013201 0.0 26-27 1.5307067426101304E-4 0.0 0.0 0.02640469131002475 0.0 28-29 1.5307067426101304E-4 0.0 0.0 0.05135521121456988 0.0 30-31 1.5307067426101304E-4 0.0 0.0 0.11771134850671904 0.0 32-33 1.5307067426101304E-4 0.0 0.0 0.21713075143924704 0.0 34-35 1.5307067426101304E-4 0.0 0.0 0.3642316694040806 0.0 36-37 1.5307067426101304E-4 0.0 0.0 0.5726373924104499 0.0 38-39 1.5307067426101304E-4 0.0 0.0 0.8338524980368687 0.0 40-41 1.5307067426101304E-4 0.0 0.0 1.1070071162556463 0.0 42-43 1.5307067426101304E-4 0.0 0.0 1.377712603686248 0.0 44-45 1.5307067426101304E-4 0.0 0.0 1.6969414948575907 0.0 46-47 1.5307067426101304E-4 0.0 0.0 2.0912515517539605 0.0 48-49 1.5307067426101304E-4 0.0 0.0 2.4723975306638826 0.0 50-51 1.5307067426101304E-4 0.0 0.0 2.8510943787856293 0.0 52-53 1.5307067426101304E-4 0.0 0.0 3.224357217971109 0.0 54-55 1.5307067426101304E-4 0.0 0.0 3.613845548628257 0.0 56-57 1.5307067426101304E-4 0.0 0.0 4.081782599844174 0.0 58-59 1.5307067426101304E-4 0.0 0.0 4.5783438671469 0.0 60-61 1.5307067426101304E-4 0.0 0.0 5.094192039406514 0.0 62-63 1.5307067426101304E-4 0.0 0.0 5.583252843670451 0.0 64-65 1.5307067426101304E-4 0.0 0.0 6.125964919262873 0.0 66-67 1.5307067426101304E-4 0.0 0.0 6.740773282432231 0.0 68-69 1.5307067426101304E-4 0.0 0.0 7.386195780453793 0.0 70-71 1.5307067426101304E-4 0.0 0.0 8.108689362965775 0.0 72-73 1.5307067426101304E-4 0.0 0.0 8.77730206813788 0.0 74-75 1.5307067426101304E-4 0.0 0.0 9.51487311206457 0.0 76-77 1.5307067426101304E-4 0.0 0.0 10.290023006522341 0.0 78-79 1.5307067426101304E-4 0.0 0.0 11.133672027711913 0.0 80-81 1.5307067426101304E-4 0.0 0.0 12.041687267428244 0.0 82-83 1.5307067426101304E-4 0.0 0.0 12.894520529073478 0.0 84-85 1.5307067426101304E-4 0.0 0.0 13.719494928003208 0.0 86-87 1.5307067426101304E-4 0.0 0.0 14.594982649439073 0.0 88-89 1.5307067426101304E-4 0.0 0.0 15.49963033432166 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 25 0.004975594 57.12897 1 CGTCAGA 25 0.005010449 57.028313 2 GCTAAGC 50 0.0015974671 38.08598 1 GTTCGCG 25 0.0016162103 37.9985 84-85 GGTATCA 4215 0.0 33.99727 1 GTATGGT 90 2.4032062E-5 31.682396 3 GATCCTC 75 3.0735717E-4 31.672691 6 TGTACAG 80 4.4825146E-4 29.697697 5 ATGGGCG 65 0.0058324514 29.24081 5 GACCGCG 65 0.005841189 29.231852 7 GTATCAA 6290 0.0 28.836958 1 GGATATG 355 0.0 28.162169 1 CATATAG 220 1.8189894E-12 28.082123 3 GTAAGGT 170 3.616151E-9 27.955053 4 GTAAGCG 35 0.0083654225 27.1605 94-95 CCGGGTC 70 0.008390579 27.141785 9 GGTGGAC 35 0.008393599 27.141785 74-75 GGGATTC 70 0.008390579 27.141785 9 ACATATA 230 5.456968E-12 26.861162 2 CAGATAC 125 7.3867723E-6 26.598951 9 >>END_MODULE