##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 656887 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20213826731234 31.0 31.0 34.0 28.0 34.0 2 31.365046042926714 31.0 31.0 34.0 28.0 34.0 3 31.376574662004273 33.0 31.0 34.0 28.0 34.0 4 35.051290404590134 37.0 35.0 37.0 32.0 37.0 5 35.08984802561171 37.0 35.0 37.0 33.0 37.0 6 35.13157666387065 37.0 35.0 37.0 32.0 37.0 7 35.083515125128066 37.0 35.0 37.0 32.0 37.0 8 35.06337163012817 37.0 35.0 37.0 32.0 37.0 9 36.54414990706164 39.0 37.0 39.0 32.0 39.0 10-11 36.44651439288644 39.0 37.0 39.0 32.0 39.0 12-13 36.34087521902549 39.0 37.0 39.0 32.0 39.0 14-15 37.30529604026263 40.0 37.0 41.0 32.0 41.0 16-17 37.182539767113674 39.0 37.0 41.0 32.0 41.0 18-19 37.209927278207665 39.5 37.0 41.0 32.0 41.0 20-21 37.25259595638215 39.5 36.5 41.0 32.0 41.0 22-23 37.300184811086226 40.0 37.0 41.0 32.0 41.0 24-25 37.22015278122417 40.0 37.0 41.0 32.0 41.0 26-27 37.04894829704348 39.0 36.0 41.0 31.0 41.0 28-29 36.89905189172567 39.0 36.0 41.0 31.0 41.0 30-31 36.60445099385434 39.0 35.5 40.5 30.5 41.0 32-33 36.26990867531249 38.5 35.0 40.0 30.0 41.0 34-35 36.02572284121926 38.0 35.0 40.0 30.0 41.0 36-37 35.73584421673743 38.0 35.0 40.0 29.0 41.0 38-39 35.450190824297025 38.0 35.0 40.0 28.0 41.0 40-41 35.321660346452276 38.0 35.0 40.0 27.0 41.0 42-43 35.02938633280914 38.0 35.0 40.0 25.5 41.0 44-45 34.645810466640384 38.0 34.0 40.0 24.5 41.0 46-47 34.15087526469545 38.0 33.5 40.0 22.0 41.0 48-49 33.7174734771734 38.0 33.0 40.0 18.5 41.0 50-51 32.97179499670415 37.5 32.5 39.5 15.0 40.5 52-53 33.05090373230099 37.5 33.0 39.5 14.0 40.5 54-55 33.21221838763745 38.0 33.0 40.0 10.5 41.0 56-57 32.98016249370135 38.0 33.0 40.0 8.0 41.0 58-59 32.66742377303859 38.0 32.0 40.0 4.5 41.0 60-61 32.28882440967777 37.0 31.5 40.0 2.0 41.0 62-63 31.806366239551096 36.5 31.0 39.5 2.0 41.0 64-65 31.426207247212993 36.0 31.0 39.0 2.0 40.5 66-67 30.908443156889998 36.0 29.5 39.0 2.0 40.0 68-69 30.345361530978693 35.0 29.0 38.5 2.0 40.0 70-71 29.799232592515914 35.0 28.5 38.0 2.0 40.0 72-73 29.20865004178801 34.5 28.0 37.0 2.0 39.0 74-75 28.521234245768298 34.0 26.0 36.5 2.0 39.0 76-77 27.82998826282146 34.0 26.0 36.0 2.0 39.0 78-79 27.062052529582715 33.0 24.5 35.5 2.0 37.5 80-81 26.303138896035392 32.5 22.0 35.0 2.0 37.0 82-83 25.586826196895355 32.0 19.0 35.0 2.0 36.5 84-85 24.82382814700245 31.5 15.0 35.0 2.0 36.0 86-87 24.125440144195274 31.0 6.5 34.5 2.0 35.5 88-89 23.342565007375697 31.0 2.0 34.0 2.0 35.0 90-91 22.50138836664449 30.0 2.0 34.0 2.0 35.0 92-93 21.659766443848028 29.0 2.0 34.0 2.0 35.0 94-95 20.743762625839757 29.0 2.0 34.0 2.0 35.0 96-97 19.675816388511265 28.0 2.0 34.0 2.0 35.0 98-99 18.481837058733085 25.5 2.0 34.0 2.0 35.0 100-101 15.505116557337868 10.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4621499740483195 1101 2 0.4689718504805036 1101 3 0.41597072116034894 1101 4 0.2230869117673535 1101 5 0.3237881693352094 1101 6 0.08673686159719551 1101 7 0.18992544777165676 1101 8 0.21251606201568052 1101 9 0.25391650377869723 1101 10-11 0.3231519823073796 1101 12-13 0.28309563232797785 1101 14-15 0.6144367581974635 1101 16-17 0.5802013323905726 1101 18-19 0.4911813083620373 1101 20-21 0.4159328751673712 1101 22-23 0.45337013176686725 1101 24-25 0.49929766140402876 1101 26-27 0.38365332532411145 1101 28-29 0.6195436139139971 1101 30-31 0.3206054688223503 1101 32-33 0.5043260766906243 1101 34-35 0.6618146207267657 1101 36-37 0.6570831227526313 1101 38-39 0.7184360039422799 1101 40-41 0.7343908404928214 1101 42-43 0.9074137324072851 1101 44-45 0.8071548631144125 1101 46-47 1.0329592950825557 1101 48-49 0.6954885493364387 1101 50-51 0.8035133807502675 1101 52-53 0.8372464095209864 1101 54-55 0.865350822951612 1101 56-57 0.8794327886770432 1101 58-59 1.013369753707579 1101 60-61 1.125727445491215 1101 62-63 1.0418945617193245 1101 64-65 1.1519367883454983 1101 66-67 0.8513218816927299 1101 68-69 1.2728342846779945 1101 70-71 1.2408022571200483 1101 72-73 1.313916686598425 1101 74-75 1.5975438362158734 1101 76-77 1.9579136105269548 1101 78-79 2.326608519736425 1101 80-81 2.6281364770737703 1101 82-83 2.7561602530958673 1101 84-85 2.7576630116843504 1101 86-87 3.213260642853079 1101 88-89 3.499918006366787 1101 90-91 3.3886976975949885 1101 92-93 3.5856066368619466 1101 94-95 3.747427197700194 1101 96-97 4.40590991449243 1101 98-99 4.818956720938143 1101 100-101 4.86283176757958 1102 1 0.5175245668821944 1102 2 0.7295581696987448 1102 3 0.7661335876098576 1102 4 0.6286243710507406 1102 5 0.4833972898563843 1102 6 0.7056293697405209 1102 7 0.6638668158498291 1102 8 0.6929720880743133 1102 9 0.5405614549187661 1102 10-11 0.6399272266070568 1102 12-13 0.6959620818393759 1102 14-15 0.9385409927251445 1102 16-17 1.0207550783189063 1102 18-19 0.9594223507073139 1102 20-21 0.8719589337992915 1102 22-23 0.7253571024509071 1102 24-25 0.5945745343682063 1102 26-27 0.8698096771156472 1102 28-29 0.9517911709172537 1102 30-31 1.0233741984640474 1102 32-33 1.1834791711531594 1102 34-35 1.2041598975997303 1102 36-37 1.0894935462053965 1102 38-39 1.2738105967761513 1102 40-41 1.6220129903299636 1102 42-43 1.648456077684152 1102 44-45 1.8568291302153952 1102 46-47 2.1013632038773338 1102 48-49 2.2516774744178747 1102 50-51 2.148790253714445 1102 52-53 1.9707968981203408 1102 54-55 1.986686327837603 1102 56-57 2.2833415834653223 1102 58-59 2.0597866918183243 1102 60-61 2.093154155588941 1102 62-63 2.5329369069961984 1102 64-65 2.576203889322695 1102 66-67 2.5630482660575495 1102 68-69 2.780977607153563 1102 70-71 2.8621377620060393 1102 72-73 2.8766202696603145 1102 74-75 2.8784884616230393 1102 76-77 3.044232828767999 1102 78-79 3.0896378356973386 1102 80-81 3.454683708344131 1102 82-83 3.5835218166137857 1102 84-85 3.3798128488179024 1102 86-87 3.909514714514316 1102 88-89 4.258061328842359 1102 90-91 4.701401280656878 1102 92-93 4.554662011454781 1102 94-95 5.112248044605735 1102 96-97 4.844020663678098 1102 98-99 5.36944532028668 1102 100-101 5.5924734613906555 1103 1 0.4605678428244957 1103 2 0.4266627661518747 1103 3 0.33342586825719067 1103 4 0.2874010630521866 1103 5 0.038347912885178914 1103 6 0.21741262810358108 1103 7 0.16180384082268517 1103 8 0.3283011817095911 1103 9 0.3582182461911927 1103 10-11 0.38523770693382176 1103 12-13 0.40562549117705515 1103 14-15 0.6087209213741289 1103 16-17 0.6551095584388591 1103 18-19 0.934141858488708 1103 20-21 0.7263459872091431 1103 22-23 0.6712182264879232 1103 24-25 0.526447240019408 1103 26-27 0.6825753045392631 1103 28-29 0.5261280718013879 1103 30-31 0.6299483156244818 1103 32-33 0.6237614729989858 1103 34-35 0.7700535949266509 1103 36-37 0.7827875847763082 1103 38-39 0.7382203997853125 1103 40-41 0.7115145672908199 1103 42-43 0.7270119951651495 1103 44-45 0.7202798307996474 1103 46-47 0.9232752042909667 1103 48-49 0.7274672474389092 1103 50-51 1.0409752493196365 1103 52-53 0.9081682853288555 1103 54-55 1.290080671459961 1103 56-57 1.374867357727247 1103 58-59 1.394728008192864 1103 60-61 1.5076234177780705 1103 62-63 1.5929467306868048 1103 64-65 1.6908282624161295 1103 66-67 1.6500732389131372 1103 68-69 1.8635483118741014 1103 70-71 2.108815803472357 1103 72-73 2.1227373266894247 1103 74-75 2.378734053888177 1103 76-77 2.6401921897110725 1103 78-79 2.4697319363126127 1103 80-81 2.9779348328981854 1103 82-83 3.1358458949964962 1103 84-85 3.515085068454944 1103 86-87 3.732310847082438 1103 88-89 4.078277450035678 1103 90-91 4.093684513168121 1103 92-93 4.424785066105521 1103 94-95 4.871650350587842 1103 96-97 5.297277992668331 1103 98-99 5.605596501714732 1103 100-101 5.682952237048584 1104 1 0.38281680366457493 1104 2 0.3215240899565366 1104 3 0.4899440140555811 1104 4 0.34265033989411364 1104 5 0.15282079749589172 1104 6 0.21084708202808145 1104 7 0.22898097201206724 1104 8 0.2732779677277293 1104 9 0.3834813784987361 1104 10-11 0.32865028380734884 1104 12-13 0.47586540950273815 1104 14-15 0.7226930645958944 1104 16-17 0.7383651067020693 1104 18-19 0.8111028930301458 1104 20-21 0.8050839244463148 1104 22-23 0.6668586163855892 1104 24-25 0.805582610349262 1104 26-27 0.8805917852509921 1104 28-29 0.5638659847307252 1104 30-31 0.8032388671692487 1104 32-33 0.7691162458422696 1104 34-35 0.769675608101501 1104 36-37 0.655748765164752 1104 38-39 0.8924025626964109 1104 40-41 0.8297612431944614 1104 42-43 0.972596477043659 1104 44-45 1.1155373723052762 1104 46-47 1.1385356627367216 1104 48-49 1.2434131868151752 1104 50-51 0.9873703974758854 1104 52-53 1.1796612725186293 1104 54-55 1.343969924966359 1104 56-57 1.4095655759629935 1104 58-59 1.4415351741424516 1104 60-61 1.5808082302431288 1104 62-63 1.6518057209393646 1104 64-65 1.558288579876443 1104 66-67 1.513728164895781 1104 68-69 1.7843047716810965 1104 70-71 1.8241285237621092 1104 72-73 1.6606551175934356 1104 74-75 1.775170525362931 1104 76-77 2.1434117623793405 1104 78-79 2.4637680091388816 1104 80-81 2.6765711633875036 1104 82-83 2.8731085897630173 1104 84-85 3.1199198967200914 1104 86-87 3.44771350635952 1104 88-89 3.609392693478643 1104 90-91 3.871089165687632 1104 92-93 4.108875588480885 1104 94-95 4.483468819680432 1104 96-97 4.691841545501408 1104 98-99 5.039835301880025 1104 100-101 5.052424027806746 1105 1 0.4646125932547882 1105 2 0.5869371972094406 1105 3 0.6213394272550872 1105 4 0.3556072210173866 1105 5 0.4512949390852228 1105 6 0.5408301075451547 1105 7 0.426763680180116 1105 8 0.4537840656262091 1105 9 0.5181771293428454 1105 10-11 0.7067834135698945 1105 12-13 0.5960371377633962 1105 14-15 0.8091799001463613 1105 16-17 0.9325741788251705 1105 18-19 0.8612168250215078 1105 20-21 0.7446238606074971 1105 22-23 0.7419822349025296 1105 24-25 0.879306295288103 1105 26-27 0.8272755340286437 1105 28-29 1.0712012215432125 1105 30-31 0.9919439170779185 1105 32-33 0.9156791436817144 1105 34-35 1.1462392900882534 1105 36-37 0.985837881200105 1105 38-39 1.2387869517072758 1105 40-41 1.1239237277421523 1105 42-43 1.281128461028608 1105 44-45 1.2339655259612456 1105 46-47 0.8903530394410879 1105 48-49 0.9704630401335024 1105 50-51 1.001089515909804 1105 52-53 0.9596145465642678 1105 54-55 1.1064078850763295 1105 56-57 1.088976173944502 1105 58-59 1.193884156596006 1105 60-61 1.1338903904305866 1105 62-63 1.407495401560773 1105 64-65 1.7284693027680085 1105 66-67 1.484761993933219 1105 68-69 1.3115665754044166 1105 70-71 1.5434591387181023 1105 72-73 1.7892414383742619 1105 74-75 1.7831134763398886 1105 76-77 2.0669850755400994 1105 78-79 2.309608107664687 1105 80-81 2.5817931232022566 1105 82-83 2.795137824619747 1105 84-85 2.8793492681107082 1105 86-87 3.4449136392032997 1105 88-89 3.5179265225160776 1105 90-91 3.9363336812690974 1105 92-93 4.559356685918104 1105 94-95 4.400009023374224 1105 96-97 4.87212739025869 1105 98-99 5.162627004678967 1105 100-101 4.9988252529216 1106 1 0.16655862623463236 1106 2 0.21616609436487977 1106 3 0.14858203825206928 1106 4 0.21330992121911407 1106 5 0.04147131278277527 1106 6 -0.02001696739053216 1106 7 0.14859339535417604 1106 8 0.17151674750580526 1106 9 0.20363555289881674 1106 10-11 0.06693253540284871 1106 12-13 -0.03538833370773631 1106 14-15 0.33882844825585323 1106 16-17 0.13685559018490068 1106 18-19 0.45877063473039215 1106 20-21 0.2792901756372004 1106 22-23 0.17226789366662132 1106 24-25 0.24813182783302778 1106 26-27 0.36583183295707755 1106 28-29 0.141335445073274 1106 30-31 0.37674811081136994 1106 32-33 0.485896649650293 1106 34-35 0.36050418376434124 1106 36-37 0.4511183579457949 1106 38-39 0.3366843014750103 1106 40-41 0.15434610850945774 1106 42-43 -0.2531723366320833 1106 44-45 0.20619892962196928 1106 46-47 0.37371043214555044 1106 48-49 0.4637703708388088 1106 50-51 -0.2484512739778566 1106 52-53 0.24721078404877517 1106 54-55 0.16061318554086057 1106 56-57 0.21186172536678072 1106 58-59 -0.4497676395285879 1106 60-61 0.7233172221815565 1106 62-63 0.7861366948445081 1106 64-65 0.7142025583710669 1106 66-67 0.8176615645659808 1106 68-69 0.3077367399334996 1106 70-71 0.6908690548804479 1106 72-73 1.2647240138374016 1106 74-75 1.2906644174314401 1106 76-77 1.3760600852563876 1106 78-79 2.0483545681610487 1106 80-81 2.290223619380523 1106 82-83 2.6940896225950617 1106 84-85 2.836436834967998 1106 86-87 2.9487214973640548 1106 88-89 2.9179343880797113 1106 90-91 3.0857736068701165 1106 92-93 3.285563355916068 1106 94-95 3.4933605400399657 1106 96-97 3.7766174703949 1106 98-99 3.7203174438550306 1106 100-101 3.4842323189738256 1107 1 0.46628212853877926 1107 2 0.3741230836121936 1107 3 0.4546957095270301 1107 4 0.46009947575060295 1107 5 0.5785066430439088 1107 6 0.5328094535004055 1107 7 0.40370860272744835 1107 8 0.40941229282070424 1107 9 0.3804404684134113 1107 10-11 0.5292059609020754 1107 12-13 0.4839588245103883 1107 14-15 0.6299113975646051 1107 16-17 0.6547127330420395 1107 18-19 0.6938243981712517 1107 20-21 0.5145205903837464 1107 22-23 0.6654245756942672 1107 24-25 0.6543837479559187 1107 26-27 0.7385276854916398 1107 28-29 0.873270928944244 1107 30-31 0.7302657759419446 1107 32-33 0.7485233777608826 1107 34-35 0.7621170869901448 1107 36-37 0.6372320292207618 1107 38-39 0.9394108759757884 1107 40-41 0.6866732974495449 1107 42-43 0.4910596142127659 1107 44-45 0.3209147514345645 1107 46-47 0.10343393444969706 1107 48-49 0.23357835855002662 1107 50-51 0.2780387413831207 1107 52-53 0.41872384088441095 1107 54-55 0.4051600365393284 1107 56-57 0.43288323074312274 1107 58-59 0.7141724526373068 1107 60-61 0.5998456400002894 1107 62-63 0.8106451433852158 1107 64-65 0.6718600084478688 1107 66-67 0.8484065722464749 1107 68-69 1.1521991055249003 1107 70-71 1.3615142161707716 1107 72-73 1.580515104467203 1107 74-75 1.624051514205636 1107 76-77 1.6613826659015452 1107 78-79 1.5137001902808649 1107 80-81 1.9991253090886651 1107 82-83 2.228068117218715 1107 84-85 2.401513639883511 1107 86-87 2.6953267200983078 1107 88-89 2.900499672257901 1107 90-91 3.1577321322157417 1107 92-93 3.5555787168991735 1107 94-95 3.5294281283656197 1107 96-97 3.9301351355254717 1107 98-99 4.51789808901632 1107 100-101 4.829539538635887 1108 1 0.20926822565745695 1108 2 0.2629423461550111 1108 3 0.2788665739235654 1108 4 0.2617154338404646 1108 5 0.21019513790589173 1108 6 0.28727852462674264 1108 7 0.24210473409960542 1108 8 0.0841433022907836 1108 9 0.2695599525963672 1108 10-11 0.34570404673726074 1108 12-13 0.35264006569515516 1108 14-15 0.0916411980884817 1108 16-17 0.5766097711238487 1108 18-19 0.466691615580082 1108 20-21 0.46117483177000906 1108 22-23 0.3874505680376146 1108 24-25 0.3721733913167924 1108 26-27 0.31859518498790607 1108 28-29 0.43041983081810997 1108 30-31 0.481762854321957 1108 32-33 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0.47726769073255326 2311 60-61 0.3669708604588493 2311 62-63 0.5570462799715372 2311 64-65 0.4759840531274726 2311 66-67 0.5559717299968163 2311 68-69 0.5412868970200968 2311 70-71 0.8201130795844485 2311 72-73 0.6085887866149591 2311 74-75 0.6739525528103769 2311 76-77 0.9517050252979793 2311 78-79 1.2833807703318172 2311 80-81 1.171036929703984 2311 82-83 1.6763612781044692 2311 84-85 1.6635467576573717 2311 86-87 1.798281844534916 2311 88-89 1.6866156824479859 2311 90-91 2.2875998570834675 2311 92-93 2.255100566791395 2311 94-95 2.485936064873556 2311 96-97 3.0064000777163393 2311 98-99 3.1588102975211214 2311 100-101 3.111308106145197 2312 1 -0.24606694498184112 2312 2 -0.30076633659103535 2312 3 -0.13592393582064943 2312 4 -0.108647286552646 2312 5 -0.07642262573472891 2312 6 0.0216697842435849 2312 7 -0.11044162640185817 2312 8 -0.04905154459710559 2312 9 -0.21129095218829974 2312 10-11 -0.17678327998255838 2312 12-13 -0.16369110575460155 2312 14-15 -2.250838061783611E-4 2312 16-17 0.07259976233074639 2312 18-19 0.07719160279368964 2312 20-21 -0.04312336259493321 2312 22-23 -0.09560451376577817 2312 24-25 -0.08170511436725292 2312 26-27 0.06675542010012947 2312 28-29 0.06059708953399934 2312 30-31 0.04613347867157103 2312 32-33 0.025086438131474154 2312 34-35 -0.042933719943505366 2312 36-37 -0.07666316902590609 2312 38-39 0.18490300588214126 2312 40-41 0.1739008328151641 2312 42-43 0.2176675042306968 2312 44-45 -0.015789771378742046 2312 46-47 0.17666565391572675 2312 48-49 0.2343743338439097 2312 50-51 0.20234537617093196 2312 52-53 0.2044988956762026 2312 54-55 0.0764491379539507 2312 56-57 0.08329865214181353 2312 58-59 -0.065968013591025 2312 60-61 -0.18383238351036368 2312 62-63 -0.08931401182498533 2312 64-65 0.01853962590154623 2312 66-67 0.13570782747660104 2312 68-69 -0.04294036448904848 2312 70-71 0.12058773679240886 2312 72-73 0.11956272351482511 2312 74-75 0.3535962442315359 2312 76-77 0.48716369797964987 2312 78-79 0.47198670153808564 2312 80-81 0.428861312276549 2312 82-83 0.8184685954016224 2312 84-85 1.095539342214657 2312 86-87 1.1499471624174973 2312 88-89 1.448945577816776 2312 90-91 1.5017638118331966 2312 92-93 1.5841063120376475 2312 94-95 2.0851922247991723 2312 96-97 2.1736378253043114 2312 98-99 2.7158409063106 2312 100-101 2.6124933142884057 2313 1 -0.258113475856824 2313 2 -0.29378900429989585 2313 3 -0.2538757190443981 2313 4 -0.08039502974390444 2313 5 -0.17022962069235348 2313 6 -0.11817955748860243 2313 7 -0.19277491375606814 2313 8 -0.1817415533331399 2313 9 -0.44758150960856824 2313 10-11 -0.12095887426275453 2313 12-13 -0.10073897768741347 2313 14-15 -0.15217651452330472 2313 16-17 -0.3054521021227927 2313 18-19 -0.18606571171886088 2313 20-21 -0.209765123901974 2313 22-23 -0.10278971002001214 2313 24-25 -0.10407779050562027 2313 26-27 -0.10487890791495857 2313 28-29 -0.15381698314366332 2313 30-31 -0.21948697131080763 2313 32-33 -0.12600958927207984 2313 34-35 -0.15697631960326675 2313 36-37 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2314 1 -0.6057019984453476 2314 2 -0.6566705671814574 2314 3 -0.6397884174570976 2314 4 -0.3987100480589234 2314 5 -0.4112552617179901 2314 6 -0.5956826099916555 2314 7 -0.7548628258439791 2314 8 -0.6576115167031062 2314 9 -0.7905912776183328 2314 10-11 -0.7273614994153803 2314 12-13 -0.6888189532673934 2314 14-15 -0.7328465389433276 2314 16-17 -0.6765372669579577 2314 18-19 -0.4873859192890606 2314 20-21 -0.7046063937432336 2314 22-23 -0.7077500274803299 2314 24-25 -0.542798791726625 2314 26-27 -0.4490715208575651 2314 28-29 -0.7718878012144827 2314 30-31 -0.5615199383437712 2314 32-33 -0.48943297144545994 2314 34-35 -0.8797479923749378 2314 36-37 -0.7120536850649586 2314 38-39 -0.8192200764051663 2314 40-41 -0.4726124168361707 2314 42-43 -0.48757133816818055 2314 44-45 -0.6739662009463885 2314 46-47 -0.2664073353915697 2314 48-49 -0.06062799065632163 2314 50-51 -0.12836998877561 2314 52-53 -0.282704730544161 2314 54-55 -0.16716139203209934 2314 56-57 -0.10033105497115713 2314 58-59 0.083517690732549 2314 60-61 -0.013507534602887006 2314 62-63 0.02846260370267828 2314 64-65 -0.01810030901244275 2314 66-67 0.31743435002425 2314 68-69 0.7613657721915637 2314 70-71 0.7396689780755317 2314 72-73 0.8188682790703794 2314 74-75 1.0374642126183353 2314 76-77 1.366382665901547 2314 78-79 1.356537491868167 2314 80-81 1.3417245154378712 2314 82-83 1.3083657362663352 2314 84-85 1.7457398303597031 2314 86-87 2.143917989939581 2314 88-89 2.2575433230515536 2314 90-91 2.3413035607871713 2314 92-93 2.718535066105524 2314 94-95 3.1630193982068917 2314 96-97 2.854222437112771 2314 98-99 2.879703644571876 2314 100-101 2.955075252921601 2315 1 -0.5551887560068565 2315 2 -0.8950266242258067 2315 3 -0.6195819451173357 2315 4 -0.7848289949324538 2315 5 -0.5133591639276815 2315 6 -0.4503801495622568 2315 7 -0.6704481572967325 2315 8 -0.6112022548319231 2315 9 -0.654276436684313 2315 10-11 -0.7050716937422834 2315 12-13 -0.580364165616551 2315 14-15 -0.7997551347747063 2315 16-17 -0.8620362091300322 2315 18-19 -0.9282308103719643 2315 20-21 -0.8773301047603184 2315 22-23 -0.9129725631330459 2315 24-25 -0.8965149020541503 2315 26-27 -1.0565811196665322 2315 28-29 -1.3305110577573487 2315 30-31 -1.3059663417288263 2315 32-33 -1.2724538537523102 2315 34-35 -1.3115984076703455 2315 36-37 -1.167942260523354 2315 38-39 -0.9889338751827879 2315 40-41 -0.9368578329151518 2315 42-43 -1.0234346432927666 2315 44-45 -1.1652508741950953 2315 46-47 -0.84985036939878 2315 48-49 -1.0762916797332736 2315 50-51 -1.0499251917218544 2315 52-53 -0.6077752524059363 2315 54-55 -0.9060630140349275 2315 56-57 -0.951159686847042 2315 58-59 -0.6350971423657157 2315 60-61 -0.5470534129918505 2315 62-63 -0.516861405073378 2315 64-65 -0.4981289095452759 2315 66-67 -0.5308388438325089 2315 68-69 -0.2497120369292638 2315 70-71 -0.486092070349482 2315 72-73 -0.34353083548846897 2315 74-75 -0.49199825407967523 2315 76-77 -0.2553380675258161 2315 78-79 0.14029297690186127 2315 80-81 0.20737938928209232 2315 82-83 0.3444170996889966 2315 84-85 0.6591713066173455 2315 86-87 0.49866744143621133 2315 88-89 0.41320368192947043 2315 90-91 0.834745027641894 2315 92-93 0.8928464984828928 2315 94-95 1.109976183977146 2315 96-97 1.0630748897064102 2315 98-99 1.348257750511383 2315 100-101 2.0700523333165233 2316 1 -1.006028795830968 2316 2 -0.9006248155474701 2316 3 -1.0055563913133057 2316 4 -0.7511610284510795 2316 5 -0.8588042813258383 2316 6 -0.6281989498609377 2316 7 -0.5622157670204544 2316 8 -0.6357977912129087 2316 9 -0.9399376828470878 2316 10-11 -0.9822226105264917 2316 12-13 -0.7617764696072626 2316 14-15 -1.4051096108387569 2316 16-17 -1.0033755022520765 2316 18-19 -1.092982324517827 2316 20-21 -0.9725802499523866 2316 22-23 -0.8387140797679109 2316 24-25 -1.2949229747331543 2316 26-27 -0.8937202136680256 2316 28-29 -1.040678650887692 2316 30-31 -0.879166997167296 2316 32-33 -0.9296698995500421 2316 34-35 -1.008261064270357 2316 36-37 -0.9010242733002514 2316 38-39 -1.176450468562031 2316 40-41 -1.2615830050714578 2316 42-43 -1.1602429067956308 2316 44-45 -0.8609760048679576 2316 46-47 -0.976292956306601 2316 48-49 -1.0754972717020763 2316 50-51 -1.0445873090370412 2316 52-53 -0.907704730544161 2316 54-55 -1.0491466861497543 2316 56-57 -1.0893016432064542 2316 58-59 -0.8658695641694116 2316 60-61 -1.0286627633610514 2316 62-63 -0.5016027557744493 2316 64-65 -0.4358290671823788 2316 66-67 -0.333505192459409 2316 68-69 -0.6236832474162775 2316 70-71 -0.6469731787872135 2316 72-73 -0.2821529627596888 2316 74-75 0.13337924529806955 2316 76-77 0.10608854825448688 2316 78-79 0.08378422389430895 2316 80-81 0.22387320824832457 2316 82-83 0.4599588735212379 2316 84-85 0.803950614673429 2316 86-87 1.1946124343840232 2316 88-89 0.7827475714175627 2316 90-91 1.2479212078459945 2316 92-93 1.263919053033625 2316 94-95 1.6578314897101585 2316 96-97 1.4766897573742135 2316 98-99 1.5590745596045537 2316 100-101 1.5400017235098336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4882.0 3 926.0 4 732.0 5 695.0 6 738.0 7 858.0 8 1048.0 9 1298.0 10 1629.0 11 2294.0 12 3320.0 13 4491.0 14 5513.0 15 7509.0 16 9201.0 17 11279.0 18 9376.0 19 6782.0 20 7047.0 21 7526.0 22 8260.0 23 9370.0 24 10505.0 25 12031.0 26 13742.0 27 15867.0 28 18404.0 29 21220.0 30 24515.0 31 28878.0 32 33795.0 33 40080.0 34 48564.0 35 61525.0 36 71969.0 37 87255.0 38 58807.0 39 4956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.498539402537 25.434552559366836 16.627447626929968 19.439460411166205 2 14.337209036708773 24.262359991167358 40.992606467627596 20.407824504496272 3 16.196705832184765 30.583370772827305 33.49314286149418 19.726780533493752 4 10.769821228948178 21.22270383220072 42.378090570428604 25.629384368422496 5 9.919771148992302 41.221513578889166 36.89247627940417 11.966238992714361 6 23.818982193721343 43.48357222281458 19.283179235655208 13.414266347808868 7 22.90188625804595 37.083464672444066 24.967526880737996 15.047122188771992 8 23.548563449001563 36.0371994146285 22.352084814909006 18.062152321460932 9 25.129257895963192 18.248245214602374 24.8898547590511 31.732642130383333 10-11 21.41954337176385 29.92524551419787 30.1837416226703 18.47146949136798 12-13 23.048170607279804 29.66637529581248 29.57622292002327 17.709231176884444 14-15 21.32919044689573 30.597997892518443 28.79247702175092 19.280334638834915 16-17 17.344893296454735 33.27859404537042 30.35304937422868 19.023463283946164 18-19 17.140372929954005 31.301843940744778 34.48897815426245 17.068804975038773 20-21 17.831568783922904 30.67727020754269 34.42896825638558 17.06219275214883 22-23 18.390478155629953 30.16197399187884 34.11245516267829 17.335092689812914 24-25 17.887061152996726 31.66697128931536 33.96458761708933 16.481379940598583 26-27 17.40206138463823 31.045655824154068 34.64070454121437 16.911578249993337 28-29 16.848765890374974 30.52726158250812 35.37986514834824 17.244107378768668 30-31 17.679828094803707 30.419742294495776 34.78186710851135 17.11856250218917 32-33 17.438392513829086 31.03669609511518 34.44620837473493 17.078703016320798 34-35 17.894207063116074 30.86469949549171 34.34722292017865 16.89387052121356 36-37 18.16524728464669 30.17569373327293 33.74705432229426 17.91200465978612 38-39 17.039858945195093 31.594966852729893 33.85193508799176 17.51323911408325 40-41 16.954147465964034 30.877394393915054 34.829776397619554 17.338681742501354 42-43 17.212008353133832 30.610596420759805 34.09010452075684 18.087290705349524 44-45 17.30404134980627 30.437324442211516 33.959289623725894 18.299344584256318 46-47 18.259828924489867 30.971808168870833 32.933101497291354 17.835261409347947 48-49 17.544551470661006 30.83961318307878 32.96629930275207 18.64953604350815 50-51 16.885362953625428 30.850639494433256 33.117681895479464 19.146315656461855 52-53 17.488433555553186 31.58214040498921 32.65094229033107 18.278483749126522 54-55 17.88606622704087 30.978470616803165 32.39447877091528 18.74098438524069 56-57 17.84182617869915 31.174306954283114 32.14576606119842 18.838100805819316 58-59 17.730336650998154 30.1525110853141 32.96089215369098 19.156260109996765 60-61 18.060023475929558 31.119004084689948 32.23449134724736 18.586481092133127 62-63 17.604158578933987 30.586168416661717 32.92616832531792 18.88350467908638 64-65 17.78744705663836 30.451453487486944 32.55343054713977 19.20766890873493 66-67 17.671166038307458 30.572358740967577 31.708885859367804 20.047589361357165 68-69 17.647587199632177 30.101067154050337 32.34917761617927 19.902168030138213 70-71 18.207486771056878 30.45309939045232 32.30823456509112 19.031179273399683 72-73 18.189880731703045 29.638403980532786 32.51166837825719 19.660046909506978 74-75 17.605288559622664 30.89520016321211 31.672665320775746 19.82684595638948 76-77 17.849212384738998 30.44352030108499 32.28838144740338 19.41888586677263 78-79 18.045389220098286 29.995569778275776 31.954077935364435 20.004963066261503 80-81 18.224176346151943 30.18233387049751 31.429204324615647 20.164285458734906 82-83 18.493667918162522 30.240600839811577 31.50785458288039 19.757876659145513 84-85 18.126500030828417 29.884382019649504 31.54021053144384 20.448907418078242 86-87 18.13047333787502 30.05720244642266 31.693504496652725 20.118819719049593 88-89 18.971066968350595 29.700133077937384 31.342747252412607 19.986052701299414 90-91 18.888981947479426 29.520249126306737 31.605692140186846 19.985076786026998 92-93 19.528894816655402 29.621754700089525 31.234964890163763 19.61438559309131 94-95 19.26902635059053 28.8923098240477 31.526607541334815 20.312056284026948 96-97 19.970918171194523 28.612865283376536 30.95577963282288 20.46043691260606 98-99 19.680633617057232 28.81499024437405 30.535299531592923 20.969076606975786 100-101 20.073484645869385 28.478805348743467 31.21649701344806 20.231212991939092 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2482.0 1 1529.0 2 697.0 3 816.0 4 697.0 5 658.0 6 1047.0 7 2133.5 8 3196.0 9 3361.5 10 2592.5 11 1796.5 12 1604.0 13 1572.5 14 1596.0 15 1589.0 16 1594.0 17 1667.0 18 1784.5 19 1943.0 20 2084.0 21 2279.5 22 2557.0 23 2817.5 24 3149.0 25 3869.0 26 5581.5 27 7518.0 28 8141.5 29 9113.0 30 11844.0 31 14489.0 32 17777.0 33 22914.0 34 28916.0 35 33127.0 36 37842.5 37 43131.0 38 42369.0 39 39570.5 40 36837.0 41 33135.0 42 30099.0 43 27700.0 44 25886.0 45 24118.0 46 21050.0 47 17483.5 48 15710.0 49 13340.0 50 9538.0 51 6047.5 52 3933.5 53 2820.0 54 2141.5 55 1728.0 56 1502.0 57 1376.5 58 1279.5 59 1248.5 60 1258.5 61 1280.0 62 1168.0 63 832.5 64 513.0 65 409.0 66 346.5 67 306.5 68 257.0 69 194.5 70 170.5 71 164.5 72 144.5 73 126.5 74 104.0 75 91.0 76 83.0 77 61.5 78 46.0 79 44.5 80 39.0 81 23.5 82 14.0 83 10.5 84 6.0 85 3.0 86 3.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30720656673065533 2 0.03531809885109615 3 0.041407426239216186 4 0.0446043231179792 5 0.05937094203417026 6 0.05739191063303125 7 0.028924305093570125 8 0.03196896878763014 9 0.03866722891456217 10-11 0.03105556967941214 12-13 0.03364353381936315 14-15 0.027249740061837117 16-17 0.020932062896662593 18-19 0.02565129162245561 20-21 0.03821052936045317 22-23 0.03158838582587264 24-25 0.05130258324491122 26-27 0.04407150697151869 28-29 0.036459847736368664 30-31 0.03744936343693816 32-33 0.03189285219527864 34-35 0.03166450241822414 36-37 0.031131686271763638 38-39 0.01842021534906308 40-41 0.01803963238730558 42-43 0.019409731049632586 44-45 0.007916125604556035 46-47 0.015375551655003067 48-49 0.013777103215621561 50-51 0.006089327388120027 52-53 0.00456699554109002 54-55 0.006165443980471527 56-57 0.006089327388120027 58-59 0.007535542642798533 60-61 0.006698260126932029 62-63 0.004262529171684019 64-65 0.008068358789259034 66-67 0.0026640807323025117 68-69 0.007078843088689531 70-71 0.013472636846215559 72-73 0.013777103215621561 74-75 0.01111302248331905 76-77 0.009514574043937542 78-79 0.005328161464605023 80-81 0.006469910349877529 82-83 0.01111302248331905 84-85 0.0038819462099265174 86-87 0.0014462152546785064 88-89 0.01963808082668709 90-91 0.02922877146297613 92-93 0.013853219807973062 94-95 0.0223021615589896 96-97 0.01819186557200858 98-99 0.013168170476809559 100-101 0.10587817996093696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 656887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.96328116598157 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0964005250141 16.932899216160134 2 8.231408975663147 3.61577499449348 3 3.1686540011240703 2.087821162332013 4 1.7815863526176237 1.5651792793606578 5 1.1846812906184148 1.3009744138965083 6 0.9354581337043394 1.2327438005731672 7 0.6435414944369509 0.98939979490063 8 0.552210661930928 0.9702686424673413 9 0.47577338613457576 0.9404590185868323 >10 4.397101919478526 21.01456253964456 >50 0.8390555150274003 12.91618473249969 >100 0.6532151199634803 27.249986495316534 >500 0.03536514980694001 5.030651926128708 >1k 0.004160605859640002 2.135258999252959 >5k 0.0013868686198800005 2.017834984386765 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7285 1.10901875056136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5964 0.907918713568696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4637 0.7059052774678141 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4005 0.6096939047355177 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1605 0.24433426144831608 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.19714197419038584 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1249 0.19013924769404783 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.18709458399998782 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 967 0.14720948960780164 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 966 0.14705725642309864 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 947 0.14416482591374163 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 843 0.12833257470462955 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 810 0.12330887960943056 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 799 0.12163431457769752 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.12132984820829153 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 793 0.12072091546947952 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 785 0.11950304999185553 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 780 0.11874188406834052 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 778 0.11843741769893452 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.1152405208201715 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 750 0.1141748885272505 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 739 0.1125003234955175 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 720 0.10960789298616047 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 716 0.10899896024734847 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 696 0.10595429655328847 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 660 0.10047390190398044 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5223318470300066E-4 0.0 10-11 0.0 0.0 0.0 1.5223318470300066E-4 0.0 12-13 0.0 0.0 0.0 1.5223318470300066E-4 0.0 14-15 0.0 0.0 0.0 1.5223318470300066E-4 0.0 16-17 0.0 0.0 0.0 2.28349777054501E-4 0.0 18-19 0.0 0.0 0.0 5.328161464605023E-4 0.0 20-21 0.0 0.0 0.0 0.0012939820699755058 0.0 22-23 0.0 0.0 0.0 0.00441476235638702 0.0 24-25 0.0 0.0 0.0 0.012483121145646054 0.0 26-27 0.0 0.0 0.0 0.02527070866069811 0.0 28-29 0.0 0.0 0.0 0.050693650506099225 0.0 30-31 0.0 0.0 0.0 0.11645838629779551 0.0 32-33 0.0 0.0 0.0 0.21327869176890396 0.0 34-35 0.0 0.0 0.0 0.36406566121722606 0.0 36-37 0.0 0.0 0.0 0.5704938596744951 0.0 38-39 0.0 0.0 0.0 0.8303559059625172 0.0 40-41 0.0 0.0 0.0 1.0925775666134356 0.0 42-43 0.0 0.0 0.0 1.3537335949714335 0.0 44-45 0.0 0.0 0.0 1.6659638567972879 0.0 46-47 0.0 0.0 0.0 2.039772441835506 0.0 48-49 0.0 0.0 0.0 2.406882766746792 0.0 50-51 0.0 0.0 0.0 2.7870851455425365 0.0 52-53 0.0 0.0 0.0 3.1376020533211957 0.0 54-55 0.0 0.0 0.0 3.5296786205237733 0.0 56-57 0.0 0.0 0.0 4.017205394535133 0.0 58-59 0.0 0.0 0.0 4.508309648387014 0.0 60-61 0.0 0.0 0.0 5.020726548097313 0.0 62-63 0.0 0.0 0.0 5.510460703286867 0.0 64-65 0.0 0.0 0.0 6.050812392390167 0.0 66-67 0.0 0.0 0.0 6.637823552604939 0.0 68-69 0.0 0.0 0.0 7.2688301031988765 0.0 70-71 0.0 0.0 0.0 7.986000636334712 0.0 72-73 0.0 0.0 0.0 8.652629752149153 0.0 74-75 0.0 0.0 0.0 9.389057783149918 0.0 76-77 0.0 0.0 0.0 10.137892818703978 0.0 78-79 0.0 1.5223318470300066E-4 0.0 10.966193576673005 0.0 80-81 0.0 1.5223318470300066E-4 0.0 11.842523904415827 0.0 82-83 0.0 1.5223318470300066E-4 0.0 12.668693397799013 0.0 84-85 0.0 1.5223318470300066E-4 0.0 13.49653745621393 0.0 86-87 0.0 1.5223318470300066E-4 0.0 14.368529138192718 0.0 88-89 0.0 1.5223318470300066E-4 0.0 15.277513484054335 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGTCG 15 6.614228E-4 95.00876 8 GTGTCGC 20 0.002072921 71.25657 9 GTATGGT 130 1.9645086E-10 36.673115 1 GGTATCA 3960 0.0 35.03394 1 CATGGAT 55 0.0025690028 34.57234 4 GATCCTC 70 2.0430179E-4 33.954975 5 GGATATG 325 0.0 32.272343 1 CATTCCG 265 0.0 32.267124 9 TACAGGG 60 0.0039347145 31.69131 2 GTGCACC 90 2.409879E-5 31.669588 8 GTATCAA 6050 0.0 30.969082 1 CTTTAGG 300 0.0 30.106745 2 AGGGGTC 240 0.0 29.710606 5 CCTTTAG 305 0.0 29.69921 1 GGGTCCT 240 0.0 29.690235 7 GGCATTC 305 0.0 29.592892 7 GTAAGGT 150 3.3069227E-8 28.522179 4 GGTCCTA 250 0.0 28.502626 8 GTTCGCA 120 5.373211E-6 27.710886 9 GATTGGC 345 0.0 27.538773 9 >>END_MODULE