SRR938345_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR938345_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656887
Sequences flagged as poor quality	0
Sequence length	101
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6873	1.0462986784637236	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5770	0.8783854757363139	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4421	0.673022909571966	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4267	0.6495789991277039	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	1971	0.3000516070496143	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1537	0.23398240488851202	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	1294	0.19698974100568287	TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.19379284412691986	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1198	0.1823753552741948	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1065	0.1621283417086957	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.1615194089698837	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1010	0.15375551655003067	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	991	0.15086308604067367	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	862	0.13122500521398658	No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG	860	0.13092053884458058	No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC	856	0.13031160610576858	No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA	855	0.13015937292106558	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	833	0.12681024285759956	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	830	0.12635354330349055	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	778	0.11843741769893452	No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA	777	0.11828518451423152	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	775	0.11798071814482552	No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC	756	0.1150882876354685	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	755	0.1149360544507655	RNA PCR Primer, Index 11 (95% over 23bp)
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT	753	0.1146315880813595	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	720	0.10960789298616047	No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT	718	0.10930342661675448	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA	704	0.10717216203091247	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	698	0.10625876292269447	No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG	695	0.10580206336858547	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	682	0.10382303196744647	No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG	659	0.10032166871927745	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAG	35	3.37669E-6	67.987465	1
ACGCAGT	30	1.3017048E-4	63.32455	7
TGGGCGC	30	1.3017048E-4	63.32455	6
GTATGGT	80	1.8189894E-12	59.489033	1
CGTCAGA	50	2.7937936E-5	47.591225	2
GTGGTAC	145	0.0	39.385845	1
TGGTACT	160	1.8189894E-12	35.69342	2
CATTCCG	180	0.0	34.3008	9
GTCAGAT	70	2.0348336E-4	33.978184	3
ATGGGCG	100	1.2833298E-6	33.29862	5
TACCTGG	75	3.0425814E-4	31.727488	2
GACCGCG	75	3.0795255E-4	31.66228	7
GCCTATC	105	1.9020899E-6	31.66228	7
TACAATC	90	2.4136867E-5	31.662275	7
GCCACCG	55	2.7335518E-6	30.227686	28-29
GGTACTC	190	1.2732926E-11	30.043869	3
TAGGGTC	160	1.873559E-9	29.73091	4
TGTAGAT	130	2.994948E-7	29.273514	5
TACTCCC	215	1.8189894E-12	28.762926	5
ACGCACA	50	4.958325E-5	28.496048	88-89

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content