##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938345_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 656887 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.773184733447305 31.0 31.0 34.0 30.0 34.0 2 32.02406958883339 31.0 31.0 34.0 30.0 34.0 3 32.0465924885102 33.0 31.0 34.0 30.0 34.0 4 35.746142030516665 37.0 35.0 37.0 33.0 37.0 5 35.63235076961487 37.0 35.0 37.0 33.0 37.0 6 35.68416181169668 37.0 35.0 37.0 33.0 37.0 7 35.62859974394378 37.0 35.0 37.0 33.0 37.0 8 35.637025850717094 37.0 35.0 37.0 33.0 37.0 9 37.155646252704045 39.0 37.0 39.0 33.0 39.0 10-11 37.11554270369181 39.0 37.0 39.0 33.0 39.0 12-13 37.04148810982711 39.0 37.0 39.0 33.0 39.0 14-15 38.010919686338745 40.0 37.0 41.0 33.0 41.0 16-17 37.943161456993366 40.0 37.0 41.0 33.0 41.0 18-19 38.06264928366675 40.0 37.0 41.0 33.0 41.0 20-21 38.10922426536071 40.0 37.0 41.0 33.0 41.0 22-23 38.100298833741576 40.0 37.0 41.0 33.0 41.0 24-25 38.00683450882724 40.0 37.0 41.0 33.0 41.0 26-27 37.82473545678329 40.0 37.0 41.0 33.0 41.0 28-29 37.69987303752396 40.0 37.0 41.0 32.5 41.0 30-31 37.396622250097806 39.0 36.0 41.0 32.0 41.0 32-33 37.09418971603944 39.0 35.5 41.0 31.5 41.0 34-35 36.87766541277267 39.0 35.0 41.0 31.0 41.0 36-37 36.776372496334986 39.0 35.0 41.0 31.0 41.0 38-39 36.59259887925929 39.0 35.0 41.0 31.0 41.0 40-41 36.437819594542134 39.0 35.0 41.0 30.0 41.0 42-43 36.14604186108113 39.0 35.0 40.5 30.0 41.0 44-45 35.76089570961216 39.0 35.0 40.0 29.5 41.0 46-47 35.26573368022202 38.0 35.0 40.0 26.5 41.0 48-49 34.78043560003471 38.0 34.5 40.0 24.0 41.0 50-51 34.44959711487668 38.0 34.0 40.0 23.5 41.0 52-53 34.05770246632983 38.0 33.0 40.0 22.5 41.0 54-55 33.78851918214244 38.0 33.0 40.0 21.0 41.0 56-57 33.47122107759782 38.0 33.0 40.0 19.0 41.0 58-59 33.05521725958955 37.5 32.5 40.0 15.5 41.0 60-61 32.690090533074944 37.0 32.0 40.0 11.0 41.0 62-63 32.25802230825089 36.5 31.0 39.0 8.0 40.5 64-65 31.80872509274807 36.0 31.0 39.0 4.5 40.0 66-67 31.30677803031572 35.5 30.5 39.0 2.0 40.0 68-69 30.753481192351195 35.0 29.5 38.0 2.0 40.0 70-71 30.24843618461014 35.0 29.0 38.0 2.0 40.0 72-73 29.70442785441027 34.0 28.5 37.0 2.0 39.0 74-75 29.09783874547677 34.0 28.0 36.5 2.0 39.0 76-77 27.725009780982116 32.5 26.0 35.0 2.0 37.0 78-79 28.35426184412235 33.5 27.0 36.0 2.0 37.5 80-81 28.043165719522534 33.5 26.5 35.0 2.0 37.0 82-83 27.569957237698418 33.0 26.0 35.0 2.0 37.0 84-85 27.010241487500892 33.0 26.0 35.0 2.0 36.0 86-87 26.463405425895168 32.5 25.0 35.0 2.0 36.0 88-89 25.77695174360278 32.0 22.5 35.0 2.0 35.0 90-91 25.28587565289007 32.0 20.0 34.0 2.0 35.0 92-93 24.61413150206961 31.0 17.5 34.0 2.0 35.0 94-95 23.892849150614943 31.0 4.5 34.0 2.0 35.0 96-97 22.982690325733344 31.0 2.0 34.0 2.0 35.0 98-99 21.928129191169866 30.0 2.0 34.0 2.0 35.0 100-101 18.740745364118943 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34285487005958615 1101 2 0.28394395658074956 1101 3 0.32595222118122535 1101 4 0.3000131413016689 1101 5 0.293089786684952 1101 6 0.23339941854205648 1101 7 0.21638067757223212 1101 8 0.2186163092454123 1101 9 0.2521347794277773 1101 10-11 0.22749580605854192 1101 12-13 0.17272888252512786 1101 14-15 0.3911981361888692 1101 16-17 0.4295997968844034 1101 18-19 0.5587756684707514 1101 20-21 0.19531896762609335 1101 22-23 0.1878179664284474 1101 24-25 0.3475915976041293 1101 26-27 0.49260537201737975 1101 28-29 0.2662297397772946 1101 30-31 0.023594203431564154 1101 32-33 0.10224834586927045 1101 34-35 -0.037126007445607456 1101 36-37 0.2901844314531914 1101 38-39 0.24407586561009964 1101 40-41 0.20035488326107753 1101 42-43 0.2197204289522574 1101 44-45 0.5606713411610968 1101 46-47 0.6383047236124284 1101 48-49 0.6154581600543239 1101 50-51 0.688002733941623 1101 52-53 0.4900432559548662 1101 54-55 0.5966032490099522 1101 56-57 0.46683086325360534 1101 58-59 0.6325187604347704 1101 60-61 0.5343401110451254 1101 62-63 0.6471950029054341 1101 64-65 0.667021041131477 1101 66-67 0.8623849155669099 1101 68-69 0.7363302629155868 1101 70-71 0.8064477540161263 1101 72-73 0.8282681977391775 1101 74-75 0.7136774533799795 1101 76-77 0.589167704291139 1101 78-79 1.1498679892574017 1101 80-81 1.551920956688683 1101 82-83 1.6622883602761895 1101 84-85 1.5164376539276603 1101 86-87 1.5175471996701084 1101 88-89 1.5048483195203808 1101 90-91 1.6659677077096546 1101 92-93 2.2987896353755595 1101 94-95 2.3831578075708855 1101 96-97 2.77028495695588 1101 98-99 3.3409430098934187 1101 100-101 3.5057725464612943 1102 1 0.30213825768173663 1102 2 0.2904586145612029 1102 3 0.31618023421054176 1102 4 0.22998056801176858 1102 5 0.06833408635921501 1102 6 0.11450691039873107 1102 7 -0.018147009724181373 1102 8 0.295163540515766 1102 9 0.4866624667241908 1102 10-11 0.36918961713347187 1102 12-13 0.3535106414827922 1102 14-15 0.47263136094456826 1102 16-17 0.4825313929756163 1102 18-19 0.43173983785185754 1102 20-21 0.5479248308182747 1102 22-23 0.4589906048649297 1102 24-25 0.5023147246399589 1102 26-27 0.5235499974245457 1102 28-29 0.3110180133929319 1102 30-31 0.5024215649950818 1102 32-33 0.29524508854027687 1102 34-35 0.5239489111211739 1102 36-37 0.5035394803131226 1102 38-39 0.7758348232648231 1102 40-41 0.7834167725118917 1102 42-43 0.7343784094082793 1102 44-45 0.6250035887181014 1102 46-47 0.3809757333844175 1102 48-49 0.3784874760152306 1102 50-51 0.22139035609145452 1102 52-53 0.3670790865737601 1102 54-55 0.5363426626907284 1102 56-57 0.7705767915923687 1102 58-59 1.082844493333802 1102 60-61 1.0408547690255858 1102 62-63 1.123579367726279 1102 64-65 1.085587816375778 1102 66-67 0.8501699318535465 1102 68-69 1.045776516987246 1102 70-71 1.1484672979900665 1102 72-73 1.1523724322668656 1102 74-75 1.212863121132422 1102 76-77 1.3937279648774599 1102 78-79 1.8648517026124516 1102 80-81 1.9460577645062713 1102 82-83 1.9464903146735821 1102 84-85 2.1784897711915043 1102 86-87 2.177970652438834 1102 88-89 2.273577961214194 1102 90-91 2.1415377402829456 1102 92-93 2.5178450099683936 1102 94-95 2.8237115534992228 1102 96-97 3.2368973348060415 1102 98-99 3.3718876353005847 1102 100-101 4.330691113236536 1103 1 0.22676469892055806 1103 2 0.4061196455316818 1103 3 0.3291371650827841 1103 4 0.23523882766742332 1103 5 0.04745615864089814 1103 6 0.25614384747540697 1103 7 0.19340875264590807 1103 8 0.16954438948945239 1103 9 0.402111512792132 1103 10-11 0.2642622607403098 1103 12-13 0.40517808370398 1103 14-15 0.5412575953688403 1103 16-17 0.5829501924172149 1103 18-19 0.6831823692618073 1103 20-21 0.67202906534596 1103 22-23 0.5040787595621978 1103 24-25 0.5210934847867961 1103 26-27 0.5383837702387737 1103 28-29 0.5838270718697345 1103 30-31 0.6668908271842113 1103 32-33 0.8336686447162762 1103 34-35 0.8248433840026124 1103 36-37 0.8945818255899951 1103 38-39 0.9794747591523176 1103 40-41 1.0432223668451712 1103 42-43 1.2751958438739237 1103 44-45 1.754391934718825 1103 46-47 1.6531384964266564 1103 48-49 1.8542177898562997 1103 50-51 2.0424001280784267 1103 52-53 2.290712818025078 1103 54-55 2.202996403448701 1103 56-57 2.273929772306907 1103 58-59 2.42975520115656 1103 60-61 2.3856766500038162 1103 62-63 2.599578540468123 1103 64-65 2.4608424567770015 1103 66-67 2.2583029136021686 1103 68-69 2.6664114376221733 1103 70-71 2.3529422475790227 1103 72-73 2.228379360553923 1103 74-75 2.6605106057505914 1103 76-77 2.4640552488855256 1103 78-79 2.7099708794905872 1103 80-81 2.7336540625260213 1103 82-83 2.983362761806614 1103 84-85 2.799915757438338 1103 86-87 2.8233018142195085 1103 88-89 3.0700181659605867 1103 90-91 3.363695332961708 1103 92-93 3.3822935907036147 1103 94-95 3.415800897380098 1103 96-97 3.8607740733407603 1103 98-99 4.400024753339988 1103 100-101 4.688684495171287 1104 1 0.27209151775426577 1104 2 0.26363927410797316 1104 3 0.1274696476419095 1104 4 0.22349085129463475 1104 5 0.04507885243510401 1104 6 0.11738014393375806 1104 7 0.14158931123994734 1104 8 0.2034270798805622 1104 9 0.34114017172890954 1104 10-11 0.2577744997349427 1104 12-13 0.2153472662315039 1104 14-15 0.27212854507593676 1104 16-17 0.5071571493194256 1104 18-19 0.4964836162537196 1104 20-21 0.4417074726966561 1104 22-23 0.3982436639108684 1104 24-25 0.353137287504417 1104 26-27 0.37993072082094415 1104 28-29 0.43610593134614106 1104 30-31 0.2718520561883153 1104 32-33 0.266394282189232 1104 34-35 0.2227407563257131 1104 36-37 0.25836436770331517 1104 38-39 0.42834566024373544 1104 40-41 0.46243892534197784 1104 42-43 0.6071181074194598 1104 44-45 0.40927537865280783 1104 46-47 0.1608416491527791 1104 48-49 -0.01683279474712407 1104 50-51 0.14240012807842817 1104 52-53 0.11685710933016225 1104 54-55 0.11446119355055373 1104 56-57 0.2150525511785375 1104 58-59 0.3561595862898841 1104 60-61 0.09026839992481683 1104 62-63 0.2644962584486308 1104 64-65 0.3572193740574363 1104 66-67 0.32785248943045886 1104 68-69 0.6097121984573839 1104 70-71 0.4098043492343848 1104 72-73 0.5494809916096095 1104 74-75 0.37147708524693357 1104 76-77 0.5351848339507903 1104 78-79 0.6542925693712363 1104 80-81 0.8218465319589221 1104 82-83 0.9193517260497899 1104 84-85 0.9869159275931771 1104 86-87 1.1010455610291068 1104 88-89 1.4109282512810886 1104 90-91 1.389710792744399 1104 92-93 1.7470277845342856 1104 94-95 1.7053764825912126 1104 96-97 2.1531158901083067 1104 98-99 2.858997990414295 1104 100-101 3.180078306396645 1105 1 0.3508706465204803 1105 2 0.3641900222767589 1105 3 0.3640099897146456 1105 4 0.29319924074831505 1105 5 0.13632592341427596 1105 6 0.3149552836964844 1105 7 0.24484306130913325 1105 8 0.32782513150512926 1105 9 0.4850288966131302 1105 10-11 0.35439182662288715 1105 12-13 0.3796330464119464 1105 14-15 0.5723797028463693 1105 16-17 0.5954071631573186 1105 18-19 0.5826660181430512 1105 20-21 0.5203724388399351 1105 22-23 0.4636288647448339 1105 24-25 0.3283247909638831 1105 26-27 0.6264551031896204 1105 28-29 0.5792301126041011 1105 30-31 0.686230088993355 1105 32-33 0.8710491189943426 1105 34-35 0.7692591422737891 1105 36-37 0.8123529099273483 1105 38-39 0.9214163349794333 1105 40-41 0.7941355433607598 1105 42-43 0.7929956143845374 1105 44-45 0.6541576643038312 1105 46-47 0.6937700703413654 1105 48-49 0.5508806316998616 1105 50-51 0.4865266341025247 1105 52-53 0.8081994311442173 1105 54-55 0.6559391267227497 1105 56-57 0.860808238552849 1105 58-59 1.14757409578268 1105 60-61 1.1400536819410831 1105 62-63 1.013637155882158 1105 64-65 1.035938077354544 1105 66-67 1.0378759677234122 1105 68-69 0.8876439868667632 1105 70-71 0.85290638986244 1105 72-73 1.0611843901963027 1105 74-75 1.1179737918282342 1105 76-77 0.9113587485986621 1105 78-79 0.9106210994179165 1105 80-81 1.2919993484315526 1105 82-83 1.5041411133078562 1105 84-85 1.4174693049617666 1105 86-87 1.7373580391191084 1105 88-89 1.9440284929829623 1105 90-91 2.082893554418966 1105 92-93 2.2942126956950126 1105 94-95 2.532560794109873 1105 96-97 3.158218613405783 1105 98-99 3.2930396701500833 1105 100-101 4.387147394387199 1106 1 0.0956331064986422 1106 2 0.2660786829100772 1106 3 0.03891699099163759 1106 4 0.05597615485635998 1106 5 -0.02197548498429569 1106 6 -0.044521795125717745 1106 7 0.21921520425881624 1106 8 0.04015370848586741 1106 9 0.1998073653266914 1106 10-11 0.3357932387228999 1106 12-13 0.09711356757494372 1106 14-15 0.17392528610104563 1106 16-17 0.08379504648787872 1106 18-19 0.10422691611281465 1106 20-21 0.3067448871585654 1106 22-23 0.2031827022145265 1106 24-25 0.053940386988536204 1106 26-27 -0.02572273257740676 1106 28-29 0.24450295512626496 1106 30-31 0.3768242629240959 1106 32-33 0.510217543845819 1106 34-35 0.564833143347208 1106 36-37 0.6869781389540535 1106 38-39 0.44373487179952065 1106 40-41 0.48282810161219913 1106 42-43 0.5556105904177002 1106 44-45 0.1838594206379227 1106 46-47 0.052165634163479524 1106 48-49 0.5037057570862018 1106 50-51 0.16160135695707112 1106 52-53 0.5642663254495517 1106 54-55 0.525705056802515 1106 56-57 0.34379664378668195 1106 58-59 0.6529758872804692 1106 60-61 0.4688307718676157 1106 62-63 0.743638960334998 1106 64-65 0.26312100260393834 1106 66-67 0.649188730294437 1106 68-69 0.5223510177552981 1106 70-71 0.8072433228478388 1106 72-73 1.091717814214956 1106 74-75 1.2745147020127519 1106 76-77 1.0866358940468182 1106 78-79 1.27097958404768 1106 80-81 1.404186576606925 1106 82-83 1.6464708007211044 1106 84-85 1.362383755390205 1106 86-87 2.0428102627602165 1106 88-89 2.2478983502923846 1106 90-91 2.394801323745117 1106 92-93 2.023153805757378 1106 94-95 2.1756319265659663 1106 96-97 2.3738565106167506 1106 98-99 3.0971061665504287 1106 100-101 3.519278453184601 1107 1 0.17073295288880885 1107 2 0.08961170902374249 1107 3 0.09707367301928826 1107 4 0.25523882766741934 1107 5 0.16983711102184884 1107 6 0.14074702207858536 1107 7 0.13293256216972082 1107 8 0.08176661171167154 1107 9 0.3935400842207031 1107 10-11 0.2976749591530137 1107 12-13 0.2497018932277868 1107 14-15 0.33943219854344875 1107 16-17 0.2158073352743557 1107 18-19 0.3046109406903881 1107 20-21 0.39345763677453505 1107 22-23 0.3084438389272819 1107 24-25 0.2781569768502905 1107 26-27 0.37370123055622884 1107 28-29 0.5328746909173603 1107 30-31 0.275541620835007 1107 32-33 0.20906547011310295 1107 34-35 0.06024020939943853 1107 36-37 0.24101039701856308 1107 38-39 0.3413001559777129 1107 40-41 0.3879049065277158 1107 42-43 0.3478148914929733 1107 44-45 0.17002685535374695 1107 46-47 0.13559881388697903 1107 48-49 0.31929715493566135 1107 50-51 0.3674001280784225 1107 52-53 0.284442976755237 1107 54-55 0.34672656217886555 1107 56-57 0.29099326437040673 1107 58-59 0.28316789956925703 1107 60-61 0.37464490397206873 1107 62-63 0.22354679443638048 1107 64-65 0.40909642503096677 1107 66-67 0.6507632310624878 1107 68-69 0.7407765169872462 1107 70-71 0.39484700948378304 1107 72-73 0.7999666621412267 1107 74-75 0.9184471136871011 1107 76-77 1.140007629837907 1107 78-79 0.9783835779032835 1107 80-81 1.2787334276053883 1107 82-83 1.3585214919653446 1107 84-85 1.3535665510891306 1107 86-87 1.8244922904099852 1107 88-89 1.8407324516748709 1107 90-91 1.9348858091521848 1107 92-93 2.1980078764179005 1107 94-95 2.1472294688086677 1107 96-97 2.784186771753461 1107 98-99 3.2933580866733188 1107 100-101 3.6040019554887515 1108 1 0.18814782292709253 1108 2 0.19810254118171144 1108 3 0.2771703887154402 1108 4 0.15784612164284084 1108 5 0.20201321418525708 1108 6 -0.044610624506141505 1108 7 0.13176592908489226 1108 8 0.12182907393210485 1108 9 0.23707020713023752 1108 10-11 0.3189514048511768 1108 12-13 0.30866723676999896 1108 14-15 0.3391934316685692 1108 16-17 0.274497643555641 1108 18-19 0.28711144441796677 1108 20-21 0.27894020780012596 1108 22-23 0.42020587801653875 1108 24-25 0.4078073899069068 1108 26-27 0.5326121715193679 1108 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-0.9810757979097389 2316 60-61 -0.4494307262800348 2316 62-63 -0.6949826173283284 2316 64-65 -0.9100842472379398 2316 66-67 -0.7207703823828915 2316 68-69 -0.48510583595393086 2316 70-71 -0.6896697972389063 2316 72-73 -0.5096341781949114 2316 74-75 -0.19888481908601108 2316 76-77 -0.27316463906965893 2316 78-79 -0.3280660019286472 2316 80-81 -0.026896824495452165 2316 82-83 -0.16893649122793164 2316 84-85 -0.044038490927675866 2316 86-87 0.2066351475528414 2316 88-89 0.45142572898580013 2316 90-91 0.628331187303445 2316 92-93 0.3305078764179008 2316 94-95 0.822523586455727 2316 96-97 0.8079262675517818 2316 98-99 1.1915513639842423 2316 100-101 1.3255775857408487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 8.0 5 23.0 6 82.0 7 176.0 8 396.0 9 675.0 10 1237.0 11 1883.0 12 2510.0 13 3529.0 14 4758.0 15 6441.0 16 8392.0 17 10727.0 18 9425.0 19 6670.0 20 6061.0 21 6455.0 22 6970.0 23 7442.0 24 8626.0 25 9509.0 26 10786.0 27 12567.0 28 15060.0 29 18140.0 30 21816.0 31 25890.0 32 31060.0 33 38302.0 34 49120.0 35 65383.0 36 82734.0 37 108674.0 38 70951.0 39 4406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.14961629625795 26.238911715409195 16.87124269471005 19.7402292936228 2 14.279034844707347 24.59324488767117 40.602407253133194 20.525313014488294 3 15.918134957968356 30.8852677633562 33.71193784005139 19.484659438624053 4 10.627550857301179 21.936192982963583 41.76715325466937 25.669102905065866 5 9.750380981408108 41.47378847912222 36.98552270649193 11.790307832977751 6 23.51424217559489 43.83280533790439 18.99093755851463 13.66201492798609 7 22.535230564769893 37.47996230706347 24.393693283624124 15.591113844542518 8 23.52702976310994 36.56443193425962 22.03088202384885 17.877656278781586 9 24.74961446945974 18.18060031634056 24.47102774145325 32.598757472746456 10-11 21.294530109440437 30.147650965843443 29.898825825446384 18.65899309926974 12-13 22.744475077144166 29.695366174090825 29.351014710292638 18.20914403847237 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31.747346432614272 33.70120028105776 17.760536049920486 40-41 16.80083124950045 30.940750636568126 34.80727876165138 17.451139352280038 42-43 17.178842498605093 30.588128725615793 34.07878000379079 18.154248771988318 44-45 17.03666546653341 30.614663598588155 33.862026286724266 18.48664464815417 46-47 18.225765201267798 30.90886394290638 32.727215985726595 18.13815487009923 48-49 17.405048356871124 30.871139176144453 32.726328881527564 18.99748358545686 50-51 16.681179563608353 30.774927803412155 33.039624775646345 19.50426785733315 52-53 17.279303746306443 31.75028581780428 32.594190477205366 18.376219958683915 54-55 17.62144782892644 31.01203098858708 32.163294447903525 19.203226734582966 56-57 17.77124528267419 31.310179680827904 31.76231223939582 19.156262797102087 58-59 17.32093952232271 30.662275246731934 32.64503636089617 19.371748870049185 60-61 17.82247174932675 31.917361738015828 31.46302179826972 18.797144714387713 62-63 17.15637544965877 31.50222184333074 32.186129425608975 19.15527328140152 64-65 17.344383432766975 31.224396281247763 31.812549190347806 19.618671095637456 66-67 17.168643289213588 31.234086862798975 31.00870546129354 20.588564386693896 68-69 17.197885173378637 30.579050246161433 31.50318320073803 20.7198813797219 70-71 17.695965972838557 30.843432736528502 31.620507027844972 19.840094262787968 72-73 17.70494773073603 29.912070112515547 31.831730571620387 20.551251585128036 74-75 16.992877009287746 31.06729163463427 30.886134144837698 21.053697211240287 76-77 16.879082703722254 30.685262457622088 31.93836991750484 20.497284921150822 78-79 17.063665440174642 30.077319234510654 31.581535332561007 21.2774799927537 80-81 17.14632805946837 30.1940059705855 31.081449320811643 21.57821664913448 82-83 17.17540692116112 30.223144421237237 31.238087698893562 21.363360958708082 84-85 16.701732324700888 29.782922770806085 31.245861009068864 22.269483895424163 86-87 16.4594375088962 29.98333793842573 31.674586664413596 21.882637888264476 88-89 17.148002624500105 29.563912819099787 31.051916082979265 22.236168473420847 90-91 17.07948657640727 29.14751375622351 31.65076965585274 22.122230011516482 92-93 17.314320336619556 29.217335176378175 31.371672928553597 22.096671558448676 94-95 16.798399115829664 28.442487063985126 31.71770791627784 23.041405903907368 96-97 17.301834257642486 28.414323924815076 31.2108475278092 23.07299428973324 98-99 16.70439512427556 28.575614984007903 30.878826951971956 23.841162939744585 100-101 16.456176994006526 28.418313210459733 31.64606679139753 23.479443004136215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2516.0 1 1524.0 2 625.5 3 718.0 4 620.5 5 629.0 6 1076.0 7 2144.0 8 3084.5 9 3160.0 10 2435.5 11 1690.0 12 1513.0 13 1466.5 14 1449.5 15 1441.5 16 1401.5 17 1467.0 18 1599.5 19 1673.5 20 1851.5 21 2089.5 22 2212.0 23 2439.0 24 2804.5 25 3509.5 26 5162.0 27 7100.5 28 7715.5 29 8738.5 30 11633.5 31 14236.5 32 17556.5 33 22812.0 34 28888.0 35 33157.0 36 38186.5 37 43527.5 38 42745.5 39 39966.0 40 37065.5 41 33221.5 42 30179.5 43 28475.0 44 27335.0 45 25776.0 46 22557.0 47 18755.0 48 17036.0 49 14295.5 50 10114.0 51 6417.0 52 3687.5 53 2276.0 54 1624.5 55 1254.0 56 1117.0 57 1030.0 58 993.5 59 1064.5 60 1090.5 61 1113.0 62 935.0 63 575.0 64 318.0 65 221.0 66 161.5 67 136.0 68 121.5 69 95.5 70 81.0 71 70.5 72 56.0 73 57.0 74 50.5 75 47.0 76 42.5 77 32.5 78 29.5 79 26.5 80 21.0 81 13.0 82 6.0 83 0.5 84 1.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04384315719446419 3 1.5223318470300066E-4 4 0.0 5 0.10458419789096146 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012178654776240053 26-27 0.011874188406834053 28-29 0.0 30-31 0.0 32-33 4.5669955410900204E-4 34-35 0.01606060098616657 36-37 0.0031968968787630136 38-39 0.012559237737997555 40-41 0.006774376719283529 42-43 0.004947578502847522 44-45 0.0023596143628965105 46-47 1.5223318470300066E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.611659235150033E-5 68-69 1.5223318470300066E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.5669955410900204E-4 84-85 0.0036535964328720163 86-87 8.372825158665037E-4 88-89 0.0 90-91 3.805829617575017E-4 92-93 4.5669955410900204E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.133991082180041E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 656887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.03749204413671 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51179629691015 13.994802371526255 2 9.31579896872877 3.5469889750390187 3 3.773284151332252 2.155016010337646 4 2.0990174733193716 1.5984011379528589 5 1.373596603329428 1.3074917203868597 6 1.054034189743702 1.2039700500896287 7 0.7683460621612623 1.023916743187716 8 0.6491748638759887 0.9886929045034132 9 0.5196509307705284 0.8903565414244953 >10 5.090950466441031 21.163269037698015 >50 1.0089162079128957 13.41492903348713 >100 0.78107063005618 28.39915911828108 >500 0.04476965740342485 5.482280396578702 >1k 0.00799458167918301 2.9065020053153288 >5k 0.0015989163358366017 1.9242239541918469 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6873 1.0462986784637236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5770 0.8783854757363139 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 0.673022909571966 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4267 0.6495789991277039 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1971 0.3000516070496143 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1537 0.23398240488851202 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1294 0.19698974100568287 TruSeq Adapter, Index 11 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.19379284412691986 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.1823753552741948 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1065 0.1621283417086957 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1061 0.1615194089698837 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1010 0.15375551655003067 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 991 0.15086308604067367 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 862 0.13122500521398658 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 860 0.13092053884458058 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 856 0.13031160610576858 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 855 0.13015937292106558 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 833 0.12681024285759956 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 830 0.12635354330349055 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 778 0.11843741769893452 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 777 0.11828518451423152 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 775 0.11798071814482552 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 756 0.1150882876354685 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 755 0.1149360544507655 RNA PCR Primer, Index 11 (95% over 23bp) CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 753 0.1146315880813595 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.10960789298616047 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 718 0.10930342661675448 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 704 0.10717216203091247 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 698 0.10625876292269447 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 695 0.10580206336858547 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 682 0.10382303196744647 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 659 0.10032166871927745 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5223318470300066E-4 0.0 16-17 0.0 0.0 0.0 3.805829617575016E-4 0.0 18-19 0.0 0.0 0.0 5.328161464605023E-4 0.0 20-21 0.0 0.0 0.0 0.0012939820699755058 0.0 22-23 0.0 0.0 0.0 0.0046431121334415204 0.0 24-25 0.0 0.0 0.0 0.012711470922700557 0.0 26-27 0.0 0.0 0.0 0.02542294184540111 0.0 28-29 0.0 0.0 0.0 0.050922000283153725 0.0 30-31 0.0 0.0 0.0 0.11683896925955302 0.0 32-33 0.0 0.0 0.0 0.21495325680063693 0.0 34-35 0.0 0.0 0.0 0.3668819751342316 0.0 36-37 0.0 0.0 0.0 0.573538523368555 0.0 38-39 0.0 0.0 0.0 0.8343900853571466 0.0 40-41 0.0 0.0 0.0 1.0981340778550952 0.0 42-43 0.0 0.0 0.0 1.360507971690717 0.0 44-45 0.0 0.0 0.0 1.674488915140656 0.0 46-47 0.0 0.0 0.0 2.049972065210607 0.0 48-49 0.0 0.0 0.0 2.4163973407907298 0.0 50-51 0.0 0.0 0.0 2.79743700210234 0.0 52-53 0.0 0.0 0.0 3.1504657574285986 0.0 54-55 0.0 0.0 0.0 3.5447497058093704 0.0 56-57 0.0 0.0 0.0 4.030754147973701 0.0 58-59 0.0 0.0 0.0 4.521858401825581 0.0 60-61 0.0 0.0 0.0 5.038005014561104 0.0 62-63 0.0 0.0 0.0 5.529033151820633 0.0 64-65 0.0 0.0 0.0 6.069308724331583 0.0 66-67 0.0 0.0 0.0 6.657766099801032 0.0 68-69 0.0 0.0 0.0 7.290980031573163 0.0 70-71 0.0 0.0 0.0 8.009825129740731 0.0 72-73 0.0 0.0 0.0 8.679270559472176 0.0 74-75 0.0 0.0 0.0 9.42315801652339 0.0 76-77 0.0 0.0 0.0 10.18158374271374 0.0 78-79 0.0 0.0 0.0 11.029598698101804 0.0 80-81 0.0 0.0 0.0 11.929829635843 0.0 82-83 0.0 0.0 0.0 12.778834106931633 0.0 84-85 0.0 0.0 0.0 13.626164012988536 0.0 86-87 0.0 0.0 0.0 14.520077273564556 0.0 88-89 0.0 0.0 0.0 15.455930776526252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 35 3.37669E-6 67.987465 1 ACGCAGT 30 1.3017048E-4 63.32455 7 TGGGCGC 30 1.3017048E-4 63.32455 6 GTATGGT 80 1.8189894E-12 59.489033 1 CGTCAGA 50 2.7937936E-5 47.591225 2 GTGGTAC 145 0.0 39.385845 1 TGGTACT 160 1.8189894E-12 35.69342 2 CATTCCG 180 0.0 34.3008 9 GTCAGAT 70 2.0348336E-4 33.978184 3 ATGGGCG 100 1.2833298E-6 33.29862 5 TACCTGG 75 3.0425814E-4 31.727488 2 GACCGCG 75 3.0795255E-4 31.66228 7 GCCTATC 105 1.9020899E-6 31.66228 7 TACAATC 90 2.4136867E-5 31.662275 7 GCCACCG 55 2.7335518E-6 30.227686 28-29 GGTACTC 190 1.2732926E-11 30.043869 3 TAGGGTC 160 1.873559E-9 29.73091 4 TGTAGAT 130 2.994948E-7 29.273514 5 TACTCCC 215 1.8189894E-12 28.762926 5 ACGCACA 50 4.958325E-5 28.496048 88-89 >>END_MODULE