##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938344_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659732 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.177969235992798 31.0 31.0 34.0 28.0 34.0 2 31.374861307318728 33.0 31.0 34.0 28.0 34.0 3 31.379564732345862 33.0 31.0 34.0 28.0 34.0 4 35.058006887645284 37.0 35.0 37.0 32.0 37.0 5 35.07704492127106 37.0 35.0 37.0 32.0 37.0 6 35.090091127912544 37.0 35.0 37.0 32.0 37.0 7 35.04880466613716 37.0 35.0 37.0 32.0 37.0 8 35.02986667313394 37.0 35.0 37.0 32.0 37.0 9 36.515042471791574 39.0 37.0 39.0 32.0 39.0 10-11 36.3955545585177 39.0 37.0 39.0 32.0 39.0 12-13 36.28035399222715 39.0 36.0 39.0 32.0 39.0 14-15 37.37140687430654 40.0 37.0 41.0 32.0 41.0 16-17 37.332788162465974 40.0 37.0 41.0 32.0 41.0 18-19 37.434562822479435 40.0 37.0 41.0 32.0 41.0 20-21 37.45088535950961 40.0 37.0 41.0 32.0 41.0 22-23 37.31348259596321 40.0 37.0 41.0 32.0 41.0 24-25 37.19669123219732 40.0 36.5 41.0 31.5 41.0 26-27 37.00992448448764 39.0 36.0 41.0 31.0 41.0 28-29 36.81198880757641 39.0 36.0 41.0 31.0 41.0 30-31 36.51296132368901 39.0 35.5 40.5 30.0 41.0 32-33 36.17970706286795 38.5 35.0 40.0 30.0 41.0 34-35 35.90694024240146 38.0 35.0 40.0 30.0 41.0 36-37 35.63039082536545 38.0 35.0 40.0 28.5 41.0 38-39 35.41799776272789 38.0 35.0 40.0 27.0 41.0 40-41 35.203965398070736 38.0 35.0 40.0 26.5 41.0 42-43 34.78312405643504 38.0 34.0 40.0 24.5 41.0 44-45 34.27348074672746 38.0 33.5 40.0 23.5 41.0 46-47 33.72521645152881 38.0 33.0 40.0 20.0 41.0 48-49 33.20222529754507 38.0 33.0 40.0 15.0 41.0 50-51 32.50993282120619 37.5 32.0 39.5 12.0 40.5 52-53 32.604737226631414 37.5 32.5 39.5 9.0 40.0 54-55 32.73237011392505 38.0 32.5 40.0 8.0 41.0 56-57 32.466390140238765 37.5 32.0 40.0 4.0 41.0 58-59 32.1613928079887 37.0 31.0 40.0 2.0 41.0 60-61 31.761559997089726 36.5 31.0 39.5 2.0 41.0 62-63 31.190932833332326 36.0 30.0 39.0 2.0 40.0 64-65 30.68050966149891 35.5 29.0 39.0 2.0 40.0 66-67 30.088367700823973 35.0 28.0 38.5 2.0 40.0 68-69 29.37715087338495 34.5 27.5 38.0 2.0 40.0 70-71 28.712165697586293 34.0 26.0 37.5 2.0 39.0 72-73 27.91287447023943 34.0 25.5 36.5 2.0 39.0 74-75 27.0764939399635 33.0 23.5 36.0 2.0 39.0 76-77 26.232221568758224 32.0 21.0 35.5 2.0 38.0 78-79 25.317744174907386 31.5 18.0 35.0 2.0 37.0 80-81 24.399250756367735 31.0 10.5 34.5 2.0 36.0 82-83 23.460090612551763 30.0 4.5 34.0 2.0 36.0 84-85 22.517191071526014 29.5 2.0 34.0 2.0 35.0 86-87 21.61134521290463 29.0 2.0 34.0 2.0 35.0 88-89 20.560598091346183 28.0 2.0 33.5 2.0 35.0 90-91 19.42070946990596 26.0 2.0 33.0 2.0 35.0 92-93 18.277101156227076 24.5 2.0 32.0 2.0 34.0 94-95 16.970386459956465 19.0 2.0 32.0 2.0 34.0 96-97 15.431647244638732 2.0 2.0 31.0 2.0 34.0 98-99 13.593323501058006 2.0 2.0 31.0 2.0 34.0 100-101 9.51464003565084 2.0 2.0 22.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42925695283821597 1101 2 0.5466493317074317 1101 3 0.5756076454560848 1101 4 0.41935938695454666 1101 5 0.3280281221658896 1101 6 0.5402639578475572 1101 7 0.3885714109038716 1101 8 0.25173993517466897 1101 9 0.5887931295335704 1101 10-11 0.5113401144388305 1101 12-13 0.530781798784453 1101 14-15 0.7014920346258364 1101 16-17 0.8390761053308822 1101 18-19 0.7179176656860591 1101 20-21 0.7339810940510318 1101 22-23 0.8535760625168365 1101 24-25 0.8239782772827411 1101 26-27 0.8264010126973531 1101 28-29 0.9056840408963112 1101 30-31 1.0292933585213149 1101 32-33 1.007999340503467 1101 34-35 1.009881744416262 1101 36-37 1.1394442292629279 1101 38-39 1.1930293974002808 1101 40-41 1.1361990183957644 1101 42-43 1.4629229957803602 1101 44-45 1.4864267790542343 1101 46-47 1.5825592290160486 1101 48-49 1.5700430954441842 1101 50-51 1.6964038705788624 1101 52-53 1.88388208521652 1101 54-55 2.030049621659707 1101 56-57 2.2663452068374923 1101 58-59 2.21966874012535 1101 60-61 2.362054893111903 1101 62-63 2.05060946720333 1101 64-65 2.1286695439667547 1101 66-67 2.2346030255098626 1101 68-69 2.347771985209633 1101 70-71 2.3415078291808555 1101 72-73 2.5337160586653873 1101 74-75 2.4526545204181502 1101 76-77 3.0313931331979163 1101 78-79 2.97811872426826 1101 80-81 3.3267383646782314 1101 82-83 3.552881976842343 1101 84-85 3.450595208976722 1101 86-87 3.4187895693081067 1101 88-89 3.9910236956374554 1101 90-91 3.981776348014055 1101 92-93 3.918015979309814 1101 94-95 3.795729979004765 1101 96-97 4.112580481351925 1101 98-99 3.7816407403767194 1101 100-101 3.0526462196839983 1102 1 0.6546396757101931 1102 2 0.39037458955716886 1102 3 0.4323276798891875 1102 4 0.302892605865118 1102 5 0.2508007308453699 1102 6 0.3632921579208883 1102 7 0.3976180709460593 1102 8 0.4482769640442754 1102 9 0.6227665926641137 1102 10-11 0.5203894313562145 1102 12-13 0.3372085141959147 1102 14-15 0.557163931794129 1102 16-17 0.692165519808718 1102 18-19 0.4643561244858958 1102 20-21 0.5259783202733317 1102 22-23 0.4754163736900594 1102 24-25 0.591046043541489 1102 26-27 0.533480522344469 1102 28-29 0.37925610332224124 1102 30-31 0.5307944930070221 1102 32-33 0.4954256786386608 1102 34-35 0.8235032778583502 1102 36-37 0.8360038415716957 1102 38-39 0.9338366092223112 1102 40-41 0.6990859258461768 1102 42-43 0.7930782635668123 1102 44-45 0.9883902098240398 1102 46-47 1.2855285527350375 1102 48-49 1.3936053274318994 1102 50-51 1.145443675883051 1102 52-53 1.3121198861740595 1102 54-55 1.1801816683569442 1102 56-57 1.5142170498055378 1102 58-59 1.430649870997712 1102 60-61 1.0547447666977803 1102 62-63 1.2707847146912457 1102 64-65 1.4936843427615933 1102 66-67 1.6647232225437278 1102 68-69 1.7145881453873244 1102 70-71 1.3209754976665984 1102 72-73 1.7690793332109287 1102 74-75 1.6951594754903923 1102 76-77 2.2662648858322108 1102 78-79 2.6584235741282427 1102 80-81 2.994994285524605 1102 82-83 3.310196354256778 1102 84-85 3.146061517142364 1102 86-87 3.453793900014677 1102 88-89 3.285794699564317 1102 90-91 3.2515700682238453 1102 92-93 3.835478822910197 1102 94-95 3.8759197861687618 1102 96-97 3.860069734291754 1102 98-99 3.6271567321085243 1102 100-101 2.6348313401201526 1103 1 0.6596746637392492 1103 2 0.6207715315726041 1103 3 0.4881441186113271 1103 4 0.5428251986784431 1103 5 0.437223731492729 1103 6 0.5956671309477386 1103 7 0.697163894474869 1103 8 0.7598605191568453 1103 9 0.5131682614030026 1103 10-11 0.7947500392539624 1103 12-13 0.7887724431306609 1103 14-15 0.7094312177300779 1103 16-17 0.7597936294276053 1103 18-19 0.8946960375552848 1103 20-21 0.7931486570009909 1103 22-23 0.7991738105728885 1103 24-25 0.7696750882295831 1103 26-27 0.8919896521135797 1103 28-29 0.7065328940825353 1103 30-31 0.5188132765950186 1103 32-33 0.7917141731772404 1103 34-35 0.7596231516753846 1103 36-37 0.8869830953659203 1103 38-39 1.119635374108924 1103 40-41 1.3299904768588249 1103 42-43 1.5252605712354068 1103 44-45 1.6651593915871032 1103 46-47 1.4060394498877002 1103 48-49 1.067695176279507 1103 50-51 1.3271913305034246 1103 52-53 1.3147940813466406 1103 54-55 0.9926720350890292 1103 56-57 1.139467514771269 1103 58-59 0.8354269443338467 1103 60-61 1.2564448458190185 1103 62-63 1.4219653680066386 1103 64-65 1.5245698841255653 1103 66-67 1.4427573166055225 1103 68-69 1.2290457538707642 1103 70-71 1.5343408220123038 1103 72-73 1.9032335065994737 1103 74-75 1.8853972502311933 1103 76-77 1.6417028784133088 1103 78-79 2.0464952764679722 1103 80-81 2.4387128810712078 1103 82-83 2.323089520031189 1103 84-85 2.380946277569411 1103 86-87 2.4146031972742072 1103 88-89 2.729631463197318 1103 90-91 2.3322814401039516 1103 92-93 2.6014006881163745 1103 94-95 2.9715460256492356 1103 96-97 3.0875433006046062 1103 98-99 2.510478591149411 1103 100-101 1.7020073283649975 1104 1 0.7190107009932696 1104 2 0.6668743181130843 1104 3 0.7564706921429476 1104 4 0.7688748456665806 1104 5 0.6291598513665448 1104 6 0.7471383266815508 1104 7 0.6426254387140133 1104 8 0.6248804230174372 1104 9 0.7848090154964424 1104 10-11 0.8644957017145245 1104 12-13 0.868749347141474 1104 14-15 0.9419238007254194 1104 16-17 0.8167801661558585 1104 18-19 0.8503277973570036 1104 20-21 0.9972125183499543 1104 22-23 1.031830694304297 1104 24-25 1.0848367601538698 1104 26-27 1.0424163645966829 1104 28-29 1.1342875132803627 1104 30-31 0.907835170841679 1104 32-33 0.8227411936068663 1104 34-35 0.6107056588107298 1104 36-37 0.8396949963723799 1104 38-39 0.9184491227945202 1104 40-41 1.005456621518583 1104 42-43 0.8657574166612747 1104 44-45 0.4590863669513823 1104 46-47 0.7845930400935046 1104 48-49 0.5125511549485751 1104 50-51 0.5206836130631345 1104 52-53 0.4788859207637941 1104 54-55 0.7416322461450591 1104 56-57 0.7669484558858812 1104 58-59 0.6579164290867396 1104 60-61 0.7882206081744272 1104 62-63 0.8245209485984901 1104 64-65 0.8407619563803337 1104 66-67 0.9266182900169682 1104 68-69 1.1569717152647847 1104 70-71 1.5106979460922183 1104 72-73 1.8748916296278644 1104 74-75 1.5143874109642539 1104 76-77 1.5298516316054354 1104 78-79 1.8973501097261938 1104 80-81 1.7139987643519667 1104 82-83 1.8467370902580171 1104 84-85 2.1432477083941066 1104 86-87 2.235109337427428 1104 88-89 2.506461773397106 1104 90-91 2.344197937775572 1104 92-93 2.565350759469837 1104 94-95 2.788223958652086 1104 96-97 3.0888389292661618 1104 98-99 2.46119775894271 1104 100-101 1.6326137389102868 1105 1 0.3674097157715934 1105 2 0.24194615719354573 1105 3 0.2525708563465585 1105 4 0.13864085551879413 1105 5 0.051214449067693124 1105 6 0.1971075385042127 1105 7 0.33689883772879625 1105 8 0.3148434772046329 1105 9 0.4470627100777236 1105 10-11 0.3248046097604984 1105 12-13 0.22491724533523438 1105 14-15 0.37785873504397216 1105 16-17 0.38094785909510875 1105 18-19 0.33357389752119815 1105 20-21 0.4577779945404288 1105 22-23 0.45447539873779164 1105 24-25 0.4874948061308757 1105 26-27 0.36402453372640053 1105 28-29 0.24071197961863078 1105 30-31 0.21176579481540614 1105 32-33 0.34308704746565155 1105 34-35 0.620917070961795 1105 36-37 0.5705806696071889 1105 38-39 0.7440597549784229 1105 40-41 0.7322372044414038 1105 42-43 0.6545505201095452 1105 44-45 0.7092146510236361 1105 46-47 0.8185113487962923 1105 48-49 1.2767650301538254 1105 50-51 1.006700649187934 1105 52-53 1.2500527926849756 1105 54-55 1.2355515811204327 1105 56-57 1.3957200076100236 1105 58-59 1.3712784980522628 1105 60-61 1.4961947461054663 1105 62-63 1.7152229190418424 1105 64-65 1.651491636183291 1105 66-67 1.6437775675210737 1105 68-69 1.6346759435078049 1105 70-71 1.7145413368968185 1105 72-73 2.0144390434209676 1105 74-75 2.124403831325502 1105 76-77 2.2318733885840913 1105 78-79 2.7592907911711855 1105 80-81 2.8585749137772574 1105 82-83 3.3772430426389732 1105 84-85 3.2671957790016606 1105 86-87 3.5522532208428608 1105 88-89 3.4502476929373387 1105 90-91 3.0675918211910087 1105 92-93 3.002414593624188 1105 94-95 3.1357198535617776 1105 96-97 3.2872174185929293 1105 98-99 3.461505640716112 1105 100-101 2.6759480985161996 1106 1 0.39522947973805245 1106 2 0.253690192308234 1106 3 0.16931651397836944 1106 4 0.2103902705586762 1106 5 -0.1371384398667388 1106 6 -0.21403189549350543 1106 7 0.22999630830999962 1106 8 0.06663465071149233 1106 9 0.17509681709553604 1106 10-11 0.173118472888369 1106 12-13 0.3476617330467988 1106 14-15 0.45699380532458633 1106 16-17 -0.04604420765012662 1106 18-19 0.42489065556120664 1106 20-21 0.45935674639778057 1106 22-23 0.4800204363972753 1106 24-25 0.36376506624599614 1106 26-27 0.15746956455422634 1106 28-29 0.21533453089870846 1106 30-31 0.23256496989354503 1106 32-33 0.27142623040018776 1106 34-35 0.3446771262981727 1106 36-37 0.4147133488099328 1106 38-39 0.23067825850526447 1106 40-41 0.3252832241507164 1106 42-43 0.09801967520510857 1106 44-45 0.34353345001937896 1106 46-47 0.7109245868280922 1106 48-49 0.7402253041030349 1106 50-51 0.38529900788376636 1106 52-53 0.32486364134682333 1106 54-55 0.10488558053974373 1106 56-57 0.6320193449398701 1106 58-59 0.6669865132868438 1106 60-61 0.9054351946033563 1106 62-63 0.8778845302867495 1106 64-65 0.7663295552623843 1106 66-67 0.8425199032625628 1106 68-69 1.0914678855754083 1106 70-71 0.8512981813572047 1106 72-73 1.1604367633574348 1106 74-75 1.5579777668688735 1106 76-77 1.7196180470869358 1106 78-79 1.960185096990049 1106 80-81 2.0222036306223963 1106 82-83 2.2719929188152967 1106 84-85 2.2024210526946817 1106 86-87 1.8104085042709848 1106 88-89 1.957388903164322 1106 90-91 2.0623122358722235 1106 92-93 2.5425793645076915 1106 94-95 2.759611845158844 1106 96-97 2.8228391415353205 1106 98-99 2.9462902729170963 1106 100-101 2.520253703885876 1107 1 0.3273522445072281 1107 2 0.265681789377453 1107 3 0.19205361496725004 1107 4 0.1804799359785676 1107 5 0.257996058263096 1107 6 0.11130294080305703 1107 7 0.14184136646442624 1107 8 0.17926876456095187 1107 9 0.25677535375588434 1107 10-11 0.1883103568869373 1107 12-13 0.24023333728926133 1107 14-15 0.32886448217040964 1107 16-17 0.2649421119686721 1107 18-19 0.26906240326832886 1107 20-21 0.23062282212663376 1107 22-23 0.1380098814964228 1107 24-25 0.13048331187800244 1107 26-27 -0.06815937431957053 1107 28-29 0.09341312904391685 1107 30-31 0.34892096722919774 1107 32-33 0.3050123348219742 1107 34-35 0.20660672613421127 1107 36-37 0.36704618684857593 1107 38-39 0.3155540078519863 1107 40-41 -0.05537773808732993 1107 42-43 0.4299815545923096 1107 44-45 0.2616284441270764 1107 46-47 0.18641364764686585 1107 48-49 0.2386328462457854 1107 50-51 0.5261546721764319 1107 52-53 0.6122367007309535 1107 54-55 0.5400918110054889 1107 56-57 0.4437947202536989 1107 58-59 0.2565658543741023 1107 60-61 0.4908786541514445 1107 62-63 0.5954528040993168 1107 64-65 0.5070663488269673 1107 66-67 0.8139212456819962 1107 68-69 0.897032265346887 1107 70-71 0.8105183483910743 1107 72-73 0.7481459399726909 1107 74-75 0.9955245209806769 1107 76-77 0.9281664920323642 1107 78-79 1.0921930900217625 1107 80-81 1.2917358333174853 1107 82-83 1.3639097093056378 1107 84-85 1.3275980778522367 1107 86-87 1.196276209348607 1107 88-89 1.4452476929373397 1107 90-91 1.5302929706162978 1107 92-93 1.7739950533943016 1107 94-95 1.658478474251428 1107 96-97 1.4675335105469482 1107 98-99 1.59673552577358 1107 100-101 1.2418963743782676 1108 1 0.39422097981968207 1108 2 0.21843609372736594 1108 3 0.2901649765326404 1108 4 0.21334550597520519 1108 5 0.21499955929422043 1108 6 0.3594119044019308 1108 7 0.1880050996187137 1108 8 0.38444645388040755 1108 9 0.394257488905275 1108 10-11 0.4480101314780782 1108 12-13 0.313085478673365 1108 14-15 0.3469019863668521 1108 16-17 0.4009279160068431 1108 18-19 0.37466405306930284 1108 20-21 0.3012237525376449 1108 22-23 0.36589151786877494 1108 24-25 0.4148524068854442 1108 26-27 0.32927000556627917 1108 28-29 0.38894427862906156 1108 30-31 0.4704614209244653 1108 32-33 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0.09902405336930897 2316 82-83 0.3278692468778921 2316 84-85 0.3433535093850253 2316 86-87 0.7582694323902679 2316 88-89 -0.15903076032158125 2316 90-91 -0.27961932756987196 2316 92-93 0.12604010023504486 2316 94-95 0.4730230227864105 2316 96-97 0.4344210927673995 2316 98-99 0.3258585076352496 2316 100-101 0.2036165258632181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5125.0 3 902.0 4 747.0 5 713.0 6 737.0 7 921.0 8 1156.0 9 1481.0 10 1962.0 11 2694.0 12 3738.0 13 5141.0 14 6229.0 15 7789.0 16 9989.0 17 12085.0 18 10437.0 19 8019.0 20 8512.0 21 9339.0 22 10319.0 23 11649.0 24 13208.0 25 15023.0 26 16959.0 27 19606.0 28 22248.0 29 25872.0 30 29621.0 31 33900.0 32 39829.0 33 46919.0 34 55572.0 35 65684.0 36 72804.0 37 64156.0 38 18370.0 39 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.49774402909556 25.44400398694352 16.62448926796978 19.433762715991143 2 14.355808128286462 24.177891934182835 41.100551306699984 20.365748630830723 3 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18.2642233714018 30.94825508148108 32.935441274346154 17.852080272770962 48-49 17.608649841802617 30.852658883385715 32.8738256478361 18.664865626975573 50-51 16.972658559435345 30.73168217470265 33.01885219310613 19.276807072755883 52-53 17.509209150029562 31.523185835341916 32.65632816408204 18.311276850546484 54-55 17.90084670417501 30.849864819900084 32.39312358879479 18.856164887130113 56-57 17.803495985119856 31.09269620765503 32.2269737490355 18.876834058189612 58-59 17.669493195412475 30.19302654835062 32.896595735650344 19.24088452058657 60-61 18.029596078110362 31.06269384339727 32.296773293799134 18.61093678469323 62-63 17.553146737066623 30.52112491368653 33.02696315295998 18.898765196286874 64-65 17.779603555314363 30.35629694924195 32.546724704159615 19.317374791284067 66-67 17.68019323891184 30.50008223416197 31.701231541841274 20.11849298508492 68-69 17.688334957836194 29.94524692696104 32.262550989936166 20.103867125266603 70-71 18.220468740525135 30.334273239949063 32.248499181371656 19.196758838154146 72-73 18.32729990388959 29.53644161065279 32.34835323758675 19.787905247870867 74-75 17.63201079356176 30.766198869859508 31.582765168024075 20.019025168554656 76-77 17.819054397066523 30.394325867009854 32.17651173912813 19.61010799679549 78-79 18.066905876093156 29.976063898325382 31.701994776199722 20.25503544938174 80-81 18.332205229500282 30.075197658192877 31.182962777121297 20.409634335185544 82-83 18.59326039599608 30.028287599692565 31.29099693323611 20.087455071075247 84-85 18.196715223074236 29.677366054273325 31.253964993606743 20.871953729045696 86-87 18.257305438417255 29.888330397717798 31.432863331686157 20.421500832178793 88-89 19.04526921161486 29.477934660914872 31.11038847337219 20.366407654098076 90-91 18.977600857545944 29.35624165632512 31.333631008109236 20.332526478019698 92-93 19.608404923134763 29.297472489657565 31.028513130229012 20.065609456978663 94-95 19.368209755816526 28.58262607150546 31.188199870828065 20.86096430184995 96-97 19.926137164740503 28.28020272861927 30.527773608590646 21.26588649804958 98-99 19.696522952545614 28.48036358427025 30.001766076029195 21.821347387154944 100-101 20.28198512672636 27.767187737137654 30.863484595538015 21.087342540597966 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2051.0 1 1390.5 2 753.5 3 758.0 4 645.5 5 632.5 6 984.5 7 1876.0 8 2840.0 9 3128.0 10 2545.0 11 1831.5 12 1641.5 13 1597.5 14 1521.5 15 1486.5 16 1523.0 17 1605.5 18 1667.5 19 1775.5 20 1937.5 21 2157.0 22 2427.0 23 2751.5 24 3089.0 25 3763.0 26 5361.5 27 7358.0 28 8159.5 29 9196.0 30 11925.0 31 14523.5 32 17942.5 33 23175.0 34 28991.5 35 33249.5 36 38111.5 37 42856.0 38 42217.0 39 39672.0 40 37050.5 41 33709.5 42 30591.0 43 28274.5 44 26372.0 45 24298.0 46 21350.5 47 17821.0 48 15775.0 49 13463.5 50 9877.5 51 6442.0 52 4157.0 53 2937.0 54 2247.0 55 1805.5 56 1497.5 57 1353.0 58 1324.5 59 1288.5 60 1300.5 61 1353.5 62 1221.5 63 896.0 64 600.5 65 440.0 66 358.5 67 312.0 68 263.5 69 230.0 70 192.5 71 150.5 72 126.5 73 110.5 74 93.0 75 74.0 76 68.0 77 50.0 78 39.0 79 43.5 80 29.0 81 19.5 82 12.0 83 4.5 84 4.5 85 3.0 86 2.0 87 2.0 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39212892507866826 2 0.031527953775169315 3 0.0365299849029606 4 0.04744350736359613 5 0.06548113476381319 6 0.054870765704861976 7 0.0365299849029606 8 0.03971309562064596 9 0.036833138304644915 10-11 0.037591021808855714 12-13 0.029178514912115826 14-15 0.02789011295495747 16-17 0.023039658528008343 18-19 0.02622276924569371 20-21 0.036908926655065996 22-23 0.035620524697907635 24-25 0.04388145489380536 26-27 0.03789417521054003 28-29 0.03296793243316983 30-31 0.036075254800434116 32-33 0.02986061006590555 34-35 0.03183110717685363 36-37 0.034711064492854674 38-39 0.015991341938847898 40-41 0.013262961323689013 42-43 0.014475574930426295 44-45 0.011974559366530652 46-47 0.01985654781032298 48-49 0.017128167195164096 50-51 0.011216675862319852 52-53 0.009397755452213928 54-55 0.008564083597582048 56-57 0.00924617875137177 58-59 0.004547301025264804 60-61 0.007578835042108008 62-63 0.010989310811056611 64-65 0.006593586486633966 66-67 0.004623089375685885 68-69 0.0065177981362128865 70-71 0.013945056477478734 72-73 0.011216675862319852 74-75 0.012050347716951732 76-77 0.005532549580738845 78-79 0.008943025349687448 80-81 0.010686157409372291 82-83 0.011974559366530652 84-85 0.007957776794213408 86-87 0.0027283806151588825 88-89 0.016900802143900856 90-91 0.026601710997799108 92-93 0.01076194575979337 94-95 0.02213019832295538 96-97 0.01985654781032298 98-99 0.011747194315267412 100-101 0.12611181510067726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.21461564603309 #Duplication Level Percentage of deduplicated Percentage of total 1 77.45678743181989 17.20672761974365 2 8.128320286353306 3.611350220183846 3 3.179271525860265 2.1187888494408877 4 1.7263680673458865 1.5340241231869534 5 1.13502257131032 1.2607045085615471 6 0.8633815061028789 1.1507812988381174 7 0.670613447952806 1.042819399533282 8 0.5599740050428793 0.9951685835037885 9 0.4278753024640085 0.8554576849801291 >10 4.304563811968369 20.629913234223547 >50 0.8712905544453593 13.530097094560517 >100 0.6375796763117806 27.15385907077692 >500 0.033484891896331456 4.917482190403569 >1k 0.00410019084444875 2.0366418999924143 >5k 0.0013667302814829166 1.9561842220708299 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7195 1.0905943625593422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5691 0.8626230044927334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4293 0.6507187767153935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3886 0.5890270594726343 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1490 0.22584928425481862 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1325 0.2008391286158622 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1221 0.18507515172827754 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.18204361771143432 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 999 0.15142512414131798 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 986 0.1494546270303699 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 980 0.14854516682531693 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 830 0.12580866169899294 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 824 0.12489920149393996 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 802 0.12156451407541245 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 799 0.12110978397288595 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 785 0.11898771016109573 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 771 0.11686563634930548 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 764 0.11580459944341036 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 760 0.11519829264004171 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 753 0.11413725573414658 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 750 0.11368252563162011 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 746 0.11307621882825147 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 744 0.11277306542656715 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 729 0.11049941491393475 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 729 0.11049941491393475 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 709 0.10746788089709156 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.10549738378614346 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 675 0.10231427306845811 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.578835042108008E-5 0.0 14-15 0.0 0.0 0.0 3.031534016843203E-4 0.0 16-17 0.0 0.0 0.0 3.031534016843203E-4 0.0 18-19 0.0 0.0 0.0 3.7894175210540035E-4 0.0 20-21 0.0 0.0 0.0 9.094602050529609E-4 0.0 22-23 0.0 0.0 0.0 0.004395724324422644 0.0 24-25 0.0 0.0 0.0 0.012959807922004693 0.0 26-27 0.0 0.0 0.0 0.024176483784324545 0.0 28-29 0.0 0.0 0.0 0.05032346467959717 0.0 30-31 0.0 0.0 0.0 0.11444040913583092 0.0 32-33 0.0 0.0 0.0 0.21425366664039336 0.0 34-35 0.0 0.0 0.0 0.35772101398749795 0.0 36-37 0.0 0.0 0.0 0.5559075503386224 0.0 38-39 0.0 0.0 0.0 0.8168468408384011 0.0 40-41 0.0 0.0 0.0 1.0755124808255472 0.0 42-43 0.0 0.0 0.0 1.3337991790605883 0.0 44-45 0.0 0.0 0.0 1.6492302935131233 0.0 46-47 0.0 0.0 0.0 2.0381003195236853 0.0 48-49 0.0 0.0 0.0 2.4271219222350897 0.0 50-51 0.0 0.0 0.0 2.8104593986649125 0.0 52-53 0.0 0.0 0.0 3.175304517591992 0.0 54-55 0.0 0.0 0.0 3.5846373982162456 0.0 56-57 0.0 0.0 0.0 4.072183856475053 0.0 58-59 0.0 0.0 0.0 4.576252175125656 0.0 60-61 0.0 0.0 0.0 5.083200451092262 0.0 62-63 0.0 0.0 0.0 5.5696858724451745 0.0 64-65 0.0 0.0 0.0 6.111875731357582 0.0 66-67 0.0 0.0 0.0 6.718940418230433 0.0 68-69 0.0 0.0 0.0 7.378374855244251 0.0 70-71 0.0 0.0 0.0 8.094953708475563 0.0 72-73 0.0 0.0 0.0 8.75082609301959 0.0 74-75 0.0 0.0 0.0 9.448003128543105 0.0 76-77 0.0 0.0 0.0 10.196488877301693 0.0 78-79 0.0 0.0 0.0 11.020762976481357 0.0 80-81 0.0 0.0 0.0 11.89589105879357 0.0 82-83 0.0 0.0 0.0 12.722438808485869 0.0 84-85 0.0 0.0 0.0 13.525567957898055 0.0 86-87 0.0 0.0 0.0 14.357724045521515 0.0 88-89 0.0 0.0 0.0 15.212161908168772 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 30 1.3614244E-6 79.21814 4 CACGCAG 50 2.8148368E-5 47.530888 5 TACGTAC 45 9.4064936E-4 42.417053 1 ATGTATT 60 8.065093E-5 39.765987 1 CCACTAG 25 0.0016083496 38.036255 94-95 TATCACG 50 0.0016101656 38.024708 2 CGCGAGA 25 0.0016167642 37.995876 92-93 GTAAGCG 55 6.955452E-8 34.57841 94-95 ACGTACA 55 0.0025706233 34.567917 2 CGTATGA 60 0.0039371927 31.687256 2 CGTACAC 60 0.0039371927 31.687256 3 GTGTACG 75 3.0762568E-4 31.668034 9 ACGCGCA 30 0.0039532627 31.663227 92-93 CATTCCG 260 0.0 31.059032 9 GGTATCA 4250 0.0 30.54028 1 GGCATTC 265 0.0 30.473011 7 CCTTTAG 270 0.0 30.045414 1 ATCACGC 65 0.0058237594 29.249775 3 GTATCAA 6230 0.0 28.64683 1 GTTAGAG 85 6.2341226E-4 28.070108 1 >>END_MODULE