FastQCFastQC Report
Thu 26 May 2016
SRR938344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659732
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66161.0028314527717317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.8805090551921083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43610.6610259923726605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42470.6437462484766542No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19840.3007281744708457No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15660.2373691135188228No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.19235083336870124No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT12590.19083506636027964TruSeq Adapter, Index 11 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.18083100410469705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.17082694184911448No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10520.1594586892859525No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10200.15460823485900335No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10060.1524861610472131No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC8950.13566114725373332No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA8590.13020438602341558No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG8540.12944650251920478No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT8460.12823388891246748No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC8220.12459604809225565No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8200.12429289469057132No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8110.12292870438299187No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8020.12156451407541245No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7980.1209582072720438No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7950.12050347716951733No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7680.11641090624677901No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA7660.11610775284509468No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT7480.11337937222993578RNA PCR Primer, Index 11 (95% over 23bp)
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT7180.10883207120467098No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.10640684399119643No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7000.1061036905895121No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.10261742647014241No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6700.10155638956424731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT504.2249667E-757.0277141
AGACCGA805.2750693E-1132.65231794-95
AGGGTCT1753.6379788E-1232.5675055
TAGGGTC1753.6379788E-1232.5675054
GCCTATC1351.196895E-831.6628517
GGTATCA46000.031.6131861
GACCGTC404.497169E-429.68392494-95
CCTTTAG2450.029.0957721
CTTTAGG2350.028.3030452
GACATAT2700.028.1618331
GGATATG3900.028.0264421
GCCCAAC350.00839228927.14267390-91
CTCCGAC2800.027.13958794-95
CACTGAC2700.026.385717
GTGCTTA1457.887302E-726.203749
CATTCCG2550.026.075299
TTAGGCA3350.025.5193144
CATATAG3000.025.345651
GTGGTAC1501.0544863E-625.345651
GTACATG35400.025.1040081
Produced by FastQC (version 0.11.5)