##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938344_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659732 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78739548786477 31.0 31.0 34.0 30.0 34.0 2 32.056241019080474 33.0 31.0 34.0 30.0 34.0 3 32.07459847331947 33.0 31.0 34.0 30.0 34.0 4 35.75773647481098 37.0 35.0 37.0 35.0 37.0 5 35.66582794225534 37.0 35.0 37.0 33.0 37.0 6 35.70795868625442 37.0 35.0 37.0 33.0 37.0 7 35.64527717315516 37.0 35.0 37.0 33.0 37.0 8 35.6409466268121 37.0 35.0 37.0 33.0 37.0 9 37.135023009343186 39.0 37.0 39.0 33.0 39.0 10-11 37.09672942952592 39.0 37.0 39.0 33.0 39.0 12-13 37.01415726385866 39.0 37.0 39.0 33.0 39.0 14-15 37.93726619293895 40.0 37.0 41.0 32.5 41.0 16-17 38.010523212455965 40.0 37.0 41.0 33.0 41.0 18-19 38.23832859403515 40.0 37.5 41.0 33.0 41.0 20-21 38.268308191811215 40.0 38.0 41.0 33.0 41.0 22-23 38.12626945486956 40.0 37.0 41.0 33.0 41.0 24-25 37.998687345770705 40.0 37.0 41.0 33.0 41.0 26-27 37.777627885262504 40.0 37.0 41.0 32.5 41.0 28-29 37.64465798233222 39.5 36.5 41.0 32.0 41.0 30-31 37.32710100465037 39.0 36.0 41.0 32.0 41.0 32-33 37.09173497723318 39.0 35.5 41.0 31.5 41.0 34-35 36.90105072969024 39.0 35.0 41.0 31.0 41.0 36-37 36.71472128076249 39.0 35.0 41.0 31.0 41.0 38-39 36.480830852528 39.0 35.0 40.5 30.0 41.0 40-41 36.28093832040889 39.0 35.0 40.0 30.0 41.0 42-43 35.97568103411689 38.5 35.0 40.0 30.0 41.0 44-45 35.583873451643996 38.0 35.0 40.0 28.5 41.0 46-47 35.03356893405201 38.0 34.5 40.0 26.0 41.0 48-49 34.54379278252381 38.0 34.0 40.0 24.0 41.0 50-51 34.187607240515845 38.0 33.5 40.0 23.0 41.0 52-53 33.71110390279689 38.0 33.0 40.0 20.5 41.0 54-55 33.355245766462744 38.0 33.0 40.0 18.5 41.0 56-57 33.0217959716976 37.0 32.5 40.0 16.0 41.0 58-59 32.56679833629413 37.0 31.5 40.0 11.0 41.0 60-61 32.16022869892623 36.5 31.0 39.0 8.0 40.0 62-63 31.757768305918162 36.0 31.0 39.0 6.5 40.0 64-65 31.201212007299937 35.5 30.0 39.0 2.0 40.0 66-67 30.59763510031346 35.0 29.0 38.5 2.0 40.0 68-69 29.91117226388897 34.5 28.0 38.0 2.0 40.0 70-71 29.24819851091049 34.0 27.5 37.0 2.0 39.0 72-73 28.73943283030079 34.0 26.0 36.5 2.0 39.0 74-75 28.07236347486555 33.0 26.0 36.0 2.0 39.0 76-77 26.813449249089025 31.5 25.0 34.5 2.0 36.5 78-79 27.35134797160059 33.0 26.0 35.0 2.0 37.0 80-81 26.978093377310785 33.0 25.5 35.0 2.0 37.0 82-83 26.35683580605458 32.0 24.0 35.0 2.0 36.0 84-85 25.670510904427857 32.0 21.0 34.5 2.0 36.0 86-87 24.90436798578817 31.0 19.0 34.0 2.0 35.0 88-89 24.03664139377808 31.0 12.5 34.0 2.0 35.0 90-91 23.28204331455803 30.0 4.5 34.0 2.0 35.0 92-93 22.363289184092935 29.0 2.0 34.0 2.0 35.0 94-95 21.304645674304112 29.0 2.0 34.0 2.0 35.0 96-97 20.000529002685937 28.0 2.0 33.0 2.0 35.0 98-99 18.313969157172913 25.5 2.0 33.0 2.0 34.0 100-101 13.767933039476635 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3442390808321356 1101 2 0.3712522198670172 1101 3 0.24772563824716087 1101 4 0.24397879752190477 1101 5 0.18862539494216435 1101 6 0.31322723301639144 1101 7 0.3977387584892398 1101 8 0.3423242831442721 1101 9 0.29825816861589516 1101 10-11 0.5088945632205792 1101 12-13 0.4854713165272031 1101 14-15 0.5238659674576098 1101 16-17 0.5874535541353652 1101 18-19 0.34381587376721257 1101 20-21 0.27297586320639766 1101 22-23 0.3834253146562787 1101 24-25 0.3423266831795928 1101 26-27 0.4169595103333492 1101 28-29 0.6284894963638123 1101 30-31 0.5541268232722274 1101 32-33 0.5715807576605272 1101 34-35 0.5066145388168124 1101 36-37 0.6239755968739402 1101 38-39 0.9608159125235858 1101 40-41 0.808238346756994 1101 42-43 0.9562032780279495 1101 44-45 0.4947497413226358 1101 46-47 0.5578780194859121 1101 48-49 0.8039099730664461 1101 50-51 1.0810693275512122 1101 52-53 1.1329369665907976 1101 54-55 0.9450085540290516 1101 56-57 1.2373486523567578 1101 58-59 1.485353933273558 1101 60-61 1.2576556909453558 1101 62-63 1.186956618513463 1101 64-65 1.130580149049269 1101 66-67 1.1928288488269345 1101 68-69 1.339551166985924 1101 70-71 1.7126811800693496 1101 72-73 1.611217157502633 1101 74-75 1.5097124489151454 1101 76-77 1.462068816484308 1101 78-79 1.2487286256690346 1101 80-81 1.8229797029862738 1101 82-83 1.8662082736949124 1101 84-85 1.9340233755263405 1101 86-87 2.022020455793225 1101 88-89 1.8250939603924508 1101 90-91 1.8650013240771735 1101 92-93 2.538027778792358 1101 94-95 2.5923410313552395 1101 96-97 2.7386093250954318 1101 98-99 2.8556705158008135 1101 100-101 2.7201539413071796 1102 1 0.3863372683118733 1102 2 0.3363249385411322 1102 3 0.2683695585196517 1102 4 0.35633804955839565 1102 5 0.350081043724515 1102 6 0.37993871231136467 1102 7 0.4188210631690623 1102 8 0.4840260648447767 1102 9 0.4864552662454287 1102 10-11 0.39713045122797297 1102 12-13 0.23299510884458385 1102 14-15 0.5923362981322953 1102 16-17 0.5042229533096076 1102 18-19 0.49512093086345743 1102 20-21 0.5204305767686748 1102 22-23 0.6838474921249187 1102 24-25 0.3596388819563714 1102 26-27 0.5811079021267958 1102 28-29 0.5140167001089466 1102 30-31 0.3262785202184162 1102 32-33 0.5476609102714391 1102 34-35 0.5979366530518178 1102 36-37 0.5549725627224689 1102 38-39 0.7124950576474163 1102 40-41 0.5743376820068278 1102 42-43 0.6102475734512254 1102 44-45 0.39691429754476815 1102 46-47 0.38836672510944936 1102 48-49 0.4889958698415313 1102 50-51 0.3949392788241184 1102 52-53 0.7170655912327746 1102 54-55 0.5625978648887653 1102 56-57 0.7605126187525997 1102 58-59 1.026038663149052 1102 60-61 0.8848828165783615 1102 62-63 1.0038128975066059 1102 64-65 1.257856426873392 1102 66-67 1.1002938710649808 1102 68-69 1.4246136832592846 1102 70-71 1.6874727482102259 1102 72-73 1.6732937180182788 1102 74-75 1.5624062546763149 1102 76-77 1.6333588888044481 1102 78-79 1.9407237263911767 1102 80-81 2.343640892947537 1102 82-83 2.412277161154993 1102 84-85 2.588588418488012 1102 86-87 2.465955075408406 1102 88-89 2.348920817149679 1102 90-91 2.915181725902979 1102 92-93 3.061790870544886 1102 94-95 2.995649372349231 1102 96-97 3.0485789835806933 1102 98-99 3.1788740696370787 1102 100-101 2.483399792596563 1103 1 0.2824315561693318 1103 2 0.24135889902161267 1103 3 0.2338182376059521 1103 4 0.1016447214313061 1103 5 0.2845388577176706 1103 6 0.2661890847977233 1103 7 0.33438739656747174 1103 8 0.2336535784854803 1103 9 0.3055347882298278 1103 10-11 0.5095468344624905 1103 12-13 0.2939441527106368 1103 14-15 0.6372042067626822 1103 16-17 0.38449556194717616 1103 18-19 0.40837014221992973 1103 20-21 0.508277245969964 1103 22-23 0.5681943308717123 1103 24-25 0.549303798365294 1103 26-27 0.6338827970599539 1103 28-29 0.6048108924043802 1103 30-31 0.5574769501241903 1103 32-33 0.8295894134414326 1103 34-35 0.9009478841577732 1103 36-37 0.6133681483737874 1103 38-39 0.44054918890647343 1103 40-41 0.6017475403078762 1103 42-43 0.9605363146014554 1103 44-45 0.8378251166037387 1103 46-47 0.6616972155583909 1103 48-49 0.8654203090568942 1103 50-51 0.7364938570668116 1103 52-53 0.727975588252896 1103 54-55 0.7103028351412348 1103 56-57 0.8732732307605318 1103 58-59 0.8230335973345717 1103 60-61 0.559653416358465 1103 62-63 0.6381314786675318 1103 64-65 0.7096027456806127 1103 66-67 0.9567941433653537 1103 68-69 0.732762657252028 1103 70-71 1.0288680564234234 1103 72-73 0.8240171122167403 1103 74-75 0.6436776405310809 1103 76-77 0.5529429371403332 1103 78-79 0.9341936551820567 1103 80-81 1.1832405600217974 1103 82-83 1.4174894013136488 1103 84-85 1.3188209527310697 1103 86-87 0.9898327354747813 1103 88-89 1.1282855866191568 1103 90-91 1.8516355530076574 1103 92-93 1.7618468719423532 1103 94-95 1.5241355877850644 1103 96-97 1.7232288464029573 1103 98-99 2.1409806469755246 1103 100-101 2.2561034784133103 1104 1 0.28478809815521444 1104 2 0.23096100958192523 1104 3 0.3010688760316711 1104 4 0.3010119288821116 1104 5 0.2970961282599589 1104 6 0.2175279010813327 1104 7 0.21727383189870153 1104 8 0.40227777106472473 1104 9 0.480740398865251 1104 10-11 0.38572979976217425 1104 12-13 0.26790718035071137 1104 14-15 0.41807162076298 1104 16-17 0.5776146471206829 1104 18-19 0.49214474038726763 1104 20-21 0.5353187999732683 1104 22-23 0.6789518042080473 1104 24-25 0.40728458926209754 1104 26-27 0.5351732619840917 1104 28-29 0.5639187863506052 1104 30-31 0.8234235101362017 1104 32-33 0.8520563655219462 1104 34-35 0.6222914886342608 1104 36-37 0.5767971516785693 1104 38-39 0.7581800661785252 1104 40-41 0.7282004282453869 1104 42-43 0.5527935250566145 1104 44-45 0.49819202361891257 1104 46-47 0.4831516056740526 1104 48-49 0.5619779739209463 1104 50-51 0.6424575043553773 1104 52-53 0.7291327348654804 1104 54-55 0.6835358980825035 1104 56-57 0.8313037813088258 1104 58-59 0.7552126006393536 1104 60-61 0.7582953534172034 1104 62-63 0.6890928726888603 1104 64-65 0.743244494967076 1104 66-67 0.9894477410820421 1104 68-69 0.9786504445436002 1104 70-71 1.2510730661875797 1104 72-73 1.4412550760142437 1104 74-75 1.3674294243676428 1104 76-77 1.455601792480575 1104 78-79 1.4871430881098036 1104 80-81 1.5275515296777975 1104 82-83 1.6130706503747838 1104 84-85 1.8994597539928968 1104 86-87 1.8136507090364482 1104 88-89 1.9562721045701856 1104 90-91 2.2070180451212202 1104 92-93 2.467107781511224 1104 94-95 2.2087814492842384 1104 96-97 2.669354635046492 1104 98-99 2.8934263807895526 1104 100-101 2.670070918701727 1105 1 0.08476757270365454 1105 2 0.11699343979538668 1105 3 0.09263291544053942 1105 4 -0.01600367046107465 1105 5 -0.010939586025756398 1105 6 0.006834140818611445 1105 7 0.07359567097915942 1105 8 0.05555158058853493 1105 9 0.19035657292108255 1105 10-11 0.12208037447481956 1105 12-13 0.1022924430764931 1105 14-15 0.15229267987627537 1105 16-17 0.009008325281598673 1105 18-19 0.17902897684046337 1105 20-21 0.187899875506929 1105 22-23 0.12712503901921934 1105 24-25 -0.04881660121409226 1105 26-27 -0.2348647101012986 1105 28-29 -0.12404303630949443 1105 30-31 0.02524001259926223 1105 32-33 0.15778296650716328 1105 34-35 0.23572026531734735 1105 36-37 -0.019031050291864915 1105 38-39 -0.20790162511868715 1105 40-41 0.03733938555244265 1105 42-43 -0.15456484932762038 1105 44-45 -0.0733391750674528 1105 46-47 2.1338728325304146E-4 1105 48-49 -0.3371137748475235 1105 50-51 -0.3328093265148979 1105 52-53 -0.254472560701025 1105 54-55 -0.34150515282062344 1105 56-57 -0.13480356680283023 1105 58-59 -0.012670199032235985 1105 60-61 0.0432142778835356 1105 62-63 0.09949927662975 1105 64-65 0.12304231926921361 1105 66-67 0.2491973305730113 1105 68-69 0.4551488025074768 1105 70-71 0.5393017197179582 1105 72-73 0.7068287623853493 1105 74-75 0.5145712552379216 1105 76-77 0.6018456348451089 1105 78-79 0.6796974805564346 1105 80-81 0.8760480403510336 1105 82-83 1.355091381080861 1105 84-85 1.3757528574411744 1105 86-87 1.5641125316965478 1105 88-89 1.8685771127803683 1105 90-91 1.733957700293633 1105 92-93 1.7449454251893854 1105 94-95 1.5447830913203653 1105 96-97 1.7273646925177566 1105 98-99 2.0411583183921813 1105 100-101 1.8638824950564068 1106 1 -0.09127766990535235 1106 2 -0.04883848450991479 1106 3 -0.044217864221636205 1106 4 0.003733396369554498 1106 5 -0.0030148708202091257 1106 6 -0.12562730305084102 1106 7 -0.06931606408941349 1106 8 0.03369670193573171 1106 9 -0.23341928060832373 1106 10-11 -0.010258313165017796 1106 12-13 0.036402236248257225 1106 14-15 0.14562968522204756 1106 16-17 -0.09414426818392485 1106 18-19 0.24512093086345743 1106 20-21 0.04003626088037038 1106 22-23 0.2289721466689585 1106 24-25 0.16592460730497294 1106 26-27 0.10971843109185642 1106 28-29 0.3184184502577736 1106 30-31 -0.062413272570935874 1106 32-33 -0.0022783475609102766 1106 34-35 -0.06870984689251713 1106 36-37 0.10232362341140089 1106 38-39 -0.023047822450926958 1106 40-41 -0.07125717983499413 1106 42-43 0.0015106233372321753 1106 44-45 -0.06506860752804755 1106 46-47 -0.5599467722358042 1106 48-49 -0.1885648602260801 1106 50-51 -0.12402873352758803 1106 52-53 -0.1481944868850391 1106 54-55 -0.18056319623576655 1106 56-57 0.2676020283193665 1106 58-59 0.08464880491304427 1106 60-61 -0.05430967559291844 1106 62-63 -0.13698793110367546 1106 64-65 0.23512409413215352 1106 66-67 0.5264267971918564 1106 68-69 0.7076092643270862 1106 70-71 0.30825528157001614 1106 72-73 0.43870674055822434 1106 74-75 0.21214900796631397 1106 76-77 0.621543206193163 1106 78-79 0.7511053130443521 1106 80-81 0.5267809484140891 1106 82-83 0.7230846689837129 1106 84-85 0.7923832309616934 1106 86-87 0.8608783853967381 1106 88-89 0.8321985709962796 1106 90-91 1.1720533348686182 1106 92-93 0.8455051935964164 1106 94-95 0.826931346687477 1106 96-97 0.9894001402470849 1106 98-99 1.634747667848206 1106 100-101 2.0135821158997746 1107 1 -0.04333587557220753 1107 2 -0.10444334181380555 1107 3 -0.09897627996176084 1107 4 0.051869892757316904 1107 5 0.10032478179034143 1107 6 -0.10661413504345774 1107 7 -0.09496754741164182 1107 8 -0.05554037343445373 1107 9 0.04024163039234452 1107 10-11 -0.18116675196196041 1107 12-13 0.003010833881091912 1107 14-15 -0.018799274146708456 1107 16-17 0.005243957465509652 1107 18-19 -0.008672172584816451 1107 20-21 0.18258378355290716 1107 22-23 0.01850434936404355 1107 24-25 -0.18108671615662786 1107 26-27 -0.11178999745761331 1107 28-29 -0.07849705929799455 1107 30-31 -0.10473125176856257 1107 32-33 -0.1269296771709989 1107 34-35 -0.28022801054471813 1107 36-37 -0.08733564799302229 1107 38-39 -0.053016567647418356 1107 40-41 -0.1564249822636441 1107 42-43 -0.3509441596724443 1107 44-45 -0.2272472210444647 1107 46-47 -0.26136707248686264 1107 48-49 -0.10231492427281808 1107 50-51 -0.1551943839861636 1107 52-53 -0.15484612391941965 1107 54-55 -0.21722354362521656 1107 56-57 -0.19721735990627565 1107 58-59 0.05060566303673397 1107 60-61 -0.01868227384059651 1107 62-63 0.4567119203079102 1107 64-65 0.5368354227174912 1107 66-67 0.7549731926419767 1107 68-69 0.8713844346913824 1107 70-71 0.3526350530512907 1107 72-73 0.5938115210060353 1107 74-75 0.721640220755166 1107 76-77 0.6334835658795903 1107 78-79 0.8337204690621824 1107 80-81 0.6668813736843653 1107 82-83 0.7676775879774134 1107 84-85 0.8728218229584144 1107 86-87 0.9709803477885011 1107 88-89 0.951335733470021 1107 90-91 1.115768045121218 1107 92-93 1.4099454251893846 1107 94-95 1.2305589533893304 1107 96-97 1.4586003247016635 1107 98-99 1.6067905023002247 1107 100-101 1.2365549088495111 1108 1 -0.02166017656496777 1108 2 -0.06297194955444496 1108 3 -0.036573517504322695 1108 4 0.10738569535671161 1108 5 -0.007230731134704627 1108 6 0.09048720302185842 1108 7 0.1253058207721338 1108 8 -0.05751054273089551 1108 9 -0.1375973221758784 1108 10-11 0.01368529350348524 1108 12-13 0.15144975767370994 1108 14-15 0.21138057334261617 1108 16-17 0.15563098926254781 1108 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-0.1197521860926507 2316 92-93 -0.1958558504733574 2316 94-95 -0.1997480029590868 2316 96-97 -0.4999914632194056 2316 98-99 -0.575960145497266 2316 100-101 -1.196748858342037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 8.0 5 42.0 6 87.0 7 203.0 8 421.0 9 732.0 10 1339.0 11 1876.0 12 2890.0 13 3801.0 14 5005.0 15 6956.0 16 8774.0 17 11353.0 18 10107.0 19 7509.0 20 7129.0 21 7642.0 22 8373.0 23 9344.0 24 10378.0 25 11508.0 26 12939.0 27 15015.0 28 18132.0 29 21469.0 30 25034.0 31 29610.0 32 35534.0 33 43659.0 34 55274.0 35 70702.0 36 87640.0 37 94025.0 38 34443.0 39 771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.147241077642214 26.21571341715804 16.899072247635132 19.73797325756462 2 14.328572706311101 24.605281309548896 40.45254507334984 20.613600910790165 3 15.96975061996374 30.673350350756994 33.917473579540285 19.439425449738977 4 10.715714866036512 21.976954278403955 41.703600856105204 25.603729999454323 5 9.73093579049643 41.6155978724662 36.97294806519627 11.680518271841098 6 23.46179963985376 43.85826365857651 18.99074169511256 13.689195006457167 7 22.53915226182753 37.399883589093754 24.462812172215383 15.598151976863333 8 23.47392577592113 36.464503768196785 22.06683926200336 17.994731193878728 9 24.663082377514474 18.26729379095419 24.524541062948384 32.54508276858295 10-11 21.322067142415406 30.169523382221875 29.95360237187221 18.554807103490507 12-13 22.749237569194765 29.656057308119056 29.399437953593278 18.195267169092904 14-15 21.08318226188816 30.689052524358374 28.31854449988783 19.90922071386563 16-17 17.32840001697659 33.61084500979186 29.959362286504216 19.101392686727337 18-19 17.029642339616693 31.500518392316877 33.96720183347177 17.502637434594654 20-21 17.741977045224424 30.818044296775053 33.930671848568814 17.509306809431706 22-23 18.08976978530676 30.651992021002467 33.712704552757785 17.545533640932984 24-25 17.82407688110107 32.00729097192748 33.579094161159276 16.589537985812164 26-27 17.303137267088747 31.347809840275353 34.347204231228964 17.00184866140694 28-29 16.60406529944068 30.684295090415777 35.17992208933958 17.531717520803966 30-31 17.55523454978688 30.518074005808423 34.624362619972956 17.30232882443174 32-33 17.22238516729912 31.358060059281694 34.23347225348494 17.186082519934246 34-35 17.69340148924301 31.14176307938587 34.279800019403815 16.885035411967305 36-37 18.066501800367273 30.46065301302888 33.55572347025623 17.91712171634761 38-39 16.835823565438325 31.600984773605155 33.75070492568719 17.812486735269328 40-41 16.77795118741809 30.905312019865217 34.79399162125718 17.52274517145952 42-43 17.115229824510873 30.636851048735863 34.10350781189603 18.14441131485723 44-45 17.056449106979098 30.589546888334112 33.87476941219626 18.479234592490528 46-47 18.17169699211195 30.918918591185513 32.752920883025226 18.15646353367731 48-49 17.412676662644834 30.861774174968016 32.71957400883995 19.005975153547197 50-51 16.727322609787006 30.75279052706251 33.046524952556496 19.473361910593997 52-53 17.294749989389633 31.65414137862041 32.58254867127864 18.46855996071132 54-55 17.658382494709972 31.03070640805661 32.12516597648742 19.185745120746 56-57 17.700596605894514 31.34045339622756 31.7026459228899 19.256304074988027 58-59 17.34727131623144 30.692463000127322 32.543062940709255 19.417202742931977 60-61 17.786464806921597 31.95100434721978 31.48255655326708 18.779974292591536 62-63 17.107325398798302 31.394111548325682 32.289626696901166 19.208936355974853 64-65 17.23919712853098 31.281111117847853 31.801170778437303 19.678520975183865 66-67 17.22330768146411 31.23106235723348 30.964817478639024 20.580812482663386 68-69 17.221718647685105 30.5650207358184 31.458267649698058 20.75499296679844 70-71 17.631629207011333 30.858818429301593 31.506202518598457 20.00334984508861 72-73 17.701457109033832 29.884149751944356 31.697009841890107 20.717383297131708 74-75 16.915656660583387 31.086031903863994 30.827214687176003 21.171096748376613 76-77 16.836230469342098 30.60720110590361 31.982456512644532 20.574111912109768 78-79 17.093228765619976 30.060767099367624 31.494152170881513 21.351851964130887 80-81 17.190705612813698 30.135668828909946 30.96744660517195 21.70617895310441 82-83 17.21896666191722 30.194126046267694 31.163467863931388 21.423439427883697 84-85 16.686953635055882 29.68663076151166 31.23505951938833 22.391356084044133 86-87 16.397963963117295 29.970077897509977 31.520221672151376 22.111736467221352 88-89 17.226843627412343 29.64461326720547 30.85078486415696 22.277758241225225 90-91 17.01693887604684 29.146614119519498 31.61650687786578 22.219940126567888 92-93 17.242257415177164 29.335498872262505 31.2109687628841 22.21127494967623 94-95 16.861164836630632 28.397440172676173 31.534698938356787 23.206696052336405 96-97 17.30680033710658 28.445868928595246 31.0288874876465 23.21844324665167 98-99 16.80492988061819 28.581075345746456 30.639865884935098 23.97412888870026 100-101 16.399007475762886 28.401381166898076 31.52939375382731 23.670217603511727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2414.0 1 1517.0 2 679.5 3 725.0 4 627.5 5 648.0 6 1055.5 7 1999.5 8 2855.5 9 3043.5 10 2458.5 11 1733.0 12 1533.5 13 1469.5 14 1464.0 15 1440.5 16 1400.0 17 1434.5 18 1539.0 19 1704.5 20 1860.5 21 2037.0 22 2212.5 23 2444.0 24 2695.0 25 3348.0 26 5088.0 27 7154.0 28 7830.0 29 8837.0 30 11592.5 31 14214.0 32 17849.5 33 23217.5 34 29141.0 35 33288.5 36 38321.5 37 43573.5 38 42804.0 39 39784.0 40 36953.5 41 33453.5 42 30340.0 43 28606.0 44 27381.5 45 26100.0 46 22927.5 47 18949.0 48 17004.5 49 14395.5 50 10501.5 51 6594.5 52 3793.5 53 2331.5 54 1661.0 55 1335.0 56 1099.0 57 962.5 58 1024.5 59 1099.0 60 1129.5 61 1129.0 62 942.5 63 612.0 64 346.5 65 222.5 66 156.0 67 131.5 68 107.0 69 88.0 70 81.0 71 72.0 72 67.5 73 60.0 74 36.5 75 40.0 76 46.0 77 35.0 78 26.0 79 23.0 80 18.5 81 11.5 82 7.0 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027435382852430988 2 0.012884019571583614 3 0.0024252272134745625 4 0.0 5 1.5157670084216016E-4 6 0.0 7 0.0 8 0.0 9 1.5157670084216016E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001818920410105922 26-27 0.00992827390516149 28-29 3.031534016843203E-4 30-31 0.0 32-33 7.578835042108008E-5 34-35 0.01030721565726689 36-37 8.336718546318807E-4 38-39 0.013338749674110091 40-41 0.013111384622846854 42-43 0.005002031127791285 44-45 7.578835042108008E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5157670084216016E-4 68-69 6.063068033686406E-4 70-71 0.0 72-73 1.5157670084216016E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.578835042108008E-5 82-83 9.09460205052961E-4 84-85 0.004698877726106964 86-87 0.002879957316001043 88-89 0.0 90-91 0.001061036905895121 92-93 6.063068033686406E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 659732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.1210248659117 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7853211520839 14.108509604882766 2 9.153929087001448 3.5006501138669406 3 3.713050156013404 2.1299197308452884 4 2.0796795668251074 1.5906241884156538 5 1.3686168350189125 1.3084678267150998 6 1.0418505640671152 1.1952750325254864 7 0.7718457264533132 1.0330936929713037 8 0.6179052677258825 0.945198559117154 9 0.5219169019026396 0.8981627453297971 >10 5.124741813744901 21.218138788124573 >50 0.9819577292266034 13.248785051003074 >100 0.7860420918122488 28.655904504460754 >500 0.042831758786943924 5.236816006068102 >1k 0.008724987901044133 3.046025447185605 >5k 0.0015863614365534788 1.884428708488406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6616 1.0028314527717317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5809 0.8805090551921083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4361 0.6610259923726605 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4247 0.6437462484766542 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1984 0.3007281744708457 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1566 0.2373691135188228 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1269 0.19235083336870124 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1259 0.19083506636027964 TruSeq Adapter, Index 11 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.18083100410469705 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.17082694184911448 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1052 0.1594586892859525 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1020 0.15460823485900335 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1006 0.1524861610472131 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 895 0.13566114725373332 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 859 0.13020438602341558 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 854 0.12944650251920478 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 846 0.12823388891246748 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 822 0.12459604809225565 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 820 0.12429289469057132 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 811 0.12292870438299187 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 802 0.12156451407541245 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 798 0.1209582072720438 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 795 0.12050347716951733 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 768 0.11641090624677901 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 766 0.11610775284509468 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 748 0.11337937222993578 RNA PCR Primer, Index 11 (95% over 23bp) ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 718 0.10883207120467098 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 702 0.10640684399119643 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 700 0.1061036905895121 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.10261742647014241 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 670 0.10155638956424731 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.578835042108008E-5 0.0 14-15 0.0 0.0 0.0 1.5157670084216016E-4 0.0 16-17 0.0 0.0 0.0 1.5157670084216016E-4 0.0 18-19 0.0 0.0 0.0 2.2736505126324022E-4 0.0 20-21 0.0 0.0 0.0 9.094602050529609E-4 0.0 22-23 0.0 0.0 0.0 0.004623089375685885 0.0 24-25 0.0 0.0 0.0 0.013262961323689013 0.0 26-27 0.0 0.0 0.0 0.024631213886851022 0.0 28-29 0.0 0.0 0.0 0.05055082973086041 0.0 30-31 0.0 0.0 0.0 0.11481935088793632 0.0 32-33 0.0 0.0 0.0 0.21516312684544633 0.0 34-35 0.0 0.0 0.0 0.3593125693463406 0.0 36-37 0.0 0.0 0.0 0.557423317347044 0.0 38-39 0.0 0.0 0.0 0.8179836660947173 0.0 40-41 0.0 0.0 0.0 1.078013496389443 0.0 42-43 0.0 0.0 0.0 1.338194903385011 0.0 44-45 0.0 0.0 0.0 1.6551417848459677 0.0 46-47 0.0 0.0 0.0 2.0443149642582137 0.0 48-49 0.0 0.0 0.0 2.4343218155250916 0.0 50-51 0.0 0.0 0.0 2.8178108686557573 0.0 52-53 0.0 0.0 0.0 3.1831107176853632 0.0 54-55 0.0 0.0 0.0 3.592746751711301 0.0 56-57 0.0 0.0 0.0 4.079914268218004 0.0 58-59 0.0 0.0 0.0 4.581936301407238 0.0 60-61 0.0 0.0 0.0 5.08592883170742 0.0 62-63 0.0 0.0 0.0 5.570443755949386 0.0 64-65 0.0 0.0 0.0 6.111117847853371 0.0 66-67 0.0 0.0 0.0 6.718409899777486 0.0 68-69 0.0 0.0 0.0 7.382467426166989 0.0 70-71 0.0 0.0 0.0 8.10306306197062 0.0 72-73 0.0 0.0 0.0 8.765074302898753 0.0 74-75 0.0 0.0 0.0 9.475438511395536 0.0 76-77 0.0 0.0 0.0 10.241128215699709 0.0 78-79 0.0 0.0 0.0 11.085107285988855 0.0 80-81 0.0 0.0 0.0 11.988580211358553 0.0 82-83 0.0 0.0 0.0 12.850596909047916 0.0 84-85 0.0 0.0 0.0 13.695333862841274 0.0 86-87 0.0 0.0 0.0 14.57008300340138 0.0 88-89 0.0 0.0 0.0 15.470827548155917 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTATT 50 4.2249667E-7 57.027714 1 AGACCGA 80 5.2750693E-11 32.652317 94-95 AGGGTCT 175 3.6379788E-12 32.567505 5 TAGGGTC 175 3.6379788E-12 32.567505 4 GCCTATC 135 1.196895E-8 31.662851 7 GGTATCA 4600 0.0 31.613186 1 GACCGTC 40 4.497169E-4 29.683924 94-95 CCTTTAG 245 0.0 29.095772 1 CTTTAGG 235 0.0 28.303045 2 GACATAT 270 0.0 28.161833 1 GGATATG 390 0.0 28.026442 1 GCCCAAC 35 0.008392289 27.142673 90-91 CTCCGAC 280 0.0 27.139587 94-95 CACTGAC 270 0.0 26.38571 7 GTGCTTA 145 7.887302E-7 26.20374 9 CATTCCG 255 0.0 26.07529 9 TTAGGCA 335 0.0 25.519314 4 CATATAG 300 0.0 25.34565 1 GTGGTAC 150 1.0544863E-6 25.34565 1 GTACATG 3540 0.0 25.104008 1 >>END_MODULE