FastQCFastQC Report
Thu 26 May 2016
SRR938343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964568
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88680.29913295967574366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67190.2266434772283854No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59700.2013784133135081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.1634302198499073No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32630.11006662690820383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA165600.026.2514021
GTACATG120950.025.4428061
TACATGG120100.024.988392
ACATGGG126950.023.1155593
GGTATCA120300.022.7336311
ATCAACG192650.022.4659253
TATCAAC193050.022.394742
TCAACGC193400.022.3296184
CAACGCA195350.022.1067225
GAGTACT88400.021.74698612-13
AACGCAG200050.021.5954276
AGTACTT90350.021.40913412-13
AGAGTAC153350.020.31625710-11
GTACTTT94700.020.09271814-15
GGACCGA8250.019.5867066
ACGCAGA220650.019.5819117
CATGGGG61500.019.3337824
CGCAGAG223200.019.2510628
GTGTAGC18500.018.767991
GCAGAGT226450.018.1969229
Produced by FastQC (version 0.11.5)