FastQCFastQC Report
Thu 26 May 2016
SRR938343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964568
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90260.30446257262440934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73000.24624161091936497No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63440.21399407940718512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52290.17638320321881637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32290.108919748172415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164600.023.9076181
GGACCGA8700.022.9209966
GTACATG121450.022.892551
TACATGG118550.022.890912
GGTATCA122450.022.7444081
TAAGGTG13900.021.870155
ACATGGG123500.021.384273
AGGACCG10500.020.3568135
GAGTACT93300.020.15194512-13
GTAAGGT13350.019.9247724
ATCAACG194800.019.8238563
TATCAAC196000.019.8104552
TCAACGC196350.019.7157484
AGTACTT97750.019.64740412-13
CAACGCA197900.019.5133275
GTACTTT97800.019.20043614-15
AACGCAG202250.019.1090586
AGAGTAC162350.019.09696210-11
ACCGAGT10050.018.4247678
GTGTAGC17100.018.3435611
Produced by FastQC (version 0.11.5)