##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2964568 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801180475536402 31.0 31.0 34.0 30.0 34.0 2 32.01939034624944 31.0 31.0 34.0 30.0 34.0 3 32.08612350939496 33.0 31.0 34.0 30.0 34.0 4 35.74956587266678 37.0 35.0 37.0 33.0 37.0 5 35.64780433439206 37.0 35.0 37.0 33.0 37.0 6 35.6500114013239 37.0 35.0 37.0 33.0 37.0 7 35.59104024599874 37.0 35.0 37.0 33.0 37.0 8 35.57403911800977 37.0 35.0 37.0 33.0 37.0 9 37.156251770915695 39.0 37.0 39.0 33.0 39.0 10-11 37.0560663476095 39.0 37.0 39.0 33.0 39.0 12-13 36.94743702961106 39.0 37.0 39.0 33.0 39.0 14-15 37.97977614276347 40.0 37.0 41.0 33.0 41.0 16-17 37.89577941878885 40.0 37.0 41.0 33.0 41.0 18-19 38.14369952046976 40.0 37.0 41.0 33.0 41.0 20-21 38.11812041417164 40.0 37.0 41.0 33.0 41.0 22-23 38.00792020962245 40.0 37.0 41.0 33.0 41.0 24-25 37.88767081746818 40.0 37.0 41.0 32.0 41.0 26-27 37.69496348203178 40.0 37.0 41.0 32.0 41.0 28-29 37.583903793065296 39.5 37.0 41.0 32.0 41.0 30-31 37.348971249773996 39.0 36.0 41.0 31.5 41.0 32-33 37.15835224558856 39.0 36.0 41.0 31.0 41.0 34-35 37.05772173213771 39.0 36.0 41.0 31.0 41.0 36-37 37.02378677095618 39.0 36.0 41.0 31.0 41.0 38-39 36.86635793140856 39.0 35.5 41.0 30.0 41.0 40-41 36.707471543914664 39.0 35.0 41.0 30.0 41.0 42-43 36.501599389860516 39.0 35.0 40.0 30.0 41.0 44-45 36.20424864600846 39.0 35.0 40.0 30.0 41.0 46-47 35.82978008937559 38.0 35.0 40.0 28.5 41.0 48-49 35.4848814397241 38.0 34.5 40.0 27.5 41.0 50-51 35.116169371051704 38.0 34.0 40.0 26.5 41.0 52-53 34.637790059124974 38.0 33.0 40.0 25.5 41.0 54-55 34.30702365403661 38.0 33.0 40.0 24.0 41.0 56-57 33.97462092284609 37.0 33.0 40.0 24.0 41.0 58-59 33.50195778946545 37.0 32.0 39.5 23.0 41.0 60-61 33.09575492955466 36.0 31.5 39.0 22.0 40.0 62-63 32.62338121439616 36.0 31.0 39.0 20.5 40.0 64-65 32.1049255743164 35.0 31.0 38.5 19.0 40.0 66-67 31.58691873487132 35.0 30.0 38.0 17.5 40.0 68-69 31.084097750498557 34.5 29.0 38.0 13.5 40.0 70-71 30.490493218573498 34.0 29.0 37.0 9.5 39.0 72-73 29.977047751982752 34.0 28.5 36.5 8.0 39.0 74-75 29.373882467867155 34.0 28.0 36.0 7.0 38.5 76-77 28.14188913865359 31.5 26.5 34.5 4.0 36.5 78-79 28.82643676920212 33.0 28.0 35.0 2.0 37.0 80-81 28.424047955722386 33.0 27.0 35.0 2.0 37.0 82-83 27.958096424167028 33.0 26.0 35.0 2.0 36.0 84-85 27.38066456900297 32.5 26.0 35.0 2.0 36.0 86-87 26.752716247358805 32.0 25.0 34.5 2.0 35.0 88-89 25.98747810810884 31.5 23.5 34.0 2.0 35.0 90-91 25.346757773813927 31.0 21.0 34.0 2.0 35.0 92-93 24.49281497337892 31.0 18.0 34.0 2.0 35.0 94-95 23.647131723745247 30.5 8.0 34.0 2.0 35.0 96-97 22.54097612198472 30.0 2.0 34.0 2.0 35.0 98-99 21.123771996459517 29.0 2.0 34.0 2.0 35.0 100-101 17.155491963753235 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3322222857192081 1101 2 0.3471304125671111 1101 3 0.3955934862641115 1101 4 0.26413052844520735 1101 5 0.1107410063073857 1101 6 0.24216245450411833 1101 7 0.23904280330356187 1101 8 0.28147325617077 1101 9 0.31898468366328814 1101 10-11 0.3304021048323662 1101 12-13 0.4013402690604835 1101 14-15 0.4907438401604196 1101 16-17 0.5125476127901862 1101 18-19 0.5182354134105438 1101 20-21 0.40599342210419564 1101 22-23 0.39098559652028086 1101 24-25 0.5386211705793826 1101 26-27 0.40514617589340673 1101 28-29 0.31481435777944 1101 30-31 0.4900106568990594 1101 32-33 0.3945739137134794 1101 34-35 0.4025939471714324 1101 36-37 0.5717364065763988 1101 38-39 0.6443903138902272 1101 40-41 0.6527853039192024 1101 42-43 0.6295152917514102 1101 44-45 0.7294342189514111 1101 46-47 0.7078026503383086 1101 48-49 0.5773058100496158 1101 50-51 0.6437361727704811 1101 52-53 0.6669580922817602 1101 54-55 0.6982415710714847 1101 56-57 0.7497994805058283 1101 58-59 0.952481127153348 1101 60-61 0.757571993604671 1101 62-63 0.7455372266611064 1101 64-65 0.7050901968535825 1101 66-67 0.5900079124904103 1101 68-69 0.7850238421574609 1101 70-71 0.8605931325737863 1101 72-73 0.8847808677456861 1101 74-75 0.8228029422521494 1101 76-77 0.91217973966112 1101 78-79 1.0924574904645148 1101 80-81 1.0521025448347032 1101 82-83 1.0682297503472284 1101 84-85 1.2288408878021393 1101 86-87 1.1821806003320035 1101 88-89 1.189182429015677 1101 90-91 1.3996357321581172 1101 92-93 1.7590054635323398 1101 94-95 1.8056224225078203 1101 96-97 1.8202421984754622 1101 98-99 1.870444643856544 1101 100-101 2.0244891899971655 1102 1 0.3319967977815885 1102 2 0.33167973531104167 1102 3 0.20904097743655825 1102 4 0.19486343737367662 1102 5 0.1312441978758443 1102 6 0.1317106619969053 1102 7 0.19001063409741192 1102 8 0.21876481694952332 1102 9 0.2043694087447392 1102 10-11 0.3597834878169337 1102 12-13 0.39500271727443703 1102 14-15 0.4540400715866042 1102 16-17 0.452666243822442 1102 18-19 0.383576515717472 1102 20-21 0.3984881475147404 1102 22-23 0.3831539129054562 1102 24-25 0.5046945601993755 1102 26-27 0.511133185350495 1102 28-29 0.4666553753760212 1102 30-31 0.5307807652429588 1102 32-33 0.491835332239809 1102 34-35 0.5044740899723337 1102 36-37 0.663351485259497 1102 38-39 0.6504832417353086 1102 40-41 0.5845632580244882 1102 42-43 0.4698310114296831 1102 44-45 0.6409533616584611 1102 46-47 0.5811801427418075 1102 48-49 0.5035195750521808 1102 50-51 0.5659813500710413 1102 52-53 0.5245804987134122 1102 54-55 0.4896188513944324 1102 56-57 0.553630337737836 1102 58-59 0.6290081375611365 1102 60-61 0.575885880126954 1102 62-63 0.623311916676137 1102 64-65 0.6687980961226359 1102 66-67 0.6637060853068029 1102 68-69 0.7460992611688972 1102 70-71 0.8524615500019301 1102 72-73 0.8372058381580132 1102 74-75 0.9262329184966909 1102 76-77 0.7261272655709021 1102 78-79 0.9274356668887158 1102 80-81 0.8841008067493057 1102 82-83 0.9956436596620755 1102 84-85 0.8504517126857678 1102 86-87 0.747522251123101 1102 88-89 0.7822966368277982 1102 90-91 0.9759936875553841 1102 92-93 1.3580092334468894 1102 94-95 1.2369730464999797 1102 96-97 1.2792802425565242 1102 98-99 1.3196359953581087 1102 100-101 1.5468845910183227 1103 1 0.21277656766364927 1103 2 0.166637120225694 1103 3 0.2152779405269385 1103 4 0.1871427728386479 1103 5 0.07420329935356307 1103 6 0.151008629909839 1103 7 0.21713373769973288 1103 8 0.27282811169536103 1103 9 0.30074457626792395 1103 10-11 0.3270500721729874 1103 12-13 0.32306025731291754 1103 14-15 0.4625236921276752 1103 16-17 0.5081713812002562 1103 18-19 0.5267559915325748 1103 20-21 0.4524664934936595 1103 22-23 0.39396632562630884 1103 24-25 0.4461319707217726 1103 26-27 0.5406994014193529 1103 28-29 0.45426674971692194 1103 30-31 0.3897318678181634 1103 32-33 0.3578419311585179 1103 34-35 0.5188483710736094 1103 36-37 0.5454227226271087 1103 38-39 0.46360932118262355 1103 40-41 0.5414544849098064 1103 42-43 0.5993463989402841 1103 44-45 0.668328673401092 1103 46-47 0.5210332499671964 1103 48-49 0.6864204259468707 1103 50-51 0.7134710524513608 1103 52-53 0.6865427976416143 1103 54-55 0.5711710746601923 1103 56-57 0.6679525591392945 1103 58-59 0.7783779689650956 1103 60-61 0.8361731230382148 1103 62-63 0.7260118100944553 1103 64-65 0.8023665774919451 1103 66-67 0.6835100523966311 1103 68-69 0.8336816157446982 1103 70-71 0.7246567457358495 1103 72-73 0.7204111327701845 1103 74-75 0.836862770513477 1103 76-77 0.7755306895514273 1103 78-79 1.1319432278566026 1103 80-81 1.2500542907040106 1103 82-83 1.3430630110967066 1103 84-85 1.2890397340302435 1103 86-87 1.3418240003993631 1103 88-89 1.4802291984112586 1103 90-91 1.5049121472917797 1103 92-93 1.5706727554262834 1103 94-95 1.3908734788065438 1103 96-97 1.5943816006815297 1103 98-99 1.9621794986241525 1103 100-101 2.0813180372649214 1104 1 0.16265053673061658 1104 2 0.18540329419543156 1104 3 0.1538057647961253 1104 4 0.11465515747833166 1104 5 -0.025189735672952906 1104 6 0.02864973414246208 1104 7 0.07016419349479719 1104 8 0.08417291216319 1104 9 0.12862597947952992 1104 10-11 0.20481418259625173 1104 12-13 0.22701762861012753 1104 14-15 0.24021117574365292 1104 16-17 0.3012648860700935 1104 18-19 0.2895812353604157 1104 20-21 0.27574323053600125 1104 22-23 0.24300445754541045 1104 24-25 0.32494028943551 1104 26-27 0.21206685384687063 1104 28-29 0.23884795732852382 1104 30-31 0.21130197798600392 1104 32-33 0.3141707004916654 1104 34-35 0.4155806410419487 1104 36-37 0.4035317208996432 1104 38-39 0.2956225225027609 1104 40-41 0.43427341038687217 1104 42-43 0.5972015205013221 1104 44-45 0.4229887931808918 1104 46-47 0.4893984775286526 1104 48-49 0.6338372959434437 1104 50-51 0.6218774837258607 1104 52-53 0.5608422336951975 1104 54-55 0.5695330086132557 1104 56-57 0.6689127468077629 1104 58-59 0.6387141858861796 1104 60-61 0.7088849054180812 1104 62-63 0.7936269015424955 1104 64-65 0.5457282761872762 1104 66-67 0.4744304478985093 1104 68-69 0.7249925390977232 1104 70-71 0.8047119304803942 1104 72-73 0.6674431883179004 1104 74-75 0.7525423629126315 1104 76-77 0.7109440374035252 1104 78-79 0.9352606980964993 1104 80-81 0.8966702761314771 1104 82-83 0.9596580921934112 1104 84-85 1.1044531490988376 1104 86-87 1.2048428277403431 1104 88-89 1.0952440196489839 1104 90-91 1.201245487346764 1104 92-93 1.4787004217367006 1104 94-95 1.4713853996523945 1104 96-97 1.341636815001337 1104 98-99 1.3271489487259842 1104 100-101 1.5552585372212313 1105 1 0.15050296865127777 1105 2 0.1440421888790624 1105 3 0.10297479839316281 1105 4 0.12594956082466524 1105 5 0.09650257942565332 1105 6 0.10170363596699872 1105 7 0.13121815950728433 1105 8 0.11641879477414108 1105 9 0.1563701477375261 1105 10-11 0.20023410546374265 1105 12-13 0.234275145285622 1105 14-15 0.46466785155710255 1105 16-17 0.3235846383046308 1105 18-19 0.3162601512260679 1105 20-21 0.262341323084307 1105 22-23 0.18755623849336445 1105 24-25 0.21868811640622 1105 26-27 0.09558606476310416 1105 28-29 0.18593328984880486 1105 30-31 0.31680288464068695 1105 32-33 0.33552576144345636 1105 34-35 0.3966141738928073 1105 36-37 0.4654746899129094 1105 38-39 0.41779624716858166 1105 40-41 0.5033594275785447 1105 42-43 0.6291976050368362 1105 44-45 0.6370162656201899 1105 46-47 0.6938370737383508 1105 48-49 0.6956473424262057 1105 50-51 0.6490041104890167 1105 52-53 0.7438214635468725 1105 54-55 0.5705578760919039 1105 56-57 0.5077646384799124 1105 58-59 0.5077590158148766 1105 60-61 0.5885638052367526 1105 62-63 0.7314584723819095 1105 64-65 0.6770487826301377 1105 66-67 0.5412894109422943 1105 68-69 0.6168390657276959 1105 70-71 0.7478887522144326 1105 72-73 0.7299908612375425 1105 74-75 0.6940433444532523 1105 76-77 0.4394210180027187 1105 78-79 0.7689684610932197 1105 80-81 0.7375523789284664 1105 82-83 0.8932380142050604 1105 84-85 0.9263773431389879 1105 86-87 0.8812144438322633 1105 88-89 1.0381314607431733 1105 90-91 1.0845475290582272 1105 92-93 1.1469327392958455 1105 94-95 1.3700631280411173 1105 96-97 1.4161940519243785 1105 98-99 1.4722867120971337 1105 100-101 1.4384648319187754 1106 1 -0.049587191321322166 1106 2 0.1107860694709828 1106 3 0.09093241360320548 1106 4 0.0389459341531051 1106 5 -0.03375777483368125 1106 6 -0.0952716836741061 1106 7 -0.028035507976333918 1106 8 0.07123275047091226 1106 9 -0.16906552765669858 1106 10-11 -0.008056344087755463 1106 12-13 0.06030576980080582 1106 14-15 0.05541909219033414 1106 16-17 0.11931414985097177 1106 18-19 0.06571945813595192 1106 20-21 0.039156606106601544 1106 22-23 0.060938133730672916 1106 24-25 0.05786982662886686 1106 26-27 0.03451735432812342 1106 28-29 0.18215734751704815 1106 30-31 0.03500634315589224 1106 32-33 0.02816940121756062 1106 34-35 0.009957369695719365 1106 36-37 0.05956291651426682 1106 38-39 -0.09585962768723988 1106 40-41 -0.023123543923439627 1106 42-43 0.04512876549704714 1106 44-45 -0.14728053460771662 1106 46-47 -0.7250814760624991 1106 48-49 -0.46211116328694146 1106 50-51 -0.11822674065313521 1106 52-53 -0.1598955280335801 1106 54-55 0.058331223523630626 1106 56-57 -0.08853016008990977 1106 58-59 -0.1950877986572408 1106 60-61 -0.09902415408563314 1106 62-63 -0.27329701270540596 1106 64-65 -0.22905483411848948 1106 66-67 -0.22931256958954904 1106 68-69 -0.4432085963548609 1106 70-71 -0.22226093467972063 1106 72-73 0.1482719426877388 1106 74-75 0.0642321362349314 1106 76-77 0.12949877964535972 1106 78-79 0.3901048395218325 1106 80-81 -0.099765954061656 1106 82-83 0.21262806331823825 1106 84-85 0.4772378107118094 1106 86-87 0.677599135629201 1106 88-89 0.6804374007382457 1106 90-91 0.5407983893124033 1106 92-93 0.5959783773136955 1106 94-95 0.9866817449551313 1106 96-97 1.3402547610619067 1106 98-99 1.505687460905051 1106 100-101 1.7033346121098276 1107 1 0.1262223656116177 1107 2 0.1509272994261437 1107 3 0.16177014613992213 1107 4 0.11958074437664123 1107 5 0.03980808486382159 1107 6 0.10958565792351749 1107 7 0.1252112076301799 1107 8 0.09844839631537639 1107 9 0.1739868363199335 1107 10-11 0.11888261774165443 1107 12-13 0.16661691872781148 1107 14-15 0.3282319117795254 1107 16-17 0.2134916747867024 1107 18-19 0.1877411456910707 1107 20-21 0.21554178586323047 1107 22-23 0.2993871712884797 1107 24-25 0.2664886423840116 1107 26-27 0.30226705311208235 1107 28-29 0.25689391286747565 1107 30-31 0.20202836194515328 1107 32-33 0.21068044580581358 1107 34-35 0.2981464919666621 1107 36-37 0.40425561404367727 1107 38-39 0.43011875514489617 1107 40-41 0.48298441356266153 1107 42-43 0.38868915983128716 1107 44-45 0.3783845214329631 1107 46-47 0.33246000066209547 1107 48-49 0.39308600383309766 1107 50-51 0.40133915630821093 1107 52-53 0.3635074058727952 1107 54-55 0.4120387686232263 1107 56-57 0.418176399588134 1107 58-59 0.3368445177872914 1107 60-61 0.4194132695752515 1107 62-63 0.568106943886356 1107 64-65 0.4105999992086353 1107 66-67 0.4274692423439852 1107 68-69 0.5612245585260816 1107 70-71 0.763731794730969 1107 72-73 0.8102758678122619 1107 74-75 0.7662620839413421 1107 76-77 0.777940737073493 1107 78-79 1.0745875553105186 1107 80-81 0.9705307282144204 1107 82-83 1.0762624884820973 1107 84-85 1.2305133022839705 1107 86-87 1.4019752768343139 1107 88-89 1.3684210543335844 1107 90-91 1.4838813924467082 1107 92-93 1.6738090835462778 1107 94-95 1.7412574340256448 1107 96-97 1.8785942843331753 1107 98-99 1.8831237914932792 1107 100-101 1.7617548944041204 1108 1 0.22894897666362723 1108 2 0.1578290530917954 1108 3 0.19740831496211797 1108 4 0.16192626441098668 1108 5 0.07540830632709117 1108 6 0.16809830807996917 1108 7 0.1661091292346626 1108 8 0.15374360810518084 1108 9 0.12008821175616902 1108 10-11 0.17420908944598779 1108 12-13 0.30118462987998385 1108 14-15 0.36773775629488625 1108 16-17 0.36022882400691003 1108 18-19 0.3565621025866008 1108 20-21 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-0.9412095480950846 2316 76-77 -1.0334569069889064 2316 78-79 -1.3176192890426144 2316 80-81 -1.330197467949521 2316 82-83 -1.2768100818446264 2316 84-85 -1.4756886043315198 2316 86-87 -1.52335135270922 2316 88-89 -1.4459752742001726 2316 90-91 -1.3444664477435957 2316 92-93 -1.711038609473011 2316 94-95 -1.5223386145828393 2316 96-97 -1.8614455419449598 2316 98-99 -1.9762416911976075 2316 100-101 -2.357929228546748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 19.0 4 53.0 5 93.0 6 223.0 7 536.0 8 1227.0 9 2213.0 10 3687.0 11 5793.0 12 8140.0 13 11425.0 14 15053.0 15 19858.0 16 25083.0 17 30980.0 18 30063.0 19 26026.0 20 26794.0 21 28658.0 22 31261.0 23 35499.0 24 40690.0 25 47926.0 26 56921.0 27 67863.0 28 80393.0 29 96026.0 30 113162.0 31 134560.0 32 160705.0 33 196760.0 34 247254.0 35 320629.0 36 416511.0 37 461828.0 38 213293.0 39 7361.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.13744871486528 19.51842318217957 15.134659424203246 23.209468678751907 2 15.741937269304199 20.929300142724703 39.138629930608595 24.190132657362508 3 19.67372649235909 24.109516125115025 29.71893375358568 26.497823628940203 4 13.302684675367432 17.119869444677754 36.5348126724746 33.042633207480215 5 13.804865158744864 37.41820784698451 34.02850546597186 14.74842152829876 6 32.292551422366714 36.25103978120238 17.055953552729132 14.400455243701776 7 28.950727390972308 32.21359739429151 20.27583782864822 18.559837386087956 8 26.30400112259189 35.2952942890836 19.432443445385637 18.968261142938868 9 26.15369254474851 16.909613812197932 20.113588219261626 36.82310542379193 10-11 25.14447298898187 26.686957425162788 27.031931802542562 21.13663778331278 12-13 26.118763340898237 23.822307331118733 27.451470163612374 22.60745916437066 14-15 22.65087189769302 25.454484430783847 26.006015041651935 25.888628629871196 16-17 22.226003923674543 28.617812106182082 26.621551605495302 22.53463236464807 18-19 22.239277358454927 27.27447978929814 28.755555615523072 21.730687236723867 20-21 23.035683445277694 26.211323201221898 28.797703409063306 21.955289944437098 22-23 22.54686011587523 26.218592388503147 28.70539653669607 22.529150958925552 24-25 22.5991203916492 26.36912346985676 28.79667384915096 22.235082289343076 26-27 22.268705452165786 26.542986867981554 28.89605702122779 22.292250658624866 28-29 22.06003415343748 27.06365610439989 28.77002930449265 22.106280437669977 30-31 22.288266277216255 26.570444215327306 28.65321007333566 22.488079434120774 32-33 22.176508050446678 26.581130530170455 28.62440446393996 22.617956955442907 34-35 22.702369723246658 26.552630229523505 28.504639319242646 22.240360727987184 36-37 22.653440519535817 26.28807239437946 28.6384739715032 22.42001311458153 38-39 22.247458413274018 26.67372815247412 28.630423140983748 22.448390293268115 40-41 22.608014410354407 26.530366482433166 28.483185389951544 22.378433717260886 42-43 22.333696123875683 26.5282867836468 28.30208123033725 22.835935862140268 44-45 22.452147890465778 26.56702702529471 28.290932594725493 22.689892489514023 46-47 22.714489935801776 26.905808873333314 27.85235487936185 22.527346311503056 48-49 22.570657849642846 26.770359121463905 27.910845694887083 22.74813733400617 50-51 22.484296539090963 27.04579289933543 27.883102801146954 22.586807760426655 52-53 22.899879510269287 27.082849845238833 27.395863410790376 22.621407233701504 54-55 22.433360161753107 26.872074988244293 27.70261472522683 22.991950124775766 56-57 22.58160379522413 26.9001925407007 27.642678461077637 22.875525202997537 58-59 22.873999927798103 26.70405757797721 27.462464291269207 22.959478202955484 60-61 22.432121644705063 26.916923477552213 27.913038257176087 22.73791662056664 62-63 22.37674892541522 26.96654116701686 27.815531876241973 22.84117803132594 64-65 22.1301752590223 27.078529361258813 27.686111290103295 23.105184089615594 66-67 22.242693070856685 26.906723771394876 27.52400334910055 23.326579808647885 68-69 22.39445819048435 26.732996276086457 27.663603552663453 23.20894198076574 70-71 22.400837492037198 26.851000593510243 27.444342181004266 23.303819733448293 72-73 22.454729476513773 26.693712774917888 27.564784334440745 23.286773414127595 74-75 22.201429465037016 27.212114689197296 27.274161191346824 23.312294654418864 76-77 22.291079973818082 26.77877509585779 27.486864745478023 23.443280184846106 78-79 22.348186156462962 26.937988223420955 27.486917442813475 23.2269081773026 80-81 22.371596873676214 27.044785909173036 27.3471510649924 23.236466152158354 82-83 22.283832826332333 26.996343856345778 27.438421733724088 23.2814015835978 84-85 22.3171445941086 26.85502061658814 27.408844722106206 23.418990067197054 86-87 22.237736617962184 27.009414115843622 27.25637188490125 23.496477381292948 88-89 22.15112258338078 26.985973045980437 27.044191513607572 23.81871285703121 90-91 22.159880439636375 26.849594877758165 27.23991378130906 23.7506109012964 92-93 22.303792108412953 26.738054609667657 27.293446620572382 23.66470666134701 94-95 22.006365155455384 26.58428348138407 27.39287585078915 24.016475512371397 96-97 21.912746791709882 26.599235250875203 27.45765880405493 24.03035915335999 98-99 21.96976651414138 26.732514210231095 27.001194057656313 24.296525217971208 100-101 21.908404492228946 26.464226904185733 27.419225333747622 24.208143269837702 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 616.0 1 425.5 2 301.5 3 425.0 4 407.0 5 397.0 6 688.0 7 1493.0 8 2318.0 9 2556.5 10 2247.0 11 1925.5 12 1983.0 13 2148.0 14 2375.5 15 2594.0 16 2763.5 17 2963.0 18 3225.5 19 3588.0 20 4034.5 21 4721.5 22 5656.5 23 6816.0 24 8178.0 25 10030.0 26 12651.5 27 16463.5 28 20576.5 29 25545.5 30 31412.5 31 36660.5 32 43656.5 33 49176.5 34 52726.0 35 58226.0 36 65307.5 37 71881.5 38 76297.5 39 79785.5 40 84823.0 41 91341.0 42 96715.0 43 101739.0 44 106900.5 45 111862.5 46 115196.0 47 122326.0 48 133139.0 49 132178.0 50 122234.5 51 113337.0 52 106408.0 53 102429.5 54 99711.5 55 93405.0 56 83496.5 57 75360.0 58 69020.0 59 61207.5 60 50901.5 61 41198.5 62 33248.0 63 24892.5 64 18213.0 65 14069.5 66 10561.0 67 8000.0 68 6004.5 69 4369.5 70 3354.0 71 2582.0 72 2132.0 73 1917.5 74 1605.5 75 1316.5 76 944.5 77 570.0 78 355.0 79 241.5 80 125.0 81 68.0 82 47.5 83 28.5 84 20.0 85 10.5 86 6.5 87 6.0 88 4.0 89 5.0 90 3.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.059759128480103E-4 2 0.05130595756278823 3 0.0 4 3.3731727523200686E-5 5 0.04779785790037537 6 6.746345504640137E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006088576817937723 26-27 0.0019564401963456397 28-29 1.8552450137760375E-4 30-31 0.0016359887848752328 32-33 4.0478073027840815E-4 34-35 0.011468787357888232 36-37 0.02332548958229327 38-39 0.004401990441777689 40-41 0.0011131470082656227 42-43 0.0016022570573520324 44-45 0.008517261199608172 46-47 0.0 48-49 0.0 50-51 4.891100490864099E-4 52-53 0.0 54-55 0.001686586376160034 56-57 0.0 58-59 0.022161744982742846 60-61 0.0 62-63 0.009562944752827393 64-65 0.0148756918377315 66-67 0.006409028229408129 68-69 0.01625869266618273 70-71 8.432931880800171E-5 72-73 3.3731727523200686E-5 74-75 0.02233040362035885 76-77 0.04906279768249539 78-79 0.029987505768125405 80-81 0.02588910087405652 82-83 0.03585682635716233 84-85 0.015179277385440306 86-87 0.009984591346867402 88-89 0.08262586656808008 90-91 0.023932660677710882 92-93 0.02413505104285009 94-95 0.026158954694242127 96-97 0.037526546869560765 98-99 0.052688958391239465 100-101 0.014403447652406693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2964568.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.79008565133386 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93231912304887 37.04238735010067 2 14.473075355888444 14.701774739275669 3 5.229143840887511 7.9676599068546485 4 2.438875105183503 4.95482701940704 5 1.312631210290647 3.3334325799637994 6 0.8348508924767634 2.5441289000992398 7 0.5282602952575669 1.8781269949631516 8 0.359074247870714 1.4589929443633483 9 0.28984872357873764 1.3249297346844489 >10 1.4460342037597167 13.08869538358155 >50 0.09890534798281214 3.453490127266222 >100 0.05125836378023768 4.639032319521149 >500 0.0036602436013635814 1.2726993728424025 >1k 0.001796846858629447 1.3968157006810558 >5k 2.661995346117699E-4 0.943006926395663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9026 0.30446257262440934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7300 0.24624161091936497 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6344 0.21399407940718512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.17638320321881637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3229 0.108919748172415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.373172752320068E-5 0.0 6 0.0 0.0 0.0 3.373172752320068E-5 0.0 7 0.0 0.0 0.0 3.373172752320068E-5 0.0 8 0.0 0.0 0.0 3.373172752320068E-5 0.0 9 0.0 0.0 0.0 3.373172752320068E-5 0.0 10-11 0.0 0.0 0.0 3.373172752320068E-5 0.0 12-13 0.0 0.0 0.0 5.059759128480102E-5 0.0 14-15 0.0 0.0 0.0 8.432931880800171E-5 0.0 16-17 0.0 0.0 0.0 1.0119518256960205E-4 0.0 18-19 0.0 0.0 0.0 1.0119518256960205E-4 0.0 20-21 0.0 0.0 0.0 2.023903651392041E-4 0.0 22-23 0.0 0.0 0.0 3.8791486651680785E-4 0.0 24-25 0.0 0.0 0.0 7.926955967952161E-4 0.0 26-27 0.0 0.0 0.0 0.0015347936023056312 0.0 28-29 0.0 0.0 0.0 0.004216465940400085 0.0 30-31 0.0 0.0 0.0 0.013037312687717063 0.0 32-33 0.0 0.0 0.0 0.03126931141400703 0.0 34-35 0.0 0.0 0.0 0.058625742435322786 0.0 36-37 0.0 0.0 0.0 0.10590075855908854 0.0 38-39 0.0 0.0 0.0 0.18017802256517645 0.0 40-41 0.0 0.0 0.0 0.26138715657728206 0.0 42-43 0.0 0.0 0.0 0.34679589066602623 0.0 44-45 0.0 0.0 0.0 0.4500487086145435 0.0 46-47 0.0 0.0 0.0 0.573675489987074 0.0 48-49 0.0 0.0 0.0 0.702260835305515 0.0 50-51 0.0 0.0 0.0 0.8411849551098171 0.0 52-53 0.0 0.0 0.0 0.9769214266631766 0.0 54-55 0.0 0.0 0.0 1.1300128720272227 0.0 56-57 0.0 0.0 0.0 1.316954105960801 0.0 58-59 0.0 0.0 0.0 1.5136269432848226 0.0 60-61 0.0 0.0 0.0 1.7259175704520862 0.0 62-63 0.0 0.0 0.0 1.9420873462845178 0.0 64-65 0.0 0.0 0.0 2.164834808983973 0.0 66-67 0.0 0.0 0.0 2.4231355124928826 0.0 68-69 0.0 0.0 0.0 2.7068868044180467 0.0 70-71 0.0 0.0 0.0 3.009578461347488 0.0 72-73 0.0 0.0 0.0 3.316874499083846 0.0 74-75 0.0 0.0 0.0 3.6361958976822253 1.686586376160034E-5 76-77 0.0 0.0 0.0 3.9793487617757464 3.373172752320068E-5 78-79 1.686586376160034E-5 1.686586376160034E-5 0.0 4.368680360848528 3.373172752320068E-5 80-81 3.373172752320068E-5 3.373172752320068E-5 0.0 4.782315669601777 3.373172752320068E-5 82-83 3.373172752320068E-5 3.373172752320068E-5 0.0 5.204451373690872 3.373172752320068E-5 84-85 3.373172752320068E-5 3.373172752320068E-5 0.0 5.624849893812522 3.373172752320068E-5 86-87 3.373172752320068E-5 3.373172752320068E-5 0.0 6.070699002350427 3.373172752320068E-5 88-89 3.373172752320068E-5 3.373172752320068E-5 0.0 6.554125255349177 3.373172752320068E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16460 0.0 23.907618 1 GGACCGA 870 0.0 22.920996 6 GTACATG 12145 0.0 22.89255 1 TACATGG 11855 0.0 22.89091 2 GGTATCA 12245 0.0 22.744408 1 TAAGGTG 1390 0.0 21.87015 5 ACATGGG 12350 0.0 21.38427 3 AGGACCG 1050 0.0 20.356813 5 GAGTACT 9330 0.0 20.151945 12-13 GTAAGGT 1335 0.0 19.924772 4 ATCAACG 19480 0.0 19.823856 3 TATCAAC 19600 0.0 19.810455 2 TCAACGC 19635 0.0 19.715748 4 AGTACTT 9775 0.0 19.647404 12-13 CAACGCA 19790 0.0 19.513327 5 GTACTTT 9780 0.0 19.200436 14-15 AACGCAG 20225 0.0 19.109058 6 AGAGTAC 16235 0.0 19.096962 10-11 ACCGAGT 1005 0.0 18.424767 8 GTGTAGC 1710 0.0 18.343561 1 >>END_MODULE