FastQCFastQC Report
Thu 26 May 2016
SRR938342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931730
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88170.3007439293522937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67340.2296937303230516No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60540.20649923424053374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48750.16628407117981533No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32130.10959399398989675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168900.025.1927011
GTACATG122100.024.051181
TACATGG121850.023.7518962
GGTATCA125000.022.541281
ACATGGG126950.021.9741423
ATCAACG193600.021.7733293
TATCAAC195700.021.6611022
TCAACGC195500.021.6103384
GAGTACT90400.021.49013112-13
CAACGCA198200.021.2916075
GTACTTT92200.020.9444714-15
AACGCAG201600.020.9285936
TAAGGTG14950.020.0261965
GTAAGGT12850.019.9708774
AGAGTAC156450.019.31091110-11
AGTACTT93000.019.17834712-13
TATATCG1759.907417E-519.0089935
ACGCAGA223150.018.8194227
CGCAGAG226950.018.4624488
CATGGGG62700.018.1907354
Produced by FastQC (version 0.11.5)