FastQCFastQC Report
Thu 26 May 2016
SRR938342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931730
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.3028246120891078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72340.2467485068543147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62570.21342347351222657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52890.1804054261477012No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32980.11249330600021148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123500.024.9538081
TACATGG122300.024.498692
GGTATCA126650.024.2205091
GTATCAA166200.023.8365151
ACATGGG127700.023.0059933
TAGGACG3450.022.046364
GAGTACT92300.021.2262312-13
GTACTTT95600.020.24512314-15
ATCAACG195000.020.1611023
TCAACGC195250.020.1105984
TATCAAC196150.020.0272142
CAACGCA196400.019.9686375
AACGCAG200600.019.6042426
AGTACTT94800.019.51415612-13
GGACCGA8950.019.1042566
TAAGGTG13750.019.0149845
CATGGGG61100.018.906064
AGAGTAC161700.018.6662110-11
TAGCCCT20350.018.2207454
CATGGGA69200.017.929594
Produced by FastQC (version 0.11.5)