##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2931730 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.791040784792596 31.0 31.0 34.0 30.0 34.0 2 32.0115876973664 31.0 31.0 34.0 30.0 34.0 3 32.08339274080492 33.0 31.0 34.0 30.0 34.0 4 35.74149768225587 37.0 35.0 37.0 33.0 37.0 5 35.60985288549764 37.0 35.0 37.0 33.0 37.0 6 35.64447169418739 37.0 35.0 37.0 33.0 37.0 7 35.58704962598875 37.0 35.0 37.0 33.0 37.0 8 35.57970788578757 37.0 35.0 37.0 33.0 37.0 9 37.157052320643444 39.0 37.0 39.0 33.0 39.0 10-11 37.064644936607394 39.0 37.0 39.0 33.0 39.0 12-13 36.9588879262415 39.0 37.0 39.0 33.0 39.0 14-15 37.999856057686074 40.0 37.0 41.0 33.0 41.0 16-17 37.92978258570878 40.0 37.0 41.0 33.0 41.0 18-19 38.041475340498614 40.0 37.0 41.0 33.0 41.0 20-21 38.102630528732185 40.0 37.0 41.0 33.0 41.0 22-23 38.00409536348845 40.0 37.0 41.0 33.0 41.0 24-25 37.90495287765244 40.0 37.0 41.0 32.5 41.0 26-27 37.71525515651169 40.0 37.0 41.0 32.0 41.0 28-29 37.59752415809096 39.5 37.0 41.0 32.0 41.0 30-31 37.38274619422661 39.0 36.0 41.0 31.5 41.0 32-33 37.175236464476605 39.0 36.0 41.0 31.0 41.0 34-35 37.01540592073622 39.0 36.0 41.0 31.0 41.0 36-37 37.02150112732073 39.0 36.0 41.0 31.0 41.0 38-39 36.90485822364269 39.0 36.0 41.0 30.0 41.0 40-41 36.746190986209506 39.0 35.0 41.0 30.0 41.0 42-43 36.537417156423 39.0 35.0 40.5 30.0 41.0 44-45 36.27800428415986 39.0 35.0 40.0 30.0 41.0 46-47 35.91598612423381 38.5 35.0 40.0 28.5 41.0 48-49 35.571512042377705 38.0 35.0 40.0 27.5 41.0 50-51 35.21069556200605 38.0 34.0 40.0 26.5 41.0 52-53 34.74024449727635 38.0 33.5 40.0 26.0 41.0 54-55 34.4409601156996 38.0 33.0 40.0 24.5 41.0 56-57 34.105063904247665 37.0 33.0 40.0 24.0 41.0 58-59 33.6681333205991 37.0 32.5 40.0 23.0 41.0 60-61 33.29147175899554 36.5 32.0 39.0 22.5 40.5 62-63 32.850837048432155 36.0 31.0 39.0 21.5 40.0 64-65 32.35486248733683 35.5 31.0 39.0 20.0 40.0 66-67 31.853650233820986 35.0 30.5 38.0 18.5 40.0 68-69 31.31395779966095 35.0 30.0 38.0 17.0 40.0 70-71 30.72803839371293 34.0 29.0 37.0 13.0 39.0 72-73 30.274913105913573 34.0 29.0 36.5 9.5 39.0 74-75 29.754116340863586 34.0 28.5 36.0 7.5 39.0 76-77 28.47193772959993 31.5 27.0 34.5 7.0 36.5 78-79 29.174428068069027 33.5 28.5 35.0 6.5 37.0 80-81 28.882536420475283 33.5 28.0 35.0 2.0 37.0 82-83 28.397416030807747 33.0 27.0 35.0 2.0 36.0 84-85 27.865288583873685 33.0 26.0 35.0 2.0 36.0 86-87 27.285457221504025 32.5 26.0 35.0 2.0 35.5 88-89 26.63712654303091 32.0 25.0 34.0 2.0 35.0 90-91 26.07463084936198 32.0 24.0 34.0 2.0 35.0 92-93 25.319083783295188 31.0 21.0 34.0 2.0 35.0 94-95 24.536221616588158 31.0 18.5 34.0 2.0 35.0 96-97 23.511156893711224 31.0 4.5 34.0 2.0 35.0 98-99 22.243759657267212 30.0 2.0 34.0 2.0 35.0 100-101 18.54515320305758 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26724930732858 1101 2 0.2774275362067229 1101 3 0.3295413161159573 1101 4 0.2512009385459777 1101 5 0.1281605447450218 1101 6 0.12884630118055185 1101 7 0.201444300587859 1101 8 0.2888559584383259 1101 9 0.24636849703477992 1101 10-11 0.33417622152103377 1101 12-13 0.2981218469562563 1101 14-15 0.4400083096577987 1101 16-17 0.5060824204239154 1101 18-19 0.4213997913540979 1101 20-21 0.4916647697963299 1101 22-23 0.4630061052930685 1101 24-25 0.44271623363638213 1101 26-27 0.4594683360806542 1101 28-29 0.5244569976213072 1101 30-31 0.39547991633334334 1101 32-33 0.4603060095664091 1101 34-35 0.438435780978935 1101 36-37 0.51785838920825 1101 38-39 0.4976809053419018 1101 40-41 0.4295720762247086 1101 42-43 0.4691304284457516 1101 44-45 0.42596616920019414 1101 46-47 0.5551204719316942 1101 48-49 0.5496871583710643 1101 50-51 0.6095489364635469 1101 52-53 0.6476059989221028 1101 54-55 0.5669702688820095 1101 56-57 0.6646895045530385 1101 58-59 0.7728109736628284 1101 60-61 0.746412354528097 1101 62-63 0.6327114546209387 1101 64-65 0.6661528600118203 1101 66-67 0.48054702227307544 1101 68-69 0.7188157454834645 1101 70-71 0.5979685893159967 1101 72-73 0.44365939808835364 1101 74-75 0.6868460496314057 1101 76-77 0.5202433632590697 1101 78-79 0.43963750752448405 1101 80-81 0.42824802622091696 1101 82-83 0.49684352861449455 1101 84-85 0.735778600184279 1101 86-87 0.5773935039301854 1101 88-89 0.5252143368074833 1101 90-91 0.47732016602864036 1101 92-93 0.4991610579782275 1101 94-95 0.6812389063372954 1101 96-97 0.8745536174181332 1101 98-99 0.6468136949901115 1101 100-101 0.4420960950232953 1102 1 0.31877668438898965 1102 2 0.28247282414559294 1102 3 0.32729679875470197 1102 4 0.23302199830025216 1102 5 0.15435908350821137 1102 6 0.23348154460416026 1102 7 0.3111083281462115 1102 8 0.27777352893832585 1102 9 0.358958449018715 1102 10-11 0.4028009145127669 1102 12-13 0.38712655004031404 1102 14-15 0.4365015013384763 1102 16-17 0.4497940709593635 1102 18-19 0.502056007611948 1102 20-21 0.4431340834731259 1102 22-23 0.4759505264821513 1102 24-25 0.49569575791672804 1102 26-27 0.38631709972067796 1102 28-29 0.3193186507323489 1102 30-31 0.3137425133619587 1102 32-33 0.35398612783367156 1102 34-35 0.47417539395973307 1102 36-37 0.4317771099733889 1102 38-39 0.4752009112032525 1102 40-41 0.537130868526333 1102 42-43 0.5041034272219704 1102 44-45 0.5068564394074784 1102 46-47 0.5308264346560989 1102 48-49 0.6020914250545317 1102 50-51 0.4831357982351818 1102 52-53 0.34660336781563217 1102 54-55 0.5736263656123057 1102 56-57 0.5183977217971147 1102 58-59 0.6066553622944113 1102 60-61 0.5966222028981392 1102 62-63 0.5877219595423284 1102 64-65 0.465964341558319 1102 66-67 0.5073379475813518 1102 68-69 0.6177545133694586 1102 70-71 0.5260647029701957 1102 72-73 0.41625733191209946 1102 74-75 0.506268238946042 1102 76-77 0.5240368001462734 1102 78-79 0.48019441839243626 1102 80-81 0.5708862793382572 1102 82-83 0.4149961002822664 1102 84-85 0.39288149990266064 1102 86-87 0.21786463252532684 1102 88-89 0.5148892068650319 1102 90-91 0.5140393172930047 1102 92-93 0.320925287133047 1102 94-95 0.3939531869789832 1102 96-97 0.5061436989319255 1102 98-99 0.5123796454416905 1102 100-101 0.3469852344699369 1103 1 0.2522687250538418 1103 2 0.30369779830296295 1103 3 0.26606208595770653 1103 4 0.1307160930555753 1103 5 0.15971774824323148 1103 6 0.18551391858336785 1103 7 0.22631653138167707 1103 8 0.21074799324075855 1103 9 0.2818982055430723 1103 10-11 0.29181830604372294 1103 12-13 0.24465104865480924 1103 14-15 0.4518946325572415 1103 16-17 0.44484053942363744 1103 18-19 0.45251924448866987 1103 20-21 0.3759063431174212 1103 22-23 0.4897000812940888 1103 24-25 0.5064723251476053 1103 26-27 0.5161954680690357 1103 28-29 0.45030612852121266 1103 30-31 0.4564600329719468 1103 32-33 0.5124979305050843 1103 34-35 0.45042672228507996 1103 36-37 0.5467288897121918 1103 38-39 0.4204450721214883 1103 40-41 0.534785004938712 1103 42-43 0.551535983040587 1103 44-45 0.6420850271889051 1103 46-47 0.6296508244950942 1103 48-49 0.7314950467860797 1103 50-51 0.6851708264521434 1103 52-53 0.7225428473340827 1103 54-55 0.7017704308814601 1103 56-57 0.5896054916679461 1103 58-59 0.566354502031821 1103 60-61 0.8178888727177238 1103 62-63 0.6947101814829253 1103 64-65 0.5555171169740944 1103 66-67 0.6643069142335101 1103 68-69 0.5441842306014486 1103 70-71 0.29670538287973613 1103 72-73 0.43477980944518535 1103 74-75 0.6431208582926331 1103 76-77 0.44440568610960085 1103 78-79 0.490270508150072 1103 80-81 0.36422081270878337 1103 82-83 0.4214711132880282 1103 84-85 0.3756983266118219 1103 86-87 0.6713286058323824 1103 88-89 0.6246894730609505 1103 90-91 0.6883323731314164 1103 92-93 0.7589926304533918 1103 94-95 0.7970718198245486 1103 96-97 0.7016227598915599 1103 98-99 0.4885980370894316 1103 100-101 0.2375472717277134 1104 1 0.2564240658241168 1104 2 0.30805533004554064 1104 3 0.2305633958646851 1104 4 0.19460633170652386 1104 5 0.08707097704328959 1104 6 0.14329068006971113 1104 7 0.1342111634105052 1104 8 0.17511693014733254 1104 9 0.30654254389126834 1104 10-11 0.27522599473616083 1104 12-13 0.29856938318222603 1104 14-15 0.4630724195711906 1104 16-17 0.4151472839803034 1104 18-19 0.34631744056096636 1104 20-21 0.3114364747266407 1104 22-23 0.40194955074779415 1104 24-25 0.34005732337404737 1104 26-27 0.4418884114400967 1104 28-29 0.37949805344018017 1104 30-31 0.3604914819853846 1104 32-33 0.2979692088440373 1104 34-35 0.2924763295941304 1104 36-37 0.35131506359829956 1104 38-39 0.361913479957785 1104 40-41 0.42715860980928966 1104 42-43 0.4964089541607848 1104 44-45 0.4187783355814503 1104 46-47 0.31964157478525834 1104 48-49 0.41162025993003937 1104 50-51 0.5985300033685448 1104 52-53 0.46872535057546116 1104 54-55 0.5978646542174388 1104 56-57 0.5797857579227639 1104 58-59 0.7036816498364615 1104 60-61 0.5513479877350989 1104 62-63 0.5010825708647886 1104 64-65 0.7163489067367195 1104 66-67 0.5818412292981243 1104 68-69 0.5008547989525383 1104 70-71 0.16475667894027168 1104 72-73 0.19089345061173546 1104 74-75 0.0909067382872557 1104 76-77 0.23995336124142597 1104 78-79 0.23550496299119672 1104 80-81 0.3438747311135977 1104 82-83 0.182599348285013 1104 84-85 0.33403140613261684 1104 86-87 0.4973465542055102 1104 88-89 0.4939132659011882 1104 90-91 0.4210774939563322 1104 92-93 0.41117214604786767 1104 94-95 0.6024699204945669 1104 96-97 0.41655762066093516 1104 98-99 0.40365135412253395 1104 100-101 0.07946228029564395 1105 1 0.13877125702045134 1105 2 0.2300940112699621 1105 3 0.1750352344879076 1105 4 0.1573985970666527 1105 5 0.11501066160055728 1105 6 0.13751297283318564 1105 7 0.22100138327257213 1105 8 0.17418368280100793 1105 9 0.18308048491557116 1105 10-11 0.2548237342557158 1105 12-13 0.29471417885422824 1105 14-15 0.45094739318324173 1105 16-17 0.3662549537286566 1105 18-19 0.30966371355693667 1105 20-21 0.23798919523238027 1105 22-23 0.22841620250865446 1105 24-25 0.3019785913153399 1105 26-27 0.2832768779607804 1105 28-29 0.34767808624928875 1105 30-31 0.4311750764711917 1105 32-33 0.38211121536261317 1105 34-35 0.397231310089893 1105 36-37 0.5388821759306026 1105 38-39 0.5148802212203307 1105 40-41 0.44010219209254586 1105 42-43 0.4603153650450835 1105 44-45 0.4637181576792955 1105 46-47 0.24342241803604026 1105 48-49 0.17538345697040114 1105 50-51 0.15860831678672582 1105 52-53 0.11126770894824034 1105 54-55 0.11875198485807203 1105 56-57 0.13068897817782954 1105 58-59 0.08536103361903002 1105 60-61 0.0020724609736504362 1105 62-63 0.19375412152218274 1105 64-65 0.15095048156513968 1105 66-67 0.24720467859279793 1105 68-69 0.014914087187872127 1105 70-71 -0.07317458153549694 1105 72-73 0.04632844329666952 1105 74-75 -0.054499061020763406 1105 76-77 0.032151622969319504 1105 78-79 0.1513736565496835 1105 80-81 0.20804316013497015 1105 82-83 0.2811582596770066 1105 84-85 0.38056436939189453 1105 86-87 0.35240436917397844 1105 88-89 0.31567539471718575 1105 90-91 0.21178476682100467 1105 92-93 0.33041922175024396 1105 94-95 0.3184634037017986 1105 96-97 0.27296498284895776 1105 98-99 0.2575978100452403 1105 100-101 -0.17091021613710922 1106 1 0.12762990311201605 1106 2 0.16658016725089908 1106 3 0.16940010597182464 1106 4 0.0964275298083308 1106 5 0.024563562935306038 1106 6 -0.00536494763855444 1106 7 0.04670980462721985 1106 8 0.10622064105778861 1106 9 -0.019038620688618835 1106 10-11 0.07461556839169248 1106 12-13 0.09534862701763558 1106 14-15 0.22011508920034117 1106 16-17 0.046566661109530116 1106 18-19 0.29039578405867417 1106 20-21 0.14690044791271362 1106 22-23 0.20306551213263901 1106 24-25 0.12511303010598596 1106 26-27 0.046109795915107554 1106 28-29 0.18887428959468622 1106 30-31 0.2760450739769311 1106 32-33 0.2991019041243135 1106 34-35 0.06522675955601187 1106 36-37 -0.24416691995016748 1106 38-39 -0.06199273896216795 1106 40-41 0.2807418735684095 1106 42-43 0.3900794822527516 1106 44-45 0.28463406233993993 1106 46-47 -0.08051829931810772 1106 48-49 0.03935851835100124 1106 50-51 0.15838987616387357 1106 52-53 -0.002317004221907837 1106 54-55 -0.18128784389153907 1106 56-57 0.06805556785548106 1106 58-59 0.04156098717420775 1106 60-61 -0.28812039309588755 1106 62-63 -0.2684277529563843 1106 64-65 -0.5612707034041158 1106 66-67 -0.32135397497492946 1106 68-69 -1.0550069808754046 1106 70-71 -0.7981726683881938 1106 72-73 -0.48320547873469266 1106 74-75 -0.3483692929559652 1106 76-77 -0.3730702326490345 1106 78-79 -0.4486278761735143 1106 80-81 -0.9537722170704122 1106 82-83 -0.564351677331377 1106 84-85 -0.3828134923070685 1106 86-87 -0.23931560648770045 1106 88-89 -0.2121518014380861 1106 90-91 -0.24815811884026928 1106 92-93 -0.4225511186223656 1106 94-95 -0.29804346278786653 1106 96-97 -0.29397113435077316 1106 98-99 -0.39969752233846734 1106 100-101 -0.7649733837400596 1107 1 0.1350119531398981 1107 2 0.18829774291044288 1107 3 0.2441398053149939 1107 4 0.1530507056924506 1107 5 0.08235092925453102 1107 6 0.12267096617070194 1107 7 0.10829977936636226 1107 8 0.20670647285377441 1107 9 0.28926194371158687 1107 10-11 0.2461939376007507 1107 12-13 0.18078760234175917 1107 14-15 0.24251789181603556 1107 16-17 0.24709734023416274 1107 18-19 0.28365017667906756 1107 20-21 0.2266817273197006 1107 22-23 0.316653568022339 1107 24-25 0.20770111894648835 1107 26-27 0.29829267972672113 1107 28-29 0.40187545742254116 1107 30-31 0.23487882090704204 1107 32-33 0.39228581625778247 1107 34-35 0.42334562380597873 1107 36-37 0.42775154863338827 1107 38-39 0.30429781780800624 1107 40-41 0.37867733449666474 1107 42-43 0.33985796298102855 1107 44-45 0.29189179796922105 1107 46-47 0.19379490107370856 1107 48-49 0.10468295325539856 1107 50-51 0.1478901237228385 1107 52-53 0.30552738410631264 1107 54-55 0.237386468217629 1107 56-57 0.19714612137336474 1107 58-59 0.0033465056807884253 1107 60-61 -0.02675632393685845 1107 62-63 0.12229668117773684 1107 64-65 0.05892880301851022 1107 66-67 0.19592444263329512 1107 68-69 0.24052461188517427 1107 70-71 0.0527924478361399 1107 72-73 0.10929160169565577 1107 74-75 0.10068822845029146 1107 76-77 -0.008753621634991049 1107 78-79 0.056302976349382305 1107 80-81 0.0744081025695067 1107 82-83 0.00231207939411604 1107 84-85 0.004861601634818413 1107 86-87 0.0813207548533903 1107 88-89 -0.11508547146872772 1107 90-91 -0.0628933976404511 1107 92-93 -0.1340992415605342 1107 94-95 -0.37260546314989895 1107 96-97 -0.0743505992382687 1107 98-99 -0.10936359803032047 1107 100-101 -0.4386997602599152 1108 1 0.1608408336436007 1108 2 0.19750972064156258 1108 3 0.14896939226438377 1108 4 0.06960638591489499 1108 5 0.017963979696894228 1108 6 0.029255352692359793 1108 7 0.021767176918366715 1108 8 0.14386819049191502 1108 9 0.16484295106117486 1108 10-11 0.15918354828210113 1108 12-13 0.15386675511712156 1108 14-15 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-0.45859124701933496 2316 84-85 -0.48932194874406676 2316 86-87 -0.35729092703406096 2316 88-89 -0.3221533643789023 2316 90-91 -0.5585599314984151 2316 92-93 -0.5972002626915156 2316 94-95 -0.4818903369724552 2316 96-97 -0.3050703320853039 2316 98-99 -0.25165369288621875 2316 100-101 0.012713847960618807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 12.0 4 44.0 5 109.0 6 214.0 7 565.0 8 1264.0 9 2141.0 10 3488.0 11 5473.0 12 7673.0 13 11112.0 14 14355.0 15 18871.0 16 23425.0 17 29162.0 18 28223.0 19 24168.0 20 24071.0 21 25769.0 22 28238.0 23 31805.0 24 37050.0 25 44052.0 26 52789.0 27 63127.0 28 76320.0 29 90185.0 30 107746.0 31 128226.0 32 155217.0 33 190034.0 34 240443.0 35 313975.0 36 416980.0 37 482499.0 38 242838.0 39 10059.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.173353494497576 19.49849047091645 15.1417483795288 23.18640765505717 2 15.769300593633421 20.90012107446421 39.136535686937194 24.194042644965172 3 19.689838660163044 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22-23 22.589733706719244 26.262530997056345 28.64707868732796 22.500656608896456 24-25 22.574546141699194 26.41580066612671 28.78120027990352 22.228452912270576 26-27 22.272221479330632 26.606914304271413 28.880264903011714 22.240599313386237 28-29 22.073570895000564 27.107850995828404 28.716679230352042 22.10189887881899 30-31 22.32601569721632 26.53929591060568 28.63906294235827 22.49562544981973 32-33 22.17641119748408 26.592233936958724 28.588103270082854 22.643251595474347 34-35 22.75367781880826 26.549408564094257 28.44978124973349 22.247132367363996 36-37 22.666679400906325 26.28901346083036 28.618117264269184 22.42618987399413 38-39 22.243192832894895 26.672558752578784 28.619488364096803 22.464760050429526 40-41 22.625414666177736 26.545935205478283 28.46432378499612 22.36432634334786 42-43 22.30048113369568 26.52714454630155 28.30855522941043 22.863819090592337 44-45 22.435630572329405 26.548919332428177 28.28263999117245 22.73281010406997 46-47 22.705996118332862 26.912096270802564 27.868818069876834 22.513089540987743 48-49 22.587243709345675 26.80785747664348 27.87149566979224 22.7334031442186 50-51 22.471237119380024 27.033765046576598 27.916349732069463 22.57864810197392 52-53 22.839449744689997 27.081842461618226 27.439105920395125 22.639601873296655 54-55 22.46039028150614 26.850801403949205 27.702192903166388 22.986615411378263 56-57 22.593315209790806 26.90537668884925 27.624013807547083 22.87729429381287 58-59 22.881694426157935 26.697171976955588 27.521889805677873 22.899243791208605 60-61 22.43184058559281 26.91584832163944 27.93011293673019 22.722198156037564 62-63 22.344912389612958 27.005522336640826 27.84221261848806 22.80735265525816 64-65 22.145030408666557 27.091700122453293 27.646236181367318 23.117033287512832 66-67 22.25831016013596 26.916041821758675 27.528070054259818 23.297577963845548 68-69 22.390694560109996 26.770757421152098 27.64840385516344 23.190144163574466 70-71 22.394028368252446 26.869409440439224 27.410421430637495 23.326140760670842 72-73 22.450144189632777 26.704356592243588 27.576862053610334 23.2686371645133 74-75 22.170186204050168 27.202692608118756 27.342285954027147 23.284835233803932 76-77 22.292912375969138 26.769023750481796 27.509593311798834 23.428470561750228 78-79 22.394439460659747 26.926149406664322 27.45793439368564 23.221476738990287 80-81 22.34907169584779 27.04718097752491 27.386029900842175 23.21771742578513 82-83 22.284843998894843 26.935883835713625 27.440845104051903 23.33842706133963 84-85 22.27266153086258 26.891934577169412 27.442014124825693 23.393389767142313 86-87 22.254278376660878 27.018642332793597 27.239080507603024 23.4879987829425 88-89 22.116795242670793 27.08942402247957 27.03009043468888 23.763690300160757 90-91 22.102056221792658 26.906808785119864 27.250166203956216 23.740968789131266 92-93 22.281126122661366 26.753983304257538 27.331906291406145 23.632984281674947 94-95 22.011239440746493 26.62199906232823 27.370686824961172 23.99607467196411 96-97 21.90080600873887 26.623682944882372 27.448673650029164 24.026837396349595 98-99 21.95500026690739 26.74338704965347 27.06302783494447 24.238584848494668 100-101 21.882697568107833 26.487573290782795 27.382284074980312 24.247445066129057 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 426.5 2 320.0 3 435.0 4 374.5 5 358.0 6 675.5 7 1530.0 8 2316.5 9 2527.0 10 2274.5 11 1900.0 12 1912.5 13 2154.5 14 2393.5 15 2555.5 16 2735.0 17 2931.5 18 3184.5 19 3572.5 20 4010.0 21 4578.0 22 5530.5 23 6646.0 24 7950.0 25 9847.5 26 12358.5 27 16078.5 28 20175.5 29 25135.5 30 30966.5 31 36037.5 32 42961.5 33 48505.0 34 52372.0 35 58065.0 36 64397.0 37 70856.5 38 75997.5 39 79642.0 40 84239.0 41 90260.5 42 95360.0 43 100477.0 44 105600.5 45 110929.5 46 114412.5 47 121230.5 48 131761.5 49 130340.0 50 120725.5 51 112329.0 52 105364.0 53 101453.5 54 99145.5 55 92473.5 56 82584.5 57 74730.5 58 68212.5 59 60373.5 60 50095.5 61 40404.5 62 32419.5 63 24264.0 64 17745.0 65 13752.0 66 10440.5 67 7969.5 68 5981.0 69 4355.0 70 3370.5 71 2715.0 72 2204.5 73 1915.5 74 1581.0 75 1256.0 76 891.0 77 549.5 78 329.5 79 195.5 80 119.5 81 70.0 82 38.5 83 22.5 84 12.0 85 9.0 86 8.0 87 5.5 88 2.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.163006143130506E-4 2 0.044410638087409145 3 0.0010232865918757867 4 0.0 5 0.08967401500138143 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8760254184389423E-4 26-27 0.008254511841131346 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010403413684070497 36-37 5.1164329593789334E-5 38-39 0.003888489049127989 40-41 0.005286980724691565 42-43 2.899312010314729E-4 44-45 1.7054776531263112E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.527388265631555E-5 68-69 8.697936030944186E-4 70-71 6.821910612505245E-5 72-73 1.7054776531263112E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013643821225010487 82-83 6.821910612505244E-4 84-85 0.005252871171629038 86-87 0.0023535591613143094 88-89 3.4109553062526225E-5 90-91 0.0031039693286898863 92-93 8.356840500318925E-4 94-95 1.7054776531263112E-5 96-97 0.0 98-99 5.1164329593789334E-5 100-101 1.7054776531263112E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2931730.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.08553301447517 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97457402316059 37.27945010477432 2 14.549217484958538 14.865090603252574 3 5.153568181161713 7.898183323832562 4 2.441307499281757 4.98861979412176 5 1.336439747963901 3.413636843323337 6 0.8170858176625847 2.5044758708316843 7 0.5334527086560542 1.907620117179704 8 0.3563193377629371 1.4562210634387562 9 0.27655700667045846 1.2715255885183323 >10 1.4081848766719796 12.834700512219321 >50 0.09793361059499686 3.4583570059334114 >100 0.04993731076259493 4.587079017133769 >500 0.0032802140729828013 1.1429743160899766 >1k 0.0018744080415592917 1.4462103060930227 >5k 2.677725773656131E-4 0.9458555332573677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8878 0.3028246120891078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7234 0.2467485068543147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6257 0.21342347351222657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5289 0.1804054261477012 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3298 0.11249330600021148 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4109553062526225E-5 0.0 12-13 0.0 0.0 0.0 3.4109553062526225E-5 0.0 14-15 0.0 0.0 0.0 8.527388265631556E-5 0.0 16-17 0.0 0.0 0.0 1.7054776531263112E-4 0.0 18-19 0.0 0.0 0.0 1.7054776531263112E-4 0.0 20-21 0.0 0.0 0.0 2.728764245002098E-4 0.0 22-23 0.0 0.0 0.0 4.945885194066302E-4 0.0 24-25 0.0 0.0 0.0 9.891770388132603E-4 0.0 26-27 0.0 0.0 0.0 0.0016202037704699955 0.0 28-29 0.0 0.0 0.0 0.004093146367503147 0.0 30-31 0.0 0.0 0.0 0.013029849269885017 0.0 32-33 0.0 0.0 0.0 0.03322270468290054 0.0 34-35 0.0 0.0 0.0 0.062028222244203936 0.0 36-37 0.0 0.0 0.0 0.11150412896139822 0.0 38-39 0.0 0.0 0.0 0.18703973421836256 0.0 40-41 0.0 0.0 0.0 0.26862978514392527 0.0 42-43 0.0 0.0 0.0 0.35427887288392856 0.0 44-45 3.4109553062526225E-5 0.0 0.0 0.4584835574899462 0.0 46-47 3.4109553062526225E-5 0.0 0.0 0.5847571229274182 0.0 48-49 3.4109553062526225E-5 0.0 0.0 0.7157719162405815 0.0 50-51 3.4109553062526225E-5 0.0 0.0 0.8611468313930682 0.0 52-53 3.4109553062526225E-5 0.0 0.0 1.0018999021055828 0.0 54-55 3.4109553062526225E-5 0.0 0.0 1.1619248702984244 0.0 56-57 3.4109553062526225E-5 1.7054776531263112E-5 0.0 1.3531941890965402 0.0 58-59 3.4109553062526225E-5 3.4109553062526225E-5 0.0 1.5492729548764723 0.0 60-61 3.4109553062526225E-5 3.4109553062526225E-5 0.0 1.7613832105957916 0.0 62-63 3.4109553062526225E-5 6.821910612505245E-5 0.0 1.9741415478232989 3.4109553062526225E-5 64-65 3.4109553062526225E-5 6.821910612505245E-5 0.0 2.1998615152145664 3.4109553062526225E-5 66-67 3.4109553062526225E-5 6.821910612505245E-5 0.0 2.457917338909108 3.4109553062526225E-5 68-69 3.4109553062526225E-5 6.821910612505245E-5 0.0 2.752197507956053 3.4109553062526225E-5 70-71 3.4109553062526225E-5 6.821910612505245E-5 0.0 3.064743342667981 3.4109553062526225E-5 72-73 3.4109553062526225E-5 6.821910612505245E-5 0.0 3.379438079222848 3.4109553062526225E-5 74-75 3.4109553062526225E-5 6.821910612505245E-5 0.0 3.700733014295314 6.821910612505245E-5 76-77 3.4109553062526225E-5 6.821910612505245E-5 0.0 4.049110934499425 6.821910612505245E-5 78-79 3.4109553062526225E-5 6.821910612505245E-5 0.0 4.445242228991074 6.821910612505245E-5 80-81 3.4109553062526225E-5 6.821910612505245E-5 0.0 4.859570970041579 6.821910612505245E-5 82-83 3.4109553062526225E-5 6.821910612505245E-5 0.0 5.2855651782394695 6.821910612505245E-5 84-85 6.821910612505245E-5 6.821910612505245E-5 0.0 5.7186541734743646 6.821910612505245E-5 86-87 6.821910612505245E-5 6.821910612505245E-5 0.0 6.159315489489141 6.821910612505245E-5 88-89 6.821910612505245E-5 6.821910612505245E-5 0.0 6.643312992669857 6.821910612505245E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12350 0.0 24.953808 1 TACATGG 12230 0.0 24.49869 2 GGTATCA 12665 0.0 24.220509 1 GTATCAA 16620 0.0 23.836515 1 ACATGGG 12770 0.0 23.005993 3 TAGGACG 345 0.0 22.04636 4 GAGTACT 9230 0.0 21.22623 12-13 GTACTTT 9560 0.0 20.245123 14-15 ATCAACG 19500 0.0 20.161102 3 TCAACGC 19525 0.0 20.110598 4 TATCAAC 19615 0.0 20.027214 2 CAACGCA 19640 0.0 19.968637 5 AACGCAG 20060 0.0 19.604242 6 AGTACTT 9480 0.0 19.514156 12-13 GGACCGA 895 0.0 19.104256 6 TAAGGTG 1375 0.0 19.014984 5 CATGGGG 6110 0.0 18.90606 4 AGAGTAC 16170 0.0 18.66621 10-11 TAGCCCT 2035 0.0 18.220745 4 CATGGGA 6920 0.0 17.92959 4 >>END_MODULE